| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| Akt protein kinase enhances human telomerase activity through phosphorylation of telomerase reverse transcriptase subunit . ^^^ We noticed two putative Akt kinase phosphorylation sites ( 220GARRRGGSAS229 ) and ( 817AVRIRGKSYV826 ) in human telomerase reverse transcriptase ( hTERT ) subunit . ^^^ In addition , we observed the enhancement of telomerase activity with the pretreatment of Akt kinase in vitro . ^^^ Thus , these observations suggest that Akt kinase enhances human telomerase activity through phosphorylation of hTERT subunit as one of its substrate proteins . . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| We also demonstrate that increased telomerase activity triggered by these cytokines is mediated by phosphatidylinositol 3 ' kinase ( PI3k ) / Akt / nuclear factor kappaB ( NFkappaB ) signaling . ^^^ We confirm involvement of PI3k / Akt / NFkappaB signaling because the PI3k inhibitors wortmannin and LY 294002 or the inhibitor of NFkappaB ( IkappaB ) kinase inhibitor PS 1145 block constitutive and cytokine induced up regulation of telomerase activity . ^^^ Therefore , our results demonstrate that telomerase activity is related not only to transcriptional regulation of hTERT by NFkappaB but also to posttranscriptional regulation because of phosphorylation of hTERT by Akt kinase . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| Expression of human telomerase reverse transcriptase ( hTERT ) catalytic subunit , which modulates telomerase activity , is regulated at both the transcriptional level and via phosphorylation by Akt kinase . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| The catalytic subunit , telomerase reverse transcriptase ( TERT ) , is regulated by interaction with the 90 kDa heat shock protein ( HSP 90 ) and by Akt dependent phosphorylation . ^^^ Treatment of cells with novobiocin , which blocks C terminal interaction of HSP 90 , disrupted HSP 90 binding to Akt , induced Akt dephosphorylation and significantly reduced telomerase activity . ^^^ Likewise , the induction of Akt dephosphorylation by protein phosphatase 2A ( PP2A ) reduced telomerase activity . ^^^ Akt ( T308D / S473D ) prevented novobiocin induced reduction of telomerase activity and the stimulation of apoptosis . ^^^ These data suggest that the association between HSP 90 , Akt and TERT in concert with the phosphorylation of TERT is necessary for maintaining telomerase activity and inhibition of apoptosis . . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| In summary , IGF 1 clearly stimulates telomerase activity in prostate cancer cells through a dual mode of action , including early rapid effects probably involving phosphorylation of hTERT by Akt and later up regulation of hTERT expression . . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| Incubation of a rice cell extract with Akt or with protein phosphatase 2A potentiated or inhibited telomerase activity , respectively , whereas Akt did not affect the activity in Arabidopsis cell extract . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| IL 2 increases human telomerase reverse transcriptase activity transcriptionally and posttranslationally through phosphatidylinositol 3 ' kinase / Akt , heat shock protein 90 , and mammalian target of rapamycin in transformed NK cells . ^^^ Immunoprecipitates of Akt , Hsp 90 , mTOR , and S6K from IL 2 stimulated NK 92 cells contained telomerase activity . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| While mutations of all putative Akt phosphorylation sites in telomerase reverse transcriptase ( TERT ) has no effect on telomerase activity , inhibition of MAP kinase signalling by PD 98059 or U 0126 abolishes NGF induced telomerase down regulation in a concentration dependent manner . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| Raloxifene also induced the phosphorylation of Akt , and pretreatment with a phosphatidylinositol 3 kinase inhibitor , LY 294002 , significantly attenuated the raloxifene induced telomerase activity . ^^^ These results show that raloxifene induced the up regulation of telomerase activity not only by the transcriptional regulation of hTERT but also by post translational regulation of the phosphorylation of Akt and hTERT and the association of hTERT with NFkappaB in HUVECs . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| Moreover , both E 2 and raloxifene induced both the phosphorylation of TERT at a putative Akt phosphorylation site and the association of nuclear factor kappaB with TERT . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| Because the expression of TERT is regulated by the PI 3 K / Akt pathway , we examined the effect of 17beta estradiol on Akt activity in EPCs . ^^^ |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O14746 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
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