| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| EC 50 values for neither p 47 phox nor p 67 phox were affected when mutant Racs were used in place of Rac . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Constitutive activation of protein kinase B and phosphorylation of p47phox by a membrane targeted phosphoinositide 3 kinase . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Modulation of p47PHOX activity by site specific phosphorylation : Akt dependent activation of the NADPH oxidase . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Dissociation of Rac translocation from p47phox / p67phox movements in human neutrophils by tyrosine kinase inhibitors . ^^^ Cytosolic components of the phagocyte NADPH oxidase ( p47phox , p67phox , and Rac 2 ) translocate to the plasma membrane on cell activation where they interact with a membrane bound cytochrome b to generate superoxide anion . ^^^ Translocation of Rac 2 , p47phox , and p67phox were all enhanced in formyl Met Leu Phe stimulated neutrophils treated with 50 nM of the protein phosphatase 1 / 2A inhibitor calyculin A . ^^^ Rac translocation was blocked by the tyrosine kinase inhibitors genistein ( 50 microM ) and herbimycin ( 17 microM ) , whereas movement of p47phox and p67phox were not inhibited . ^^^ Cell free analysis of Rac translocation also demonstrated that translocation of p47phox and p67phox were not linked to the movement or availability of Rac 2 . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Native and recombinant PAKs phosphorylated the p47phox reduced nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase component in a Rac GTP dependent manner . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Production of microbicidal oxidants by phagocytic leukocytes requires activation of a latent NADPH oxidase by the coordinated assembly of a membrane associated flavocytochrome b 558 , with three cytosolic components , p47phox , p67phox , and the low molecular weight GTP binding protein Rac . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| In the present studies , we have used a semirecombinant system consisting of recombinant cytosolic factors ( p 47 phox , p 67 phox , and Rac 1 ) along with isolated plasma membrane to investigate the mechanism by which the peptide inhibits oxidase activation . ^^^ Inhibition was noncompetitive or mixed with respect to not only Rac and p 67 phox but also to p 47 phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The superoxide generating NADPH oxidase complex in phagocytic cells is constituted of a heterodimeric flavocytochrome b and cytosolic factors , p67phox , p47phox and p40phox as well as a small G protein Rac ( for review , see Refs . 1 3 ) . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| We also found that Rac 1 interacts with two other NADPH oxidase components , p67phox and p47phox , upon monocyte activation . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Rac translocates independently of the neutrophil NADPH oxidase components p47phox and p67phox . ^^^ When the neutrophil NADPH oxidase is activated to generate superoxide , the cytosolic components , p47phox , p67phox , and the GTP binding protein Rac , become stably associated with the plasma membrane . ^^^ When cell free reactions contained any combination of normal , p47phox deficient or p67phox deficient cytosol and normal or flavocytochrome b 558 deficient membranes , the GTP gamma S ( guanosine 5 ' 3 O ( thio ) triphosphate ) dependent association of Rac 2 with the re isolated membranes was not significantly different from the control mixture of normal membranes and cytosol . ^^^ In intact CGD neutrophils lacking p47phox or p67phox and stimulated with phorbol myristate acetate the translocation of Rac 2 was also normal , and we conclude that Rac translocation can occur independently of the cytosolic NADPH oxidase components . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| These components include a membrane NADPH binding flavoprotein , cytochrome b 558 , the cytosolic proteins p47phox , p67phox and a small ras related guanosine triphosphatase or rac protein that confers guanosine triphosphate sensitivity to the NADPH oxidase . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NADPH dependent superoxide generation was activated by anionic amphiphiles plus GTP gamma S in a cell free system consisting of plasma membranes plus recombinant p 47 phox , p 67 phox , and the small GTP binding protein Rac 1 . ^^^ The peptide inhibited competitively ( Ki = 15 microM ) with respect to Rac 1 ( C189S ) , while inhibition was noncompetitive or mixed with respect to p 47 phox and p 67 phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| We directly compared recombinant Rac 1 and Rac 2 in a human neutrophil cell free NADPH oxidase system in which cytosol was replaced by purified recombinant cytosolic components ( p 47 phox and p 67 phox ) . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The superoxide generating NADPH oxidase system in phagocytes consists of membrane associated cytochrome b 558 and three cytosolic components named p 67 phox , p 47 phox , and rac p21s . ^^^ Incubation of cytosol and membrane with AA alone caused clear translocation of p 47 phox and p 67 phox to the membrane , but only slight translocation of rac p21s . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| In resting cells , Rac and an associated guanine nucleotide regulatory factor , GDP dissociation inhibitor ( GDI ) , were located only in the cytosol , along with other known oxidase factors , p 47 phox and p 67 phox . ^^^ After activation of neutrophils with phorbol 12 myristate 13 acetate or formyl methionyl leucyl phenylalanine , Rac was translocated from the cytosol to the plasma membrane , and this translocation corresponded temporally with the translocation of p 47 phox and p 67 phox and with the generation of superoxide . ^^^ Determination of the quantities of cytosolic factors associated with the plasma membrane indicated that Rac , p 47 phox , and p 67 phox are translocated to the plasma membrane simultaneously in equimolar amounts , but that the membrane associated cytochrome b was present at 3 4 fold molar excess . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| However , HGF PI3K Akt forkhead signaling was insensitive to diphenyleneiodonium and AS p47phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of this multimolecular enzyme minimally requires assembly at the membrane with flavocytochrome b 558 of cytosolic components p47phox , p67phox , and Rac proteins . ^^^ Here , using the yeast two hybrid system , we provide data demonstrating in vivo interactions between human p47phox , p67phox , and Rac proteins . ^^^ Rac proteins interact with p67phox in a GTP dependent manner , but do not interact with p47phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Generation of the microbicidal oxidative burst in human neutrophils requires participation of four proteins , a membrane bound flavocytochrome beta 558 , two soluble proteins termed p 47 phox and p 67 phox , and the Ras related GTPase Rac . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of the superoxide generating NADPH oxidase in phagocytic cells requires the assembly of a membrane bound flavocytochrome b and cytosolic factors p47phox and p67phox under the control of the GTP binding protein , Rac . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The following facts have become evident : ( 1 ) activation of rac and subsequent interaction with p 67 phox is crucial for the interaction of p 67 phox with cytochrome b 558 , and probably with gp 91 phox ; ( 2 ) p 47 phox interacts with p 22 phox , and phosphorylation of 379Ser of p 47 phox is obligatory for this event ; ( 3 ) p 47 phox and p 67 phox regulate each other ' s translocation in a positive sense ( see also reference 71 ) . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| On neutrophil stimulation , the cytosolic components of NADPH oxidase , p67phox and p47phox , as well as the Ras related G protein rac 2 , are translocated from the cytosol to cell membranes where they associate with a flavocytochrome b to form a functional complex . ^^^ In this paper we show that : ( a ) in neutrophils stimulated with formylmethionyl leucylphenylalanine , concanavalin A or phorbol 12 myristate 13 acetate , both rac 1 and rac 2 are translocated from cytosol to the membranes ; ( b ) in neutrophils from a patient with a form of chronic granulomatous disease in which p67phox is absent , rac 2 and p47phox were translocated as in normal neutrophils on stimulation with the above agonists , but rac 1 failed to be translocated from the cytosol to the membranes . ^^^ These results , coupled with those showing that rac 2 is not translocated in activated neutrophils lacking p47phox [ El Benna , Ruedi and Babior ( 1994 ) J . ^^^ Chem . 269 , 6729 6734 ] , may suggest that the assembly of the cytosolic components of NADPH oxidase on the plasma membrane takes place through selective coupling of activated rac 1 and rac 2 with p67phox and p47phox respectively . . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NADPH dependent superoxide generation can be reconstituted in a cell free system using recombinant cytosolic factors ( p 47 phox , p 67 phox , and Rac ) plus flavocytochrome b 558 . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The human NADPH oxidase is a very complex enzyme , in two ways : 1 . it exists of at least 6 components : cytochrome b 558 ( a heterodimer comprised of gp 91 phox and p 22 phox ) , p 47 phox , p 67 phox , p 40 phox , rac and Rap1A , and 2 . there are multiple signal transduction pathways leading to activation of the NADPH oxidase . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of this multimolecular enzyme , minimally requires assembly at the membrane with flavocytochrome b 258 of cytosolic components p47phox , p67phox and Rac proteins . ^^^ Using the yeast two hybrid system , we provide data demonstrating in vivo interactions between human p47phox , p67phox , and Rac proteins . ^^^ Rac proteins interact with p67phox in a GTP dependent manner , but do not interact with p47phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The superoxide generating NADPH oxidase complex of phagocytic cells is a multicomponent system containing a membrane bound flavocytochrome b and a small G protein Rac as well as cytosolic factors p67phox , p47phox and p40phox which translocate to the membrane upon activation . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The cytosolic proteins p47phox and p67phox , each containing two SH 3 domains , are required for activation of the superoxide producing phagocyte NADPH oxidase in a cell free system with human neutrophil membrane and the small GTPase Rac . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The formation of the active oxidase complex at the membrane requires translocation of the Rac GTPase and two specialized cytosolic proteins that harbor SH 3 domains , p67phox and p47phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| When neutrophils are exposed to a variety of particulate or soluble stimuli , the oxidase becomes activated , due to the assembly on the membrane bound flavocytochrome b of three cytosolic factors , p47phox , p67phox and Rac 2 ( or Rac 1 ) . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Active NADPH oxidase can be reconstituted in a cell free system , consisting of phagocyte derived membranes , containing cytochrome b 559 , and the recombinant cytosolic proteins p 47 phox , p 67 phox , and Rac , supplemented with an anionic amphiphile as an activator . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Altogether , the results suggest that , in addition to the phosphorylation of p47phox , the activation of NADPH oxidase requires the activation of a Rac protein through a pathway that diverges at a point upstream of MEK and that is independent of the activation of wortmannin sensitive PI3K . . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| It is a multicomponent enzyme comprising a membrane bound flavocytochrome ( cytochrome b 558 ) and at least four cytosolic components : p47PHOX , p67PHOX , p40PHOX , and Rac , a small GTPase . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| It consists of membranous cytochrome b 558 composed of gp 91 phox and p 22 phox , and four cytosolic components , p 47 phox , p 67 phox , rac p 21 and p 40 phox , which translocate to the membrane upon activation . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| When added prior to the enzyme activation , laurylamine blocked translocation to the membrane of all three cytosolic components ( p 47 phox , p 67 phox and rac ) in a cell free translocation assay . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Upon stimulation , a functional NADPH oxidase is assembled when the cytosolic proteins , Rac , p67phox , p47phox , and possibly p40phox , associate with the gp91phox and p22phox transmembrane proteins . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we hypothesized that Rac 2 translocates along with p47phox and p67phox proteins from the cytosol to the plasma membrane during respiratory burst . ^^^ In subcellular fractions , Rac 2 was found to translocate , along with p47phox and p67phox , from cytosol to plasma membrane associated fractions following phorbol myristate acetate stimulation , while RhoGDI remained within cytosolic fractions . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase , a superoxide producing enzyme in phagocytic cells , consists of membrane associated cytochrome b 558 and cytosolic components ( p 47 phox , p 67 phox , p 40 phox , rac 1 / 2 ) . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| It consists of membranous cytochrom b 558 composed of gp 91 phox and p 22 phox , and four cytosolic components , p 47 phox , p 67 phox , rac p 21 and p 40 phox , which translocate to the membrane upon activation . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of the NADPH oxidase requires translocation of the cytosolic subunits p47phox ( phagocyte oxidase ) , p67phox , and the low molecular weight GT Pase Rac , to the membrane bound flavocytochrome , a heterodimer composed of the heavy chain gp91phox and the light chain p22phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase activity depends on the assembly of the cytosolic activating factors , p 67 phox , p 47 phox , p 40 phox , and Rac with cytochrome b ( 558 ) . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Superoxide generating NADPH oxidase consists of the membrane bound cytochrome b 558 ( gp91phox and p22Phox ) and the cytosolic components ( p67phox , p47phox , p40phox and rac ) . ^^^ RESULTS : Northern blot and Western blot analyses revealed that mRNAs and proteins for gp91phox , p67phox and p47phox were expressed after eosinophilic myelocyte stages , whereas mRNAs and proteins for p40phox and rac 2 were expressed from the promyelocyte stage . ^^^ Consistent with the results of Western blotting , immunocytochemical staining of butyrate induced HL 60 clone 15 cells indicated that gp91phox , p22phox , p67phox and p47phox were detected after eosinophilic myelocyte stages ( eosinophilic myelocytes , eosinophilic metamyelocytes , eosinophilic band cells and eosinophilic segmented cells ) , whereas p40phox and rac 2 were expressed from the promyelocyte stage . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| As with the neutrophil NADPH oxidase , the B lymphocyte NADPH oxidase consists of a membrane bound flavocytochrome b and regulatory factors including Rac and the cytosolic phox protein triad p67phox , p47phox , and p40phox . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| A number of cytosolic proteins are involved in its activation / deactivation : p47phox , p67phox , p40phox and the small GTP binding protein , rac . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| A prenylated p67phox Rac 1 chimera elicits NADPH dependent superoxide production by phagocyte membranes in the absence of an activator and of p47phox : conversion of a pagan NADPH oxidase to monotheism . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of the phagocyte NADPH oxidase complex requires assembly of the cytosolic factors p47PHOX , p67PHOX , p40PHOX , and Rac with the membrane bound cytochrome b 558 . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| In a previous study , the S100A8 / A9 protein , a Ca2+ and arachidonic acid binding protein , abundant in neutrophil cytosol , was found to potentiate the activation of the redox component of the O 2 generating oxidase in neutrophils , namely the membrane bound flavocytochrome b , by the cytosolic phox proteins p67phox , p47phox and Rac ( Doussire J . , Bouzidi F . and Vignais P . ^^^ The S100A8 / A9 heterodimeric protein comigrated with the cytosolic phox proteins , and more particularly with p67phox and Rac 2 , whereas the isolated S100A8 protein displayed a tendancy to bind to p47phox . ^^^ Using a semirecombinant cell free system of oxidase activation consisting of recombinant p67phox , p47phox and Rac 2 , neutrophil membranes and arachidonic acid , we found that the S100A8 / A9 dependent increase in the elicited oxidase activity corresponded to an increase in the turnover of the membrane bound flavocytochrome b , but not to a change of affinity for NADPH or O 2 . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The other components of the respiratory burst oxidase are water soluble proteins of cytosolic origin , namely p67phox , p47phox , p40phox and Rac . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| However , these accumulations of Rac in the membrane , as well as that of p47phox and p67phox , were also regulated by Src tyrosine kinases . ^^^ Inasmuch as Rac GTPases are part of the NADPH oxidase and the respiratory burst is elicited in neutrophils adherent by beta 2 integrins , our results indicate that activation of the NADPH oxidase does not depend on the levels of Rac GTP but instead requires a beta 2 integrin induced targeting of the Rac GTPases as well as p47phox and p67phox to the plasma membrane . . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The cytochrome becomes activated by interacting with the adaptor proteins p47phox and p67phox as well as the small GTPase Rac . ^^^ The protein p51nox as well as p67phox can form a complex with p47phox and with p41nox via the C terminal SH 3 domain and binds to GTP bound Rac via the N terminal domain containing four tetratricopeptide repeat motifs . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The NADPH oxidase complex of phagocytes comprises a membrane associated flavocytochrome b 559 , and 4 cytosolic components : p47phox , p67phox , p40phox , and the small GTPase Rac . ^^^ Activation of the oxidase in vivo is the result of assembly of the cytosolic components with cytochrome b 559 and is mimicked in vitro by a cell free system consisting of membranes , p47phox , p67phox , nonprenylated or prenylated Rac , and an anionic amphiphile as activator ( defined as `` p47phox and amphiphile dependent ' ' or canonical pathway ) . ^^^ We reported that prenylated Rac 1 is capable of activating the NADPH oxidase in vitro in the absence of p47phox and amphiphile ( defined as `` p47phox and amphiphile independent ' ' pathway ) . ^^^ It is proposed that preferential inhibition of oxidase activation , via the p47phox and amphiphile independent pathway , is a reflection of interference by the inhibitors with Rac dependent recruitment of p67phox to the membrane . . ^^^ |
|
| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Because it was recently reported that phosphatidylinositol 3 kinase ( PI3K ) and its downstream effector Akt are implicated in the activation of eNOS and that regulation of VCAM 1 expression is mediated via nuclear factor kappaB ( NF kappaB ) , we hypothesized that celiprolol activates phosphorylation of eNOS through the PI3K Akt signaling pathway ; that celiprolol modulates VCAM 1 expression , which is associated with inhibiting NF kappaB phosphorylation ; and that celiprolol suppresses NAD ( P ) H oxidase p22phox , p47phox , gp91phox , and nox 1 expression in the left ventricle of deoxycorticosterone acetate ( DOCA ) salt hypertensive rats . eNOS and Akt phosphorylation upregulated by celiprolol alone were suppressed by treatment with celiprolol plus wortmannin . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| All vascular cells , including endothelial cells and smooth muscle cells , express components of the leukocyte NADPH oxidase such as p22phox , p47phox , and Rac . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The oxidase , dormant in resting cells , becomes activated to produce superoxide , a precursor of microbicidal oxidants , by interacting with the adaptor proteins p47phox and p67phox as well as the small GTPase Rac . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase activation involves the assembly of membrane localized cytochrome b 559 with the cytosolic components p47phox , p67phox , and the small GTPase Rac . ^^^ We reported that a chimeric construct , consisting of residues 1 212 of p67phox and full length Rac 1 , activates the oxidase in vitro in an amphiphile dependent manner , and when prenylated , in the absence of amphiphile and p47phox . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The superoxide producing phagocyte NADPH oxidase consists of a membrane bound flavocytochrome b 558 complex , and cytosolic factors p47phox , p67phox and the small GTPase Rac , which translocate to the membrane to assemble the active complex following cell activation . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Immunological assays have demonstrated the presence of p67phox and p47phox in lens tissue , while PCR and RT PCR reactions amplified DNA products corresponding to the p67phox , p40phox , p22phox , gp91phox , and Rac 1 components of the NADPH oxidase complex from human and mouse lens cDNA libraries . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| During activation of the cell cytosolic phox proteins ( p47phox , p67phox , p40phox , and Rac 2 ) translocate to the plasma membrane and specific granules fuse with the plasma membrane increasing the amount of flavocytochrome b ( 558 ) . ^^^ Experiments with recombinant p47phox , p67phox , and dilute cytosol or fractionated cytosol as a source of Rac demonstrated that specific granules have requirements identical to specific granules for oxidase activity . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of phosphoinositol 3 kinase and Akt appears to mediate this NADPH oxidase activation through p47phox phosphorylation and Rac 1 activation . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| For example , novel domains / motifs have been identified in p 47 phox ( PX and super SH 3 domains ) and p 67 phox ( tetratricopeptide repeat motifs ) . 10 ray crystallography and NMR spectroscopy have provided detailed structural data on these domains and how p 47 phox and p 67 phox interact with p 22 phox and activated Rac , respectively . ^^^ Site directed mutagenesis and knockout experiments have identified the critical phosphorylation sites in p 47 phox , revealed an `` activation domain ' ' in p 67 phox , and demonstrated that a specific pathway exists for activating Rac to participate in oxidase assembly / activation . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Upon cell stimulation , the redox component , membrane bound flavocytochrome b 558 , is activated when the cytosolic factors ( p47phox , p67phox and p40phox , as well as the small GTPase Rac ) associate with it after translocating to the membrane . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The oxidase activation requires the small GTPase Rac and the SH 3 domain containing proteins p47phox and p67phox ; they normally exist in the cytoplasm and translocate upon cell stimulation to the membrane . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Activation of the phagocyte NADPH oxidase is the consequence of the assembly of membranal cytochrome b 559 with the cytosolic components p47phox , p67phox , and the GTPase Rac and is mimicked by a cell free system comprising these components and an activator . ^^^ We designed a variant of this system , consisting of membranes , p67phox ) prenylated Rac 1 GDP , and the Rac specific guanine nucleotide exchange factor ( GEF ) Trio , in which oxidase activation is induced in the absence of an activator and p47phox . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Cytoskeletal disruption with cytochalasin prevented p47phox : actin interaction and attenuated ROS formation and p38MAP kinase and Akt phosphorylation by Ang 2 . ^^^ CONCLUSIONS : The p47phox : actin interaction , through cortactin , plays an important role in Ang 2 mediated site directed assembly of functionally active NAD ( P ) H oxidase , ROS generation , and activation of redox sensitive p38MAP kinase and Akt , but not ERK1 / 2 . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| In vitro analysis of oxidase activation as well as protein protein interaction studies revealed that S100A8 is the privileged interaction partner for the NADPH oxidase complex since it bound to p67phox and Rac , whereas S100A9 did interact with neither p67phox nor p47phox . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| This reaction is activated by association of cytosolic factors , p47phox and p67phox , and a small G protein Rac with the membranous flavocytochrome b 558 . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The main factors controlling O 2 production by NOX 2 are the cytosolic proteins p67phox and p47phox , and Rac , a small GTP binding protein . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The active oxidase consists of two membrane proteins , gp91phox and p22phox , and four cytosolic proteins , p40phox , p47phox , p67phox , and Rac . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| The activity of gp91phox , the catalytic subunit of the superoxide generating respiratory burst oxidase , is stimulated by the regulatory subunits p47phox , p67phox and the small GTPase Rac . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting of G . mellonella hemocytes with antibodies raised against human neutrophil phox proteins revealed the presence of proteins homologous to gp91phox , p67phox , p47phox , and the GTP binding protein rac 2 . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| Active forms of NAD ( P ) H oxidases are associated with plasma membrane and consist of at least six components , namely : NOX , p22phox peptides and p47phox , p67phox , p40phox and Rac . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| In isolated LR fractions from Fas stimulated endothelial cells , gp91phox , p47phox ( a crucial cytosolic regulatory subunit of NADPH oxidase ) , and Rac GTPase were markedly increased and blocked by nystatin , a compound that disrupts LRs . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase activation is initiated by the translocation to the membrane of the cytosolic components p47phox , p67phox , and the GTPase Rac . ^^^ The prototype chimera p67phox ( 1 212 ) Rac 1 ( 1 192 ) is a potent activator in a cell free system , also containing membrane p47phox and an anionic amphiphile . ^^^ Chimeras behave like bona fide GTPases and can be prenylated , and prenylated ( p67phox Rac 1 ) chimeras activate the oxidase in the absence of p47phox and amphiphile . ^^^ |
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| Interacting proteins: P14598 and P31749 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Mamushi aqueous extract markedly suppressed fMLP induced superoxide generation in a concentration dependent manner . fMLP induced translocation of cytosolic compounds p47phox , p67phox and Rac to the cell membrane was suppressed in parallel to the suppression of superoxide generation . ^^^ |
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