| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| We identified the homeodomain protein TGIF as a Smad 2 binding protein and a repressor of transcription . ^^^ Thus , upon entering the nucleus , a Smad 2 Smad4 complex may interact with coactivators , forming a transcriptional activation complex , or with TGIF and HDACs , forming a transcriptional repressor complex . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Heterozygous mutations in individuals with HPE affect the transcriptional repression domain of TGIF , the DNA binding domain or the domain that interacts with SMAD 2 . ( The latter is an effector in the signalling pathway of the neural axis developmental factor NODAL , a member of the transforming growth factor beta ( TGF beta ) family . ) Several of these mutations cause a loss of TGIF function . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| The Smad co repressor TGIF competes with the co activator p 300 for Smad 2 association , such that TGIF abundance helps determine the outcome of a TGF beta response . ^^^ Here we show that TGIF levels modulate sensitivity to TGF beta mediated growth inhibition , that TGIF is a short lived protein and that epidermal growth factor ( EGF ) signaling via the Ras Mek pathway causes the phosphorylation of TGIF at two Erk MAP kinase sites , leading to TGIF stabilization and favoring the formation of Smad 2 TGIF co repressor complexes in response to TGF beta . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Jun interacts with the corepressor TG interacting factor ( TGIF ) to suppress Smad 2 transcriptional activity . ^^^ This inhibitory activity is mediated through the transcriptional factor c Jun , which enhances the association of Smad 2 with the nuclear transcriptional corepressor TG interacting factor ( TGIF ) , thereby interfering with the assembly of Smad 2 and the coactivator p 300 in response to TGF beta signaling . ^^^ Interestingly , c Jun directly binds to the nuclear transcriptional corepressor TGIF and is required for TGIF mediated repression of Smad 2 transcriptional activity . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| Instead , we found that conophylline increased the expression of c Jun , which had been earlier shown to interact with the corepressor TGIF to suppress the transcriptional activity dependent on Smad 2 . ^^^ Conophylline attenuated the interaction between the Smad 2 complex and p 300 but enhanced that between the Smad 2 complex and TGIF . ^^^ In cells overexpressing c Jun , suppression of promoter activity induced by TGF beta and the enhancement of the association of the Smad 2 complex with TGIF were also observed . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| The novel E 3 ubiquitin ligase Tiul 1 associates with TGIF to target Smad 2 for degradation . ^^^ Here , we identify Tiul 1 ( for TGIF interacting ubiquitin ligase 1 ) , a novel E 3 ubiquitin ligase that inhibits TGF beta signaling by targeting both the activated receptor and Smad 2 for degradation . ^^^ Tiul 1 can also interact with Smad 2 and the nuclear corepressor TGIF upon activation of TGF beta signaling . ^^^ Like Smad 7 , the steady state levels of TGIF are not affected by Tiul 1 , but the interaction of Tiul 1 with TGIF allows this ubiquitin ligase to target Smad 2 for degradation . ^^^ In addition , silencing of Tiul 1 or TGIF genes by siRNA resulted in suppression of the TGF beta dependent degradation of Smad 2 and an enhancement of TGF beta mediated gene expression . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| The Tgif gene encodes a homeodomain protein that functions as a transforming growth factor beta ( TGF beta ) repressor by binding to Smad 2 . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| In this study , we report an unexpected role of TGIF in the inhibition of Smad 2 phosphorylation , which occurs by a mechanism independent of its association with Smad 2 . ^^^ |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15583 and Q15796 |
Pubmed |
SVM Score :0.0 |
| NA |
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