Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.87341197
Co immunoprecipitation studies showed decreased interactions of Bad with Akt and calcineurin in the cytosol and increased binding with Bcl xL in the mitochondrial fraction of hippocampal CA 1 , but not in the CA3 / dentate gyrus region . 0.87341197^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.63149438
Positive correlation between overexpression of phospho BAD with phosphorylated Akt at serine 473 but not threonine 308 in colorectal carcinoma . 0.63149438^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt phosphorylation of BAD couples survival signals to the cell intrinsic death machinery . ^^^ We show that growth factor activation of the PI3 ' K / Akt signaling pathway culminates in the phosphorylation of the BCL 2 family member BAD , thereby suppressing apoptosis and promoting cell survival . ^^^ Akt phosphorylates BAD in vitro and in vivo , and blocks the BAD induced death of primary neurons in a site specific manner . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Interleukin 3 induced phosphorylation of BAD through the protein kinase Akt . ^^^ Active , but not inactive , forms of Akt were found to phosphorylate BAD in vivo and in vitro at the same residues that are phosphorylated in response to IL 3 . ^^^ Thus , the proapoptotic function of BAD is regulated by the PI 3 kinase Akt pathway . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Dissociation of cytokine induced phosphorylation of Bad and activation of PKB / akt : involvement of MEK upstream of Bad phosphorylation . ^^^ PKB has also been implicated in the phosphorylation of Bad , potentially linking the survival effects of cytokines with the Bcl 2 family . ^^^ We have shown that granulocyte / macrophage colony stimulating factor ( GM CSF ) maintains survival in the absence of PI3K activity , and we now show that when PKB activation is also completely blocked , GM CSF is still able to stimulate phosphorylation of Bad . ^^^ IL 4 , unique among the cytokines in that it lacks the ability to activate the p21ras mitogen activated protein kinase ( MAPK ) cascade , was found to activate PKB and promote cell survival , but it did not stimulate Bad phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Inhibition of the anti apoptotic PI ( 3 ) K / Akt / Bad pathway by stress . ^^^ Down regulation of PI ( 3 ) K by ceramide results in inhibition of the kinase Akt and decreased phosphorylation of the death effector Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The kit receptor promotes cell survival via activation of PI 3 kinase and subsequent Akt mediated phosphorylation of Bad on Ser 136 . ^^^ SCF stimulation induces activation of phosphatidylinositol ( PI ) 3 kinase , which is required for SCF induced mitogenesis and cell survival , and for activation of the serine / threonine , we found that , in response to SCF Akt became activated and mediated phosphorylation of Bad , a pro apoptotic molecule , in a PI 3 kinase dependent manner . ^^^ Phosphorylation of Bad was restricted to Ser 112 and Ser 136 in vivo , but only the Akt phosphorylation sit Ser 136 was essential for SCF promoted cell survival . ^^^ Furthermore , Bad and Akt interacted and colocalized in intact cells . ^^^ A Kit / SCF R gain of function mutant that has increased mitogenic and PI 3 kinase activation potential , due to the absence of the two protein kinase C negative feedback phosphorylation site , enhanced both Akt activation and Bad phosphorylation and also resulted in increased cell survival . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We and others have described a linear survival signaling cascade used by insulinlike growth factor 1 ( IGF 1 ) that consists of the IGF 1 receptor , phosphoinositide 3 kinase ( PI 3 kinase ) , Akt , and Bad . ^^^ However , when IGF 1 receptors were overexpressed in a Rat 1 background ( RIG cells ) , an alternative pathway became apparent , in which survival mediated by IGF 1 was no longer sensitive to wortmannin or to overexpression of dominant negative Akt , even though Akt activation and Bad phosphorylation were still wortmannin sensitive . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The protection against apoptosis provided by growth factors in several cell lines is due to stimulation of the phosphatidylinositol 3 OH kinase ( PI ( 3 ) K ) pathway , which results in activation of protein kinase B ( PKB ; also known as c Akt and Rac ) and phosphorylation and sequestration to protein 14 3 3 of the proapoptotic Bcl 2 family member BAD . ^^^ Here we report that Ca2+ / calmodulin dependent protein kinase kinase ( CaM KK ) activates PKB directly , resulting in phosphorylation of BAD on serine residue 136 and the interaction of BAD with protein 14 3 3 . ^^^ These results identify a Ca2+ triggered signalling cascade in which CaM KK activates PKB , which in turn phosphorylates BAD and protects cells from apoptosis . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We show that the proapoptotic function of Bad in primary T cells is regulated by Akt kinase and that Bad overexpression enhances both cell cycle progression and interleukin 2 production after T cell activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Survival factors can suppress apoptosis in a transcription independent manner by activating the serine / threonine kinase Akt , which then phosphorylates and inactivates components of the apoptotic machinery , including BAD and Caspase 9 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , AAA PKB markedly inhibited the insulin induced phosphorylation of cotransfected BAD , demonstrating inhibition of the endogenous PKB / Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt activation increased Bad phosphorylation and promoted Pten / cell survival . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Here we show that FKHR , a human homologue of the DAF 16 transcription factor in Caenorhabditis elegans , is rapidly phosphorylated by human protein kinase Balpha ( PKBalpha ) at Thr 24 , Ser 256 , and Ser 319 in vitro and at a much faster rate than BAD , which is thought to be a physiological substrate for PKB . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Activation of phosphoinositide 3 kinases ( PI3Ks ) , their downstream target protein kinase B ( PKB ) , and phosphorylation of Bad have all been implicated in survival signaling in many systems . ^^^ To address this issue , we have used three different factor dependent hemopoietic cell lines , MC / 9 , BaF / 3 , and factor dependent ( FD ) 6 , which respond to a range of cytokines , to investigate the relationship between PI3K , PKB , and Bad activity with survival . ^^^ However , dual phosphorylation of the proapoptotic protein Bad was found not to correlate with PKB activation . ^^^ Insulin , which was the most potent inducer of PKB in FD 6 , induced barely detectable Bad phosphorylation . ^^^ These data demonstrate that , in hemopoietic cells , activation of PKB does not automatically confer a survival signal or result in phosphorylation of Bad , implying that other survival pathways must be involved . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
By contrast , suppression of mitochondrial Raf 1 enzymatic activity by expression of a mitochondria targeted Raf 1 dominant negative mutant rendered Akt expressing cells susceptible to apoptosis induced by growth factor deprivation and was accompanied by inhibition of BAD , but not mitogen activated protein kinase , phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Transient transfection with a PI3K dominant negative mutant or a dominant negative mutant of the serine threonine kinase Akt , the downstream target of PI3K and the enzyme that phosphorylates BAD , similarly potentiated the cytotoxicity of TNF . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
IL 3 treatment of responsive cells leads to the rapid and transient activation of Class IA phosphoinositide 3 kinases ( PI3Ks ) and the serine / threonine kinase Akt / protein kinase B ( PKB ) and phosphorylation of BAD . ^^^ Expression of Deltap 85 reduces IL 3 induced PKB phosphorylation and activation and phosphorylation of BAD dramatically , to levels seen in unstimulated cells . ^^^ These results show that Deltap 85 inhibits both PKB activity and BAD phosphorylation without significantly affecting levels of apoptosis , suggesting that there are targets other than PKB and BAD that can transmit survival signals in these cells . ^^^ Dissociation of apoptosis from proliferation , protein kinase B activation , and BAD phosphorylation in interleukin 3 mediated phosphoinositide 3 kinase signaling . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
BAD phosphorylation by Akt is an essential step for growth factor mediated inhibition of caspase activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
These results suggest that PKCdelta contributes to H ( 2 ) O ( 2 ) induced apoptosis by a mechanism independent of BAD and that PKCdelta is a target of PKB for the regulation of cell survival . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
During insulin stimulation , IRS 1 and IRS 2 strongly bound p85alpha / beta , which activated phosphatidylinositol ( PI ) 3 kinase , protein kinase B ( PKB ) / Akt , and p 70 ( s6k ) , and promoted the phosphorylation of BAD . ^^^ IRS 4 also promoted the activation of PKB / Akt and BAD phosphorylation during insulin stimulation ; however , it weakly bound or activated p 85 associated PI 3 kinase and failed to mediate the activation of p 70 ( s6k ) . ^^^ Thus , the activation of PKB / Akt and BAD phosphorylation might not be sufficient to inhibit the apoptosis of IL 3 deprived 32D ( IR ) cells unless p 85 associated PI 3 kinase or p 70 ( s6k ) are strongly activated . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
It has been shown that phosphorylation of BAD at Ser 136 is mediated by the serine / threonine protein kinase Akt 1 / PKB which is downstream of phosphatidylinositol 3 kinase ( PI3K ) . ^^^ Therefore , the PI3K Akt and Ras MAPK pathways converge at BAD by mediating phosphorylation of distinct serine residues . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In the presence of low concentrations of serum , enforced PTEN expression results in a marked increase in cellular apoptosis , a finding which is consistent with the capacity of PTEN to alter the phosphorylation , and presumably function , of the AKT , BAD , p70S6 kinase and GSK 3 alpha apoptosis regulators . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Taken together , our results show that Akt promotes cell survival by intervening in the apoptosis cascade before cytochrome c release and caspase activation via a mechanism that is distinct from Bad phosphorylation . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Stimulation of PKB activity protects cells from apoptosis by phosphorylation and inactivation of the pro apoptotic protein BAD . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt is more than just a Bad kinase . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
HGF induced rapid phosphorylation of Akt in HKC cells , which was immediately followed by phosphorylation and resultant inactivation of Bad , a pro apoptotic member of the Bcl 2 family . ^^^ Pretreatment of the HKC cells with 10 nM wortmannin completely abolished HGF induced phosphorylation of Akt and Bad , suggesting that this pathway is dependent on phosphoinositide ( PI ) 3 kinase . ^^^ HGF triggers Bad phosphorylation via the PI 3 kinase / Akt pathway , thereby inactivating this pro apoptotic protein , while simultaneously inducing expression of anti apoptotic Bcl xL . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Stimulation of beta 4 in A 431 cells resulted in a time dependent phosphorylation of Akt with a concomitant and parallel phosphorylation of Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The data demonstrate that c N Ras may play a functional role in the regulation of steady state phosphorylated Akt and serine 136 phosphorylated Bad ( Ser ( 136 ) pBad ) . ^^^ Elevated expression of either c Kirsten A Ras or c Kirsten B Ras did not reverse the apoptotic sensitivity of the N Ras knockout cells or result in increased levels of either phospho Akt or phospho Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
These results suggest that PDE3B , not PDE 4 , is a target of PKB and that activated PDE3B may regulate cAMP pools that modulate effects of PKB on thymidine incorporation and BAD phosphorylation in FDCP 2 cells . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
These LNAI cells show increased expression of integrin linked kinase , which is putatively responsible for AKT activation / Ser 473 phosphorylation , as well as for increased phosphorylation of the AKT target protein , BAD . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Rac 2 stimulates Akt activation affecting BAD / Bcl XL expression while mediating survival and actin function in primary mast cells . ^^^ Rac 2 ( / ) mast cells demonstrated a significant reduction in growth factor induced survival , which correlated with the lack of activation of Akt and significant changes in the expression of the Bcl 2 family members BAD and Bcl XL , in spite of a 3 fold induction of Rac 1 protein . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The antiapoptotic action of interleukin 3 ( IL 3 ) has been linked to a signaling pathway which inactivates the proapoptotic protein Bad by phosphorylation through kinases such as Akt and Raf . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt has been shown to mediate its apoptosis suppressive effects via phosphorylation of Bad or caspase 9 . ^^^ In endothelial cells , Akt seems to mediate its antiapoptotic effect , at least in part , via phosphorylation of caspase 9 rather than Bad . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
G CSF stimulation of cells expressing the G CSFR truncation mutant induces sustained activation of Akt and prolonged phosphorylation of the pro apoptotic protein Bad , resulting in enhanced cell survival . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt protects cells from apoptosis through phosphorylation of downstream targets such as the Bcl 2 family member , Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
GM CSF activated the PI 3 kinase / Akt pathway as determined by phosphorylation of Akt and BAD . ^^^ GM CSF dependent Akt and BAD phosphorylation was blocked by the PI 3 kinase inhibitor LY 294002 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The Akt / AKT1 clones also contained higher levels of phospho Bad protein than parental cells . ^^^ Further , the complexes between the endogenous proapoptotic protein , Bad , and the anti apoptotic protein , BC 1 XL were undetectable in Akt / AKT1 clones . ^^^ These results suggest that Akt / AKT1 expressed in these clones can phosphorylate Bad and prevent it from binding to Bcl XL . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
However insulin like growth factor 1 , a potent activator of PI3K and PKB does not increase the phosphorylation of Ser ( 136 ) in BAD transfected HEK 293 cells , and nor is the basal level of Ser ( 136 ) phosphorylation suppressed by inhibitors of PI3K . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Cardiotrophin 1 phosphorylates akt and BAD , and prolongs cell survival via a PI3K dependent pathway in cardiac myocytes . ^^^ CT 1 also phosphorylated the pro apoptotic factor , BAD , and the BAD phosphorylation was inhibited by LY 294002 , suggesting that phosphorylation of BAD is one of the key events by which the PI3K / Akt pathway mediates CT 1 induced survival signaling . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The PRK 2 C terminal fragment strongly inhibits the Akt mediated phosphorylation of BAD , a pro apoptotic Bcl 2 family protein , and blocks the anti apoptotic activities of Akt in vivo . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt inhibits apoptosis by phosphorylating Bad , thus promoting its binding to and blockade of the activity of the cell survival factor Bcl 10 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Lipoarabinomannan from Mycobacterium tuberculosis promotes macrophage survival by phosphorylating Bad through a phosphatidylinositol 3 kinase / Akt pathway . ^^^ Since phosphorylation of Bad occurred at serine 136 , the target of the serine / threonine kinase Akt , the effect of LAM on Akt kinase activity was tested . ^^^ The phosphorylation of Bad by man LAM was abrogated in cells transfected with a kinase dead mutant of Akt . ^^^ These results establish that LAM mediated Bad phosphorylation occurs in a PI 3K / Akt dependent manner . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The first included Akt and Bad , leading to an inhibition of apoptosis ; the second included the mitogen activated protein kinase ( MAPK ) , resulting in proliferation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We showed that CISK acts downstream of the PI 3 kinase cascade in vivo and may function in parallel to Akt by phosphorylating Bad and the transcription factor FKHRL 1 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Inhibition of BAD phosphorylation either at serine 112 via extracellular signal regulated protein kinase cascade or at serine 136 via Akt cascade sensitizes human ovarian cancer cells to cisplatin . ^^^ We studied the roles of the phosphatidylinositol 3 kinase ( PI 3K ) protein kinase B / Akt BAD cascade in both cisplatin resistant Caov 3 and sensitive A 2780 human ovarian cancer cell lines . ^^^ Cisplatin did not stimulate the phosphorylation of BAD Ser 136 , but did stimulate the phosphorylation of BAD Ser 112 in cells expressing a dominant negative Akt , suggesting that BAD Ser 136 but not Ser 112 was phosphorylated by Akt . ^^^ Our findings suggest that cisplatin induced DNA damage causes the phosphorylation of both BAD Ser 112 via an extracellular signal regulated protein kinase ( ERK ) cascade and BAD Ser 136 via a PI 3K protein kinase B / Akt cascade and that inhibition of either of these cascades sensitizes ovarian cancer cells to cisplatin . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
RIN Arg 972 cells exhibited a marked impairment in the sequential activation of PI 3 kinase , Akt , and BAD as compared with RI N WT . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Insulin strongly activated both PI3K and the putative downstream effector AKT : Moreover , a proapoptotic protein , BAD : , was significantly phosphorylated and inactivated by insulin through PI3K . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Coordinate inactivation of cell survival pathways , including cleavage of focal adhesion kinase , decreased expression of protein kinase B , and reduced phosphorylation of the pro apoptotic protein , Bad , also characterized the signaling pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The antiapoptotic protein Bad , a downstream target of PKB / Akt , is also phosphorylated . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
AKT functions , in part , to promote cell survival by phosphorylating the BCL 2 family member BAD and the cell death pathway enzyme , caspase 9 . ^^^ UV also stimulated phosphorylation of the downstream AKT effectors , S 6 kinase and BAD . ^^^ Western blot analysis revealed that UV induced phosphorylation of BAD at Ser 112 , a site known to be phosphorylated by AKT . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In vivo PDGF beta receptor activation in the dorsocaudal brainstem of the rat prevents hypoxia induced apoptosis via activation of Akt and BAD . ^^^ Since hypoxia does not induce apoptosis in the caudal brainstem , PDGFR could underlie such protective mechanism via a PI 3 kinase dependent phosphorylation of both Akt and BAD pathways . ^^^ We conclude that PDGFR dependent in vivo activation of both Akt and BAD during hypoxia prevents induction of apoptosis , and may contribute to the increased hypoxic tolerance of brainstem neurons . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Consistent with the capacity of RSK and Akt to mediate a cell survival signal , in part through phosphorylation of the pro apoptotic protein BAD , TPCK reduced BAD phosphorylation and led to cell death in interleukin 3 dependent 32D cells . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phosphorylation of Akt at serine 473 and Bad at serine 136 were induced by HBx , which were specifically blocked by wortmannin and dominant negative mutants of Akt and Bad , respectively . ^^^ The results indicate that , unlike other DNA tumor viruses that block apoptosis by inactivating p 53 , the hepatitis B virus achieves protection from apoptotic death through a HBx PI3K Akt Bad pathway and by inactivating caspase 3 activity that is at least partially p 53 independent in liver cells . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
STR inhibited the phosphorylation of Bad , whereas Bad phosphorylation was preserved in cells that overexpress Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The expression of phospho Bad was greater in negative cases , suggesting that Bad might be a target for AKT : The present study demonstrates the phosphorylation of Akt accompanied by the loss of PTEN in clinical specimens of endometrial carcinomas . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Neuregulin signaling through a PI3K / Akt / Bad pathway in Schwann cell survival . beta Neuregulin ( betaNRG ) is a potent Schwann cell survival factor that binds to and activates a heterodimeric ErbB2 / ErbB3 receptor complex . ^^^ The activation of Akt and MAPK in parallel pathways downstream from PI3K resulted in the phosphorylation of Bad at different serine residues . ^^^ PI3K inhibitors that blocked NRG mediated rescue also blocked the phosphorylation of Akt , MAPK , and Bad . ^^^ Conversely , ectopic expression of wild type Akt not only enhanced Bad phosphorylation but also enhanced autocrine and NRG mediated Schwann cell survival . ^^^ Taken together , these results demonstrate that NRG receptor signaling through a PI3K / Akt / Bad pathway functions in Schwann cell survival . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Adhesion of integrin receptors to extracellular matrix activates a survival signaling pathway in epithelial cells where Akt phosphorylates and blocks the activity of proapoptotic proteins such as the BCL 2 family member Bad , the forkhead transcription factor FKHRL 1 , and caspase 9 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In addition , relative expression levels of six Bcl 2 homologs ( Bcl 2 , Bcl 10 ( L ) , Mcl 1 , Bax , Bak , and Bad ) and activation levels of Fak , Erk 2 , and Akt were analyzed . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
On the contrary , the significant decrease of Shc phosphorylation , Grb 2 recruitment and the reduced phosphorylation level of Akt Thr 308 and Akt substrates FKHR and Bad detected in transgenic glands show that activation of the Shc and the Akt pathways require intact cell ECM interactions . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
When co expressed with Akt , wild type 14 3 3 could reduce the ability of Bad to cause death , however 14 3 3zetaK49E , which can not bind Bad , failed to inhibit Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Gas 6 anti apoptotic signaling was previously shown to require functional PI3K and Akt and to involve Bad phosphorylation in serum starved NIH 3T3 cells . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Treatment of endothelial cells with HDL induced activation of the protein kinase Akt , an ubiquitous transducer of anti apoptotic signals , and led to phosphorylation of BAD , a major Akt substrate . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Immunoblotting demonstrated a decrease in the phosphorylation of Akt and Bad , and also Bcl 2 levels were reduced in aortic banded cyclosporine treated animals at 7 days compared with aortic banded vehicle treated animals . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Thus , our findings demonstrate that the constitutive activation of Akt 1 regulates macrophage survival through Mcl 1 , which is independent of caspases , NF kappaB , or Bad . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
C5b 9 terminal complement complex protects oligodendrocytes from death by regulating Bad through phosphatidylinositol 3 kinase / Akt pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Since this down regulation of Akt was prevented by CsA , the results suggested a link between dephosphorylaltion of Bad , and cell death . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
IGF 1 stimulation was followed by a PI3K dependent phosphorylation of AKT and BAD and an MEK 1 dependent phosphorylation of extracellular signal regulated kinase ( ERK ) 1 and ERK 2 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Analysis of signal transduction pathways revealed that NGF withdrawal inhibits the PI 3 K , protein kinase B ( AKT ) , Bad survival pathway and activates c Jun kinase ( JNK ) whereas ERKs and p 38 mitogen activated protein kinase ( MAPK ) are not affected . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Cisplatin induced Akt / PKB activation was associated with Bad phosphorylation , suggesting induction of a cell survival signal mediated by the Bcl 2 family member , Bad . ^^^ Wortmannin or LY 294002 , two structurally dissimilar inhibitors of phosphatidylinositol 3 ' kinase ( PI 3 kinase ) , abolished both cisplatin induced Akt phosphorylation and Bad phosphorylation , and promoted cisplatin induced early and accelerated activation of caspase 3 and caspase 9 , but not of caspase 8 and caspase 1 , indicating that inhibition of the Akt / PKB phosphorylation pathway enhances the mitochondrial dependent activation of caspases . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We studied whether pro survival Akt was activated after transient focal cerebral ischemia and whether it inhibited pro apoptotic Bad . ^^^ In contrast to Akt , no Bad phosphorylation ( serine 136 ) was detected after ischemia . ^^^ Therefore , injury spontaneously activated Akt , but this did not suppress Bad signalling . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Finally , downstream prosurvival targets of Akt including p70s6 kinase and BAD were modulated by insulin . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
After phosphorylation , activated Akt inactivates other apoptogenic factors , such as Bad or caspase 9 , thereby inhibiting cell death . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The serine threonine kinase Akt exerts its anti apoptotic effects through several downstream targets , including the pro apoptotic Bc 1 2 family member Bad , Forkhead transcription factors , and the cyclic AMP response element binding protein ( CREB ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The delayed involution phenotype corresponded to increased phosphorylation of Thr 308 in AKT 1 and Ser 136 in BAD , but not phosphorylation of Ser 21 in GSK 3alpha . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , there was phosphorylation of two important prosurvival PKB targets , 1 kappaBalpha ( Ser 32 ) and Bad ( Ser 136 ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
PI 3 kinase Akt pathway activation by C5b 9 induced , within 15 min , a 6 . 34 + / 1 . 2 fold increase in BAD phosphorylation at Ser 136 , but not at Ser 112 . ^^^ Our data indicated that sublytic C5b 9 rescued Schwann cell from apoptosis via activation of PI 3 kinase Akt , BAD phosphorylation on Ser 136 and increased expression of Bcl 10 ( L ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
IL 6 also induced phosphorylation of downstream targets of Akt , including Bad , GSK 3beta , and FKHR , confirming Akt activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
PI 3 K downstream signaling events were evaluated by measuring the expression of cytosolic total and phosphorylated Akt and Bad proteins by Western blot analyses , and caspase 9 activity . ^^^ Brief stimulation of neutrophils with C5a induced phosphorylation of Akt and Bad proteins through a PI 3 K dependent pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Once activated , Akt can prevent or delay apoptosis by phosphorylation dependent inhibition or activation of multiple signaling molecules involved in apoptosis , such as BAD , caspase 9 , GSK 3 , and NF kappaB and forkhead family transcription factors . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , the known downstream modulators of the Akt / PI3K cell survival pathway , Bcl 10 ( L ) , and BAD proteins showed decreased expression after I3C treatment . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The rescue of cells from CsA induced apoptosis by A 23187 was correlated with AKT activation , BAD phosphorylation , and caspase 3 inactivation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Moreover , we found that Tat enhanced phosphorylation of AKT and BAD proteins . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
These findings suggest that modulation of MAP kinase and Akt expression , Bcl xL , Bcl xs , Bcl 2 and Bad proteins by finasteride may be , in part , responsible for the anti proliferative and apoptotic effect of this drug seen clinically and in animal models . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
With the same in vitro assay , we also demonstrate interactions of protein kinase PKB with substrate Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Inhibition of phosphorylation of BAD and Raf 1 by Akt sensitizes human ovarian cancer cells to paclitaxel . ^^^ We studied the roles of the phosphatidylinositol 3 kinase ( PI 3K ) Akt BAD cascade , ERK BAD cascade , and Akt Raf 1 cascade in the paclitaxel resistant SW 626 human ovarian cancer cell line , which lacks functional p 53 . ^^^ The phosphorylation of BAD at Ser 136 was blocked by treatment with wortmannin or cotransfection with the dominant negative Akt . ^^^ Our findings suggest that paclitaxel induces the phosphorylation of BAD Ser 112 via the ERK cascade , and the phosphorylation of both BAD Ser 136 and Raf 1 Ser 259 via the PI 3K Akt cascade , and that inhibition of either of these cascades sensitizes ovarian cancer cells to paclitaxel . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Downstream molecules of Akt , including NF kappaB , Fas ligand , and BAD were examined , among which phosphorylation of BAD by Axl Gas 6 signaling is associated with the anti apoptotic activity of Akt in our study . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
ICAM 2 induced tyrosine phosphorylation of ezrin and PI3K kinase membrane translocation , resulting in phosphatidylinositol 3 , 4 , 5 production , PDK 1 and AKT activation , and subsequent phosphorylation of AKT targets BAD , GSK 3 , and FKHR . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Cellular N Ras provides a steady state antiapoptotic signal , at least partially through the regulation of phosphorylated Akt and Bad levels . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Chemical inhibition of Akt phosphorylation with SB 203580 partially blocked the protective effect of cAMP . cAMP resulted in wortmannin independent phosphorylation of BAD and was associated with translocation of BAD from the mitochondria to the cytosol . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Consistent with this observation and the antiapoptotic properties of NS5A , we also detected enhanced tyrosine phosphorylation of the downstream AKT protein kinase and increased serine phosphorylation of BAD , a proapoptotic factor and an AKT substrate , in the presence of NS5A . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Concomitant activation of the insulin like growth factor 1 receptor , Akt , and extracellular signal regulated protein kinase 1 and 2 as well as increased phosphorylation of Bad also were unique to the hippocampus of APP ( Sw ) mice . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Western blot analysis revealed that Cl F araA induced a dose and time dependent downregulation of Bcl 10 ( L ) and Mcl 1 proteins , and a dose and time dependent dephosphorylation of Akt and its downstream effectors ( Bad , FKHRL 1 ) , particularly in vivo . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phosphorylation of Akt and Bad was stimulated in areas where IGF 1 was increased , with no change in MAPK or glycogen synthase kinase 3beta . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In contrast , the primarily uninjured cortex exhibited increased phosphorylation of Akt ( protein kinase B ) , which may phosphorylate and inhibit BAD , and no altered binding of BAD to BCL Xl . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Treatment of cells with PMA or L PGDS decreased phosphatidylinositol 3 kinase ( PI 3 K ) activity and concomitantly inhibited protein kinase B ( PKB / Akt ) phosphorylation , which led to the hypophosphorylation and activation of Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Activation of Akt maintains Bad phosphorylation and prevents its binding to mitochondrial targets , decreases caspase 9 activity , and prevents the translocation of forkhead transcription factors ( FKHR ) . ^^^ Akt , Bad , FKHR phosphorylation , and mitochondrial cytochrome release were analyzed by Western blots . ^^^ Akt activation ; increase in islet viability ; and decrease in Bad phosphorylation , cytochrome release , caspase 9 activation , and translocation of FKHR were observed after simvastatin treatment , effects reversed by LY 294002 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In addition , neuronal cultures generated from ethanol exposed pups had reduced levels of insulin stimulated Akt , GSK 3beta , and BAD phosphorylation , and increased levels of non phosphorylated ( activated ) GSK 3beta and BAD protein expression . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
N cadherin homophilic ligation initiates phosphatidylinositol 3 kinase dependent activation of Akt resulting in Akt phosphorylation of Bad on serine 136 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Ganglioside depletion promotes survival of the human keratinocyte derived SCC 12 cell line through upregulated phosphorylation of beta 1 integrin , and increased phosphorylation and activity of integrin linked kinase , protein kinase B / Akt , and Bad , with resultant inhibition of caspase 9 activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Erythropoietin fosters both intrinsic and extrinsic neuronal protection through modulation of microglia , Akt 1 , Bad , and caspase mediated pathways . 1 . ^^^ Neuronal injury was evaluated by trypan blue , DNA fragmentation , phosphatidylserine ( PS ) exposure , Akt 1 phosphorylation , Bad phosphorylation , mitochondrial membrane potential , and cysteine protease activity . ^^^ EPO increases Akt 1 activity , phosphorylates Bad , and maintains neuronal nuclear DNA integrity through the downstream modulation of mitochrondrial membrane potential , cytochrome c release , and caspase 1 , 3 , and 8 like activities . 6 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Ceramide induced neuronal apoptosis is associated with dephosphorylation of Akt , BAD , FKHR , GSK 3beta , and induction of the mitochondrial dependent intrinsic caspase pathway . ^^^ We show that ceramide treatment initiates a cascade of biochemical alterations associated with cell death : earliest signal transduction changes involve Akt dephosphorylation and inactivation followed by dephosphorylation of proapoptotic regulators such as BAD ( proapoptotic Bcl 2 family member ) , Forkhead family transcription factors , glycogen synthase kinase 3 beta , mitochondrial depolarization and permeabilization , release of cytochrome c into the cytosol , and caspase 3 activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In MM cell lines and patient MM cells , SDF 1alpha induces phosphorylation of p42 / 44 mitogen activated protein kinase , as well as Akt and its downstream target Bad , and also activates nuclear factor kappaB . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Western blot analysis using phosphorylation specific antibodies to Akt and MAP kinase revealed markedly elevated activation in adherent SCLC sublines , paralleled by increased levels of phosphorylated Bad protein and activated NF kappaB . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We next examined the apoptosis related genes in the context of a molecular interaction map and found expression differences in the direction `` expected ' ' on the basis of the apoptosis resistance of RC0 . 1 for BAD , caspase 6 , and genes that signal via the Akt pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Moreover , TIMP 1 enhances specific phosphorylation of both Akt and Bad ( Bcl 2 / Bcl 10 ( L ) antagonist , causing cell death ) in a PI 3 kinase dependent manner and , besides , controls the level of the anti apoptotic protein Bcl 10 ( L ) . ^^^ We conclude that TIMP 1 induces haematopoietic cell survival via the JAK2 / PI 3 kinase / Akt / Bad pathway . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Cyclosporin A interfered with Taxol ( 0 . 1 microM ) induced AKT activation and BAD phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NF kappaB , the c jun N terminal kinase c Jun pathway , p 53 , E2F1 , and other transcription factors are probably all involved in regulating the expression of BH 3 only proteins after brain ischaemia , and mitochondrial translocation of Bad from sequestering cytosolic proteins is promoted by inactivation of the serine threonine kinase , Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
IL 7 also blocked dexamethasone triggered dephosphorylation of the serine threonine kinase Akt / PKB and its target , the Ser ( 136 ) residue in Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In addition , ethanol exposure significantly reduced the levels of total phosphoinositol 3 kinase , Akt kinase , phospho BAD ( inactive ) , and glyceraldehyde 3 phosphate dehydrogenase and increased the levels of glycogen synthase kinase 3 activity , activated BAD , phosphatase and tensin homolog deleted in chromosome 10 ( PTEN ) protein , and PTEN phosphatase activity in cerebellar tissue . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Bad , an Akt substrate involved in cell survival , appeared modified in its phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
This anti apoptotic effect was dependent on the activation of phosphatidylinositol 3 kinase and associated with the activation of Akt and the phosphorylation of Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phosphorylation of Bad ( pBad ) , a substrate of Akt , was also increased in the tumor tissues in association with activation of Akt , and the T / N ratio for pAkt positively correlated to the T / N ratio for pBad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
After phosphorylation , activated Akt inactivates BAD or caspase 9 or other apoptogenic components , thereby inhibiting cell death . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We have found that TCR cross linking by Con A and not by alpha CD 3 epsilon decreases the gene and protein expression of the pro apoptotic molecule , Bad ; and that Con A is capable of the activation of the survival signalling pathway including protein kinase B ( Akt / PKB ) independently of phosphatidyl inositol kinase ( PI3K ) . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The PI 3 kinase Akt pathway promotes mesangial cell survival and inhibits apoptosis in vitro via NF kappa B and Bad . ^^^ While PDGF activated NF kappa B and phosphorylated Bad , these reactions were inhibited by overexpression of the dominant negative mutant of Akt as well as the PI 3 kinase inhibitors . ^^^ These data indicate , firstly , that Akt is phosphorylated by PDGF , and secondly , that the activated Akt prevents apoptotic changes via activation of NF kappa B and phosphorylation of Bad in mesangial cells . ^^^ This study investigated whether it is Bad phosphorylation or NF kappa B activation that provides the anti apoptotic effects of Akt , and the data suggested that NF kappa B is probably the principal contributor to the downstream activation of the PI 3 kinase Akt pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Both SNAP and 8 Br cGMP induced endogenous Akt activation and Bad phosphorylation , resulting in the inhibition of Bad translocation to mitochondria ; these effects were inhibited by KT 5823 and the phosphatidylinositol 3 kinase ( PI3K ) inhibitors LY 294002 and Wortmannin . ^^^ Our data suggest that the NO / cGMP pathway suppresses 6 OHDA induced PC 12 cell apoptosis by suppressing the mitochondrial apoptosis signal via PKG / PI3K / Akt dependent Bad phosphorylation . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
To let the fluorescent indicators behave as endothelial nitric oxide synthase and Bad , which are endogenous Akt / PKB substrates , they were fused with the Golgi target domain and mitochondria target domain , respectively . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Analysis of multiple genes involving the apoptotic pathway reveals inhibition of Akt activity without affecting the steady state levels of Akt protein expression and the down regulation of proapoptotic gene BAD expression . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Moreover , AM increased Akt and Bad phosphorylation and Bcl 2 levels , but decreased caspase 3 activation . ^^^ These results indicate that AM protects against myocardial infarction , arrhythmia , and apoptosis in I / R injury via suppression of oxidative stress induced Bax and p 38 MAPK phosphorylation and activation of the Akt Bad Bcl 2 signaling pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In extending these findings , we now report a marked decrease in protein kinase C ( PKC ) isoforms , reduced Akt serine / threonine kinase activity , Bcl 2 associated death promoter ( BAD ) phosphorylation and enhanced p 38 mitogen activated protein kinase ( MAPK ) and caspase 9 and 3 activation , 12 h after addition of both 5 micro m Abeta and 5 micro m Fe2+ . ^^^ Abeta alone , increased PKC isoform levels between three and four fold after 12 h , enhanced Akt activity approximately eight fold and Ser 136 BAD phosphorylation two fold , suggesting that by itself is not toxic . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Serum activation of Akt as well as BAD phosphorylation by Akt showed no difference in 293 cells transfected with PED / PEA 15 and in untransfected cells ( which express no endogenous PED / PEA 15 ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Quercetin induced potent inhibition of both Akt / PKB and ERK phosphorylation , resulting in reduced phosphorylation of BAD and a strong activation of caspase 3 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
One such target is Bad ; its phosphorylation and inactivation by 14 3 3 protects podocytes against detachment induced cell death , suggesting that the nephrin CD2AP mediated AKT activity can regulate complex biological programs . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Selective involvement of the PI3K / PKB / bad pathway in retinal cell death . ^^^ The phosphoinositide 3 kinase ( PI3K ) / protein kinase B ( PKB ) / Bad signal transduction pathway is engaged in the control of apoptosis in many different cell types , particularly through phosphorylation of the Bcl 2 family protein Bad . ^^^ PKB is constitutively phosphorylated in retinal tissue in vitro , whereas Bad was dephosphorylated both in Ser 112 and Ser 136 . ^^^ The data suggest that Bad may be engaged in survival pathways in the immature retina , but pathways other than PI3K / PKB / Bad , and phosphorylation sites other than Ser 112 and Ser 136 in the Bad protein control cell survival in retinal tissue . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In addition , activation of other downstream components including PI3K , Akt , GSK3beta , Bad , and p70S6 kinase was studied . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Importantly , pro apoptotic activity of wild type BAD ( BADwt ) was strongly suppressed by co transfection with constitutively active Akt ( CA Akt ) cDNA , whereas that of BADD119G was not . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The authors examined the effect of SS and CS on inhibition of apoptosis and phosphorylation of Akt and its downstream target Bad in bovine aortic ECs in vitro . ^^^ These results suggest that hemodynamic forces suppress apoptosis in ECs via phosphorylation of Akt and that SS and CS differentially activate the downstream phosphorylation of Bad , possibly by stimulating an alternate pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
On the other hand , overexpression of MelCAM activated endogenous AKT and inhibited proapoptotic protein BAD in melanoma cells , leading to increased survival under stress conditions . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The antiapoptotic function of the IGF 1 receptor was associated with increased Akt kinase activity and increased expression of phosphorylated Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Apoptosis effected by TNF alpha alone was suppressed in the presence of ATA , and this effect appeared essentially characterized by : ( 1 ) phosphorylation of phosphatidylinositol 3 kinase ( PI 3K ) , influencing in turn protein kinase B / Akt ( Akt ) and Bad phosphorylation ; ( 2 ) nuclear translocation of the nuclear factor kappa B ( NF kappaB ) and ( 3 ) nuclear translocation of protein kinase C zed ( PKCzeta ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Moreover , EGCG induced Erk phosphorylation was found to be critical for the phosphorylation of Ser 112 in Bad protein , and the EGCG induced activation of the Akt pathway was found to be involved in the phosphorylation of Ser 136 . ^^^ In conclusion , this study demonstrates that EGCG promotes keratinocyte survival and inhibits the UV induced apoptosis via two mechanisms : by phosphorylating Ser 112 and Ser 136 of Bad protein through Erk and Akt pathways , respectively , and by increasing the Bcl 2 to Bax ratio . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Here we report that activin A alone activates IkappaB alpha , and stimulates nuclear translocation of NFkappaB and receptor activator of nuclear factor kappaB ( RANK ) expression for osteoclastogenesis , but not Akt / PKB survival signal transduction including BAD and mammalian target of rapamycin ( mTOR ) for survival in osteoclast precursors in vitro . ^^^ Activin A alone failed to activate Akt , BAD , and mTOR by immunoblotting , and it also failed to prevent apoptosis in osteoclast precursors . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
SCH 66336 suppressed protein kinase B / Akt activity as well as the phosphorylation of the Akt substrates glycogen synthase kinase ( GSK ) 3 beta , forkhead transcription factor , and BAD . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Cotransfection of BAD with constitutively active Akt cDNA strongly inhibited this change . ^^^ In contrast , the increase of unphosphorylated BAD was not significantly inhibited by several phosphatase inhibitors or cotransfection with a dominant negative calcineurin cDNA , implying that the increase may be mainly due to a decrease of serine kinase activity , such as that of Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phosphorylation of Bad induced by BMK 1 activation was independent of Akt , PKA , or p90RSK kinase activity . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The EGCG dependent reduction in ERK and AKT activity is associated with reduced phosphorylation of downstream substrates , including p90RSK , FKHR , and BAD . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The peptides inhibit the in vitro phosphorylation of a biotinylated Bad peptide by Akt with potency up to 100 nM . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We also show that Src directed activation of insulin receptor substrate 1 , phosphatidylinositol 3 kinase ( PI3K ) , Akt , and Bad phosphorylation conform a substantial component of the survival route because pharmacological inhibition of PI3K and Akt canceled the antiapoptotic effects of NO . ^^^ By contrast , Akt and Bad activation are partially dependent on PKG activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
For example , the inhibition of the PI 3 K / Akt 1 pathway down regulated Bcl XL , Mcl 1 , and Bad , while at the same time up regulating Bax , whereas the inhibition of Fak up regulated both Bax and Bak , down regulated Bad , and did not affect the other Bcl 2 homologs analyzed . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Decreased phosphorylated Akt by LY 294002 treatment led to a down regulation of Mcl 2 and phosphorylated Bad proteins , which are anti apoptotic factors and belong to the Bcl 2 family . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The substrates of Akt / PKB , which promote the inhibition of apoptosis after being phosphorylated by Akt , include the Forkhead transcription factors and the Bcl 2 family member Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The animals were killed 11 weeks after the DMBA application , and the levels of IGF 1 , IGF 1 receptor ( IGF IR ) , Akt , Bcl 2 and Bad in tumorous and nontumorous breast tissue samples were measured by Western blot analysis . ^^^ The samples collected from tumor tissues showed lower levels of IGF 1 , Akt , Bcl 2 , Bad , and IGF IR in comparison with nontumorous tissues . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Downstream targets of PI3K AKT include the proapoptotic protein BAD , caspase 9 , NF kappaB , and Forkhead . ^^^ In this study , we tested the hypothesis that the inhibition of PI3K by LY 294002 results in the dephosphorylation of AKT and BAD , and thus promote leukemia cell apoptosis . ^^^ In MO7E cells , LY 294002 reduced AKT kinase activity , induced dephosphorylation of AKT and BAD , and increased apoptosis . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , Akt prevented release of cytochrome c and cleavage of Bad in DDN treated HL 60 cells . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt negatively regulates apoptotic pathways at a premitochondrial level through phosphorylation and modulation of proteins such as Bad , Forkhead proteins , and GSK 3beta . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Estradiol abrogates apoptosis in breast cancer cells through inactivation of BAD : Ras dependent nongenomic pathways requiring signaling through ERK and Akt . ^^^ BAD S112A / S136A , which lacks phosphorylation sites for p 90 ( RSK 1 ) and Akt , was not phosphorylated in response to E ( 2 ) in vitro ( . ) E ( 2 ) treatment rapidly activated phosphatidylinositol 3 kinase ( PI 3K ) / Akt and p 90 ( RSK 1 ) to an extent similar to insulin like growth factor 1 treatment . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The integration of these intracellular signals at the level of Akt and its kinase activity , regulates the phosphorylation of its several downstream effectors , such as NF kappa B , mTOR , Forkhead , Bad , GSK 3 and MDM 2 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The PI3K / AKT pathway and induction of phosphorylation of BAD . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Treatment of endothelial cells with Andro induced activation of the protein kinase Akt , an anti apoptotic signal , and phosphorylation of BAD , a down stream target of Akt . ^^^ In conclusion , Andro exerts its anti apoptotic potential via activation of the Akt BAD pathway in HUVECs and thus may represent a candidate of therapeutic agent for atherosclerosis . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
By selectively governing the activity of specific downstream substrates that include GSK 3beta , Bad , and beta catenin , Wnt and Akt serve to foster neuronal and vascular survival and block the induction of programmed cell death . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , forced expression of Cyr 61 in the glioma cells activated phosphatidylinositol 3 ' kinase pathway , resulting in prominent phosphorylation of Akt and the antiapoptotic protein Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Here we report that nicotine potently induces Bad phosphorylation at Ser 112 , Ser 136 , and Ser 155 in a mechanism involving activation of MAPKs ERK1 / 2 , PI3K / AKT , and PKA in human lung cancer cells . ^^^ The fact that beta adrenergic receptor inhibitor ( propranolol ) blocks nicotine induced activation of ERK1 / 2 , AKT , PKA , Bad phosphorylation , and cell survival suggests that nicotine induced Bad phosphorylation may occur through the upstream beta adrenergic receptors . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The induction of cytochrome c release may have been due to dephosphorylation of Akt and Bad , which were cooperatively induced by CHX and LPS . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phosphorylation of Akt at serine 473 and its downstream molecular Bad at serine 136 was induced by HAPO , but was blocked by two PI3K inhibitors , LY 294002 and wortmannin . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phospho Akt / phospho Bad double staining revealed colocalization . ^^^ The data support prominent roles for Bad phosphorylation in phospho Akt ' s reduction of cyt c apoptosis , and possible apoptotic roles at mitochondrial targets of Bad . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt exerts its antiapoptotic function by its ability to phosphorylate many key components of the cellular apoptotic regulatory circuit , such as BAD , MDM 2 , FOXO Forkhead transcription factors , and PED ? ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
It also was observed that IC 101 caused a concentration dependent dephosphorylation of Akt and Bad without affecting phosphatidylinositol 3 kinase , an upstream molecule of Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
PC also induced phosphorylation of Akt and BAD . ^^^ CONCLUSIONS : Our data demonstrated that in adult cardiomyocytes , the antiapoptotic effect of PC against H / R requires Akt signaling leading to phosphorylation of BAD , inhibition of cytochrome c release , and prevention of caspase activation . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt has direct effects on the apoptosis machinery , for example targeting the pro apoptotic Bcl 2 related protein , BAD . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The data show further that endogenous protein kinase A is a primary catalyst of cellular Bad phosphorylation in response to Rac activation , while Akt is not involved . ^^^ Rac 1 inhibits apoptosis in human lymphoma cells by stimulating Bad phosphorylation on Ser 75 . ^^^ In this report , constitutively active Rac 1 is shown to stimulate the phosphorylation of the Bcl 2 family member Bad , thereby suppressing drug induced caspase activation and apoptosis in human lymphoma cells . ^^^ Rac 1 activation leads to human Bad phosphorylation specifically at serine 75 ( corresponding to murine serine 112 ) both in vivo and in vitro . ^^^ Inhibition of constitutive and activated Rac 1 induced Bad phosphorylation by a cell permeable competitive peptide inhibitor representing this Bad phosphorylation site sensitizes lymphoma cells to drug induced apoptosis . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Restoration of Akt activity by orthovanadate treatment correlated positively with increased phosphorylation of glycogen synthase kinase 3beta and Bad in cardiomyocytes . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Serum bioactive lysophospholipids prevent TRAIL induced apoptosis via PI3K / Akt dependent cFLIP expression and Bad phosphorylation . ^^^ LPA increased phosphatidylinositol 3 kinase ( PI3K ) dependent Akt activation , cellular FLICE inhibitory protein ( cFLIP ) expression , and Bad phosphorylation , resulting in inhibition of caspase 8 activation and Bad translocation to mitochondria . ^^^ The antiapoptotic effect of LPA was abrogated by PI3K inhibitor , transfection with dominant negative Akt , and specific downregulation of cFLIP expression using siRNA and further increased by siRNA mediated suppression of Bad expression . ^^^ Our results indicate that albumin bound LPA and S1P prevent TRAIL induced apoptosis by upregulation of cFLIP expression and in part by Bad phosphorylation , through the activation of PI3K / Akt pathway . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The phosphorylation of FKHR , FKHRL , and Bcl 2 family member Bad was markedly increased in response to oxidant injury , and this increase was associated with elevated levels of basal phosphorylation of Akt / protein kinase B . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The cell survival Akt signaling pathway was activated in FTY 720 groups by phosphorylation of Glycogen Synthase Kinase 3beta , Bad and Forkhead Transcription Factor at 6 and 24 h after liver transplantation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Here , we demonstrate that PKCdelta activity and mitochondrial translocation at the onset of reperfusion mediates apoptosis by facilitating the accumulation and dephosphorylation of the pro apoptotic BAD ( Bcl 2 associated death promoter ) , dephosphorylation of Akt , cytochrome c release , PARP ( poly ( ADP ribose ) polymerase ) cleavage , and DNA laddering . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Resveratrol induced expression of Bcl 2 and caused its phosphorylation along with phosphorylation of cAMP response element binding protein ( CREB ) , Akt , and Bad . ^^^ CPT blocked phosphorylation of Akt and Bad without affecting CREB , whereas MRS 1191 blocked phosphorylation of all compounds , including CREB . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The levels of Akt , Bad , and eNOS ( total and phosphorylated ) proteins were measured . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
G 17 stimulated BAD phosphorylation , measured by both Western blots with anti phospho BAD antibodies and by in vitro Akt kinase assays using recombinant BAD as substrate . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
It has been demonstrated that PI 3 K / Akt and downstream phosphorylated Bad and proline rich Akt substrate survival signaling cascades are upregulated in surviving neurons in the ischemic brain that overexpresses copper zinc superoxide dismutase activity . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In conclusion , 1 , 25 ( OH ) 2D3 clearly protects keratinocytes against apoptosis ( 1 ) by activating the MEK / ERK and the PI 3K / Akt survival pathways and ( 2 ) by increasing the Bcl 2 to Bax and Bad ratio . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Finally , we showed that the inhibitory effect of LHRH on IGF 1 induced PKCalpha mediated Akt activation was associated with a marked reduction in Bad phosphorylation and a substantial decrease in the ability of IGF 1 to rescue alphaT 3 cells from apoptosis induced by serum starvation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Interestingly , survival signals such as phosphorylation of Akt and Bad seemed to be induced by the B [ a ] P / CPP compounds . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Cannabinoids down regulate PI3K / Akt and Erk signalling pathways and activate proapoptotic function of Bad protein . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
HGF protects corneal epithelial cells from apoptosis by the PI 3K / Akt 1 / Bad but not the ERK1 / 2 mediated signaling pathway . ^^^ HGF stimulated the downstream targets of Akt , glycogen synthase kinase ( GSK 3 ) , and Bad , a proapoptotic member of the Bcl 2 family , an effect that was blocked by PI 3K inhibitors but not by ERK1 / 2 inhibition . ^^^ Suppressing the expression of Akt by Akt siRNA led to a decrease in the phosphorylation of Bad and GSK 3 . ^^^ Moreover , in epithelial cells overexpressing active Akt 1 , Bad translocation was also prevented . ^^^ The results demonstrated that HGF , in a paracrine fashion , protects cells from apoptosis through a PI 3K / Akt / Bad pathway but not through an ERK1 / 2 pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We discuss several of the proposed mechanisms for the antiapoptotic effect of activated Akt , including the inhibition of the proapoptotic protein Bad , downregulation of death receptors , and enhancement of the glycolytic rate . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Combined treatment also resulted in pronounced reductions in levels of phospho Akt , glycogen synthase kinase 3 ( GSK 3 ) , p 70 ( S6K ) , mammalian target of rapamycin ( mTOR ) , forkhead transcription factor ( FKHR ) , caspase 9 , and Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Similarly , Akt mediate cell survival via the regulation of cell survival proteins and by blocking the function of proapoptotic Bad by phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
A series of distinct pathways are regulated by Akt that include the Forkhead family of transcription factors , GSK 3 beta , beta catenin , c Jun , CREB , Bad , IKK , and p 53 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Inactivation of Akt was associated with dephosphorylation of BAD , increased cytochrome c release , and activation of caspase 3 and caspase 9 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
These data demonstrate that both MEK 1 ERK1 / 2 and PI3K Akt signaling pathways are involved in CXCR 2 mediated neuroprotection and that multiple downstream signaling events , including RSKs , Bad , and CREB , are activated in this process . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Kallikrein / kinin protects against myocardial apoptosis after ischemia / reperfusion via Akt glycogen synthase kinase 3 and Akt Bad . 14 3 3 signaling pathways . ^^^ Delivery of the kallikrein gene increased phosphorylation of Src , Akt , glycogen synthase kinase ( GSK ) 3beta , and Bad ( Ser 136 ) but reduced caspase 3 activation in rat myocardium after reperfusion . ^^^ Moreover , kallikrein promoted Bad . 14 3 3 complex formation and inhibited Akt GSK 3beta dependent activation of caspase 3 , whereas caspase 3 administration caused reduction of the Bad . 14 3 3 complex , indicating an interaction between Akt GSK caspase 3 and Akt Bad . 14 3 3 signaling pathways . ^^^ In conclusion , kallikrein / kinin protects against cardiomyocyte apoptosis in vivo and in vitro via Akt Bad . 14 3 3 and Akt GSK 3beta caspase 3 signaling pathways . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Fungal extracts with binding activities were characterized for biochemical inhibition of Akt 1 to phosphorylate the downstream substrate protein Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Whereas IGF 1 mediated PI 3K / Akt signaling plays a pivotal role in cell survival by inactivating proapoptotic Bad protein and suppressing caspase activation , its stimulation of the PI 3K / p70 S6K cascade promotes proliferation . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Restoration of SHIP expression in Jurkat cells with an inducible expression system caused a 69 % reduction of phosphatidylinositol 3 , 4 , 5 trisphosphate ( PtdIns ( 3 , 4 , 5 ) P ( 3 ) ) and a 65 % reduction of Akt kinase activity , which was associated with reduced phosphorylation of glycogen synthase kinase 3beta ( GSK 3beta ) ( Ser 9 ) without changing the phosphorylation of Bad ( Ser 136 ) , FKHR ( Ser 256 ) or MAPK ( Thr 202 / Tyr 204 ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We also demonstrated a role for Akt activation in daunomycin induced protection through phosphorylation and inactivation of the Bcl 2 family proapoptotic factor Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Thus , vaticanol C induced inhibition of both ERK and Akt phosphorylation , resulting in reduced phosphorylation of Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , L 1 triggered neuroprotection was found to be associated with increased phosphorylation of extracellular signal regulated kinases 1 / 2 , Akt and Bad , and inhibition of caspases . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Inhibition of neutrophil apoptosis by TLR agonists in whole blood : involvement of the phosphoinositide 3 kinase / Akt and NF kappaB signaling pathways , leading to increased levels of Mcl 1 , A 1 , and phosphorylated Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The activation profiles of Akt regulated downstream effectors such as Bad , IKK , and nuclear factor kappaB ( NFkappaB ) were also examined . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The treatment of NS 398 induced upregulation of Bad and PTEN , and downregulation of phosphorylated Akt ( Thr 308 ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Finally , N cadherin mediated cell cell contacts initiated anti apoptotic signaling by increasing Akt and Bad phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
IGF 1 stimulation resulted in the formation of the insulin receptor substrate ( IRS ) 1 p 85 complex , an increase in PI 3 kinase activity , and activation of protein kinase B ( AKT / PKB ) and the bcl 2 family member bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
There was upregulation of PKB and Bad phosphorylation and NF kappaB activation in N formyl l methionyl l leucyl l phenylalanine stimulated neutrophils from thermally injured rats compared with the sham injured group . ^^^ These data suggest that the delay in neutrophil apoptosis with thermal injury is partly caused by activation of PI 3 kinase / PKB signaling and NF kappaB , which appeared to be related to the increased Bcl xl expression and phosphorylation of Bad , but not IAP expression . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Moreover , Akt phosphorylates and thus inactivates the pro apoptotic proteins BAD and Forkhead transcription factors ( FKHR , FKHRL 1 ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Reactive oxygen species are known to be significantly generated in the mitochondrial electron transport chain in the dysfunctional mitochondria during reperfusion after ischemia , and are also implicated in the survival signaling pathway that involves phosphatidylinositol 3 kinase ( PI 3 K ) , Akt , and downstream signaling molecules , like Bad , 14 3 3 , and the proline rich Akt substrate ( PRAS ) , and their bindings . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt / protein kinase B is a major cell survival pathway through phosphorylation of proapoptotic proteins Bad and Bax and of additional apoptotic pathways linked to Forkhead proteins glycogen synthase kinase 3beta and ASK 1 . ^^^ Nonphosphorylatable ArgBP2gamma 4A and SH 3 domain truncated mutant ArgBP2gamma inhibit Akt induced PAK 1 activation and reduce Akt and PAK 1 phosphorylation of Bad and antiapoptotic function . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Associated with the osmotically preconditioned state was the activation of Akt and the inactivation of the pro apoptotic factor , Bad , both events blocked by the inhibition of PI 3 kinase . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , binding to these receptors triggers the phosphatidyloinositol 3 kinase ( PI3K ) / Akt signal transduction system and , in consequence , leads to the inhibition of PCD by activating antiapoptotic proteins through the transcription factor NFkappaB and inhibiting proapoptotic signaling by Bad , caspase 9 , caspase 3 , and other effectors . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phosphorylation of Bad is not essential for PKB mediated survival signaling in hemopoietic cells . ^^^ One key question is whether the survival signaling effect of the PI 3 kinase pathway is mediated by PKB phosphorylation of Bad . ^^^ We confirm previous reports that if Bad is overexpressed or if active PKB is overexpressed , then the increased phosphorylation of Bad at Ser 136 is apparent . ^^^ In summary , our results suggest that Bad phosphorylation at any of the three major sites is not a required event for cytokine dependent cell survival , and in particular , the activation of PI 3 kinase / PKB pathway can be dissociated from phosphorylation of Bad at Ser136 . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Furthermore , there was a significant decrease in the activation of Akt / protein kinase B in the junctional zone of the placenta , as assessed by the phosphorylation status of Akt and the pro apoptotic protein BAD , a downstream target of the Akt signaling pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Risedronate facilitated dephosphorylation of Bad at Ser 112 , an ERK phosphorylation site , but not at Ser 136 , an Akt phosphorylation site . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Current work has identified exciting pathways , such as the Wnt pathway and the serine threonine kinase Akt , as central modulators that oversee cellular apoptosis and their downstream substrates that include Forkhead transcription factors , glycogen synthase kinase 3beta , mitochondrial dysfunction , Bad , and Bcl 10 ( L ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
However , survival signal transduction from Akt to Bad was blocked by calcium release after activation of PI 3 kinase / Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt / Bad signaling and motor neuron survival after spinal cord injury . ^^^ The serine threonine kinase Akt is a cell survival signaling pathway that inactivates the proapoptotic BCL 2 family protein Bad and promotes cell survival in cerebral ischemia . ^^^ Involvement of the Akt / Bad signaling pathway after spinal cord injury ( SCI ) is , however , uncertain . ^^^ Our results showed that phospho Akt ( serine 473 ) and phospho Bad ( serine 136 ) were significantly upregulated at 1 day after SCI . ^^^ In addition , phospho Akt and phospho Bad were colocalized in motor neurons that survived SCI and inhibition of PI 3 K reduced expression of phospho Akt and phospho Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In both cases , Akt kinase activation and Bad phosphorylation were observed . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Here , we show that phosphorylation of 14 3 3 by JNK releases the proapoptotic proteins Bad and FOXO3a from 14 3 3 and antagonizes the effects of Akt signaling . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We also observed the enhanced phosphorylation of Akt and its downstream substrates glycogen synthase kinase 3beta and proapoptotic Bad in the HCV replicon expressing cells . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Endogenous Akt , PAK 1 , and BAD are phosphorylated in attached MCF10A cells , but these phosphorylation events are all lost during the first 8 hours of detachment . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The use of specific antibodies shows that some proportion of PKB / Akt that is phosphorylated both on threonine 308 and serine 473 is maintained in the presence of E 7 in a PI 3 kinase independent manner , and is activated for phosphorylation of BAD , a known downstream target of PKB / Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Recent work has identified novel pathways , such as the Wnt pathway and the serine threonine kinase Akt , as central modulators that oversee cellular apoptosis and the formation of neurofibrillary tangles through their downstream substrates that include glycogen synthase kinase 3beta , Bad , and Bcl xL . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The results demonstrate a correlation between Akt activity , phosphorylation of Bad and resistance to interferon induced apoptosis in these cells . ^^^ The association of activation of Akt , phosphorylation of Bad and resistance to IFN beta induced apoptosis was further supported by the observation that disruption of the pathway in a more resistant cell line led to sensitization , and expression of an activated Akt in a more sensitive cell line led to increased resistance . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Abeta 25 35 alters Akt activity , resulting in Bad translocation and mitochondrial dysfunction in cerebrovascular endothelial cells . ^^^ Akt prevents the activation of death signaling events by facilitating the inactivation of proapoptotic proteins such as Bad . ^^^ We applied three strategies to show that Abeta 25 35 inactivation of Akt is causally related to Abeta 25 35 induced CEC death by preventing Bad activation and subsequent mitochondrial dysfunction ( reflected by the release of endonuclease G and Smac , two proapoptotic intermembranous proteins of the mitochondria ) . ^^^ Enhancement of Akt activity by a Tat Akt fusion protein , or by viral gene transfer of a constitutively active mutant of akt , reduced Bad activation , mitochondrial dysfunction , and CEC death . ^^^ Together , these results establish that the Akt Bad cascade is altered by Abeta 25 35 , resulting in CEC apoptosis . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Proteins for Western blot analysis included Fas associated death domain ( FADD ) and caspase 8 for extrinsic pathway , as well as Bcl 2 , Bcl XL , Bax , Bad , Bid , Akt , p Akt , and caspase 9 for intrinsic pathway . ^^^ Levels of FADD , Bax , Bad , and Bid were substantially increased in the TA induced myopathy group , whereas Bcl 2 , Bcl XL , Akt , p Akt , and caspase 9 did not change between control and myopathy groups . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Survival was not attributable to activation of NF kappaB or up regulation of anti apoptotic proteins , but coincided instead with activation of Akt and phosphorylation of the pro apoptotic protein Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Diallyl trisulfide , a constituent of processed garlic , inactivates Akt to trigger mitochondrial translocation of BAD and caspase mediated apoptosis in human prostate cancer cells . ^^^ DATS treatment ( 40 microM ) also caused a decrease in Ser ( 155 ) and Ser ( 136 ) phosphorylation of BAD ( a proapoptotic protein ) , which is a downstream target of Akt . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Estradiol prevents the injury induced decrease of Akt activation and Bad phosphorylation . ^^^ This study investigated whether estradiol modulates the anti apoptotic signal through the activation of Akt and its downstream targets , including Bad , Bcl 10 ( L ) , and 14 3 3 . ^^^ Potential activation was measured by phosphorylation of Akt at Ser 473 and Bad at Ser 136 using Western blot analysis . ^^^ Our findings suggest that estradiol prevents cell death due to brain injury and that Akt activation and Bad phosphorylation by estradiol mediated these protective effects . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
This was accompanied by quantitatively similar disparities in phosphoinositide 3 kinase activation and in Akt stimulated suppressors of apoptosis : glycogen synthase kinase 3beta , forkhead , and Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Similarly , the proapoptotic proteins Bax and BAD were significantly elevated , while the antiapoptotic proteins Bcl 2 , Bcl ( XL ) and Akt 1 were downregulated . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
However , the defect was associated with the disturbance of a tripartite complex formed by Akt , B Raf and Bag 1 , in addition to the absence of Bad phosphorylation at Ser 136 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
These increases are accompanied by a concomitant rise in phosphorylation of proapoptotic protein Bad , which is sequestered by the 14 3 3 family of proteins following phosphorylation by Akt , resulting in a reduction in nonphosphorylated Bad . ^^^ Increased levels of Bcl xL and phosphorylated forms of Akt and Bad and reduction in caspase 3 activity were observed throughout the entire length of the colonic crypt of aged rats . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In addition , FAK , Akt , PDK 1 , GSK 3beta , BAD , and PTEN were phosphorylated by ICAM 3 overexpression , resulting in enhanced cell proliferation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Activation of several cell death programs represented by Fas ligand , FADD ( Fas Associated Death Domain / Mort1 ) and the caspase cascade ( caspase 8 and 3 ) and survival programs represented by phosphorylated protein kinase B ( PKB / Akt ) , Bcl 2 associated death domain ( BAD ) , and cAMP responsive element binding protein ( CREB ) were examined using immunohistochemistry and Western blotting . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Other Akt downstream targets , BAD , caspase 9 and the Forkhead transcription factor ( FKHR ) , were not phosphorylated during the course of infection by EV 71 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
UDCA treatment prior to LCA occlusion , activated phosphorylation of Akt and Bad . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Treatment with mitomycin C resulted in the increased expression of Fas , FasL , Bad , and phosphorylated p 53 and a decreased level of phosphorylated AKT . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The sirtuin inhibitor nicotinamide enhances neuronal cell survival during acute anoxic injury through AKT , BAD , PARP , and mitochondrial associated `` anti apoptotic ' ' pathways . ^^^ We examined neuronal injury by trypan blue exclusion , DNA fragmentation , phosphatidylserine ( PS ) exposure , Akt 1 phosphorylation , Bad phosphorylation , mitochondrial membrane potential , caspase activity , cleavage of poly ( ADP ribose ) polymerase ( PARP ) , and mitogen activated protein kinases ( MAPKs ) phosphorylation . ^^^ We further demonstrate that NIC functions through Akt 1 activation , Bad phosphorylation , and the downstream modulation of mitochrondrial membrane potential , cytochrome c release , caspase 1 , 3 , and 8 like activities , and PARP integrity to prevent genomic DNA degradation and PS externalization during anoxia . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We found that both PI 3 K inhibitors , wortmannin and LY 294002 , markedly suppressed phosphorylation of Akt and Bad in HL 60 cells . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In humans , increased phosphorylation of the PKB signaling pathway related proteins Bad and forkhead transcription factor ( FKHR ) was detected in patients with TBI versus controls . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Because Akt / PKB has been shown to utilize distinct downstream effectors ( glycogen synthase kinase 3beta , FKHR / FOXO1a , BAD , and AFX / FOXO4 ) to regulate cell survival , we next determined which downstream effector ( s ) is activated by Akt / PKB to promote osteoclast survival . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In HSCs treated with TJ 135 , increased expression of p 53 and decreased expression of Bcl 2 and phosphorylated Akt and Bad were determined . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
However , inhibition of PI 3 kinase Akt pathway and subsequent translocation of Bad to mitochondria did not induce apoptosis itself . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
H11K directly binds to both Akt and AMPK and promotes their nuclear translocation and their association in a multiprotein complex , which results in a stimulation of survival mechanisms in cytosol and nucleus , including inhibition of proapoptotic effectors ( glycogen synthase kinase 3beta , Bad , and Foxo ) , activation of antiapoptotic effectors ( protein kinase Cepsilon , endothelial and inducible NO synthase isoforms , and heat shock protein 70 ) , increased expression of the hypoxia inducible factor 1alpha , and genomic switch to glucose utilization . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Contribution of the phosphatidylinositol 3 kinase / serine / threonine kinase ( PI3k / Akt ) pathway was assessed by measuring total and phosphorylated PI3K , Akt , BAD , and endothelial nitric oxide synthase ( eNOS ) proteins . ^^^ There was no significant induction of Akt activity or changes in phosphorylated BAD , Akt , or eNOS levels . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
We confirmed that wortmannin blocked Akt phosphorylation and the downstream targets of the PI3K / Akt cascade , such as BAD ( Bcl 2 associated death protein ) and nuclear factor kappaB in vivo by immunohistochemical staining and Western blotting . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
After the GnTV AS transfected cells were treated with ATRA , phosphorylated PKB and Bad were further decreased , while Bax and activated caspase 3 fragment were further increased , leading to the enhanced apoptosis in flow cytometry analysis when compared with GnTV AS cells not treated with ATRA . ^^^ It was speculated that the decreased phospho Bad resulted from the reduced phospho PKB and the up regulation of p 53 caused the elevated activity of Bax . ^^^ In the study of the molecular mechanism of this phenomenon , it was found that GnTV AS reduced the expressions of anti apoptotic proteins , such as phosphorylated protein kinase B and phosphorylated Bad as well as Bcl 2 and Bcl 10 ( L ) , and elevated those of pro apoptotic proteins , including Bax , full length caspase 3 and its activated fragments as well as anti oncoprotein p 53 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
This phenomenon was associated with a profound decrease in MAPK activation , reduction of BAD ( ser 112 ) phosphorylation and a paradoxical increase in the levels of AKT activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
AKT , glycogen synthase kinase ( GSK ) 3beta , BAD , and cleaved caspase 3 were activated by CS in ECs . ^^^ ECs transfected with kinase dead AKT ( KA 179 ) as well as plasmids containing a point mutation in the pleckstrin homology domain of AKT ( RC 25 ) not only prevented AKT , GSK 3beta , and BAD phosphorylation but also inhibited the CS induced increase in cell number as well as the CS induced protection against apoptosis ( both P < 0 . 05 ) . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
DHCR 24 knockout embryonic fibroblasts are susceptible to serum withdrawal induced apoptosis because of dysfunction of caveolae and insulin Akt Bad signaling . ^^^ However , the inhibition of external cholesterol supply by serum withdrawal induced apoptosis of DHCR 24 ( / ) MEFs , which was associated with a marked decrease in the intracellular and plasma membrane cholesterol levels , Akt inactivation , and Bad dephosphorylation . ^^^ Insulin is an antiapoptotic factor capable of stimulating the Akt Bad cascade , and its receptor ( IR ) is enriched in caveolae , cholesterol rich microdomains of plasma membrane . ^^^ Consistent with these findings , insulin dependent phosphorylations of insulin receptor substrate 1 , Akt , and Bad were impaired in the cholesterol depleted MEFs . ^^^ These results demonstrate that the cellular cholesterol biosynthesis is critical for the activation and maintenance of the Akt Bad cell survival cascade in response to growth factors such as insulin . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Phosphorylation of Akt and Bad was visualized by Western blotting and phospho Akt localization by confocal microscopy . ^^^ The antiapoptotic signaling of ANP was most likely caused by phosphorylation of Akt and Bad , since pretreatment with PI 3 kinase inhibitor WM abrogated the ANP induced reduction of caspase 3 activity . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
BACKGROUND : This study evaluated the role of protein kinase B ( PKB ) , phosphatidylinositol 3 kinase ( PI 3 K ) , Bcl 2 associated death protein ( BAD ) and mitogen activated protein kinases ( MAPKs ) in normothermic ischaemia reperfusion ( IR ) induced apoptosis in rat liver . ^^^ PI 3 K , PKB , BAD and MAPK activities were measured in ischaemic and non ischaemic lobes at various times after reperfusion . ^^^ In controls , PI 3 K and PKB activities and BAD phosphorylation were inhibited in ischaemic liver lobes . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt phosphorylation , Bax , Bcl 2 , Bad , and phosphorylated Bad ( phospho Bad ) expression was assessed by immunoblotting . ^^^ Akt phosphorylation and Bcl 2 and phospho Bad expression increased after isoflurane preconditioning , whereas Bax expression decreased . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
METHODS : Quantitative Western blot analysis and immunocytochemistry were used to examine the activation status and localization of key components of the Akt signaling cascade ( Akt , BAD , Forkhead [ FKHR ] , HSP 27 , mitogen activated protein ( MAP ) kinase kinase 3 and 6 ( MKK3 / 6 ) , the tumor suppressor phosphatase PTEN , and the cytoplasmic protein tyrosine kinase cSrc p 60 ) , in the retina of the rd mouse in comparison with the control . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
OV activated the Akt , extracellular signal regulated protein kinase , and p 38 mitogen activated protein kinase pathways , which are involved in Bad phosphorylation . ^^^ SEC apoptosis in vivo was associated with dephosphorylation of Bad , Akt , and extracellular signal regulated protein kinase , which was blocked by OV treatment . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Activation of PI3K by K 1 results in activation of Akt kinase and mammalian target of rapamycin and inactivation of the proapoptotic proteins FKHR , glycogen synthase kinase 3 , and Bad , which are events indicative of cell survival . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Western blot analysis indicated that KP 372 1 decreased the phosphorylation of AKT on both Ser ( 473 ) and Thr ( 308 ) ; abrogated the phosphorylation of p70S6 kinase , BAD , and Foxo3a via PI3K / AKT signaling ; and down regulated expression of PIM 1 through direct inhibition of FLT 3 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The capacity of nicotinamide to govern not only intrinsic cellular integrity , but also extrinsic cellular inflammation rests with the modulation of a host of cellular targets that involve mitochondrial membrane potential , poly ( ADP ribose ) polymerase , protein kinase B ( Akt ) , Forkhead transcription factors , Bad , caspases , and microglial activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
The presence of p Akt was accompanied by the phosphorylation of p 27 ( kip 1 ) , FRKHL 1 , MDM 2 , Bad , mTOR , and p70S6K . ^^^ Inhibition of the PI3K / Akt pathway in the MCL cell lines abrogated or reduced the phosphorylation of Akt , p 27 ( kip 1 ) , FRKHL 1 , MDM 2 , Bad , mTOR , GSK 3beta , IkappaB , and led to cell cycle arrest and apoptosis . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
However , no significant association of Bad translocation with phosphatidylinositol 3 kinase / Akt and protein kinase A signaling pathways was noted when treated cells were examined in relation to phosphorylation status of Bad by Western blot and localization of Bad to mitochondria by confocal analysis . ^^^ Furthermore , THC treatment decreased the Bad phosphorylation at Ser ( 112 ) but failed to alter the level of phospho Bad on site Ser ( 136 ) that has been reported to be associated with phosphatidylinositol 3 kinase / Akt signal pathway . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
In C . trachomatis infected cells , the PI3K pathway activates AKT / protein kinase B , which leads to maintenance of the pro apoptotic protein BAD in a phosphorylated state . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Overexpression of dominant negative Akt attenuated agonist induced phosphorylation of BAD , but not that of ERK1 / 2 and CREB . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Expression of phospho Akt and phospho BAD was measured by Western blotting . ^^^ At 1 10 ng 10 ml 1 concentrations , TNF a induced phosphorylation of Akt and BAD in a time and concentration dependent manner . ^^^ Activation of Akt and BAD may have an important role in this process . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
This applies to tumor suppressors of the Bax / Bcl 2 family ( Bax , Bad , Bid , Bim ) , several protein kinases ( Akt , ASK 1 , hexokinase ) , p 53 , NF kappaB , and nuclear orphan receptors such as TR3 / Nur77 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Ang 2 phosphorylated both Akt , Bcl 2 and Bad , which was blocked by NAC , PD 098059 or SP 600125 , but not by SB 202190 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
This occurred concomitantly with altered phosphorylation of the pro apoptotic proteins GSK3beta and Bad , despite normal levels of Akt activation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Notably , we also found that doxorubicin provoked apoptosis included p 38 MAPK mediated inhibition of Akt and Bad phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Isoflurane decreased phosphorylation of the pro apoptotic cofactors Bad and p90RSK and increased Akt phosphorylation . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Upstream proteins known to phosphorylate BAD include Akt , extracellular signal regulated kinase / mitogen activated protein kinase ( Erk / MAPK ) , Pim 1 and Pim 2 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Akt activation associated with hepatic IPC suppressed the activity of several modulators of apoptosis , including Bad , glycogen synthase kinase beta , and caspase 3 . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
These apoptotic markers were suppressed by the addition of ( 20S ) Rg 3 . ( 20S ) Rg 3 increased the expression of Bax and conversely decreased Bcl 2 . ( 20S ) Rg 3 potently induced a rapid and sustained Akt activation and Bad phosphorylation , resulting in the inhibition of mitochondrial cytochrome c release . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
Additionally , ethanol exposed cells display a blunting of TNF alpha induced Akt activation and Bcl 2 antagonist of cell death phosphorylation that may account , in part , for the increased sensitivity of the mitochondria to Bax mediated damage . . ^^^
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
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Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92934 and P31749	Pubmed	SVM Score :0.0
NA