| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| This system has also been used to demonstrate that beta arrestin 1 and beta adrenergic receptor kinase 1 act synergistically to promote receptor desensitization . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Both cell types also express beta ARK 1 and the associated inhibitory proteins beta arrestin 1 and beta arrestin 2 , as assessed by immunoblotting . ^^^ Like the beta 2AR , the beta 1AR appears to bind either beta arrestin 1 or beta arrestin 2 and to react with rhodopsin kinase , beta ARK 1 , beta ARK 2 , and GRK5 . . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Two color FISH confirmed that the two genes coding for the functionally related proteins beta ARK 1 and beta arrestin 1 both map to 11q13 . . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have investigated the expression of the individual isoforms of beta arrestin and of beta ARK in left ventricles from failing and control human hearts . mRNAs for all four proteins , beta arrestin 1 , beta arrestin 2 , beta ARK 1 , and beta ARK 2 , were identified in human heart . ^^^ Quantitation by reverse transcription polymerase chain reactions showed that in heart failure there were no changes of the mRNA levels for beta arrestin 1 and beta arrestin 2 , a slight ( < 50 % ) increase of the mRNA for beta ARK 2 , and a threefold increase for beta ARK 1 mRNA . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Nucleotide sequence of PCR fragments from ROS 17 / 2 . 8 cells revealed 100 % identity with rat brain beta ARK 1 and beta arrestin 1 sequences . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Synergistic regulation of m 2 muscarinic acetylcholine receptor desensitization and sequestration by G protein coupled receptor kinase 2 and beta arrestin 1 . ^^^ We used a heterologous expression system to examine the ability of G protein coupled receptor kinase 2 ( GRK 2 ) and beta arrestin 1 to regulate the phosphorylation state and to promote desensitization and sequestration of the m 2 mAChR . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Expression patterns of the regulatory proteins G protein coupled receptor kinase 2 and beta arrestin 1 during rat postnatal brain development : effect of hypothyroidism . ^^^ G protein coupled receptor kinase 2 ( GRK 2 ) and beta arrestin 1 are key regulatory proteins that modulate the desensitization and resensitization of a wide variety of G protein coupled receptors ( GPCRs ) involved in brain functions . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| In addition , we found that G protein coupled receptor kinase 2 and beta arrestin 1 can associate with the platelet derived growth factor beta receptor . ^^^ Thus , platelet derived growth factor beta receptor signaling may be initiated by G protein coupled receptor kinase 2 / beta arrestin 1 that has been recruited to the platelet derived growth factor beta receptor by its tethering to a G protein coupled receptor ( s ) . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Regulation of lysophosphatidic acid receptor stimulated response by G protein coupled receptor kinase 2 and beta arrestin 1 in FRTL 5 rat thyroid cells . ^^^ The LPAr are endogenously expressed in the rat thyroid cell line FRTL 5 and we used the FRTL 5 cells permanently transfected to obtain moderate overexpression of G protein coupled receptor kinase 2 ( GRK 2 ) or beta arrestin 1 to study whether GRK 2 and beta arrestin 1 desensitise LPAr mediated signalling and regulate LPA stimulated functional effects . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Unexpectedly , we find that internalization of the EGFR by EGF is dependent upon Gbetagamma and beta arrestin proteins ; expression of minigenes encoding the carboxyl terminii of the G protein coupled receptor kinase 2 , or beta arrestin 1 , attenuates LPA , ISO , and EGF mediated internalization of EGFR . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| GRK 2 and beta arrestin 1 as negative regulators of thyrotropin receptor stimulated response . ^^^ The TSH induced cAMP accumulation in COS 7 cells transfected with TSH receptor was reduced by 35 45 % when cotransfected with GRK 2 and / or beta arrestin 1 , indicating that the TSH receptor can be regulated by a GRK / arrestin mechanism . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| In contrast , resensitization of a sequestration impaired beta2AR mutant ( Y326A ) was reestablished following the overexpression of either GRK 2 or beta arrestin 1 . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Therefore , we examined the effects of the neurotransmitter substance P ( SP ) on desensitization of the neurokinin 1 receptor ( NK 1 R ) and on the subcellular distribution of NK 1 R , Galphaq / 11 , GRK 2 and 3 , and beta arrestin 1 and 2 in cultured myenteric neurons . ^^^ NK 1 R was coexpressed with immunoreactive Galphaq / 11 , GRK 2 and 3 , and beta arrestin 1 and 2 in a subpopulation of neurons . ^^^ Thus , SP induces desensitization and endocytosis of the NK 1 R in neurons that may be mediated by GRK 2 and 3 and beta arrestin 1 and 2 . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Expression of G protein receptor kinase 2 ( GRK 2 ) , beta arrestin 1 and the regulatory subunits of cAMPdPK ( RI alpha and RII alpha ) , showed no change after MI . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Beta arrestin 1 transfection also induced a significantly decrease in GIP stimulated cAMP production , and this effect was greater with cotransfection of both GRK 2 and beta arrestin 1 than with either alone . ^^^ In betaTC 3 cells , expression of GRK 2 or beta arrestin 1 attenuated GIP induced insulin release and cAMP production , whereas glucose stimulated insulin secretion was not affected . ^^^ GRK 2 and beta arrestin 1 messenger RNAs were identified by Northern blot analysis to be expressed endogenously in betaTC 3 and L 293 cells . ^^^ Overexpression of GRK 2 enhanced agonist induced GIPR phosphorylation , but receptor endocytosis was not affected by cotransfection with GRKs or beta arrestin 1 . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| In vitro binding assays show that the residues 498 502 of GRK 2 bind the amino terminal domain of clathrin heavy chain to almost the same extent as beta arrestin 1 . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Myocardial GRK 2 , GRK 5 , beta arrestin 1 , and beta arrestin 2 mRNA levels , but not that of GRK 3 , were induced in the failing hearts . ^^^ Consistently , Western blot analysis of tissue extracts from the nonischemic region of the left ventricle revealed 3 . 0 , 2 . 6 , and 1 . 5 fold elevations of GRK 2 , GRK 5 , and beta arrestin 1 , respectively , 7 days after induction of myocardial infarction compared with the sham operated rats ( P < 0 . 05 ) . ^^^ Immunohistochemical analysis of myocardial tissue sections and Western blot analysis of isolated cells revealed localization of GRK 2 and beta arrestin 1 predominantly in endothelial cells . ^^^ In conclusion , myocardial mRNA and protein levels of GRK 2 , GRK 5 , and beta arrestin 1 are induced in postinfarction failure in rats . ^^^ The immunohistochemical analysis suggests that GRK 2 and beta arrestin 1 may act as primary regulators of endothelial function . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| The tonic internalization of mGluR1a is insensitive to antagonist treatment , dominant negative mutants of GRK 2 , beta arrestin 1 , and dynamin as well as treatments that disrupt caveolae , but is blocked by hypertonic sucrose and concanavalin A treatment . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Fourth , the over expression of C terminal GRK 2 peptide ( which sequesters G protein betagamma subunits ) or beta arrestin 1 clathrin binding domain ( amino acids : 319 418 ) or pre treatment of cells with pertussis toxin reduced the LPA and NGF dependent stimulation of p42 / p44 MAPK . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| Using sucrose gradient fractionation , we show that FSH induces the recruitment of the endogenous GRK 2 and beta arrestin 1 / 2 from the cytoplasm to the plasma membrane of rat primary Sertoli cells . ^^^ |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P25098 and P49407 |
Pubmed |
SVM Score :0.0 |
| NA |
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