| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.5089943 |
| The selective substrate specificity is correlated with an enhanced association between Akt or SGK and their preferred substrates , FKHR and B Raf , respectively . 0.5089943^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Negative regulation of the serine / threonine kinase B Raf by Akt . ^^^ B Raf contains multiple Akt consensus sites located within its amino terminal regulatory domain . ^^^ We show that phosphorylation of B Raf by Akt occurs at multiple residues within its amino terminal regulatory domain , at both the conserved and unique phosphorylation sites . ^^^ Furthermore , expression of Akt inhibits epidermal growth factor induced B Raf activity and inhibition of Akt with LY 294002 up regulates B Raf activity , suggesting that Akt negatively regulates B Raf in vivo . ^^^ Our results demonstrate that B Raf activity can be negatively regulated by Akt through phosphorylation in the amino terminal regulatory domain of B Raf . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| The in vitro phosphorylation of a site unique to B Raf ( Ser 429 ) has been proposed to be responsible for the negative regulation of the isoenzyme by Akt . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , a Src mutant ( SrcS17D ) , which mimics PKA phosphorylation at serine 17 , stimulates Rap 1 activation , Rap1 / B Raf association , and ERK activation but does not stimulate Ras or AKT . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| In this study , the effects of activated Raf ( Raf 1 , A Raf and B Raf ) and PI3K / Akt proteins on abrogation of cytokine dependence in FL5 . 12 hematopoietic cells were examined . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Moreover , the activity of Akt , a negative regulator of B Raf , was decreased by calcium restriction . ^^^ Inhibition of Akt or phosphatidylinositol 3 kinase also allowed cAMP dependent activation of B Raf and ERK in normal calcium . ^^^ These results suggest that calcium restriction causes an inhibition of the phosphatidylinositol 3 kinase / Akt pathway , which relieves the inhibition of B Raf to allow the cAMP growth stimulated phenotypic switch . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| In microcystin transformed cells , PI3K , MAPKAPK 2 , Akt , cyclin D 1 and cyclin D 3 in the Akt pathway ; IQGAP 2 , RabGTPase , Rap1GAP , RasGAP , R Ras , Krev 1 and TC 21 of the Ras GTP / GDP protein family ; and A Raf , B Raf and PAK in the Ras / MAPK pathway were all markedly upregulated . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Functional consequences of mutations in a putative Akt phosphorylation motif of B raf in human cancers . ^^^ Some non V599E mutations have been found surrounding Thr 439 , which is thought likely to be one of the three Akt phosphorylation sites in the B raf protein . ^^^ The inhibition of phosphatidylinositol 3 kinase ( PI3K ) / Akt signaling by LY 294002 increased the Erk activation induced by the mutant B raf proteins , as well as by wild type B raf . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Curcumin induced antiproliferative and proapoptotic effects in melanoma cells are associated with suppression of IkappaB kinase and nuclear factor kappaB activity and are independent of the B Raf / mitogen activated / extracellular signal regulated protein kinase pathway and the Akt pathway . ^^^ In addition , relevant signaling , as reflected by B Raf kinase activity ( kinase cascade assay ) , and steady state levels of activated , downstream effectors , as reflected by mitogen activated signal regulated protein kinase ( MEK ) , extracellular signal regulated protein kinase ( ERK ) , and Akt phosphorylation levels ( immunoblots ) , were assessed . ^^^ However , curcumin did not inhibit the activities of B Raf , MEK , or ERK , and Akt phosphorylation was enhanced . ^^^ These effects were associated with the suppression of NF kappaB and IKK activities but were independent of the B Raf / MEK / ERK and Akt pathways . . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Bag 1 is a cochaperone for the heat shock protein Hsp 70 that interacts with C Raf , B Raf , Akt , Bcl 2 , steroid hormone receptors and other proteins . ^^^ However , the defect was associated with the disturbance of a tripartite complex formed by Akt , B Raf and Bag 1 , in addition to the absence of Bad phosphorylation at Ser 136 . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| We obtained paraffin tissue blocks of primary tumors from 31 patients , sequenced the EGFR , KRAS , and BRAF genes , and did immunohistochemistry for EGFR , phosphorylated AKT 1 , p 53 , p 21 , and p 27 . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Elevation of intracellular Ca2+ levels in ADPKD cells increased Akt activity and blocked cAMP dependent B Raf and ERK activation . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| BCR / ABL and IL 3 activate Rap 1 to stimulate the B Raf / MEK / Erk and Akt signaling pathways and to regulate proliferation , apoptosis , and adhesion . ^^^ Here , we demonstrate that the abrogation of Rap 1 activation by SPA 1 , a Rap 1 specific GAP , inhibits activation of B Raf , MEK , Erk , and Akt in a murine hematopoietic cell line , Ton . ^^^ Thus , IL 3 or BCR / ABL induced activation of Rap 1 may play important roles in regulation of cell proliferation and survival through activation of the B Raf / MEK / Erk and Akt signaling pathways and in induction of integrin mediated cell adhesion . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| Hsp 90 client proteins include telomerase , mutated p 53 , Bcr Abl , Raf 1 , Akt , HER2 / Neu ( ErbB 2 ) , mutated B Raf , mutated EGF receptor , and HIF 1alpha . ^^^ |
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| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| METHODS : Biomarkers included epidermal growth factor receptor ( EGFR ) gene copy number by fluorescence in situ hybridization ( n = 370 ) ; EGFR ( n = 379 ) and phosphorylated Akt ( p Akt ) protein expression ( n = 382 ) by immunohistochemistry ; and mutations in EGFR ( n = 215 ) , KRAS ( n = 152 ) , and BRAF ( n = 118 ) . ^^^ No relationship was observed between p Akt protein expression and survival outcome , and the limited amount of data collected for KRAS and BRAF mutations prevented any meaningful evaluation of clinical outcomes in relation to these mutations . ^^^ |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P15056 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|