| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that the activity of the serine / threonine kinase Akt / PKB is required in a signaling cascade that leads to phosphorylation and inactivation of 4E BP 1 . ^^^ PI 3 kinase elicits the phosphorylation of 4E BP 1 in a wortmannin and rapamycin sensitive manner , whereas activated Akt mediated phosphorylation of 4E BP 1 is wortmannin resistant but rapamycin sensitive . ^^^ A dominant negative mutant of Akt blocks insulin mediated phosphorylation of 4E BP 1 , indicating that Akt is required for the in vivo phosphorylation of 4E BP 1 . ^^^ Importantly , an activated Akt induces phosphorylation of 4E BP 1 on the same sites that are phosphorylated upon serum stimulation . ^^^ Similar to what has been observed with serum and growth factors , phosphorylation of 4E BP 1 by Akt inhibits the interaction between 4E BP 1 and eIF 4E . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| PTEN / MMAC1 impairs activation of endogenous Akt in cells and inhibits phosphorylation of 4E BP 1 , a downstream target of the PI 3 kinase / Akt pathway involved in protein translation , whereas a catalytically inactive , dominant negative PTEN / MMAC1 mutant enhances 4E BP 1 phosphorylation . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , all the activated PKB mutants , regardless of constitutive membrane localization , induce 4E BP 1 phosphorylation and the interfering PKB mutant blocks insulin induced 4E BP 1 phosphorylation . ^^^ The results demonstrate that PKB mediates S6K1 activation only as a function of constitutive membrane localization , whereas the activation of PKB appears both necessary and sufficient to induce 4E BP 1 phosphorylation independently of its intracellular location . . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Requirement for Akt ( protein kinase B ) in insulin induced activation of glycogen synthase and phosphorylation of 4E BP 1 ( PHAS 1 ) . ^^^ The roles of Akt ( protein kinase B ) and the atypical lambda isoform of protein kinase C ( PKClambda ) , both of which act downstream of phosphoinositide 3 kinase , in the activation of glycogen synthase and phosphorylation of 4E BP 1 ( PHAS 1 ) in response to insulin were investigated . ^^^ Insulin induced phosphorylation of 4E BP 1 was inhibited by Akt AA in Chinese hamster ovary cells . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Because phosphatidylinositol 3 kinase ( PI3K ) and protein kinase B ( PKB ) , in concert with mTOR , inactivate 4E BP 1 , we explored their role in GLUT 1 protein expression . ^^^ Increased GLUT 1 mRNA translation appears to occur via the PI3K / PKB / mTOR / 4E BP 1 cascade . . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| These results add substantially to the emerging evidence that nutrients themselves modulate functions of mammalian cells and indicate that ( 1 ) nutrients modulate the activation of eIF2B and eEF 2 through as yet unidentified mechanisms and ( 2 ) regulation of p 70 S6 kinase and 4E BP 1 by insulin requires other inputs in addition to protein kinase B . . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Although the PE induced phosphorylation of 4E BP 1 was blocked by the phosphatidylinositol 3 kinase inhibitor LY 294002 , neither phosphatidylinositol 3 kinase nor Akt , its downstream effector , is activated in cells treated with PE ( Ballou , L . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Insulin also activated protein kinase B ( PKB ) , which may lie upstream of p 70 S6k and 4E BP 1 , with the activation of the different isoforms being in the order alpha > beta > gamma . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Therefore TPA can induce eIF4F assembly , protein synthesis , and the phosphorylation of p 70 S6 kinase and 4E BP 1 independently of both phosphatidylinositol 3 kinase and protein kinase B . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| One mechanism involves PI3K / PKB dependent phosphorylation of 4E BP 1 , which dissociates from eIF4E , allowing initiation of translation from the 7 methylGTP cap of mRNAs . ^^^ Insulin or H ( 2 ) O ( 2 ) increased the phosphorylation ( activation ) of PKB through PI3K , but , whereas insulin induced a sustained response , the response to H ( 2 ) O ( 2 ) was transient . 4E BP 1 was phosphorylated in unstimulated cells , and 4E BP 1 phosphorylation was increased by insulin . ^^^ Regulation of protein kinase B and 4E BP 1 by oxidative stress in cardiac myocytes . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| The increase in PKB activity was reflected in the hyperphosphorylation of phosphorylated , heat and acid stable regulated by insulin protein ( PHAS 1 ; also termed 4E BP 1 , for eukaryotic initiation factor 4E binding protein 1 ) , a protein involved in the regulation of translation initiation . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| A mutant of Akt that retains kinase activity but does not induce phosphorylation of S6K or of 4E BP 1 fails to transform chicken embryo fibroblasts , suggesting a correlation between the oncogenicity of Akt and phosphorylation of S6K and 4E BP 1 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Studies suggested that insulin or growth factor induced 4E BP 1 phosphorylation is mediated by phosphatidylinositol 3 kinase ( PI 3 kinase ) and its downstream protein kinase , Akt . ^^^ The PI 3 kinase inhibitor , wortmannin , did not block UVB induced 4E BP 1 phosphorylation , but blocked both UVB and insulin induced activation of PI 3 kinase and phosphorylation of Akt . 4E BP 1 phosphorylation was blocked in JB 6 Cl 41 cells expressing a dominant negative p 38 kinase or dominant negative MSK 1 , but not in cells expressing dominant negative ERK 2 , JNK 1 , or PI 3 kinase p 85 subunit . ^^^ The p38 / MSK1 pathway , but not PI 3 kinase or Akt , is required for mediating the UVB induced 4E BP 1 phosphorylation . . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Because activation of the PI 3 K / AKT as well as RAS MEK ERK pathways may result in downstream stimulation of the p 70 ( S6K ) ( p 70 ) and phosphorylation of the 4E BP 1 translational repressor , we assessed these potential molecular targets in IL 6 treated myeloma cells . ^^^ In contrast , IL 6 induced phosphorylation of 4E BP 1 was inhibited by rapamycin , wortmannin , and dominant negative AKT but ERK inhibitors had no effect , indicating ERK function was dispensable . ^^^ In keeping with these data , a dominant active AKT mutant was sufficient to induce 4E BP 1 phosphorylation but could not by itself activate p 70 kinase activity . ^^^ These results indicate that both ERK and PI 3 K / AKT pathways are required for optimal IL 6 induced p 70 activity , but PI 3 K / AKT is sufficient for 4E BP 1 phosphorylation . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| The regulation of 4E BP 1 phosphorylation by alpha 6 beta 4 derives from the ability of this integrin to activate the PI 3K Akt pathway and , consequently , the rapamycin sensitive kinase mTOR that can phosphorylate 4E BP 1 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| On the other hand , LY 294002 and wortmannin , specific inhibitors of PI3K , prevented PDGF BB induced phosphorylation of Akt and its downstream effector molecules , p70S6K , ribosomal protein S 6 , 4E BP 1 , and eIF4E . ^^^ NEM also abrogated the phosphorylation of p70S6K , ribosomal protein S 6 , 4E BP 1 , and eIF4E induced by PDGF BB , suggesting that thiol alkylation interferes with the PI3K / Akt pathway at the level of Akt . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| In addition , 5 ( S ) HETE stimulated phosphatidylinositol 3 kinase ( PI 3 kinase ) activity and phosphorylation of its downstream targets Akt , p70S6K , and 4E BP 1 and their effector molecules ribosomal protein S 6 and eIF4E . ^^^ Interestingly , AG 490 attenuated 5 ( S ) HETE induced PI 3 kinase activity and phosphorylation of Akt , p70S6K , ribosomal protein S 6 , 4E BP 1 , and eIF4E . 5 ( S ) HETE induced the expression of basic fibroblast growth factor 2 ( bFGF 2 ) in a Jak 2 and PI 3 kinase dependent manner . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Rectus muscle protein synthesis was measured at the end of the clamp , and the phosphorylation states of protein kinase B ( Akt ) , eukaryotic initiation factor 4E binding protein 1 ( 4E BP 1 ) , and ribosomal protein S 6 kinase ( p 70 ( S6K ) ) were quantitated before and after the insulin clamp . ^^^ The basal phosphorylation states of Akt , 4E BP 1 , and p 70 ( S6K ) were similar between ADX and Sham rats . ^^^ Insulin significantly enhanced the phosphorylation of Akt ( P < 0 . 03 ) , 4E BP 1 ( P = 0 . 003 ) , and p 70 ( S6K ) ( P < 0 . 002 ) in ADX but not in Sham rats . ^^^ Glucocorticoid replacement blunted the effect of insulin on Akt , 4E BP 1 , and p 70 ( S6K ) phosphorylation and protein synthesis . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Hyperosmolarity induces sustained suppression of p70S6 kinase and 4E BP 1 hyperphosphorylation by insulin , whereas insulin induced tyrosine phosphorylation of the insulin receptor ( IR ) beta subunit and the IR substrates IRS 1 and IRS 2 , recruitment of the phosphoinositide 3 kinase ( PI 3 kinase ) regulatory subunit p 85 to the receptor substrates as well as PI 3 kinase activation , and Ser 473 phosphorylation of protein kinase B and Thr 410 / 403 phosphorylation of PKC zeta / lambda are largely unaffected under hyperosmotic conditions . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| At the molecular level , estrogen stimulates the rapid activation of specific signal transduction pathways , and of particular interest is the activation of Akt ( protein kinase B ) , a key signal transduction intermediate that initiates protein translation by alleviating the downstream translational repression of eukaryotic initiation factor 4E binding protein 1 ( 4E BP 1 ) . ^^^ Using a well established in vitro model system of differentiated NG 108 15 neurons to investigate such rapid signaling effects of estrogen , we show that estrogen stimulates the phosphorylation of Akt , an indication of kinase activation , as well as the phosphorylation of 4E BP 1 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , despite appropriate stimulation of IGF 1 receptor , IRS 1 and PKB , EtOH impairs IGF 1 signaling via S6K1 and 4E BP 1 pathways , and this defect is regulated in a glucocorticoid and TNF independent manner . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| This inhibition was not accounted for by changes in the upstream stimulatory pathway , because caerulein activated Akt as well as phosphorylating the downstream effectors of mTOR , 4E BP 1 , and ribosomal protein S 6 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| VEGF stimulated eukaryotic initiation factor 4E binding protein ( 4E BP 1 ) phosphorylation , which was dependent on activation of PI 3 kinase and Akt . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Basal PI3K activity in long term estrogen deprived MCF 7 cells ( LTED ) was elevated as evidenced by increased phosphorylation of three downstream effectors , Akt , p 70 S6 kinase , and eukaryotic initiation factor 4E binding protein ( 4E BP 1 ) , which was blocked by the specific inhibitor of PI3K , LY 294002 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Replacing Ser ( 302 ) with alanine significantly reduced insulin stimulated tyrosine phosphorylation of IRS 1 and p 85 binding and reduced insulin stimulated phosphorylation of p 70 ( S6K ) , ribosomal S 6 protein , and 4E BP 1 ; however , this mutation had no effect on insulin stimulated Akt or glycogen synthase kinase 3beta phosphorylation . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| ADX had no effect on myocardial Akt or GSK 3 [ glycogen synthase ( GS ) kinase 3 ] phosphorylation , but it decreased the phosphorylation of eukaryotic initiation factor 4E binding protein 1 ( 4E BP 1 ) and ribosomal protein S 6 kinase ( p 70 ( S6K ) ) ( P < 0 . 003 for both ) . ^^^ Insulin enhanced the phosphorylation of Akt ( P < 0 . 04 ) , 4E BP 1 ( P < 0 . 002 ) , and p 70 ( S6K ) ( P < 0 . 0001 ) in ADX , but not in sham rats . ^^^ Dexamethasone restored the levels of 4E BP 1 and p 70 ( S6K ) phosphorylation and abrogated the insulin stimulated Akt , 4E BP 1 , and p 70 ( S6K ) phosphorylation . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Toxin B , which inactivates Rho GTPases , markedly impaired PLD 1 activation and phosphorylation of Akt , p 70 ( S6K ) , and 4E BP 1 induced by LPA but had a minimal or no effect on the actions of PDGF . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Incorporation of L [ 1 13C ] leucine in muscle proteins ( fractional synthesis rate , FSR ) was measured in vastus lateralis , before and during a euglycemic hyperinsulinemic hyperaminoacidemic clamp , together with Western blot analysis of protein kinase B ( PKB ) , mTOR , 4E BP 1 , and S6K1 phosphorylation . ^^^ Phosphorylation of PKB , mTOR , and 4E BP 1 were similarly increased by insulin and amino acid in both groups , except for S6K1 phosphorylation , which was not stimulated in elderly subjects . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| We hypothesize that overexpressed eIF4E is functionally active in tumor margins through activation of the Akt / mammalian target of rapamycin ( mTOR ) pathway EXPERIMENTAL DESIGN : Western blots and / or immunohistochemistry were performed to determine whether phosphorylation of mTOR and activation of its downstream molecules eIF4E binding protein 1 ( 4E BP 1 ) and p 70 S6 kinase and the upstream modulator of mTOR , Akt , were expressed in margins overexpressing eIF4E . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Consistent with unimpaired insulin activation of PKB , the stimulation of Ser 21 and Ser 9 phosphorylation of glycogen synthase kinase 3 alpha and beta , and the apparent phosphorylation level of 4E BP 1 ( eukaryotic initiation factor 4 binding protein 1 ) , were similar in transduced and control hepatocytes . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Expression of a kinase dead PKC eta ( PKC etaKR ) construct reduced the basal and PMA evoked proliferation of PKC eta expressing U 251 GBM cells , as well as abrogated the PMA induced activation of Akt , mTOR , and the mTOR targets 4E BP 1 and STAT 3 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Mainly , the abundance and phosphorylation state ( activity ) of the cell cycle dependent kinases MAPK and Akt ( PKB ) and their potential targets eIF4E ( eukaryotic initiation factor 4E ) and 4E BP 1 ( 4E binding protein , eIF4E repressor protein ) were investigated . ^^^ The results show that alpha ZOL has apparently only a slight influence on the phosphorylation state of MAP kinases , Akt and on eIF4E and 4E BP 1 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of the Akt / mammalian target of rapamycin / 4E BP 1 pathway by ErbB 2 overexpression predicts tumor progression in breast cancers . ^^^ The Akt / mammalian target of rapamycin ( mTOR ) / 4E BP 1 pathway is considered to be a central regulator of protein synthesis , involving the regulation of cell proliferation , differentiation , and survival . ^^^ However , the activation status of the Akt / mTOR / 4E BP 1 pathway and its potential roles in breast cancers remain unknown . ^^^ Thus , we examined 165 invasive breast cancers with specific antibodies for the phosphorylation of Akt , mTOR , and 4E BP 1 by immunohistochemistry and compared them with normal breast epithelium , fibroadenoma , intraductal hyperplasia , and ductal carcinoma in situ . ^^^ We discovered that the phosphorylation of Akt , mTOR , and 4E BP 1 increased progressively from normal breast epithelium to hyperplasia and abnormal hyperplasia to tumor invasion . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Moreover , reduced insulinaemia was associated with 4E BP 1 dephosphorylation , enhanced assembly of the 4E BP 1 eIF4E inactive complex and hypophosphorylation of eIF4E , p70S6k and protein kinase B , key intermediates in the regulation of translation initiation and protein synthesis . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Investigation of proteins in the phosphatidylinositol 3 ' kinase / Akt signaling pathway in PTEN delivered mouse lung revealed that the PTEN protein was highly expressed , whereas the protein levels of PDK 1 , total Akt 1 , phospho ( Thr 308 ) Akt , phospho ( Ser 2448 ) mTOR , p70S6K , and 4E BP 1 were decreased to varying degrees . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Several downstream effectors of Akt signalling , including glycogen synthase kinase 3 ( GSK 3 ) alpha and beta and mTOR substrates 4E BP 1 and p 70 S6 kinase , were also phosphorylated in HL cells . ^^^ Importantly , HRS cells of primary tumour samples not only expressed high levels of activated Akt but also displayed phosphorylation of downstream targets of Akt activation including GSK 3 , 4E BP 1 , and p 70 S6 Kinase . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| There was also suppression in phosphorylated PKB Thr ( 308 ) , forkhead in rhabdomyosarcoma , S6K1 , S 6 , 4E BP 1 , and signal transducers and activators of transcription 3 Tyr ( 705 ) and Ser ( 727 ) protein levels with ILK inhibition by QLT 0254 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| In contrast , the ability of a maximally stimulating dose of insulin to increase the phosphorylation of PKB , 4E BP 1 , S6K1 , or mTOR was not altered 20 min after intravenous injection . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| These effects of IGF 1 were associated with increased phosphorylation of Akt , GSK 3beta , and the mTOR downstream targets p 70 ( S6K ) and 4E BP 1 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Protein kinase B , ribosomal protein S 6 kinase 1 ( S6K1 ) , and eukaryotic initiation factor ( eIF ) 4E binding protein 1 ( 4E BP 1 ) were more phosphorylated , and assembly of the inactive eukaryotic initiation factor 4E . 4E BP 1 complex in muscle and liver was reduced in the fed state ( P < 0 . 001 ) and were not consistently affected by dietary protein level . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| We now know that three of the most frequent mutations in cancer constitutively activate PI3Kalpha and , when expressed in cells , they drive the oncogenic transformation and chronic activation of downstream signalling by molecules such as PKB , S6K and 4E bp 1 that is commonly seen in cancer cells . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of the potential upstream regulators of 4E BP 1 and S6K1 phosphorylation via PKB and mTOR was also observed . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Akt , p 70 S6 kinase , and 4E BP 1 were induced and phosphorylated in rat glomerular lysates after 12 weeks of STZ injection when mesangial and glomerular hypertrophy was observed . ^^^ Furthermore , less phosphorylated Akt positive or 4E BP 1 positive areas were found in STZ treated Gas 6 knockout mice than in STZ treated wild type mice . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| The abundance and phosphorylation state ( activity ) of the cell cycle dependent kinases MAPk and Akt ( PKB ) and their potential targets eIF 4E ( eukaryotic initiation factor 4E ) and 4E BP 1 ( 4E binding protein , eIF4E repressor protein ) were examined . ^^^ The results show that DNV in vitro strongly reduce the abundance of p 38 MAPk , protein kinase Akt and the alpha and beta 4E BP 1 bands . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Lovastatin decreases protein kinase B ( PKB ) / Akt phosphorylation , and its downstream effectors , p70S6K and the eukaryotic initiation factor 4E ( eIF4E ) regulatory protein 1 , 4E BP 1 , in a concentration dependent manner , and reduces p70S6K expression . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| In an effort to generate novel anticancer agents , a series of hybrids of alpha methylene gamma lactones and 2 phenyl indoles has been synthesized and evaluated for inhibition activities on the phosphorylation of AKT , mTOR , p70S6 kinase , and 4E BP 1 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| PPAR gamma activation reduced ANG 2 induced growth associated with inhibition of ERK 1 / 2 , Akt , 4E BP 1 , and SHIP 2 . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NAC and DPI also blocked Akt activation which is required for 4E BP 1 phosphorylation . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Constitutively active mutants of Ras , PI3K , and Akt , or RNA interference ( RNAi ) knockdown of lipid phosphatase PTEN ( phosphatase and tensin homolog deleted on chromosome Ten ) , induced growth and elaboration of dendrites that was blocked by mTOR inhibitor rapamycin and / or by overexpression of eIF 4E binding protein 1 ( 4E BP 1 ) , which inhibits translation of 5 ' capped mRNAs . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| We found that fibronectin stimulated the phosphorylation of Akt , an upstream inducer of mTOR , and induced the phosphorylation of p70S6K1 and eukaryotic initiation factor 4E binding protein 1 ( 4E BP 1 ) , two downstream targets of mTOR in NSCLC cells ( H 1792 and H 1838 ) , whereas it inhibited the phosphatase and tensin homologue deleted on chromosome 10 , a tumor suppressor protein that antagonizes the phosphatidylinositol 3 kinase / Akt signal . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Current models suggest that AKT acts directly , or indirectly via the TSC complex , to activate the mammalian target of rapamycin ( mTOR ) as the main downstream mediator of AKT signaling . mTOR activation results in subsequent activation of S6K and STAT 3 , as well as suppression ( i . e . , phosphorylation ) of 4E BP 1 , leading to cell cycle progression and inhibition of apoptosis . ^^^ Within individual tumors , increased expression levels of p TSC 2 , p mTOR , p 4E BP 1 , p S6K , p S 6 , and p STAT 3 were found in regions defined by elevated AKT activation . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| The EGF induced activation of PI 3 kinase Akt mTOR regulates phosphorylation of molecules including ribosomal protein S 6 , eIF4E and 4E BP 1 that influence translational control in HC 11 cells undergoing lactogenic differentiation . . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of Akt stimulated signaling through the protein kinase mammalian target of rapamycin , as evidenced by increased phosphorylation of two of its effectors , the ribosomal protein S 6 kinase and the eukaryotic initiation factor eIF4E binding protein 1 . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| This conditionally active Akt was also found to rapidly stimulate in these cells the phosphorylation of properties of PHAS 1 , a key protein in the regulation of protein synthesis . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activating PKB with 4 hydroxytamoxifen mimicked insulin by decreasing mTOR reactivity with mTAb 1 and by increasing the PHAS 1 kinase activity of mTOR . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| SI / R also induced the phosphorylation of PHAS 1 , a downstream Akt target , in a wortmannin dependent manner . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Transient transfection assays with mTOR mutants bearing Ala substitutions at Ser 2448 and / or Thr 2446 indicated that AKT dependent mTOR phosphorylation was not essential for either PHAS 1 phosphorylation or p70S6K activation in HEK cells . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| To understand the role of arachidonic acid ( AA ) in regulating vascular smooth muscle cell ( VSMC ) growth , its effects on phosphorylation of Akt , S6K1 , ribosomal protein S 6 , 4EBP1 , and eIF4E were studied . ^^^ Arachidonic acid stimulated phosphorylation of Akt , S6K1 , ribosomal protein S 6 , 4EBP1 , and eIF4E in a time dependent manner in VSMC . ^^^ Metabolic conversion of AA via the LOX / MOX and / or COX pathways , to some extent , was required for its effects on the phosphorylation of Akt , S6K1 , ribosomal protein S 6 , 4EBP1 , and eIF4E . ^^^ LY 294002 , an inhibitor of PI3K , completely blocked AA induced phosphorylation of Akt , S6K1 , ribosomal protein S 6 , 4EBP1 , and eIF4E , suggesting a role for PI3K in these effects . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| Whereas kinase inactive mTOR did not enhance the decreases in cell size and glycolysis induced by rapamycin , expression of this mTOR mutant significantly enhanced the inhibitory effects of rapamycin on cell proliferation , 4EBP1 phosphorylation , and Akt activity . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| In contrast , overexpression of p 70 ribosomal S 6 kinase ( p70S6K ) and protein kinase B ( Akt ) enhanced phosphorylation of 4EBP1 and eIF4E binding affinity to the cap region of mRNA . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| STUDY DESIGN : Western blotting and immunohistochemistry were used to investigate the expression of estrogen receptor , progesterone receptor , HER 2 , AKT , and 4EBP1 proteins in 27 atrophic endometria , 31 grade 1 and 24 grade 3 endometrioid endometrial cancers , and 19 malignant mixed mllerian tumors . ^^^ RESULTS : Malignant mixed mllerian tumors and grade 3 endometrioid endometrial cancers demonstrated higher levels of AKT and 4EBP1 activation and hormone receptor loss compared with grade 1 endometrioid endometrial cancers and atrophic samples . ^^^ |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| We conclude from these studies that meal feeding stimulates at least 2 signal pathways in cardiac muscle that raises phosphorylation of eIF4G and 4EBP1 during meal feeding and results in sustained increases in phosphorylation of PKB and PKC epsilon . . ^^^ |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13541 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|