| Here we show binding of PKB to periplakin , a member of the plakin family of cytolinker proteins . ^^^ Interaction between PKB and periplakin was mapped to part of the pleckstrin homology ( PH ) domain of PKB , which is probably not involved in lipid binding , and indeed binding to periplakin did not affect PKB activation . ^^^ We therefore investigated the possibility that periplakin may act as a scaffold or localization signal for PKB . ^^^ Overexpression of the C terminal part of periplakin , encompassing the PKB binding region , results in predominant intermediate filament localization and little nuclear staining . ^^^ These results suggest a possible role for periplakin as a localization signal in PKB mediated signalling . . ^^^ |