| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.63989054 |
| We found that Akt phosphorylated and modestly activated Pak 1 in vitro . 0.63989054^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.57933369 |
| CYP 2C9 overexpression in endothelial cells increased the association of PAK 1 with Rac , a response also elicited by the CYP 2C9 product 11 , 12 EET . 0.57933369^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| ArgBP2gamma interacts with Akt and p 21 activated kinase 1 and promotes cell survival . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The first group , which includes PAK 1 , is regulated by Rac and Cdc42Hs , and activators have been identified . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Unlike PAK 1 , MIHCK is activated by Rac only in the presence of phospholipid . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| In contrast , spinophilin binding suppresses the ability of Tiam to activate Pak 1 , a different Rac effector . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Cdc 42 and Rac and their downstream target PAK 1 were activated following adhesion to laminin 5 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| PAK 1 and PAK 3 serve as downstream effectors of Rac in regulating spine and synapse formation . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Potential upstream regulators of myosin 2 are described , including DgkA , cGMP , cAMP and PAKa , a target for Rac GTPases . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Rho family GTPases regulate cytoskeletal dynamics in various cell types . p 21 activated kinase 1 ( PAK 1 ) is one of the downstream effectors of Rac and Cdc 42 which has been implicated as a mediator of polarized cytoskeletal changes in fibroblasts . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| We describe an assay that measures the formation of Rac GTP and Cdc 42 GTP based on their specific binding to the p 21 binding domain of p 21 activated kinase 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| In this study , we show that p 21 activated kinase 1 , the activity of which is regulated by the GTP bound form of Cdc 42 and Rac and by sphingosine , is phosphorylated by PDK 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Analysis of small GTPase signaling pathways using p 21 activated kinase mutants that selectively couple to Cdc 42 . p 21 activated kinase 1 ( Pak 1 ) is an effector for the small GTPases Cdc 42 and Rac . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Serum induced the activity of Rac 1 and the activity or phosphorylation state of p 21 activated kinase 1 and c Jun NH ( 2 ) terminal kinase ( JNK ) , two intracellular targets of Rac 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| PAK 1 ( p 21 activated kinase 1 ) activation is induced by C albicans , suggesting that PAK 1 may also be involved in the Rac 1 activation of MAPK / ERK . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The ephrinA 1 mediated morphological alterations correlated with inhibition of Rac 1 and p 21 activated kinase 1 ( PAK 1 ) activity , and were antagonized by the expression of a constitutively active Rac mutant . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Here , we show that KSHV GPCR transformation requires activation of the small G protein Rac 1 and its effector , the p 21 activated kinase 1 ( Pak 1 ) . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Gbetagamma subunits stimulate p 21 activated kinase 1 ( PAK 1 ) through activation of PI 3 kinase and Akt but act independently of Rac1 / Cdc42 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| We investigated the role of Rac and p 21 activated kinase 1 ( PAK 1 ) in the regulation of myosin 2 B in prostate cancer cells in response to epidermal growth factor ( EGF ) stimulation . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that myosin light chain ( MLC ) phosphorylation by MLC kinase is regulated during E . coli invasion by an upstream kinase , p 21 activated kinase 1 ( PAK 1 ) , which is an effector protein of Rac and Cdc 42 GTPases , but not of RhoA . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Rac 1 is distinct from RhoA in its ability to bind and activate the p 65 PAK serine / threonine kinase , to induce lamellipodia and membrane ruffling , and to activate the c Jun NH 2 terminal kinase ( JNK ) . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Although neither activated Rac nor the PAKs stimulate ERK 2 activity , overexpression of either dominant negative Rac 2 or the N terminal regulatory domain of PAK 1 inhibits Ras mediated activation of ERK 2 , suggesting a permissive role for Rac in the control of the ERK pathway . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , because Rac 1 and Cdc 42 but not Rho bind and activate a kinase known as Pak 1 , it has been suggested that Pak 1 is the most upstream component of the pathway linking these GTPases to JNK . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Pak 1 ( L 83 , L 86 , R 299 ) , a mutant that fails to bind either Rac or Cdc 42 , also inhibited Ras transformation . ^^^ Rac and Ras activation of JNK was inhibited by Pak 1 ( R 299 ) but not by Pak 1 ( L 83 , L 86 , R 299 ) . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| PAK 1 was also colocalized with F actin in membrane ruffles extended as a response to constitutive activation of Rac 1 . ^^^ These data indicate a close correlation between the subcellular distribution of endogenous PAK 1 and the formation of Rac / Cdc42 dependent cytoskeletal structures and support an active role for PAK 1 in regulating cortical actin rearrangements . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The prolonged overexpression of Pak 1 amino terminal mutants that are unable to bind Cdc 42 or Rac 1 results in the accumulation of filamentous actin in large , polarized membrane ruffles and the formation of vinculin containing focal complexes within these structures . ^^^ CONCLUSIONS : These results indicate that Pak 1 , acting through a protein that contains an SH 3 domain , regulates the structure of the actin cytoskeleton in mammalian cells , and may serve as an effector for Cdc 42 and / or Rac 1 in promoting cell motility . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Coexpression studies in COS 7 cells revealed that the ability of individual guanine nucleotide exchange factors ( GEFs ) to activate the p 21 activated kinase PAK 1 could be dissociated from activation of c Jun amino terminal kinase , even though activation of both pathways requires the action of the GEFs on Rac and / or Cdc 42 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| This report shows that Rac 1 binds to and stimulates the kinase activity of PAK 1 approximately 2 and 4 5 fold , respectively , better than Rac 2 . ^^^ Mutation of these six basic residues in Rac 1 to neutral amino acids dramatically decreased the ability of Rac 1 to bind PAK 1 and almost completely abolished its ability to stimulate PAK activity . ^^^ Mutation of these Lys residues to neutral residues decreased PAK binding to activated Rac 1 and Rac 2 ( but not Cdc 42 ) and greatly reduced PAK 1 activation by Rac 1 , Rac 2 , and Cdc 42 proteins in vivo . ^^^ In contrast , mutation of lysines 66 68 to basic Arg residues did not decrease ( and in some cases enhanced ) the ability of Rac 1 , Rac 2 , and Cdc 42 to bind and activate PAK 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The p35 / Cdk5 kinase is a neuron specific Rac effector that inhibits Pak 1 activity . ^^^ Another Rac effector , Pak 1 kinase , is also present in the Rac p35 / Cdk5 complexes and co localizes with p35 / Cdk5 and Rac at neuronal peripheries . ^^^ The active p35 / Cdk5 kinase causes Pak 1 hyperphosphorylation in a Rac dependent manner , which results in down regulation of Pak 1 kinase activity . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| However , the Pak 1 ( H 83 , 86L ) mutant that does not bind Rac or Cdc 42 is activated in the absence of GTPase by alphaPix 155 545 and by a mutant of alphaPix 155 545 that no longer has exchange factor activity . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of LIM kinase by Pak 1 couples Rac / Cdc42 GTPase signalling to actin cytoskeletal dynamics . ^^^ In vivo , activated Rac or Cdc 42 increases association of Pak 1 with LIM kinase ; this association requires structural determinants in both the amino terminal regulatory and the carboxy terminal catalytic domains of Pak 1 . ^^^ A catalytically inactive LIM kinase interferes with Rac , Cdc 42 and Pak 1 dependent cytoskeletal changes . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Introducing dominant negative Rac 3 and Pak 1 fragments into a breast cancer cell line revealed that active Rac 3 drives Pak and JNK kinase activities by two separate pathways . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Together with the recent finding that PAK 1 , a downstream effector of Rac , also activates LIMK 1 by phosphorylation at Thr 508 , these results suggest that activation of LIMK 1 is one of the common targets for Rho and Rac to reorganize the actin cytoskeleton . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| In addition , we identified some of the upstream events accompanying the beta 1 integrin mediated p 38 MAPK activation , namely , the activation of the Rac guanine nucleotide exchange factor ( GEF ) p 95 Vav , the small G protein Rac 1 , and the cytoplasmic kinases Pak 1 and MKK 3 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The p 21 activated kinase ( PAK 1 ) is a serine threonine protein kinase that is activated by binding to the Rho family small G proteins Rac and Cdc42hs . ^^^ Here we show that expression of active Ras , Raf 1 , or Rac 1 in fibroblasts stimulates NFkappaB in a PAK 1 dependent manner and that expression of active PAK 1 can stimulate NFkappaB on its own . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Temporal and spatial distribution of activated Pak 1 in fibroblasts . p 21 activated kinases ( Paks ) are effectors of the small GTPases Cdc 42 and Rac , and are thought to mediate some of the cytoskeletal and transcriptional activities of these proteins . ^^^ Immunofluorescence analysis of NIH 3T3 cells coexpressing activated Cdc 42 or Rac 1 plus wild type Pak 1 shows that activated Pak 1 accumulates at sites of focal adhesion , throughout filopodia and within the body and edges of lamellipodia . ^^^ Platelet derived growth factor stimulation of NIH 3T3 cells shows a pattern of Pak 1 activation similar to that observed with Rac 1 . ^^^ These findings indicate that activated Pak 1 , and by extension , probably activated Cdc 42 or Rac , accumulates at sites of cortical actin remodeling in motile fibroblasts . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Pak 1 and ROCK , respectively , activate LIMK 1 and LIMK 2 downstream of Rac and Rho ; however , an effector protein kinase for LIMKs downstream of Cdc 42 remains to be defined . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Interaction of hPIP 1 with PAK 1 inhibits the Cdc42 / Rac stimulated kinase activity through the N terminal regulatory domains of PAK 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , expression of a kinase inactive mutant of Pak or the N terminal inhibitory domain of Pak 1 can block the effect of Rac or Cdc 42 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Cotransfection of PAK 1 constructs containing mutations in both the kinase domain and the Cdc42 / Rac binding domain attenuated the PAK 1 mediated down regulation of the promoter , suggesting that Rac and Cdc 42 are required for PAK 1 mediated decreases in SR BI promoter activity . 5 ' Deletion analysis and gel shift data suggest that LPS inhibits binding of a novel transcription factor to a myeloid zing finger protein 1 like element ( 476 to 456 ) in the human SR BI promoter . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The small GTPase Rac and its effectors , the Pak 1 and p35 / Cdk5 kinases , have been assigned important roles in regulating cytoskeletal dynamics in neurons . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| PAK 1 is a protein kinase downstream of the small GTPases Rac and Cdc 42 that previous work has implicated in endothelial cell migration via modulation of cell contraction . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Akt , PAK 1 , and Rac 1 existed as cytosolic complex in resting PMN . ^^^ TNF alpha stimulation increased association of PAK 1 with Akt . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Here we report that even though Cdc42 / Rac1 or Akt are not activated , phosphatidylinositol 3 ( PI 3 ) kinase activation induces PAK 1 kinase activity . ^^^ Furthermore , PAK 1 was activated in cells expressing the dominant negative forms of Cdc 42 or Rac 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| AND 34 overexpression augments both autophosphorylation and kinase activity of the Cdc42 / Rac responsive serine / threonine kinase PAK 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The EGFR and a number of effector kinases ( mitogen activated protein extracellular signal regulated kinase kinase 1 and 2 , MAPK , Pak 1 , p 38 , c JunNH ( 2 ) terminal kinase and extracellular signal regulated kinase 1 ) and cell survival proteins ( AKT , FKHR , and c Src ) showed constitutive pathway activation in HaCaT and cutaneous squamous cell carcinoma cells . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Cleavage of Rac 1 occurs at two unconventional caspase 3 sites , VVGD11 / G and VMVD47 / G , and results in inactivation of the GTPase and effector functions of the protein ( binding to the p 21 activated protein kinase PAK 1 ) . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The serine / threonine kinase Pak 1 is a target of the RhoGTPases Rac and Cdc 42 and an important regulator of cell morphology and migration . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The data revealed decreased Rac 1 activity within 4 h , and a decrease in the Rac 1 effector , PAK 1 , within 12 h . ^^^ These data suggest that maspin may inhibit cell motility by regulating Rac 1 and subsequently PAK 1 activity , and promote cell adhesion via PI3K / ERK pathways . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Like PAK 4 , PAK 6 possesses a constitutive basal kinase activity that , unlike PAK 1 , is not modulated by the binding of active Rac or Cdc 42 GTPases . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Dominant negative Ras , Rac , and Cdc 42 inhibited PAK 1 activation by all of these agonists , while active Rac 1 and Cdc 42 were sufficient to maximally activate PAK 1 in the absence of any treatment . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| AND 34 / BCAR3 , a GDP exchange factor whose overexpression confers antiestrogen resistance , activates Rac , PAK 1 , and the cyclin D 1 promoter . ^^^ Consistent with these findings , BCAR 3 overexpression induced alterations in F actin distribution and augmented both autophosphorylation and kinase activity of the Cdc42 / Rac responsive serine / threonine kinase PAK 1 . p130Cas associated BCAR 3 protein was detected in the estrogen independent breast cancer cell line 578 T , but not in estrogen dependent MCF 7 or ZR 75 1 cells . ^^^ Such cyclin D 1 promoter activation was inhibited by dominant negative forms of Rac 1 and PAK 1 . ^^^ These studies suggest that AND 34 / BCAR3 induces antiestrogen resistance in breast cancer cell lines by a Rac 1 and PAK 1 dependent pathway . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| We identify GEF H 1 as a binding target and substrate for p 21 activated kinase 1 ( PAK 1 ) , an effector of Rac and Cdc 42 GTPases , using an affinity based screen and localize a PAK 1 phosphorylation site to the inhibitory carboxyl terminal region of GEF H 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of the Src and Rac effector p 21 activated protein kinase ( PAK ) 1 on Thr 423 ( the phosphoinositide dependent kinase 1 [ PDK 1 ] site ) was attenuated by ROS inhibition , and infection of VSMCs with dominant negative PAK 1 adenovirus attenuated migration . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| In vitro it could effectively inhibit Rac 1 binding and activation by the Rac specific GEF Trio or Tiam 1 in a dose dependent manner without interfering with the closely related Cdc 42 or RhoA binding or activation by their respective GEFs or with Rac 1 interaction with BcrGAP or effector PAK 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Rac 1 and PAK 1 are upstream of IKK epsilon and TBK 1 in the viral activation of interferon regulatory factor 3 . ^^^ As a downstream mediator of Rac signaling towards IRF 3 , we have identified the kinase p 21 activated kinase ( PAK 1 ) . ^^^ Furthermore , both Rac 1 and PAK 1 regulate the recently described IRF 3 activators , 1 kappa B kinase and TANK binding kinase 1 , establishing a first canonical virus induced IRF 3 activating pathway . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of RhoGDI by Pak 1 mediates dissociation of Rac GTPase . ^^^ We observed that Cdc 42 induced Rac 1 activation is inhibited by expression of Pak 1 autoinhibitory domain . ^^^ The dissociation of Rac 1 from RhoGDI and its subsequent activation stimulated by PDGF or EGF is also attenuated by Pak 1 autoinhibitory domain , and this is dependent on the ability of RhoGDI to be phosphorylated at Ser101 / 174 . ^^^ These results support a role for Pak 1 mediated RhoGDI phosphorylation as a mechanism for Cdc 42 mediated Rac activation , and suggest the possibility of Rac induced positive feed forward regulation of Rac activity . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Analysis of the activation state of the components upstream of p 38 and JNK showed that NSAIDs inhibit the serine threonine kinase p 21 activated protein kinase 1 ( Pak 1 ) and the small guanosine 5 ' triphosphatase ( GTPase ) Rac , as well as the Rac specific guanine nucleotide exchanger , Vav . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the synergistic activation of Erk was inhibited by expression of a dominant negative mutant of Pak 1 or that of Rac and was enhanced by an activated mutant of Pak 1 . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The ability of HGF / SF to activate NF kappaB signaling was dependent on c Akt > Pak 1 ( p 21 associated kinase 1 ) signaling ( with Pak 1 downstream of c Akt ) and was inhibited by the tumor suppressor PTEN ( phosphatase and tensin homolog ) . ^^^ These findings suggest that HGF / SF activates NF kappaB through a c Akt > Pak 1 signaling pathway that is also dependent on Src , and that NF kappaB contributes to HGF / SF mediated protection against ADR . . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| The ability of PAK 1 to maintain neuronal survival is , however , blocked by ML 9 , a compound known to inhibit Akt . ^^^ Our results show that that PAK 1 is necessary for neuronal survival in HK and suggest that its neuroprotective action may be mediated by a GTPase independent , but Akt dependent , mechanism . . ^^^ Although PAK 1 is usually considered to be a downstream effector of Rac and Cdc 42 , we were unable to detect association between PAK 1 and either Rac 1 or Cdc 42 in cerebellar granule neurons . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of Rac 1 was measured in response to agents stimulating H2O2 production , using the CRIB domain of PAK 1 as a probe in a glutathione S transferase ( GST ) pull down assay . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Thrombin and active PAKT423E phosphorylated p 38 MAP kinase ( p38MAPK ) , ERK1 / 2 , phosphatidylinositol dependent kinase 1 ( PDK 1 ) and protein kinase B / Akt ( PKB ) whereas kinase deficient PAK 1 prevented activation of these kinases by thrombin . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Nischarin regulates Rac 1 dependent cell motility by interaction with and inhibition of the p 21 activated kinase ( PAK 1 ) . ^^^ In addition to regulating the activation of PAK 1 , Rac 1 controls multiple downstream pathways to regulate cell growth and differentiation , as well as cell motility . ^^^ |
|
| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Endogenous Akt , PAK 1 , and BAD are phosphorylated in attached MCF10A cells , but these phosphorylation events are all lost during the first 8 hours of detachment . ^^^ Expression of constitutively active PAK 1 or Akt suppresses the cleavage of caspase 3 and PARP in detached MCF10A cells . ^^^ Co overexpression of active PAK 1 with dominant negative Akt , or of active Akt with dominant negative PAK 1 , still suppresses anoikis . ^^^ Thus , Akt and PAK 1 enhance survival through pathways that are at least partially independent . ^^^ |
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| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| In addition , dnPYK 2 , dnRac , and antioxidants inhibited ANG 2 induced PAK 1 phosphorylation , suggesting that PYK 2 , Rac , and reactive oxygen species are involved in the upstream signaling . ^^^ |
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| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , ALS 2 stimulates Rac but not Rho or Cdc 42 activities , and this induces a corresponding increase in PAK 1 activity . ^^^ |
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| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Overexpression of antioxidants ( superoxide dismutase and catalase ) and Rac1N17 , as well as preincubation with selective inhibitors of NADPH oxidase augmented basal p 38 phosphorylation , inhibited Ang 1 induced PAK 1 phosphorylation and potentiated Ang 1 induced Erk1 / 2 phosphorylation but had no influence on AKT and SAPK / JNK phosphorylation by Ang 1 . ^^^ |
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| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Although Rac 1 deletion caused a significant reduction in phospho PAK 1 , AKT , and ERK under serum stimulation , reconstitution of active PAK 1 , but not AKT or MEK 1 , was able to rescue the actin cytoskeleton and adhesion phenotypes of the Rac 1 deficient cells . ^^^ Furthermore , Rac 1 deletion led to a marked increase in spontaneous apoptosis that could be rescued by active PAK 1 , AKT , or MEK 1 expression . ^^^ |
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| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| A Dictyostelium PAK ( PAKa ) acts indirectly to promote myosin 2 filament formation , suggesting that the MHCKs may be indirectly regulated by Rac GTPases . . ^^^ |
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| Interacting proteins: Q13153 and P31749 |
Pubmed |
SVM Score :0.0 |
| Control of cell polarity and chemotaxis by Akt / PKB and PI 3 kinase through the regulation of PAKa . ^^^ We demonstrate that PI 3 kinase and protein kinase B ( PKB or Akt ) control cell polarity and chemotaxis , in part , through the regulation of PAKa , which is required for myosin 2 assembly . ^^^ We demonstrate that PI3K and PKB mediate PAKa ' s subcellular localization , PAKa ' s activation in response to chemoattractant stimulation , and chemoattractant mediated myosin 2 assembly . ^^^ Mutation of the PKB phosphorylation site in PAKa to Ala blocks PAKa ' s activation and inhibits PAKa redistribution in response to chemoattractant stimulation , whereas an Asp substitution leads to an activated protein . ^^^ |
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