| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.63755763 |
| Here , we demonstrate that Akt , including AKT 1 and AKT 2 , interacts with and phosphorylates 10 linked inhibitor of apoptosis protein ( XIAP ) at residue serine 87 in vitro and in vivo . 0.63755763^^^ |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Role of 10 linked inhibitor of apoptosis protein in chemoresistance in ovarian cancer : possible involvement of the phosphoinositide 3 kinase / Akt pathway . ^^^ |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Induction of apoptosis was also accompanied by release of cytochrome c , down regulation of 10 linked inhibitor of apoptosis protein ( XIAP ) , and inactivation of Akt , which was blocked by the pan caspase inhibitor z VAD fmk . ^^^ |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Moreover , TGF beta 1 reduced both Akt ( a well known survival factor ) phosphorylation and XIAP ( 10 linked inhibitor of apoptosis protein ) expression in decidual endometrial stromal cells in vitro . ^^^ |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| XIAP regulates Akt activity and caspase 3 dependent cleavage during cisplatin induced apoptosis in human ovarian epithelial cancer cells . ^^^ In the present study , we have investigated the role of XIAP in the regulation of the PI 3 K / Akt survival pathway in chemosensitive ( A 2780 s , OV 2008 , and OVCAR 3 ) and resistant ( A 2780 cp ) ovarian cancer cell lines and the nature of this interaction in cell death / survival signaling . ^^^ Cisplatin decreased XIAP protein levels and induced Akt cleavage and apoptosis in chemosensitive , but not in resistant , ovarian cancer cells . ^^^ Adenoviral sense XIAP cDNA expression increased XIAP protein levels and increased Akt phosphorylation , indicative of activation of Akt and , likely , of PI 3 K . ^^^ In a cell line ( OVCAR 3 ) where basal phosphorylated Akt levels were high , XIAP overexpression failed to increase further the level of this phosphoprotein . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| X linked inhibitor of apoptosis protein activates the phosphatidylinositol 3 kinase / Akt pathway in rat granulosa cells during follicular development . 10 linked inhibitor of apoptosis protein ( XIAP ) in granulosa cells is regulated by gonadotropins during follicular development , although the current understanding of the mechanisms by which XIAP suppressed granulosa cell apoptosis is incomplete . ^^^ Using a fully characterized in vivo model to study the induction of follicular development and atresia in immature rats , we have demonstrated that gonadotropin treatment increased granulosa cell XIAP and phospho Akt protein contents and suppressed apoptosis . ^^^ The increased apoptosis was accompanied by marked decreases in XIAP expression and phosphorylation of Akt protein . ^^^ Infection of granulosa cells from eCG primed rats with adenoviral sense XIAP [ lacZ as a control ; multiplicity of infection , 1 5 ] resulted in XIAP overexpression and increased phospho Akt content , whereas XIAP antisense expression ( multiplicity of infection , 10 40 ) decreased granulosa cell phospho Akt level and induced apoptosis . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Combined treatment with As2O3 and STI 571 also resulted in greater reductions in the levels of Bcl 10 ( L ) , XIAP and Akt , and inhibition of Akt kinase activity . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| IGF 1 activated Akt ; up regulated the caspase inhibitors FLIP , cIAP 2 , XIAP , and survivin ; and attenuated Apo2L / TRAIL induced apoptosis . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| In the current study , we demonstrate that IGF 1 stimulates sustained activation of NF kappaB and Akt ; induces phosphorylation of the FKHRL 1 Forkhead transcription factor ; upregulates a series of intracellular anti apoptotic proteins including FLIP , survivin , cIAP 2 , A1 / Bfl 1 , and XIAP ; and decreases Apo2L / TRAIL sensitivity of MM cells . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| The levels of Mcl 1 , Bax , Bid , Akt , c IAP 1 , c IAP 2 , XIAP and c FLIP were compared in all HL 60 lines . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| This effect is dependent upon the activation of the caspase system , inhibiting XIAP , an inhibitor of apoptosis , and disrupting FLICE inhibitory protein ( FLIP ) expression through the Akt signal transduction pathway . ^^^ |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| To test the possible involvement of Akt in the regulation of survival factors , Bcl 2 , XIAP , cIAP 1 and cIAP 2 expression were measured . cIAP 1 protein expression was high in cells expressing phospho Akt . ^^^ XIAP and cIAP 2 protein expression was not influenced by the presence of active Akt . ^^^ |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Neither these nor Akt inhibitor treated OCI AML 3 cells showed changes in XIAP levels , suggesting that XIAP expression is regulated by PI3K downstream effectors other than Akt . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , Xiap up regulates the phosphatidylinositol 3 ' kinase / Akt pathway by increasing Akt phosphorylation . ^^^ However , the precise relationships among Xiap , Akt , and p 53 in chemoresistance are unknown . ^^^ Here we show that both Xiap and Akt can modulate CDDP sensitivity individually but that Xiap requires Akt for its full function . ^^^ Expression of a constitutively active Akt 2 prevented CDDP mediated down regulation of Xiap and apoptosis in A2780s cells . ^^^ Akt 2 mediated chemoresistance could not be reversed by Xiap down regulation . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Reduced activation of Akt and NF kappaB and decreased induction of XIAP were observed in mature osteoclasts treated with SCOH . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Transcription of cIAP 2 and XIAP was up regulated by the phosphatidylinositol 3 kinase / Akt pathway as shown by its reversal by dominant negative Akt or LY 294002 . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Moreover , PI3K and Akt inhibitors , but not mammalian target of rapamycin inhibitor , inhibited the effect of insulin on the coprecipitation of procaspase 9 and XIAP . ^^^ Our data suggest that insulin decreases the TNF alpha induced cleavage of procaspase 9 and subsequent apoptosis by regulating XIAP via the PI3K / Akt pathway . . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| The effects of 2 Methoxyestradiol ( 2ME ) induced apoptosis was examined in human leukemia cells ( U 937 and Jurkat ) in relation to mitochondrial injury , oxidative damage , and perturbations in signaling pathways . 2ME induced apoptosis in these cells in a dose dependent manner associated with release of mitochondrial proteins ( cytochrome c , AIF ) , generation of reactive oxygen species ( ROS ) , downregulation of Mcl 1 and XIAP , and inactivation ( dephosphorylation ) of Akt accompanied by activation of JNK . ^^^ In these cells , enforced activation of Akt by a constitutively active myristolated Akt construct prevented 2ME mediated mitochondrial injury , XIAP and Mcl 1 downregulation , JNK activation , and apoptosis , but not ROS generation . ^^^ Collectively , these findings suggest a hierarchical model of 2ME related apoptosis induction in human leukemia cells in which 2ME induced oxidative injury represents a primary event resulting in Akt inactivation , leading , in turn , to JNK activation , and culminating in XIAP and Mcl 1 downregulation , mitochondrial injury , and apoptosis . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Similarly , XIAP , a target of AKT , was down regulated after LY 294002 treatment only in sensitive PEL cells . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| The role of HGF / C Met pathway through Akt and XIAP was investigated by small interfering RNA ( siRNA ) and quantitative RT PCR analysis of colorectal cancer lines . ^^^ Activation of XIAP expression by HGF was inhibited by siRNA targeting Akt 1 and Akt 2 . ^^^ CONCLUSIONS : Activation of C MET enhances XIAP through the Akt pathway . ^^^ The Akt XIAP pathway may be a potential molecular target for regulating colorectal cancer progression . . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| In addition , cleavage of XIAP and down regulation of Akt were observed . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with cerulenin and LY 294002 down regulated the protein levels of 10 chromosome linked inhibitor of apoptosis ( XIAP ) , cellular inhibitor of apoptosis 1 ( cIAP 1 ) , and Akt , whereas the levels of mitogen activated protein / extracellular signal regulated kinase kinase and other antiapoptotic Bcl 2 family proteins ( Bcl 2 and Bcl xl ) did not change . ^^^ Interestingly , the nonspecific caspase inhibitor , z VAD FMK , inhibited the down regulation of Akt , XIAP , and cIAP 1 in cerulenin and LY 294002 treated cells . ^^^ In conclusion , these studies show that inhibition of PI3K can sensitize cerulenin induced apoptosis in MBA MB 468 breast cancer cells via activation of caspases , down regulation of antiapoptotic proteins , such as XIAP , cIAP 1 and Akt , and possibly , activation of Bak in mitochondria . . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| The proteasome inhibitor MG 132 blocked FD mediated reduction of XIAP and AKT and antagonized apoptosis , suggesting that the activation of the proteasome pathway is one of the mechanisms involved . ^^^ Overall , our data suggest that the docetaxel and flavopiridol combination requires a maximal effect on cyclin B 1 dependent kinase activity and a reduction of XIAP and AKT prosurvival proteins for augmentation of apoptosis in LNCaP cells . . ^^^ |
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| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| Expression of survivin , 10 chromosome linked IAP ( XIAP ) , Bcl xL , AKT and phospho AKT was also investigated using immunoblotting . ^^^ |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P98170 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|