| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Subsequently , the kinase cascade ( Raf 1 , Mek 2 , and p44MAPK [ mitogen activated protein kinase ] ) is activated . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Indeed , MEK 1 and MEK 2 are the only known activators of ERK 1 and ERK 2 . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Following EGF stimulation of B82L cells expressing a kinase defective EGF receptor mutant ( K721M ) , we found that ERK 2 and ERK 1 MAP kinases , as well as MEK 1 and MEK 2 were all activated , and SHC became prominently tyrosine phosphorylated . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| In this paper , we have cloned a C . elegans mek gene , mek 2 , and demonstrated that the MEK 2 protein possesses the biochemical properties of MAP kinase kinases : The C . elegans MEK 2 protein can phosphorylate and activate a human MAP kinase ( ERK 1 ) , and MEK 2 itself can be phosphorylated and activated by immunoprecipitated mammalian Raf . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| We now demonstrate that three recombinant MEKs ( MEK 1 , MEK 2 , MEK 3 ) show remarkably different activity toward recombinant ERK 1 and ERK 2 . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Recombinant MEK 2 produced in bacteria phosphorylates a kinase inactive Erk 1 on tyrosine and threonine , whereas a kinase inactive mutant MEK 2 does not . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Both MEK 1 and MEK 2 were expressed in Escherichia coli and shown to be able to activate recombinant human ERK 1 in vitro . ^^^ The purified MEK 2 protein stimulated threonine and tyrosine phosphorylation on ERK 1 and concomitantly activated ERK 1 kinase activity more than 100 fold . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Characterization of ERK 1 activation site mutants and the effect on recognition by MEK 1 and MEK 2 . ^^^ To discern MEK 1 and MEK 2 specificity for their substrate , extracellular signal regulated kinase ( ERK ) , site directed mutagenesis was performed on the amino acid residues flanking the regulatory phosphorylation sites of ERK 1 . ^^^ These ERK 1 mutants were analyzed for the ability to act as a substrate for MEK 1 and MEK 2 . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| MAPK expression was determined in five human tumors and five normal tissues ( adjacent non neoplastic liver ) by Western blotting using specific antisera raised against four MAPK pathway intermediates : Erk 1 , Erk 2 ( extracellular signal regulated kinases ) , Mek 1 and Mek 2 ( mitogen activated protein kinase kinases ) . ^^^ There was a significant increase in Erk 1 , Erk 2 , Mek 1 and Mek 2 expression in particulate and cytosolic fractions prepared from tumor specimens as compared with the adjacent normal control tissues . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Mitogen activated protein ( MAP ) / ERK kinase ( MEK ) 1 and MEK 2 are the upstream activators of the MAP kinases , ERK 1 and ERK 2 . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Here we show that the corresponding sequences in human MEK 1 and MEK 2 are necessary and sufficient for the direct binding of the MAPKs ERK 1 and ERK 2 . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Our data demonstrate the requirement for Hsp 90 in estrogen induced activation of ERK 1 and ERK 2 by MEK 2 in the developing mouse cerebral cortex and also provide insight into alternative mechanisms by which estradiol may influence cytoplasmic and nuclear events in responsive neurons via the MAP kinase cascade . . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Western blot and immunohistochemistry were used to measure the expression of MEK 1 , MEK 2 and ERK 1 , ERK 2 protein . ^^^ RESULTS : MEK 2 and ERK 1 , ERK 2 protein levels were increased in breast carcinoma tissue compared with those in adjacent normal tissues ( t = 7 . 244 , 5 . 959 , 3 . 735 , P < 0 . 01 ) and benign tumors ( t = 2 . 206 , P < 0 . 05 ) . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| However , the reversibility of these cellular events , as well as the relative role of both MEK isoforms ( MEK 1 and MEK 2 ) and both ERK isoforms ( ERK 1 and ERK 2 ) during these processes , has not yet been investigated . ^^^ We now report that loss of constitutively active MEK 1 ( caMEK 1 ) and , thus , loss of active ERK1 / 2 in C7caMEK1 cells is associated with increased MEK 2 protein expression , reexpression of ERK 1 protein , and epithelial redifferentiation of these cells . ^^^ In these clones , increased MEK1 / 2 ERK1 / 2 phosphorylation , reduced MEK 2 protein expression , and loss of ERK 1 protein expression is associated with phenotypic alterations similar to those observed in transdifferentiated C7caMEK1 cells . ^^^ We conclude that in renal epithelial MDCK C 7 cells , stable epithelial to mesenchymal transition ( EMT ) is associated with loss of ERK 1 protein expression , reduced MEK 2 protein expression , and increased basal ERK 2 phosphorylation . ^^^ In contrast , loss of active MEK 1 ERK1 / 2 results in increased MEK 2 protein expression and reexpression of ERK 1 protein , concomitant with the restoration of epithelial phenotype and the ability to form cystic structures . . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Among the KRAS2 / BRAF wild type carcinomas , no mutations within pathway members MEK 1 , MEK 2 , ERK 1 , ERK 2 , RAP1B , or BAD were found . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Using in vitro assays , we show that platelet derived growth factor alpha ( PDGFA ) enhances medulloblastoma migration and increases downstream MAP2K1 ( MEK 1 ) , MAP2K2 ( MEK 2 ) , MAPK 1 ( p 42 MAPK ) and MAPK 3 ( p 44 MAPK ) phosphorylation in a dose dependent manner . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Mek 1 and Erk 1 depletion also caused cell cycle arrest at G 2 , suggesting that these enzymes are required for the G2 / M transition , whereas the loss of Mek 2 or Erk 2 caused arrest at G1 . . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| We now show that in double positive thymocytes Vav 1 is required for TCR induced activation of the ERK 1 and ERK 2 kinases via a pathway involving the Ras GTPase , and B Raf , MEK 1 , and MEK 2 kinases . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| NK ( 1 ) R mediated cell death was inhibited by a dominant negative form of arrestin 2 , Raf 1 , or Nur 77 , by MEK1 / 2 specific inhibitors , and by RNA interference directed against ERK 2 or MEK 2 but not ERK 1 or MEK 1 and against Nur 77 . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| The role of the extracellular signal regulated kinase ( ERK ) 1 and ERK 2 in the neutrophil chemotactic response remains to be identified since a previously used specific inhibitor of MEK 1 and MEK 2 , PD 98059 , that was used to provide evidence for a role of ERK 1 and ERK 2 in regulating chemotaxis , has recently been reported to also inhibit MEK 5 . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Mitogen activated protein ( MAP ) kinase kinases ( MKKs ) are dual specificity protein kinases which activate p42mapk and p44mapk by phosphorylation of regulatory tyrosine and threonine residues . cDNAs for two isotypes of MKK , MKK 1 and MKK 2 , have been isolated from several species . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Both cDNAs when expressed in COS cells displayed the ability to phosphorylate and activate p42mapk and p44mapk , both MKK 1 and MKK 2 were activated in vivo in response to serum , and both could be phosphorylated and activated by the 5 Raf protein in vitro . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| Transformation of immortalized rat embryo fibroblasts by a ts isolate of Moloney murine sarcoma virus ( Mo MuSVts 110 ) constitutively activates MAP kinases ( ERK 1 and ERK 2 ) and MAP kinase kinases ( MKK 1 and MKK 2 ) only at the permissive temperature when 5 mos kinase is present and active . ^^^ |
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| Interacting proteins: P36507 and P27361 |
Pubmed |
SVM Score :0.0 |
| The mitogen activated protein ( MAP ) kinase pathway , which includes extracellular signal regulated protein kinases 1 and 2 ( ERK 1 , ERK 2 ) and MAP kinase kinases 1 and 2 ( MKK 1 , MKK 2 ) , is well known to be required for cell cycle progression from G 1 to S phase , but its role in somatic cell mitosis has not been clearly established . ^^^ |
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