| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| Also , ectopic induction of a beta catenin signaling inhibitor , ICAT , inhibited expression of beta 3 tubulin . ^^^ |
|
| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| Inhibition of Wnt signaling by ICAT , a novel beta catenin interacting protein . ^^^ Here , we identify a novel beta catenin interacting protein , ICAT , that was found to inhibit the interaction of beta catenin with TCF 4 and represses beta catenin TCF 4 mediated transactivation . ^^^ These results suggest that ICAT negatively regulates Wnt signaling via inhibition of the interaction between beta catenin and TCF and is integral in development and cell proliferation . . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| ICAT inhibits the interaction between beta catenin and TCF transcription factors . ^^^ As ICAT might be a tumor suppressor gene with the potential to negatively regulate the WNT beta catenin TCF signaling pathway , ICAT related gene in the human genome draft sequence was searched for . ^^^ As LZIC contains ICAT homologous domain , LZIC might inhibit the interaction between beta catenin and TCF transcription factors , just like ICAT , and , up regulation of LZIC in gastric cancer might be due to a negative feed back mechanism to inhibit the WNT beta catenin TCF signaling pathway . . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| LEF 1 beta cat transactivation in vitro was also repressed by inhibitor of beta catenin and Tcf 4 ( ICAT ) , a physiological inhibitor of Wnt / Wg signaling that interacts with ARM repeats 11 and 12 , and by the nonsteroidal anti inflammatory compound , sulindac . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| Overexpression of Icat induces G ( 2 ) arrest and cell death in tumor cell mutants for adenomatous polyposis coli , beta catenin , or Axin . ^^^ We have recently identified ICAT , a beta catenin interacting protein that interferes with the interaction between beta catenin and TCF 4 , thereby negatively regulating Wnt signaling . ^^^ We found that Icat inhibits proliferation of colorectal tumor cells mutated in APC or beta catenin and hepatocellular carcinoma cells mutated in AXIN : By contrast , Icat did not inhibit growth of either normal or tumor cells containing the wild type APC , beta catenin , and Axin genes . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| We investigated 37 malignant melanomas ( 15 primary tumors and 22 metastases ) for alterations of 4 genes encoding members of this pathway , i . e . , CTNNB 1 ( beta catenin gene , 3p22 . 1 ) , APC ( adenomatous polyposis coli gene , 5q22 . 2 ) , BTRC ( beta transducin repeat containing protein gene , 10q24 . 3 ) and ICAT ( inhibitor of beta catenin and Tcf 4 , 1p36 . 2 ) . ^^^ Molecular genetic analysis of malignant melanomas for aberrations of the WNT signaling pathway genes CTNNB 1 , APC , ICAT and BTRC . ^^^ Taken together , our results indicate that the Wnt pathway may be altered in malignant melanomas by different mechanisms , including rare somatic mutations in CTNNB 1 , APC or ICAT , as well as low or absent expression of ICAT transcripts . . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| The crystal structure of the beta catenin / ICAT complex reveals the inhibitory mechanism of ICAT . ^^^ We have determined the crystal structure at 2 . 5 A resolution of a complex between beta catenin and ICAT , a protein that prevents the interaction between beta catenin and Tcf / Lef family transcription factors . ^^^ ICAT contains a 3 helix bundle that binds armadillo repeats 10 12 and a C terminal tail that , similar to Tcf and E cadherin , binds in the groove formed by armadillo repeats 5 9 of beta catenin . ^^^ We show that ICAT selectively inhibits beta catenin / Tcf binding in vivo , without disrupting beta catenin / cadherin interactions . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| ICAT inhibits beta catenin binding to Tcf / Lef family transcription factors and the general coactivator p 300 using independent structural modules . ^^^ The crystal structure of ICAT , an inhibitor of beta catenin mediated transcription , bound to the armadillo repeat domain of beta catenin , has been determined . ^^^ ICAT contains an N terminal helilical domain that binds to repeats 11 and 12 of beta catenin , and an extended C terminal region that binds to repeats 5 10 in a manner similar to that of Tcfs and other beta catenin ligands . ^^^ Full length ICAT dissociates complexes of beta catenin , Lef 1 , and the transcriptional coactivator p 300 , whereas the helical domain alone selectively blocks binding to p 300 . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| Mutation and expression of the beta catenin interacting protein ICAT in human colorectal tumors . ^^^ We have recently identified the ICAT gene , which encodes a small protein that interacts with beta catenin and represses Wnt signaling . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| ICAT , an inhibitor of T cell factor 4 ( TCF 4 ) , and E cadherin binding to beta catenin also blocked binding of the androgen receptor LBD . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| Infrequent somatic mutations of the ICAT gene in various human cancers with frequent 1p LOH and / or abnormal nuclear accumulation of beta catenin . ^^^ ICAT , inhibitor of beta catenin and TCF4 / beta catenin interacting protein , was isolated and mapped to 1p36 , a frequent target for LOH in many human cancers . ^^^ We studied the precise localization and genomic structure of the ICAT gene and analyzed its mutations in 178 human tumors developed in organs with frequent nuclear accumulation of the CTNNB 1 protein and / or frequent LOHs of 1p36 , but no genetic alterations were observed . ^^^ Our results imply that 1 ) genetic alteration of the ICAT gene does not play an important role in abnormal accumulation of CTNNB 1 that would cause up regulation of the Wnt signalling pathway , 2 ) mechanisms other than genetic alteration may have inactivated ICAT function , or 3 ) gene ( s ) on 1p36 other than ICAT may be the responsible tumor suppressor gene for tumors that show frequent 1p36 LOH . . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| LZIC is closely related to ICAT , a physiological inhibitor of the Wnt pathway that interacts physically and functionally with beta catenin to prevent the transcription of Wnt target genes . ^^^ LZIC ' s ICAT homologous region is highly similar to ICAT with particular conservation of residues that are used by ICAT for beta catenin binding . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| Several components of the Wnt / beta catenin pathway , such as APC , ICAT , LEF 1 and beta catenin are modified in melanoma tumors and cell lines , leading to activation of this signaling . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| Among these 30 genes we found a high proportion of genes associated with apoptotic cell death , cell proliferation and cell cycle signalling including complement lysis inhibitor ( clusterin / CLU ) , beta catenin interacting protein ( ICAT ) , peroxisome proliferator activated receptor alpha ( PPARalpha ) , TNF alpha converting enzyme ( ADAM17 / TACE ) , homeo box A 3 ( HOX 1 ) , inositol polyphosphatase 5 phosphatase type 4 ( PPI5PIV ) and inhibitor of p 53 induced apoptosis alpha ( IPIA Alpha / NM23 H 6 ) . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| The thermodynamics of beta catenin binding to E cadherin , Lef 1 , APC , axin , and the transcriptional inhibitor ICAT have been determined by isothermal titration calorimetry . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| To directly investigate the importance of catenin mediated Wnt signalling in early thymocytes , we have compared the expression of beta and gamma catenin and analysed distinct stages of T cell precursor maturation following overexpression of inhibitor of beta catenin and Tcf ( ICAT ) , which inhibits Wnt signalling by preventing binding of armadillo repeat containing proteins to Tcf / Lef . ^^^ |
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| Interacting proteins: Q9NSA3 and P35222 |
Pubmed |
SVM Score :0.0 |
| NA |
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