| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| Insulin induced phosphorylation and activation of endogenous or recombinant PDE3B in 3T3 L 1 adipocytes have now been shown to be inhibited by a dominant negative mutant of the serine threonine kinase Akt , suggesting that Akt is necessary for insulin induced phosphorylation and activation of PDE3B . ^^^ Serine 273 of mouse PDE3B is located within a motif ( RXRXXS ) that is preferentially phosphorylated by Akt . ^^^ A mutant PDE3B in which serine 273 was replaced by alanine was not phosphorylated either in response to insulin in intact cells or by purified Akt in vitro . ^^^ In contrast , PDE3B mutants in which alanine was substituted for either serine 296 or serine 421 , each of which lies within a sequence ( RRXS ) preferentially phosphorylated by cAMP dependent protein kinase , were phosphorylated by Akt in vitro or in response to insulin in intact cells . ^^^ These results suggest that PDE3B is a physiological substrate of Akt and that Akt mediated phosphorylation of PDE3B on serine 273 is important for insulin induced activation of PDE3B . . ^^^ |
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| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| Both PI 3 kinase and PKB are important for glucose transport and intracellular protein translocation while PKB does not appear to play an important role for the antilipolytic effect or activation of PDE3B in response to insulin . . ^^^ |
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| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| In the absence of insulin like growth factor 1 ( IGF 1 ) , activities of PKB , cyclic nucleotide phosphodiesterase 3B ( PDE3B ) , and PDE 4 were similar in nontransfected FDCP 2 cells , mock transfected ( F / V ) cells , and F / B and F / B cells . ^^^ In F / V cells , IGF 1 increased PKB , PDE3B , and PDE 4 activities approximately 2 fold . ^^^ In F / B cells , IGF 1 , in a wortmannin sensitive manner , increased PKB activity approximately 10 fold and PDE3B phosphorylation and activity ( approximately 4 fold ) , but increased PDE 4 to the same extent as in F / V cells . ^^^ In F / B * cells , in the absence of IGF 1 , PKB activity was markedly increased ( approximately 10 fold ) and PDE3B was phosphorylated and activated ( 3 to 4 fold ) ; wortmannin inhibited these effects . ^^^ In F / B * cells , IGF 1 had little further effect on PKB and activation / phosphorylation of PDE3B . ^^^ |
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| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| In adipocytes , protein kinase B ( PKB ) has been suggested to be the enzyme that phosphorylates phosphodiesterase 3B ( PDE3B ) , a key enzyme in insulin ' s antilipolytic signalling pathway . ^^^ |
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| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| Insulin induced phosphorylation and activation of PDE3B is phosphatidylinositol 3 kinase ( PI 3 K ) and Akt dependent , but the precise mechanism of PDE3B activation is not fully understood . ^^^ |
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| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q13370 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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