| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| Overexpression of S1P2 sensitized , and that of either S1P1 or S1P3 desensitized , B 16 cells to S1P inhibition of Rac and migration . ^^^ In the presence of JTE 013 , S1P instead stimulated Rac and migration in B 16 cells that overexpress either S1P1 or S1P3 , unveiling counteractions between S1P2 and S1P1 or S1P3 chemotactic receptor . . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| Conversely , when overexpressed in PC 12 cells , transactivation of S1P1 by NGF markedly enhanced neurite extension and stimulation of the small GTPase Rac , important for the cytoskeletal changes required for neurite extension . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| We examined signaling pathways downstream of S1P1 and found that 100 nM S1P increased phosphorylation of Akt and decreased activation of c jun . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| These effects are mediated by G protein coupled receptors on the cell surface ( S1P1 5 ) , which activate downstream signaling intermediates such as Rac and Rho GTPases . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| Both S1P and the S1P1 specific agonist SEW 2871 induced Akt phosphorylation at Ser 473 . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| We also show that Edg 1 mediates an SPP induced migration response that is defective in mutant cells due to an inability to activate the small GTPase , Rac . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| Consistent with this , Edg 1 and Edg 3 , and Edg 5 regulate the activity of the Rho family GTPase Rac positively and negatively , respectively . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| Inhibitory regulation of Rac activation , membrane ruffling , and cell migration by the G protein coupled sphingosine 1 phosphate receptor EDG 5 but not EDG 1 or EDG 3 . ^^^ In CHO cells expressing EDG 1 or EDG 3 ( EDG 1 cells or EDG 3 cells , respectively ) S1P as well as insulin like growth factor 1 ( IGF 1 ) induced chemotaxis and membrane ruffling in phosphoinositide ( PI ) 3 kinase and Rac dependent manners . ^^^ In EDG 5 cells , S1P stimulated PI 3 kinase activity as it did in EDG 1 cells but inhibited the basal Rac activity and totally abolished IGF 1 induced Rac activation , which involved stimulation of Rac GTPase activating protein activity rather than inhibition of Rac guanine nucleotide exchange activity . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| Akt mediated phosphorylation of the G protein coupled receptor EDG 1 is required for endothelial cell chemotaxis . ^^^ Here we show that sphingosine 1 phosphate ( S1P ) induced endothelial cell migration requires the Akt mediated phosphorylation of the G protein coupled receptor ( GPCR ) EDG 1 . ^^^ Activated Akt binds to EDG 1 and phosphorylates the third intracellular loop at the T ( 236 ) residue . ^^^ Transactivation of EDG 1 by Akt is not required for G ( 1 ) dependent signaling but is indispensable for Rac activation , cortical actin assembly , and chemotaxis . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| We found that fibroblasts from EDG 1 null embryos did not migrate toward PDGF or SPP , and inhibition of motility correlated with defective activation of the small guanosine triphosphatase Rac , which is required for lamellipodia formation and directional locomotion [ Hobson , Rosenfeldt , Barak , Olivera , Poulton , Caron , Milstien , and Spiegel ( 2001 ) Science 291 , 1800 1803 ] . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| Overexpression of Edg 1 in SMCs and Edg 5 in HUVECs reduced S1P induced inhibition and stimulation , respectively , of Rac activity and migration . ^^^ |
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| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| In contrast to the cellular responses elicited by S1P in COS 7 cells , LPA can stimulate the activation of eNOS and Akt independently of EDG 1 receptor transfection . ^^^ Taken together , these results indicate that LPA potently activates eNOS in BAEC in a pathway distinct from the EDG 1 receptor , but mediated by a similar receptor mediated pathway dependent on pertussis toxin sensitive G proteins and involving activation of the PI 3 K / Akt pathway . ^^^ |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P21453 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|