| Interacting proteins: P49006 and Q13561 |
Pubmed |
SVM Score :0.87949645 |
| Dynamitin was found to copurify with MacMARCKS both during MacMARCKS purification with conventional chromatography and during the immunoabsorption of MacMARCKS using anti MacMARCKS antibody . 0.87949645^^^ |
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| Interacting proteins: P49006 and Q13561 |
Pubmed |
SVM Score :1.5600246 |
| In this study , we used the fluorescent resonance energy transfer method to demonstrate in vivo interaction between MacMARCKS and dynamitin with cyan fluorescent protein ( CFP ) conjugated dynamitin as the donor fluorophore and yellow fluorescent protein ( YFP ) conjugated MacMARCKS as the acceptor fluorophore . 1.5600246^^^ In vivo interaction between dynamitin and MacMARCKS detected by the fluorescent resonance energy transfer method . 0.53565552^^^ |
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| Interacting proteins: P49006 and Q13561 |
Pubmed |
SVM Score :0.0 |
| Dynamitin , a subunit of the microtubule dependent motor complex , was implicated in cell adhesion by binding to MacMARCKS ( Macrophage enriched myristoylated alanine rice C kinase substrate ) . ^^^ However , how dynamitin is involved in cell adhesion is unclear despite the fact that both MacMARCKS and microtubules regulate beta ( 2 ) integrin activation . ^^^ Using the single particle tracking method , we found that integrin molecular mobility in cells expressing the fusion protein CFP ( cyan fluorescent protein ) dynamitin or CFP MB ( the MacMARCKS binding domain peptide of dynamitin ) increased 6 fold over the control cells , suggesting that disturbing dynamitin function dramatically altered the cytoskeletal constraint on beta ( 2 ) integrin molecules . ^^^ Further mechanistic studies revealed that overexpression of dynamitin stimulated the phosphorylation of endogenous MacMARCKS protein , which lead to the enhanced tyrosine phosphorylation of paxillin . ^^^ Although dynamitin acts at the point of MacMARCKS phosphorylation , it is upstream of RhoA , because its effect was blocked by RhoA inhibitor . ^^^ |
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