| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.94212099 |
| In both yeast two hybrid and in vitro binding assays , we found that Smurf 2 could interact with receptor activated Smads ( R Smads ) , including Smad 1 , Smad 2 , and Smad 3 but not Smad 4 . 0.94212099^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| At higher expression levels , Smurf 2 also decreases the protein levels of Smad 2 , but not Smad 3 . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| We show that in the presence of TGF beta signalling , Smad 2 interacts through its proline rich PPXY motif with the tryptophan rich WW domains of Smurf 2 , a recently identified E 3 ubiquitin ligases . ^^^ TGF beta also induces the association of Smurf 2 with the transcriptional co repressor SnoN and we show that Smad 2 can function to mediate this interaction . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Smurf 2 induces ubiquitin dependent degradation of SnoN , since it appears to be located close to SnoN through binding to the linker region of Smad 2 . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Smurf 2 ( Smad ubiquitination regulatory factor 2 ) was identified as the Smad ubiquitin ligase that induces the ubiquitination and degradation of Smad 2 . ^^^ This study was undertaken to elucidate the relationships between Smurf 2 expression and the clinicopathological characteristics of patients with esophageal squamous cell carcinoma ( SCC ) and the correlation between Smurf 2 and Smad 2 expression . ^^^ We also found an inverse correlation between the expression of Smurf 2 and Smad 2 . ^^^ We demonstrated that high level expression of Smurf 2 appears to correlate with tumor development and poor prognosis in patients with esophageal SCC and that alteration of Smad 2 expression in the TGF beta signaling pathway may be induced by enhancement of Smad 2 degradation mediated by high level expression of Smurf2 . . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| We also found that Smurf 2 , a ubiquitin ligase for Smad 2 , was increased in the nephritic glomerular extracts . ^^^ These data suggest that the decrease in Smad 2 resulted from enhanced ubiquitin dependent degradation of Smad 2 mediated by Smurf 2 , and is involved in the regulation of Smad 2 mediated TGF beta signaling in nephritic glomeruli . . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Since Aza unmasked responsiveness of ACs to BMP 2 and TGF beta , we examined the effect of Aza treatment on signaling via these pathways by assessing the expression of the TGF beta Smads ( 2 and 3 ) , the BMP 2 Smads ( 1 and 5 ) , and the Smad 2 and 3 degrading ubiquitin E 3 ligase Smurf 2 . ^^^ Aza treated ACs displayed less Smad 2 and 3 and increased Smad 1 , 5 , and Smurf 2 protein and showed a loss of TGF beta signaling on the P3TP luciferase reporter . ^^^ Suggesting that Aza induction of Smurf 2 may be responsible for the loss of Smad 2 and 3 protein via this pathway , immunoprecipitation and metabolic labeling experiments confirmed that Aza accelerated the ubiquitination and degradation of these targets . ^^^ Overall , Aza treated ACs represent a novel model for the study of mechanisms that regulate maturational potential of articular cartilage , with the data suggesting that maturation of these cells may be due to up regulation of Smad 1 and 5 coupled with a Smurf 2 dependent degradation of Smad 2 and 3 and loss of TGF beta signaling . . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| However , in contrast with Smurf 2 , NEDD 4 2 failed to induce ubiquitination of SnoN ( Ski related novel protein N ) , although NEDD 4 2 bound to SnoN via Smad 2 more strongly than Smurf 2 . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Smad ubiquitination regulatory factor 2 ( Smurf 2 ) , a ubiquitin ligase for Smads , plays critical roles in the regulation of transforming growth factor beta ( TGF beta ) Smad signaling via ubiquitin dependent degradation of Smad 2 and Smad 7 . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Smad 3 / chondrocytes lacked compensatory increases in Smad 2 , Smad 4 , TGFRII , Sno , or Smurf 2 and had reduced expression of TGF beta 1 and TGFRI . ^^^ |
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| Interacting proteins: Q96DE7 and Q15796 |
Pubmed |
SVM Score :0.0 |
| NA |
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