| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| The inhibitory effect of the cyclin dependent kinase inhibitors , p 16 ( ink 4 ) , p 21 ( cip 1 ) , and p 27 ( cip ) on Rac / Cdc42 mediated E2F transcription corroborates a role for pRB family members and their functional inactivation by cyclin dependent kinases in generating E2F activity . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Akt dependent phosphorylation of p 21 ( Cip 1 ) regulates PCNA binding and proliferation of endothelial cells . ^^^ Here , we demonstrate that Akt phosphorylates the cell cycle inhibitory protein p 21 ( Cip 1 ) at Thr 145 in vitro and in intact cells as shown by in vitro kinase assays , site directed mutagenesis , and phospho peptide analysis . ^^^ Akt dependent phosphorylation of p 21 ( Cip 1 ) at Thr 145 prevents the complex formation of p 21 ( Cip 1 ) with PCNA , which inhibits DNA replication . ^^^ These data suggest that the modulation of p 21 ( Cip 1 ) cell cycle functions by Akt mediated phosphorylation regulates endothelial cell proliferation in response to stimuli that activate Akt . . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Signaling via the phosphoinositide 3 kinase ( PI3K ) / AKT pathway is crucial for the regulation of endothelial cell ( EC ) proliferation and survival , which involves the AKT dependent phosphorylation of the DNA repair protein p 21 ( Cip 1 ) at Thr 145 . ^^^ Because p 21 ( Cip 1 ) is a short lived protein with a high proteasomal degradation rate , we investigated the regulation of p 21 ( Cip 1 ) protein levels by PI3K / AKT dependent signaling . ^^^ We therefore investigated whether a kinase downstream of AKT regulates p 21 ( Cip 1 ) protein levels . ^^^ In contrast , stimulation of AKT increases p 21 ( Cip 1 ) via inhibitory phosphorylation of GSK 3 . . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| In contrast , FR 901228 induces p 21 ( Cip 1 ) expression in nontransformed counterpart cultures growth arrested in G0 / G1 that is also accompanied by moderate induction of the kinase activities of Raf , Mek , Erk , and Akt , but not accompanied by activation of caspase 3 or changes in p 38 activity . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of Raf led to nuclear accumulation of p 21 ( Cip 1 ) , and we provide evidence that this effect is mediated by inhibition of Akt , a regulator of p 21 ( Cip 1 ) localization . ^^^ In agreement , expression of E 7 sustained Akt activity and reduced nuclear accumulation of p 21 ( Cip 1 ) , resulting in decreased association between p 21 ( Cip 1 ) and cyclin E CDK 2 . ^^^ Taken together , these data suggest that E 7 inhibits p 21 ( Cip 1 ) function in the context of Raf signaling by altering Raf Akt antagonism and preventing the proper subcellular localization of p 21 ( Cip 1 ) . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : S phase delay induced by overexpression of integrin beta 1 subunit is attributed to the decrease of PKB phosphorylation and subsequent increases of p 21 ( cip 1 ) and p 27 ( kip 1 ) proteins , and may be involved in the unoccupied alpha5beta1 because of lack of its ligands . . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| RhoE expressing cells failed to accumulate cyclin D 1 or p 21 ( cip 1 ) protein or to activate E2F regulated genes in response to serum , although ERK , PI 3 K / Akt , FAK , Rac , and cyclin D 1 transcription was activated normally . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| These effects did not depend on the Akt substrate p 21 ( Cip 1 ) . ^^^ The ability of elevated Akt 1 to increase the self renewing population therefore depends on a rapamycin sensitive mechanism ( presumably inhibition of mTOR activity ) but not on p 21 ( Cip 1 ) , and can be distinguished from its effects on the proliferation and survival of other types of progenitors . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we demonstrated that AKT activation led to sustained activation of cyclin / cdk2 complexes that occurred concomitantly with the removal of RAF induced p 21 ( Cip 1 ) from cyclin E / cdk2 complexes . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Immunobiot analyses indicated that G 1 arrest induced by the subapoptogenic doses of MSeA was associated with increased expression of p27kip1 and p21cip1 , but apoptosis was accompanied by dose dependent decreases of phosphorylation of protein kinase AKT and extracellular signal regulated kinase ( ERK1 / 2 ) in the absence of any phosphorylation change in p 38 mitogen activated protein kinase ( p38MAPK ) and c Jun NH 2 terminal kinase ( JNK1 / 2 ) . ^^^ In contrast , selenite exposure caused S phase arrest and caspase independent apoptotic DNA fragmentation , which were associated with decreased expression of p27kip1 and p21cip1 and increased phosphorylation of AKT , JNK1 / 2 , and p38MAPK . ^^^ Taken together , exposure of DU 145 cells to MSeA versus selenite induced differential patterns of cell cycle arrest and apoptosis execution as well as distinct patterns of effects on AKT , ERK1 / 2 , JNK1 / 2 , and p38MAPK phosphorylation and p27kip1 and p21cip1 expression . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| These effects were at least in part modulated , both in vitro and in vivo , by increased p21Cip1 expression , as demonstrated by lack of effect of AA Akt on cell proliferation in p 21 / mouse SMCs . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| These events were associated with a marked increase in ROS generation , inactivation of ERK and Akt , downregulation of p21CIP1 , Bcr / Abl , and cyclin D 1 , and activation of JNK . ^^^ Using pharmacologic and genetic approaches , generation of ROS , p21CIP1 downregulation , and inactivation of Akt and MEK were found to play significant functional roles in SAHA / PD184352 mediated lethality , whereas JNK activation and Raf 1 downregulation were determined to represent secondary events . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Accompanying this , the expressions of CDKs and p 21 ( WAF 1 ) were down and up regulated , respectively , in both parental PC 12 cells and cells expressing mutant Akt . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Whereas the PI 3K / Akt pathway is constitutively activated in these cells , inactivation of this pathway restores the loading of CBP and the RNA polymerase and the expression of the p 21 ( waf 1 ) gene without having any effect on myc regulation . ^^^ Together , these findings suggest that the phosphatidylinositol 3 kinase / Akt pathway inhibits the transcriptional activation of the p 21 ( waf 1 ) gene by STAT 3 proteins without altering the regulation of the myc promoter . . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| We show that PTEN reconstitution diminished phosphorylation of AKT , induced the transactivation of p 53 ( 7 . 5 fold induction ) and increased the expression of p 53 target genes , p 21 ( waf 1 ) and insulin like growth factor binding protein 3 in glioma cells . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Apoptotic changes were increased , DNA synthesis was decreased , and the expressions of FAS , neu , Akt , phospho Akt , and p 21 ( waf 1 ) were all decreased when compared to vehicle controls and FVB / N mice . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Examined apoptosis related genes included tumor suppressor genes p 53 , p 53 ; mouse double minute 2 , MDM 2 ; cyclin dependent kinase inhibitor p 21 ( WAF 1 ) , p 21 ( waf ) ; Bcl 2 associated protein 10 , BAX ; apoptotic protease activating factor 1 , Apaf 1 ; Caspase 9 and serine / threonine protein kinase , PKB . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Following this , apoptosis and the levels of p 21 ( WAF 1 ) , p 27 ( KIP 1 ) , AKT , c Raf , and Her 2 , as well as of the key regulators of apoptosis were determined . ^^^ |
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| Interacting proteins: P38936 and P31749 |
Pubmed |
SVM Score :0.0 |
| Ganglioside GM 3 modulates tumor suppressor PTEN mediated cell cycle progression transcriptional induction of p 21 ( WAF 1 ) and p 27 ( kip 1 ) by inhibition of PI 3K / AKT pathway . ^^^ Results from our current study show that GM 3 treatment dramatically increases cyclin dependent kinase ( CDK ) inhibitor ( CKI ) p 21 ( WAF 1 ) expression through the accumulation of p 53 protein by the PTEN mediated inhibition of the PI 3K / AKT / MDM2 survival signaling in HCT 116 colon cancer cells . ^^^ On the contrary , suppression of PTEN levels by RNA interference restores the enhanced expression of p 53 dependent p 21 ( WAF 1 ) and p 53 independent p 27 ( kip 1 ) through inactivating the effect of PTEN on PI 3K / AKT signaling modulated by ganglioside GM 3 . ^^^ |
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