Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.1183305
CD 40 binds to a ligand ( CD 40 L ) which is an approximately 35 kDa glycoprotein of 261 aa , a member of the tumor necrosis factor superfamily . 1.1183305^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.62678146
As the interaction between CD 40 ( expressed on B cells ) and its ligand CD 40 L ( expressed on activated T cells ) triggers a key signal required for isotype switching , we studied the expression and function of these two components in normal fetuses , newborns , and infants , compared with adults . 0.62678146^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.66458965
Interactions between CD 40 L and CD 40 induce B cell immunoglobulin production as well as monocyte activation and dendritic cell differentiation . 0.66458965^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.6461686
IgE synthesis by purified human B cells is induced by two signals : a class switching factor , most commonly interleukin ( IL ) 4 , and the engagement of CD 40 , which is activated through its interaction with CD 40 ligand ( CD40L ) expressed on activated T cells . 0.6461686^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.50590377
The production of IL 12 and prostaglandin E 2 is induced by the interaction between CD 40 and CD 40 ligand expressed by Th cells , and by biologically active agents such as micro organisms or their products . 0.50590377^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.56973169
In light of the important role of interleukin 10 ( IL 10 ) and the interaction between CD 40 and CD 40 ligand in the normal regulation of B lymphocyte function and Ig production , we examined these parameters in 24 HIV infected patients . 0.56973169^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.62654583
The interaction between CD 40 ligand ( CD40L ) and its counter receptor CD 40 is critically important in T and B cell costimulation and generation of the humoral immune response . 0.62654583^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.63425499
Some B CLL cases ( eight out of 14 ) are unable to compete for the T cell or CD 40 ligand mediated signal , even though they can functionally interact with CD 40 ligand and thereby get activated themselves . 0.63425499^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.85235456
We determined the role of interactions between CD 40 and CD 40 ligand ( CD40L ) in these infiltrating lymphocytes on B cell differentiation and expression of Bcl 2 family proteins . 0.85235456^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.61134057
Interactions between CD 40 expressed on macrophages and CD 40 ligand expressed on T lymphocytes can be an important signal for optimal macrophage activation . 0.61134057^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.53349945
The interaction of CD 40 ligand ( CD40L ) expressed by activated T cells with CD 40 on macrophages has been shown to be a potent stimulus for the production of IL 12 , an obligate signal for generation of Th 1 cytokine responses . 0.53349945^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.53929028
Besides the well known role of interaction between CD 40 ligand and CD 40 , these pathological conditions definitively demonstrate the requirement of CD 40 mediated NF kappaB activation and the essential role of a newly described molecule , activation induced cytidine deaminase ( AID ) , in B cell terminal differentiation . . 0.53929028^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.87420559
The interaction between CD 40 and its cognate ligand , CD 40 ligand , is a primary regulator of the peripheral immune response , including modulation of T lymphocyte activation , B lymphocyte differentiation and antibody secretion , and innate immune cell activation , maturation , and survival . 0.87420559^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.53901081
In vivo priming of CD 8 ( + ) T lymphocytes against exogenously processed model Ags requires CD 4 ( + ) T cell help , specifically interactions between CD 40 ligand ( CD40L ) expressed by activated CD 4 ( + ) T cells and CD 40 , which is present on professional APC such as dendritic cells ( DCs ) . 0.53901081^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.50667227
Many cytotoxic CD8+ T cell responses are dependent on the interactions between CD 40 ligand on the helper CD4+ T cell and CD 40 on the APC . 0.50667227^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.58081605
The delivery of CD 4 help to CD8+ T cell responses requires interactions between CD 40 and CD 40 ligand and is thought to occur through antigen presenting cell ( APC ) activation . 0.58081605^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.65062842
It is proposed that the macrophage expressed CD 40 interacts with CD 40 ligand on T cells to induce IFN gamma , a Th 1 type cytokine that restricts the amastigote growth . 0.65062842^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.8074819
Interactions between CD 40 ligand ( CD40L ) and CD 40 have been shown to have pluripotent functions in inflammation , including the production of cytokines , chemokines , as well as the angiogenesis factor , vascular endothelial growth factor ( VEGF ) , by endothelial cells . 0.8074819^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.77540449
Interactions between CD 40 ligand and CD 40 are involved in the development of humoral and cell mediated immunity , as well as the activation of macrophages , which are the primary host and effector cells for Mycobacterium tuberculosis . 0.77540449^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.55888521
The interaction between CD 40 ligand ( CD40L ) and CD 40 on antigen presenting cells is essential for the initiation of antigen specific T cell responses . 0.55888521^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.55653777
The interaction between the CD 40 ligand ( CD40L ) and CD 40 on antigen presenting cells ( APCs ) is critical in promoting humoral and cellular immune responses . 0.55653777^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.62395302
Recently , we found that the expression of VEGF is markedly induced following interactions between CD 40 ligand ( CD40L ) and CD 40 . 0.62395302^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.52186315
Interaction of CD 40 with recombinant CD 40 ligand induced apoptosis of NB cells through a caspase 8 dependent mechanism . 0.52186315^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.58446792
Interactions between CD 40 and CD 40 ligand ( CD 154 ) are essential in the regulation of both humoral and cellular immune responses . 0.58446792^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.93716315
Normal , bi directional interactions between CD 40 and its natural ligand CD 154 ( CD 40 ligand ) are central to the generation of both T cell dependent , humoral immune responses and cytotoxic T cell responses . 0.93716315^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.77919102
CD 40 ligand interaction with its receptor ( CD 40 ) not only mediates lymphocyte communication , but also associates with chronic inflammation and atherothrombosis . 0.77919102^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.68980645
I . e . , the IgG response to the Em 2 ( G 11 ) antigen takes place independently of alpha beta+CD4+T cells , and in the absence of interactions between CD 40 and CD 40 ligand . 0.68980645^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.78055079
CD 40 ligand ( CD40L ) expressed by activated CD4+ T cells is a family member of membrane bound TNF family ligand and its interaction with CD 40 expressed in APC has been shown to contribute in enhancing immune response . 0.78055079^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.5802927
BACKGROUND : The interaction between CD 40 ligand ( CD40L ) and CD 40 on antigen presenting cells is essential for the initiation of antigen specific T cell responses , whereas CD40L stimulation of CD40+ tumor cells can induce cellular apoptosis . 0.5802927^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.80731335
Expressed in a murine myeloma , TRAP was identified as a ligand for CD 40 by binding to a soluble CD 40 construct . 0.80731335^^^ Cloning of TRAP , a ligand for CD 40 on human T cells . 0.51966786^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.83748955
Interaction of TRAP with CD 40 on B cells is of paramount importance for immunoglobulin class switching and subsequent synthesis of IgG , IgA or IgE in vivo . 0.83748955^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.61706967
The interaction of TRAP / CD40L with CD 40 thus provides a very critical link between the cellular and the humoral part of the immune system . 0.61706967^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.87447655
The resultant failure of TRAP to interact with CD 40 on functionally intact B cells is responsible for the observed immunoglobulin isotype defect in HIGM1 . . 0.87447655^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.5920744
Immunohistologic analysis of ineffective CD 40 CD40 ligand interaction in lymphoid tissues from patients with 10 linked immunodeficiency with hyper IgM . 0.5920744^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.0282137
The loss of interaction between CD 40 and its ligand in 10 linked hyper IgM results in an impairment of T cell function , of B cell differentiation , and of monocyte function , while only B cell differentiation appears to be affected in autosomal recessive hyper IgM . 1.0282137^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.244198
Interactions between CD 40 and its ligand , CD 154 ( CD40L , gp 39 ) , have been shown to play a central role in the regulation of humoral immunity . 1.244198^^^ Protective immunity induced by tumor vaccines requires interaction between CD 40 and its ligand , CD 154 . 0.80129491^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.54062321
Antibodies preventing the interaction between CD 40 and its T cell based ligand CD 154 ( CD40L ) have been shown in rodents to act synergistically with CTLA 4 Ig . 0.54062321^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.1600487
Interaction of CD 40 with its ligand ( CD 154 ) can induce CD 40 bearing APCs to express immune stimulatory accessory molecules that facilitate immune recognition . 1.1600487^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.60123169
Recent reports have helped explain some of these questions by pointing to a role of contact dependent interaction between CD 40 and CD 40 ligand ( CD40L , renamed CD 154 ) as a stimulus for atheroma associated cells . 0.60123169^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.70051862
Ligation of CD 40 on monocytes through its interaction with CD 40 ligand ( CD 154 ) present on activated T helper cells , results in activation of monocyte inflammatory cytokine synthesis and rescue of monocytes from apoptosis induced through serum deprivation . 0.70051862^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.67422264
These results suggest that the generation of allograft immunity is dependent on the interaction of CD 154 with CD 40 but not primarily for the activation of APCs . . 0.67422264^^^ CD 154 ( CD 40 ligand , gp 39 ) interaction with its receptor CD 40 has been shown to be critically important for the generation of cell mediated as well as humoral immunity . 0.52572902^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.64076356
High sequence homology between human and pig CD 40 with conserved binding to human CD 154 . 0.64076356^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.81920736
By means of murine models , the interaction between CD 40 on DC and its ligand CD 154 has been recognized as an important element for conditioning of DC to prime and expand CTL . 0.81920736^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.63667183
CD 40 interaction with its ligand CD 154 ( CD40L ) has been shown to be an obligatory step in the initiation of autoimmune disease in several animal models . 0.63667183^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.70383747
The interaction between CD 40 and CD 40 ligand ( CD 154 ) activates NF kappa B , Jun N terminal kinase , and Janus kinase / signal transducers and activators of transcription pathways and promotes B cell growth , differentiation , and survival as well as IL 12 production in macrophages and dendritic cells . 0.70383747^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.66227318
These findings indicate that the interaction of platelet CD 154 with CD 40 on neighboring cells is temporally limited to prevent an uncontrolled inflammation at the site of thrombus formation . 0.66227318^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.769212
In particular , the potential involvement of : ( 1 ) the CpG pattern recognition receptor , toll like receptor 9 ; ( 2 ) the dendritic cell specific surface adhesion molecule , DC SIGN ; and ( 3 ) the molecular interactions between CD 40 and CD 154 in the evolution of protective cell mediated immunity to DNA vaccines are discussed . 0.769212^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.79832178
Determination of carbohydrate structures N linked to soluble CD 154 and characterization of the interactions of CD 40 with CD 154 expressed in Pichia pastoris and Chinese hamster ovary cells . 0.79832178^^^ We have characterized the interactions between CD 40 and soluble ( s ) CD 154 in which sCD 154 contains different types of carbohydrates . 0.65200035^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.92569899
The interaction of CD 40 expressed by immunocompetent cells with its ligand CD 154 on the surface of T helper cells plays a crucial role in the immune response . 0.92569899^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.89699765
It is generally accepted that the interaction between CD 40 and its ligand ( CD 154 ) plays a decisive role in contact dependent help for T and B cells . 0.89699765^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.70781199
Interactions between CD 40 and CD 154 provide a number of signals that play important roles in regulating the complex and multifactorial interactions between these two major cell types of the adaptive immune response . 0.70781199^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.76302624
The interaction between CD 40 and its ligand ( CD 154 ) is crucial for IL 12 production and effective humoral immunity such as IgE production . 0.76302624^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.56406326
These results suggest that the interaction of CD 154 expressed by activated CD 4 T cells with CD 40 expressed by B cells is the primary pathway necessary to achieve B cell activation and differentiation and that CD 154 expression on B cells does not noticeably facilitate B cell activation and differentiation . . 0.56406326^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.64857595
Ligation of CD 40 expressed on activated keratinocytes with CD 154 expressed on activated T cells is thought to be involved in the pathogenesis of psoriasis . 0.64857595^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.62136413
Interactions between CD 40 on APC and CD 154 ( CD40L ) expressed by activated CD 4 ( + ) T cells are crucially involved in formation and function of germinal centers ( GC ) , but mechanistic insight into these interactions remains limited . 0.62136413^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.96715657
The interaction between CD 40 and its ligand , CD 154 , has been shown to play a role in the onset and maintenance of inflammatory disease . 0.96715657^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.9841153
In conjunction with previously published data on the clinical benefit of anti CD 40 treatment in the marmoset EAE model , the current findings support antibody mediated blockade of CD 40 interaction with its ligand CD 154 as a potential treatment of MS . . 0.9841153^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.63822236
The treatment with a soluble CD 40 muIg fusion protein ( CD 40 fp ) that prevents the binding of CD 154 with cell surface CD 40 , as well as the transfection with a vector for soluble CD 40 ( KS sCD 40 ) , decreased the anti apoptotic effect of Tat . 0.63822236^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.6105431
The interactions between CD 40 and CD40L ( CD 154 ) are critical for effective humoral immune response . 0.6105431^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.55840692
Antigen induced programmed death does not involve cellular interactions mediated by CD 40 ligand ( CD40L ) or Fas ; disruption of GCs by antibody specific for CD40L was not driven by apoptosis and C57BL / 6 . lpr mice , though unable to express the Fas death trigger , remained fully susceptible to soluble antigen . 0.55840692^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.66524884
CD 40 interacts with CD40L , a 30 33 kD activation induced CD4+ T cell surface molecule . 0.66524884^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.1572129
Interactions between the B cell surface antigen CD 40 and its ligand ( CD40L ) expressed on activated T cells play a critical role in isotype switching . 1.1572129^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.71194328
The interaction of CD40L with CD 40 on B cells results in B cell activation , immunoglobulin ( Ig ) secretion and Ig class switching . 0.71194328^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.57258912
The induction of a T cell dependent Ab response is mediated by the interaction of the T cell activation Ag , CD 40 ligand ( CD40L ) , with CD 40 . 0.57258912^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.53734866
The interaction of CD 40 ligand ( CD40L ) on activated T cells with CD 40 on B cells , monocytes and dendritic cells is essential for humoral immunity and for up regulation of antigen presenting cell ( APC ) functions , as a result of signaling through CD 40 . 0.53734866^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.77262217
Interaction of CD40L with its receptor CD 40 induces proliferation of and isotype switching in B lymphocytes . 0.77262217^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.57658469
Interactions between CD40L on activated T cells and CD 40 on APC may serve to sustain , enhance or prolong the presentation of B 7 1 or B 7 2 on the APC , and thus to prevent anergy induction , or ineffective or abortive T cell stimulation . 0.57658469^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.78113951
The B cell surface molecule CD 40 may be activated either by its ligand CD40L or by anti CD 40 antibodies . 0.78113951^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.6648232
Interactions between CD 40 on antigen presenting cells and its ligand ( CD40L ) on T cells has been implicated in T cell mediated immune responses . 0.6648232^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.90626256
The interaction of CD 40 with its ligand ( CD40L ) , which is expressed on activated T cells , plays a pivotal role in the generation of the T dependent ( TD ) immune response , and FasL bearing T cells , which have been shown to be predominantly of either the TH 0 or TH 1 type , have the potential to induce the apoptotic death of Fas expressing B cells . 0.90626256^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.90352893
BACKGROUND : The interaction between CD 40 and its ligand CD40L is essential for the development and maintenance of vigorous immunity . 0.90352893^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.5597596
Interaction between CD 40 and the CD 40 ligand ( CD40L ) is required for mouse mammary tumor virus ( MMTV ) propagation . 0.5597596^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.51802973
The interaction between CD 40 and CD40L plays important roles in immune responses . 0.51802973^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.72307237
Interactions between CD 40 and CD40L play a central role in the regulation of both humoral and cellular immunity . 0.72307237^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.71498997
The second signal is delivered by interaction of the B cell surface antigen CD 40 with its ligand ( CD40L ) expressed on activated T cells . 0.71498997^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.50367184
It has been reported that ligation of CD 40 with CD 40 ligand ( CD40L ) results in microglial activation as evidenced by p44 / 42 mitogen activated protein kinase ( MAPK ) dependent tumor necrosis factor alpha ( TNF alpha ) production . 0.50367184^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.54179003
Interaction between CD 40 and the CD 40 ligand ( CD40L ) is critical for the survival and proliferation of B cells during immunopoiesis . 0.54179003^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.5596237
CD 40 on epidermal tumor cells interacts with lymphocytes expressing ligand for CD 40 ( CD40L ) or monoclonal antibodies against CD 40 with a significant decrease in proliferation . 0.5596237^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.76713448
The interactions between CD40L and CD 40 within treated tumors were critical because tumor suppression was abrogated by coadministration to the tumors of neutralizing monoclonal antibody against CD40L along with the DCs . 0.76713448^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.85926179
The possible interaction of CD 40 with CD40L may be particularly important during events such as menstruation and cervical ripening , where up regulation of the proinflammatory molecules IL 6 and IL 8 is viewed as critical for these processes . 0.85926179^^^ In fibroblasts from several tissues , the engagement of CD 40 with its ligand ( CD40L ) resulted in the secretion of proinflammatory molecules such as interleukin 6 ( IL 6 ) and IL 8 . 0.51559583^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.71250514
We studied strategies for the reliable generation of such a tumor vaccine by functional maturation of DCs via interaction of CD 40 with its ligand ( CD40L , CD 154 ) . 0.71250514^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.2975542
Here , we examined the possible effect of a major pathway of immune activation , CD 40 interaction with its ligand ( CD40L ) , on the susceptibility of monocyte derived macrophages ( MDMs ) to various HIV 1 strains . 1.2975542^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.55132985
CD40L interaction with CD 40 is required for normal cellular immune responses such as T cell mediated activation of monocytes / macrophages , proinflammatory cytokine production , and leukocyte extravasation . 0.55132985^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.58175535
BACKGROUND : Interaction of CD40L with its receptor CD 40 is critically involved in inflammatory cell activation in atherogenesis . 0.58175535^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.55786303
BACKGROUND : Interaction between CD 40 and CD 40 ligand ( CD40L ) is involved in both cognate and innate immune responses . 0.55786303^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.65764139
BACKGROUND : The interaction between CD 40 and CD 40 ligand ( CD40L ) provides a signal that contributes to the initiation of cellular immune responses . 0.65764139^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.81235781
Analysis of the signal transduction pathways triggered by activated helper T cell in resting B cells . gp 39 is expressed on anti CD 3 activated Th , and binds CD 40 on the B cell , driving B cell cycle entry . 0.81235781^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.0236985
Interaction between CD 40 and its ligand gp 39 in the development of murine lupus nephritis . 1.0236985^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.1451818
The interactions between CD 40 and its ligand , gp 39 , are central to the development and maintenance of immunity . 1.1451818^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :1.3617215
Interactions between CD 40 and its ligand , gp 39 , and the CD28 / CTLA 4 and B 7 family members play a decisive role in regulating the progression of cognate interactions . 1.3617215^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.76659743
Studies using an antibody that neutralizes gp 39 function in vivo have demonstrated that interactions between CD 40 and gp 39 are essential for primary and secondary thymus dependent humoral immune responses , as well as for the generation of memory B cells . 0.76659743^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.59987417
Herein we show that blockage of glycoprotein ( gp ) 39 and CD 40 interaction with gp 39 monoclonal antibody ( mAb ) results in the failure to induce both autoimmune oophoritis and autoantibody production . 0.59987417^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.91518609
The main objective of our study was to determine the role of interactions between gp 39 and CD 40 in `` an established ' ' human Graves ' disease ( GD ) . 0.91518609^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 154 antigen induced by PKA activation of newborn cells was functional , since upon binding to CD 40 on B lymphocytes in the presence of interleukin 4 ( IL 4 ) , it promoted immunoglobulin heavy class switching to IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using this assay , activated CD 4 peripheral blood T lymphocytes from two patients suspected of having HIM syndrome , and from their mothers , were subjected to immunofluorescent flow cytometric analysis for the expression of CD 40 ligand ( CD 154 antigen ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , CD4+ T cell clones from a hyper IgM patient expressing a truncated CD 40 ligand ( CD 40 L ) failed to induce BCP proliferation . ^^^ Finally , a combination of cytokines ( IL 2 , IL 3 , IL 7 , and IL 10 ) enhanced the observed T cell dependent BCP proliferation , but could not substitute for the deficient CD 40 L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The NH 2 terminal amino acid sequence of purified T BAM was determined and found to be highly homologous to the predicted NH 2 terminal sequence of a T cell ligand to the B cell CD 40 molecule ( CD 40 L ) . ^^^ From a D1 . 1 cDNA library , a clone was isolated that encodes CD 40 L by sequence and drives expression of T BAM protein on transfected cells , demonstrating that the T BAM and CD 40 L genes and proteins are identical . ^^^ Taken together , these data unify the biology and structure of T BAM and CD 40 L and this synthesis has implications for understanding the T cell regulation of the humoral immune response . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We isolated and characterized the human genomic clone for CD 40 L to obtain information about the transcriptional regulatory regions of the gene and to develop a molecular diagnostic method for 10 HIM patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A major role in the regulation of murine and human B cell survival and differentiation has been attributed to CD40 / CD40 ligand ( CD 40 L ) interactions , but recent in vitro and in vivo data implicate that other receptor ligand pairs might also be involved . ^^^ In addition , activated T cells also prevented BCR triggered apoptosis , and this effect was inhibited completely by a combination of blocking Abs against CD 40 L and TNF alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It was found that B cells stimulated with CD 40 L alone were more sensitive to Fas mediated apoptosis than were cells stimulated with CD40L plus anti IgM or IL 4 , and in particular , the combination of the two . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The proportion of T lymphocytes , mainly CD 4 positive , co expressing the CD 40 ligand ( CD 40 L ) was significantly greater ( P = 0 . 001 ) in the colostrum of 10 breast feeding mothers than in either autologous or heterologous blood . ^^^ As the T cells of newborn infants fail to express the CD 40 L when stimulated in vitro , the in vivo upregulation on milk T lymphocytes may be one of the mechanisms through which the mother transfers immune protection to the suckling infant . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Synovial and skin fibroblasts cultured over CD 40 Ligand transfected L cells ( L CD 40 L ) demonstrate a CD 40 specific increase of DNA synthesis as measured by tritiated thymidine incorporation . ^^^ Costimulation with L CD 40 L and IFN gamma resulted in maximal proliferation . ^^^ CD 40 L activated fibroblasts showed decreased levels of CD 40 , but only marginal alterations of other cell surface antigens . ^^^ Taken together , the present results indicate that fibroblasts express functional CD 40 and suggest a possible role of CD 40 L expressing cells , such as activated T cells and mast cells , in the development of synovium hyperplasia . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We characterized the distribution of CD 40 and CD 40 ligand ( CD 40 L ) in the adult and developing murine thymus . ^^^ Analysis of mice deficient in CD 40 L expression indicated that CD 40 L / CD40 interactions were not required for development of the medullary compartment . ^^^ Overexpression of CD 40 L targeted to thymocytes altered thymic architecture , as reflected by a dramatic loss of cortical epithelial cells , expansion of the medullary compartment , and extensive infiltration of the capsule with a mixture of CD3+ cells , B cells , and macrophages / dendritic cells . ^^^ Reconstitution of lethally irradiated normal mice with lck CD 40 L bone marrow cells also resulted in loss of cortical epithelium and expansion of the medullary compartment . ^^^ Disruption of the normal pattern of thymic architecture and epithelial differentiation as a consequence of increased intrathymic levels of CD 40 L expression points to a role for CD 40 L / CD40 interactions in the normal pattern of epithelial compartmentalization / differentiation within the thymic environment . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functional expression of CD 40 has recently been described on the surface of HUVEC , and activation of these cells with CD 40 ligand ( CD 40 L ) leads to increased adhesion molecule expression . ^^^ CD 40 L transfected fibroblasts , in contrast to their untransfected counterparts , as well as a soluble recombinant human CD 40 L / murine CD8alpha chimeric molecule were able to importantly increase ( by a mean of fourfold ) the production of leukemia inhibitory factor ( LIF ) by HUVEC . ^^^ CD 40 L displayed an additive effect with IL 4 , IL 1alpha , and TNF alpha on the enhancement of LIF secretion . ^^^ Cyclosporin A did not affect LIF synthesis by resting or CD 40 L activated HUVEC , whereas dexamethasone diminished the basal level of LIF production and abrogated the CD 40 L effect . ^^^ The secretions of two other proinflammatory cytokines , granulocyte macrophage CSF and IL 6 , were also increased in the presence of CD 40 L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In cases with minor upregulation of CD 80 or CD 86 , no further stimulation using CD 40 L activation was observed . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
MIF increased expression of B 7 1 by B cells and CD 40 L by T cells in spleens from Schistosoma mansoni infected mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 L blockade as a novel treatment for chronic ITP refractory to corticosteroids and splenectomy ] . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Treatment with the DC maturation factor trimeric CD 40 ligand ( CD 40 L ) either alone or in combination with fl 3 L neither prevented loss of DC class 2 antigens nor disease relapse . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In corticosteroid dependent asthmatic subjects , PCNA , NFkappaB , and CD 40 L expression was higher than in inhaled corticosteroid treated asthmatic ( P < . 001 ) , untreated asthmatic ( P < . 001 and P < . 04 ) , and control ( P < . 01 ) subjects . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We confirmed reverse signaling via 4 1BB L as immobilized 4 1BB in conjunction with CD 40 L , enhanced IL 12beta mRNA and the secretion of IL 12 p 70 in various APC , including monocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expansion of CD 8 ( + ) T cell numbers was generally higher following priming with CD 40 L than with polyinosinic polycytidylic acid ( poly 1 : C ) matured DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Out of a total of 1176 cDNA ' s 21 genes were differentially regulated in three independent experiments , and generally supported a Th 1 type immune response . 10 genes were validated by real time PCR , and 3 genes ( CD 86 , CD 40 L and CD 132 ) were also analyzed by Flow Cytometry for surface protein expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant human CD 40 ligand stimulates B cell proliferation and immunoglobulin E secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Identification of a source of biologically active CD 40 ligand . ^^^ We have identified the murine thymoma line EL 4 as a source of biologically active CD 40 ligand . ^^^ Fc binding revealed that the surface expressed CD 40 ligand on parental EL 4 and EL40 . 9 cells bound its receptor with a single class of high affinity sites ( Kd = 0 . 5 nM ) . ^^^ Fc , confirming the presence of soluble CD 40 ligand in the preparations . ^^^ The results presented here describe , for the first time , a source of membrane bound and soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The induction of the CD 40 ligand was detectable on the cell surface after 1 h , with maximal expression after 8 h of stimulation . ^^^ The T cells expressing CD 40 ligand were predominantly CD 4 positive , although a proportion of CD 8 positive cells also expressed the protein . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Naive cells primed by cytokines , when activated with anti CD 3 mAb , produce a broad spectrum of cytokines , express CD 40 ligand , but are unable to help B cells for Ig synthesis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Co culture of S 19 cells expressing membrane bound CD 40 ligand ( CD40L ) encoded by recombinant baculovirus with EBV transformed B cell lines induced germline transcription of the epsilon gene in the absence of cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) , shows a similar , although somewhat more moderate , synergy with lipoproteins for induction of proliferation and IgM secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , CV 1 / EBNA cells expressing the human CD 40 ligand also induced the proliferation of the ANBL 6 cell line , an effect that was inhibited by the anti IL 6 mAb . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of B 7 with CD28 / CTLA 4 on T cells results in CD 40 ligand expression , interleukin 4 secretion and efficient help for antibody production by B cells . ^^^ This helper activity is , at least in part , mediated by the interaction between the CD 40 ligand on the T cells and CD 40 on the B cells . ^^^ We also demonstrate that more than one signal to the T cell is required for the induction of the CD 40 ligand , one being delivered through the TcR / CD3 complex and the second by ligation of CD 28 with the B 7 molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Polyclonal activation of immature B cells by preactivated T cells : the role of IL 4 and CD 40 ligand . ^^^ We therefore generated mouse erythroleukemia ( MEL ) transfectants which express CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and its role in 10 linked hyper IgM syndrome . ^^^ CD 40 ligand ( CD40L ) on activated T cells binding to CD 40 on B cells is of critical importance for Ig heavy chain switching and rescue of B cells from apoptosis after somatic mutation in the germinal centre . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The recent discovery that defects in the gene encoding the CD 40 ligand are the underlying cause of the 10 linked hyper IgM syndrome highlights the role of CD 40 and its ligand in Ig isotype switching . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the present study , we examined the participation of CD 40 ligand ( L ) CD 40 interaction in T cell dependent B cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand expression is defective in a subset of patients with common variable immunodeficiency . ^^^ Here we report that CD 40 ligand ( gp 39 ) mRNA expression by activated lymphocytes from CVI patients ( n = 31 ) as a group was significantly depressed ( P < 0 . 0001 ) compared with normal controls ( n = 32 ) . gp 39 mRNA expression by activated lymphocytes from 13 CVI patients fell below the normal control range . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CsA also inhibited CD 40 ligand expression and IL 2 , IL 4 , IL 5 , IFN gamma , and GM CSF production by activated CD4+ T cell clones , whereas MPA and BQ were ineffective , indicating that these compounds do not inhibit early events in T cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 is a membrane differentiation antigen constitutively expressed on B cells that induces B cell growth and Ig synthesis after ligation with anti CD 40 mAb or with the recently identified CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand is involved in delivering the T cell induced contact signals . ^^^ Regulation of synthesis of the CD 40 ligand by activated helper T cells contributes to the maintenance of specificity of the antibody response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The cloning of a ligand for CD 40 , and the generation of reagents which can block the interaction of this ligand with its receptor , have demonstrated that the major component of the contact dependent signal leading to B cell activation is CD 40 ligand . ^^^ Studies of individuals lacking functional CD 40 ligand have indicated that signaling through CD 40 is essential for immunoglobulin ( Ig ) heavy chain switching and the production of all isotypes other than IgM . ^^^ In addition to its activities on B cells , CD 40 ligand is stimulatory for cells of monocyte and T lineages suggesting a pleiotropic role for CD 40 ligand in vivo . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blocking of CD 40 with specific mAb , which have no intrinsic B cell stimulatory properties , or the CD 40 ligand with a soluble CD 40 human IgM fusion protein , resulted in dose dependent , but only partial , inhibition of T cell dependent immunoglobulin production with all modes of T cell activation tested . ^^^ A clear correlation was found between the induction of CD 40 ligand expression on the T cells by the different modes of co stimulation and subsequent immunoglobulin production by the B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Similar CD 40 ligand expression on EL 4 thymoma cell lines with widely different helper activity for B lymphocytes . ^^^ In this study we found that CD 40 ligand ( CD40L ) expression was constitutive and very similar on EL 4 B 5 and parental EL 4 cells according to Northern blot and flow cytometry . ^^^ These findings imply that , although CD 40 ligand is necessary for B cell activation by EL 4B5 cells , additional molecule ( s ) must be responsible for the increased helper activity of the EL 4 B 5 clone . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It has been reported that activated Th cells express CD 40 ligand , and the interaction of the CD 40 ligand and CD 40 on B cells results in B cell cycle entry . ^^^ In this report , mechanisms of B cell activation induced by CD 40 CD40 ligand interaction were studied by using an activated Th cell membrane as a source of CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand acts as a costimulatory signal for neonatal thymic gamma delta T cells . ^^^ We have analyzed the ability of the CD 40 ligand ( CD40L ) to activate neonatal thymic gamma delta T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cells regulate expression of CD 40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD 40 . ^^^ The expression of CD 40 ligand ( CD40L ) on activated T cells ( CD4+ T cell clone MT 9 ) is diminished when the T cells are cultured in the presence of B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A recombinant soluble human CD 40 ligand was also shown to inhibit lymphoma proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate the nature of this defect , B cells from tolerant HEL / anti HEL double transgenic mice were incubated with a membrane preparation from activated T cell clones expressing the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These cells share a number of properties with the major class 2 MHC selected CD 4 population , including the ability to express CD 40 ligand upon activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti CD 28 costimulation induced increases on T cells of CD 40 ligand ( CD40L ) , known to promote B cell proliferation and Ig secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of B cell costimulatory function by recombinant murine CD 40 ligand . ^^^ T cell dependent regulation of B cell growth and differentiation involves an interaction between CD 40 , a B cell surface molecule , and the CD 40 ligand ( CD40L ) which is expressed on activated CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When the polyclonal activators Staphylococcus aureus ( SA ) and soluble CD 40 ligand ( CD40L ) were used to stimulate B cells , rapamycin inhibited both interleukin 2 ( IL 2 ) dependent and independent proliferation , as well as IL 2 dependent differentiation into antibody secreting cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this current study , a mAb against the human CD 40 ligand ( 5c8 ) was used to investigate the impact of CD 40 CD40 ligand interactions in the initial activation of normal human peripheral blood B cells and in subsequent proliferation and differentiation . ^^^ B cells were activated by co culture with anti CD 3 stimulated normal T cells . mAb against CD 40 ligand blocked initial T cell dependent B cell activation , as assessed by [ 3H ] uridine incorporation and IL 2R expression . ^^^ Despite the importance of CD 40 CD40 ligand interactions in T cell B cell collaboration , CD 40 ligand deficient T cell clones were found to induce initial activation of B cells and support Ig production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Localization of the genetic defect in 10 linked immunoglobulin deficiency with normal or elevated IgM ( HIGMX 1 ) to the CD 40 ligand gene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transfectants were stimulated by a soluble recombinant form of the CD 40 ligand gp 39 , and the effect on cell viability determined . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Structure of the murine CD 40 ligand gene . ^^^ The mouse CD 40 ligand ( CD40L ) gene was cloned , sequenced and characterized . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We found that recombinant CD 40 ligand ( CD40L ) induced interleukin 8 ( IL 8 ) secretion and enhanced IL 6 , TNF , and lymphotoxin alpha ( LT alpha / TNF beta ) release from cultured H RS cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , we could demonstrate that failure of immunoglobulin production was not due to a defective CD 40 ligand expression on T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent studies show that most patients with 10 linked hyper IgM syndrome have defects in the gene for CD 40 ligand . ^^^ In these patients , the sequence of CD 40 ligand demonstrated mutations . ^^^ Sequencing of the cDNA for CD 40 ligand from these patients did not show mutations . ^^^ In comparison with B cells from controls or patients with hyper IgM syndrome and mutant CD 40 ligand , B cells from the patients with hyper IgM syndrome and normal CD 40 ligand were defective in their ability to secrete IgE ( P < 0 . 02 ) or express activation markers , CD 25 and CD 23 ( P < 0 . 02 ) in response to stimulation with anti CD 40 . ^^^ These findings indicate that hyper IgM syndrome may result from defects in expression of CD 40 ligand by activated T cells or defects in CD 40 mediated signal transduction in B cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Culturing D Lc on CD 40 ligand ( CD40L ) transfected L cells allowed D Lc survival as 50 + / 15 % of seeded cells were recovered after 4 d while only 5 % survived over control L cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , Fiona Durie and colleagues discuss the co stimulatory role of the interaction between CD 40 on B cells and CD 40 ligand ( CD40L , gp 39 ) on T cells , and review evidence that suggests blocking this interaction may induce T cell tolerance . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Later its counterstructure termed CD 40 ligand ( CD40L ) was discovered to be an inducible type 2 glycoprotein of helper T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T blasts from unstimulated HVS transformed clones did not express CD 40 ligand ( CD40L ) mRNA or CD40L protein , whereas a proportion of them constitutively expressed membrane TNF ( mTNF ) alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When stably overexpressed in L 929 , the chimera retained the ligand binding specificity of CD 40 and the basic signalling properties of Fas since an apoptotic response could be induced in a dose dependent manner upon administration of recombinant , soluble CD 40 ligand trimer or immobilized antiCD 40 antibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Local activation of mast cells or basophils , via their Fc epsilon Receptor type 1 bound IgE , would trigger secretion of various cytokines , IL 4 in particular , and expression of CD 21 and CD 40 ligand , which altogether could replace contact with T cells to deliver the co stimulatory signals for localised IgE production . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , B cells failed to proliferate and undergo isotype switching in vitro in response to soluble CD 40 ligand ( sCD40L ) with interleukin 4 ( IL 4 ) but responded normally to lipopolysaccharide ( LPS ) with IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The human 10 linked immunodeficiency , hyper IgM syndrome , is the result of mutations in the CD 40 ligand gene , and the Fas and Fas ligand genes are mutated in two mouse strains , lpr and gld , that develop autoimmune disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This matched the previously reported kinetics of expression of CD 40 ligand ( CD40L ) on activated peripheral T cells , implicating the CD 40 CD40L interaction as a candidate for the initiator of the IL 1 signaling event . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Multivalent , but not divalent , antigen receptor cross linkers synergize with CD 40 ligand for induction of Ig synthesis and class switching in normal murine B cells . ^^^ Previous work also indicated a critical role , in these responses , for CD 40 mediated signaling mediated by binding of the inducible T cell membrane protein , CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , both this mAb and mAb 89 failed to co operate with a soluble construct of the CD 40 ligand , whereas such co operation was seen with the S2C6 antibody . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A cytotoxic CD40 / p55 tumor necrosis factor receptor hybrid detects CD 40 ligand on herpesvirus saimiri transformed T cells . ^^^ Thus it exerted a marked cytotoxic effect in three different transfected cell lines after activation not only with anti CD 40 antibody but also with CD 40 ligand ( CD40L ) in soluble and membrane bound forms . ^^^ Using hybrid transfected baby hamster kidney cells we demonstrated that herpesvirus saimiri transformed human CD4+ T lymphocytes constitutively express bioactive CD 40 ligand on their surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Structural characteristics of CD 40 ligand that determine biological function . ^^^ CD 40 ligand ( CD40L ) is a 33 kDa type 2 glycoprotein which is transiently expressed on the surface of T cells following activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand CD 40 interaction in Ig isotype switching in mature and immature human B cells . ^^^ The CD 40 ligand ( CD40L ) is a member of the TNF family , and has emerged as a key molecule in the contact mediated signal required for B cell activation and differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction between the CD 40 ligand ( gp 39 ) , expressed by activated T cells , and CD 40 , constitutively expressed by B cells , is critical for an effective antibody response to T cell dependent antigens . ^^^ Patients with 10 linked hyper IgM ( HIM ) syndrome fail to express a functional CD 40 ligand due to a mutation within the gene for gp 39 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD 40 ligand in human disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
An engagement of CD 40 with CD 40 ligand ( CD40L ) expressed on activated T cells is known to provide an essential costimulatory signal to B cells in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Both forms of stimulation led to expression of JunB and JunD mRNA and protein ; however , induction of JunB mediated by anti Ig Ab was protein kinase C ( PKC ) dependent , whereas induction mediated by CD 40 ligand was resistant to PKC depletion . ^^^ Although both stimuli induced c Fos , expression of FosB was stimulus specific at both the mRNA and protein levels , in that this component was induced by anti Ig but not by CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti CD 40 as well as soluble CD 40 ligand caused sustained induction of LT alpha on the membrane of the B cells lasting up to 120 h but induced no detectable secretion of LT alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We previously reported that anti CD 3 activated T cells ( Ta ) can induce expression of CD 80 and CD 54 ( ICAM 1 ) on human B cells through a contact dependent signal delivered to the CD 40 molecule via the CD 40 ligand . ^^^ Here , we demonstrate that another molecule in the CD 40 ligand family , namely tumor necrosis factor alpha ( TNF alpha ) , also plays a role in the Ta mediated induction of CD 80 or CD 54 on human B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upregulation ( by IL 4 ) of CD 40 on B cells which then may be stimulated by gp 39 ( CD 40 ligand ) can constitute an early and important event in the IgE mediated allergic reaction . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand triggered interleukin 6 secretion in multiple myeloma . ^^^ Triggering via CD 40 using NIH3T3 CD 40 ligand transfectant ( CD40LT ) cells increased ( > 30 % ) cell surface CD 80 , CD 18 , CD11a , CD11b , and CD11c expression on MM cell lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We studied the role of the C terminal structural unit of TSST 1 with regard to proliferation , cytokine release ( tumor necrosis factor alpha [ TNF alpha ] , interleukin 6 [ IL 6 ] , and IL 8 ) , mRNA expression for IL 6 , IL 8 , IL 10 , TNF alpha , and CD 40 ligand ( CD40L ) , synthesis of immunoglobulin E ( IgE ) , IgA , IgG , and IgM , CD 23 expression , and soluble CD 23 ( sCD 23 ) release from human peripheral blood mononuclear cells ( PBMC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have analyzed for the presence of somatic mutation in Ig gene rearrangements of the nonpolymorphic human VH 6 gene in the 10 linked HyperIgM syndrome , which is associated with defective CD 40 ligand expression and absence of germinal centers and generation of memory B lymphocytes . ^^^ These results suggest that somatic mutation may be initiated in a CD 40 ligand independent pathway before entry of B cells into germinal centers , but fails to achieve the high mutation frequency observed in the presence of germinal centers . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using the soluble recombinant CD 40 ligand , sgp 39 , we show that the interaction of CD 40 with its ligand can lead to endothelial cell activation , which in turn leads to leukocyte adhesion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Therefore , it was of interest to examine ileal PP follicular ( iPf ) B cell responses to CD 40 ligand , a molecule integral to T cell dependent B cell development . ^^^ A variable level of CD 40 expression was detected on a subpopulation of iPfB cells and J558L cells , expressing a membrane form of mouse CD 40 ligand ( mCD40L ) , interacted specifically with the CD 40 molecule on iPfB cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The discovery in 1992 of a natural counterstructure , the CD 40 ligand ( CD40L ) , which can be rapidly induced on T cells has hastened research and increased interest in this area dramatically . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , the different abilities of CD4+ memory T cell subsets to support B cell differentiation did not relate to variations in the expression of CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD4+CD57+ T cells do not express mRNA for a number of different cytokines or for the CD 40 ligand after activation in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand expressed by human B cells costimulates B cell responses . ^^^ The nucleotide sequence of the coding region of the CD 40 ligand mRNA amplified by RT PCR from activated T cells and B cell lines was identical . ^^^ The CD 40 ligand expressed on human B cells was important functionally because homotypic aggregation of CD 40 ligand expressing B cells was inhibited by the CD40 . ^^^ Finally , B cell lines expressing the CD 40 ligand induced Ig production from resting normal B cells in a CD 40 dependent manner . ^^^ These results indicate that human B cells express a ligand for CD 40 that is identical with that expressed by activated T cells and that the B cell expressed CD 40 ligand plays an important role in facilitating responses of activated B cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When purified human germinal center cells were cultured with interleukin 2 , interleukin 10 , and cells expressing CD 40 ligand , cells with characteristics of memory B cells were generated . ^^^ Removal of CD 40 ligand from the system resulted in terminal differentiation of germinal center B cells into cells with the characteristics of plasma cells . ^^^ These results indicate that CD 40 ligand directs the differentiation of germinal center B cells toward memory B cells rather than toward plasma cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that activated T cells induce the production of IL 12 by monocytes via a mechanism involving the interaction of T cell associated CD 40 ligand with CD 40 on monocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A trimeric form of recombinant murine CD 40 ligand acts on human EC to increase expression of leukocyte adhesion molecules , including E selectin , vascular cell adhesion molecule 1 , and intercellular adhesion molecule 1 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A point mutation in exon 2 of the CD 40 ligand gene causes the simultaneous expression of two defective mRNA species in 10 linked hyperimmunoglobulinemia M . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Studies of interactions between DC and phorbol ester activated T cells expressing the CD 40 ligand revealed a fifth independent adhesion pathway , CD40 / CD40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent studies have identified CD 40 ligand ( CD40L ) as the critical membrane expressed molecule responsible for T cell dependent B cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
While the function of CD 40 on fibroblasts is not yet known , it may facilitate fibroblast proliferation , an event important for tissue repair , and may facilitate inflammation via interaction with T lymphocytes and mast cells , which display the CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunodeficiency with hyperimmunoglobulinemia M in two female patients is not associated with abnormalities of CD 40 or CD 40 ligand expression . ^^^ The immunologic defect in 10 linked immunodeficiency and hyperimmunoglobulinemia M ( HIM ) are related to defective expression of the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Roles of CD 40 ligand and B 7 2 in established germinal centers . ^^^ We have compared the roles of CD40L and B 7 2 in the initiation and maturation of humoral immunity by administering anti CD 40 ligand ( L ) or anti B 7 2 Ab during the early ( days 1 to 3 ) or late ( days 6 10 ) phases of primary responses to thymus dependent ( Td ) and independent ( Ti ) Ags . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
HIV gp 120 inhibits T cell activation by interfering with expression of costimulatory molecules CD 40 ligand and CD 80 ( B 71 ) . ^^^ In this study , we have analyzed the role of the interacting pairs of costimulatory molecules , CD 28 B71 ( CD 80 ) and CD 40 ligand ( CD40L ) CD 40 , to elucidate further the mechanism of HIV gp 120 induced inhibitory effects on T cell functions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand / CD40 deficiency . ^^^ The CD 40 ligand ( CD40L ) is expressed on activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of the CD 40 ligand in T lymphocytes and induction of IgE isotype switching . ^^^ CD 40 ligand ( CD40L ) delivers a contact dependent signal to B cells which , in the presence of interleukin ( IL ) 4 , drives immunoglobulin isotype switching to IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Direct physical interaction involving CD 40 ligand on T cells and CD 40 on B cells is required to propagate MMTV . ^^^ The propagation of mouse mammary tumor virus ( MMTV ) has been analyzed in mice defective for expression of CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , these B cells do not respond normally to a number of stimuli , including BCR cross linking and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand has potent antiviral activity . ^^^ In this paper , we also describe our attempts to analyse the CD 40 ligand mediated antiviral activity by studying the clearance of these viruses in mice that are deficient in important antiviral mechanisms . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Getting to know you : viruses meet CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand positive CD8+ T cell clones allow B cell growth and differentiation . ^^^ A fraction of activated CD8+ T cells expresses CD 40 ligand ( CD40L ) , a molecule that plays a key role in T cell dependent B cell stimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cloning of two members of the TNF superfamily in cattle : CD 40 ligand and tumor necrosis factor alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Survival of mouse pancreatic islet allografts in recipients treated with allogeneic small lymphocytes and antibody to CD 40 ligand . ^^^ Combined treatment with allogeneic small lymphocytes or T depleted small lymphocytes plus a blocking antibody to CD 40 ligand ( CD40L ) permitted indefinite pancreatic islet allograft survival in 37 of 40 recipients that differed from islet donors at major and minor histocompatibility loci . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Infiltrating lymphoid cells expressing CD 27 , the CD 70 receptor , and the CD 40 ligand were present in all cases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is constitutively expressed in a subset of T cell lymphomas and on the microenvironmental reactive T cells of follicular lymphomas and Hodgkin ' s disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Patients with HIGM 1 have a defective CD 40 ligand on their activated T helper cells ; therefore , they can not receive signals for isotype switching when the cells are activated by T cell dependent antigens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of the transformed fibroblast cell line SV 80 with CD 40 ligand ( CD40L ) or anti CD 40 Ab resulted in the production of IL 6 ; this could be increased by IFN gamma pretreatment , which is known to up regulate CD 40 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand expression is developmentally regulated in human thymocytes . ^^^ We have investigated the capacity of human thymocytes to express CD 40 ligand . ^^^ CD 40 ligand was not detectable on resting immature double positive ( CD4+ / CD8+ ) or resting mature single positive ( CD4+ / CD8 or CD 4 / CD8+ ) thymocytes . ^^^ Immature thymocytes failed to express CD 40 ligand on their surface after stimulation with PMA and ionomycin , whereas stimulated CD4+ / CD8 mature thymocytes expressed CD 40 ligand . ^^^ Northern blot analysis revealed that CD 40 ligand mRNA was detectable in activated mature single positive thymocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand independent B cell activation revealed by CD 40 ligand deficient T cell clones : evidence for distinct activation requirements for antibody formation and B cell proliferation . ^^^ We report the capacity of CD 40 ligand ( CD40L ) negative T cell clones to activate human B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Spontaneous apoptosis of dendritic cells is efficiently inhibited by TRAP ( CD 40 ligand ) and TNF alpha , but strongly enhanced by interleukin 10 . ^^^ TNF related activation protein ( TRAP ; CD 40 ligand ) and interleukin 10 ( IL 10 ) on induction or prevention of apoptotic cell death in these cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant CD 30 ligand and CD 40 ligand share common biological activities on Hodgkin and Reed Sternberg cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We found that DCs respond to tumor necrosis factor alpha , CD 40 ligand , IL 1 , and lipopolysaccharide with a coordinate series of changes that include downregulation of macropinocytosis and Fc receptors , disappearance of the class 2 compartment , and upregulation of adhesion and costimulatory molecules . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of human B cell function by recombinant CD 40 ligand and other TNF related ligands . ^^^ To assess the potential of CD 40 ligand ( CD40L ) and the related molecules CD 27 ligand ( CD 27 ) , CD 30 ligand ( CD30L ) , and membrane TNF alpha to stimulate B cell responses , expression of these proteins in the baculovirus system was performed . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant murine CD30L was found to share many functional properties with CD 40 ligand ( CD40L ) in the regulation of murine B cell growth and differentiation in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We tried to determine whether this is due to a B cell defect and whether this can be corrected in vitro by IgG production promoting cytokines CD 40 ligand ( CD40L ) and interleukin 10 ( IL 10 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand , gp 39 , is defective in activated T cells from patients with 10 linked hyper IgM syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A small proportion of CD4+ and CD8+ blood mononuclear T cells activated by anti CD 3 expressed the CD 40 ligand but its detection was best observed following depletion of B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand can replace the normal T cell derived CD 40 ligand signal to B cells in T cell dependent activation . ^^^ These data are evidence that the CD 40 ligand on T cells provides a signal that drives B cell proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The regulation of T cell dependent antibody formation in vitro by CD 40 ligand and IL 2 . ^^^ Our study demonstrates the central role for the murine CD 40 ligand and IL 2 in contact dependent T cell help for Ag specific primary antibody responses in vitro . ^^^ Helper T cell clones were found to express CD 40 ligand after activation with CD 3 mAb . ^^^ Membrane bound recombinant CD 40 ligand expressed on fixed CV1 / EBNA cells had similar B cell activating properties as T cell clones that had been activated and then fixed . ^^^ The induction of polyclonal Ig secretion by the recombinant CD 40 ligand required IL 4 and IL 5 although optimal Ag specific antibody formation required IL 2 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show here that HIV infected T cell clones do not express the CD 40 ligand ( CD40L ) , a molecule critical for non cognate B cell activation , but a small proportion of them do express membrane tumour necrosis factor ( TNF ) alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The mechanism of T cell help has also come into much clearer focus through elucidation of the interaction between CD 40 on B cells and the CD 40 ligand on activated T cells . ^^^ Finally , progress has been made with the recent identification of defects in a cytoplasmic protein tyrosine kinase and in the CD 40 ligand as causes of two B cell immunodeficiencies in man . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 was found to mediate monocyte adhesion to cells expressing recombinant CD 40 ligand . ^^^ CD 40 ligand transfected cells provided a potent costimulus for monocyte TNF alpha and IL 6 production in the presence of GM CSF , IL 3 , or IFN gamma , and enhanced IL 8 production stimulated by GM CSF or IL 3 . ^^^ In addition , CD 40 ligand transfected cells acting in the absence of a costimulus induced monocytes to become tumoricidal against a human melanoma cell target . ^^^ Collectively , these data indicate that CD 40 ligand is pleiotropic with potent biological activity on monocytes . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This review highlights experiments that demonstrate a central role for interactions between T cell B cell activating molecule ( CD 40 ligand ) expressed on T cells and CD 40 on B cells in B cell activation and immunoglobulin isotype switching , both in vitro and in vivo . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Defective expression of the CD 40 ligand in 10 chromosome linked immunoglobulin deficiency with normal or elevated IgM . ^^^ Because interaction between CD 40 and its ligand on activated T cells is critical for T cell driven isotype switching , we examined CD 40 ligand expression . ^^^ In contrast to normal T cells , lymphocytes from the patients expressed no detectable CD 40 ligand on their surface after stimulation with phorbol 12 myristate 13 acetate and ionomycin , although the mRNA of the ligand was expressed normally . ^^^ These results suggest that defective expression of the CD 40 ligand underlies the failure of isotype switching in this disease . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The vav / T and B cells were hyporeactive when stimulated through antigen receptors , but vav / T cells proliferated on exposure to phorbol ester and calcium ionophore , whereas B cells responded normally to bacterial mitogen , lipopolysaccharide or the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is functionally expressed on human eosinophils . ^^^ CD 40 ligand ( CD40L ) , a surface molecule which can be expressed by T cells , mast cells and basophils , has been shown to be involved in the control of B cell proliferation , immunoglobulin class switching as well as in the activation of monocytes and T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant soluble trimeric CD 40 ligand is biologically active . ^^^ CD 40 ligand ( CD40L ) is expressed on the surface of activated CD4+ T cells , basophils , and mast cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 13 induces proliferation and differentiation of human B cells activated by the CD 40 ligand . ^^^ We cloned the human CD 40 ligand ( hCD40L ) from a cDNA library constructed from an activated CD8+T cell clone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Addition of a soluble form of the CD 40 ligand ( sgp 39 ) to the culture system has a similar effect on specific Ab synthesis as anti CD 40 ; addition of the soluble construct , CD 40 Ig , known to inhibit gp39 / CD40 interaction , suppresses in vitro antiphage Ab production by Ag exposed peripheral blood mononuclear cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction by anti CD 40 antibody or soluble CD 40 ligand and cytokines of IgG , IgA and IgE production by B cells from patients with 10 linked hyper IgM syndrome . ^^^ HIGM 1 is caused by a CD 40 ligand gene mutation , leading to defective expression on the membrane of activated T lymphocytes . ^^^ We found that triggering of B cells by an anti CD 40 monoclonal antibody or the soluble CD 40 ligand plus interleukin ( IL ) 4 or IL 10 led to B cell proliferation and / or differentiation towards IgG , IgA and IgE secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is a T cell growth factor . ^^^ CD 40 ligand ( CD40L ) is a 33 kDa type 2 membrane glycoprotein induced on T cells upon activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vivo expression of CD 40 ligand , cytokines , and antibody production delineates sites of cognate T B cell interactions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A subset of CD4+ memory T cells contains preformed CD 40 ligand that is rapidly but transiently expressed on their surface after activation through the T cell receptor complex . ^^^ Preformed CD 40 ligand ( CD40L ) was not detected in any CD4+ CD45RA+ T cells , but > 90 % of all CD4+ T cells from the tonsil can be induced to express large amounts of CD40L on culture with phorbol myristate acetate and the calcium ionophore ionomycin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand ( gp 39 ) and LFA 1 appear to be two major contributors for T cell dependent nitric oxide production . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Characterization of nine novel mutations in the CD 40 ligand gene in patients with 10 linked hyper IgM syndrome of various ancestry . 10 linked immunodeficiency with hyper IgM ( HIGMX 1 ) is a rare disorder caused by defective expression of the CD 40 ligand ( CD40L ) by activated T lymphocytes , resulting in inefficient T B cell cooperation and failure of B cells to undergo immunoglobulin isotype switch . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We find that co cross linking of mouse CR 2 and CR 1 with sIgM rescues both resting B cells and WEHI 231 . 7 cells from apoptosis induced by sIgM ligation in a fashion similar to that found using soluble mouse CD 40 ligand ( mCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In turn , responder T lymphocytes were induced to express B 7 1 / CD80 , B 7 2 / CD86 , CD 40 ligand ( CD40L ) , ICAM 1 , L16 / activated LFA 1 , and HLA DR , reflecting bidirectional signaling between T lymphocytes and B NHL cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we show that G 28 5 induces partial CD 40 responses and functions as a partial antagonist of natural CD 40 ligand , gp 39 , by preventing gp 39 binding . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
By reducing the concentration of anti TcR mAb we obtained low levels of CD 40 ligand ( CD40Llow ) on Th cells , comparable to those expressed by lymph node T cells activated in vitro ( ex vivo T cells ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IgE production is regulated by CD4+ T helper type 2 ( Th 2 ) lymphocytes , producing interleukin 4 ( IL 4 ) and expressing a ligand for the B cell specific CD 40 molecule ( CD 40 ligand [ L ] ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Diminished expression of CD 40 ligand by activated neonatal T cells . ^^^ CD 40 and CD 40 ligand ( gp 39 ) mediate contact dependent T B cell interaction . ^^^ We determined the expression of CD 40 ligand by activated neonatal T cells and the response of neonatal B cells when activated through CD 40 . ^^^ Although expression of CD 40 ligand peaked simultaneously in both activated adult and neonatal cells , neonatal T cells expressed significantly less CD 40 ligand surface protein and mRNA than adult T cells . ^^^ Activated thymocytes also expressed far less CD 40 ligand than adult T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , CD 40 ligand ( CD40L ) and LPS , two PKC independent forms of B cell stimulation , failed to induce phosphorylation of CREB Ser 133 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Exposure of the cells to CD 40 ligand ( CD40L ) or CD 40 monoclonal antibody prevented cell apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These represent the first human disease phenotypes associated with three gene families already recognized to be important in lymphocyte development and signaling : XLA is caused by mutations of a B cell specific intracellular tyrosine kinase ; HIGM by mutations in the tumor necrosis factor related CD 40 ligand , through which T cells deliver helper signals by direct contact with B cell CD 40 ; and SCID by mutations in the gamma chain of the lymphocyte receptor for interleukin 2 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of CD 40 ligand expression and use of recombinant CD 40 ligand for studying B cell growth and differentiation . ^^^ CD 40 ligand can deliver helper T cell derived contact signals to B lymphocytes that drive B cell activation and proliferation . ^^^ Regulation of expression of CD 40 ligand is analogous to that of other helper T cell derived lymphokines . ^^^ A soluble form of CD 40 ligand is capable of delivering proliferative signals to B cells only when cross linked or when IL 4 is added . ^^^ Recombinant CD 40 ligand in membrane vesicles can be used to develop in vitro systems for studying class switching , clonal selection , and affinity maturation in normal B lymphocytes . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T lymphocytes express CD 40 ligand and induce isotype switching in B lymphocytes . ^^^ In an effort to understand the role of gamma / delta T cells in directing B cell responses , we investigated the capacity of human gamma / delta T cells to express CD 40 ligand ( CD40L ) and to drive immunoglobulin ( Ig ) isotype switching in B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mice deficient for the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of NF AT in normal B lymphocytes by anti immunoglobulin or CD 40 ligand in conjunction with IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The Ag independent interaction between the CD 40 ligand , expressed on activated T cells , and its CD 40 receptor , expressed on B cells , enhances B cell proliferation in response to IL 4 stimulation . ^^^ The expression of the CD 40 ligand is induced on CD4+ T cells by stimulation with Ag pulsed APC or mitogens . ^^^ Here , we show that at least some IL 4 producing murine CD8+ T cell clones can be induced to express the CD 40 ligand when stimulated with anti CD 3 mAb . ^^^ Proliferation of small resting B cells cultured with IL 4 in the presence of activated IL 4 producing CD8+ murine T cell clones appeared to be mediated by the expression of the CD 40 ligand on the T cell because an anti CD 40 ligand mAb inhibited this proliferative response . ^^^ A conventional murine CD8+ CTL clone , which did not produce IL 4 or express CD 40 ligand upon activation , did not potentiate proliferation of small resting B cells exposed to IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cells can be viewed in a similar way , with the costimulatory molecule CD 40 ligand and cytokines coming mainly from CD 4 helper T cells determining the fate of the responding B cell . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
C to T mutation causing premature termination of CD 40 ligand at amino acid 221 in a patient affected by hyper IgM syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The molecular defect in 10 linked hyper IgM affects the gene encoding the CD 40 ligand ( CD40L , gp 39 ) on T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cyclosporin A inhibits CD 40 ligand expression in T lymphocytes . ^^^ The mechanisms regulating CD 40 ligand gene expression are largely unknown . ^^^ Optimal expression of CD 40 ligand required activation of protein kinase C and a rise in intracellular calcium concentration . ^^^ CD 40 ligand expression was inhibited by pretreatment of T cells with cyclosporin A . ^^^ Cyclosporin A analogues inhibited CD 40 ligand expression with a potency mirroring the ability of each compound to inhibit calcineurin activity , indicating that calcineurin plays a key role in CD 40 ligand gene expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant CD 40 ligand exerts potent biologic effects on T cells . ^^^ Murine and human CD 40 ligand ( CD40L ) were recently cloned , expressed , and shown to possess potent activity on human and murine B cells , including stimulation of proliferation and Ig secretion in the presence of cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Phenotypic and functional characterization of T BAM ( CD 40 ligand ) + T cell non Hodgkin ' s lymphoma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ineffective expression of CD 40 ligand on cord blood T cells may contribute to poor immunoglobulin production in the newborn . ^^^ It has recently been demonstrated that interaction of the CD 40 molecule on B cells with the CD 40 ligand ( CD40L ) on activated T cells is pivotal for immunoglobulin switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We investigated the capacity of newborn lymphocytes to express the CD 40 ligand and to undergo CD 40 ligand dependent immunoglobulin isotype switching . ^^^ After stimulation by phorbol ester and ionomycin , newborn lymphocytes expressed markedly decreased amounts of CD 40 ligand on their surface compared to normal adult lymphocytes . ^^^ Northern blot analysis of mRNA derived from activated cord blood lymphocytes also revealed markedly decreased amounts of CD 40 ligand mRNA . ^^^ Decreased expression of CD 40 ligand in newborn lymphocytes was associated with a severely decreased ability to undergo T cell dependent immunoglobulin isotype switching . ^^^ These results indicate that decreased expression of CD 40 ligand in newborn lymphocytes may be the underlying cause of deficient immunoglobulin isotype switching in newborns . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) expression and B cell function in agammaglobulinemia with normal or elevated levels of IgM ( HIM ) . ^^^ The 10 linked form ( HIGM 1 ) is a result of mutations in the CD 40 ligand ( CD40L ) gene , but the defect in non 10 linked forms of the disease ( HIM ) has not been determined . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Particularly the identification of new surface membrane molecules such as CD 40 ligand on basophils or c kit on mast cells , and of new triggering agents and modulators of mediator release such as IL 3 , IL 5 , GM CSF and nerve growth factor ( for basophils ) or c kit ligand ( for mast cells ) allows a better understanding of the regulation of these cell types . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand gene of one patient was examined and contained a nonsense mutation at nucleotide 475 . ^^^ CD 40 ligand is a membrane protein which is expressed on activated T cells and induces B cell proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regardless of the priming conditions , primed CD 8 T cells are CD45ROhigh / RA , CD31high and more than 50 % are CD4+ / CD8+ ; activated naive or primed CD 8 T cells do not express CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cell clones from an 10 linked hyper immunoglobulin ( IgM ) patient induce IgE synthesis in vitro despite expression of nonfunctional CD 40 ligand . ^^^ It has been widely reported that a second signal can be provided by the CD 40 ligand ( CD40L ) expressed on the surface of T cells , mast cells , and basophils . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Humoral immune responses in CD 40 ligand deficient mice . ^^^ Individuals with 10 linked hyper IgM syndrome fail to express functional CD 40 ligand ( CD40L ) and , as a consequence , are incapable of mounting protective antibody responses to opportunistic bacterial infections . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human CD 40 ligand : molecular cloning , cellular distribution and regulation of IgE synthesis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The ability of CD4+ cells from HIV+ individuals to express CD 40 ligand after in vitro stimulation is not impaired . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After activation through CD 40 the centrocytes increase their surface Ig and acquire characteristics of memory and processing of antigen held on FDC and its presentation to T cells that can be induced to express CD 40 ligand at the point of cognate interaction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The activity in Th membrane requires the expression of CD 40 ligand by the T cells , and initiation of the B cell response occurs through the ligation of CD 40 on the B cell surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , C alpha 1 and C alpha 2 mature transcript induction is also differentially regulated , with C alpha 2 requiring a T cell signal such as that delivered by the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DSCG did not interfere with CD 40 ligand expression by T cells but clearly targeted the B cells because it inhibited IgE synthesis induced by anti CD 40 and IL 4 in populations of highly purified B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A 3 D model for the CD 40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human B cell proliferation and Ig secretion induced by recombinant CD 40 ligand are modulated by soluble cytokines . ^^^ Recombinant human CD 40 ligand ( hCD40L ) was expressed on the surface of CV1 / EBNA cells and examined for its ability to induce proliferation and Ig secretion from human B cells in the presence or absence of soluble cytokines . hCD40L was directly mitogenic in a dose dependent fashion for purified tonsil B cells with maximal proliferation occurring at days 5 to 7 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant CD 40 ligand stimulation of murine B cell growth and differentiation : cooperative effects of cytokines . ^^^ In this study , we investigate the biological effects of murine recombinant CD 40 ligand . ^^^ The recombinant CD 40 ligand expressed on the CV 1 / EBNA monkey fibroblast cell line directly activated resting B cell to express elevated levels of cell surface class 2 major histocompatibility complex and CD 23 molecules . ^^^ CD 40 ligand also stimulated B cell proliferation , reaching maximal levels on day 2 of culture and declining thereafter . ^^^ By itself , CD 40 ligand had no effect upon immunoglobulin secretion by B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A dimeric soluble form of CD 40 was produced and used to further characterize the regulation of expression of the CD 40 ligand . ^^^ Expression of the CD 40 ligand was rapidly induced after Th lymphocyte activation , and its stability depended upon whether Th cells were activated with soluble or plastic bound stimuli . ^^^ Th cells activated with soluble stimuli rapidly turned over cell surface CD 40 ligand whereas Th cells activated with plastic bound stimuli exhibited more stable CD 40 ligand expression for up to 48 h . ^^^ Removal of activated Th cells from the plastic bound stimulus resulted in a rapid turnover of CD 40 ligand , suggesting that continuous stimulation could maintain CD 40 ligand expression . ^^^ Ligation by soluble CD 40 could also stabilize expression of CD 40 ligand on the Th cell surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dinucleotide repeat polymorphism in the human CD 40 ligand gene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The regulation of the expression of gp 39 , the CD 40 ligand , on normal and cloned CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interaction between the B cell antigen CD 40 and the CD 40 ligand expressed on activated T cells is critical for T cell driven isotype switching . ^^^ We have reported that T lymphocytes from three unrelated male patients with HIGMX 1 failed to express CD 40 ligand on their surface , but the mRNA for CD 40 ligand was of an apparently normal size and level . ^^^ Analysis of CD 40 ligand cDNA from two of the patients revealed deletions that alter the reading frame . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Incubation of these cultured DCs with tumor necrosis factor alpha ( TNF alpha ) or soluble CD 40 ligand ( CD40L ) for 24 h results in an increased surface expression of MHC class 1 and class 2 molecules , B 7 , and ICAM 1 and in the appearance of the CD 44 exon 9 splice variant ( CD 44 v9 ) ; by contrast , Fc gamma RII is markedly and sometimes completely downregulated . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and IL 2 ) for B cell survival and progression to immunoglobulin secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , exposure of GC B cells to IB 4 mAb up regulates the bcl 2 proto oncogene product in a manner similar to that observed with CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These represent the first human disease phenotypes associated with each of three gene families already recognized to be important in lymphocyte development and signaling : XLA is caused by mutations of a B cell specific intracellular tyrosine kinase ; HIGM , by mutations in the TNF related CD 40 ligand , through which T cells deliver helper signals by direct contact with B cell CD 40 ; and XSCID , by mutations in the gamma chain of the lymphocyte receptor for IL 2 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand gene defects responsible for 10 linked hyper IgM syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Impairment of antigen specific T cell priming in mice lacking CD 40 ligand . ^^^ Lack of functional expression of CD 40 ligand ( CD40L ) on T cells results in hyper IgM syndrome ( HIGM 1 ) , a human immunodeficiency associated with a severely impaired humoral immune response that is consistent with defects in B cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Suppression of apoptosis in normal and neoplastic human B lymphocytes by CD 40 ligand is independent of Bc 1 2 induction . ^^^ The tendency of isolated germinal center ( GC ) B cells to undergo apoptosis was suppressed by recombinant cell bound CD 40 ligand ( CD40L ) : after 2 days at 37 degrees C , > 80 % of cells remained viable in the presence of CD40L as compared to < 1 % in control cultures . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cells provide help by making lymphokines , some of which , like CD 40 ligand , remain T cell associated and require cell contact for function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This was done apparently without expressing the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The lack of CD 23 also did not significantly alter in vitro the proliferative response of B cells triggered via the Ag receptor in combination with CD 40 ligand , IL 2 , and / or IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A single strand conformation polymorphism study of CD 40 ligand . ^^^ Mutations in the gene for CD 40 ligand are responsible for the 10 linked form of hyper IgM syndrome . ^^^ To identify efficiently mutations in the gene for CD 40 ligand , eight pairs of PCR primers that could be used to screen genomic DNA by single strand conformation polymorphism ( SSCP ) were designed . 11 different mutations were found in DNA from all 13 patients whose activated T cells failed to bind a recombinant CD 40 construct . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The second signal is typically delivered upon engagement of CD 40 on B cells by the CD 40 ligand expressed on T cells , and results in switching and production of IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Notably , the nuclei of reactive CD3+ / CD4+ T cells nearby to and rosetting around L & H cells in NLPHD were also strongly BCL 6+ , but lacked CD 40 ligand ( CD40L ) expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 on mouse cells was investigated comparing the binding to cells of a monoclonal antibody against CD 40 , to that of a soluble fusion protein consisting of the extracellular domains of the mouse CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Trimeric soluble recombinant human CD 40 ligand ( sCD40L ) was able to inhibit apoptosis induced by the combination of agonists to some degree , but such rescue proved to be short lived . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand transduced co stimulation of T cells in the development of helper function . ^^^ Mice that lack either CD 40 ( expressed on B cells ) or CD 40 ligand ( expressed on activated T cells ) are able neither to make IgG , IgA or IgE antibody responses , nor to generate germinal centres ( the sites of formation of memory B cells ) . ^^^ Here we show that the failure to signal T cells through CD 40 ligand is an important contributory cause . ^^^ Administration of soluble CD 40 in vivo to CD 40 knockout mice , restoring the missing signal through CD 40 ligand initiates germinal centre formation . ^^^ These results indicate that co stimulation of T cells through CD 40 ligand causes their differentiation into cells that help B cells to make mature antibody responses and to generate memory populations . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , ligation of CD 40 by antibody or soluble gp 39 ( CD 40 ligand ) leads to activation of the c Jun kinase ( JNK ) / stress activated protein kinase pathway . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The 10 linked form of hyper IgM syndrome ( HIGM 1 ) is a rare disorder characterized by the inability of B cells to undergo isotype switch by a deficiency of CD 40 ligand ( CD40L ) on activated T lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expression of CD 26 and CD 40 ligand is mutually exclusive in human T cell non Hodgkin ' s lymphomas / leukemias . ^^^ CD 26 and CD 40 ligand ( CD40L ) are surface molecules on human activated T lymphocytes that play a critical role in the regulation of lymphopoiesis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is expressed and functional on activated neonatal T cells . ^^^ Deficient CD 40 ligand ( CD40L ) expression by neonatal T cells has been proposed to explain the diminished Ig production by newborns . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD 40 ligand interactions stimulate B cell antigen processing . ^^^ The interactions between B cell CD 40 and T cell CD 40 ligand ( CD40L ) have been shown recently to play an important role in T cell dependent activation of B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When stimulated in the presence of interleukins 4 and 5 by either lipopolysaccharide or CD 40 ligand , the frequency of IgG 1 precursors in the B1a population was at most one third that of IgM precursors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Study of CD 40 ligand expression in B cell chronic lymphocytic leukemia . ^^^ CD 40 ligand ( CD40L ) is a membrane molecule that plays a key role in T cell B cell cooperation , providing B cells the helper signals needed for activation , proliferation , differentiation and prevention of apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Genomic structure and PCR SSCP analysis of the human CD 40 ligand gene : its application to prenatal screening for 10 linked hyper IgM syndrome . ^^^ To develop a general method for analysis of the mutation and prenatal diagnosis of 10 linked hyper IgM syndrome ( XHM ) , the human CD 40 ligand ( hCD40L ) gene was cloned and sequenced with special reference to the 5 ' and 3 ' flanking regions and exon / intron boundaries . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rapid induction of a novel costimulatory activity on B cells by CD 40 ligand . ^^^ It is well established that the interaction between B cell expressed CD 40 and T cell expressed CD 40 ligand ( CD40L ) is critical for T cell dependent antibody responses , but the role of this interaction in T cell responses is less clear . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The importance of these manifold responses to CD 40 activation for humoral immunity is exemplified by the inability of boys with 10 linked hyper IgM syndrome to make IgG , IgE or IgA due to a mutation in in the gene coding for CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A crystal structure of an extracellular fragment of human CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Differential expression of CD 40 ligand on T cell subsets . ^^^ The CD 40 ligand , an inducible T cell surface molecule , is expressed at higher levels on CD45RA+ T cells as compared with CD45RO+ T cells following CD 3 stimulation . ^^^ Finally , the in vitro conversion of CD45RA+ T cells to the CD45RO+ phenotype is accompanied by a loss in the ability of these cells to express the CD 40 ligand in response to anti CD 3 stimulation as well as a loss in their ability to provide IgE help . ^^^ These results suggest that both CD 45 subsets may play significant and distinct roles in the induction of IgE production under physiologic conditions : CD45RO+ T cells provide IL 4 and the CD45RA+ subset provides the second signal via the CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Treatment of thymocytes with the Ca2+ ionophore ionomycin and the phorbol ester PMA or with anti CD 3 mAb resulted in up regulation of the CD 40 ligand , suggesting that this molecule is involved in CD 40 mediated costimulation of human thymocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These observations suggest previously unforeseen interactions between eosinophils and cells expressing the CD 40 ligand and , thus , novel pathways by which eosinophils may contribute to the regulation of airway inflammation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Endothelial cells augment the expression of CD 40 ligand on newly activated human CD4+ T cells through a CD2 / LFA 3 signaling pathway . ^^^ Cultured human endothelial cells ( EC ) increase CD 40 ligand expression on polyclonally activated human peripheral blood CD4+ helper T cells compared to T cells activated in the absence of accessory cells or in the presence of peripheral blood adherent cells or B cells . ^^^ Induction of CD 40 ligand expression appears to be biphasic with early induction observable at 6 h and later induction at 24 h . ^^^ EC cause T cells to increase CD 40 ligand expression during the early phase at 6 h after activation . ^^^ CD 40 ligand expression is restricted to the CD4+ helper T cell subset of the peripheral blood T cells , even when EC is present . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cleavage of membrane bound CD 40 ligand is not required for inducing B cell proliferation and differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cross linking of the CD 40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up regulates IL 4 synthesis . ^^^ To examine this issue , we established an APC independent system to activate highly purified T cells and induce cytokine synthesis , using immobilized mAbs against several T cell surface molecules , including CD 3 , CD 28 , and the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This inhibition was also observed when cells were either cultured in the `` CD 40 system , ' ' where the anti CD 40 mAb has been immobilized on fibroblasts expressing Fc receptors or in the presence of a soluble chimeric CD 40 ligand molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Levels of mRNA encoding CD 40 ligand was also undetectable under similar conditions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Hyperexpression of CD 40 ligand by B and T cells in human lupus and its role in pathogenic autoantibody production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
All the T cell clones expressed the alpha / beta T cell receptor and were induced to express CD 40 ligand after activation with phorbol myristate acetate plus ionomycin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that fixed cells and the cell membranes from a T cell line , BMS 2 , that expresses high levels of the CD 40 ligand gp 39 ( also called TRAP , TBAM , or CD40L ) stimulate both the expression of mRNA and the production of MMPs by human monocytic cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To study the role of CD 40 ligand ( CD40L ) in the host immune responses against intracellular pathogens , we infected CD40L knockout ( CD40L / ) mice with Leishmania amazonensis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is required for protective cell mediated immunity to Leishmania major . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increase of intracellular calcium is the essential signal for the expression of CD 40 ligand . ^^^ CD 40 ligand ( CD40L ) is present on activated but not on resting T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) is a glycoprotein expressed on the surface of activated helper T cells , basophils , mast cells , and eosinophils . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Four nuclear factor of activated T cells ( NF AT ) binding motifs were found in the murine CD 40 ligand promoter . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Sorted CD38+CD45RA MM cells secreted significantly more TGF beta 1 ( 8 . 2 + / 2 . 0 ng / mL ) than peripheral blood mononuclear cells ( P < . 001 ) , splenic B cells ( P < . 001 ) , and CD 40 ligand ( CD40L ) pretreated B cells ( P < . 05 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cognate T cell help and cross linking of the surface Ig were required , whereas the addition of lipopolysaccharide or a CD 40 ligand to drive proliferation was insufficient . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interaction between CD 40 on B cells and CD 40 ligand molecules on T cells is pivotal for the generation of a thymus dependent antibody response . ^^^ These results suggest that CD 40 engagement by CD 40 ligand expressed on antigen activated T cells is critical for the upregulation of B 7 molecules on antigen presenting B cells that subsequently deliver the costimulatory signals necessary for T cell proliferation and differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand induces expression of CD 25 and CD 23 in resting human tonsillar B lymphocytes . ^^^ In this report , we describe the dose dependent increase in both CD 25 and CD 23 levels on resting human B cells in response to CD 40 ligation , as mediated by soluble CD 40 ligand ( sCD40L ) or anti CD 40 antibody . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Moreover , after activation , these clones expressed on their surface membrane both CD 30 and CD 40 ligand , but not the product of lymphocyte activation gene ( LAG ) 3 , and provided strong helper activity for IgE synthesis by allogeneic B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human dendritic cells activate T lymphocytes via a CD 40 : CD 40 ligand dependent pathway . ^^^ The CD 40 : CD 40 ligand ( CD40L ) interaction provides T lymphocyte mediated help for B lymphocyte and monocyte function but has also been shown to serve as a co stimulus for T lymphocyte activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Flow cytometry of undifferentiated and terminally differentiated KC indicated that both cell types expressed CD 40 , as determined by binding to monoclonal antibodies and a recombinant CD 40 ligand fusion protein ; interferon gamma ( IFN gamma ) treatment of KC increased CD 40 expression . ^^^ These data support the concept that KC , via their expression of CD 40 , have the capacity to amplify inflammation in the skin by interacting with CD 40 ligand bearing T cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Initial studies reported here indicated that stimulation of highly purified peripheral blood B cells with the polyclonal B cell activators Staphylococcus aureus and CD 40 ligand ( CD40L ) resulted in the rapid induction of phosphatidylinositol 3 kinase ( PI 3 kinase ) activity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Demonstration of functional CD 40 in B lineage acute lymphoblastic leukemia cells in response to T cell CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of mouse B cells with anti IgM or IgD antibodies , bacterial lipopolysaccharide , phorbol 12 myristate 13 acetate plus ionomycin , or CD 40 ligand led to a five fold to 35 fold decrease in BCL 6 mRNA levels . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 30 ligand and CD 40 ligand regulate CD 54 surface expression and release of its soluble form by cultured Hodgkin and Reed Sternberg cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To better understand the molecular nature of the TCDD induced suppression of B cell differentiation , we studied the effects of TCDD using in vitro models of T independent ( antibody directed against surface IgM ) and T dependent [ activated T helper ( TH ) cells bearing CD 40 ligand ] B cell maturation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand deficiency presenting as unresponsive neutropenia . ^^^ Investigations showed hypogammaglobulinaemia with normal serum IgM and a novel deletion in the gene for CD 40 ligand on his 10 chromosome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of CD 40 ligand expression on naive CD 4 T cells : a role for TCR but not co stimulatory signals . ^^^ We have investigated the roles of TCR and accessory co stimulatory signals in the induction of CD 40 ligand ( CD40L ) on CD 4 cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro immunization of naive mouse B cells : establishment of IgM secreting hybridomas specific for souble protein or hapten from B cells cultured on CD 40 ligand transfected mouse fibroblasts . ^^^ The natural partner for CD 40 is CD 40 ligand , gp 39 , which is transiently expressed on activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The signals appear to be delivered by GC T cells , require CD 40 ligand expression and may induce differentiation to memory cells . ^^^ Conversely , GC B cells rapidly adopt a memory B cell phenotype on culture with autologous memory CD 4 ( + ) T cells that have been induced to express CD 40 ligand transiently . ^^^ This effect is prevented by blocking CD 40 ligand . ^^^ Naive CD 4 ( + ) T cells , induced to express CD 40 ligand , do not prevent GC B cells undergoing apoptosis . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
By contrast , IL 10 strongly inhibited the transforming growth factor beta mediated generation of mIgA+ cells and Smu Salpha DNA rearrangement events in LPS , but not CD 40 ligand ( CD40L ) activated B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Most Th 2 clones , when activated , produce IL 4 and express CD 40 ligand ( CD40L ) on their cell surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Optimal growth of these cells in vitro depends on CD 40 ligand , T cell cytokines , and a fibroblast stroma , a combination possibly mimicking the dark zone microenvironment . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 with soluble CD 40 ligand reverses anti immunoglobulin mediated negative signalling in murine B lymphoma cell lines but not in immature B cells from neonatal mice . ^^^ Analysis of the most effective signals and timing for inducing this protection suggested that it might , in part , be mediated by CD 40 ligand ( CD40L ) expressed on or secreted by activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Freshly isolated B cells were cultured in vitro in the presence of IL 4 and of L cells expressing CD40L , the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Protective antibodies in murine Lyme disease arise independently of CD 40 ligand . ^^^ To further investigate the role of B . burgdorferi specific Abs in Lyme borreliosis , CD 40 ligand deficient ( CD40L deficient ) mice were infected with B . burgdorferi . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Autoimmune lpr / lpr mice deficient in CD 40 ligand : spontaneous Ig class switching with dichotomy of autoantibody responses . ^^^ To investigate the necessity for T B cell contact in MRL autoimmunity , mice deficient in CD 40 ligand ( CD40L ) were backcrossed onto this background , and Ab synthesis was assessed . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Freshly prepared and in vitro activated CD45RO and CD45RA T cells from atopic patients showed an increased expression of CD 40 ligand when compared with nonatopic individuals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Maintenance of nuclear factor kappa B / Rel and c myc expression during CD 40 ligand rescue of WEHI 231 early B cells from receptor mediated apoptosis through modulation of 1 kappa B proteins . ^^^ Costimulation with CD 40 ligand ( CD40L ) has been shown to rescue WEHI 231 cells from anti sIgM induced apoptosis , and therefore , the effects of CD40L on c myc RNA and protein levels were measured . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The antigenic protein CD 40 on the surface of B lymphocytes plays an important role in their proliferation , immunoglobulin class switching , and rescue from apoptosis in the germinal center through interaction with T lymphocytes expressing CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Therapeutic potential for blockade of the CD 40 ligand , gp 39 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thus , we studied the ability of IFN gamma to regulate Ig secretion in sort purified , resting murine B cells that were > 99 % Ig+ , activated either through membrane Ig using unconjugated or dextran conjugated anti IgD antibodies ( alphadelta dex ) or through CD 40 using soluble or membrane CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent studies using nontransformed B cells and the Ramos Burkitt ' s lymphoma ( BL ) B cell line cells show that CD 40 ligation at the B cell surface by activated , CD 40 ligand ( CD40L ) bearing , CD4+ T cells upregulates Fas expression on B cells and primes B cells for Fas mediated death signals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased expression of CD 40 ligand on systemic lupus erythematosus lymphocytes . ^^^ The specificity of T cell help for B cell activation and differentiation is maintained by the brief expression on the T cell surface , following T cell receptor mediated triggering , of CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The binding of CD 40 ligand on activated T cells to CD 40 on resting B cells induces the expression of costimulatory molecules B 7 1 ( CD 80 ) and B 7 2 ( CD 86 ) . ^^^ The induction of B 7 molecules by CD 40 ligand CD 40 interaction represents a critical step in rendering B cells competent for antigen presentation . ^^^ We have compared the ability of murine CD 40 ligand to induce B 7 1 and B 7 2 expression on B cells isolated from CBA / N and wild type CBA / J mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transient subversion of CD 40 ligand function diminishes immune responses to adenovirus vectors in mouse liver and lung tissues . ^^^ In this paper , we present a strategy for preventing CD4+ T cell activation by an adenovirus vector delivered to mouse liver and lung tissues which is based on interfering with T cell priming via CD 40 ligand CD 40 interactions . ^^^ Adenovirus transgene expression was stabilized in mice genetically deficient in CD 40 ligand ( CD40L ) , and neutralizing antibody to adenovirus did not develop , allowing efficient readministration of vector . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand triggers interleukin 6 mediated B cell differentiation . ^^^ Recently , CD 40 ligand ( CD40L ) , with the co stimulatory cytokines IL 4 and IL 10 , has been shown to trigger immunoglobulin ( Ig ) secretion and class switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation and homologous desensitization of human endothelial cells by CD 40 ligand , tumor necrosis factor , and interleukin 1 . ^^^ We have reported previously that activation of human umbilical vein endothelial cells ( HUVECs ) through CD 40 , using a recombinant soluble form of trimerized CD 40 ligand , leads to induction of E selectin , vascular cell adhesion molecule 1 ( VCAM 1 ) , and intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Here , we compare the effects of CD 40 ligand with those of tumor necrosis factor ( TNF ) and interleukin 1 ( IL 1 ) . ^^^ Quantitatively , TNF is more potent than IL 1 , which is much more potent than CD 40 ligand . ^^^ TNF and CD 40 ligand each induced activation of the transcription factors NF kappa B , IRF 1 , and ATF 2 / c Jun , measured by electrophoretic mobility shift assays , but this response appeared quantitatively similar . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TRAF 2 action is required for activation of the transcription factor NF kappaB triggered by TNF and the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A crystal structure of an extracellular fragment of human CD 40 ligand . ^^^ BACKGROUND : The CD 40 ligand ( CD40L ) is a member of the tumor necrosis factor ( TNF ) family of proteins and is transiently expressed on the surface of activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interaction between CD 40 on B cells and CD 40 ligand ( CD40L ) on T cells has been shown to mediate T cell contact help for B cell proliferation , differentiation , and immunoglobulin isotype switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti Ig down regulates Fas expression of CD 40 ligand stimulated germinal center B cells and inhibits Fas mediated apoptosis . ^^^ The roles for CD 40 ligand ( CD40L ) and T cell derived cytokines in the differentiation of GC B cells were investigated in our culture system . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Reciprocal regulation of mucosal surface IgA+ B cells by Ig receptor cross linking and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 mediated pathway indicates that IL 12 production is induced in DC upon interaction with activated , CD 40 ligand expressing helper T cells , even in the absence of cognate antigen recognition . ^^^ Side by side comparison of IL 12 production , and blocking experiments employing an anti CD 40 ligand mAb , suggest that the CD 40 mediated pathway is quantitatively more significant than induction via the MHC class 2 molecule . ^^^ The importance of the CD40 / CD40 ligand interaction for IL 12 induction in DC likely contributes to the recent finding that mice lacking the CD 40 ligand are impaired in mounting Th 1 type cell mediated immune responses . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon stimulation , some IL 4 producing murine CD8+ T cell clones express CD 40 ligand ( CD40L ) , and they potentiate proliferation and immunoglobulin secretion by small resting B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent studies have suggested that CD 30 and CD 40 ligand ( gp 39 ) on thymocytes is also involved in a part of negative selection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Defective T cell activation may lead to an impairment in cognate T B cell interaction due to impaired expression of CD 40 ligand and / or abnormalities in the production T cell derived cytokines required for fully functional B cell activation , proliferation and / or differentiation which could indeed explain the impairment in antibody production present in CVID patients . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functional CD 40 ligand expression on T lymphocytes in the absence of T cell receptor engagement : involvement in interleukin 2 induced interleukin 12 and interferon gamma production . ^^^ Most interestingly , anti CD 40 ligand ( CD40L ) monoclonal antibody significantly inhibited IL 2 induced IL 12 as well as IFN gamma production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Given the defect in switching to IgG , IgA and IgE isotypes in mice and humans that carry mutations in the CD 40 and CD 40 ligand genes , we have investigated the role of CD 40 ligation in controlling B cell responses to interleukin ( IL ) 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Multimerization of CD 40 molecules on B cells by binding CD 40 ligand on activated T cells initiates signaling events essential for B cell differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand dependent T cell activation : requirement of B 7 CD28 signaling through CD 40 . ^^^ The role of CD 40 ligand ( CD40L ) in the primary activation of T cells is not clear . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Requirement for CD 40 ligand in costimulation induction , T cell activation , and experimental allergic encephalomyelitis . ^^^ The mechanism of CD 40 ligand ( CD40L ) mediated in vivo activation of CD 4 ( + ) T cells was examined by investigation of the development of experimental allergic encephalomyelitis ( EAE ) in CD40L deficient mice that carried a transgenic T cell receptor specific for myelin basic protein . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These genes include those required for B cell help such as interleukin ( IL 4 ) and CD 40 ligand as well as those necessary for T cell proliferation such as IL 2 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 4 induced Fas resistance is not associated with an alteration in the elevated level of Fas expression produced by CD 40 ligand and does not depend on additional receptor mediated signals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand ( CD40L ) : CD 40 interaction plays an important role in the activation of both T and B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Further studies designed to elucidate the mechanism by which wortmannin treated T cells failed to provide B cell help indicated that wortmannin and LY 294002 significantly inhibited the induction of CD 40 ligand and , to a lesser extent , intercellular adhesion molecule 1 expression . ^^^ These results suggest that the PI 3 kinase signaling pathway , or other wortmannin and LY 294002 sensitive pathways , may be important for the induction of expression of crucial interaction molecules , such as CD 40 ligand , on T cells and thus indicates that D 3 phosphoinositides play a pivotal role in regulating T cell dependent B cell activation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Binding of CD 40 ligand to CD 40 on a murine B lymphoma M 12 induces B 7 1 and inhibits cell growth . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti CD 40 ligand antibody treatment prevents the development of lupus like nephritis in a subset of New Zealand black 10 New Zealand white mice . ^^^ The interaction between CD 40 on B cells and CD 40 ligand ( CD40L ) on Th cells is necessary for normal thymus dependent Ab production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this review , an overview of the CD 40 antigen and the CD 40 ligand is given , focussing on their possible role in B cell malignancies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Although CD 40 ligand ( CD40L ) triggers a shift from dephosphorylated to phosphorylated pRB and proliferation of B cells , the addition of exogenous IL 6 to CD40L treated B cells does not alter either pRB or proliferation , as observed in MM cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , SIC4 . 8R labeling declined following the stimulation of ileal PP B cells with CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A central role of CD 40 ligand in the regulation of CD4+ T cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Wild type BTK was necessary for proliferation induced by soluble anti IgM ( a prototype for thymus independent type 2 antigen ) , but not for responses to soluble CD 40 ligand ( CD40L , the B cell activating ligand expressed on T helper cells ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The anergized T cells also failed to give B cells help in IgE synthesis , although they expressed normal levels of the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These observations suggest that IL 10 has a role in the suppression of inappropriate B cell proliferation , i . e . , proliferation by B cells that have not effectively interacted with relevant Ag or CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Therefore , we tested the ability of B cells from relB knockout mice ( relB / ) to proliferate , undergo maturation to IgM secretion , and switch to the expression of downstream Ig isotypes in response to distinct activators including LPS , anti CD 40 mAb or CD 40 ligand , and / or dextran anti IgD antibodies in combination with various cytokines , including IL 4 , IL 5 , IFN gamma , and TGF beta . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mutation analysis in CD 40 ligand deficiency leading to 10 linked hypogammaglobulinemia with hyper IgM syndrome . ^^^ Mutations in the gene encoding CD 40 ligand have been shown to be the cause of 10 linked hypogammaglobulinemia with hyper IgM ( HIGM 1 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Herpesvirus saimiri transformed human CD4+ T cells can provide polyclonal B cell help via the CD 40 ligand as well as the TNF alpha pathway and through release of lymphokines . ^^^ However , the surface expression of the two cell lines differed in that CHCD 4 constitutively expressed CD 40 ligand ( CD40L ) and membrane TNF alpha , but MHCD 4 did not . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Delayed CTLA4Ig administration was unable to alter acute GVHD but did reverse chronic GVHD as evidenced by normalization of serum autoantibody levels , normal host B cell numbers and MHC class 2 expression , reduced donor T cell expression of CD 40 ligand , and reduced numbers of donor CD4+ memory T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , ligation of CD 40 via soluble gp 39 , the CD 40 ligand , enhances intercellular adhesion molecule ( ICAM ) 1 and Bcl 10 up regulation on IFN gamma stimulated KC , but not lymphocyte function associated antigen ( LFA ) 3 , B 7 2 , HLA DR , or Fas expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the course of cognate interaction between CD 40 ligand ( CD40L ) bearing CD4+ T cells and CD 40 expressing germinal center B cells , CD 40 ligation results in augmented Fas expression at the B cell surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Next , we triggered the CD 40 antigen by anti CD 40 antibodies and by a CD 40 ligand expressing cell line . ^^^ Stimulation via the CD 40 ligand was more successful than using crosslinked anti CD 40 antibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functional CD 40 ligand expressed by human blood dendritic cells is up regulated by CD 40 ligation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Molecular pathology of the 10 linked hyper immunoglobulin M syndrome : detection of wild type transcripts in a patient with a complex splicing defect of the CD 40 ligand . ^^^ Recently , a B cell surface antigen ( CD 40 ) and its conjugate T cell counterstructure ( CD 40 ligand ) were shown to mediate immunoglobulin isotype switching in the presence of cytokines such as interleukin 4 . ^^^ Most patients with XHIM have been shown to have mutations of the extracellular domain of the CD 40 ligand . ^^^ Here we describe a novel point mutation of an intronic splice acceptor site which results in a complex splicing defect of the CD 40 ligand in a patient with XHIM . ^^^ The demonstration of wild type CD 40 ligand transcripts may be an explanation for previous observations suggesting that some XHIM patients are able to undergo immunoglobulin isotype switching in vivo . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The superantigen toxic shock syndrome toxin 1 induces CD 40 ligand expression and modulates IgE isotype switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Encouraging results were obtained when vector was administered with one of several immune modulating agents including cyclophosphamide , mAb to CD4+ cells , and mAb to CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activated T hybridomas induce upregulation of B 7 1 on bystander B lymphoma cells by a contact dependent interaction utilizing CD 40 ligand . ^^^ Antibodies to the CD 40 ligand ( CD40L ) of T cells inhibited the increased appearance of B 7 1 on B lymphomas . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Costimulatory function and expression of CD 40 ligand , CD 80 , and CD 86 in vascularized murine cardiac allograft rejection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Peptide induced up regulation of CD 40 ligand expression at high peptide concentrations showed no difference between the wild type and analogue peptides . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In 29 of 32 MMs showing CD 40 in the vertical growth phase , expression of the CD 40 ligand ( CD40L ) was studied ; in 13 of these 29 , CD40L was found in the same tumor areas that expressed CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of cognate and non cognate T cell help for B cell IgE production in relation to CD 40 ligand expression . ^^^ We demonstrated that the T cells neither expressed detectable CD 40 ligand at the beginning of the superantigen treatment nor 24 h later . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40lbase : a database of CD40L gene mutations causing 10 linked hyper IgM syndrome . 10 linked hyper IgM syndrome ( 10 HIM ) is an immunodeficiency caused by mutations in the gene encoding the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand ( CD40L ) is a molecule expressed by activated T cells which plays a critical role in the regulation of B cell responses , including differentiation into Ig producing cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand mRNA expression was also detectable in vivo using reverse transcription PCR in 28 of the 29 ( 97 % ) tumor samples tested , including 11 of those tested for [ 3H ] thymidine incorporation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand deficient mice generate a normal primary cytotoxic T lymphocyte response but a defective humoral response to a viral infection . ^^^ CD 40 ligand is expressed on activated T cells and interacts with CD 40 on B cells and monocytes . ^^^ It is not known what role CD 40 ligand plays in the generation of immune responses to viral infection . ^^^ To address this issue , we examined virus specific T and B cell responses in CD 40 ligand deficient ( CD40L / ) mice following infection with lymphocytic choriomeningitis virus ( LCMV ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we show that human dendritic cells respond to CD 40 ligand , as well as to tumor necrosis factor alpha and IL 1 beta , with intracellular ceramide accumulation , as they are induced to differentiate . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blockade of CD 40 ligand CD 40 interaction impairs CD4+ T cell mediated alloreactivity by inhibiting mature donor T cell expansion and function after bone marrow transplantation . ^^^ Alloreactive T cells require costimulatory signals via CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using CD 40 ligand transfected L cells ( CD40Lc ) , we demonstrated that CD 40 triggering results in an enhanced secretion of both IL 8 and TNF alpha by cultured epidermal basal cells , suggesting that CD 40 CD40L interactions may play a role in amplifying the cutaneous inflammatory reactions . ^^^ In addition , inhibition of proliferation could be obtained by adding a soluble form of CD 40 ligand to the keratinocyte cultures . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate the role of CD 40 ligand in the delivery of help to B cells , we examined the Ag specific interaction of B cells from CD 40 deficient mice with a Th 2 cell line in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 4 and CD 40 ligand stimulate transcription of CD 23 ( Fc epsilonRII ) in B cells and are necessary for the expression of germline epsilon mRNA and production of IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , these results suggest that IL 12 production can be induced by bacterial products in patients with hyper IgM syndrome who lack CD 40 ligand expression and in those treated with soluble gp 39 to interrupt CD 40 CD40 ligand interactions . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction , regulation , and function of soluble TRAP ( CD 40 ligand ) during interaction of primary CD4+ CD45RA+ T cells with dendritic cells . ^^^ To assess the induction , regulation , and the relative roles of cell surface tumor necrosis factor related activation protein ( TRAP ; CD 40 ligand ) and the soluble form of TRAP ( sTRAP ) in the initial phase of T cell activation , primary CD4+ CD45RA+ ( naive ) T cells were co cultured with mature Langerhans ' cells ( mLC ) in the presence of superantigen . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We find that 4 1BBL is inducible on splenic B cells by CD 40 ligand / CD40 interaction or by culturing of splenic dendritic cells , treatments that also induce B 7 family molecules . ^^^ However , treatment of spleen cells with CD 40 ligand renders them competent in activation of CD 28 T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A CD40+ hepatocellular carcinoma cell line , HepG 2 , susceptible to cryptosporidia and CMV infection became resistant when cell surface CD 40 was cross linked by a CD 40 ligand fusion protein . ^^^ We propose that the CD 40 ligand mutations that cause XHIM deprive the biliary epithelium of one line of defense against intracellular pathogens and that malignant transformation in the biliary tree follows chronic infection or inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent in vitro studies have shown that B cells activated by CD 40 ligand become extremely sensitive to Fas mediated apoptosis while IL 4 and / or surface IgM receptor engagement protects B cells from Fas ligand cytolysis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IgG synthesis was not stimulated , however , probably because of lack of CD 40 ligand expression on the infected T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The production of IL 12 by macrophages / dendritic cells ( Mphi / DC ) is mediated either by a T cell dependent pathway that is induced primarily by the interaction of CD 40 ligand ( CD40L ) on activated T cells with CD 40 on IL 12 producing cells or by a T cell independent pathway that is induced by bacteria or bacterial products and enhanced by interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD 40 ligand in costimulation and T cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased interleukin 12 production in progressive multiple sclerosis : induction by activated CD4+ T cells via CD 40 ligand . ^^^ Anti CD 40 ligand antibody completely blocked IL 12 secretion induced by activated T cells , and we found increased CD 40 ligand expression by activated CD4+ T cells in MS patients vs . controls . ^^^ The CD 40 ligand dependent Th 1 type immune activation was observed in the progressive but not in the relapsing remitting from of MS , suggesting a link to disease pathogenesis and progression and providing a basis for immune intervention in the disease . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
As hyper IgM patients with mutated CD 40 ligand ( CD40L ) exhibit neutropenia , no B cell memory , and altered T cell functions leading to severe infections , we investigated the potential role of CD 40 on CD34HPC development . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After the BMT , mice were treated with or without 2 6 microg of a soluble recombinant murine CD 40 ligand ( srmCD40L ) given intraperitoneally twice a week . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We present a boy with hyper IgM syndrome with a previously not reported mutation in the CD 40 ligand gene . ^^^ Thus , it is possible that both the neutropenia and the NK cell deficiency are due to lack of growth promoting signals normally delivered by the CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , the author reviews recent evidence demonstrating that inhibition of c myc expression activates apoptosis of B cell models of clonal deletion and that rescue from apoptosis by CD 40 ligand leads to maintenance of c Myc levels . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cell regulation and CD 40 ligand dependence in murine systemic autoimmunity . ^^^ To explore the mechanisms by which alpha beta T cells and gamma delta T cells regulate systemic autoimmunity , lupus prone mice were rendered deficient in CD 40 ligand and / or alpha beta T cells by intercrossing CD40L / and TCR alpha / knockouts , generating CD40L intact or deficient ( CD40L+ or CD40L ) , alpha beta T cell intact or deficient ( alpha beta+ or alpha beta ) MRL lpr / lpr animals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Features of the CD 40 ligand binding site are discussed in conjunction with the results of a previous mutagenesis study . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B 1 cells expressed a similar level of CD 40 as that on B 2 cells , and proliferated in response to a soluble CD 40 ligand ( CD40L ) CD8alpha chimeric protein , suggesting that CD 40 on B 1 cells is functional . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand . ^^^ For several years , the primary function of CD 40 ligand ( CD40L ) has been believed to be in regulation of contact dependent , CD 40 CD40L mediated signals between B and T cells , which are essential for the regulation of thymus dependent ( TD ) humoral immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , we found that the pathogenic Th cells and B cells of lupus have a regulatory defect in the expression of CD 40 ligand ( CD40L or gp 39 ) , which results in abnormal costimulatory signals that sustain the production of pathogenic autoantibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cross linking of B cell immunoglobulin ( Ig ) receptors by antigen , stimulation of the integrin adhesion molecules LFA 1 and VLA 4 on the B cell through interaction with their counter receptors ICAM 1 and VCAM 1 on the FDC and cross linking of CD 40 on the B cells through interaction with the CD 40 ligand ( CD40L ) on T cells have been shown to prevent entry into apoptosis of GC B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Exogenous CD 40 ligand induces a pulmonary inflammation response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Moreover , BCR cross linking in Lyn / B cells suppressed Fas expression induced by costimulation with CD 40 ligand and IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction between CD 40 ligand and CD 40 is critical for activation of T and B cells in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate , whether CD 40 is functionally active there , we investigated the proliferative response by 3H Thymidin incorporation of primary normal and neoplastic thymic epithelial cell cultures to CD 40 triggering by soluble CD 40 ligand ( CD40L ) . ^^^ RESULTS : Normal thymic epithelial cells exhibited proliferation in response to CD 40 ligand in a dose dependent manner , while a CD 40 ligand specific monoclonal antibody prevented this effect . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functional CD 40 ligand is expressed on human vascular endothelial cells , smooth muscle cells , and macrophages : implications for CD 40 CD40 ligand signaling in atherosclerosis . ^^^ We report here that CD 40 ligand ( CD40L ) , an immunoregulatory signaling molecule heretofore considered largely restricted to recently activated CD4+ T lymphocytes , is expressed by human vascular endothelial cells ( EC ) , smooth muscle cells ( SMC ) , and human macrophages in vitro , and is coexpressed with its receptor CD 40 on all three cells types in human atherosclerotic lesions in situ . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Apoptosis was induced in these transfectants by soluble CD 40 ligand and also by the anti CD 40 monoclonal antibodies ( mAb ) M 2 and M 3 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Allergen specific IgE was induced by simultaneously activating T cells and B cells specifically with allergen and polyclonally with anti CD 2 and soluble CD 40 ligand in the presence of IL 4 . ^^^ In peripheral blood mononuclear cell cultures stimulated with soluble CD 40 ligand , IL 4 , and phospholipase A 2 , stimulation of T cells with higher amounts of anti CD 2 enhanced IgG 4 in parallel to increased IL 2 and interferon gamma secretion but inhibited IgE synthesis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Abnormal CD 40 mediated activation pathway in B lymphocytes from patients with hyper IgM syndrome and normal CD 40 ligand expression . ^^^ The CD 40 mediated activation pathway of B cells from 10 patients with hyper IgM syndrome and normal expression of CD 40 ligand was studied . ^^^ Altogether , these results suggest that CD 40 ligand positive hyper IgM syndrome could be genetically heterogeneous , although phenotypic variability is not excluded , and that an early defect in the CD 40 triggered activation cascade can account for defective Ig class switching in some patients with CD 40 ligand positive hyper IgM syndrome . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
No change in CD 40 ligand expression was observed . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 4 activates resting B cells and , in conjunction with cosignals such as anti IgM ( anti mu ) Ab or CD 40 ligand , modulates progression of B cells through the cell cycle , leading to proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 on the immature mouse B cell line WEHI 231 with recombinant CD 40 ligand ( CD40L ) was found to protect cells from apoptosis after gamma irradiation , as well as that following treatment with the sphingomyelin ceramide or compounds that deplete intracellular glutathione . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , B cells purified from thymus that had been inoculated with B cell progenitors were able to differentiate into Ab forming cells under the influence of CD 40 ligand plus IL 10 , and secreted IgM with donor Igh 6 allotype . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 antigen , expressed on many cell types is involved in cell cell interactions , with activated T cells expressing the CD 40 ligand . ^^^ The lack of CD 40 expression by tumor cells might account for tumor escape mechanism from activated T cells expressing the CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 has been clustered as a member of the nerve growth factor ( NGF ) / tumor necrosis factor ( TNF ) receptor superfamily with the corresponding counterstructure , the CD 40 ligand ( L ) being mainly expressed by activated CD4+ T cells , but also some activated CD8+ T cells , basophils , eosinophils , mast cells and stromal cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It expresses CD 4 , TCR alphabeta , Thy 1 . 2 and H 2k on the cell membrane , as well as CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antigens , T cell signals ( cytokines , CD 40 ligand and Fas ligand ) as well as the activation state of B cells determine their ultimate fate . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of trimeric CD 40 ligand in Pichia pastoris : use of a rapid method to detect high level expressing transformants . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cellular responses mediated by CD 40 are naturally triggered by its counter receptor , the CD 40 ligand , which is displayed on activated T cells , mast cells , eosinophils , basophils , and B lineage cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The NF AT family of transcription factors participates in the regulation of early immune response genes such as IL 2 , IL 4 , CD 40 ligand , and Fas ligand in response to Ca2+ / calcineurin signals initiated at the antigen receptor . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Treatment with CD 40 ligand ( CD40L ) , however , greatly enhanced Fas susceptibility of these cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cytotoxicity and weak CD 40 ligand expression of CD8+ type 2 cytotoxic T cells restricts their potential B cell helper activity . ^^^ Additionally , Tc 1 and Tc 2 cells failed to express CD 40 ligand ( CD40L ) , whereas Th 1 and Th 2 cells expressed high levels of CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We used human tonsillar B cells and Burkitt lymphoma cell lines which were activated by CD 40 ligand , surface ( s ) IgM cross linking , or LPS . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of B chronic lymphocytic leukemia cells by murine fibroblasts , IL 4 , anti CD 40 antibodies , and the soluble CD 40 ligand . ^^^ In contrast , the soluble recombinant human CD 40 ligand and two different anti CD 40 antibodies did not prolong cell survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Coculture of resting B cells with simian virus 40 ( SV 40 ) transformed NIH3T3 ( MOP 8 ) cells stably transfected with the ligand for CD 40 ( bovine CD 40 ligand , boCD40L ) , resulted in proliferation which was enhanced by addition of rbo interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Positive and negative effects of CD 40 ligation on human B cell function were suggested by the observation that mAb to CD 40 ligand partially blocked the suppressive influences of anti CD 3 stimulated control CD4+ T cells , as well as the B cell stimulatory effects of anti CD 3 activated mitomycin C treated CD4+ T cells . ^^^ To examine the negative effects of CD 40 ligation in greater detail , B cells were cultured with anti CD 3 activated mitomycin C treated CD4+ T cells that expressed optimal levels of CD 40 ligand ; additional recombinant human CD 40 ligand significantly suppressed Ig production , but not proliferation . ^^^ In contrast , when B cells were stimulated with SAC ( formalinized Cowan 1 strain Staphylococcus aureus ) and IL 2 in the absence of T cells , small amounts of recombinant CD 40 ligand stimulated Ig production , whereas larger quantities directly suppressed Ig secretion . ^^^ Finally , activated B cells acquired the capacity to down regulate CD 40 ligand expression by stimulated CD4+ T cells more effectively than resting B cells . ^^^ These results indicate that during T cell B cell collaboration , engagement of CD 40 can influence Ig production both positively and negatively , depending on the density of CD 40 ligand as well as the stage of B cell activation and differentiation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Signaling through CD 40 with soluble CD 40 ligand stimulated fibroblast activation , as evidenced by mobilization of nuclear factor kappaB and by induction of the proinflammatory and chemoattractant cytokines IL 6 and IL 8 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cell growth and differentiation in cocultures were found to be dependent on the expression of CD 40 ligand ( CD40L ) on the cloned T cells , and the expression of this molecule was only marginally blocked by the induction of anergy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We report in this study that treatment of mouse M12 . 4 . 1 B lymphoma cells with soluble CD 40 ligand ( CD40L ) CD8alpha fusion protein modestly induces the promoter for germline epsilon transcripts , and that this induction synergizes with IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rather , it appears primarily due to an inability of Sezary cells to significantly up regulate CD 40 ligand ( gp 39 ) following in vitro exposure to PHA . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand ( gp 39 ) is transiently expressed on activated CD4+ T cells and mediates cognate helper function by interacting with CD 40 on B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of human IgE synthesis in B cells requires , in addition to IL 4 or IL 13 , a second signal provided by CD 40 ligand ( CD40L ) on activated Th 2 type CD4+ T cells that do not or weakly express Fas ligand ( FasL ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TH cells activate B cells by their products , cytokines such as IL 4 , IL 5 , and IL 6 , and membrane bound stimulatory molecules including CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
VH repertoire in human B lymphocytes stimulated by CD 40 ligand and IL 4 : evidence for positive and negative selection mechanisms coupled to CD 40 activation . ^^^ The specific prediction that the VH repertoire of CD 40 activated B cells would differ from the repertoire of unstimulated cells from the same donor , was tested by assessing VH utilization among human B cell clones grown in vitro , following stimulation with CD 40 ligand ( CD40L ) and IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In an 10 linked immunodeficiency with hyper IgM ( 10 HIM ) , several mutations of genes coding for CD 40 ligand are responsible for B cell defects . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , this locus is near that of the CD 40 ligand gene , the product of which is also involved , like IL 13 , in proliferation and IgE isotype switching of human B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulary organized lymphoid tissue and CD 40 ligand expression in a male fetus carrying the defective gene for 10 linked lymphoproliferative disease ( XLP ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We demonstrate that IL 2 activated human NK cells can express CD 40 ligand ( CD40L ) and that recognition of CD 40 on target cells can provide an activation pathway for such human NK cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mutations of the CD 40 ligand gene in 13 Japanese patients with 10 linked hyper IgM syndrome . 10 linked hyper IgM syndrome ( XHIM ) is a rare primary immunodeficiency caused by a defective CD 40 ligand . ^^^ We identified mutations of the CD 40 ligand gene in 13 unrelated Japanese XHIM patients . ^^^ Three patients had nonsense mutations , all of which resulted in the truncation of the TNF homology domain of the CD 40 ligand . ^^^ These results indicate that mutations in XHIM are highly heterogeneous , although codon 140 seems to be a hot spot of the CD 40 ligand gene since two additional point mutations were located at Trp 140 , bringing the total numbers of mutations affecting codon 140 to six . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD4+ cells from patients with Common Variable Immunodeficiency have a reduced ability of CD 40 ligand membrane expression after in vitro stimulation . ^^^ Recently , it has been reported that the expression of the CD 40 Ligand ( CD40L ) , a T cell surface molecule that plays a critical role in the cell contact mediated helper signals provided to B cells , is defective in a subset of patients with CVID . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interleukin 13 in combination with CD 40 ligand potently inhibits apoptosis in human B lymphocytes : upregulation of Bcl xL and Mcl 1 . ^^^ The inhibition of cell death by IL 13 alone was significant but modest , but was potently enhanced in combination with CD 40 ligand ( CD40L ) , a survival stimulus for B cells by itself . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antigen specificity of dual reactive T hybridomas determines the requirement for CD 40 ligand CD 40 interactions . ^^^ CD 40 ligand ( CD40L ) expression on T cells is known to play a crucial role in B cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , it has been established that ligation of CD 40 ligand ( CD40L ) delivers signals to the CD40L bearing cells themselves . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 30 ligand and CD 40 ligand expression in lymph nodes involved with Hodgkin ' s disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Notably , the nuclei of reactive CD3+ / CD4+ T cells near to and rosetting around L & H cells in NLPHD were also strongly bcl 6+ , but lacked CD 40 ligand ( CD40L ) expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Previous studies have demonstrated that the interaction of CD 40 on monocytes with CD 40 ligand , present on activated CD4+ T cells , induces monocyte inflammatory cytokine synthesis and rescues monocytes from apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antibody to CD 40 ligand inhibits both humoral and cellular immune responses to adenoviral vectors and facilitates repeated administration to mouse airway . ^^^ Here we demonstrate that in mice , a transient blockade of costimulation between activated T cells and B cells / antigen presenting cells using a monoclonal antibody ( MR 1 ) against murine CD 40 ligand inhibits the development of neutralizing antibodies to adenoviral ( Ad ) vector . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Engagement of the B cell antigen CD 40 by the CD 40 ligand ( CD40L ) expressed on T cells leads to subsequent isotype switching during immunoglobulin synthesis in B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
On tonsil B cells , ligation of CD 40 by CD 40 ligand , induces a transient strong upregulation of expression of the c Met tyrosine kinase . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Moreover , CD 40 ligand expression by activated B cells is likely to play an essential role in facilitating ongoing responses of stimulated B cells maturing in germinal centers . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunoglobulin ( Ig ) E production by B cells requires two primary signals provided by T cells , interleukin ( IL ) 4 or IL 13 and CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Trimeric leucine zipper peptides and the trimeric C terminal domain of CD 40 ligand were shown to inhibit RAM 1 mediated infection of NIH3T3 cells by the 4070A envelope when fused to its N terminus , whereas monomeric helical peptides and the monomeric epidermal growth factor domain did not . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We report here that stimulation of human vascular SMC and endothelial cells ( EC ) through CD 40 ligand , a mediator recently localized in human atheroma , induced elaboration of the IL 1beta precursor as well as activation of cell associated ICE . ^^^ In addition to the constitutively expressed 45 and 30 kDa immunoreactive ICE proteins , vascular cells incubated with recombinant human CD 40 ligand ( rCD40L ) ( but not IL 1 or TNF ) showed an increase of a 20 kDa immunoreactive ICE protein by Western blot analysis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Prolonged survival of mouse skin allografts in recipients treated with donor splenocytes and antibody to CD 40 ligand . ^^^ Combined treatment with antibody against CD 40 ligand and one transfusion of donor splenocytes prolonged survival of fully mismatched BALB / c skin allografts on C57BL / 6 recipients , with approximately 20 % of grafts surviving > 100 days . ^^^ Treatment with donor splenocytes and antibody against CD 40 ligand permits long term survival of highly antigenic donor skin allografts despite the presence of functionally intact alloreactive lymphocytes . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of germinal center B cell markers in vitro by activated CD4+ T lymphocytes : the role of CD 40 ligand , soluble factors , and B cell antigen receptor cross linking . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The ligation of CD 40 on B lymphocytes by CD 40 ligand , transiently expressed on activated Th cells , provides a key activation signal required for the germinal center B cell response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand induction on T cell subsets by peptide presenting B cells : implications for development of the primary T and B cell response . ^^^ Recent data suggest that CD 40 ligand ( CD40L ) CD 40 interactions are essential for up regulation of costimulatory activity on APC and that efficient induction of CD40L may be pivotal to the success of a CD 4 T cell response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study tested the hypothesis that the T lymphocyte surface molecule CD 40 ligand ( CD40L ) , recently localized in atherosclerotic plaques , regulates the expression of MMPs in human vascular smooth muscle cells ( SMCs ) , the most numerous cell type in arteries . ^^^ These results demonstrated that CD 40 ligand , expressed on T lymphocytes , promoted the expression of matrix degrading enzymes in vascular SMCs and thus established a new pathway of immune modulated destabilization in human atheromata . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Basic immunological research has benefited from the use of recombinant viruses to further understand the role of molecules such as CD 40 ligand , nitric oxide and interleukin 4 . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Acquired CD 40 ligand deficiency in chronic lymphocytic leukemia . ^^^ Patients with B cell chronic lymphocytic leukemia ( CLL ) acquire an immunodeficiency with many characteristics similar to those of persons with inherited defects in the gene encoding the CD 40 ligand ( CD 154 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Saturating concentrations of CD 40 ligand , which fully inhibit naive B cell differentiation , only partially affect that of memory B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
For this purpose , DC generated by culturing plastic adherent peripheral blood mononuclear cells for 7 days with granulocyte / macrophage colony stimulating factor and IL 4 were incubated for 3 days with either LPS ( 10 ng / ml ) or 3T6 fibroblasts transfected with the gene encoding CD 40 ligand , in absence or presence of IL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T helper cells driving pathogenic anti DNA autoantibody production in lupus : nucleosomal epitopes and CD 40 ligand signals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We selectively mimicked the effect of T cell help by addition to peripheral blood B cells activated with Staphylococcus aureus Cowan 1 strain and IL 2 of irradiated 300 19 cells transfected with either the CD 70 ( CD 27 ligand ) gene or the CD 154 ( CD 40 ligand ) gene , the vector alone , or both CD 70 and CD 154 genes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Each protein forms independent DNA binding complexes , and at physiologic concentrations , NF ATc and NF ATp complexes out compete NF AT4 / x / c3 for occupancy of NF AT sites from the IL 2 , IL 3 / granulocyte macrophage CSF , IL 4 , and CD 40 ligand genes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cells from patients with lupus express increased amounts of the CD 40 ligand , which is functional because it helps B cells to produce anti DNA antibodies and express more CD 80 ( B 7 1 ) on their surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The various lymphocyte types within the adaptive immune system are interdependent , as evidenced by the 10 linked hyper IgM syndrome in which a T cell deficiency ( CD 40 ligand ) manifests clinically as an antibody production problem . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Only two molecules , CD 40 ligand and IL 2 , were found to be extremely dependent on accessory molecule help , with little or no expression evident with peptide presented on resting B cells or class 2 positive fibroblasts . ^^^ Partial activation , however , may be the overall result due to the lack of CD 40 ligand expression , which may regulate costimulatory activity in APC and , in turn , may contribute to limiting the production of IL 2 required for T cell expansion and survival . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand induces an antileukemia immune response in vivo . ^^^ CD 40 ligand ( CD40L ) is a costimulator molecule that directly activates T cells and may promote antigen presentation by CD 40 expressing cells , which include professional antigen presenting cells and B acute lymphoblastic leukemia ( ALL ) cells from many patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand [ CD40L ] , CD 30 , CD 27 , hOX 40 ) have been considered to be either T cell restricted or strongly T cell associated . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) has been shown to increase surface Fas expression and induce B cell sensitivity to Fas dependent CD4+ Th 1 cell mediated cytotoxicity ( Th 1 CMC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
PLA specific Ab were induced by simultaneously activating T cells and B cells specifically with allergen and polyclonally with anti CD 2 and soluble CD 40 ligand ( sCD40L ) in the presence of interleukin ( IL ) 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Differential effects of LPS and CD 40 ligand stimulations on the induction of IL 12 production by dendritic cells and macrophages . ^^^ In this experiment , we have examined the IL 12 production of murine macrophages ( Mphi ) and dendritic cells ( DC ) in response to LPS and CD 40 ligand ( CD40L ) stimulations . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand expressed on activated T cells plays a pivotal role in B cell proliferation and differentiation . ^^^ Mutations in the CD 40 ligand gene , which alter its expression on the surface of activated T cells , are associated with the 10 linked form of Hyper IgM syndrome ( XHIM ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand directly activates T lymphocytes via tyrosine phosphorylation dependent PKC activation . ^^^ Here , we provide evidence that the CD 40 ligand ( CD40L ) also functions as a direct stimulatory molecule for T lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B lymphoma cells expressed the CD 40 ligand ( CD40L ) , and blocking of CD 40 CD40L interactions between HIV infected MVECs and B lymphoma cells inhibited B cell attachment and proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , stimulation via TCR+CD28 resulted in significantly decreased expression of CD 40 ligand in the patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , addition of soluble CD 40 ligand ( L ) synergistically augmented IL 12 production in macrophages and MoDC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro activation of low grade non Hodgkin ' s lymphoma by murine fibroblasts , IL 4 , anti CD 40 antibodies and the soluble CD 40 ligand . ^^^ In contrast , the soluble recombinant CD 40 ligand gp 39 and the anti CD 40 mAbs mAb 89 and EA 5 did not prolong cell survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The central role of the CD 40 ligand and CD 40 pathway in T lymphocyte mediated differentiation of B lymphocytes . ^^^ This review summarizes recent findings concerning the role of CD 40 ligand and CD 40 interactions in B cell differentiation . ^^^ CD 40 ligand on helper CD4+ T lymphocytes interacts with CD 40 on B cells and directs the selection and differentiation of clones of B lymphocytes to generate specific antibody dependent immune responses . ^^^ CD 40 ligand is necessary for normal B cell differentiation and plays several distinctive roles in this multistage process . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand expressed on B cells in the BXSB mouse model of systemic lupus erythematosus . ^^^ In examining the mechanism of the B cell hyperactivity , it was found that 20 % of unstimulated large B cells from male mice , unlike large B cells from female mice , expressed CD 40 ligand ( CD40L ) , a molecule normally expressed on activated CD4+ cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand CD 40 interactions are necessary for the initiation of insulitis and diabetes in nonobese diabetic mice . ^^^ Here , we investigate the role of CD 40 ligand ( CD40L ) CD 40 costimulation in the initiation and progression of this disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antitumor responses induced by transgenic expression of CD 40 ligand . ^^^ Because CD 40 ligand ( CD40L ) is a co stimulator molecule for multiple components of the immune response , we wanted to determine whether transgenic expression of the molecule would increase immune responses against a weakly immunogenic murine tumor , neuro 2a . ^^^ These experiments illustrate that transducing even a small percentage of tumor cells with CD 40 ligand can create a long lasting systemic immune response capable of impeding growth of unmodified neuroblastoma cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When CD 40 is engaged with CD 40 ligand ( CD40L ) , nuclear factor kappaB binding activity is up regulated as is interleukin ( IL ) 6 and IL 8 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Requirement of a second signal via protein kinase C or protein kinase A for maximal expression of CD 40 ligand . ^^^ High levels of CD 40 ligand ( CD40L ) protein expression are induced on native T cells by increasing the intracellular Ca2+ concentration . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upregulation of CD 40 and CD 40 ligand expression in IgE associated cutaneous diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Evidence for a novel function of the CD 40 ligand as a signalling molecule in T lymphocytes . ^^^ Further , cellular stimulation via the CD 40 ligand results in tyrosine phosphorylation of cellular proteins and the activation of p 56 ( lck ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We showed that anti CD 40 mAb or CD 40 ligand acts in synergy with rhIL 4 to enable the transduction of approximately 50 75 % of B CLL cells ( B CLL , n = 6 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vivo microbial stimulation induces rapid CD 40 ligand independent production of interleukin 12 by dendritic cells and their redistribution to T cell areas . ^^^ Importantly , this production of IL 12 occurs very rapidly and is independent of interferon gamma priming or of signals from T cells , such as CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 and CD 40 ligand ( CD 154 ) are coexpressed on microvessels in vivo in human cardiac allograft rejection . ^^^ It binds to CD 40 ligand ( [ CD40L ] CD 154 ) , which was originally reported to be restricted in its expression to early activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Similarly , blockade of CD 40 ligand , which preserves the CD4+ T helper cell population , also improves expression over time ( 30 % of pancreatic cells express transgene at day 28 ) , and allows the vector to be readministered . ^^^ Blockade of CD 40 ligand and T helper cell depletion are transient regimens that induce systemic immunosuppression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated levels of biologically active soluble CD 40 ligand in the serum of patients with chronic lymphocytic leukaemia . ^^^ Patients ' autologous plasma rescued CLL cells from spontaneous apoptosis , an effect that could be reversed with anti CD 40 ligand ( CD40L ) antibodies . ^^^ The levels of soluble CD 40 ligand in the sera of 51 CLL patients and 55 healthy donors were determined by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mice with disrupted genes for CD 40 and CD 40 ligand ( CD40L ) are unable to clear infection with Cryptosporidium parvum and develop cholangitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand inhibits Fas / CD95 mediated apoptosis of human blood derived dendritic cells . ^^^ Soluble trimeric CD 40 ligand potently inhibited both CD 95 mediated and spontaneous apoptosis in DC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand on activated platelets triggers an inflammatory reaction of endothelial cells . ^^^ CD 40 ligand ( CD40L , CD 154 ) , a transmembrane protein structurally related to the cytokine TNF alpha , was originally identified on stimulated CD4+ T cells , and later on stimulated mast cells and basophils . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , both DC subpopulations expressed IL 13 mRNA and protein following activation with PMA ionomycin , but not with CD 40 ligand , in contrast to IL 12 and IL 10 , revealing the existence of different pathways for DC activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand / CD40 stimulation regulates the production of IFN gamma from human peripheral blood mononuclear cells in an IL 12 and / or CD 28 dependent manner . ^^^ CD 40 ligand ( CD40L ) / CD40 costimulation is an important regulator of Th 1 responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cell activation induced by anti CD 3 10 anti B cell lymphoma monoclonal antibody is enhanced by pretreatment of lymphoma cells with soluble CD 40 ligand . ^^^ Agents such as soluble CD 40 ligand can enhance expression of costimulatory molecules by the malignant B cells and improve their ability to activate T cells . ^^^ We used the 5 38C13 murine lymphoma model to assess whether the combination of soluble CD 40 ligand and anti CD 3 based bispecific antibody can enhance T cell activation induced by malignant B cells more effectively than either approach alone . ^^^ Expression of CD 80 , CD 86 , and ICAM 1 on lymphoma cells was up regulated by soluble CD 40 ligand . ^^^ Syngeneic T cells were activated more extensively by lymphoma cells when the lymphoma cells were pre treated with soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon Fc epsilonRI stimulation , basophils , but not mast cells , secreted significant amounts of immunoreactive IL 4 and IL 13 and expressed detectable CD 40 ligand ( CD40L ) and a very low level of Fas ligand ( FasL ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Conversely , expression of CD 40 ligand by stimulated T cells activates APC via CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Gene transfer of CD 40 ligand induces autologous immune recognition of chronic lymphocytic leukemia B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , we found that CD 40 ligand was able to potentiate the retinoid mediated inhibition of apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is not essential for the development of cell mediated immunity and resistance to Mycobacterium tuberculosis . ^^^ It has been proposed that the induction of cellular immunity and resistance to intracellular pathogens is dependent upon CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IFN gamma is critical for long term allograft survival induced by blocking the CD 28 and CD 40 ligand T cell costimulation pathways . ^^^ We found that perioperative blockade of the CD 28 and / or CD 40 ligand T cell costimulation pathways induces long term skin and heart allograft survival in IFN gamma+ / + recipients but fails to do so in IFN gamma / mice or in wild type mice treated with IFN gamma neutralizing Ab at the time of transplantation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and appropriate cytokines induce switching to IgG , IgA , and IgE and coordinated germinal center and plasmacytoid phenotypic differentiation in a human monoclonal IgM+IgD+ B cell line . ^^^ We have identified a human monoclonal B cell line , CL 01 , that expresses surface IgM and IgD and , upon induction with CD 40 ligand , IL 4 , and IL 10 , switches to all seven downstream isotypes , showing typical DNA switch recombination preceded by germline transcription of targeted CH regions . ^^^ Our data suggest that these processes are likely regulated by a common maturation program , the activation of which may require CD 40 ligand , IL 4 , IL 10 , and IL 6 only . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , by stimulating in vitro the CD 40 molecule on the membrane of CLL cell with the CD 40 ligand the inefficient APC is turned into an efficient APC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti CD 40 ligand antibody treatment of SNF 1 mice with established nephritis : preservation of kidney function . ^^^ Prior studies have demonstrated that treatment of young , prenephritic lupus prone mice with Ab directed against CD 40 ligand ( CD40L ) prolongs survival and decreases the incidence of severe nephritis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Disruption of GCs by injection of antibody specific for CD 154 ( CD 40 ligand ) decreased the average affinity of subsequent BM AFCs , suggesting that GCs generate the precursors of high affinity BM AFCs ; inhibition of CD 154 dependent cellular interactions after the GC reaction was complete had no effect on high affinity BM AFCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Disruption of antigen induced inflammatory responses in CD 40 ligand knockout mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we generated the 5E2 mAb which targets an antigen that is induced upon CD 40 ligand ( CD40L ) activation of MM cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Strong proliferation of resting human B cells induced in vitro by a variety of physiologic signals , including anti mu , CD 40 ligand , IL 2 , IL 4 , IL 6 , IL 13 , IFN gamma , TNF alpha , anti CD 19 , and anti CD 20 , failed to induce A myb expression , suggesting that proliferation alone is not sufficient for A myb expression in the absence of induction of a true centroblast phenotype . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In a few other cases , e . g . rat and mouse growth hormone receptor , and mouse CD 40 ligand , homology modelling by ourselves and others reveals that the said sequences are part of a loop , in similarity to all RGD sequences found in proteins with established adhesion function and known 3 dimensional structure . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To understand the molecular mechanism regulating the expansion of memory B cells and the termination of expansion by differentiation into plasma cells , we investigated the effect of interleukin 2 ( IL 2 ) , IL 4 , IL 10 and CD 40 ligand ( CD40L ) on the differentiation of GC B cells in the defined culture system containing a follicular dendritic cell ( FDC ) like cell line . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of cytoplasmic , surface and soluble forms of CD 40 ligand in mouse B cells . ^^^ CD 40 and CD 40 ligand ( CD40L ) form one of most important receptor ligand pairs that dock during T B cell interactions as part of T dependent antibody responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Diminished expression of CD 40 ligand may contribute to the defective humoral immunity in patients with MHC class 2 deficiency . ^^^ We studied T and B lymphocyte function by examining the CD 40 ligand / CD40 system in three BLS patients from two unrelated families . ^^^ CD 40 ligand expression by maximally activated BLS T cells was diminished . ^^^ This abnormality may represent immunological navet rather than a general T cell defect , since expression of activation marker CD 69 and proliferative responses to PHA or anti CD 3 were normal , and BLS T cells primed and restimulated in vitro expressed normal amounts of CD 40 ligand . ^^^ BLS B cells proliferated and produced IgE if stimulated with anti CD 40 or soluble CD 40 ligand and IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and IFN gamma synergistically restore IL 12 production in HIV infected patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using class 1 , class 2 , and CD 40 ligand ( CD40L ) deficient mice , we demonstrate that neutralizing antibodies to the viral capsid proteins prevent transgene expression following readministration of rAAV vectors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Additional stimulation with CD 40 ligand induces also expression of CD 83 and upregulates CD 80 , CD 86 , and HLA DR . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Chronic lymphocytic leukemia B cells can express CD 40 ligand and demonstrate T cell type costimulatory capacity . ^^^ In this report , we demonstrate that freshly isolated B cells from a subset of CLL cases constitutively express CD 40 ligand ( CD40L , CD 154 ) , a member of the tumor necrosis factor family which is normally expressed by activated CD 4 ( + ) T cells and mediates T cell dependent B cell proliferation and antibody production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cell division number regulates IgG 1 and IgE switching of B cells following stimulation by CD 40 ligand and IL 4 . ^^^ CD 40 ligand ( CD40L ) and IL 4 are sufficient to induce resting murine B cells to divide and switch isotypes from IgM and IgD to IgG 1 and IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is not essential for induction of type 1 cytokine responses or protective immunity after primary or secondary infection with histoplasma capsulatum . ^^^ Costimulatory molecules such as CD 40 ligand ( CD40L ) have been shown to be a central regulator of type 1 responses in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Engagement of CD 40 antigen with soluble CD 40 ligand up regulates peptide transporter expression and restores endogenous processing function in Burkitt ' s lymphoma cells . ^^^ Here we show that engagement of CD 40 Ag with soluble CD 40 ligand ( CD40L ) up regulates TAP 1 and HLA class 1 expression on BL cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand exerts differential effects on the expression of 1 gamma transcripts in subclones of an IgM+ human B cell lymphoma line . ^^^ The CD 40 : CD 40 ligand ( CD40L ) interaction plays a critical role in T cell dependent isotype switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Such T cell help to B cells is mediated through up regulation of the CD 40 ligand ( CD 154 ) on the activated T cell , which binds to CD 40 inducing B cell activation , proliferation and isotype switching in conjunction with cytokines produced by the T cell . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD 154 ) stimulation of macrophages to produce HIV 1 suppressive beta chemokines . beta chemokines play an important role in the development of immunologic reactions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Because the synthesis of IgE is tightly regulated by cytokines and CD 40 ligand ( L ) interactions , CD4+ helper T cells are obvious targets , with the aim to modulate allergen induced IgE responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The current study investigates the hypothesis that CD 40 is expressed on orbital fibroblasts and represents a pathway for interaction between these fibroblasts and CD 40 ligand expressing cells , such as T lymphocytes and mast cells . ^^^ These fibroblasts become activated through triggering of CD 40 with CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we demonstrate that although nuclear NF kappaB is transcriptionally active , the Hodgkin cells fail to activate NF kappaB dependent transcription in response to CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dominant signals leading to inhibitor kappaB protein degradation mediate CD 40 ligand rescue of WEHI 231 immature B cells from receptor mediated apoptosis . ^^^ CD 40 ligand ( CD40L ) treatment prevented the drop in NF kappaB / Rel factor binding by inducing a sustained decrease in inhibitor ( 1 ) kappaB alpha and transient decrease in IkappaB beta protein levels . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rescue of WEHI 231 cells from apoptosis by costimulation with CD 40 ligand ablated the increase in p 21 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Lyn kinase deficient ( lyn / ) mice show several abnormalities such as reduced numbers of circulating B cells , hyper IgM , and low proliferative responses induced by CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The intrathymic B cells shared features of adult thymic B cells , including CD 5 expression and proliferative responses to IL 4 + IL 5 + CD 40 ligand , but not to LPS or soluble anti IgM . ^^^ Ig production was noted upon stimulation with IL 4 + IL 5 + LPS and IL 4 + IL 5 + CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human mast cells and basophils play a key role in the pathogenesis of several immunological and inflammatory disorders , not only by producing inflammatory and fibrogenic mediators , but also by directly ( CD 40 ligand ) and indirectly secreting various cytokines and chemokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 Ligand ( CD40L ) is transiently expressed on the surface of T cells and binds to CD 40 , which is expressed on the surface of B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effects of macrophage depletion and anti CD 40 ligand on transgene expression and redosing with recombinant adenovirus . ^^^ The anti CD 40 ligand antibody MR 1 , and macrophage depleting liposomes were tested for their ability as transient immunosuppressive agents to : ( 1 ) prolong transgene expression ; and ( 2 ) permit redosing after recombinant adenovirus infusion of mice . ^^^ These results suggest that both macrophage depletion and CD 40 ligand blockade inhibit immune responses to recombinant adenovirus to slow decline of transgene expression , while only CD 40 ligand blockade inhibits anti Ad antibody generation sufficiently to allow redosing to the liver . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 12 up regulates CD 40 ligand ( CD 154 ) expression on human T cells . ^^^ We here investigated the capacity of IL 12 to modify the expression on T cells of CD 40 ligand ( CD40L or CD 154 ) , a molecule transiently expressed on activated T cells and known to be of utmost importance for cognate interaction with B cells and for activation of dendritic cells and macrophages . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Enhancement of antitumor immunity by expression of CD 70 ( CD 27 ligand ) or CD 154 ( CD 40 ligand ) costimulatory molecules in tumor cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Peak IL 12 p 40 expression in both strains was reduced threefold following treatment with neutralizing anti CD 40 ligand antibody and disease worsened in C57BL / 6 mice . ^^^ Direct activation of cultured lymph node cells by anti CD 40 MAb or soluble CD 40 ligand failed to restore deficient IL 12 production by infected BALB / c mice unless recombinant IFN beta was added to culture . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TNF alpha secretion from RA SFMC was enhanced by CD 40 ligand stimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 4 mAb inhibited the CD 3 induced expression of the CD 25 and CD 69 activation markers on the T cell surface and suppressed CD 40 ligand expression , but not that of CD 25 and CD 69 , when their expression was induced by phorbol ester plus ionomycin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cycling B cells restimulated with either soluble F ( ab ' ) 2 or immobilized anti mu displayed a selective survival advantage over cycling B cells receiving no stimulus or soluble , whole anti mu by both enhancing their responsiveness to CD 40 ligand , a Th cell derived signal , and increasing the period that the cycling B cells remained responsive to this Th cell derived signal . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , PCD could be inhibited by the survival signal mediated from the binding of the CD 40 receptor to the CD 40 ligand ( CD40L ) or to the anti CD 40 mAb . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These cells do not differ either in TCR clonotype or CD 40 ligand ( CD40L ) expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast to the defective mitogenic response of T cells , B cell function , as assessed by stimulation through the CD 40 ligand in vitro remains intact . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rather , CD4+ T cells stimulated by oral immunization in Tg mice appeared to be inappropriately primed , as evidenced by a significantly reduced level of CD 40 ligand and CD 44 expression and an increased expression of CD 95 compared to those in wild type mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Switch recombination to S gamma 1 , as measured by digestion circularization PCR , is dramatically reduced in STAT 6 deficient B cells stimulated with CD 40 ligand plus IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The addition of IL 10 to soluble CD 40 ligand IL 4 stimulated PBMC or purified B cells inhibited the PLA specific and total IgE and enhanced the IgG 4 formation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) and B 7 2 ( CD 86 ) were also expressed , but to a lower extent , while B 7 1 ( CD 80 ) expression was limited . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD40 / CD40L ( CD 40 ligand ) axis regulates several interactions between T cells and B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , these CD68brightlin DC precursors acquire mature DC characteristics ( CD86+ , CD83+ , CD54bright ) upon stimulation with CD 40 ligand plus IL 3 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of mRNA encoding IL 12 as well as CD 40 ligand ( CD40L ) was detected in the tumor draining LNs but not in the spleen of tumor bearing mice after IL 12 treatment . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Glucocorticoids inhibit CD 40 ligand expression of peripheral CD4+ lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The activity was induced in splenic B lymphocytes upon activation with LPS or CD 40 ligand . ^^^ This in vitro process is thought to be relevant to in vivo class switching for two reasons : 1 ) although only one third of the Smu minisubstrate actually contains Smu sequence , all crossovers between switch regions occurred in the Smu portion ; and 2 ) treatment of B lymphocytes with IL 4 , which enriches for switching to S gamma 1 , increases the ratio of Smu S gamma 1 to Smu S gamma 3 hybrids by 16 % after LPS treatment and by 37 % after CD 40 ligand activation , implicating this S mu primed reverse transcription of switch region transcripts as a novel mechanism of regulating the specificity of isotype switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 4 ( + ) T cells tolerized ex vivo to host alloantigen by anti CD 40 ligand ( CD40L : CD 154 ) antibody lose their graft versus host disease lethality capacity but retain nominal antigen responses . ^^^ We describe an ex vivo approach in which the blockade of CD 40 ligand ( CD40L : CD 154 ) : CD 40 interactions , a pathway required for optimal T cell expansion , induces donor CD 4 ( + ) T cells to become tolerant to host alloantigens ( 2 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Pharmacokinetics / dynamics of 5c8 , a monoclonal antibody to CD 154 ( CD 40 ligand ) suppression of an immune response in monkeys . ^^^ The pharmacokinetics and pharmacodynamics ( PK / PD ) of chimeric ( Ch5c8 ) and humanized ( Hu5c8 ) 5c8 , a monoclonal antibody that binds CD 154 ( CD 40 ligand ) , thus blocking the interaction between CD 40 and CD 154 , were investigated in cynomolgus monkeys . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Since T B cell interaction is a key event to produce antibodies , we investigated this interaction through study of CD 69 , CD 40 ligand ( CD40L ) and CD 23 expression ( three very early activation antigens ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand mediated interactions are involved in the generation of memory CD 8 ( + ) cytotoxic T lymphocytes ( CTL ) but are not required for the maintenance of CTL memory following virus infection . ^^^ In this study , we investigated the involvement of CD 40 ligand ( CD40L ) mediated interactions in these processes by analyzing the memory CTL response of CD40L deficient mice following infection with lymphocytic choriomeningitis virus ( LCMV ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transient inhibition of CD 28 and CD 40 ligand interactions prolongs adenovirus mediated transgene expression in the lung and facilitates expression after secondary vector administration . ^^^ Systemic treatment at the time of primary vector administration with a monoclonal antibody ( MR 1 ) against murine CD 40 ligand , combined with recombinant murine CTLA4Ig and intratracheal coadministration of an adenovirus vector transducing the expression of murine CTLA4Ig , prolonged adenovirus transduced beta galactosidase expression in the airways for up to 28 days and resulted in persistent alveolar expression for > 90 days ( the duration of the experiment ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of CD 40 with antibodies or a soluble construct of the CD 40 ligand was shown to protect cells from apoptosis , as demonstrated by their ability to suppress the growth inhibition exerted by the anti APO 1 antibody . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Osteosarcoma cells and Ewing ' s sarcoma cells expressing CD 40 were treated with recombinant soluble CD 40 ligand to analyze CD 40 function . ^^^ Treatment with soluble CD 40 ligand increased the level of apoptotic cells and stimulated the transcription of matrix metalloproteinase 9 gene , enhancing matrix metalloproteinase 9 enzyme secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The loss of CD 28 expression is correlated with a lack of CD 40 ligand expression rendering these CD 4 T cells incapable of promoting B cell differentiation and immunoglobulin secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , we have demonstrated a reduced CD 40 and CD 40 ligand ( CD40L ) expression on PBMNC from old subjects . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 28 co stimulation stabilizes the expression of the CD 40 ligand on T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Detailed comparison of two molecular models of the human CD 40 ligand with an 10 ray structure and critical assessment of model based mutagenesis and residue mapping studies . ^^^ The human CD 40 ligand gp 39 , a tumor necrosis factor like protein , has been the subject of intense efforts to identify the receptor binding site and to analyze naturally occurring mutations that compromise gp 39 function in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Primary human splenic B cells , resting in G 0 , show a similar 5 fold increase in CstF 64 when cultured under conditions inducing proliferation ( CD 40 ligand exposure ) . ^^^ Treating resting B cells with agents triggering this switch in Ig mRNA production along with proliferation ( CD 40 ligand plus lymphokines or Staphylococcus aureus protein A ) induces no further increase in CstF 64 above that seen for proliferation alone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Western blot analyses show that CD 40 ligand ( CD40L ) induces all NF kappaB / Rel proteins , whereas LPS activates predominantly p 50 and c Rel . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The 10 linked form of hyper IgM syndrome is caused by mutations in the CD 40 ligand gene , preventing its expression on activated T cells . ^^^ The CD 40 ligand CD 40 interaction is critical for effective isotype switching and for initiating antigen specific Tf cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antitumor effect of CD 40 ligand : elicitation of local and systemic antitumor responses by IL 12 and B 7 . ^^^ The interaction between CD 40 ligand ( CD40L , CD 154 ) and its receptor CD 40 has been implicated in the establishment of cell mediated immunity as well as humoral immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Lysophosphatidylcholine upregulates CD 40 ligand expression in newly activated human CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , interaction of Langerhans cells with CD 40 ligand transfected L cells improved the viability of irradiated Langerhans cells , counteracted the inhibition of CD 86 expression , and efficiently reduced the number of apoptotic cells after a 2 d culture . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inflammatory cytokines such as interleukin 1 , tumour necrosis factor ( TNF ) and CD 40 ligand ( a cell surface homologue of TNFalpha ) can also elicit the expression by macrophages and smooth muscle cells of proteolytic enzymes that can weaken the extracellular matrix . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recently , CD 40 has also been described on mesenchymal cells , such as endothelial cells and fibroblasts , where engagement by its ligand CD 40 ligand can lead to up regulation of costimulatory and cell adhesion molecules , as well as secretion of proinflammatory cytokines . ^^^ Since airway inflammation potentially involves cell cell interactions of T cells and eosinophils ( which express CD 40 ligand ) with airway smooth muscle ( ASM ) cells , we postulated that ASM may express CD 40 and that engagement of ASM CD 40 may modulate smooth muscle cell function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A complete down regulation of CCR 1 and CCR 5 mRNA was observed only after stimulation with CD 40 ligand of DC induced to mature by LPS treatment . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The time course of cytokine production and surface expression of CD 40 ligand and CD 3 was followed at the single cell level by flow cytometry and compared with the induction of proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These include CD 2 , CD 27 , CD 40 ligand , and signaling lymphocytic activation molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Prolonged phenotypic , functional , and molecular change in group 1 Burkitt lymphoma cells on short term exposure to CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To study the prerequisites of functional interactions between malignant B cells and intermingled T cells in B cell non Hodgkin ' s lymphomas ( B NHL ) , we examined the expression of CD 40 , CD 80 and CD 86 and their ligands CD 40 ligand ( CD40L , CD 154 ) , CD 28 and CTLA 4 ( CD 152 ) using immunohistochemistry and confocal laser scanning microscopy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cell membrane associated IL 1alpha and tumor necrosis factor a , but not CD 69 , CD 40 ligand , or CD11b , were involved in the induction of MMP 1 and PGE 2 production , as shown by blockade experiments using monoclonal antibodies and cytokine antagonists . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 23 could be normally induced on IgE / B cells after culture with IL 4 or CD 40 ligand , indicating that these cells had no inherent defect in CD 23 biosynthesis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Resistance to cytotoxic chemotherapy induced by CD 40 ligand in lymphoma cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Exogenous Fas ligand had no effect on MCL cells , whereas exogenous CD 40 ligand enhanced their survival and rescued them from fludarabine induced apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Coexpression of CD 40 and CD 40 ligand in B cell lymphoma cells . ^^^ CD 40 ligand ( CD40L ) is involved in the T cell dependent regulation of B cell growth and survival and can rescue normal germinal centre B cells and several types of malignant B cells from apoptosis in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Engagement of CD 40 with CD 154 or CD 40 ligand results in the activation of target genes . ^^^ CD 40 ligand up regulates the synthesis of IL 1alpha , and blocking this cytokine with exogenous IL 1 receptor antagonist ( IL 1ra ) or with IL 1alpha neutralizing antibodies partially attenuates the induction of PGHS 2 . ^^^ In contrast , CD 40 ligand up regulation of hyaluronan synthesis is unaffected by IL 1ra . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is pivotal to efficient control of virus replication in mice infected with lymphocytic choriomeningitis virus . ^^^ CD 40 ligand ( CD40L ) is an important molecule that is known to be involved in T B collaboration and certain aspects of cell mediated immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction of CD 40 on B cells with the CD 40 ligand ( CD40L ) on preactivated CD 4 T cells is critical for the initiation of T dependent Ab responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
STAT 6 / mice were analyzed for CD 23 levels and showed reduced expression after CD 40 ligand trimer ( CD40LT ) stimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cells that chronically bind antigen due to prolonged antigen exposure , such as self reactive B cells , are induced to express CD 95 by CD 40 ligand ( CD40L ) and are subsequently eliminated by CD 95 ligand ( CD95L ) when they present antigen to CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In 1993 , five groups independently showed that this disease results from defects in the CD 40 ligand gene . ^^^ CD 40 ligand is a 39 kDa protein expressed on the surface of activated CD4+ T cells that delivers contact dependent signals to CD 40 expressing cells : B cells , monocytes , dendritic cells , epithelial cells , endothelial cells , and fibroblasts . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cognate contact dependent T B cell interactions involving CD 154 ( CD 40 ligand ) on activated T cells and CD 40 on B lymphocytes have a critical role in antibody production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand signals optimize T helper cell cytokine production : role in Th 2 development and induction of germinal centers . ^^^ This indicates that CD 40 ligand ( CD40L ) transduces a signal to T cells that is important in the process . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thymic lymphoproliferative disease after successful correction of CD 40 ligand deficiency by gene transfer in mice . ^^^ Inherited deficiency of the CD 40 ligand ( 10 linked hyper IgM syndrome ) is characterized by failure of immunoglobulin isotype switching and severe defects of cell mediated immunity . ^^^ To test the potential for gene transfer therapy to correct this disorder , we transduced murine bone marrow or thymic cells with a retroviral vector containing the cDNA for the murine CD 40 ligand ( CD40L ) and injected them into CD40L / mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We also discuss new studies that reveal how CD 40 ligand and certain other stimuli can promote chemokine expression in atherosclerosis . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and autoantigen are involved in the pathogenesis of low grade B cell lymphomas of mucosa associated lymphoid tissue . ^^^ Experimental observations suggest that antigen and CD 40 Ligand act during cognate T / B cell interaction and are crucial for germinal center B cell maturation generating marginal zone B cells . ^^^ Furthermore , CD 40 ligand expression was evaluated by immunohistochemistry and by semiquantitative RT PCR , and ligand binding to the CD 40 of tumor B cells was studied using the CD 40 system . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Analysis of CD 40 ligand gene mutations in patients with primary biliary cirrhosis . ^^^ CD 40 ligand ( CD40L ) has a crucial role in immunoglobulin class switching in B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In these cells , CD 40 engagement by CD 40 ligand induces production of endogenous TGF beta and IL 10 , expression of germline Ialpha 1 Calpha1 and Ialpha 2 Calpha2 transcripts , mature VHDJH Calpha 1 and VHDJH Calpha 2 transcripts , and IgA secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Further , 9 cryosections from MF lesions and 9 from SS lesions were stained for LFA , ICAM 1 , CD 40 , CD 40 ligand , CD 28 , CD 80 , CTLA 4 , CD 86 , FAS , FAS ligand , CLA and CD15s . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Instead , tissue biopsies with CD23+ germinal center like regions could be distinguished from those with CD 23 T cell B cell aggregates by a fourfold increase in the frequency of tissue infiltrating CD8+ T cells , a fraction of which expressed CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We therefore analyzed the effect of Flt 3 ligand ( Flt3L ) and of CD 40 ligand on the development of human DC from blood monocytes in the absence of GM CSF . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Because passive transfer of hyperimmune serum protected mice from HSV 1 infection , we hypothesized that CD 40 ligand ( CD40L ) , which induces B cell maturation , would protect mice from HSV 1 infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Guided by the principles that genetic polymorphism is likely to identify relevant genes , that any cytokine gene picked up by a virus must matter and that natural immunosuppressive activity at mucosal surfaces should be worth exploiting , we identify as particularly worthy of attention : ( 1 ) IL 10 , ( 2 ) inhibitors of IL 12 production , ( 3 ) inhibitors of CD 40 ligand expression and ( 4 ) oral and nasal tolerance . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Full expression of IL 12 p 40 required additional signals , such as tumor necrosis factor alpha or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Differential requirements for CD 28 and CD 40 ligand in the induction of experimental autoimmune myasthenia gravis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Patients with 10 linked hyper IgM syndrome [ CD 40 ligand ( CD40L ) deficiency ] are prone to infections by intracellular parasites . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 12 production by antigen presenting cells ( APC ) can be induced by the interaction between CD 40 on the APC and CD 40 ligand ( CD40L ) expressed on T cells after activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Leukocyte transfusion associated granulocyte responses in a patient with 10 linked hyper IgM syndrome . 10 linked hyper IgM syndrome ( XHIM ) is a severe congenital immunodeficiency caused by mutations in CD 154 ( CD 40 ligand , gp 39 ) , the T cell ligand for CD 40 on B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
RESULTS : CD4+ CD 28 T cell clones lacked messenger RNA for the CD 40 ligand ( CD40L ) but transcribed the perforin gene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : Highly purified SF and peripheral blood ( PB ) B cells were isolated by negative selection in a fluorescence activated cell sorter ( FACS ) and then cultured with either L cells , CD 40 ligand ( CD40L ) transfected L cells , or type B synoviocytes in the presence or absence of interleukin 2 ( IL 2 ) , IL 4 , or IL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human non germinal center B cell interleukin ( IL ) 12 production is primarily regulated by T cell signals CD 40 ligand , interferon gamma , and IL 10 : role of B cells in the maintenance of T cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , the T ( H ) cells , as well as B cells of lupus , have a regulatory defect causing markedly increased and prolonged expression of CD 40 ligand ( CD40L ) , which mediates abnormal co stimulatory signals to autoimmune B cells , sustaining the production of pathogenic autoantibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased expression of class 2 MHC molecules and CD 40 on renal tubular epithelial cells coupled with upregulation of CD 40 ligand ( CD40L ) and interleukin 2 receptor on infiltrating T cells suggest ongoing cellular immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inducing antigen specific tolerance or interfering with important interactions between T lymphocytes and other cells by blocking CD 40 ligand or decreasing the level of interleukin 10 are some of the other approaches currently under clinical investigation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BmDC cultured under various conditions ( granulocyte macrophage CSF ( GM CSF ) or GM CSF plus IL 4 alone or in combination with Flt 3 ligand , TNF alpha , LPS , or CD 40 ligand ( CD40L ) ) were analyzed morphologically , phenotypically , and functionally and were tested for their ability to promote prophylactic and / or therapeutic antitumor immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand ( CD40L ) and CD40L transfected fibroblasts stimulated HIV 1 replication in purified mature DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The depletion of macrophages in these mice resulted in a decrease in the Th 1 immune response along with an increase in the Th 2 immune response because of significant decreases in the expression of macrophage derived cytokines , particularly interleukin 12 , and a decrease in beta cell specific T cell activation , as shown by significant decreases in the expression of Fas ligand ( FasL ) , CD 40 ligand ( CD40L ) , and perforin , as compared with control mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 ligand ( CD40L ) was also increased but was largely restricted to CD 68 ( + ) macrophages . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Correction of neutropenia and hypogammaglobulinemia in 10 linked hyper IgM syndrome by allogeneic bone marrow transplantation . 10 linked hyper IgM ( 10 HIM ) syndrome is a primary immunodeficiency disease characterized by defects in both cellular and humoral immunity . 10 HIM is caused by mutations in the gene for CD 40 ligand ( CD40L ) , a T cell membrane protein that mediates T cell dependent immune functions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the presence of interleukin 2 ( IL 2 ) or supplemental recombinant CD 40 ligand plus IL 2 , Ig production by SAC induced B cells was dramatically enhanced . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Consistent with the decrease in IFN gamma production , splenocytes from ICSBP / mice stimulated with anti CD 3 in the presence or absence of IFN gamma or a soluble CD 40 ligand agonist failed to produce IL 12 p 40 and IL 12 p 70 protein ; however , the deficient production of IFN gamma from ICSBP / mice could be restored by the addition of anti CD 28 Ab in an IL 12 independent manner . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Splenic T cells from SPF NC / Nga mice had a level of CD 40 ligand ( CD40L ) expression comparable to that of BALB / c mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TWHf also inhibited both phorbol 12 myristate 13 acetate induced IL 2Ralpha expression and ionomycin induced CD 40 ligand expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DC were generated in vitro using granulocyte macrophage colony stimulating factor +interleukin 4 in combination with either tumor necrosis factor alpha or CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand expression deficiency in a female carrier of the 10 linked hyper IgM syndrome as a result of 10 chromosome lyonization . ^^^ Since this patient is the sister of a boy affected with a hyper IgM syndrome due to a defect in CD 40 ligand ( CD40L ) expression , the involvement of CD40L in this phenotypic expression was investigated . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human Th 1 responses driven by IL 12 are associated with enhanced expression of CD 40 ligand . ^^^ CD 40 ligand ( CD40L ) plays important roles in regulation of immune responses , including T cell dependent activation of B cells and cytokine production by monocytes and dendritic cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the present study we examined the expression of CD 40 , CD 40 ligand ( CD40L ) , CD 80 , CD 86 , CD 28 and cytolytic T lymphocyte associated antigen 4 ( CTLA 4 ) on lymphocytes immunohistochemically . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These data highlight the mechanisms of IL 12 production by human monocytes exposed to C . neoformans , indicating a possible biphasic secretion of IL 12 , dependent on the direct effect of fungal insult , and characterized by consistent secretion of IL 12 that is dependent on the interaction of CD 40 with the CD 40 ligand expressed on activated T cells responding to C . neoformans . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mucosally induced systemic T cell unresponsiveness to ovalbumin requires CD 40 ligand CD 40 interactions . ^^^ CD 40 ligand ( CD40L ) gene disrupted ( CD40L / ) mice were employed to examine the role of costimulatory signals via CD40L CD 40 interactions in mucosally induced tolerance . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Whereas , previously reported , B cells lacking p 50 alone had a severe proliferative defect in response to LPS , a moderate defect in response to CD 40 ligand ( CD40L ) , and normal proliferation to Ag receptor cross linking using dextran conjugated anti IgD Abs ( alpha delta dex ) , B cells lacking both p 50 and p 65 exhibited severely impaired proliferation in response to LPS , alpha delta dex , and CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( L ) , FasL , and TNF alpha are members of the TNF family of cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
H1R deficient T and B cells showed normal responses upon stimulation with interleukin ( IL ) 2 , IL 4 , CD 40 ligand , CD 40 ligand plus IL 4 , and lipopolysaccharide . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 receptor was activated by incubating NPC cells with murine L cells producing CD 154 , the CD 40 ligand . ^^^ These data suggest that NPC tumor infiltrating lymphocytes , which often produce the CD 40 ligand in situ , may increase the survival of malignant cells , thereby enhancing tumor growth in patients . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , T cell derived as well as recombinant CD 40 ligand ( CD40L , CD 154 ) , an inflammatory mediator recently localized in atheroma , induced de novo synthesis of stromelysin 3 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Eight different CD 40 mAb shared with soluble trimeric CD 40 ligand ( sCD40LT ) the capacity to rescue germinal center ( GC ) B cells from spontaneous apoptosis and to suppress antigen receptor driven apoptosis in group 1 Burkitt ' s lymphoma cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Hence , these pre activated cells undergo vigorous proliferation when stimulated with either optimal or suboptimal concentrations of weakly mitogenic anti CD 40 mAb , or with soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Moreover , TRANCE cooperates with CD 40 ligand or TNF alpha to further increase the viability of DCs , suggesting that several TNF related molecules on activated T cells may cooperatively regulate the function and survival of DCs to enhance T cell mediated immune responses . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
LAG 3 is expressed at high levels on human T cells cocultured with autologous monocytes and IL 2 and synergizes with the low levels of CD 40 ligand ( CD40L ) expressed on these cells to trigger TNF alpha and IL 12 production by monocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The TH 2 cytokine IL 4 enhanced DC 1 maturation and killed pDC 2 , an effect potentiated by IL 10 but blocked by CD 40 ligand and interferon gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
S MBP specific T cells to upregulate CD 40 ligand expression and to induce the production of IL 12 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Further investigation of the molecular mechanism demonstrated that tetrandrine inhibited the expression of the protein kinase C dependent interleukin 2 receptor alpha chain and CD 69 but not the expression of the Ca2+ dependent CD 40 ligand and CD 69 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro culture alone and crosslinking with the CD 40 ligand up regulated DC CD80 / CD86 expression and costimulator function , and this was not affected by inclusion of SF . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
At the same time the expression of IL 1 , IL 2 , IL 3 , IL 6 , CD 40 ligand and IL 2R genes was not detected . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DC at the early stages of maturation ( 2 and 4 h ) produced elevated amounts of IL 12 p 70 during interaction with CD 40 ligand bearing Th cells or , after stimulation with the T cell replacing factors , soluble CD 40 ligand and IFN gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These cells are surface IgM+ IgD+ and , in the absence of T cells , switch to IgG , IgA , and IgE in response to CD 40 : CD 40 ligand engagement and exposure to appropriate cytokines . ^^^ In these cells , induction of somatic mutations required cross linking of the surface receptor for Ag and T cell contact through CD 40 : CD 40 ligand and CD 80 : CD 28 coengagement . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Differential effects of CD 40 ligand / trimer stimulation on the ability of dendritic cells to replicate and transmit HIV infection : evidence for CC chemokine dependent and independent mechanisms . ^^^ The role of exogenous stimulation of CD 40 by CD 40 ligand ( CD40L ) in dendritic cell ( DC ) maturation , CC chemokine production , and CCR 5 receptor expression was examined using a soluble trimeric CD40L agonist protein ( CD40LT ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TRANCE , a tumor necrosis factor family member critical for CD 40 ligand independent T helper cell activation . ^^^ CD 40 ligand ( CD40L ) , a tumor necrosis factor ( TNF ) family member , plays a critical role in antigen specific T cell responses in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand mutants responsible for 10 linked hyper IgM syndrome associate with wild type CD 40 ligand . ^^^ CD 40 ligand ( CD40L ) is a 33 kDa type 2 membrane glycoprotein mainly expressed on activated CD 4 ( + ) T cells in trimeric form . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This genomic organization is similar to that of other members of the TNF family , especially the CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of CD 154 ( CD 40 ligand ) mRNA stability during T cell activation . ^^^ The CD 154 protein ( CD 40 ligand ) , which is critical to the regulation of both humoral and cellular immune responses , is expressed transiently on the surface of activated CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand CD 40 interaction induces chemokines in cervical carcinoma cells in synergism with IFN gamma . ^^^ In this study , we show high CD 40 expression on cervical carcinoma cells and CD 40 ligand ( CD40L ) staining on attracted T cells in tumor tissue , suggesting a paracrine stimulation mechanism via CD40L CD 40 interactions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In sharp contrast , CD 40 ligand cognate signal induces full maturation of LC and is not inhibited by TGF beta 1 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Defects of T cell effector function and post thymic maturation in 10 linked hyper IgM syndrome . 10 linked hyper IgM syndrome ( XHIM ) results from mutations in the gene encoding for CD 40 ligand ( CD 154 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The inhibitors suppressed CD 40 ligand ( CD40L ) expression on activated CD 4 T cells , which may explain this Th 2 differentiating effect . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of human breast carcinoma growth by a soluble recombinant human CD 40 ligand . ^^^ Therefore , we wanted to assess the effects of a recombinant soluble human CD 40 ligand ( srhCD40L ) on human breast carcinoma cell lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Taken together , this data demonstrates that in vitro expression of costimulatory molecules such as CD 80 , CD 86 , CD 27 and CD 70 , which are implicated in the anti tumoral response , can be regulated by CD 40 ligand but not by TNF . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The evolutionarily conserved sequence upstream of the human Ig heavy chain S gamma 3 region is an inducible promoter : synergistic activation by CD 40 ligand and IL 4 via cooperative NF kappa B and STAT 6 binding sites . ^^^ Reporter luciferase gene vectors containing the human gamma 3 ECS were used to transfect CL 01 cells , which have been shown to undergo Smu > S gamma 3 DNA recombination , upon engagement of CD 40 by CD 40 ligand ( CD40L ) and exposure to IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Moreover , this prevention in down modulation is independent of CD 40 : CD 40 ligand contact . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NK cells from CD 40 ligand ( CD40L ) / or CD 28 / mice were triggered by tumor cells transfected with CD 40 and B 7 2 , respectively , and these transfectants were rapidly eliminated in vivo when inoculated into CD40L / and CD 28 / mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated levels and functional capacity of soluble CD 40 ligand in systemic lupus erythematosus sera . ^^^ OBJECTIVE : To measure soluble CD 40 ligand ( sCD40L ) in sera from patients with systemic lupus erythematosus ( SLE ) and to study the functional capacity of sCD40L in mediating B cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , the severely anergic state of anti dsDNA B cells is partially reversible upon stimulation with CD 40 ligand and IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : The expression of CD 40 ligand ( CD40L ) in synovial tissues ( ST ) from patients with RA was examined by immunohistochemistry . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of the CD 40 ligand ( CD40L , CD 154 ) by activated T cells from the recipient remained at low levels until 10 months after the transplantation , but then normalized . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , increased release of sTNF R 75 into serum 8 days post LCMV infection was mediated via T cells but independently of both CD 40 ligand and IFN gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand or monocyte conditioned medium induced maturation of HIV 1Ba L infected DC reduced virus production by about 1 Log , while cells became CCR 5 ( ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : The expression of CD 40 and CD 40 ligand ( CD40L ) in mononuclear cells ( MNCs ) infiltrating the salivary glands of patients with Sjgren ' s syndrome ( SS ) has recently been reported . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Responsiveness of peripheral blood B cells to recombinant CD 40 ligand in patients with systemic lupus erythematosus . ^^^ Recombinant CD 40 ligand leucine zipper fusion protein ( CD40L LZ ) significantly enhanced 3H TdR incorporation by both SLE and normal B cells ( P < 0 . 01 ) . 3H TdR incorporation of SLE B cells without stimuli ( P < 0 . 001 ) and with CD40L LZ stimulation ( P < 0 . 05 ) were significantly lower in SLE patients compared with normal controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the APC Th cell interaction , p 40 mRNA accumulation in APC was shown to be up regulated by stimulation with CD 40 ligand ( CD40L ) on Th cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After exposure to TNF alpha or CD 40 ligand , Dex DC expressed lower levels of CD 83 and CD 86 than untreated cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD 40 ligand interaction is central to cell mediated immunity against Toxoplasma gondii : patients with hyper IgM syndrome have a defective type 1 immune response that can be restored by soluble CD 40 ligand trimer . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Production of IL 4 and expression of CD 40 ligand by human CD 8 T cells . ^^^ OBJECTIVE : The aim of this study was to investigate IL 4 production and CD 40 ligand expression by human CD 8 ( + ) T cells . ^^^ We also examined the expression of IL 4 and CD 40 ligand by CD 8 ( + ) T cells from a patient with adenosine deaminase deficiency who received autologous T cell directed gene therapy . ^^^ RESULTS : Transfer of atopic cells into the mice caused production of IgE and IgG with increased expression of IL 4 and CD 40 ligand mRNA . ^^^ In addition , both intracellular IL 4 and cell surface CD 40 ligand were detected in CD 8 ( + ) and in CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Compared with BLC stimulated CTL , EC stimulated CTL clones often fail to secrete IFN gamma after target cell contact ( p = 0 . 0006 ) and constitutively express CD 40 ligand ( CD40L ) at rest ( p = 0 . 0006 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The generation of marginal zone B cells and their localization in the marginal sinus area are T cell and CD 40 ligand independent , suggesting that they do not represent a postgerminal center population . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After stimulation with LPS , TNF alpha or CD 40 ligand , the inflammatory chemokines MIP 1alpha , MIP 1beta and IL 8 were produced rapidly and at high levels , but only for a few hours , while RANTES and MCP 1 were produced in a sustained fashion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human B cells accumulate immunoglobulin 5 gene somatic mutations in a cell contact dependent manner in cultures supported by activated T cells but not in cultures supported by CD 40 ligand . ^^^ To dissect the role of different components of the germinal centre in this mechanism , we have used in vitro cultures of normal human IgD+ peripheral blood B lymphocytes co cultured with activated CD4+ T cells , or with resting CD4+ T cells , or with CD 40 ligand and IL 4 . ^^^ In contrast , in the absence of activated T cells , transcripts contained only background levels of somatic mutation irrespective of the presence of resting T cells or CD 40 ligand and IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti tumor immunity induced by in vivo adenovirus vector mediated expression of CD 40 ligand in tumor cells . ^^^ CD 40 ligand ( CD40L ) , the ligand for CD 40 on antigen presenting cells , is essential for the initiation of antigen specific T cell responses , an important component of the immune response to tumors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Because HIM results from defects in either T cell CD 154 ( CD 40 ligand ) expression or abnormal CD 40 signaling , the molecular basis of the patient ' s disease was analyzed . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of the germline immunoglobulin Cgamma 1 promoter by CD 40 ligand and IL 4 : dual role for tandem NF kappaB binding sites . ^^^ In the present study , we investigated the role of three tandem NF kappaB sites ( site 1 , 95 ; site 2 , 71 ; site 3 , 53 ) in the regulation of the germline Cgamma 1 Ig promoter by CD 40 Ligand ( CD40L ) and IL 4 in the mouse B lymphoma cell line , BCL 1 3B3 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This nontoxic stem cell transplantation can be applied to allogeneic transplant with the addition of a tolerizing step ; in this case presensitization with donor spleen cells and administration of CD 40 ligand antibody to block costimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We examined expression of the CD 40 ligand ( CD40L ) on the patient ' s activated T lymphocytes and in vitro production of immunoglobulins by his lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dendritic cells are highly effective at inducing an immune response to antigens to which the host is unresponsive , while transgenic expression of the costimulator molecule CD 40 ligand ( gp39 / CD154 ) and the T cell growth factor interleukin 2 ( IL 2 ) are also able to augment immune responsiveness . ^^^ In additional mice , the pulsed DC were mixed with genetically modified syngeneic fibroblasts that were expressing CD 40 ligand or secreting interleukin 2 ( IL 2 ) . ^^^ This suppression was further enhanced when pulsed DC were coinjected with fibroblasts expressing CD 40 ligand and IL 2 ( P < 0 . 0005 compared to DC alone ) even though CD 40 ligand and IL 2 expressing fibroblasts alone offered no significant protection in this model . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Although the virus alone did not stimulate proliferation of purified B cells in vitro , it was co stimulatory with CD 154 ( CD 40 ligand ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Role of CD 40 ligand in Mycobacterium avium infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of cytotoxic T lymphocyte ( CTL ) responses against minor histocompatibility antigens is dependent upon the presence of T cell help and requires the interaction of CD 40 on dendritic cells ( DCs ) with CD 40 ligand on activated T helper cells ( Th ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cells were isolated from lymphoid follicles of sheep Peyer ' s patches ( PP ) and co cultured with murine CD 40 ligand ( mCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Incubation with a membrane associated form of CD 40 ligand resulted in the up regulation of costimulatory molecules on MHC class 2 / DC and fully rescued their ability to induce antitumor immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study demonstrates that Ag activated CD4+ NKT cells express CD 40 ligand ( CD40L ) ( CD 154 ) , which engages CD 40 on APC and stimulates them to produce IL 12 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The latter effect was associated with increased T cell CD 40 ligand expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Differential induction of cytokine genes and activation of mitogen activated protein kinase family by soluble CD 40 ligand and TNF in a human follicular dendritic cell line . ^^^ Both the soluble form of CD 40 ligand ( sCD40L ) and TNF increased the level of M CSF and G CSF mRNA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vivo Ig responses to soluble , haptenated polysaccharide ( PS ) Ags are T cell independent and do not require CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction of T lymphocytes expressing the CD 40 ligand ( CD 154 ) and cells of the vessel wall expressing the corresponding receptor protein ( CD 40 ) may play an important role in chronic inflammation including arteriosclerosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We studied this regulation in more detail by combined treatment with CD 40 ligand and the proinflammatory cytokine interleukin 1 ( IL 1 ) in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cutting edge : CD 40 ligand is a limiting factor in the humoral response to T cell dependent antigens . ^^^ CD 40 ligand ( CD40L ) plays a crucial role in T cell dependent B cell responses , but whether its abundance is a limiting factor in their development is unclear . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Synergistic action of fms like tyrosine kinase 3 ligand and CD 40 ligand in the induction of dendritic cells and generation of antitumor immunity in vivo . ^^^ Here , we show that Flt3L and CD 40 ligand ( CD40L ) synergize in the generation of immune responses against two poorly immunogenic tumors , leading to complete tumor rejection in a high proportion of mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After stimulation with recombinant CD 40 ligand ( CD40LT ) , cytokine and beta chemokine production were similar by DC from HIV donors infected in vitro with the CCR 5 using HIV Ba L strain ( n = 8 ) compared with uninfected DC from the same donors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro system for immunoglobulin class switching using BHK cells transfected with murine recombinant CD 40 ligand . ^^^ To develop an in vitro system for mouse immunoglobulin ( Ig ) class switching , the expression vector of murine CD 40 ligand ( CD40L ) which is expressed on T cells was transfected to BHK cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 , a cell surface molecule found on B lymphocytes and other antigen presenting cells , can , when engaged by CD 40 ligand ( CD40L ) , induce gene rearrangements and isotype switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
For protracted treatment , we showed that readministration of recombinant adenovirus vectors could be facilitated by transient immunosuppression using a monoclonal antibody against CD 40 ligand ( MR 1 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dendritic cells infiltrating tumors cotransduced with granulocyte / macrophage colony stimulating factor ( GM CSF ) and CD 40 ligand genes take up and present endogenous tumor associated antigens , and prime naive mice for a cytotoxic T lymphocyte response . ^^^ We transduced BALB / c derived C 26 colon carcinoma cells with granulocyte / macrophage colony stimulating factor ( GM CSF ) and CD 40 ligand ( CD40L ) genes to favor interaction of these cells with host dendritic cells ( DCs ) and , therefore , cross priming . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Epitope dependent synergism and antagonism between CD 40 antibodies and soluble CD 40 ligand for the regulation of CD 23 expression and IgE synthesis in human B cells . ^^^ The natural counterstructure to CD 40 is the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , DCIR expression was down regulated by signals inducing DC maturation such as CD 40 ligand , LPS , or TNF alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand activated human monocytes amplify glomerular inflammatory responses through soluble and cell to cell contact dependent mechanisms . ^^^ Human peripheral blood monocytes , primed with IFN gamma and GM CSF , were activated with CD 40 ligand ( CD40L ) or TNF alpha and cocultured with MC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Enhanced levels of soluble and membrane bound CD 40 ligand in patients with unstable angina . ^^^ BACKGROUND : The CD 40 ligand ( CD40L ) on activated T cells and platelets may be activating matrix metalloproteinases , inducing procoagulant activity , and be involved in the pathogenesis of acute coronary syndromes by promoting plaque rupture in atheroma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Allergen stimulated expression of CD 154 ( CD 40 ligand ) on CD3+ lymphocytes in atopic , but not in nonatopic individuals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Decreased CD 40 ligand induction in CD 4 T cells and dysregulated IL 12 production during HIV infection . ^^^ Two factors are essential in this process : priming APC with IFN gamma and triggering the CD 40 receptor on APC by CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this paper we demonstrate that cell membrane expressed CD 154 ( CD 40 ligand ) is able to support the continual growth of porcine mesenteric lymph node B cell cultures for more than 4 months without the addition of exogenous cytokines , such as interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) did not participate in such a phenomenon . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functional CD 40 ligand is expressed on epidermal Langerhans cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human endothelial cells augment early CD 40 ligand expression in activated CD4+ T cells through LFA 3 mediated stabilization of mRNA . ^^^ Human endothelial cells ( EC ) augment CD 40 ligand ( CD40L ) expression on PHA activated CD4+ T cells at early times ( e . g . , 4 6 h ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After maturation with tumor necrosis factor alpha or CD 40 ligand in Dex treated DC , despite a reduction induced by maturation the endocytic activity of FITC dextran and LY , the expression of MR , CD 16 and CD 32 remained higher than in control DC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we report that apoptosis prevails over stimulation when a BCR agonist is applied to human memory B cells which have been preactivated by CD 40 ligand or anti immunoglobulin antibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligation by trimeric CD 40 ligand ( CD40L ) resulted in a 20 45 % inhibition of tumor cell growth in three of seven cell lines tested . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Neutralizing Abs against CD 40 ligand ( CD40L ) , but not against IL 4 or IL 15 , abrogated IgM RF production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Variations in the method of surface activation , with particular emphasis on the CD 40 receptor , reveals that immobilized CD 40 ligand may be sufficient , in and of itself , to up regulate IL 13Ralpha1 , which may bear significance for B lymphocyte bystander proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mutations of the CD 40 ligand gene and its effect on CD 40 ligand expression in patients with 10 linked hyper IgM syndrome . 10 linked hyper IgM syndrome ( XHIM ) is a primary immunodeficiency disorder caused by mutations of the gene encoding CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Hyperexpression of CD 40 ligand ( CD 154 ) in inflammatory bowel disease and its contribution to pathogenic cytokine production . ^^^ CD 40 ligand ( CD40L or CD 154 ) , a type 2 membrane protein with homology to TNF , is transiently expressed on activated T cells and known to be important for B cell Ig production and for activation and differentiation of monocytes and dendritic cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Second , we propose that T cell signals , both secreted ( e . g . , cytokines ) and contact dependent ( e . g . , CD 40 ligand ) , activate new gene expression in EC that induce the capacity to perform new effector functions , such as leukocyte recruitment and activation or initiation of intravascular coagulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Treatment of resting murine B lymphocytes with CD 40 ligand ( CD40L ) and IL 4 induces proliferation and a switch in immunoglobulin ( Ig ) isotype surface expression from IgM and IgD to IgG 1 and IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The soluble CD 40 ligand sCD 154 in systemic lupus erythematosus . ^^^ The ability of SLE plasma to induce such phenotypic changes could be abrogated by neutralizing antibodies specific for the CD 40 ligand ( CD 154 ) but not by antibodies to TNF alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon CD 40 ligand stimulation of lyn / B cells , 10 fold less IL 4 was required to induce switching from IgM to IgG 1 and IgE and an increased proportion of B cells isotype switched at each IL 4 concentration . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Characterization of RNA binding proteins associated with CD 40 ligand ( CD 154 ) mRNA turnover in human T lymphocytes . ^^^ CD 154 ( CD 40 ligand ( CD40L ) ) has been demonstrated to play an essential role in the development of humoral and cellular immunity through its interaction with CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 28 in vivo induces CD 40 ligand expression and promotes B cell survival . ^^^ Among these accessory molecules , interactions between CD28 / CTLA 4 with B 7 family members ( CD 80 and CD 86 ) and CD 40 with CD 40 ligand ( CD40L ) play a decisive role in regulating the progression of balanced immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated CD 154 ( CD 40 ligand ) synthesis in T cells from allergic patients after nonspecific stimulation in vitro . ^^^ The interaction of the CD 154 molecule ( CD 40 ligand , gp 39 ) on activated T cells with the CD 40 antigen on B cells seems to play a key role in immunoglobulin class switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Requirement for CD 40 ligand , CD 4 ( + ) T cells , and B cells in an infectious mononucleosis like syndrome . ^^^ Aspects of the CD 8 ( + ) T cell response are substantially modified in mice that lack B cells , CD 4 ( + ) T cells , or the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After allergen activation , CD 40 ligand was upregulated on a subset of peripheral blood basophils . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Bryostatin and CD 40 ligand enhance apoptosis resistance and induce expression of cell survival genes in B cell chronic lymphocytic leukaemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This treatment primed the cells for further stimulation with anti CD 40 monoclonal antibody ( MoAb ) presented on irradiated CD32L cells ( the CD 40 system ) or soluble CD 40 Ligand , and a combination of Trx and cytokines ( IL 4 + IL 10 ) , which allowed the cells to be maintained for up to 1 month with preserved viability and a variable rate of proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro experiments supported these results and showed that IL 1beta , TNF alpha , and CD 40 ligand potentiated IP 10 expression from IFN gamma stimulated ECs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The mode of activation altered the relationship with division , as CD 40 ligand increased switching to IgA and decreased switching to IgG2b and IgG 3 when measured per division . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand blockade induces CD4+ T cell tolerance and linked suppression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It has been shown , for example , that down modulation of CD 154 ( CD 40 ligand ) , which allows B cells to respond to T cells , may account for some of the immune defects of these cells in patients with chronic lymphocytic leukemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It was recently shown that one immunological abnormality found in male BXSB mice encompasses B cell expression of CD 40 ligand ( CD40L ) by an expanded population of large B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Monoclonal antibodies ( mAb ) to CD 40 ligand ( CD40L ) and human leucocyte antigen ( HLA ) DR , but not lymphocyte function associated antigen 1 ( LFA 1 ) , LFA 3 or HLA class 1 , significantly inhibited the T lymphocyte induction of DC costimulator expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD 40 ligand ( CD40L ) in CD 8 T cell activation was assessed by tracking antigen specific T cells in vivo using both adoptive transfer of T cell receptor transgenic T cells and major histocompatibility complex ( MHC ) class 1 tetramers . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent progress in DC biology has demonstrated that DCs can be activated via a variety of substances , e . g . various cytokines , CD 40 ligand , bacterial products , and haptens , to increase their antigen presenting ability , probably by different mechanisms . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of human B cells with recombinant NAIL in the presence of a suboptimal concentration of human CD 40 ligand or IL 4 resulted in increased proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , COX 2 and the prostanoids PGE ( 2 ) , PGF ( 2alpha ) and PGD ( 2 ) are highly inducible in B / macrophage cells upon stimulation with lipopolysaccharide , CD 40 ligand , or via engagement of surface IgM , supporting a role for these cells in inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 154 variants associated with hyper IgM syndrome can form oligomers and trigger CD 40 mediated signals . 10 linked hyper IgM syndrome is a rare immunodeficiency disorder resulting from mutations in the gene encoding the CD 40 ligand ( CD 154 ) molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blocking antibody to CD 40 and CD 154 ( CD 40 ligand ) decreased the ability of monocytes to aid in T cell survival , whereas , blocking LFA 1 / I CAM 1 , Fas ligand and the CD4 / major histocompatibility complex class 2 interaction did not affect the influence of monocytes on T cell survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
No alteration in T lymphocyte expression of CD 40 ligand ( CD 154 ) in individuals with or at increased risk for insulin dependent diabetes mellitus . ^^^ CD 40 ligand ( CD40L ) regulates multiple phases of the humoral and cellular immune response through binding to CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 12 reverses the suppressive effect of the CD 40 ligand blockade on experimental autoimmune encephalomyelitis ( EAE ) . ^^^ Blockade of the CD 40 ligand ( CD40L ) CD 40 interaction suppresses experimental autoimmune encephalomyelitis ( EAE ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Neutralizing mAbs to CD 40 ligand had no effect on the cytokine levels produced by asthmatic tissue or PBMCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunomodulatory effect of a plasmid expressing CD 40 ligand on DNA vaccination against human immunodeficiency virus type 1 . ^^^ CD 40 ligand is a costimulatory molecule which acts a potent immunomodulator . ^^^ We found the mice inoculated with human CD 40 ligand expression plasmid ( pMEhCD40L ) combined with human immunodeficiency virus type 1 ( HIV 1 ) DNA vaccine exhibited both humoral and cellular antigen specific immunological enhancement . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of DC maturation by LPS , monocyte conditioned media ( MCM ) , or stimulation through CD 40 ligand ( CD40L ) rendered the DC less susceptible to lysis by NK cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The rescue of WEHI 231 cells by CD 40 ligand engagement of its receptor prevented the increase in p 27 induction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 by CD 40 ligand on these cells results in microglial activation , as measured by TNF alpha production and neuronal injury . ^^^ Furthermore , cotreatment of microglial cells with CD 40 ligand and TGF beta 1 or IL 10 , or both , inhibits CD 40 mediated activation of p44 / 42 MAPK and production of TNF alpha in a statistically interactive manner . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 4 ( + ) T cell mediated protection against a lethal outcome of systemic infection with vesicular stomatitis virus requires CD 40 ligand expression , but not IFN gamma or IL 4 . ^^^ In contrast , mice deficient in expression of CD 40 ligand were more susceptible than wild type mice , and residual resistance in these mice was almost completely abrogated by depletion of CD 8 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant human CD 40 ligand inhibits simian immunodeficiency virus replication : a role for interleukin 16 . ^^^ CD 40 ligand ( CD40L ) , expressed on activated T cells , binds its receptor , CD 40 , on dendritic cells , B cells , and monocytes / macrophages . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Both the expression of activation markers ( CD 25 , CD 29 , CD 40 ligand , CD 69 and HLA DR ) on freshly isolated PBL and T cell subsets , and the expression of intracellular cytokines ( IL 4 , IL 10 , interferon gamma ( IFN gamma ) and tumour necrosis factor alpha ( TNF alpha ) ) on mitogen stimulated PBL and T cell subsets were analysed by double immunofluorescent staining and flow cytometry . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased tumor necrosis factor alpha production and induction of neuronal injury occurred when Abeta stimulated microglia were treated with CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , SM degradation was observed in acid sphingomyelinase deficient cells , as well as after ligation by soluble CD 40 ligand . ^^^ The anti CD 40 antibody , as well as the soluble CD 40 ligand induced a decrease in thymidine incorporation and morphological features of apoptosis , which were mimicked by cell permeant or bacterial sphingomyelinase produced ceramides . ^^^ Stable expression of a dominant negative form of the FAN protein ( factor associated with neutral sphingomyelinase activation ) , which has been reported to mediate tumor necrosis factor induced activation of neutral sphingomyelinase , significantly inhibited CD 40 ligand induced sphingomyelinase stimulation and apoptosis of transformed human fibroblasts . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In agreement with their activated phenotype , phab 5 3 ( + ) B cells display a blast like morphology , and are prone to spontaneous apoptosis in vitro , unless rescued by stimulation with CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
L 3055 cells proliferated continuously in the presence of HK cells , while they differentiated into a population with the phenotype of centrocytes after stimulation with CD 40 ligand ( CD40L ) and IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Tc2 / 0 clones expressed CD 30 and CD 40 ligand at a much higher level than Tc 1 clones . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , f spleen cells produced IL 12 upon CD 40 ligand stimulation , and the production was hardly affected by the presence of IFN gamma or preincubation . ^^^ These results indicate that IFN gamma priming is not critical for IL 12 production of spleen cells stimulated with LPS or CD 40 ligand , although IFN gamma enhances the production , especially , in response to LPS stimulation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In cultured vascular SMC , ligation of CD 40 with native CD 40 ligand ( CD40L ) derived from activated T lymphocytes or recombinant human CD40L ( rCD40L ) induced the transient expression of TF on the cell surface ( as determined by FACS analysis ) in a concentration and time dependent manner and enhanced total cell associated TF ( as determined by ELISA ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Analysis of the requirements for the induction of CD4+ T cell alloantigen hyporesponsiveness by ex vivo anti CD 40 ligand antibody . ^^^ Recently , we described an ex vivo approach in which the blockade of the CD 40 ligand ( CD40L ) : CD 40 costimulatory pathway in bulk MLR cultures induces donor CD4+ T cells to become specifically tolerant to MHC class 2 disparate alloantigenic bearing stimulators , resulting in a profound reduction in GVHD generation in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Resting B lymphocytes as APC for naive T lymphocytes : dependence on CD 40 ligand / CD40 . ^^^ In the absence of an activating signal through the B cell Ag receptor , T cell proliferation after Ag recognition on resting B cells depends on CD 40 expression on the B cells , implying that naive T cells use the membrane bound cytokine , CD 40 ligand ( CD 154 ) , to induce the costimulatory signals that they need . ^^^ Induction of B 7 1 ( CD 80 ) and increased or sustained expression of CD44H , ICAM 1 ( CD 54 ) , and B 7 2 ( CD 86 ) are dependent on the interaction of CD 40 ligand with CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A series of observations strongly suggests that ceramide involved in stress induced apoptosis is not endolysosomal : 1 ) Although short chain ceramides induced apoptosis , loading cells with natural ceramide through receptor mediated endocytosis did not result in cell death . 2 ) Neither TNF alpha nor anti CD 95 induced the degradation to ceramide of a natural SM that had been first introduced selectively into acidic compartments . 3 ) Stimulation of SV 40 transformed fibroblasts by TNF alpha or CD 40 ligand resulted in apoptosis equally well in cells derived from control individuals and from patients affected with Farber disease , having a genetic defect of acid ceramidase activity leading to lysosomal accumulation of ceramide . ^^^ In all cases , apoptosis was preceded by a comparable increase of intracellular ceramide levels . 4 ) Retroviral mediated gene transfer and overexpression of acid ceramidase in Farber fibroblasts , which led to complete metabolic correction of the ceramide catabolic defect , did not affect the cell response to TNF alpha and CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expression of CD 28 , CTLA 4 , CD 40 ligand ( CD40L ) on CD4+ T cells was analysed by flow cytometry . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 and CD 40 ligand in the human conjunctival epithelium . ^^^ The expressions of CD 40 and CD 40 ligand ( CD40L ) were investigated ex vivo and in vitro in conjunctival epithelial cells , in correlation with HLA DR class H antigen , previously shown to be upregulated in conjunctival inflammatory conditions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study also demonstrates that anti dsDNA B cells , regardless of maturation status or follicular localization , are able to proliferate and up regulate the costimulatory molecule B 7 2 in response to CD 40 ligand and IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CpG ODN induced proliferation in both B CLL cells and normal B cells ; however , only B CLL cells increased proliferative responses when CpG ODN was added to co cultures of CD 40 ligand transfected mouse fibroblasts ( CD40LF ) and B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A polymorphic CD 40 ligand ( CD 154 ) molecule mediates CD 40 dependent signalling but interferes with the ability of soluble CD 40 to functionally block CD 154 : CD 40 interactions . ^^^ We report the characterization of a naturally occurring polymorphism in CD 40 ligand ( CD40L , CD 154 ) expressed by activated T cells from a young female patient . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thromboembolic complications after treatment with monoclonal antibody against CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Measles virus induces abnormal differentiation of CD 40 ligand activated human dendritic cells . ^^^ CD 40 ligand ( CD40L ) expressed on activated T cells is shown to induce terminal differentiation of DCs into mature effector DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , regardless of CD 47 mAb treatment during DC maturation , mature DC restimulated by soluble CD 40 ligand and IFN gamma , to mimic DC / T interaction , produce less IL 12 and more IL 18 than iDCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Based on observations that in some CLL cases , the tumor cells express both CD 40 and its ligand , CD 154 ( CD 40 ligand ) , we proposed a model for CLL pathogenesis due to CD 40 ligation within the tumor . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We studied the innate and adaptive immune system of rhesus macaques infected with the virulent simian immunodeficiency virus isolate SIVmac 251 by evaluating natural killer ( NK ) cell activity , cytokine levels in plasma , humoral and virological parameters , and changes in the activation markers CD 25 ( interleukin 2R IL 2R alpha chain ) , CD 69 ( early activation marker ) , and CD 154 ( CD 40 ligand ) in lymphoid cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD 40 ligand . ^^^ In addition , the CD 40 ligand ( CD 40 L / CD154 ) , a member of the TNF family , is also expressed more widely than activated CD4+ T cells only . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recently , it has been shown that a dominant pathway of CD 4 ( + ) help is via antigen presenting cell ( APC ) activation through engagement of CD 40 by CD 40 ligand on CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
An oral CD 40 ligand gene therapy against lymphoma using attenuated Salmonella typhimurium . ^^^ CD 40 ligand ( CD40L ) has a great potential as a novel treatment for B cell lymphoma ( BCL ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study we examined the role of a limited immune defect ( deletion or blocking of CD 40 ligand [ CD40L ] ) on the extent of brain disease , susceptibility to demyelination , and the ability of demyelinated mice to spontaneously remyelinate following TMEV infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
From these investigations , we conclude that the biology of MCL B lymphocytes is affected by CD 154 ( CD 40 ligand ) and IL 4 , two signals usually provided by CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activated DC induced cytolytic CD 8 T cells in wild type mice and also mice deficient of Th cells or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Lentiviral and murine retroviral transduction of T cells for expression of human CD 40 ligand . ^^^ The ability to produce antibodies is the consequence of interactions between T cells and B cells and lack of expression of CD 40 ligand ( CD40L ) on T cells causes 10 linked hyper IgM syndrome ( XHIM ) . ^^^ We are investigating delivery of a normal CD 40 ligand to treat this disorder . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD 154 ) triggers a short term CD 4 ( + ) T cell activation response that results in secretion of immunomodulatory cytokines and apoptosis . ^^^ Signals generated through CD 28 B7 and CD 40 ligand ( CD40L ) CD 40 interactions have been shown to be crucial for the induction of long term allograft survivability . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , in the presence of CD 40 ligand , sIgM mediated induction of CD 5 on B cells was inhibited . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD 40 ligand ( CD 154 ) engagement is required but may not be sufficient for human T helper 1 cell induction of interleukin 2 or interleukin 15 driven , contact dependent , interleukin 1beta production by monocytes . ^^^ CD 40 ligand expression levels did not differ in Th 1 and Th 2 cell clones , thus indicating that additional , unidentified molecule ( s ) preferentially expressed by Th 1 cells are involved in their IL 1beta induction capacity . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased expression of CD 40 ligand ( CD 154 ) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , MCP 1 to 4 and C5a did not suppress IL 12 production by monocyte derived dendritic cells ( DC ) stimulated with CD 40 ligand and IFN gamma or by SAC and IFN gamma , suggesting that these factors may act at the site of inflammation to suppress IL 12 and IFN gamma production rather than in the lymph node to affect T cell priming . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Neutralization experiments identified critical roles in this process for proinflammatory cytokines such as TNF alpha , IL 1beta , and IFN gamma as well as membrane molecules such as LFA 1 , CD 40 ligand , and membrane bound TNF alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction between CD 40 on B cells and CD 40 ligand ( CD40L ) on activated T cells is important for B cell differentiation in T cell dependent humoral responses . ^^^ Primary immune responses to adenoviral vectors and the ability to readminister vector were studied in rhesus monkeys in the presence or absence of a transient treatment with a humanized anti CD 40 ligand antibody ( hu5C8 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand upregulates expression of the IL 3 receptor and stimulates proliferation of B lineage acute lymphoblastic leukemia cells in the presence of IL 3 . ^^^ Here we report that CD 40 ligand ( CD40L ) in the presence of recombinant IL 3 increased proliferation of BCP ALL cells by upregulating expression of IL 3R . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Twelve cases of human AML were tested for ability to be differentiated toward a professional APC phenotype in short term culture with addition of GM CSF and the following recombinant proteins : TNFalpha , IL 4 , CD 40 ligand , Flt 3 ligand and SCF . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To determine the cellular mechanisms for control of MMP 12 expression , we studied the effects of several cytokines ( GM CSF , IL 1beta , MCP 1 ) and CD 40 ligand on MMP 12 expression in human monocyte / macrophages . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We found that sCD 14 interfered with CD 40 signaling in B cells , inhibited IL 6 production by activated B cells , and increased the kinetics and magnitude of CD 40 ligand expression on T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The cellular and humoral immune system is critically dependent upon CD 40 CD154 ( CD 40 ligand ) interactions between CD 40 expressed on B cells , macrophages , and dendritic cells , and CD 154 expressed primarily on CD 4 T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Northern blot analysis of RNA from cultured monocyte derived human DC revealed that IDO mRNA was induced upon activation with CD 40 ligand and IFN gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Following exposure to TNF alpha , LPS , or CD 40 ligand , the LCs could be synchronously induced to mature . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Role of CD 40 ligand and CD 28 in induction and maintenance of antiviral CD8+ effector T cell responses . ^^^ The primary aim of this report was to evaluate the immune responses of CD 40 ligand deficient ( CD40L / ) mice infected with two viruses known to differ markedly in their capacity to replicate in the host . ^^^ Surprisingly , lack of CD4+ T cells had no impact on the primary immune response toward any of the viruses , even though the CD 40 ligand dependence demonstrated for VSV would be expected to be associated with CD 4 dependence . ^^^ Upon coinfection of VSV infected mice with LCMV , the requirement for CD 40 ligand ( but not CD 28 ) could be partially bypassed , as evidenced by a 3 fold increase in the frequency of VSV specific CD8+ T cells on day 6 postinfection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
There , despite extensive colocalization of HEL protein and MHC class 2 products , MHC class 2 peptide complexes do not form unless the DCs are exposed to inflammatory mediators such as tumor necrosis factor alpha , CD 40 ligand , or lipoplolysaccharide . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated CD 40 ligand expressing blood T cell levels in multiple sclerosis are reversed by interferon beta treatment . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We conclude that the CD 40 ligand expressed by activated T cells first enhances MV replication in DCs , and then Fas ligand produced by activated T cells induces Fas mediated apoptosis of DCs , thus facilitating MV release . ( 2 ) Not only MV infected DCs but also bystander uninfected DCs undergo a maturation process confirmed by CD1a , CD 40 , CD 80 , CD 86 , CD 83 , and major histocompatibility complex type 2 labeling . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We tested the hypothesis whether circulating oncostatin M ( OSM ) , a cytokine that in vitro promotes fibrinogen biosynthesis and smooth muscle cell proliferation , or soluble CD 40 ligand ( CD40L ; CD 154 ) , a leukocyte and platelet surface marker that stimulates endothelial cells , were associated : ( a ) with fibrinogen and other soluble cell adhesion molecules , such as P selectin , vascular cell adhesion molecule 1 ( VCAM 1 ) , intercellular cell adhesion molecule 1 and platelet endothelial cell adhesion molecule 1 ; or ( b ) with restenosis and platelet activation in 71 patients with peripheral arterial occlusive disease undergoing peripheral angioplasty ( PTA ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Similar to mIgM signaling , all these effects are prevented by treatment with CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mice were infused with replication deficient recombinant adenovirus encoding LDLR under control of the cytomegalovirus promoter / enhancer ( AdLDLR ) , and injected with a blocking antibody directed against CD 154 ( CD 40 ligand ) to suppress immune responses against the vector and foreign transgene product . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Depressed CD 40 ligand expression contributes to reduced gamma interferon production in human tuberculosis . ^^^ Expression of CD 40 ligand ( CD40L ) correlated directly with Mycobacterium tuberculosis stimulated gamma interferon ( IFN gamma ) production by peripheral blood mononuclear cells ( PBMC ) from tuberculosis patients and healthy tuberculin reactors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Systemic upregulation of CD 40 and CD 40 ligand mRNA expression in multiple sclerosis . ^^^ It is increasingly clear that the CD 40 and CD 40 ligand ( CD40L ) receptor ligand pair mediates a crucial activation signal in both cell mediated and humoral immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The roles of CD 28 and CD 40 ligand in T cell activation and tolerance . ^^^ Costimulation of T cell activation involves both the B 7 : CD 28 as well as the CD 40 ligand ( CD40L ) : CD 40 pathway . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand induced apoptosis is Fas independent in human multiple myeloma cells . ^^^ This study characterizes the growth outcome of soluble ( gp 39 ) or membrane bound recombinant human CD 40 ligand ( rCD40L ) and its relationship with Fas dependent apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
MIP T 3 complex and recruited to the CD 40 receptor upon CD 40 ligand stimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , the third signal is provided via the interaction between the constitutively expressed CD 40 molecule on B lymphocytes and CD 154 ( CD 40 ligand ) , a molecule expressed on T lymphocytes following activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This prothrombotic , proinflammatory state is characterised by vaso constriction , platelet and leukocyte activation and adhesion ( externalization , expression and upregulation of von Willebrand factor , platelet activating factor , P selectin , ICAM 1 , IL 8 , MCP 1 , TNF alpha , etc . ) , promotion of thrombin formation , coagulation and fibrin deposition at the vascular wall ( expression of tissue factor , PAI 1 , phosphatidyl serine , etc . ) and , in platelet leukocyte coaggregates , additional inflammatory interactions via attachment of platelet CD 40 ligand to endothelial , monocyte and B cell CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Specimens were analyzed in a masked manner by flow cytometry , using antibodies directed to markers of the immune system and / or apoptotic pathway : HLA DR , CD 40 , CD 40 ligand , Fas , and APO2 . 7 . ^^^ CD 40 and CD 40 ligand expressions were significantly increased in eyes with KCS compared with normal eyes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS AND RESULTS : We investigated whether specific antigenic stimulation of Ts is required for their ability to inhibit early activation of xenoreactive Th ( up regulation of CD 40 ligand ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In these IL 2 supplemented cocultures , no effect on CTL generation or phenotype was observed by mAb blocking of costimulation provided by LFA 3 , ICAM 1 , or CD 40 , by addition of comitogenic anti CD 28 mAb , or by preactivation of EC with CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B lymphocyte responses appear thus controlled by different T cell subsets expressing CD 154 ( CD 40 ligand ) , CD 27 , or CD 70 ( CD 27 ligand ) . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Further engineering of DCs to express either GM CSF , tumor necrosis factor alpha , or CD 40 ligand via retroviral mediated gene transfer , led to a significantly increased therapeutic effect in the subcutaneous tumor model . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The ADAM 10 was catalytically active , cleaving myelin basic protein and peptides based on the previously described ' metallosheddase ' cleavage sites of tumour necrosis factor alpha , CD 40 ligand and amyloid precursor protein . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , using intracellular staining and flow cytometry to analyze cytokine production at the single cell level , we found that Lm harboring DC , but not monocytes , produce large amounts of IL 12p70 in a CD 40 ligand ( CD40L ) dependent manner . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Because CD 40 ligand ( CD40L ) can be expressed on numerous cell types in addition to activated T cells , it is possible that cells other than T cells provide CD40L to signal through CD 40 on B cells and hence positively influence the antiviral TI IgG responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD 154 ) enhances the Th 1 and antibody responses to respiratory syncytial virus in the BALB / c mouse . ^^^ CD 40 ligand ( CD40L ) is a cell surface costimulatory molecule expressed mainly by activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CBMC IL 12 levels were increased by interferon gamma and CD 40 ligand but remained deficient , compared with PBMC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Nucleotide sequence of the rat CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 using anti CD 40 or soluble CD 40 ligand activates numerous intracellular kinases which transduce signals to the nucleus . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Several functional parameters were assayed in response to both membrane ( anti CD 3 and IL 2 ) and transmembrane ( phorbol myristate acetate plus the calcium ionophore ionomycin ) stimuli : ( 1 ) calcium mobilization , ( 2 ) induction of activation molecules ( CD 25 , CD 40 ligand , CD 69 and CD 71 ) , ( 3 ) cytokine synthesis ( IL 2 and tumor necrosis factor alpha ) and ( 4 ) proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD 40 ligand interactions in vivo regulate migration of antigen bearing dendritic cells from the skin to draining lymph nodes . ^^^ We analyzed the DC status of CD 40 ligand / mice using a contact hypersensitivity ( CHS ) model system that enables multiple functions of DCs to be assessed in vivo . ^^^ Immunohistochemistry of skin sections revealed no differences in terms of numbers and morphology of dendritic epidermal Langerhans cells ( LCs ) in unsensitized CD 40 ligand / mice as compared with wild type C57BL / 6 mice . ^^^ However , after contact sensitization of CD 40 ligand / mice , LCs failed to migrate out of the skin and substantially fewer DCs accumulated in draining lymph nodes ( DLNs ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the present study we examined the expression and involvement of CD 40 and CD 40 ligand ( CD40L ) in the interaction between muscle cells and T cells in PM / DM . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using ELISA as well as real time quantitative PCR , we show that cultured primary keratinocytes , dermal fibroblasts , and dermal microvascular endothelial and dendritic cells are major sources of CCL 20 , and that the expression of this chemokine can be induced by proinflammatory mediators such as TNF alpha / IL 1 beta , CD 40 ligand , IFN gamma , or IL 17 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) , a membrane protein expressed on activated T cells , plays a pivotal role in B cell proliferation and differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased expression of CD 40 and CD 40 ligand ( CD40L or CD 154 ) has been found in inflamed mucosa of human inflammatory bowel disease ( IBD ) , and interactions between these molecules seem to be involved in local cytokine production by macrophages . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study explored the role of CD 40 / CD 40 ligand ( CD40L ) in the induction of a lymphoproliferative response and killing of Cryptococcus neoformans in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Up regulation of T cell co stimulatory receptors CD11a , CD 40 ligand ( CD40L ) and CTLA 4 were inhibited in a dose dependent manner by plasma derived ( pd ) FVIII , but CD 28 was unchanged . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This effect of IL 6 was blocked by tumour necrosis factor alpha ( TNF alpha ) , lipopolysaccharide ( LPS ) , IL 1beta , CD 40 ligand ( CD40L ) and transforming growth factor beta 1 ( TGF beta 1 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recently , we and others have implicated the immune mediator dyad CD40 / CD40L ( CD 40 ligand ) , which is expressed on endothelial and smooth muscle cells , macrophages , and T lymphocytes within human atherosclerotic lesions , in aspects of atherogenesis and the acute coronary syndromes , including regulation of matrix metalloproteinases , procoagulant activity , cytokines , etc . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD11c+ and CD11c cells can mature in vitro with GM CSF + TNF alpha or with IL 3 + CD 40 ligand , respectively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study presents a functional analysis of the CD 4 ( + ) T cell response to EBV and shows that while CD 4 ( + ) T cells may be cytotoxic , they also express Th 2 cytokines and CD 40 ligand ( gp 39 ) and possess B cell helper function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Most notably , the DCAdhgp 100 vaccine could elicit protective antitumor CD4+ cells in the absence of CD 40 ligand , although it does not bypass the need for CD 40 mediated signals to generate melanoma reactive CTLs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , a previously unreported potentially disease causing mutation in the CD 40 ligand gene was detected in this patient . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
High dose porcine hematopoietic cell transplantation combined with CD 40 ligand blockade in baboons prevents an induced anti pig humoral response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand stimulates myelopoiesis by regulating flt 3 ligand and thrombopoietin production in bone marrow stromal cells . ^^^ CD 40 ligand ( CD40L ) / CD40 interactions play a central role in T cell dependent B cell activation as previously shown by in vitro studies , the phenotype of CD40L knockout mice and the defective expression of CD40L in patients who have 10 linked immunodeficiency with hyper IgM . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we show that IgD+ IgM+ B cells up regulate CD 153 in the presence of CD 154 ( CD 40 ligand ) , IL 4 , and B cell Ag receptor engagement . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Parasite growth was not enhanced over the first 4 . 5 wk in anti CD 4 treated mice , SCID mice , or C57BL / 6 mice deficient in IL 12p40 , IFN gamma , CD 40 ligand , or inducible NO synthase . ^^^ These mice all failed to ultimately control infection in the site , but in some cases ( anti CD 4 treated , IL 12p40 / , CD 40 ligand / , and SCID ) high dermal parasite loads were associated with little or no pathology . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dendritic cells modified to express CD 40 ligand elicit therapeutic immunity against preexisting murine tumors . ^^^ CD 40 ligand ( CD40L ) is essential for the initiation of antigen specific T cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using a trimeric recombinant soluble CD 40 ligand to activate CD 40 , we have found that this phenomenon critically depends on the membrane proximal domain ( amino acids 216 to 239 ) but not the TNFR associated factor interacting PXQXT motif in the CD 40 cytoplasmic tail . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These results support hypotheses that the proliferation of EBV infected B cells in vivo can be inhibited by interactions with the CD 40 ligand on activated helper T cells , and latent EBV is reactivated via the signaling pathways controlled by CD 40 interactions . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Platelet activation was measured on days 1 , 3 , 7 and 9 by flow cytometry using fluoresceinisothiocyanate labeled monoclonal antibodies ( mAbs ) against glycoprotein IIb / IIIa ( CD41a , PAC 1 and LIBS 1 ) , P selectin ( CD62P ) or CD 40 ligand receptor ( CD40L ) in combination with a phycoerythrin labeled panspecific platelet marker against GPIb ( CD42b ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immature DCs can be generated from monocytes with GM CSF and IL 4 , while maturation is accomplished by the addition of stimuli such as monocyte conditioned medium , CD 40 ligand , and LPS . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human dendritic cells discriminate between viable and killed Toxoplasma gondii tachyzoites : dendritic cell activation after infection with viable parasites results in CD 28 and CD 40 ligand signaling that controls IL 12 dependent and independent T cell production of IFN gamma . ^^^ IFN gamma production in response to DC infected with viable parasites required CD 28 and CD 40 ligand ( CD40L ) signaling . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand trimer and IL 12 enhance peripheral blood mononuclear cells and CD4+ T cell proliferation and production of IFN gamma in response to p 24 antigen in HIV infected individuals : potential contribution of anergy to HIV specific unresponsiveness . ^^^ CD 40 ligand trimer ( CD40LT ) and IL 12 significantly increased PBMC and CD4+ T cell proliferative responses to p 24 Ag in HIV infected , but not uninfected , individuals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This response was induced not only by endotoxin but also by bacillus Calmette Guerin ( BCG ) and CD 40 ligand and was associated with ( and likely caused by ) the enhanced and prolonged expression of p 40 mRNA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Pathogenic Bir T cell lines expressed CD 40 ligand ( CD40L ) when cultured with Ag pulsed APCs in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Biological function of CD 40 on human endothelial cells : costimulation with CD 40 ligand and interleukin 4 selectively induces expression of vascular cell adhesion molecule 1 and P selectin resulting in preferential adhesion of lymphocytes . ^^^ Here we show that costimulation with CD 40 ligand ( CD40L ) and interleukin ( IL ) 4 ( or IL 13 ) gives rise to a unique pattern of adhesion molecule expression by human umbilical vein endothelial cells ( HUVEC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In a C . pneumoniae infected plaque , such T cells may be activated by local antigen and could contribute to the inflammatory process in the arterial wall through CD 40 ligand expression and cytokine secretion . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These results suggest that development of Th 1 or Th 2 memory cells in patients with XHIGM is unaffected by the absence of functional CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We report the clinical and immunologic features and outcome in 56 patients with 10 linked hyper IgM syndrome , a disorder caused by mutations in the CD 40 ligand gene . ^^^ Although lymphocyte counts and in vitro proliferation to mitogens were normal , a defective in vitro proliferative response to antigens was observed in some patients , and additional defects of cell mediated immunity may be presumed on the basis of current knowledge of CD 40 ligand function . ^^^ We describe 5 children from 2 families with mutations in the CD 40 ligand ( CD40L ) gene leading to absent expression of CD40L on activated CD 4 cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : We investigated the contribution of antigen specific and CD 40 ligand ( CD40L ) mediated signals to chemical allergen induced CD 4 T cell growth and cytokine production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
At a low ratio ( 1 : 300 ) , mature DCs that have been differentiated after inflammatory ( Staphylococcus aureus Cowan 1 or lipopolysaccharide ) or T cell dependent ( CD 40 ligand ) stimulation induced naive T cells to become Th 2 ( interleukin [ IL ] 4 ( + ) , IL 5 ( + ) , interferon gamma ) effectors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased expression of CD 40 ligand in activated CD4+ T lymphocytes of systemic sclerosis patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recently it has been demonstrated that these kidney infiltrating T cells express CD 40 ligand and that tubular epithelial cells ( TECs ) express CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dex DC were unresponsive to signals inducing maturation ( CD 40 ligand , lipopolysaccharide ) , as demonstrated by the absence of CD 83 , CD80 / CD86 and HLA DR up regulation and their strongly reduced T cell stimulatory capacity . ^^^ Furthermore , Dex DC showed a decreased CD 40 ligand induced IL 6 and TNF alpha production , a complete block in IL 12p40 production , while IL 10 production was unaffected . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vivo administration of IL 18 can induce IgE production through Th 2 cytokine induction and up regulation of CD 40 ligand ( CD 154 ) expression on CD4+ T cells . ^^^ IL 18 can rapidly induce CD 40 ligand ( CD 154 ) mRNA and surface expression on CD4+ but not CD8+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The antigen dose determines T helper subset development by regulation of CD 40 ligand . ^^^ Using co cultures of dendritic cells ( DC ) and ovalbumin ( OVA ) specific CD4+ T cells obtained from RAG 2 ) ( / ) DO11 . 10 mice , we show here that high dose antigen induced Th 1 development by up regulation of CD 40 ligand ( CD40L ) , whereas low dose antigen stimulation failed to induce CD40L and promoted Th 2 development . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Two patients with CD 40 ligand deficiency now show significant expression , and a patient with adenosine deaminase deficiency has improved deoxy adenosine triphosphate metabolites . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study has identified the incidence and clinical significance of high CD 86 expression on plasma cells at diagnosis and studied the ability of trimeric human CD 40 ligand ( huCD40LT ) to up regulate the expression of the B 7 family on malignant plasma cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of DC for 24 hours with CD 40 ligand ( CD 154 ) , IL 12 or IL 15 prior to their co incubation with prostate cancer cells resulted in a significant increase in DC survival in the tumour microenvironment . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Resting and activated T cells induce expression of E selectin and VCAM 1 by vascular endothelial cells through a contact dependent but CD 40 ligand independent mechanism . ^^^ The EC stimulating capacity of resting or activated T cells was independent of CD 40 ligand . ^^^ Furthermore , inhibition of TNF alpha / beta and IL 1alpha / beta , together with CD 40 ligand , failed to inhibit EC activation by resting T cells and only inhibited the response to PMA and ionomycin activated T cells by 40 + / 18 % . ^^^ Our data suggest that T cell EC interactions can lead to EC activation through a novel contact dependent , but CD 40 ligand independent , mechanism . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Heightened CD 40 ligand gene expression in peripheral CD4+ T cells from patients with kidney allograft rejection . ^^^ BACKGROUND : CD 40 ligand ( CD40L ) gene expression is increased in rejecting allograft biopsies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human immunodeficiency virus type 1 infection modulates the interleukin ( IL ) 1beta and IL 6 responses of human macrophages to CD 40 ligand stimulation . ^^^ The effect of HIV 1 infection on recombinant CD 40 ligand ( CD40L ) induced interleukin ( IL ) 1beta and IL 6 production by human macrophages was analyzed . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Since CD 40 ligand is early up regulated on peripheral blood basophils ( p < 0 . 05 ) , it may be critical in the initial IgE production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of CD 40 ligand and other immunomodulators on Pneumocystis carinii infection in rat model . ^^^ In this study the effects of five immunomodulators ; recombinant CD 40 ligand ( CD40L ) , bryostatin 1 , recombinant FLT 3 ligand ( FLT3L ) , recombinant granulocyte colony stimulating factor ( G CSF ) and recombinant interleukin 15 ( IL 15 ) were investigated against PCP in a dexamethasone immunosuppressed Sprague Dawley rat model . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Tumor necrosis factor alpha and CD 40 ligand antagonize the inhibitory effects of interleukin 10 on T cell stimulatory capacity of dendritic cells . ^^^ In contrast , the simultaneous incubation of monocytes with IL 10 and TNF alpha or soluble CD 40 ligand ( sCD40L ) resulted in the generation of CD 83 positive DCs , induction of nuclear localized RelB , and inhibition of IL 10R up regulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Host CD 40 ligand deficiency induces long term allograft survival and donor specific tolerance in mouse cardiac transplantation but does not prevent graft arteriosclerosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Further , the changes of these markers and costimulatory molecules [ LFA 1 ( CD11a ) , ICAM 1 ( CD 54 ) , CD 40 and CD 40 ligand ( CD 154 ) ] were also examined in the responsive patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The emerging role of CD 40 ligand in HIV infection . ^^^ CD 40 ligand ( also called CD40L , CD 154 , or TNFSF 5 ) is a membrane protein expressed mainly by activated CD4+ T cells , which interacts with its receptor , CD 40 , on a variety of cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Also discussed is the decreased CD 40 ligand induction in CD 4 T cells during HIV infection , which may have a strong impact on T cell dependent IL 12 production that is critical for the establishment and maintenance of a Th 1 response . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transduced cells showed characteristic dendritic cell phenotype and strong allostimulatory capacity and maintained the ability to respond to activation signals such as CD 40 ligand and lipopolysaccharide . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD14 ( + ) monocytes were selected by high gradient magnetic separation and differentiated to immature dendritic cells with granulocyte macrophage colony stimulating factor and interleukin 4 for 6 7 days and then induced to terminal maturation by the addition of tumor necrosis factor alpha or stimulation with CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The cytokines FLT3L + SCF + IL 7 + IGF 1 were added at day 0 , IL 4 + IL 5 + IL 6 + IL 10 and soluble CD 40 ligand at day 14 , and Staph . aureus Cowan particles on day 21 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vivo gene transfer of CD 40 ligand into colon cancer cells induces local production of cytokines and chemokines , tumor eradication and protective antitumor immunity . ^^^ The interaction between CD 40 ligand ( CD40L , CD 154 ) and its receptor CD 40 on antigen presenting cells , is essential for the initiation of cell mediated and humoral immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A cell surface cytokine CD 40 ligand ( CD40L ) enhances TF expression in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of agonist induced p 42 and p 38 mitogen activated protein kinase phosphorylation and CD 40 ligand / P selectin expression by cyclic nucleotide regulated pathways in human platelets . ^^^ Platelet activation is initiated by stimulation of intracellular signaling cascades , including the p 42 mitogen activated protein kinase ( MAPK ) and p 38 MAPK pathways , followed by major changes in the platelet cytoskeleton and expression and activation of platelet surface receptors , such as P selectin ( CD62P ) and CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When cultured with anti IgM maintained at high density on CD 32 tranfected mouse L cells to model TI responses or on CD 40 ligand ( CD40L ) bearing L cells ( with or without captured anti IgM ) to model TD encounters , DNA synthesis was stimulated to a similar extent in all three populations . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BLyS acts on primary splenic B cells autonomously , and directly cooperates with CD 40 ligand ( CD40L ) in B cell activation in vitro by protecting replicating B cells from apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dendritic cells genetically modified to express CD 40 ligand and pulsed with antigen can initiate antigen specific humoral immunity independent of CD4+ T cells . ^^^ We have investigated whether dendritic cells genetically modified to express CD 40 ligand and pulsed with antigen can trigger B cells to produce antigen specific antibodies without CD4+ T cell help . ^^^ Dendritic cells modified with a recombinant adenovirus vector to express CD 40 ligand and pulsed with heat killed Pseudomonas induced naive B cells to produce antibodies against Pseudomonas in the absence of CD4+ T cells in vitro , initiated Pseudomonas specific humoral immune responses in vivo in wild type and CD 4 / mice , and protected immunized wild type and CD 4 / , but not B cell / mice , from lethal intrapulmonary challenge with Pseudomonas . ^^^ Thus , genetic modification of dendritic cells with CD 40 ligand enables them to present a complex mixture of microbial antigens and establish CD4+ T cell independent , B cell mediated protective immunity against a specific microbe . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of T lymphocyte costimulatory pathways was evaluated using anti CD 40 ligand or anti B 7 1 and B 7 2 monoclonal blocking antibodies . ^^^ Intrapancreatic interleukin 12 , interferon gamma , Fas ligand , and CD 40 ligand messenger RNA were detected by reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand induces beta chemokine production by macrophages and resistance to HIV 1 entry . ^^^ CD 40 ligand ( CD40L ) is a cell surface molecule of CD 4 ( + ) T cells that interacts with its receptor CD 40 on antigen presenting cells to mediate thymus dependent humoral immunity and inflammatory reactions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Role of platelet P selectin and CD 40 ligand in the induction of monocytic tissue factor expression . ^^^ Activated platelets can express CD 40 ligand ( CD40L ) and trigger inflammatory response and tissue factor ( TF ) expression in endothelial cells through interaction with CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of CTL and elimination of allogeneic P 815 cells were inhibited both in the presence and absence of CD 4 cells by temporary CD 40 ligand blockade . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD4+ T lymphocytes with constitutive CD 40 ligand in preautoimmune ( NZB 10 NZW ) F 1 lupus prone mice : phenotype and possible role in autoreactivity . ^^^ One possible mechanism is suggested by studies showing that human patients with systemic lupus erythematosus and lupus mice have increased numbers of CD 40 ligand ( CD40L ) + T and B lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
COX 2 is functional in MDC as a specific inhibitor , NS 398 , significantly reduces PGE ( 2 ) production . 1 MDC undergoing maturation with soluble CD 40 ligand ( sCD40L ) increase PGE ( 2 ) synthesis , but prostanoid synthesis is switched to COX 1 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro stimulation by Staphylococcus aureus Cowan 1 strain or CD 40 ligand associated with IL 10 and IL 2 induces a rapid decrease in p 27 ( Kip 1 ) expression combined with cell cycle entry and progression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Abundant expression of CD 40 and CD 40 ligand ( CD 154 ) in paediatric Langerhans cell histiocytosis lesions . ^^^ The interaction between CD 40 on antigen presenting cells ( APC ) like Langerhans cells and CD 40 ligand ( CD40L ) ( CD 154 ) expressed by activated CD4+ T cells , is essential for the activation of both the APC and the T cells and results in upregulation of APC functions and initiation of immunoreactivity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand expression on macrophages during peritonitis in continuous ambulatory peritoneal dialysis patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Nondepleting anti CD 154 ( CD 40 ligand ) mAbs have proven effective in inducing transplantation tolerance in rodents and primates . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TRAF 1 was also highly inducible by CD 40 ligand in cultured germinal center B cells , whereas TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 were relatively unchanged . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Whole tumor vaccination strategies are also being examined and include the use of MM cells transfected and / or stimulated with cytokines , costimulatory molecules , or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interleukin 17 and CD 40 ligand synergistically enhance cytokine and chemokine production by renal epithelial cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast to these effects , CD 40 stimulation by either anti CD 40 antibodies or a recombinant soluble CD 40 ligand can inhibit the growth of human breast carcinomas and aggressive histology B lymphomas in vitro and in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Osteopontin augments CD 3 mediated interferon gamma and CD 40 ligand expression by T cells , which results in IL 12 production from peripheral blood mononuclear cells . ^^^ Herein , we demonstrate that osteopontin stimulation augments the ability of anti CD 3 monoclonal antibody to induce CD 40 ligand ( CD40L ) and IFN gamma expression on human T cells , resulting in CD40L and IFN gamma dependent IL 12 production in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The available measurements to be used include up regulation of CD1d , administration of relative cytokines , administration of Freund adjuvant , administration of monoclonal antibodies against cytokines , cytokine receptors , CD 40 ligand or B 7 molecule , as well as CTLA or CTLAIg pathway and so on . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand in CLL pathogenesis and therapy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand , Bcl 2 , and Bcl xL spare group 1 Burkitt lymphoma cells from CD 77 directed killing via Verotoxin 1 B chain but fail to protect against the holotoxin . ^^^ Here we show that CD 77 expressing Group 1 BL cells which are normally susceptible to activation induced death on binding Verotoxin 1 B chain are protected in the presence of CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 and CD 40 ligand ( CD40L ) have been implicated as important molecules for the transformation of nonactivated antigen presenting cells ( APC ) into cells that are potent inducers of cytotoxic T lymphocyte ( CTL ) immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction of proinflammatory type 1 T helper ( Th 1 ) cells expressing the CD 40 ligand ( CD 154 ) with endothelial cells expressing the corresponding receptor ( CD 40 ) may play an important role in chronic inflammation including arteriosclerosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A subset of human monocyte derived dendritic cells expresses high levels of interleukin 12 in response to combined CD 40 ligand and interferon gamma treatment . ^^^ We used combinations of signaling through CD 40 , using CD 40 ligand trimer ( CD40L ) , and interferon gamma to demonstrate that CD 83 expression is necessary but not sufficient for optimal production of IL 12 by DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD 40 ligand and tumor necrosis factor alpha signaling in the transgenic K / BxN mouse model of rheumatoid arthritis . ^^^ This study sought to analyze the potential of anti CD 40 ligand ( anti CD40L ) and anti tumor necrosis factor alpha ( anti TNFalpha ) antibodies in preventing and treating arthritis in this murine model . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD4+ T cell help and CD 40 ligand in the in vitro expansion of HIV 1 specific memory cytotoxic CD8+ T cell responses . ^^^ Also , CD 40 ligand trimer ( CD40LT ) enhanced CTL responses and was able to completely substitute for CD 4 ( + ) T cell help in PBMCs from normal volunteers . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The infected populations were further matured with CD 40 ligand , leading to strong stimulation of autologous T cells from HIV 1 infected individuals , but not controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulatory defects in Cbl and mitogen activated protein kinase ( extracellular signal related kinase ) pathways cause persistent hyperexpression of CD 40 ligand in human lupus T cells . ^^^ Lupus T cells showed prolonged high level expression of CD 40 ligand ( CD40L , CD 154 ) even in the face of anergy protocol , which shut down CD40L expression in normal T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , treatment of HUVECs and monocytes with soluble CD 40 ligand ( sCD40L ) resulted in a marked dose dependent induction of VEGF mRNA ( approximately 4 fold ) , which peaked between 1 and 5 hours post stimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Lymphoid abnormalities in CD 40 ligand transgenic mice suggest the need for tight regulation in gene therapy approaches to hyper immunoglobulin M ( IgM ) syndrome . ^^^ Mutations in the CD 40 ligand ( CD40L ) are responsible for human hyper immunoglobulin M ( IgM ) syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The B cell activating properties of R 848 are strikingly similar to the effects of the CD 40 ligand CD 154 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DC were also cultured with L cells expressing human CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When cultured monocyte derived DC received stimulation with CD 40 ligand ( CD40L ) and lipopolysaccharide ( LPS ) together , the IL 12 secretion increased 5 60 fold and the IL 10 secretion increased 5 15 fold when compared with either stimulation alone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Hyper IgM immunodeficiency ( HIM ) is an immunological disorder characterized by normal or elevated serum IgM levels , and reduced serum IgG and IgA levels , due to the disruption of immunoglobulin class switching in B cells . 10 linked hyper IgM is caused by the defective expression of the CD 40 ligand on activated T cells , which induces immunoglobulin class switching along with some cytokines , such as interleukin 4 , by the signal transduction of CD 40 in B cells . ^^^ No mutation of the CD 40 ligand or CD 40 was detected . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
While IL 12 production induced during culture of 5 ( alpha ) 14 ( + ) NKT cells and APC depended on the interaction between CD 40 ligand on NKT cells and CD 40 on APC , the expression levels of these key molecules were comparable in cells from wild type and IFN gamma ( / ) mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In secondary lymphoid organs , CD 4 ( + ) CXCR 5 ( + ) cells lose expression of CCR 7 , which allows them to localize to B cell follicles and germinal centers where they express high levels of CD 40 ligand ( CD40L ) , a costimulatory molecule required for B cell activation and inducible costimulator ( ICOS ) , a recently identified costimulatory molecule of the CD 28 family . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Tg mice immunized with the FC / MUC1 fusion cells proliferate in response to MUC 1 antigen by a mechanism dependent on the function of CD 4 , major histocompatibility complex ( MHC ) class 2 , B 7 1 , B 7 2 , CD 28 , CD 40 and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To determine the relative frequency of mutations in AID , we evaluated a group of 27 patients with hyper IgM syndrome who did not have defects in CD 40 ligand and 23 patients with common variable immunodeficiency . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transcriptional activity of the distal CD 40 ligand promoter . ^^^ CD 40 ligand ( CD40L , CD 154 ) is a T cell cytokine with highly regulated expression that requires the transcription factor nuclear factor of activated T cells ( NF AT ) to bind at two sites in the proximal CD40L promoter . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 receptor , which can transduce survival signals in mature malignant B cells , was less frequently expressed on ALL cells , but incubation with an exogenous soluble CD 40 ligand trimer did not rescue them from spontaneous apoptosis and did not mediate their resistance to TRAIL . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NK cell activation by anti CD 40 Ab was also observed in CD 40 ligand knockout mice . ^^^ Because NK cells express CD 40 ligand but not CD 40 , our results suggest that NK activation is mediated by increased cytokine production upon CD 40 ligation of APCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro , RANTES up regulated the expression of CD 28 , CD 40 ligand , and IL 12R by Ag activated primary T cells from DO11 . 10 ( OVA specific TCR transgenic ) mice and by resting T cells in a dose dependent fashion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Targeting signal 1 through CD45RB synergizes with CD 40 ligand blockade and promotes long term engraftment and tolerance in stringent transplant models . ^^^ Although combined blockade of CD 28 and CD 40 ligand ( CD40L ) costimulatory pathways prevents allograft rejection in some murine models , this strategy is unable to sustain engraftment in the most immunogenic allograft and strain combinations . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mutations in the CD 40 ligand ( CD40L ) gene lead to 10 linked immunodeficiency with hyper IgM , which is often associated with autoimmune diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of germline ( GL ) epsilon transcripts , an essential step preceding Ig isotype switching to IgE , requires activation of transcription factors by IL 4 and a B cell activator , e . g . , CD 40 ligand or LPS . ^^^ We demonstrate that AP 1 ( Fos and Jun ) , induced transiently by CD 40 ligand or LPS , binds a DNA element in the mouse GL epsilon promoter . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of the T cell costimulatory molecule CD 154 ( CD 40 ligand ) has been effective in producing long term allograft survival in rodents and non human primates . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 and CD 40 ligand ( CD40L ) interaction has been implicated in the generation of effective cell mediated and humoral immune responses , where cytokines have been shown to play a significant role in the expression of these molecules . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Specimens were processed and analyzed in a masked manner by flow cytometry , using monoclonal antibodies directed to HLA DR , CD 40 , CD 40 ligand , Fas , and the apoptotic marker APO2 . 7 . ^^^ CD 40 ligand expression also decreased at months 3 and 6 in patients taking both concentrations of cyclosporin A . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A role in the rejection process is also suggested for the interaction between CD 40 ligand ( CD 154 ) expressed on membranes of activated T cells and its receptor ( CD 40 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dependence of the primary antiviral immune response on costimulatory interactions between CD28 / CD80 86 and between CD40 / CD154 ( CD 40 ligand ) has been correlated with the extent of viral replication in two models of systemic infection , lymphocytic choriomeningitis virus and vesicular stomatitis virus . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we used murine CD 154 ( CD 40 ligand ) and gamma chain common cytokines to support the growth of B cells isolated from ovine lymphoid tissues . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These patients , who do not express a functional CD 40 ligand , can not switch Ig isotypes and do not form germinal centers and memory B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Glucocorticoids upregulate CD 40 ligand expression and induce CD40L dependent immunoglobulin isotype switching . ^^^ In this study we demonstrate that HC fails to induce IgE synthesis in B cells from CD 40 ligand deficient ( CD40L deficient ) patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that stimulation through the TCR and CD 28 or CD 40 ligand directly leads to T cell activation and several rounds of proliferation in an IL 2 independent fashion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
PP B cells can be induced to express CD 38 upon culture with CD 40 ligand , anti B cell receptor , and IFN gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Identification of a CD 28 response element in the CD 40 ligand promoter . ^^^ Ligation of the T cell coreceptor CD 28 or CD 2 by its cognate ligands B 7 1 or LFA 3 , respectively , greatly aids the Ag induced up regulation of several genes , including IL 2 and CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Although immature DC can be derived in vitro by treatment of peripheral blood monocytes with GM CSF and IL 4 , additional signals such as those provided by TNF alpha , CD 40 ligand , or LPS are required for complete maturation and maximum APC function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These leukemia induced CD30+ T cells inhibit CD 40 ligand ( CD40L ) mediated S mu > S gamma and S mu > S alpha class switch DNA recombination ( CSR ) by engaging CD 30 ligand ( CD30L ) , a molecule that interferes with the assembly of the CD 40 tumor necrosis factor receptor associated factor ( TRAF ) complex in nonmalignant IgD+ B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Low levels of productive HIV infection in Langerhans cell like dendritic cells differentiated in the presence of TGF beta 1 and increased viral replication with CD 40 ligand induced maturation . ^^^ Here , morphologic , phenotypic , functional and HIV infection experiments were performed using monocyte derived DC cultured in the presence of GM CSF , IL 4 and TGF beta 1 ( G4T DC ) , GM CSF and IL 4 ( G 4 DC ) , and G4T DC incubated for an additional 3 days with CD 40 ligand ( CD40L DC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Second , mutant B cell clones with improved affinity for Ag are positively selected by Ag and CD 40 ligand ( L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thus while CD4+ cells are not critical during the initial phase of virus control , infectious virus reappear in mice lacking CD4+ cells , B cells or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of CD 40 ligand ( CD 154 ) in the treatment of factor 8 inhibitors . ^^^ One cognate receptor pair that is required for T cell dependent B cell activation consists of CD 40 , which is expressed on B lymphocytes and other antigen presenting cells , and CD 40 ligand ( CD40L , CD 154 ) , which is transiently expressed on activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand expression decreased . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vivo CD 40 CD154 ( CD 40 ligand ) interaction induces integrated HIV expression by APC in an HIV 1 transgenic mouse model . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Among other dissimilarities , neoplastic T cells showed coexpression of CD 40 ( Bp 50 ) and CD 40 ligand ( gp 39 , CD 154 ) . ^^^ Coexpression of CD 40 and CD 40 ligand in cutaneous T cell lymphoma ( mycosis fungoides ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These mutations prevent CD 40 ligand ( CD40L ) mediated degradation of inhibitor of NF kappaB alpha ( IkappaB alpha ) and account for the following observations : B cells from XHM ED patients are unable to undergo immunoglobulin class switch recombination and antigen presenting cells ( APCs ) are unable to synthesize the NF kappaB regulated cytokines interleukin 12 ( IL 12 ) or tumor necrosis factor alpha ( TNF alpha ) when stimulated with CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In systemic lupus erythematosus ( SLE ) , T helper cells exhibit increased and prolonged expression of cell surface CD 40 ligand ( CD 154 ) , spontaneously overproduce interleukin 10 ( IL 10 ) , but underproduce interferon gamma ( IFN gamma ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immune mediator CD 40 ligand is expressed on a variety of cell types involved in the immune response and on the cells of the vascular system . ^^^ After carotid artery injury in CD 40 ligand knockout ( CD40L ( / ) ) mice , the intimal thickening was increased 3 fold compared with the thickening in background B6 / 129 mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunologic reconstitution following bone marrow transplantation for 10 linked hyper IgM syndrome . 10 linked hyper IgM syndrome ( XHIM ) , caused by mutations of the CD 40 ligand ( CD40L ) gene , is characterized by recurrent bacterial and opportunistic infections , an increased incidence of autoimmunity and malignancies , and immunodeficiency due to abnormal T / B cell interaction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Pro inflammatory stimuli known to be present in human atherosclerotic plaque , including CD 40 ligand , lysophosphatidylcholine , or cholesterol crystals , could induce release of myeloperoxidase from HOCl production by macrophages in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon culture with IL 3 , granulocyte / macrophage colony stimulating factor , and CD 40 ligand , the plasmacytoid cells can adopt a phenotype resembling that of freshly isolated CD11b thymic DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Up regulation of CD 40 ligand and induction of a Th 2 response in children immunized with pneumococcal polysaccharide vaccines . ^^^ We wished to determine whether pneumococcal polysaccharide antigens induce mRNA expression of CD 40 ligand ( CD40L ) and Th 1 or Th 2 cytokines in unimmunized individuals in vitro and whether immunization with the 23 valent pneumococcal polysaccharide vaccine induces changes in CD40L and cytokine mRNA expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we compared the early phases of this cycle in immature DC ( iDC ) and mature DC ( mDC ) generated from monocytes cultured with GM CSF and IL 4 , trimeric CD 40 ligand ( DC ( CD40LT ) ) , or monocyte conditioned medium ( DC ( MCM ) ) being added or not from day 5 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Signals that induce maturation ( lipopolysaccharide , IL 1 , TNF , CD 40 ligand , recognition of bacteria and yeast ) dramatically augmented MDC production , and dexamethasone and vitamin D 3 blocked it . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand an important target for immunotherapy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To generate CTLs specific for both AdV and EBV , we developed a CD 40 ligand co culture system to infect B lymphoblastoid cell lines ( LCLs ) with high efficiency . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mycobacterium bovis BCG induced granuloma formation depends on gamma interferon and CD 40 ligand but does not require CD 28 . ^^^ In order to understand the costimulatory requirements that allow CD4+ T cells to directly or indirectly induce granulomas , we studied granuloma formation after 6 weeks in Mycobacterium bovis BCG infected CD 28 and CD 40 ligand ( CD40L ) deficient mice and compared it to granuloma formation in infected wild type inbred mice and infected cytokine deficient mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After stimulation with bacteria , LPS , lipoteichoic acid , or soluble CD 40 ligand , DCs secreted high levels of IL 10 . ^^^ Addition of an anti IL 10 neutralizing Ab to immature DCs as well as to soluble CD 40 ligand or LPS maturing DCs led to enhanced expression of surface CD 83 , CD 80 , CD 86 , and MHC molecules and markedly augmented release of TNF alpha and IL 12 , but diminished IL 10 mRNA expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of transcriptional activity of the murine CD 40 ligand promoter in response to signals through TCR and the costimulatory molecules CD 28 and CD 2 . ^^^ We have analyzed the murine CD 40 ligand promoter with regard to stimulation of transcriptional activity in Jurkat T cells after signaling via the TCR and the costimulatory molecules CD 28 and CD 2 . ^^^ TCR engagement was necessary for the induction of transcriptional activity from the CD 40 ligand promoter , and costimulation through either CD 28 or CD 2 further increased the activity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We demonstrated that activated CD4+ Valpha24NKT cells [ CD 40 ligand ( CD40L ) positive ] have cytotoxic activity against DCs ( strongly CD 40 positive ) , but not against monocytes ( weakly CD 40 positive ) or phytohaemagglutinin blast T cells ( CD 40 negative ) , and that apoptosis of DCs significantly contributes to the observed cytotoxicity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have previously shown that CD 154 ( CD 40 ligand ) CD 40 molecular interactions are required both for the initiation and progression of MAIDS . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Proinflammatory cytokines and CD 40 ligand enhance cross presentation and cross priming capability of human dendritic cells internalizing apoptotic cancer cells . ^^^ Proinflammatory cytokines ( PC ) , CD 40 ligand ( CD40L ) and / or interferon gamma ( IFN gamma ) were found to markedly enhance the immunogenicity of TAA presented by DC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of CD 40 ligand expression in human T cells by biomaterials derived from left ventricular assist device surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of CD 40 and CD 40 ligand by the AT hook transcription factor AKNA . ^^^ Here we describe AKNA , a human AT hook protein that directly binds the A / T rich regulatory elements of the promoters of CD 40 and CD 40 ligand ( CD40L ) and coordinately regulates their expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
X linked hyper IgM syndrome : a report of the first case in Thailand with a confirmed mutation of CD 40 ligand gene . 10 linked hyper IgM ( XHIM ) syndrome is a rare congenital immunodeficiency disease caused by failure of B cell to isotype switch from IgM to other classes of immunoglobulins in response to infections . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD154 ( CD 40 ligand ) interaction blockade by the use of anti CD 154 monoclonal antibody ( mAb ) has shown efficacy in preventing allorejection in several models of organ and cell transplantation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 Ligand an anti apoptotic molecule in Hodgkin ' s disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of such immune responses can be mediated , in part , through the interaction of the T lymphocyte expressed molecule CD 40 ligand ( CD40L ) with its receptor on MP , CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Only myeloid DCs from the PP produce high levels of IL 10 upon stimulation with soluble CD 40 ligand ( ) trimer , or Staphylococcus aureus and IFN gamma . ^^^ In contrast , lymphoid and DN , but not myeloid DCs , produce IL 12p70 following microbial stimulation , whereas no DC subset produces IL 12p70 in response to CD 40 ligand trimer . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Vaccination against the intracellular pathogens Leishmania major and L . amazonensis by directing CD 40 ligand to macrophages . ^^^ CD 40 ligand ( CD40L ) is a potent inducer of interleukin 12 ( IL 12 ) production from macrophages and dendritic cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Platelet CD 40 ligand ( CD40L ) subcellular localization , regulation of expression , and inhibition by clopidogrel . ^^^ This study compares the subcellular localization and the regulation of expression of the platelet activation markers CD62P and CD 63 with CD 40 ligand ( CD40L ) on the surface of washed human platelets . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Growth inhibitory effects of CD 40 ligand ( CD 154 ) and its endogenous expression in human breast cancer . ^^^ Treatment with the soluble recombinant CD 40 ligand ( CD40L ) molecules gp 39 or CD40L trimer significantly reduced [ 3H ] thymidine uptake in BT 20 and T47D cells by up to 40 % , but did not affect the growth of CD 40 negative MCF 7 or ZR 75 1 cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , the surface expression of CD 28 or CD 40 ligand ( CD40L ) was not significantly changed after treatment with 20 m M NAC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cell stimulation of B cell proliferation during T B collaboration requires membrane bound stimulatory ligands , such as CD 40 ligand and the secretion of soluble cytokines , such as IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Maintained CD 40 and loss of polarised CD 40 ligand expression in oral squamous cell carcinoma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we assessed the effects of CD 40 stimulation using a murine agonistic CD 40 monoclonal antibody ( MoAb ) ( FGK 115 ) or a soluble recombinant murine CD 40 ligand ( srmCD40L ) in both lethally irradiated and nonirradiated BALB / c mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD 40 ligand ( CD 154 ) engagement is required but not sufficient for modulating MHC class 1 , ICAM 1 and Fas expression and proliferation of human non small cell lung tumors . ^^^ To determine the possible functional significance of CD 40 expression on human non small cell lung carcinomas and to assess the potential of CD 40 as a therapeutic target , 18 lung tumor cell lines were established from biopsy tissues and were monitored for phenotypic changes on the cell surface and alterations in tumor cell proliferation after the ligation of CD 40 with a trimeric fusion protein complex of CD 40 ligand ( CD40Lt ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To induce immune tolerance , a hamster monoclonal antibody specific for murine CD 40 ligand ( CD40L or CD 154 ) was injected with fVIII . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To begin to resolve these problems , the expression and modulation of the family of inhibitor of apoptosis proteins ( IAPs ) were investigated , with consideration given to the possibility that physiological stimuli , such as CD 40 ligand ( CD40L ) , available to B cells in the microenvironment , might modulate IAP expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Neonatal exposure to antigen induces a defective CD 40 ligand expression that undermines both IL 12 production by APC and IL 2 receptor up regulation on splenic T cells and perpetuates IFN gamma dependent T cell anergy . ^^^ Our findings indicate that CD 40 ligand expression on these splenic T cells is defective , leading to noneffective cooperation between T lymphocytes and APCs and a lack of IL 12 production . ^^^ More striking , this cellular system revealed a requirement for IL 2R expression for CD 40 ligand initiated , IL 12 driven progression of T cells into IFN gamma production . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) is important for T / B lymphocyte interaction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The positive proportion of cells expressing CD 40 ligand from CD4KO mice or CD4L mice was much lower than those from wild type mice . ^^^ There was no difference between CD4KO and CD4L mice in T cells expressing CD 40 ligand after stimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD28 / CD152 ( CTLA 4 ) CD80 / CD86 and CD 40 CD154 ( CD 40 ligand ) interactions are such receptor / ligand pairs that have been shown to be important in interactions between antigen presenting cells and T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using neutralizing antibody or cytokine inhibitors we provide evidence that enhancement of HGF production was due to additive effects of T cell membrane associated interleukin 1 ( IL 1 ) and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Proinflammatory signals , including LPS , IL 1 , TNF , and CD 40 ligand , induced FKN , as did IFN gamma , which had synergistic activity with TNF . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand expression in mycobacterium bovis BCG infection and its regulation by cytokines : a direct role of interleukin 12 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Coexpression of normal and mutated CD 40 ligand with deletion of a putative RNA lariat branchpoint sequence in 10 linked hyper IgM syndrome . ^^^ We describe a novel CD 40 ligand ( CD40L ) splicing mutation in a patient with 10 linked hyper IgM syndrome ( 10 HIM ) associated with alternate splicing of exon 3 , resulting in the expression of both full length and exon 3 skipped CD40L mRNA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation of DCs by CD 40 ligand further up regulated the expression of ELC ( 25 fold ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of CD 40 with CD 40 ligand ( CD40L ) transfected baby hamster kidney cells induces NF kappaB , as demonstrated by the activation of 1 kappaB kinase ( IKK ) , increased NF kappaB DNA binding , and p 65 nuclear translocation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Macrophages were stimulated in vitro with LPS , lipoteichoic acid , CD 40 ligand , or low molecular mass hyaluronic acid . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blockade of CD 40 ligand suppresses chronic experimental myasthenia gravis by down regulation of Th 1 differentiation and up regulation of CTLA 4 . ^^^ Th 1 type cells and costimulatory factors such as CD 40 ligand ( CD40L ) contribute to disease pathogenesis by producing proinflammatory cytokines and by activating autoreactive B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the PP , moreover , HOT augmented the IL 12p40 mRNA expression and spontaneous or CD 40 stimulated IL 12 production , and increased the number of CD4+ cells expressing CD 40 ligand , which is up regulated by IL 12 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Neal Nathanson of the National Institutes of Health ( NIH ) , who called HIV `` particularly insidious . ' ' Topics included envelope glycoproteins ; enhancing CTL responses ; soluble CD 40 ligand trimer and its effect on growth of antigen specific T cells ; responses of immunized monkeys to challenges with SHIV ; CD8+ T cell responses in vaccinated chimpanzees ; an anti HIV vaccine regimen using DNA and E 1 deleted adenoviral recombinant vaccines ; use of plasma alpha 2 macroglobulin ( A2M ) for enhanced antigen processing ; effects of a tandemly repeated HIV 1 V 3 peptide sequence ; intragastric immunization with listeria monocytogenes expressing HIV 1 gag protein ; and production of antigenic determinants of HIV envelope protein using plant viruses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antibodies to the other co stimulatory molecules CD 40 ligand , CD 80 ( B 7 1 ) , and CD 86 ( B 7 2 ) , although inhibitory , were less effective . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Bone marrow transplantation for CD 40 ligand deficiency : a single centre experience . ^^^ BACKGROUND : CD 40 ligand ( CD40L ) deficiency is a rare 10 linked immunodeficiency disorder leading to recurrent bacterial infection , with cryptosporidial enteritis and subsequent hepatic cirrhosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition to the selective induction of IL 12p40 in TNFalpha stimulated DCs , PGE ( 2 ) inhibited the production of IL 12p70 and IL 12p40 in DCs stimulated with LPS or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Role of CD 40 ligand signaling in defective type 1 cytokine response in human immunodeficiency virus infection . ^^^ This study investigated the possibility that perturbations in CD 40 ligand signaling are involved in this defect . ^^^ CD 40 ligand trimer ( CD40LT ) stimulated peripheral blood mononuclear cell ( PBMC ) production of IL 12 in response to Toxoplasma gondii and cytomegalovirus ( CMV ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 ligand , interferon gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts : correlation with acute rejection . ^^^ The purpose of the present study was to investigate the expression ( mRNA ) of CD 40 ligand ( CD40L ) , interferon gamma ( IFN gamma ) and Fas ligand ( FasL ) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD 154 ) and tumour necrosis factor related apoptosis inducing ligand ( Apo 2L ) in haematological malignancies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 activated PCL cells for vaccination were similarly generated ex vivo by co culture with CD 40 ligand transfectants . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we show that human CD 25 ( + ) CD 4 ( + ) Tr cells isolated from peripheral blood failed to proliferate and displayed reduced expression of CD 40 ligand ( CD40L ) , in response to T cell receptor mediated polyclonal activation , but strongly upregulated cytotoxic T lymphocyte associated antigen ( CTLA ) 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To determine whether CD 4 ( + ) CD 25 ( + ) cells regulate T cell responses to alloantigen and are critical for tolerance induction , murine CD 4 ( + ) T cells were tolerized to alloantigen via ex vivo CD 40 ligand ( CD40L ) / CD40 or CD28 / cytotoxic T lymphocyte associated antigen 4 / B7 blockade resulting in secondary mixed leukocyte reaction hyporesponsiveness and tolerance to alloantigen in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The costimulatory molecule CD 40 , expressed on antigen presenting cells , is thought to interact with CD 40 ligand ( CD40L ) expressed on activated CD 4 ( + ) or CD 8 ( + ) T cells to further drive interleukin 2 receptor ( IL 2R ) expression and antigen specific T cell expansion necessary for both class 2 and class 1 responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Distinct cytokine regulation by cholera toxin and type 2 heat labile toxins involves differential regulation of CD 40 ligand on CD 4 ( + ) T cells . ^^^ CT but not LT IIa or LT IIb significantly reduced the expression of CD 40 ligand ( CD40L ) on CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Autologous antileukemic immune response induced by chronic lymphocytic leukemia B cells expressing the CD 40 ligand and interleukin 2 transgenes . ^^^ To stimulate CLL specific immune responses , we sought to transfer the human CD 40 ligand ( hCD40L ) gene to B CLL cells , using an adenoviral vector , in order to upregulate costimulating factors on these cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand therapy of lymphoma patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using the same techniques , we were able to identify mutations in the CD 40 ligand gene in three families in which one of the members had clinical and biological phenotype compatible with 10 linked hyper IgM . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Donor monocytes are used to generate dendritic cells ( DCs ) in medium with autologous plasma , interleukin 4 , granulocyte macrophage colony stimulating factor , and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , IL 1 beta is identified as a new IL 12 inducing agent , acting conjointly with CD 40 ligand ( CD40L ) on human monocyte derived DC in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , attenuated expression of CD 40 and CD 40 ligand by stimulation in vitro was noted only in BN rats . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To stimulate NHL specific immune responses , we attempted to transfer the human CD 40 ligand ( hCD40L ) gene to B NHL cells and enhance their co stimulatory potential . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When , FL has only minor or short term effects , the anti tumor response can be significantly amplified by adding other cytokines , known to act at the T cell level , such as CD 40 ligand ( CD40L ) or 4 1BBL , a TNF family member . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Biophysical characterization of a soluble CD 40 ligand ( CD 154 ) coiled coil trimer : evidence of a reversible acid denatured molten globule . ^^^ The CD 40 ligand molecule is unique , consisting of a receptor binding domain anchored by an isoleucine zipper moiety . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 1 enhances T cell dependent antibody production through induction of CD 40 ligand and OX 40 on T cells . ^^^ We found that CD 40 ligand and OX 40 expression on T cells was affected by the mutation , and the reduced Ag specific B cell response in IL 1 ( / ) mice was recovered by the treatment with agonistic anti CD 40 mAb both in vitro and in vivo . ^^^ These observations indicate that IL 1 enhances T cell dependent Ab production by augmenting CD 40 ligand and OX 40 expression on T cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Additionally , we demonstrate that engagement of HDMEC CD 40 with its recombinant CD 40 ligand augments the induction of E selectin , but not of intercellular or vascular cell adhesion molecules on the surface of HDMEC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of antitumor immunity by transduction of CD 40 ligand gene and interferon gamma gene into lung cancer . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The TNF superfamily members LIGHT and CD 154 ( CD 40 ligand ) costimulate induction of dendritic cell maturation and elicit specific CTL activity . ^^^ However , LIGHT cooperates with CD 154 ( CD 40 ligand ) in DC maturation , with particular potentiation of allogeneic T cell proliferation and cytokine secretion of IL 12 , IL 6 , and TNF alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This reduction in CIITA expression occurs in human monocyte derived DCs and mouse bone marrow derived DCs , and is triggered by a variety of different maturation stimuli , including lipopolysaccharide , tumor necrosis factor alpha , CD 40 ligand , interferon alpha , and infection with Salmonella typhimurium or Sendai virus . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we show that CD 4 ( + ) T cells from CD4C / HIV 1 Tg mice , which develop a severe AIDS like disease , express low levels of CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Molecular identity of platelet CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Structure of CD 40 ligand in complex with the Fab fragment of a neutralizing humanized antibody . ^^^ BACKGROUND : CD 40 ligand ( CD40L or CD 154 ) , a member of the tumor necrosis factor ( TNF ) family , plays a critical role in both humoral and cellular immune responses and has been implicated in biological pathways involving epithelial cells , fibroblasts , and platelets . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transcript profiling of human dendritic cells maturation induced under defined culture conditions : comparison of the effects of tumour necrosis factor alpha , soluble CD 40 ligand trimer and interferon gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NF kappaB is involved in the regulation of CD 154 ( CD 40 ligand ) expression in primary human T cells . ^^^ Cognate interactions between CD 154 ( CD 40 ligand , CD40L ) on activated T cells and its receptor CD 40 on various antigen presenting cells are involved in thymus dependent humoral immune responses and multiple other cell mediated immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Overexpression of CD 40 ligand in murine epidermis results in chronic skin inflammation and systemic autoimmunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Signaling lymphocytic activation molecule is expressed on CD 40 ligand activated dendritic cells and directly augments production of inflammatory cytokines . ^^^ In this study , we present evidence that signaling lymphocytic activation molecule ( SLAM ) , a molecule first identified in activated T and B cells , is strongly up regulated in DC activated through CD 40 , as well as in response to inflammatory stimuli , including polyinosinic polycytidylic acid and LPS . mRNA encoding both membrane bound and soluble secreted isoforms of SLAM was detected in CD 40 ligand activated DC , comprising two of the four known SLAM isoforms . ^^^ Expression of membrane bound SLAM protein peaked at 12 h poststimulation with CD 40 ligand , gradually returning to baseline levels after 6 days . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 alone by either cross linking antibodies against CD 40 or a recombinant CD 40 ligand ( CD 154 ) was unable to induce the production of NO in BV 2 microglial cells . ^^^ Consistently , dominant negative mutants of p 65 ( Deltap 65 ) and C / EBPbeta ( DeltaC / EBPbeta ) inhibited the expression of iNOS in BV 2 microglial cells that were stimulated with the combination of IFN gamma and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Because CD 40 is also widely expressed in carcinomas , we studied whether CD 40 activation of these cells using soluble recombinant trimeric human CD 40 ligand ( srhCD40L ) can also induce T cell responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cytotoxic activity of human dendritic cells is differentially regulated by double stranded RNA and CD 40 ligand . ^^^ In this study , we investigate whether dsRNA and CD 40 ligand ( CD40L ) , that were characterized as potent inducers of DC maturation , could also stimulate or modulate DC cytotoxicity toward tumoral cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , we investigated the effect of N substituted benzamides on intact promoters for two genes central in immune regulation ; the CD 40 ligand ( CD40L ) and IL 2 promoters . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rat B cells are able to bind IL 13 and to proliferate when cultured with CD 40 ligand and IL 13 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we report that a monoclonal antibody ( mAb ) to TSA 1 , generated by immunizing a hamster with CD 40 ligand ( CD40L ) activated B cells , interferes with the function of FcgammaRIIB on splenic B cells and the B cell lymphoma cell line , M 12 , by binding to TSA on the same cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Production of this activity was restricted to DC and was not increased following DC activation by LPS or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A dual function DNA vaccine encoding carcinoembryonic antigen and CD 40 ligand trimer induces T cell mediated protective immunity against colon cancer in carcinoembryonic antigen transgenic mice . ^^^ A carcinoembryonic Ag ( CEA ) based DNA vaccine encoding both CEA and CD 40 ligand trimer achieved effective tumor protective immunity against murine colon carcinoma in CEA transgenic mice by activating both naive T cells and dendritic cells . ^^^ Taken together , these results suggest that a dual function DNA vaccine encoding CEA and CD 40 ligand trimer combined with tumor targeted IL 2 may be a promising strategy for the rational development of DNA based cancer vaccines for future clinical applications . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We showed that PPARgamma is highly expressed in immature human monocyte derived DC ( MDDC ) and that it may affect the immunostimulatory function of MDDC stimulated with lipopolysaccharide ( LPS ) or via CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Toll like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD 40 ligand to induce high amounts of IL 12 . ^^^ CD 40 ligand ( CD40L ) and CpG ODN synergized to activate PDC and to stimulate the production of IFN alpha and IL 12 including bioactive IL 12 p 70 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We describe the construction of a novel soluble dodecameric form of CD 154 ( CD 40 ligand ) that is more effective than trimeric tCD 154 in triggering B cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : Purified synovial fluid ( SF ) B cells were cultured in the presence of CD 40 ligand ( CD40L ) expressing fibroblasts and cytokines , activated T cells , or phorbol myristate acetate ( PMA ) / ionomycin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS AND RESULTS : Transcriptional profiling and reverse transcription polymerase chain reaction analysis revealed inducible expression of MMP 8 transcripts in CD 40 ligand stimulated mononuclear phagocytes . ^^^ Western blot analysis demonstrated that 3 atheroma associated cell types , namely , endothelial cells , smooth muscle cells , and mononuclear phagocytes , expressed MMP 8 in vitro upon stimulation with proinflammatory cytokines such as interleukin 1beta , tumor necrosis factor alpha , or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It comprises 35 kD ( p 35 ) and 40 kD ( p 40 ) polypeptides encoded by separate genes that are induced by a range of stimuli that include lipopolysaccharide ( LPS ) , DNA , and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of CD 40 ligand expression in systemic lupus erythematosus . ^^^ CD 40 ligand ( CD40L , CD 154 ) , a member of the tumor necrosis factor family of cell surface molecules , mediates these contact dependent signals delivered by CD 4 + T helper cells to CD 40 + target cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant CD 40 ligand therapy has significant antitumor effects on CD 40 positive ovarian tumor xenografts grown in SCID mice and demonstrates an augmented effect with cisplatin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 is the receptor for CD 40 ligand ( CD40L ) , a molecule predominantly expressed by activated CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Abnormal CD 40 ligand ( CD 154 ) expression in human immunodeficiency virus infected children . ^^^ The CD 40 ligand ( CD 154 ) , expressed primarily on activated CD 4 positive T cells , is a costimulatory molecule involved in B cell proliferation , germinal center formation , and immunoglobulin class switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Trapidil and anti CD 154 ( CD 40 ligand ) antibody inhibited the increase of procoagulant activity in the mixed lymphocyte reaction ; trapidil also suppressed the induction of tissue factor , monocyte chemoattractant protein 1 ( MCP 1 ) and matrix metalloproteinase 9 in the mixed lymphocyte reaction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Significant inhibition of established lung metastases required immunization with peptide pulsed DCs pretreated with CD 40 ligand that has been demonstrated to increase the T cell stimulatory activity of DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upregulation of CD 40 and CD 40 ligand ( CD 154 ) in patients with moderate hypercholesterolemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : CBA ( H2k ) mice received i . v . injections with donor splenic dendritic cells or B cells in the absence or presence of monoclonal antibody ( mAb ) specific for CD 40 ligand or CD80 / CD86 2 weeks before transplantation of a C57BL / 10 ( H2b ) heart . ^^^ However , when CD 40 pathway was blocked by anti CD 40 ligand mAb , i . v . injection of donor dendritic cells but not B cells induced indefinite graft survival ( MST > 100 and 20 days , respectively ) . ^^^ Mice treated with anti CD 40 ligand mAb alone rejected their grafts with a MST of 18 days . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we demonstrate that bone marrow derived dendritic cells ( DCs ) expressing the murine CD 40 ligand , when pulsed ex vivo by PC antigen , elicited significant titers of anti PC IgG in CD 4 deficient mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , rejection was little affected by CD 40 ligand deficiency . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dependence of murine obstructive airway disease on CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and lipopolysaccharide enhance the in vitro generation of melanoma reactive T cells . ^^^ We have developed an efficient and reproducible method for detecting tumor specific T cells by optimizing the activation of antigen presenting cells ( APC ) in peripheral blood mononuclear cells ( PBMC ) of metastatic melanoma patients with soluble trimeric CD 40 ligand ( CD40L ) or lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent work has documented overexpression of the potent immune mediator CD 40 and its counterpart CD 40 ligand ( CD40L ) in experimental and human atherosclerotic lesions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon terminal differentiation with LPS or CD 40 ligand , the acquisition of a mature surface phenotype was variably achieved among WAS patients , with increased expression of CD 80 , CD 86 and DC LAMP . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Efficient cross presentation required uptake of dead cells by immature DC and exposure to maturation stimuli , especially CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand promotes priming of fully potent antitumor CD 4 ( + ) T cells in draining lymph nodes in the presence of apoptotic tumor cells . ^^^ Here , we demonstrate that antitumor reactivity induced in regional lymph nodes ( LNs ) by s . c . injection of CD 40 ligand ( CD40L ) transduced tumor ( MCA 205 CD40L ) showed far superior therapeutic efficacy against established brain tumors of a weakly immunogenic fibrosarcoma , MCA 205 , when adoptively transferred . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Such proliferation depended on physical contact between CPSs and viable splenic adherent cells ( SAC ) and CD 40 ligand induced intracellular signal transduction . ^^^ Blockade of CD 40 ligand by relevant mAb down regulated the CPS induced anti inflammatory cytokine production and abolished the enhancement of fungus growth in cocultures of SAC and CD 4 ( + ) T lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To determine whether additional factors were involved in TCS induced IgE response , th e kinetic expression of CD 40 ligand ( CD40L ) , tumor necrosis factor alpha ( TNF alpha ) , and interleukin 13 ( IL 13 ) were measured by semi quantitative RT PCR in draining mesenteric lymph node ( MLN ) from TCS immunized mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A T cell specific enhancer of the human CD 40 ligand gene . ^^^ We observed that the human CD 40 ligand ( CD40L ) gene 5 ' flanking region conferred weak promoter activity in activated CD 4 T cells , suggesting that additional regions are required for optimal CD40L gene transcription . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cutting Edge : Ectopic expression of CD 40 ligand on B cells induces lupus like autoimmune disease . ^^^ CD 40 ligand ( CD40L ) is ectopically expressed on B cells in patients with systemic lupus erythematosus ( SLE ) and lupus prone BXSB mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Requirement for NK cells in CD 40 ligand mediated rejection of Philadelphia chromosome positive acute lymphoblastic leukemia cells . ^^^ We are studying the potential to elicit autologous antileukemic immune responses by introducing genes encoding immunomodulators ( CD 40 ligand ( CD40L ) , CD 80 , and GM CSF ) into leukemia cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The hyper IgM syndrome is a rare , inherited immune deficiency disorder resulting from defects in the CD 40 ligand / CD40 signaling pathway . 10 linked hyper IgM is caused by defects in the CD 40 ligand gene that prevent it from delivering an activation signal to antigen presenting cells via CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Identification of CD 40 ligand in Alzheimer ' s disease and in animal models of Alzheimer ' s disease and brain injury . ^^^ The immune and inflammatory mediator CD 40 ligand ( CD40L ) can augment the activation of cultured microglia by amyloid beta protein ( Abeta ) and promote neuron death . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We postulated that increased autoantibody production in ITP might be attributable to either increased or prolonged expression of CD 40 ligand ( CD40L , CD 154 ) in T or B lymphocytes , as has been previously observed in systemic lupus erythematosus ( SLE ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Moreover , innovative immunobiologicals ( PEG TNFRI , PEG TNF alpha antibody fragments , soluble TNFRI , CTLA 4 Ig , CD 40 ligand antibody , antibodies against IFN gamma , IL 6 , IL 12 , IL 15 , IL 18 , complements ) , inhibitors of TNF alpha translation ( peptides , anti sense constructs ) or TNF alpha synthesis ( targeting NF kappa B , p 38 MAP kinase , phosphodiesterase 4 , TNF alpha converting enzyme ) are forthcoming . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Enforced and prolonged CD 40 ligand expression triggers autoantibody production in vivo . ^^^ Through B lymphocyte activation with CD 154 transfected L 929 cells both in vitro and in vivo , our data reveal that enforced and prolonged expression of CD 40 ligand overcomes the tightly regulated mechanisms of B cell activation , triggering the production of autoantibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Estrogen increases CD 40 ligand expression in T cells from women with systemic lupus erythematosus . ^^^ OBJECTIVE : To examine the in vitro effects of estrogen on CD 40 ligand ( CD40L ) expression in peripheral blood T cells isolated from patients with systemic lupus erythematosus ( SLE ) and normal controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interrupting the interactions between T lymphocytes and other cells by blocking co stimulatory molecules , such as CD 40 ligand or CTLA 4 Ig , may interfere with the early steps of pathogenesis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 1 beta enhances CD 40 ligand mediated cytokine secretion by human dendritic cells ( DC ) : a mechanism for T cell independent DC activation . ^^^ CD 40 ligand ( CD40L ) is a membrane bound molecule expressed by activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The observed effect was related to the fact that MPL also acts directly on T cells , likely through their Toll like receptors , by increasing their intracellular calcium and up regulating their CD 40 ligand expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
There was very little induction of TNF alpha by ligation of CD 40 alone by either cross linking antibodies against CD 40 or recombinant CD 40 ligand ( CD 154 ) . ^^^ Consistently , a dominant negative mutant of p 65 ( Delta ( p 65 ) ) and that of C / EBPbeta ( DeltaC / EBPbeta ) inhibited the expression of TNF alpha in BV 2 microglial cells stimulated with the combination of IFN gamma and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Normal human B lymphocytes can be expanded in vitro in the presence of feeder cells expressing the CD 40 ligand CD 154 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand deficient T cells from 10 linked hyper IgM syndrome carriers have intrinsic priming capability . ^^^ Deficiency in CD 40 ligand ( CD40L ) expression is associated with impaired T cell immunity in mouse models and in humans . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transient blockade of CD 40 ligand dissociates pathogenic from protective mucosal immunity . ^^^ Here we show that induction by oral OVA of CTLs in wild type mice , and of diabetes in mice expressing OVA transgenically in pancreatic beta cells , can be prevented by transiently blocking the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When stimulated by either lipopolysaccharide or fibroblasts expressing CD 40 ligand ( CD40L ) transfectants , dendritic cells ( DCs ) generated in IFN beta and IL 3 secreted high levels of IL 6 , IL 8 , and tumor necrosis factor alpha but low levels of IL 12 in comparison with DCs generated in IL 4 and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 ligand ( CD 154 ) in B and T lymphocytes of Hodgkin disease : potential therapeutic significance . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The 10 linked form of the disease is a consequence of mutations in the CD 40 ligand ( CD40L ) gene that encodes a protein expressed primarily on activated CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Molecular profiles of allograft rejection following inhibition of CD 40 ligand costimulation differentiated by cluster analysis . ^^^ In a model of cardiac transplantation investigating immunosuppression by inhibition of CD 40 ligand costimulation , we have applied a combination of cluster algorithms and self organizing maps to analyze a panel of 60 candidate genes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Suppression of autoimmune arthritis in interleukin 1 deficient mice in which T cell activation is impaired due to low levels of CD 40 ligand and OX 40 expression on T cells . ^^^ Expression of T cell surface molecule CD 40 ligand ( CD40L ) and OX 40 was determined by multicolor flow cytometric analysis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
X linked hyper IgM syndrome associated with Cryptosporidium parvum and Cryptococcus neoformans infections : the first case with molecular diagnosis in Korea . 10 linked hyper IgM syndrome ( XHIM ) is a rare primary immunodeficiency disorder , caused by mutations of the gene encoding CD 40 ligand ( CD40L ; CD 154 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we demonstrate that infusion of an mAb against CD 40 ligand ( CD40L ) further increases the efficacy of LTbetaR Ig , leading to complete prevention of GVHD . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Adenovirus mediated CD 40 ligand gene engineered dendritic cells elicit enhanced CD 8 ( + ) cytotoxic T cell activation and antitumor immunity . ^^^ Thus , DCs engineered to express CD40L by adenovirus mediated CD 40 ligand gene transfer may offer a new strategy in production of DC cancer vaccines . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immature DC loaded with HIV 1 IIIB infected , autologous , apoptotic CD 8 ( ) cells and matured with CD 40 ligand induced gamma interferon production in autologous CD 8 ( + ) and CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Therapy did not reduce levels of the soluble platelet ( sP ) activation markers sP selectin and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 and CD 154 ( CD 40 ligand ) are surface molecules that are central to the cross talk between T cells and antigen presenting cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Resting B cells were shown to express significantly lower levels of rab 7 when compared to adherent macrophages or to ' immature ' or ' mature ' dendritic cells . rab 7 expression was up regulated by stimulation of B cells with lipopolysaccharide or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased concentrations of soluble CD 40 ligand , RANTES and GRO alpha in preeclampsia possible role of platelet activation . ^^^ We examined the concentrations of soluble CD 40 ligand ( sCD40L ) and the chemokines RANTES and GRO alpha in platelet free plasma ( PFP ) , and unstimulated and SFLLRN stimulated platelet rich plasma ( PRP ) , as well as in platelet pellets before stimulation using enzyme immunoassays . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cell activation and allosensitization after left ventricular assist device implantation is due to T cell activation and CD 40 ligand expression . ^^^ Sera from LVAD recipients and controls were tested for the presence of anti HLA antibodies , and for soluble CD 40 ligand . ^^^ This resulted in increased T cell expression of CD 40 ligand and subsequent B cell activation , which was reduced by inhibitors of T cell activation ( CsA or anti CD 25 mAb ) or by anti CD 40 ligand mAb . ^^^ LVAD recipients demonstrated higher frequencies of anti HLA antibodies and serum levels of soluble CD 40 ligand compared with heart failure controls . ^^^ The results indicate that exposure of human mononuclear cells to LVAD derived biomaterial leads to T cell dependent B cell activation via CD 40 CD 40 ligand interaction , and suggest that treatment with calcineurin inhibitors or monoclonal antibodies against either CD 25 or CD 40 ligand could be effective at preventing B cell hyperreactivity and allosensitization after LVAD implantation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This pattern of responsiveness is strongly modified by a surrogate antigen ( Ag ) but is not altered by CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cross linking surface Ig delays CD 40 ligand and IL 4 induced B cell Ig class switching and reveals evidence for independent regulation of B cell proliferation and differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This may be due , at least in part , to induction of Btk dependent Bcl 2 family members by anti Ig after CD 40 ligand treatment . ^^^ However , the susceptibility to Fas mediated apoptosis of B cell targets stimulated by CD 40 ligand alone was increased in the absence of Btk . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand functions non cell autonomously to promote deletion of self reactive thymocytes . ^^^ CD 40 ligand ( CD40L ) deficient mice have been shown to have a defect in negative selection of self reactive T cells during thymic development . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Essential role for CD 40 ligand interactions in T lymphocyte mediated modulation of the murine immune response to pneumococcal capsular polysaccharides . ^^^ We investigated whether CD 40 ligand ( CD40L ) plays a role in T lymphocyte mediated regulation of the immune response to caps PS , which are considered thymus independent Ags . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The most common form of hyper IgM syndrome is of 10 linked inheritance and caused by CD 40 ligand gene mutations . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Clast5 / Stra13 is highly expressed in unstimulated , resting B cells and is rapidly downregulated by a variety of stimuli that activate B cells , including CD 40 ligand , anti IgM antibodies , lipopolysaccharides and interleukin 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Demonstration of biological activity of CD 40 ligand ( CD 154 ) in the domestic cat . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased soluble and platelet associated CD 40 ligand in essential thrombocythemia and reactive thrombocytosis . ^^^ CD 40 ligand ( CD40L ) is expressed on activated CD 4 ( + ) T lymphocytes and at the activated platelet surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Association of CD 40 ligand expression on HTLV 1 infected T cells and maturation of dendritic cells . ^^^ The DC maturation is induced by cell to cell contact with CD4+ T cells expressing CD 40 ligand ( L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Most surprisingly , IL 18 causes high level IgE production when administered to normal mice by causing CD4+ T cells to produce IL 4 and to express CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 and CD 40 ligand among cell populations within rheumatoid synovial compartment . ^^^ Augmented and prolonged expression of CD 40 ligand ( CD40L ) was recently reported in lymphoid cells from human lupus patients , suggesting that CD40 / CD40L pathway was involved in the pathogenesis of systemic autoimmune diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recombinant CD 40 ligand administration does not decrease intensity of Pneumocystis carinii infection in scid mice . 10 linked Hyper IgM Syndrome ( HIM ) is a rare congenital immunodeficiency recently demonstrated to be caused by a mutation in the gene encoding CD 40 ligand . ^^^ In this study we undertook to investigate whether treatment of P . carinii infected scid mice with murine recombinant CD 40 ligand trimer ( muCD40L ) for 21 days would facilitate clearance of the organisms . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This may be because at lower doses of Ag ( 5 nM ) , naive CD 4 ( + ) T cells express CD 40 ligand and OX 40 , whereas at higher doses ( 50 nM ) , they express Fas ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand immunotherapy in cancer : an efficient approach . ^^^ This review will discuss current anti cancer immunotherapy ; interleukin 2 therapy , tumor vaccine secreting Granulocyte macrophage colony stimulating factor , dendritic cells fused with tumor cells , and CD 40 ligand immunotherapy . ^^^ Moreover , we introduce our two kinds of CD 40 ligand immuno genetherapy ; ( 1 ) oral CD 40 ligand gene therapy against lymphoma using attenuated Salmonella typhimurium ( published in BLOOD 2000 ) , ( 2 ) cancer vaccine transfected with CD 40 ligand ex vivo for neuroblastoma ( unpublished ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Systemic treatment with antibody to CD 40 ligand ( aCD40L ) can prevent autoimmunity and transplant rejection in several animal models and is currently under evaluation in clinical trials . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated soluble CD 40 ligand is related to the endothelial adhesion molecules in patients with acute coronary syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Addition of anti CD 40 ligand antibody abolished T cell induced activation of EC . ^^^ Our data suggest that contact mediated activation of EC by T cells , involving ligand : counter ligand interactions such as CD 40 : CD 40 ligand , may represent a novel pathogenic mechanism of progression in inflammatory diseases such as atherosclerosis or rheumatoid arthritis . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IFN alpha enhances CD 40 ligand mediated activation of immature monocyte derived dendritic cells . ^^^ Extending our earlier observation that type 1 IFN promote DC maturation , we report that these cytokines also enhance DC differentiation by augmenting CD 40 ligand ( CD40L ) induced cytokine secretion by MoDC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Suppression of IL 12 production by soluble CD 40 ligand : evidence for involvement of the p44 / 42 mitogen activated protein kinase pathway . ^^^ In this study , we show that an almost complete suppression of IL 12 production can also be observed after preincubation of monocytes with costimulatory cell surface molecules that bind to members of the TNFR superfamily ( CD 40 ligand , TNF related activation induced cytokine ( TRANCE ) ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antitumor immune responses derived from transgenic expression of CD 40 ligand in myeloma cells . ^^^ In the present study , we engineered a mouse myeloma cell line J 558 with a cloned CD 40 ligand ( CD40L ) gene . ^^^ We demonstrated that ( 1 ) the engineered J558 / CD40L tumor cells expressing the CD 40 ligand molecule lost their tumorigenicity in syngeneic mice , and ( 2 ) the inoculation of J558 / CD40L tumor cells further lead to the protective immunity against wild type J 558 tumors . ^^^ Our data indicate that the introduction of co stimulatory CD 40 ligand molecule will be useful as a new strategy of immunogene therapy against tumors . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Analysis of a CD 40 ligand dinucleotide microsatellite in multiple sclerosis . ^^^ Because the CD 40 ligand is known to be highly expressed on the peripheral blood mononuclear cells ( PBMC ) of multiple sclerosis ( MS ) patients , and because activated helper T cells expressing CD 40 ligand have been found in the brain sections of MS patients , but not in those of normal controls , the protein is believed to be involved in MS development . ^^^ We studied the influence of a polymorphic dinucleotide repeat marker located in the 3 ' untranslated region of the 10 linked gene encoding CD 40 ligand ( CD40LG ) on susceptibility to and disease severity in MS . ^^^ In addition , the effect of the polymorphism on CD 40 ligand mRNA expression was assessed using PBMC from 54 MS patients and 22 controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Full maturation of rat BMDC ( day 12 ) with an almost uniform expression of B 7 was achieved by subsequent subculture and further stimulation with rat tumour necrosis factor alpha ( TNF alpha ) , lipopolysaccharide ( LPS ) or soluble CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DC generated from bone marrow precursors by granulocyte macrophage colony stimulating factor ( GM CSF ) expressed m Langerin , but this was shut down during maturation with CD 40 ligand or lipopolysaccharide . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of tissue factor expression in human endothelial cells by CD 40 ligand is mediated via activator protein 1 , nuclear factor kappa B , and Egr 1 . ^^^ However , the molecular mechanisms of tissue factor gene expression triggered by CD 40 ligand ( CD40L ) in this cell type remain unknown . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
As CLL cells retain the capacity to respond to CD 40 ligand ( CD40L ) and as CD 4 ( + ) T cells are always present in involved tissues , we asked whether malignant CLL cells might produce T cell attracting chemokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased expression of CD 40 ligand on activated T cells of patients with colon cancer . ^^^ In contrast , in vitro stimulated T cells of patients with colon cancer had a significantly increased expression of CD 40 ligand ( CD40L , CD 154 ) compared with activated T cells of the control group ; increased CD40L expression was also found in the CD 4 ( + ) and CD 8 ( + ) T cell subpopulations . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To examine the effects of CD 40 ligation on cell growth recombinant soluble CD 40 ligand was used to stimulate EOC cell lines and growth was measured by MMT assays . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we further examined the IL 12 / IL 12R axis by investigating the expression of IL 12R and CD 40 ligand ( CD40L ) in peripheral blood mononuclear cells ( PBMC ) of 18 HAM / TSP patients , and comparing the levels with those in 25 patients with other neurological disorders , including 4 anti HTLV 1 seropositive carriers as controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we have established a culture system to generate these APCs under clinically applicable conditions using guanylic acid grade soluble trimeric CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Below a normally suicidal threshold number of cells , low picomolar quantities of exogenous CD 40 ligand ( CD40L / CD154 ) were found to sustain the clone without the discernible shift in phenotype that accompanies high CD40L encounter . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , CD 40 ligand was essential for induction of IFN gamma in the spleen and LP , but not in the liver and lung , while the IL 4 response required CD 40 ligand only in the spleen . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligation by CD 40 ligand ( + ) CD 4 ( + ) T cells has been claimed to be involved in inflammatory responses in human skin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : CD 154 ( CD 40 ligand ) monoclonal antibody prevents allograft rejection in rodents and monkeys . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Fully activated T helper cells are competent to deliver help to B cells by secreting cytokines ( e . g . interleukin ( IL ) 4 ) or up regulating CD 40 ligand for proliferation and differentiation of B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
An increase in mRNA levels for TNF and Tnfrsf 1 in the bile ducts of Tnfsf 5 / ( CD 40 ligand or CD 154 knockout ) mice developing cholangitis following infection by Cryptosporidium parvum ( CP ) is accompanied by staining for TNFalpha in areas of inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Clustering of CD 40 ligand is required to form a functional contact with CD 40 . ^^^ We show that clustering of the CD 40 receptor depends on reciprocal clustering of the CD 40 ligand ( gp 39 , CD 154 ) . ^^^ Clustering of the CD 40 ligand is mediated by an association of the ligand with p 53 , a translocation of acid sphingomyelinase ( ASM ) to the cell membrane , an activation of the ASM , and a formation of ceramide . ^^^ Ceramide appears to modify preexisting sphingolipid rich membrane microdomains to fuse and form ceramide enriched signaling platforms that serve to cluster CD 40 ligand . ^^^ Genetic deficiency of p 53 or ASM or disruption of ceramide enriched membrane domains prevents clustering of CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) does not stimulate proliferation of vascular smooth muscle cells . ^^^ The present study investigates the effects of CD 40 ligand ( CD40L ) on mitogenic signalling , proliferation , and migration of cultured bovine coronary artery smooth muscle cells ( SMC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Besides their unique localization , they were characterized by the production of interferon ( IFN ) gamma , lack of the pore forming enzyme perforin , and expression of CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We hypothesized that CD 40 ligand ( CD40L ) and TNF alpha , which differ in their capacity to condition DC , would also differ in their capacity to activate NF kappaB . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Secretion of IgG and IgA was efficiently induced in cultures from purified B cells stimulated with the combination of MID or MID Sepharose and IL 4 , IL 10 , and soluble CD 40 ligand , suggesting that Th 2 derived cytokines were required for optimal plasma cell generation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased CD 4 and CCR 5 expression and human immunodeficiency virus type 1 entry in CD 40 ligand stimulated macrophages . ^^^ The effects of a soluble trimeric CD 40 ligand ( CD40L ) agonist on the expression of CD 4 and CCR 5 and on human immunodeficiency virus ( HIV ) type 1 entry into and replication in human macrophages were investigated . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The Ramos is protected from apoptosis by the crosslinking of sIgM and the calcium ionophore by the ligation of CD 40 with anti CD 40 monoclonal antibodies ( mAb ) or soluble CD 40 ligand ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have constructed and tested five recombinant adenoviruses ( Ads ) that express a variety of immunomodulators , including CD 40 ligand ( CD40L ) , a potent costimulator of several components of the immune system . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Endogenous Act 1 is recruited to the CD 40 receptor in human intestinal ( HT 29 ) and cervical ( HeLa ) epithelial cells upon stimulation with CD 40 ligand , indicating that Act 1 is involved in this signaling pathway . ^^^ Furthermore , transfection of Act 1 into C33A cervical epithelial cells , which do not express it , renders these cells sensitive to CD 40 ligand induced NF kappa B activation and protects them from CD 40 ligand induced apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However short term treatment with murine cytotoxic T lymphocyte associated antigen 4 ( muCTLA 41g ) and / or CD 40 ligand ( CD 154 ) monoclonal antibodies ( anti CD40L mAbs ) was unable to induce indefinite allograft survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transgenic expression of CD 40 ligand produces an in vivo antitumor immune response against both CD 40 ( + ) and CD 40 ( ) plasmacytoma cells . ^^^ Expression of CD 40 ligand ( CD40L ) proximate to the MM cells might serve this purpose , either by increasing their capacity to present self antigens by activation through their CD 40 receptor or by the recruitment of professional antigen presenting cells ( APCs ) able to take up and present tumor associated antigens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show here that CD 40 stimulation using either a soluble recombinant human CD 40 ligand ( srhCD40L ) or anti CD 40 monoclonal antibody resulted in apoptosis of human Burkitt lymphoma cell lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cardiopulmonary bypass induces release of soluble CD 40 ligand . ^^^ It is known that CD 40 ligand ( CD40L ) exists in platelets , that a soluble form of this protein ( sCD40L ) is released on platelet activation , that platelets are the primary source of sCD40L in blood , and that sCD40L is involved in thrombosis and inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , compound L 685 818 ( tacrolimus analog ) and CD 40 ligand ( CD40L ) , provided protection against lethal infection . ^^^ While mycophenolate did not exacerbate the infection , our data suggest potential therapeutic applications for L 685 818 and CD 40 ligand in trypanosomiasis . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Importantly , diltiazem induced DCs have an impaired responsiveness to lipopolysaccharide and CD 40 ligand because they produce decreased levels of IL 12 and reveal a reduced ability to stimulate alloreactive T cell responses as well as in inducing interferon gamma producing Th 1 cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 12 ) or an adenovirus coding for the CD 40 ligand have shown an intense antitumor effect against experimental colorectal cancer . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Irradiation up regulates CD 80 expression through induction of tumour necrosis factor alpha and CD 40 ligand expression on B lymphoma cells . ^^^ Irradiation of A 20 HL cells also up regulated the expression of tumour necrosis factor alpha ( TNF alpha ) and CD 40 ligand ( CD40L ) , and the amount of immunoprecipitable TNF alpha and CD40L in cell lysates . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) , as a single agent , has been shown to be able to generate DCs from PB monocytes , while numerous other reports have also demonstrated its role as a potent maturation factor . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD 154 ) does not contribute to lymphocyte mediated inhibition of virulent Mycobacterium tuberculosis within human monocytes . ^^^ Nevertheless , soluble CD 40 ligand ( CD40L ; also designated CD 154 ) had no effect on the intracellular growth of the organism . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of MHC class 2 , CD 54 , CD 80 and CD 86 by prostatic dendritic cells was not up regulated after castration and freshly isolated rhFL induced prostate cells were unable to prime allogeneicT cells unless they were activated by culture either on plastic or with recombinant soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of locally administered anti CD 154 ( CD 40 ligand ) monoclonal antibody on survival of allogeneic corneal transplants . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and CTLA 4 are reciprocally regulated in the Th 1 cell proliferative response sustained by CD 8 ( + ) dendritic cells . ^^^ When exposed to CD 8 ( + ) DCs in the absence of CD 40 activation , the Th 1 clone expressed low levels of CD 40 ligand and high levels of surface CTLA 4 . ^^^ Neutralization of CTLA 4 during the DC / T cell coculture resulted in increased CD 40 ligand expression and proliferation of T cells . ^^^ In addition , they suggest that reciprocal regulation of CD 40 ligand and CTLA 4 expression occurs in Th 1 cells exposed to CD 8 ( + ) DCs . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti lymphocyte function associated antigen 1 monoclonal antibody inhibits CD 40 ligand independent immune responses and prevents chronic vasculopathy in CD 40 ligand deficient mice . ^^^ BACKGROUND : Blockade of CD 40 ligand ( CD40L ; CD 154 , gp 39 ) is a potential treatment for autoimmune disease and allograft rejection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To explore the regulatory defects underlying the overexpression of CD 40 ligand ( CD40L , CD 154 ) in human lupus we studied the effects of cyclosporin A ( CsA ) , which blocks Ca2+ / calcineurin dependent CD40L gene expression , on peripheral blood derived T cells and monocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) , a glycoprotein involved in B cell proliferation , antigen presenting cell activation , and Ig class switching , is important in the immune response to infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
MUC1 tumor cell line was primarily mediated by CD 4 ( + ) T cells , and required Fas ligand , lymphotoxin alpha , CD 40 , CD 40 ligand and CD 28 , but not perforin , gammadelta T cells , IL 4 , IL 10 , IL 12 or tumor necrosis factor receptor 1 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Costimulation with PGE 2 enhanced the expression of the CCL19 / CCL21 receptor CCR 7 on the cell surface of MoDCs when they were matured with soluble CD 40 ligand or proinflammatory cytokines , but did not affect CCR 7 expression of polyI : C stimulated MoDCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Characterization of soluble CD 40 ligand released from human activated platelets . ^^^ We report here that soluble CD 40 ligand ( sCD40L ) is released from human platelets when activated with collagen or thrombin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effects of orally active glycoprotein IIb / IIIa antagonists on platelet CD 40 ligand ( CD 154 ) expression and platelet heteroaggregate formation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we show that CD 40 ligand , IL 2 , and IL 10 together drive this transition of GC B cells to memory phenotype in vitro , up regulating memory B cell markers , chemotactic responses to CXC ligand ( CXCL ) 12 , CXCL 13 , and CCL 19 , and expression of adhesion receptors L selectin , alpha ( 4 ) beta ( 7 ) , and CLA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that B cells stimulated with CD 40 ligand and IL 4 ( CD 40 B cells ) can be efficiently transduced with retroviral vectors encoding a model Ag , CMV tegument protein pp 65 gene , and maintain high levels of costimulatory molecules after gene transfer . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Efficient gene transfer of CD 40 ligand into primary B CLL cells using recombinant adeno associated virus ( rAAV ) vectors . ^^^ Using an optimized adenovirus free packaging system , recombinant adeno associated virus ( rAAV ) vectors coding for the enhanced green fluorescent protein ( AAV / EGFP ) and CD 40 ligand ( AAV / CD40L ) were packaged and highly purified resulting in genomic titers up to 3 10 10 ( 11 ) / mL . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Protection against spontaneous apoptosis could also be induced by coculturing the CLL B cells with normal dendritic cells ( DCs ) or with a CD 40 ligand ( CD 154 ) expressing fibroblast cell line . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Polyclonal B cell activators ( anti IgM and recombinant CD 40 ligand trimer ) showed minimal ability to independently induce Ly 6C expression on B cells but did enhance the ability of IFNs to induce expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Platelet CD 40 ligand interaction with melanoma cell and monocyte CD 40 enhances cellular procoagulant activity . ^^^ Activated platelets express and release CD 40 ligand ( CD40L ) , which induces endothelial TF expression by ligation to CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The half life of the CD 4 ( ) , but not CD 4 ( + ) DC in vitro was extremely short but cells could be rescued from death by CD 40 ligand , IL 3 , or GM CSF . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 2 receptor blockade inhibits late , but not early , IFN gamma and CD 40 ligand expression in human T cells : disruption of both IL 12 dependent and independent pathways of IFN gamma production . mAbs directed against the alpha chain ( Tac / CD25 ) of the IL 2R are an emerging therapy in both transplantation and autoimmune disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Microbial pathogens could 1 ) release molecules that bind Toll like receptors , and / or 2 ) stimulate microbe specific T cells to express CD 40 ligand , thereby licensing APCs that bear both microbial and autoantigens to break tolerance . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These observations may have important implications for the mechanism of action of agonistic anti CD 40 antibodies , which have been used as an adjuvant in models of infection and experimental tumours and the primary immunodeficiency Hyper IgM syndrome caused by deficiency of CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the present study , we analysed 27 B CLL peripheral blood samples by metaphase cytogenetics after CD 40 ligand ( CD40L ) induced cell cycle stimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Instead , commitment to apoptosis can be rapidly controlled by T cells via CD 40 ligand and TNF alpha , downstream of the pRb regulated restriction point of the cell cycle , but prior to mitochondrial cytochrome c release . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cross linking of TCR by MHC class 2 bound antigen activates T ( h ) cells , resulting in their up regulation of CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunoglobulin ( Ig ) class switch DNA recombination ( CSR ) is thought to be highly dependent upon engagement of CD 40 on B cells by CD 40 ligand on T cells . ^^^ We show here that dendritic cells up regulate BLyS and APRIL upon exposure to interferon alpha , interferon gamma or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We observed that ATPgammaS potentiated the IL 12p40 release induced by TNF alpha , but also by lipopolysaccharides ( LPS ) and soluble CD 40 ligand ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human lung myofibroblasts as effectors of the inflammatory process : the common receptor gamma chain is induced by Th 2 cytokines , and CD 40 ligand is induced by lipopolysaccharide , thrombin and TNF alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The biological outcome of CD 40 signaling is dependent on the duration of CD 40 ligand expression : reciprocal regulation by interleukin ( IL ) 4 and IL 12 . ^^^ CD 40 ligand ( CD 154 ) expression on activated T cells can be separated into an early TCR dependent phase , which occurs between 0 and 24 h after activation , and a later extended phase , which occurs after 24 h and is reciprocally regulated by the cytokines IL 4 and IL 12 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Although new molecules continue to be discovered , the functions of B 7 1 ( CD 80 ) , B 7 2 ( CD 86 ) , CD 28 , cytotoxic T lymphocyte antigen 4 ( CTLA 4 ) , inducible costimulator ( ICOS ) , programmed death 1 ( PD 1 ) , OX 40 ( CD 134 ) and CD 40 ligand ( CD40L , CD 154 ) are now sufficiently understood that immunologists are targeting them to manipulate T cells to slow the progression of autoimmune diseases or treat tumours through the increase in T cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We demonstrated that treatment of human gingival fibroblast ( HGF ) with human leukocyte elastase ( HLE ) , a neutrophil serine protease , down regulated the expression of CD 40 and binding to the CD 40 ligand ( CD40L ) using flow cytometry . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Because monocyte derived DC 1 produce interleukin 12 ( IL 12 ) when stimulated with CD 40 ligand ( CD40L ) , we hypothesized that similar results would be obtained with DC 1 mobilized by Flt3L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we investigated CD 40 ligand ( CD40L ) regulation by cyclic AMP ( cAMP ) and prostaglandin E ( 2 ) ( PGE ( 2 ) ) and observed differential effects depending upon the cell subset and mode of activation . cAMP inhibited CD 3 induced CD40L in both nai ; ve and memory subsets , although greater inhibition was observed in memory cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
During this contact , several surface molecules are implicated in contact mediated cytokine production , including CD 40 ligand , CD11b / c , and CD 69 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : Normal and malignant human urothelial cells were cultured with soluble TNF family agonists ( CD 40 ligand [ CD40L ] , TNF alpha , anti Fas antibody , or cocultured with mouse fibroblasts stably transfected with plasmids that caused the cells to constitutively express CD40L or CD 32 ; cell proliferation was estimated by an [ ( 3 ) H ] thymidine incorporation assay , and apoptosis was determined by Annexin 5 staining and by a DNA fragmentation assay . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In both cases , secretion of high levels of cytokine requires a second signal from T cells , which can be replaced by CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The immunological and infectious features observed in patients result from impaired NF kappaB signalling , including cellular response to LPS , IL 1beta , IL 18 , TNF alpha , Tlr 2 and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We report that monocyte derived , CD1a+ immature DCs ( iDCs ) significantly inhibit the growth of breast tumor cells in coculture and transwell experiments in the presence of soluble CD 40 ligand ( sCD40L ) , LPS or both . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In particular , ex vivo culture of ALL cells with CD 40 ligand , incubation of AML cells with granulocyte macrophage colony stimulating factor and interleukin 4 ( GM CSF / IL 4 ) and lentiviral transduction of ALL and AML cells for expression of immunomodulators ( CD 80 and GM CSF ) are current approaches under investigation for the development of autologous acute leukemia cell vaccines . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand stimulated B cell precursor leukemic cells elicit interferon gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia . ^^^ Childhood B cell precursor acute lymphoblastic leukemia ( BCP ALL ) cells , collected from bone marrow ( BM ) at diagnosis , were cultured , after thawing , on allogeneic human bone marrow stroma ( HBMS ) for 48 h in the presence of a soluble trimeric CD 40 ligand ( stCD40L ) molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
H 2 mismatched transplantation with repetitive cell infusions and CD 40 ligand antibody infusions without myeloablation . ^^^ We have established a tolerization model , using 1 Gy irradiation , which reduces stem cell capacity to < 10 % of control while causing minimal myelosuppression , donor antigen pre exposure ( spleen cells ) , CD 40 ligand antibody blockade and high levels of marrow ( 40 10 106 cells ) , which allows for stable long term multilineage engraftment in H 2 mismatched murine marrow transplants . ^^^ We now show that the establishment of ' microchimaerism ' ( 0 . 5 3 . 8 % ) sets the stage for macrochimaerism , with subsequent marrow infusions in H 2 mismatched mice with CD 40 ligand blockade only . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : In many situations , anti CD 154 ( CD 40 ligand ) monoclonal antibody ( mAb ) treatment is very potent in producing allograft tolerance . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Importantly , viral replication is triggered upon activation of IPC with CD 40 ligand , a signal physiologically delivered by CD 4 T cells . ^^^ Nevertheless , IPC efficiently transmit HIV 1 to CD 4 T cells , and such transmission is also augmented by CD 40 ligand activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The effect of IL 4 , IL 10 , and IL 13 , alone or in combination , on Ig isotype switching by highly purified naive human CD 40 ligand ( CD40L ) activated B cells was measured against division number over various harvest times . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Enhanced interleukin 12 and CD 40 ligand activities but reduced Staphylococcus aureus Cowan 1 induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis . ^^^ Whole blood cell cultures from schistosomiasis patients were stimulated with a variety of T cell dependent and T cell independent stimuli to determine whether the defect in type 1 cytokine expression observed following helminth infection is associated with alterations in interleukin 12 ( IL 12 ) or CD 40 ligand ( CD40L ) responsiveness . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Szary syndrome patients demonstrate a defect in dendritic cell populations : effects of CD 40 ligand and treatment with GM CSF on dendritic cell numbers and the production of cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased production of pro inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL 1 and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation of CD 40 by CD 40 ligand is known to increase COX 2 and IL 8 expression in endometrial fibroblasts . ^^^ The likely source of CD 40 ligand in the uterus is platelets . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Monocyte chemoattractant protein 1 selectively inhibits the acquisition of CD 40 ligand dependent IL 12 producing capacity of monocyte derived dendritic cells and modulates Th 1 immune response . ^^^ We found that DCs generated in the presence of MCP 1 displayed a markedly reduced production of IL 12 in response to CD 40 ligand but not in response to Staphylococcus aureus stimulation in the presence or absence of IFN gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we examined the contribution of the major costimulatory molecules , CD 40 ligand ( CD40L ) , CD 80 , and CD 86 , in the priming of CD 8 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
One such signal is likely to be CD 40 ligand ( CD40L ) , as IKK2dn blocked CD40L but not lipopolysaccharide ( LPS ) induced NF kappaB activation , cytokine production , and up regulation of costimulatory molecules and HLA DR in DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 34 ( + ) derived int DCs mature in response to lipopolysaccharide ( LPS ) plus CD 40 ligand ( L ) and become capable of CCL21 / CCL19 mediated chemotaxis and naive T cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Role of CD 40 ligand in amyloidosis in transgenic Alzheimer ' s mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cells freshly stimulated with Epstein Barr virus were also transducible by HIV vectors ( 24 % + / 9 % ) , but B cells activated with CD 40 ligand and cytokines resisted transduction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Apoptosis induced by Pseudomonas aeruginosa in antigen presenting cells is diminished by genetic modification with CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Of the TNFSF molecules , CD 40 ligand ( CD40L , also called CD 154 or TNFSF 5 ) is the most crucial molecule for activating antigen presenting cells ( APCs ) and thereby initiating the immune response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Surprisingly , when other maturation stimuli were used , we observed that CsA significantly inhibited maturation induced by lipopolysaccharides but not by polyribocytidylic acid or CD 40 ligand , as assessed by DC phenotype and functions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This strategy decreased expression of CD 40 and the CD 40 ligand in the atherosclerotic plaque and normalized the increased chemokine ( RANTES and MCP 1 ) and cytokine ( tumor necrosis factor alpha , interleukin 6 , interleukin 1beta , and transforming growth factor beta ( 1 ) ) gene expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vivo class switching CLL B cells down regulate switch circles and circle transcripts in vitro unless exposed to exogenous CD 40 ligand and IL 4 . ^^^ In addition , CLL B cells that do not class switch in vivo activate the CSR machinery and secrete IgG , IgA , or IgE upon in vitro exposure to CD 40 ligand and IL 4 . ^^^ They also suggest that this process is elicited by external stimuli , including CD 40 ligand and IL 4 , provided by bystander immune cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Significance of chemokines and soluble CD 40 ligand in patients with autoimmune thrombocytopenic purpura . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of CD 40 ligand on the course of murine histoplasmosis . ^^^ CD 40 ligand CD 40 ligation is important in the development of T cell mediated immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of CD11c ( + ) B 220 ( + ) pDCs with oligodeoxynucleotides containing certain CpG motifs plus CD 40 ligand plus GM CSF led to increased MHC class 2 , CD 80 , CD 86 , and CD8alpha expression levels , to a switch in chemokine receptor expression that affected their migration , to IFN alpha and IL 12 secretion , and to the acquisition of priming capacities for both CD 4 ( + ) and CD 8 ( + ) OVA specific TCR transgenic naive T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We compared the standard maturation stimulus , autologous monocyte conditioned medium ( MCM ) , with a synthetic double stranded RNA ( poly 1 : C ) , soluble CD 40 ligand trimer , and a defined cocktail of cytokines ( TNF alpha , IL 1 beta , IL 6 ) and PGE ( 2 ) to promote mature phenotype and function in human monocyte derived DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Three different stimuli , CpG DNA plus CD 40 ligand , interleukin 3 ( IL 3 ) , and herpes simplex virus , stimulate plasmacytoid pre DCs to differentiate into DCs that induce distinct types of T helper cells , i . e . , Th 1 , Th 2 , and IFN gamma and IL 10 producing T cells , respectively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human DC subsets with the property of suppressing an antigen specific T cell response include plasmacytoid DCs , which are either in an immature state or in a mature state induced by CD 40 ligand stimulation , and monocyte derived DCs , which are either in an immature state or have had their state modulated by treatment with IL 10 or CD 8 ( + ) CD 28 ( ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To determine whether Fas mediated apoptosis of BL cells is reduced in the context of antigen to which the tumor IgM binds , we stimulated HBL 3 cells with CD 40 ligand ( CD40L , CD 154 ) in the presence and absence of human erythrocytes expressing the `` 1 ' ' antigen , and measured Fas mediated apoptosis upon exposure to an agonistic anti Fas antibody . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
One study using CEA peptides and CEA RNA found that optimal T cell presentation occurs when peptides are loaded after maturation with CD 40 ligand and when RNA is transfected before maturation with CD 40 ligand [ 53 ] . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand ( CD40L ) is the natural ligand for CD 40 ; it is expressed primarily on the surface of activated T lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activating monocytes with soluble CD 40 ligand ( CD40L ) led to accelerated differentiation toward mature CD 83 ( + ) DCs with up regulated human leukocyte antigen DR , costimulatory molecules and CD 116 ( GM CSF receptor ) , and down regulation of molecules involved in antigen capture . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Efficient T cell priming by GM CSF and CD 40 ligand double transduced C 26 murine colon carcinoma is not sufficient to cure metastases in a therapeutic setting . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The re expressed CD 28 was functional and restored the ability of CD 4 ( + ) CD 28 ( null ) T cells to express CD 25 and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We therefore tested human gene expression of Interleukin ( IL ) 2 , 4 and 10 , interferon gamma ( IFNgamma ) ; transforming growth factor beta 1 ( TGFbeta 1 ) ; and CD 40 ligand ( CD40L ) in splenic extracts of SCID mice after engraftment of PBLs from two persons ( direct MLR ) or one person plus allopeptides ( indirect MLR ) in the presence or absence of cyclosporin A ( CsA ) or FK 506 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of human glioma cell growth by a soluble recombinant human CD 40 ligand ] . ^^^ CD 40 stimulated by its antibody or CD 40 ligand previously had been demonstrated to induce activation induced cell death in ovarian carcinoma cell lines , colon carcinoma cell lines , breast carcinoma cell lines , and lung carcinoma cell lines and to inhibit their growth in vivo . ^^^ This study was designed to assess the effects of a recombinant soluble human CD 40 ligand ( srhCD40L ) on human glioma cell lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Delineation of a novel pathway that regulates CD 154 ( CD 40 ligand ) expression . ^^^ The expression of CD 154 ( CD 40 ligand ) by activated T lymphocytes plays a central role in humoral and cellular immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In response to stimulation with CD 40 ligand alone or with IL 10 , both IgM expressing and Ig isotype switched memory B cells entered their first division 20 30 h earlier than did naive B cells . ^^^ Proliferation of CD 40 ligand stimulated naive B cells as well as both CD 38 ( + ) and CD 38 ( ) cells present in cultures of memory B cells was increased by IL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A complex containing polypyrimidine tract binding protein is involved in regulating the stability of CD 40 ligand ( CD 154 ) mRNA . ^^^ CD 40 ligand ( CD 154 ) expression has been shown to be regulated , in part , at the posttranscriptional level by a pathway of `` regulated instability ' ' of mRNA decay throughout a time course of T cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Should soluble CD 40 ligand be measured from serum or plasma samples . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Circulating soluble CD 40 ligand in patients with eosinophilic fasciitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Flow cytometry confirmed normal binding properties of the mutant to CD 40 ligand . ^^^ Stimulation with CD 40 ligand resulted in clustering of wild type CD 40 , while the chimeric CD40 / 45 receptor failed to cluster . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate the mechanism underlying this dual process , we followed the fate of human B cells activated in vitro with the T cell derived signals CD 40 ligand ( CD40L ) , IL 2 , and IL 10 using CFSE to monitor cell division . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Raised serum levels of soluble CD 40 ligand in patients with familial hypercholesterolemia : downregulatory effect of statin therapy . ^^^ OBJECTIVES : In the present study , we investigated the effects of statins on serum levels of soluble CD 40 ligand ( sCD40L ) in patients with familial hypercholesterolemia ( FH ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In macrophages , VEGF has been shown to be upregulated by the inflammatory mediator lipopolysaccharide ( LPS ) and by engagement of CD 40 by CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Plasma levels of soluble CD 40 ligand are elevated in inflammatory bowel diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study shows that human blood monocyte derived dendritic cells loaded with liposome complexed HIV 1 proteins and matured with CD 40 ligand can prime CD 8 ( + ) T cells to HIV 1 in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of monocyte CD 40 by the T cell derived CD 40 ligand can trigger the production of various mediators , the transcription and activation of enzymes , and the upregulation of costimulatory molecules involved in the pathogenesis of sepsis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 ligand on CD4+ T cells and platelets correlated to the coronary artery lesion and disease progress in Kawasaki disease . ^^^ CD 40 ligand ( CD40L ) has been implicated in certain types of vasculitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We applied this approach to investigate selection at two genes carrying common variants implicated in resistance to malaria : G6PD and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand in pathogenesis of autoimmune ovarian disease of day 3 thymectomized mice : implication for CD 40 ligand antibody therapy . ^^^ The blockade of CD 40 ligand ( CD40L ) is effective in autoimmune disease prevention . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have identified a CD 40 regulated gene , Clast6 / E3 / LAPTM5 , by subtraction of cDNAs derived from resting and CD 40 ligand treated B cells . ^^^ Clast6 / E3 / LAPTM5 is abundantly expressed in resting mature B cells but is rapidly and transiently repressed by treatment with CD 40 ligand , a T helper signal that induces B cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immuno gene therapy with adenoviruses expressing fms like tyrosine kinase 3 ligand and CD 40 ligand for mouse hepatoma cells in vivo . ^^^ CD 40 ligand ( CD40L , CD 154 ) and fms like tyrosine kinase 3 ligand ( Flt3L ) are recently identified cytokines , which have been demonstrated to stimulate antitumor immunity in several cancer models . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To characterize the nature of the immune response , we measured antigen specific production of T helper cell cytokines and CD 40 ligand by flow cytometry and immunoglobulin subclass levels in peripheral blood . ^^^ We found that normal control subjects made Th 1 response to NTHi with distinct CD 40 ligand production . ^^^ In contrast , subjects with bronchiectasis had predominant production of Th 2 cytokines , decreased expression of CD 40 ligand , and different immunoglobulin G subclass production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand inhibits endothelial cell migration by increasing production of endothelial reactive oxygen species . ^^^ Activated T lymphocytes and platelets , which express high amounts of CD 40 ligand ( CD40L ) on their surface , contribute significantly to plaque instability with ensuing thrombus formation , leading to acute coronary syndromes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated circulating soluble CD 40 ligand in patients with mixed connective tissue disease . ^^^ Abnormalities of CD 40 ligand have been demonstrated in various kinds of diseases . ^^^ Our objective was to determine serum soluble CD 40 ligand ( sCD40L ) levels in patients with mixed connective tissue disease ( MCTD ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibitory effects of glycoprotein IIb / IIIa antagonists and aspirin on the release of soluble CD 40 ligand during platelet stimulation . ^^^ However , platelet activation and subsequent aggregation are now known to cause the release of a soluble form of CD 40 ligand ( sCD40L ) , a prothrombotic and proinflammatory protein with GP IIb / IIIa binding activity and an established role in atherosclerotic lesion progression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In a previous study we showed that immunization with dendritic cells ( DC ) pulsed with idiotype ( Id ) fused with CD 40 ligand ( CD40L ) could break the tolerance to Id which is expressed on B lymphoma cells and restored the responsiveness of T ( h ) cells , and , subsequently , induced IgG antibody response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The aim of this study was to evaluate serum levels of both soluble CD 30 ( sCD 30 ) and soluble CD 40 ligand ( sCD40L ) in patients with malignant bone tumours and to determine their ability to serve as serum markers . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cooperation of TNF family members CD 40 ligand , receptor activator of NF kappa B ligand , and TNF alpha in the activation of dendritic cells and the expansion of viral specific CD8+ T cell memory responses in HIV 1 infected and HIV 1 uninfected individuals . ^^^ We systematically evaluated the ability of three TNF superfamily molecules , CD 40 ligand ( CD40L ) , receptor activator of NF kappaB ligand ( RANKL ) , and TNF alpha , to expand ex vivo EBV specific CTL responses in healthy human individuals and ex vivo HIV 1 specific CTL responses in HIV 1 infected individuals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Monocytes / macrophages activated by Th 1 stimulation such as interferon gamma ( IFN gamma ) and CD 40 ligand ( CD40L ) infiltrate the kidney and play a critical role in the progression of lupus nephritis ( LN ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand trimer enhances the response of CD8+ T cells to Mycobacterium tuberculosis . ^^^ We investigated the effect of recombinant CD 40 ligand trimer ( CD40LT ) on the functional capacity of peripheral blood CD 8 ( + ) T cells from healthy tuberculin reactors that were cultured with Mycobacterium tuberculosis infected autologous monocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cloning , sequencing and expression of porcine CD 40 ligand in Escherichia coli and human and porcine cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The experimental groups were recipients of limb allografts from C57Bl / 6 mice ( H 2K ( b ) ) and received either no treatment ( n=5 ) or treatment with MR 1 ( hamster antimouse CD 40 ligand monoclonal antibody ) 500 microg intraperitoneally ( IP ) on days 0 , 2 , 4 , 6 , 14 , 28 , and 60 ( n=5 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of the action of certain specific proinflammatory cytokines , such as CD 40 ligand , interferes with atherogenesis in mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Although IL 12 production by this dendritic cell like phenotype required additional stimulation with CD 40 ligand , this cell type spontaneously up regulated IL 15 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast to all other chemokines investigated to date , CCL 18 was selectively down regulated during the maturation process induced by LPS , TNF , CD 40 ligand , Staphylococcus aureus Cowan 1 , Candida albicans , and influenza virus . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , the level of PDMP was significantly correlated with that of RANTES or soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Prior treatment with CD 40 ligand normalized subsequent responses of xid B cells to BCR cross linking , so that typical outcomes of BCR signaling such as NF kappa B activation and cell cycle progression occurred in a Btk independent fashion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand assessing risk instead of damage . ^^^ Soluble CD 40 ligand in acute coronary syndromes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand in acute coronary syndromes . ^^^ BACKGROUND : CD 40 ligand is expressed on platelets and released from them on activation . ^^^ We investigated the predictive value of soluble CD 40 ligand as a marker for clinical outcome and the therapeutic effect of glycoprotein IIb / IIIa receptor inhibition in patients with acute coronary syndromes . ^^^ METHODS : Serum levels of soluble CD 40 ligand were measured in 1088 patients with acute coronary syndromes who had previously been enrolled in a randomized trial comparing abciximab with placebo before coronary angioplasty and in 626 patients with acute chest pain . ^^^ RESULTS : The levels of soluble CD 40 ligand were elevated ( above 5 . 0 microg per liter ) in 221 patients with acute coronary syndromes ( 40 . 6 percent ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Hyper IgM syndrome ( HIM ) is a rare disorder resulting from mutation in the CD 40 ligand ( CD40L ) gene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cultured ocular ECs and stromal cells were stimulated with various inflammatory mediators ( endotoxin ; TNFalpha ; interferon gamma ; interleukin ( IL ) 1alpha , 4 , 10 , 13 , 17 , and 18 ; and / or CD 40 ligand , or combinations thereof ) , with FKN mRNA being subsequently evaluated by cDNA array and / or RT PCR and FKN protein by enzyme linked immunoculture assay ( ELICA ) and / or by Western blot analysis . ^^^ All ocular microvascular ECs and stromal cultures expressed low FKN mRNA and / or protein levels , which were variably upregulated by endotoxin , TNFalpha , interferon gamma , IL 1alpha , and / or CD 40 ligand , but not by IL 18 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : CBA mice were given donor splenocytes ( 1x107 ) or Kb peptide ( 100 microg ) by ITD with or without antibody specific for mouse CD 40 ligand ( MR 1 , 200 microg ) 7 days before transplantation of a C57BL / 10 heart . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using oligonucleotide microarray technology we performed a genome wide analysis to investigate the transcriptional program in immature human monocyte derived DC induced to mature with either CD 40 ligand ( CD40L ) , lipopolysaccharide ( LPS ) or a cocktail of inflammatory cytokines and prostaglandin ( PG ) E ( 2 ) ( CyC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The co expression of bfl 1 was associated with the upregulation of CD 95 and CD 40 ligand ( CD40L ) in vitro and with pronounced intratumoral T lymphocyte infiltration in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Shear induced von Willebrand factor mediated platelet surface translocation of the CD 40 ligand . ^^^ We focused on the surface translocation of CD 40 ligand ( CD40L ) molecules when the platelets are exposed to a high shear stress . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Efficacy of CD 40 ligand gene therapy in malignant mesothelioma . ^^^ Gene delivery of CD 40 Ligand ( CD40L ) has shown promise in murine models of melanoma and adenocarcinoma ; however , its potential for thoracic malignancies such as malignant mesothelioma remains unclear . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we describe how the NF kappa B activating scaffold proteins involved in the signaling pathways triggered by the pro inflammatory cytokines TNF alpha , IL 1 beta and by the CD 40 ligand play such roles . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TRANCE and CD 40 ligand matured dendritic cells reveal MHC class 1 restricted T cells specific for autologous tumor in late stage ovarian cancer patients . ^^^ RESULTS : TRANCE and CD 40 ligand were effective at maturing DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Twenty eight patients with primary immunodeficiencies were studied including : CD 40 ligand deficiency ( 13 ) ; undefined combined immunodeficiency ( 10 ) ; major histocompatibility complex 2 deficiency ( 2 ) ; and other defects ( 3 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NKT cells express high levels of IL 18 receptor ( R ) alpha chain and produce significant amounts of IL 4 , IL 9 , and IL 13 , and induce CD 40 ligand expression in response to IL 2 and IL 18 stimulation in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand blocks apoptosis induced by tumor necrosis factor alpha , glucocorticoids , and etoposide in osteoblasts and the osteocyte like cell line murine long bone osteocyte Y 4 . ^^^ It was observed that the MLO Y 4 osteocyte like cells express CD 40 antigen and MHC class 1 antigen , but they are negative for a series of other dendritic cells markers ( DEC 205 , CD11b , CD11c , CD 86 , and MHC class 2 ) and immune cell markers [ CD 45 , CD 3 , CD 4 , B 220 , Gr 1 , and CD 40 ligand ( CD40L ) ] . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
For example , GM CSF has been shown to be synergistic with IL 12 or CD 40 ligand for induction of CTL and for antiviral protection , and the triple combination of GM CSF , IL 12 , and TNF alpha appears to induce the most effective protection in some mouse models . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression constructs were made expressing the hyperfusogenic version of the Gibbon Ape Leukemia Virus envelope glycoprotein ( GALV ) linked to a blocking ligand ( the C terminal extracellular domain of CD 40 ligand ) via either an MMP cleavable linker ( GALV M 40 ) , a factor Xa protease cleavable linker ( GALV X 40 ) , or a noncleavable linker ( GALV N 40 ) . ^^^ Our data indicate that GALV induced cytotoxicity in glioma cell lines can be blocked by display of the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DC exposed to GA have an impaired capacity to secrete the major Th 1 polarizing factor IL 12p70 in response to LPS and CD 40 ligand triggering . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Maturation of DCs in response to poly ( IC ) but not to CD 40 ligand did overcome the inhibitory effect of RSV . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Since CD 40 ligand and IL 4 are produced by activated T helper cells , these results indicate that TARC and MDC produced by B cells play important roles in the production of antigen specific IgE by the T B cell interaction and in the pathogenesis of allergic disease . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thirteen Japanese patients with hyper IgM syndrome but normal CD 40 ligand were characterized . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : MPhi from peripheral blood ( PBMPhi ) or cord blood ( CBMPhi ) were stimulated with interferon gamma ( IFN gamma ) , cyclic adenosine monophosphate ( cAMP ) , CD 40 ligand ( CD40L ) , or alphaCD 3 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Bystander transfer of functional human CD 40 ligand from gene modified fibroblasts to B chronic lymphocytic leukemia cells . ^^^ CD 40 ligand ( CD40L ) is a good candidate molecule for the immunotherapy of B cell malignancies including B chronic lymphocytic leukemia ( B CLL ) , because it may increase the capacity of the malignant cells to present tumor antigens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , the T helper ( Th ) cells , and also B cells of lupus , have a regulatory defect in the expression of CD 40 ligand ( CD40L ) . ^^^
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The two major subsets of invariant NKT cells , CD4+ and double negative ( CD 4 CD8 ) , express comparable levels of CD 40 ligand and cytokines , but differ in helper functions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this paper , we discuss this function , defined as cross presentation , and how it is directed , particularly in inducing T cell tolerance , and how it requires special activating signals ( such as CD 40 ligand ) to transform into a mechanism that provides either protective immunity or autoimmunity . . ^^^
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The recent demonstration that activated platelets express CD 40 ligand ( L ) provides a mechanism of interaction with CD 40 positive endothelial cells , inducing them to produce proinflammatory mediators . ^^^
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NF kappaB is involved in regulation of CD 40 ligand expression on Mycobacterium bovis bacillus Calmette Gurin activated human T cells . ^^^
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Here , we compared the functional capacity of FL expanded CD1c+ PBDCs with autologous MoDCs in response to 3 different classes of stimuli : ( 1 ) proinflammatory mediators , ( 2 ) soluble CD 40 ligand trimer ( CD40L ) , and ( 3 ) intact bacteria ( Escherichia coli ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated plasma levels of the atherogenic mediator soluble CD 40 ligand in diabetic patients : a novel target of thiazolidinediones . ^^^ BACKGROUND : Considerable evidence implicates the proinflammatory cytokine CD 40 ligand ( CD40L ) in atherosclerosis and accumulating data link type 1 and 2 diabetes , conditions associated with accelerated atherosclerosis , to inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expression of CD 86 by CML DCs was enhanced when they were cultured with IFN alpha / IL 4 / GM CSF , or when IFN alpha / GM CSF treated cells were induced to mature by CD 40 ligand . ^^^
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The induced expression of the CD 40 Act1 axis by inflammatory cytokines in epithelial cells probably plays an important role in priming these cells for their response to CD 40 ligand during airway inflammation . . ^^^
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Retention and defective assembly of the B cell receptor in the endoplasmic reticulum of chronic lymphocytic leukaemia B cells can not be reverted upon CD 40 ligand stimulation . ^^^
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T cell dependent antitumor effects produced by CD 40 ligand expressed on mouse lung carcinoma cells are linked with the maturation of dendritic cells and secretion of a variety of cytokines . ^^^
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Monocytes produced NE constitutively , with little regulation by cytokines IL 1beta , TNF alpha , or IFN gamma but released it when stimulated by CD 40 ligand , a cytokine found in atheroma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Study of CD 40 ligand expression on mitogen activated peripheral blood mononuclear cells revealed total absence of this marker on T lymphocytes . ^^^ Molecular analysis detected , in the patient and his mother , a nonsense mutation in exon 1 of the transmembrane segment of the CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 4 , CD8alpha , CD 40 , CD 86 , CD 28 , CD 31 and vascular endothelial growth factor was not different between the two tumor groups but expression of CD 40 ligand , CD 80 , NK1 . 1 and CXCR 3 was relatively lower in A11 / Mig tumors . ^^^
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The expression of MHC class 2 , CD 86 , and CD 83 Ags on DCs and CD 40 ligand ( L ) associated IL 12 p 70 production from DCs were up regulated by the membrane Ags . ^^^
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CD 40 ligand ( CD 154 ) improves the durability of respiratory syncytial virus DNA vaccination in BALB / c mice . ^^^ In this study , we examine CD 40 ligand ( CD40L ) as an immune modulator to enhance the durability of DNA vaccines encoding RSV F and / or G glycoproteins in BALB / c mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is a critical effector of Epstein Barr virus in host cell survival and transformation . ^^^ Here we found that EBV equally infected B cells from patients with 10 linked hyper IgM syndrome and those from healthy donors ; however , it hardly transformed 10 linked hyper IgM syndrome B cells , because of the dysfunctional gene of CD 40 ligand ( CD40L ) of the patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand levels indicate lipid accumulation in carotid atheroma : an in vivo study with high resolution MRI . ^^^ We tested the hypothesis that plasma levels of soluble CD 40 ligand are elevated in patients with evidence of a lipid pool on high resolution magnetic resonance imaging ( MRI ) of carotid stenoses . ^^^ Plasma levels of soluble CD 40 ligand were determined by ELISA . ^^^ Baseline levels of soluble CD 40 ligand were higher among patients with evidence of intraplaque lipid ( n=14 ) than among those without it ( n=32 ; median , 2 . 54 ng / mL ; interquartile range [ IQR ] , 1 . 85 to 3 . 52 vs median , 1 . 58 ng / mL ; IQR , 1 . 21 to 2 . 39 ; P=0 . 02 ) . ^^^ In contrast , soluble CD 40 ligand levels were not correlated with percent diameter stenosis ( r= 0 . 19 ; P=0 . 21 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immature DC showed reduced ability to produce IL 12p70 and tumor necrosis factor ( TNF ) alpha upon lipopolysaccharide or CD 40 ligand ( CD40L ) stimulation in the presence of PDE 4 inhibitors , whereas cytokine production upon CD40L stimulation of fully mature DC in the presence of PDE 4 inhibitors was not affected . ^^^
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Deficiency in the membrane glycoprotein CD 40 ligand ( expressed on activated T cells ) compromises T cell interactions with antigen presenting cells . ^^^
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From gene to disease ; CD 40 ligand deficiency as the cause of 10 linked hyper IgM syndrome ] . 10 linked hyper IgM syndrome ( XHIM ) is a rare congenital immunodeficiency disorder , characterised by a defect in both humoral and cellular immune responses . ^^^ In XHIM , the membrane glycoprotein CD 40 ligand ( expressed on activated T cells ) is deficient , which compromises T cell interactions with antigen presenting cells . ^^^ The gene encoding the CD 40 ligand is located on the long arm of the 10 chromosome . . ^^^
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We demonstrate that the alpha chain of human C4b binding protein ( C4BP ) binds directly to CD 40 on human B cells at a site that differs from that used by CD 40 ligand . ^^^
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Efficient VLP cross presentation by Langerhans cells with costimulation can be achieved by addition of CD 40 ligand . ^^^
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The molecular defects that have so far been associated with this syndrome which affect the CD 40 ligand in HIGM type 1 ( HIGM 1 ) , CD 40 in HIGM 3 , and activation induced cytidine deaminase ( AID ) in HIGM 2 do not account for all cases . ^^^
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Induction of an Ig class switch in nude mice was related to the expression of IFN gamma and CD 40 ligand in the draining lymph nodes . ^^^
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RESULTS : IKK 2 was not required for lipopolysaccharide ( LPS ) induced NF kappaB activation or TNFalpha , IL 6 , or IL 8 production in macrophages , but was essential for this process in response to CD 40 ligand , TNFalpha , and IL 1 . ^^^
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Why do B cells produce CD 40 ligand . ^^^
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Both interleukin 4 and CD 40 ligand are important for optimal dendritic cell differentiation and maturation . ^^^ Granulocyte monocyte colony stimulating factor , interleukin 4 , and CD 40 ligand in combination are capable of yielding dendritic cells from at least some cases of acute lymphocytic leukemia . ^^^
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The activity of PKC and [ Ca2+ ] 1 induced by CD 40 ligand ( CD40L ) in PBMC were measured by its ability to transfer phosphate from [ gamma 32P ] ATP to lysine rich histone and flow cytometric analysis loading with the Ca2+ dye Fluo 3 / Am , respectively . ^^^
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The cells of chronic lymphocytic leukemia were treated in vitro with CD 40 ligand or IL 4 to explore their effects on survival and sensitivity to apoptosis induced by Fluda . ^^^
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Three novel mutations reflect the variety of defects causing phenotypically diverse 10 linked hyper IgM syndrome . 10 linked hyper IgM syndrome ( HIGM 1 ) ( MIM musical sharp 308230 ) , is a severe primary immunodeficiency caused by mutations in the gene coding for CD 40 ligand ( CD40L or CD 154 ) , a member of the tumour necrosis factor ( TNF ) superfamily . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : Keratinocytes were grown on fibroblasts transfected with the CD 40 ligand ( CD40L ) to investigate the formation of epidermal sheets in culture under the influence of the CD40L . ^^^
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The results showed that the capacity of circulating DCs was significantly impaired in subjects with CD4+ T cell counts of < 200 / microL , correlated with the potential of CD 40 ligand expression on CD4+ T cells ( R = 0 . 84 ; P = 0 . 002 ) , and improved with successful antiretroviral therapy . ^^^ Our findings suggest that although the potential of myeloid DC precursors to achieve full maturation is preserved in subjects with late stage HIV 1 infection , in vivo maturation of myeloid DCs was impaired in these subjects , which may be due to decreased potential of CD 40 ligand expression on CD4+ T cells . ^^^
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CD 154 ( CD 40 ligand ) deficient mice exhibit prolonged bleeding time and decreased shear induced platelet aggregates . ^^^
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The effects of resistin on expression of vascular cell adhesion molecule ( VCAM 1 ) and monocyte chemoattractant chemokine ( MCP 1 ) were studied in addition to CD 40 receptor , CD 40 ligand induced MCP 1 expression , and tumor necrosis factor receptor associated factor 3 ( TRAF 3 ) , an inhibitor of CD 40 signaling . ^^^ Resistin did not alter CD 40 receptor expression ; however , increased CD 40 ligand induced MCP 1 production . ^^^ Furthermore , resistin upregulates adhesion molecules and chemokines and downregulates TRAF 3 , an inhibitor of CD 40 ligand signaling . ^^^
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When challenged , CD 4 ( + ) T cells from FoxP 3 Tg mice display reduced up regulation of CD 40 ligand and fewer IFN gamma producing cells . ^^^
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CD 40 ligand is a cell surface molecule on CD 4 ( + ) T cells that interacts with its receptor , CD 40 , on antigen presenting cells to mediate humoral and cellular immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : Elevated plasma concentrations of soluble CD 40 ligand ( sCD40L ) indicate increased risk for future cardiovascular events in apparently healthy women . ^^^
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NK cell induced DC 1 show a strongly elevated ability to produce IL 12p70 after subsequent CD 40 ligand stimulation . ^^^
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C5a transformed monocytes represented an inflammatory type of DC , as they constitutively secreted high amounts of TNF alpha , but also retained the capacity to release the Th 1 cytokine IL 12 p 70 upon stimulation with CD 40 ligand . ^^^
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PURPOSE : To examine the effect of modulating the lymphocyte costimulation pathways through CD 28 and CD 154 ( CD 40 ligand ) in a model of corneal allograft rejection , with particular interest in changes in the observed features of rejection . ^^^
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CD 40 ligand is essential for generation of specific cytotoxic T cell responses in RNA pulsed dendritic cell immunotherapy . ^^^ This study evaluated the effects of exogenous soluble CD 40 ligand ( sCD40L ) on RNA transfected DC preparations and their subsequent ability to generate antimelanoma cytolytic T cells . ^^^
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Tumor cytolytic responses were enhanced by the addition of CD 40 ligand ( CD40L ) , and partially blocked by anti MHC class 1 antibody . ^^^
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Mutations of CD 40 ligand in two patients with hyper IgM syndrome . ^^^
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BMM phi differentiated into dendritic cells when treated with granulocyte macrophage colony stimulating factor together with CD 40 ligand , TNF alpha , or LPS , and SB 203580 failed to inhibit this differentiation . ^^^
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Role of CD 40 ligand dysregulation in HIV associated dysfunction of antigen presenting cells . ^^^
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CD 40 ligand synergistically increased CpG B induced IFN alpha independent of the IFNR but did not affect CpG B induced IFN beta . ^^^
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Characterizing the cells in more detail , we could demonstrate for the first time that normal human LCs express CXCR 4 , CD 40 ligand ( CD40L ) , and Fas and Fas ligand ( FasL ) . ^^^
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Activated platelets are the source of elevated levels of soluble CD 40 ligand in the circulation of inflammatory bowel disease patients . ^^^
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Interaction of T helper cells expressing CD 40 ligand ( CD40L ) with its cognate CD 40 receptor on DCs leads to a mature DC phenotype , characterized by increased capacity of antigen presentation to cytotoxic T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Serial measurement of surface expressions of CD 63 , P selectin and CD 40 ligand on platelets in atherosclerotic ischemic stroke . ^^^ A possible role of CD 40 ligand on platelets in atherosclerotic ischemic stroke . ^^^ The CD 40 ligand ( CD40L ) on platelets may be a critical factor to develop the acute vascular events from atheroma . ^^^
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In this study , we found that addition of the NO donor S nitrosoglutathione ( GSNO ) to monocyte derived DCs matured by lipopolysaccharide ( LPS ) or soluble CD 40 ligand led to a decreased capacity to activate naive allogeneic T cells but a more prominent Th 1 polarization , with increased interferon gamma ( IFN gamma ) secretion and reduced interleukin 5 ( IL 5 ) release . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Combination of monocyte derived dendritic cells and activated T cells which express CD 40 ligand : a new approach to cancer immunotherapy . ^^^ To develop the basis for a new DC based cancer vaccine , we investigated cell to cell interactions between human monocyte derived DCs and autologous T cells that are activated to express the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Long term allograft survival in the absence of continuous immunosuppression can be induced following a short treatment of nondepleting antibodies , such as those that target CD 4 or CD 154 ( CD 40 ligand ) . ^^^
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After maturation with CD 40 ligand , IFN DCs up regulated costimulatory , class 1 and 2 MHC molecules and expressed mature DC markers such as CD 83 and DC lysosome associated membrane protein . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Bone marrow derived DC stimulated with Escherichia coli O 26 : B 6 LPS produced IL 12p70 for a limited period of time , after which production became refractory to further stimulation with CD 40 ligand , a phenomenon previously called `` exhaustion . ' ' The level of IL 12 production of DC did not correlate with Th 1 development , as exhausted OVA pulsed DC were still capable of shifting the cytokine pattern of responding OVA specific Th cells toward Th 1 in vitro and in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mechanism of allorecognition and skin graft rejection in CD 28 and CD 40 ligand double deficient mice . ^^^ To uncover the mechanisms underlying these costimulation blockade resistant allograft rejections , we studied the process of allogenic skin graft rejection in CD 28 and CD 40 ligand ( L ) double deficient ( double knockout [ dKO ] ) mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expression of two genes related to B cell responses , B lymphocyte chemoattractant , and CD 40 ligand , showed a similar time course to that of the immunoglobulin kappa chain gene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interaction of CD 40 on dendritic cells ( DC ) with CD 40 ligand induces interleukin 12 ( IL 12 ) production by these DC during the antigen presentation . ^^^
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Neurotrophic properties of human MDM conditioned media are significantly enhanced by human peripheral nerve and to a more limited extent by CD 40 ligand pre stimulation . ^^^
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BACKGROUND : 10 linked hyper IgM ( XHIM ) is a primary immunodeficiency caused by mutations in the tumour necrosis factor superfamily 5 gene , TNFSF 5 , also known as the CD 40 ligand ( CD40L ) gene . ^^^
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Activation of cross priming by IFN alpha / beta was independent of CD4+ T cell help or interaction of CD 40 and CD 40 ligand , and involved direct stimulation of dendritic cells . ^^^
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CD 40 ligand is selectively expressed on CD4+ T cells and platelets : implications for CD 40 CD40L signalling in atherosclerosis . ^^^ Endothelial cells ( ECs ) , smooth muscle cells , and macrophages , key elements in atherosclerosis , all have the potential to express the CD 40 receptor and are thus susceptible to potent pro inflammatory signals by CD 40 ligand ( CD40L ) bearing cells . ^^^
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Soluble CD 40 ligand induces beta 3 integrin tyrosine phosphorylation and triggers platelet activation by outside in signaling . ^^^ We earlier reported that the soluble form of the CD 40 ligand ( sCD40L ) , is involved in thrombosis by stabilizing platelet thrombi . ^^^
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Further support for this concept comes from animal models of autoimmunity and transplantation , where treatments based on costimulation blockade , in particular CD 40 ligand ( CD40L ) specific antibodies , have been highly effective . ^^^
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Treatment of CD 40 ligand deficiency by hematopoietic stem cell transplantation : a survey of the European experience , 1993 2002 . ^^^ CD 40 ligand ( CD40L ) deficiency causes recurrent sinopulmonary infection , Pneumocystis carinii pneumonia , and Cryptosporidium parvum infection . ^^^
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MPO levels did not correlate with troponin T , soluble CD 40 ligand , or C reactive protein levels or with ST segment changes . ^^^ In a multivariate model that included other biochemical markers , troponin T ( HR 1 . 99 ; P=0 . 023 ) , C reactive protein ( 1 . 25 ; P=0 . 044 ) , vascular endothelial growth factor ( HR 1 . 87 ; P=0 . 041 ) , soluble CD 40 ligand ( HR 2 . 78 ; P < 0 . 001 ) , and MPO ( HR 2 . 11 ; P=0 . 008 ) were all independent predictors of the patient ' s 6 month outcome . ^^^
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The tropism of the virus was modified by the incorporation into its capsid of a protein chimera comprising structural domains of three different proteins : the Ad serotype 5 fiber , phage T 4 fibritin , and the human CD 40 ligand ( CD40L ) . ^^^
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In the present study , we examined the effects of prostaglandin E 1 ( PGE 1 ) on the expression of intercellular adhesion molecule ( ICAM ) 1 , B7 . 1 , B7 . 2 , CD 40 , and CD 40 ligand ( CD40L ) on peripheral blood mononuclear cells ( PBMC ) using fluorescence activated cell sorting analysis as well as its effects on cytokine production using enzyme linked immunosorbent assay . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation by CD 40 ligand ( L ) improves B cell malignancy immunogenicity , and also induces proliferative signals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of T cell tolerance in a patient with idiopathic thrombocytopenic purpura by single injection of humanized monoclonal antibody to CD 40 ligand . ^^^ Normal B cells can be induced to express immune costimulatory molecules by activated T cells , and activated CD 4 T cells can express CD 40 ligand , a molecule that can engage CD 40 on the B cell surface . ^^^ We report a patient with chronic idiopathic thrombocyopenic purpura ( ITP ) who was effectively treated with a single injection of humanized monoclonal antibody to CD 40 ligand ( E 6040 ) . ^^^ A single injection of humanized monoclonal antibody to CD 40 ligand may induce T cell tolerance in patients with ITP . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of CD 40 CD 40 ligand interaction on diacylglycerol protein kinase C and inositol trisphosphate Ca ( 2+ ) signal transduction pathway in human umbilical vein endothelial cells . ^^^ METHODS : Endothelial cells were isolated from human umbilical vein and incubated with indicated concentrations of CD 40 ligand ( CD40L ) for various periods . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) deficient C57BL / 6 mice failed to control intracellular Leishmania donovani visceral infection , indicating that acquired resistance involves CD 40 CD40L signaling and costimulation . ^^^
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Concentrations of C reactive protein ( CRP ) , interleukin 6 ( IL 6 ) , and interleukin 1 receptor antagonist ( IL 1Ra ) and soluble CD 40 ligand ( sCD40L ) were measured using ELISA . ^^^
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C reactive protein ( CRP , microg / ml ) , interleukin ( IL ) 6 ( pg / ml ) , IL 1 receptor antagonist ( pg / ml ) , and soluble CD 40 ligand ( ng / ml ) were assayed in each sample by enzyme linked immunosorbent assay . ^^^ Preprocedural concentrations of IL 6 , IL 1 receptor antagonist , and soluble CD 40 ligand tended to be greater in patients with diabetes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The majority of cytoplasmic TRAF 2 GFP and , to a lesser extent , TRAF 3 GFP , but not TRAF 1 GFP or TRAF 4 GFP , translocated into CD 40 signaling complexes within a few minutes after CD 40 triggering with the CD 40 ligand . ^^^
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Genetically , at least 4 distinct molecular defects have been identified that result in defective CSR and present as HIGM syndromes : defects of the CD 40 ligand gene ( CD40L ; HIGM 1 , 10 linked ) , the activation induced cytidine deaminase gene ( AID ; HIGM 2 , autosomal recessive ) , the CD 40 gene ( HIGM 3 , autosomal recessive ) , and the nuclear factor kappaB ( NF kappaB ) essential modulator gene ( NEMO , or IKK gamma , 10 linked ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand dependent maturation of human monocyte derived dendritic cells by activated platelets . ^^^ This effect was inhibited by anti CD 40 ligand antibody , but not by aspirin pretreatment of platelets . ^^^ Supernatants of platelet dendritic cell co cultures induced augmented monocyte migration when platelets were activated by thrombin , again reversible by blocking CD 40 ligand . ^^^ These data show that activated platelets trigger dendritic cell maturation independent of cyclooxygenase derived arachidonic acid metabolites by mechanisms involving CD 40 ligand , which is also involved in monocyte chemotactic mediator release from platelets and dendritic cells . ^^^ The results of this study suggest a role of CD 40 ligand from activated platelets in connecting innate and adaptive immunity . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this randomized double blind study , we compared the efficacy of simvastatin and fenofibrate on indexes of endothelial function ( flow mediated dilation ( FMD ) of the brachial artery ) and inflammatory markers ( plasma high sensitivity C reactive protein ( CRP ) , interleukin 1 beta ( IL 1 beta ) , soluble CD 40 , and soluble CD 40 ligand ( sCD40L ) levels ) , as surrogate indicators of future coronary heart disease ( CHD ) , in patients with combined hyperlipidemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Therefore , the ICOS is after CD 40 and CD 40 ligand deficiency the third costimulatory molecule for which a human disease has been described . ^^^
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Monocyte platelet aggregate formation and monocyte tissue factor expression were determined by whole blood flow cytometry , and F1 . 2 generation and soluble CD 40 ligand ( sCD40L ) were determined by immunoassay . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We report a case of a 6 month old boy who suffered from developmental delays , frequent respiratory tract infection , and unusual fungal and bacterial infection . 10 linked hyperimmunoglobulin M syndrome was ultimately diagnosed with decreasing immunoglobulin G , A , and E ( immunoglobulin G = 51 . 3 mg / dL , immunoglobulin A = 8 . 32 mg / dL , immunoglobulin E < 17 . 5 mg / dL ) , elevating immunoglobulin M ( immunoglobulin M = 140 mg / dL ) , and decreasing T cell expression of the CD 40 ligand over flow cytometry . ^^^
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Enhanced levels of CD 154 ( CD 40 ligand ) on platelets in patients with chronic heart failure . ^^^
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Class switching occurs through class switch DNA recombination ( CSR ) and usually requires engagement of CD 40 on B cells by CD 40 ligand on Ag activated CD 4 ( + ) T cells . ^^^
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This adenoviral vector encodes a fusion protein composed of an amino terminal tumor associated antigen fragment fused to the CD 40 ligand ( CD40L ) . ^^^
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In order to investigate associations with host immune reactions and hsp 60 antigen , CD 40 ligand ( CD40L ) expression and cytokine production were analysed following stimulation with hsp 60 . ^^^
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The 10 linked hyper IgM ( XHIGM ) syndrome is an uncommon primary immunodeficiency disease caused by mutations in the gene for CD 40 ligand and characterized by normal or elevated serum IgM , reduced levels of IgG and IgA , and defective T cell function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Wild type and Fas ligand deficient mice bearing Lewis lung carcinoma flank tumors were then treated with intraperitoneal cisplatin as well as an intratumoral injection of an adenovirus gene transfer vector encoding CD 40 ligand . ^^^ Cytotoxicity assays were performed using splenocytes from adenovirus gene transfer vector encoding CD 40 ligand treated animals as effectors and cisplatin treated Lewis lung carcinoma cells as targets . ^^^ In vivo , the combination of 1 dose of intraperitoneal cisplatin and intratumoral adenovirus gene transfer vector encoding CD 40 ligand inhibited tumor growth and prolonged survival compared with adenovirus gene transfer vector encoding CD 40 ligand alone , resulting in a higher cure rate . ^^^ Splenocytes from adenovirus gene transfer vector encoding CD 40 ligand treated wild type mice lysed cisplatin treated Lewis lung carcinoma cells more efficiently than untreated Lewis lung carcinoma cells , an effect lost in splenocytes from Fas ligand deficient mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In 3 4 , 5 dimethylthiozol 2 yl 2 , 5 diphenyl tetrazolium bromide ( MTT ) assay and Annexin V / propidium iodide staining , CD 40 stimulation using a soluble form of CD 40 ligand did not affect cell viability , but significantly inhibited Fas mediated or chemotherapy mediated apoptosis in three CD 40 positive gastric cancer cell lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition the interaction between the CLL cells and the immune system , which has been exploited in gene therapy with transfection of CLL cells by CD 40 ligand , is discussed . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation of T cells increases their ability to induce class 2 enhanced green fluorescent protein positive tubules in dendritic cells , in part through up regulation of CD 40 ligand . ^^^
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METHODS : Diagnoses were based on immunoglobulin results and the absence of CD 40 ligand ( CD40L ) expression on activated T cells . ^^^
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Functional significance of CD 40 and CD 40 ligand linking on human lung cells ] . ^^^ OBJECTIVE : To determine the possible functional significance of CD 40 and CD 40 ligand ( CD 154 ) linking on human lung carcinomas and to assess the potential of CD 40 as a therapeutic target . ^^^
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The sequence of CD 40 ligand and cytidine deaminase genes were normal . ^^^
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Here , we cultured circulating monocytes for five days with interleukin 4 and GM CSF followed by two day culture with or without CD 40 ligand and LPS to create a mature DC ( mDC ) and an immature DC ( iDC ) phenotype , respectively , characterized by differential expression of co stimulatory molecules ( CD 80 , CD 83 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction between CD 40 ligand ( CD 154 ) expressed on activated T cells and its receptor , CD 40 , has been shown to play a role in the onset and maintenance of autoimmune inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upregulation of CD 40 CD 40 ligand system in patients with diabetes mellitus . ^^^
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The capacity of NK cells to prime CD8+ T cells to lyse M . tuberculosis infected target cells required cell cell contact between NK cells and infected monocytes and depended on interactions between the CD 40 ligand on NK cells and CD 40 on infected monocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
TCR signal strength and IL 12 production from dendritic cells ( DCs ) are linked by CD 40 ligand ( CD40L ) expression on activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased coronary arterial release of interleukin 1 receptor antagonist and soluble CD 40 ligand indicative of inflammation associated with culprit coronary plaques . ^^^ The concentrations of C reactive protein ( CRP ) , interleukin ( IL ) 6 , IL 1 receptor antagonist , and soluble CD 40 ligand ( sCD40L ) were measured in blood drawn from 75 patients before percutaneous coronary intervention from the femoral artery and from a guide catheter after engagement of the culprit coronary artery . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We investigated cell adhesion molecule ( CAM ) up regulation and chemokine production by CD 40 positive human intestinal fibroblasts ( HIF ) and microvascular endothelial cells ( HIMEC ) induced by CD 40 ligand ( CD40L ) positive T cells and soluble CD40L and their effect on T cell adhesion and transmigration . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 12p70 dependent Th 1 induction by human B cells requires combined activation with CD 40 ligand and CpG DNA . ^^^ Similar to CpG ODN , CD 40 ligand ( CD40L ) alone induced IL 6 , TNF alpha , and IL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
P selectin , tissue factor and CD 40 ligand expression on platelet leucocyte conjugates in the presence of a GPIIb / IIIa antagonist . ^^^ This study was to investigate the appearance of P selectin , tissue factor ( TF ) and CD 40 ligand ( CD40L ) on platelet leucocyte conjugates in the absence and presence of a GPIIb / IIIa antagonist , MK 852 , and the effect of adding EDTA to pre formed conjugates . ^^^ EDTA displaced the majority of platelets from the conjugates and also the P selectin , TF and CD40L , whereas it did not displaced P selectin or CD 40 ligand from the platelets themselves . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In studies to evaluate whether the interaction between CD8+ cells and dendritic cells ( DCs ) could increase CNAR , CD8+ cells from individuals who showed a decrease in this antiviral activity were cocultured with monocyte derived dendritic cells matured with CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , TRANCE blockade induced strong up regulation of CD 40 ligand ( CD40L ) mRNA in allografts . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 154 ( CD 40 ligand ) by human lung fibroblasts : differential regulation by IFN gamma and IL 13 , and implications for fibrosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We examined the effects of endogenous catecholamines and beta 2 AR agonists on the expression of intercellular adhesion molecule ( ICAM ) 1 , B7 . 1 , B7 . 2 , CD 40 , and CD 40 ligand ( CD40L ) in human mixed lymphocyte reaction ( MLR ) and in an in vitro model of acute rejection in the presence or absence of IL 18 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
VEGF is expressed in a range of cells in response to soluble mediators ( such as cytokines and growth factors ) , cell bound stimuli ( such as CD 40 ligand ) , and environmental factors ( such as hypoxia ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
RESULTS : CD4+ T cells from MGS significantly expressed mRNA of interferon ( IFN ) gamma , interleukin ( IL ) 2 , granulocyte macrophage colony stimulating factor ( GM CSF ) , transforming growth factor ( TGF ) beta , tumor necrosis factor ( TNF ) alpha , IL 4 , CD 40 ligand ( CD40L ) , Fas ligand ( FasL ) and IL 2 receptor ( IL 2R ) during dry period , and mRNA of IFN gamma , IL 2 and TGF beta during lactation period . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : 10 linked hyper immunoglobulin M ( 10 HIM ) syndrome is a rare genetic immunodeficiency syndrome caused by mutations in the gene encoding CD 40 ligand ( CD40L , CD 154 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand plasma levels in lung cancer . ^^^ PURPOSE : Tumor induced platelet activation may cause the release of various cytokines , including CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The recent report that platelets express CD 40 led us to hypothesize that CD 40 ligand ( CD40L ) positive T cells could bind to platelets , cause their activation , and trigger granular RANTES release , creating a T cell recruitment feedback loop . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A short treatment with nondepleting antibodies , such as those targeting CD 4 or CD 154 ( CD 40 ligand ) , allows long term graft survival without the need for continuous immunosuppression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To this end , highly purified B cells from 10 HIV 1 untreated patients were cultured with or without mouse fibroblastic cells expressing the CD 40 ligand in the presence of IL 2 and IL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thus , upon activation , platelets release a series of cytokines and growth factors and express CD 40 ligand , which interacts with the CD 40 receptor on other major cell types involved in atherosclerosis / atherothrombosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DCs matured and further treated by soluble CD 40 ligand ( sCD40L ) had a 10 and 2 fold increase in MART antigen presentation compared to untreated ( immature ) DCs and DCs treated only with a first maturation signal , respectively ( Elispot P = 0 . 02 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It is known that parasites produce some types of molecule that mimic host molecules such as CD 40 ligand , TGF beta and macrophage migration inhibitory factor . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand , LPS or a combination of TNF alpha and prostaglandin E 2 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The help signal is one of the most potent stimuli for DC maturation and is provided by the interaction of CD 40 expressed on DCs with CD 40 ligand on CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 4 ( + ) T cells from children also had reduced CMV specific IL 2 and CD 154 ( CD 40 ligand ) expression , suggesting an early blockade in the differentiation of viral specific CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
By showing that nonmalignant B cells up regulate BAFF and APRIL upon stimulation by T cell CD 40 ligand , our findings indicate that NHL B cells deregulate an otherwise physiological autocrine survival pathway to evade apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
On day 1 , PBMC from NR patients had increased expression of IFN gamma ( p=0 . 006 ) , IL 10 ( p=0 . 016 ) , and CD 40 ligand ( p=0 . 02 ) compared with R . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Our main findings when comparing heart transplant recipients ( n = 52 ) and age and sex matched healthy controls ( n = 38 ) were : ( 1 ) platelets from heart transplant recipients showed enhanced expression of both P selectin and CD 63 as assessed by flow cytometry ; ( 2 ) platelets from these patients also contained significantly increased levels of soluble CD 40 ligand and tended to release higher levels of this cytokine upon SFLLRN stimulation as assessed by enzyme immunoassay ; ( 3 ) heart transplant recipients had increased levels of soluble P selectin in platelet free plasma ; and ( 4 ) the enhanced platelet activation after heart transplantation was most pronounced in those with concomitant hypertension . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Platelet expression of the inflammatory markers CD 40 ligand ( L ) and CD 62 P selectin ( P ) and serum levels of interleukin 6 and CD40L were compared in patients pretreated ( > 24 hours before PCI ) or not pretreated with clopidogrel . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand dysregulation in HIV infection : HIV glycoprotein 120 inhibits signaling cascades upstream of CD 40 ligand transcription . ^^^ IL 12 production and up regulation of CD 40 ligand ( CD40L ) expression are impaired in the PBMC of HIV infected donors , and exogenous CD40L rescues IL 12 production by such cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Enhanced P selectin expression and increased soluble CD 40 Ligand in patients with Type 1 diabetes mellitus and microangiopathy : evidence for platelet hyperactivity and chronic inflammation . ^^^ Von Willebrand factor antigen in plasma , serum soluble E selectin , CD 40 ligand ( sCD40L ) and C reactive protein ( CRP ) served as markers for endothelial function and inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , in the treated animals CD 40 ligand was expressed on day 3 , the expression was enhanced on day 7 , and was more pronounced on day 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human B cells isolated from peripheral blood were activated and induced to proliferate by either Epstein Barr virus ( EBV ) or the T cell derived mitogens CD 40 ligand ( CD40L ) plus interleukin ( IL ) 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Monocyte derived human DCs from normal donors were exposed to purified B 11 hCGbeta , activated with CD 40 ligand , mixed with autologous lymphocytes , and tested for their ability to promote hCGbeta specific proliferative and cytotoxic T lymphocyte responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 , expressed by lamina propria B lymphocytes in gut mucosa , interacts with CD 40 ligand on T cell . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NO synthase inhibition increased platelet activation as determined by enhanced platelet binding of fibrinogen and surface expression of P selectin , glycoprotein 53 , and CD 40 ligand , demonstrating tonic inhibition of platelet activation by NO production in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The modulation of CD 40 ligand signaling by transmembrane CD 28 splice variant in human T cells . ^^^ The role of CD 40 ligand ( CD40L ) / CD40 signaling in T cell dependent B cell differentiation and maturation has been amply documented . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that regulatory T cells render the DC inefficient as APCs despite prestimulation with CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this review , we highlight recent results on several of the more promising markers of inflammation for cardiovascular disease risk assessments , such as C reactive protein , myeloperoxidase , and soluble CD 40 ligand and nitrotyrosine , as well as other potential markers . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In a fully major histocompatibility complex ( MHC ) mismatched mouse model of islet transplantation , we demonstrate that while monotherapy with CTLA 4 Ig , CD 40 ligand monoclonal antibody ( CD40L mAb ) or rapamycin each improves islet allograft survival , graft rejection eventually develops . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Signalling through CD 40 and CD 40 ligand is a versatile pathway that is potently involved in all these processes . ^^^ Moreover , we discuss how CD 40 ligand at inflammatory sites stimulates fibroblasts and tissue monocyte / macrophage production of VEGF , leading to angiogenesis , which promotes and maintains the chronic inflammatory process . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 3 treated PDCs expressed OX40L , but produced almost no IFN alpha in response to T cell stimulation ( CD 40 ligand or T cell interaction ) , resulting in the preferential priming of Th 2 cells through OX40L dependent mechanisms . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Previously we found that CD 40 ligand ( CD 154 ) is functionally expressed on peritoneal macrophages during continuous ambulatory peritoneal dialysis peritonitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rapid disease development in scrapie infected mice deficient for CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CpG DNA and inflammatory stimuli , such as TNF alpha , prostaglandin E 2 ( PGE 2 ) and soluble CD 40 ligand ( sCD40L ) , and different combinations thereof are frequently employed for DC activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Experiments were also carried out to determine the levels of interferon gamma ( IFN gamma ) and interleukin ( IL ) 4 produced by splenocytes from nave recipients following the adoptive transfer , and CD 40 ligand ( CD40L ) mediated IL 10 production by DCs from allergic and control mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have compared soluble human trimeric CD 40 ligand ( sCD40L ) combined with IFN gamma , poly ( 1 : C ) , a cocktail of cytokines ( IL 1beta , IL 6 and tumor necrosis factor alpha ) with prostaglandin E ( 2 ) and lipopolysaccharide . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 80 and CD 86 antigen expression was upregulated on B cells but not T cells during incubation with trimeric human CD 40 ligand ( huCD40LT ) + IL 2 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased soluble CD 40 ligand levels in cystic fibrosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In these studies , we demonstrate that exogenous soluble LIGHT or soluble CD 40 ligand ( CD40L ) can promote monocyte derived dendritic cell maturation in vitro by the up regulation of CD 86 , CD 80 , CD 83 , and HLA DR antigen expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using different polymerized forms of CD 40 ligand , we demonstrate that the strength and persistence of CD 40 signaling can induce either function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The effect of elevated serum soluble CD 40 ligand on the prognostic value in patients with acute coronary syndromes . ^^^ We evaluated the clinical predictive value of increased serum soluble CD 40 ligand ( CD40L ) in patients with acute coronary syndromes ( ACS ) and acute chest pain . ^^^ METHODS : Serum levels of soluble CD 40 ligand were measured by ELISA in 128 patients with ACS and in 68 patients with acute chest pain . ^^^ RESULTS : The levels of soluble CD 40 ligand were increased in 57 . 8 % patients with ACS ( > 8 . 0 ng / ml ) and in 35 patients with acute chest pain ( > 8 . 0 ng / ml ) , respectively . ^^^ The level of soluble CD 40 ligand was slightly correlated with measured levels of troponin T ( r=0 . 21 , p < 0 . 05 ) , and the increased soluble CD40L levels ( > 8 . 0 ng / ml ) were associated with higher risk for AMI , sudden death and recurrent angina . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Several large scale clinical studies demonstrate that markers of inflammation , such as high sensitivity C reactive protein ( hsCRP ) , fibrinogen , and soluble CD 40 ligand , are potent and independent predictors of vascular risk . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The adapter molecule , CFm40L , consists of CAR fused to mouse CD 40 ligand via a trimerization motif . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of the p 38 mitogen activated protein kinase , extracellular signal regulated kinase , and phosphoinositide 3 OH kinase signal transduction pathways in CD 40 ligand induced dendritic cell activation and expansion of virus specific CD8+ T cell memory responses . ^^^ CD 40 ligand ( CD40L , CD 154 ) is one of the most potent maturation stimuli for immature DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ectopic CD 40 ligand expression on B cells triggers intestinal inflammation . ^^^ In this study we present evidence that B cells can trigger intestinal inflammation using transgenic ( Tg ) mice expressing CD 40 ligand ( CD40L ) ectopically on B cells ( CD40L / B Tg ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human bone marrow megakaryocytes and platelets express PPARgamma , and PPARgamma agonists blunt platelet release of CD 40 ligand and thromboxanes . ^^^ Platelets are essential not only for clotting , but have an emerging role in inflammation in part due to their release or production of the proinflammatory and proatherogenic mediators CD 40 ligand ( CD40L ) and thromboxanes ( TXs ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand , soluble P selectin , interleukin 6 , and tissue factor in diabetes mellitus : relationships to cardiovascular disease and risk factor intervention . ^^^ BACKGROUND : High levels of the soluble fragment of CD 40 ligand ( sCD40L ) have previously been associated with adverse cardiovascular outcomes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To study the mechanism of this inhibition , we have investigated which immunoglobulin gene regulatory regions might be affected by IL 4 and CD 40 Ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The lack of beta 2 AR induced effect on IL 12 production was due to a beta 2 AR induced inhibition of an IL 18 elicited upregulation of both CD 40 and CD 40 ligand ( CD40L / CD154 ) expressions on monocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Whereas lipopolysaccharide ( LPS ) and B cell activation factor ( BAFF ) mainly activate the type 1 or type 2 pathways , respectively , CD 40 ligand ( CD40L ) strongly activates both . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Adenovirus CD 40 ligand gene therapy counteracts immune escape mechanisms in the tumor Microenvironment . ^^^ We have studied the immunological effects of introducing CD 40 ligand ( CD40L ) , a potent dendritic cell activation molecule , into the tumor micromilieu by adenoviral gene transfer . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand stimulation inhibits the proliferation of mantle cell lymphoma lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We generated EBV specific CTLs that were transgenic for the adenoviral E 1 gene under the control of the cell activation dependent CD 40 ligand ( CD40L ) promoter . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DCs generated in a medium with HCV negative plasma and treated with a defined cocktail of cytokines or a CD 40 ligand trimer matured fully , as measured by the induction of CD 83 expression and the upregulation of costimulatory molecules . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The orally immunized T cells use IL 4 and IL 10 ( but not CD 40 ligand ) to ' educate ' dendritic cells , which in turn induce naive T cells to produce the same cytokines as those produced by the orally immunized memory T cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After stimulation with CD 40 ligand ( CD40L ) , LCs and MoLCs acquired CD 83 and increased CD 86 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recently we reported that high potency ( CMRF 44 ( + ) ) dendritic cells ( DC ) in the peripheral blood of patients with MM failed to significantly up regulate the expression of the B 7 co stimulatory molecules , CD 80 and CD 86 , in response to an appropriate signal from soluble trimeric human CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
One of these of particular interest is soluble CD 40 ligand , a biomarker that may not only indicate active inflammation and platelet activation associated with ACS , but may also exhibit direct prothrombotic properties that mediate early atherogenesis , plaque rupture , and thrombosis . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Such efficiency decreased when MDC are matured with the treatment of IL 4 , CpG oligodeoxynucleotide , or CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Prothrombotic factors including fibrinogen , tissue factor ( TF ) , soluble CD 40 ligand ( sCD40L ) , and homocysteine were decreased by 66 , 27 , 16 , and 22 % , respectively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , we demonstrate that , in primary T cells , dominant negative PAK 2 prevented anti CD3 / CD28 induced IL 2 production , and TCR induced CD 40 ligand expression , both key functions of activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Type A CpG ODN and CD 40 ligand induced pDC to produce large amounts of type 1 IFN , but did not promote maturation . ^^^ CpG ODN and CD 40 ligand , but not influenza virus , induced IL 12p40 and IL 6 secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Following transfection , ligation of CD 40 with soluble CD 40 ligand resulted in a significant increase in VEGF transcriptional activation , and the inhibitory mutant of Ras blocked this CD 40 induced VEGF overexpression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Constitutive expression of CD 40 ligand on HMC 1 surface was not altered by NECA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand and platelets : self perpetuating pathogenic loop in thrombosis and inflammation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functional modulating effect of CD 40 ligand on CD 40 transfected human lung carcinomas ] . ^^^ OBJECTIVE : To determine the modulating effect of CD 40 ligand ( CD40L , CD 154 ) on CD 40 transfected human lung carcinomas and to assess the potential of CD 40 as a therapeutic target . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro generation of DC was achieved using a cytokine cocktail containing tumor necrosis factor alpha , granulocyte macrophage colony stimulating factor , c kit ligand , and soluble CD 40 ligand ; yet , addition of the HDAC inhibitor ( Hdi ) trichostatin A enhanced DC differentiation with retention of the fusion transcripts . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 receptor ( CD 40 ) and its counterpart , the CD 40 ligand ( CD40L / CD154 ) , were originally found to regulate T cell dependent B cell differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NF kappaB 2 p100 processing has recently been shown to be stimulated by a subset of NF kappaB inducers , including lymphotoxin beta , B cell activating factor and CD 40 ligand , via a novel signalling pathway . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 1 beta , IL 6 ) , C reactive protein , CD 40 ligand and adhesion proteins , enhanced fibrinolysis mainly due to decrease in PAI 1 production , and attenuation of thrombin generation mainly indicated by decreased TF expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD 40 ligand in systemic lupus erythematosus . ^^^ CD 40 ligand ( CD40L , also known as CD 154 or gp 39 ) is a member of the tumor necrosis superfamily of transmembrane proteins . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
HIV 1 infected dendritic cells up regulate cell surface markers but fail to produce IL 12 p 70 in response to CD 40 ligand stimulation . ^^^ Here , we examined the ability of HIV 1 infected , in vitro derived DCs to respond to CD 40 ligand ( CD40L ) stimulation with the aim to study events during early HIV 1 infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS AND RESULTS : Intravenous injection of the NO synthase inhibitor N ( G ) nitro L arginine methyl ester in wild type ( WT ) mice significantly reduced platelet vasodilator stimulated phosphoprotein ( VASP ) phosphorylation and increased platelet surface expression of P selectin , CD 40 ligand , and fibrinogen platelet binding , demonstrating that NO production exerts tonic inhibition of platelet activation in mice . ^^^ P selectin , CD 40 ligand expression , and fibrinogen binding were increased in diabetic WT mice but remained unchanged compared with controls in endothelial targeted GCH Tg mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We assessed several thrombophilia markers as well as circulating total and endothelial microparticles ( TMP / EMP ) and soluble CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand . ^^^ BACKGROUND : CD 40 ligand ( CD40L ) expression on platelets is mediated by agonists , but the underlying mechanism is still unclear . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of atorvastatin on risk of recurrent cardiovascular events after an acute coronary syndrome associated with high soluble CD 40 ligand in the Myocardial Ischemia Reduction with Aggressive Cholesterol Lowering ( MIRACL ) Study . ^^^ BACKGROUND : Patients with acute coronary syndromes have elevated plasma levels of the proinflammatory , prothrombotic cytokine CD 40 ligand ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Trans splicing repair of CD 40 ligand deficiency results in naturally regulated correction of a mouse model of hyper IgM 10 linked immunodeficiency . 10 linked immunodeficiency with hyper IgM ( HIGM 1 ) , characterized by failure of immunoglobulin isotype switching , is caused by mutations of the CD 40 ligand ( CD40L ) , which is normally expressed on activated CD 4 ( + ) T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Use of anti CD 40 ligand monoclonal antibody as antirejection therapy in a murine peripheral nerve allograft model . ^^^ Monoclonal antibody directed against CD 40 ligand prevents acute allograft rejection in several models of solid organ transplantation . ^^^ This study describes the use of CD 40 ligand as antirejection therapy in a mouse peripheral nerve allograft model . ^^^ Treated animals ( n = 11 ) received anti CD 40 ligand antibody applied to the graft and by intraperitoneal injections postoperatively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of atorvastatin on the expression of CD 40 ligand and P selectin on platelets in patients with hypercholesterolemia . ^^^ The effects of atorvastatin on levels of the CD 40 ligand ( CD40L or CD 154 ) and P selectin on platelets were investigated in patients with hypercholesterolemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Surface expression of collagen receptor Fc receptor gamma / glycoprotein 6 is enhanced on platelets in type 2 diabetes and mediates release of CD 40 ligand and activation of endothelial cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immature 16+mDC and 16 mDC ( iDC ) obtained by culture of purified monocytes with GM CSF and IL 4 were maturated with ( 1 ) Toll like receptor ( TLR ) ligands [ lipopolysaccharide ( LPS ) ] , ( 2 ) lymphocyte derived ( soluble CD 40 ligand , IFN gamma ) and ( 3 ) endogenous inflammatory stimuli [ TNF alpha , prostaglandin ( PG ) E 2 ] . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The inability of mDCs to secrete IL 12 was maintained , even when they were restimulated by the activated T cell signal CD 40 ligand in the absence of cmvIL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand : a novel target in the fight against cardiovascular disease . ^^^ OBJECTIVE : To discuss the role of CD 40 ligand ( CD40L ) in atherosclerosis and acute coronary syndromes ( ACS ) , as well as describe relevant clinical literature evaluating the effects of pharmacotherapeutic agents on CD40L expression and soluble CD40L levels . ^^^ DATA SOURCES : A MEDLINE and EMBASE search ( 1966 September 2003 ) was conducted using the key terms CD 40 , CD 40 ligand , platelets , inflammation , and drug therapy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) was shown to be a promising molecule for immunotherapy of B CLL playing a critical role in immune activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand rescues inhibitor of differentiation 3 mediated G 1 arrest induced by anti IgM in WEHI 231 B lymphoma cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CONCLUSIONS / INTERPRETATION : Interaction between activated T lymphocytes expressing CD 40 ligand and duct cells expressing CD 40 may contribute to the immune responses involved in Type 1 diabetes mellitus and islet graft rejection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand in pulmonary arterial hypertension : possible pathogenic role of the interaction between platelets and endothelial cells . ^^^ CD 40 ligand ( L ) may promote inflammation and thrombus formation , and we hypothesized that CD40L could be involved in the pathogenesis of PAH . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand , a 33 kDa cell membrane molecule , a member of the tumor necrosis factor superfamily , is an important costimulatory molecule during immune response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand ( CD40L ) CD 40 interaction is important in regulating the cellular immune response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Fetal bradycardia following intrauterine platelet transfusion : might elevated levels of donor soluble CD 40 ligand play a role . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Coexpression of GM CSF and IL 4 in CD14+ cells was sufficient to induce their differentiation into a DC like phenotype , as evidenced by their morphology , immature immunophenotypic profile ( CD 14 , CD1a+ , CD80+ , CD86+ , MHC I+ , MHC II+ ) , and their ability to further develop into a mature phenotype ( CD83+ ) on further treatment with soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The most common HIGM syndrome is 10 linked and due to mutations of CD 40 ligand ( CD40L ) expressed by activated CD 4 ( + ) T lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
RFs from synovium derived LCLs efficiently induced B cell activation , and the addition of CD 40 ligand had a synergistic effect . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Release of soluble CD 40 ligand after platelet activation : studies on the solubilization phase . sCD40L is released from platelets as a soluble , proteolyzed form of CD 40 ligand ( CD40L ; CD 154 ) which is exposed on the surface after platelet activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The levels of soluble CD 40 ligand and soluble P selectin increased up to day 5 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Temporary deterioration of liver injury was observed in 2 CSP positive boys with CD 40 ligand deficiency ( CD40LD ) who were undergoing nonmyeloablative hematopoietic stem cell transplantation ( HSCT ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : CD 40 ligand ( CD 154 ) expressed on activated T helper cells is a key costimulatory molecule for antigen presenting cells expressing the corresponding receptor CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We first determined that the combination of interleukin ( IL ) 1beta , IL 3 , IL 7 , tumor necrosis factor alpha , stem cell factor and CD 40 ligand was optimal for generating dendritic like cells from t ( 9 ; 22 ) ALL cell lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The hyper immunoglobulin M ( IgM ) syndrome ( HIGM ) , characterized by recurrent infections , low serum IgG and IgA , normal or elevated IgM , and defective class switch recombination and somatic hypermutation , is a heterogenous disorder with at least 5 distinct molecular defects , including mutations of the genes coding for the CD 40 ligand ( CD40L ) and IKK gamma ( NEMO ) genes , both 10 linked ; and mutations of CD 40 , activation induced cytidine deaminase ( AICDA ) , and uracil DNA glycosylase ( UNG ) , associated with autosomal recessive HIGM syndromes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand gene and Kawasaki disease . ^^^ A recent report of elevated expression of CD 40 ligand ( CD40L ) , which maps to Xq 26 , during the acute phase KD , and its relationship to the development of coronary artery lesions ( CAL ) prompted us to screen for polymorphism of CD40L and to study the association of the gene to KD . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon incubation with inflammatory cytokines such as tumor necrosis factor alpha and interleukin ( IL ) 1beta or maturating agents ( lipopolysaccharide , CD 40 ligand ) , FcalphaRI and TfR expression on Mo DC was specifically up regulated , whereas FcgammaRs and FcepsilonRI expression was down regulated . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased CD 40 ligand and platelet monocyte aggregates in patients with type 1 diabetes mellitus . ^^^ We investigated whether CD 40 ligand ( L ) expression and platelet monocyte aggregation are increased in patients with type 1 diabetes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The transcription factor analysis revealed distinct signaling pathways operated by CD 40 reactive NAbs compared to those by CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To test the effect of activating islet associated APCs in situ , we generated transgenic mice expressing CD 154 ( CD 40 ligand ) under control of the rat insulin promoter ( RIP ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Pharmacological immunosuppression for GVHD prophylaxis and therapy , including unspecific approaches with corticosteroids or methotrexate ( MTX ) , as well as more specific therapy with cyclosporin A ( CsA ) , tacrolimus ( FK 506 ) , sirolimus , mycophenolate mofetil ( MMF ) , antithymocyte globulin ( ATG ) , and monoclonal antibodies ( MAbs ) directed against CD 3 , CD 25 , CD 52 , cytotoxic T lymphocyte antigen ( CTLA ) 4 , CD 40 ligand , or TNF alpha , have been proven to be effective . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The addition of cytokines alone , such as GM CSF or CD 40 ligand , did not affect the expression of CD 83 in untreated NB 4 cells but they up regulated CD 83 in ATRA treated cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : Replication defective adenoviruses ( Ad ) were constructed expressing the hyperfusogenic version of the GALV glycoprotein linked to a blocking ligand ( C terminal extracellular domain of CD 40 ligand ) through either an MMP cleavable linker ( AdM 40 ) or a non cleavable linker ( AdN 40 ) . ^^^ Infection of these cell lines with AdN 40 did not result in fusion or cytotoxic cell death , despite the presence of infection , as demonstrated by GFP positivity , therefore indicating that the displayed CD 40 ligand blocked GALV induced fusion . ^^^ Western immunoblotting demonstrated the presence of the cleaved CD 40 ligand in the supernatant of fused glioma cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We used unpulsed native CLL cells and CD 40 ligand ( CD40L ) stimulated CLL cells as antigen presenting cells ( APCs ) to expand autologous T cells from 13 patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we show that although the induction of IkappaB degradation in lymphocytes by TNF is independent of NIK , its induction by CD 70 , CD 40 ligand , and BLyS / BAFF , which all also induce NF kappaB2 / p100 processing , does depend on NIK function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Association between enhanced soluble CD 40 ligand and prothrombotic state in inflammatory bowel disease . ^^^ The aim of this study was to investigate the role of the soluble CD 40 ligand ( sCD40L ) , which displays prothrombotic properties , in patients with ulcerative colitis ( UC ) and Crohn ' s disease ( CD ) in comparison with inflammatory and healthy controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This was followed by up regulated expression of receptor activator of nuclear factor kappa B ligand ( RANKL ) and CD 40 ligand ( CD40L on days 28 and 70 in infected interleukin 10 ( / ) mice only . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , activation of DC by immune stimuli ( CD 40 ligand ) did not regulate NADPH oxidase components or respiratory burst . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Potential use of CD 40 ligand for immunotherapy of childhood B cell precursor acute lymphoblastic leukaemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activated T cells demonstrated enhanced CD 40 ligand ( CD40L ) expression within 72 hours of stimulation , which stimulated other cells to secrete IL 12 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NF kappaB elements associated with the Stat 6 site in the germline gamma 1 immunoglobulin promoter are not necessary for the transcriptional response to CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Atm / cells secreted substantially less IgA , IgG 1 , IgG2a , IgG 3 , and IgE than wild type ( WT ) controls in response to stimulation with lipopolysaccharide , CD 40 ligand , or anti IgD plus appropriate cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) plays an important role in DC activation / maturation but is not available for clinical applications . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro EBV infected subline of KMH 2 , derived from Hodgkin lymphoma , expresses only EBNA 1 , while CD 40 ligand and IL 4 induce LMP 1 but not EBNA 2 . ^^^ Importantly , exposure to CD 40 ligand and IL 4 induced LMP 1 without EBNA 2 expression and lytic replication . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 ligand in CD 40 positive murine tumors activates transcription of the interleukin 23 subunit genes and produces antitumor responses . ^^^ CD 40 positive dendritic cells ( DCs ) are stimulated with CD 40 ligand ( CD40L ) and subsequently secrete a number of cytokines including interleukin ( IL ) 23 , which is involved in cell mediated immune responses . ^^^ Expression of CD 40 ligand ( CD40L ) on tumors can activate host immune systems and produce antitumor effects against the tumors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Biological effects of soluble CD 40 ligand on lung cancer cell line A 549 and its mechanism . ] . ^^^ This study was to investigate biological effects of soluble CD 40 ligand ( sCD44L ) on lung cancer cell line A 549 ( CD 40 positive ) , and its possible mechanism . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is an accessory signal for T cell activation and can overcome T cell anergy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Measurement of soluble P selectin and soluble CD 40 ligand in serum and plasma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Levels of tumor necrosis factor alpha ( TNF alpha ) , interleukin 6 ( IL 6 ) , interleukin 1 receptor antagonist ( IL 1 ra ) and soluble CD 40 ligand ( sCD40L ) were higher in ACS patients as compared to controls and remained elevated up to day 120 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It has been revealed that TNF receptor associated factor 6 ( TRAF 6 ) , a signaling adapter molecule common to the IL 1R / TLR family and TNFR superfamily , is important not only for DC maturation , cytokine production , and T cell stimulatory capacity of DCs in response to TLR ligands ( e . g . lipopolysaccharide ) or CD 40 ligand , but also for the homeostasis of splenic DC subsets . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Intratumoral administration of immature dendritic cells following the adenovirus vector encoding CD 40 ligand elicits significant regression of established myeloma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The goal of this study was to investigate whether 1 ) depressed post MI patients have higher markers of platelet activation as measured by plasma levels of beta thromboglobulin ( betaTG ) , platelet factor 4 ( PF 4 ) and soluble CD 40 ligand ( sCD40L ) and higher serotonin ( 5 HT ) levels than non depressed post MI patients and 2 ) treatment with the antidepressant mirtazapine decreases platelet activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Newer markers such as CD 40 ligand appear also to provide important information regarding risk stratification . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , we show that patient B cells stimulated with CD 40 ligand are impaired in both p 65 and c Rel activation , and whereas addition of IL 4 can enhance p 65 activity , c Rel activity remains deficient . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B Lymphocytes activated by CD 40 ligand induce an antigen specific anti tumour immune response by direct and indirect activation of CD 8 ( + ) T cells . ^^^ In this report , we describe the ability of CD 40 ligand ( CD40L ) activated , antigen loaded B cells to initiate antigen specific anti tumour immune responses in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand is an early initiator of inflammation after coronary intervention . ^^^ The role of soluble CD 40 ligand ( sCD40L ) as a potential initiator of inflammation after stenting has not been described . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Efficient gene transfer of CD 40 ligand into ovarian carcinoma cells with a recombinant adeno associated virus vector . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Preliminary findings from comparisons of wild type , CD 40 ligand knock out , and CD 28 knock out C57BL / 6 mice strongly support the suggestion of a critical role for T cell antigen presenting cell interactions in the immune alterations observed in chronic ethanol abuse . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : The authors have previously demonstrated that inhibition of CD 28 and CD 40 ligand ( CD40L ) co stimulatory signals by adenovirus mediated cytotoxic T lymphocyte associated ( CTL ) antigen 4 ( A 4 ) immunoglobulin ( Ig ) and CD40Ig gene therapies induces tolerance or long term acceptance in rat liver and heart allograft transplantation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Moreover , a variety of pro ( soluble CD 40 ligand , placental growth factor , interleukin 6 , pregnancy associated plasma protein A , myeloperoxidase , monocyte chemoattractant protein 1 ) and anti inflammatory markers ( interleukin 10 , activin A ) have been suggested to provide relevant prognostic information in patients with acute coronary syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Analysis of serum soluble CD 40 ligand ( sCD40L ) in the patients undergoing allogeneic stem cell transplantation : platelet is a major source of serum sCD40L . ^^^ CD 40 ligand ( CD40L ) is expressed not only on activated T cells but also on activated platelets . ^^^ A soluble CD 40 ligand ( sCD40L ) is released from the activated T cells and platelets by ill defined proteolytic process in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mutational screening of the CD 40 ligand ( CD40L ) gene in patients with 10 linked hyper IgM syndrome ( XHIM ) and determination of carrier status in female relatives . ^^^ AIMS : To analyse the gene encoding the CD 40 ligand ( CD40L ) in 11 Australian patients from 10 unrelated families with the 10 linked hyper IgM ( XHIM ) phenotype . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : In the present study , we generated a novel fusion hybrid DC / J558 ( CD40L ) by fusing DCs and an engineered J 558 ( CD40L ) myeloma cells expressing CD 40 ligand ( CD40L ) molecule using polyethylene glycol ( PEG ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand enhances monocyte tissue factor expression and thrombin generation via oxidative stress in patients with hypercholesterolemia . ^^^ OBJECTIVES : We tested the hypothesis that CD 40 ligand ( CD40L ) induces a prothrombotic state by enhancing oxidative stress . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Construction of recombinant eukaryotic expression plasmid containing murine CD 40 ligand gene and its expression in H 22 cells . ^^^ AIM : To construct a recombinant murine CD 40 ligand ( mCD40L ) eukaryotic expression vector for gene therapy and target therapy of hepatocellular carcinoma ( HCC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The efficacy of CD 40 ligand blockade in discordant pig to rat islet xenotransplantation is correlated with an immunosuppressive effect of immunoglobulin . ^^^ BACKGROUND : The authors ' aim was to evaluate the efficacy of immunosuppression with monoclonal anti CD 40 ligand antibodies ( aCD40L ) or nonspecific polyclonal intravenous immunoglobulin ( IVIG ) in the pig to rat islet xenotransplantation model . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the effects of abciximab , eptifibatide and no GPIIb IIIa antagonist ( control ) on soluble CD 40 ligand ( sCD40L ) and the formation of leukocyte platelet aggregates ( LPA ) in 98 ACS patients undergoing percutaneous coronary intervention ( PCI ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , activation with CpG or CD 40 ligand profoundly up regulated AhR mRNA and protein . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Short term treatment with atorvastatin reduces platelet CD 40 ligand and thrombin generation in hypercholesterolemic patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To examine a possible association between the smallpox vaccine and postvaccination ischemic events , we investigated alterations in levels of prothrombotic proteins ( plasminogen activator inhibitor type 1 [ PAI 1 ] and soluble CD 40 ligand [ sCD40L ] ) in recently vaccinated individuals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effects of tirofiban and statins on high sensitivity C reactive protein , interleukin 6 , and soluble CD 40 ligand following percutaneous coronary interventions in patients with stable coronary artery disease . ^^^ This study assessed the effects of tirofiban and statins on high sensitivity C reactive protein , interleukin 6 , and soluble CD 40 ligand after percutaneous coronary intervention in patients who had stable coronary artery disease . ^^^ Tirofiban insignificantly limited the increase of soluble CD 40 ligand after revascularization , especially in patients who had high levels of this marker at baseline ( p = 0 . 06 ) , whereas statins significantly inhibited increases in interleukin 6 and , to a lesser extent , high sensitivity C reactive protein without affecting the soluble CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate the potential role of LCs in scrapie transportation from the skin , we utilized mouse models in which their migration was blocked either due to CD 40 ligand deficiency ( CD40L / mice ) or after caspase 1 inhibition . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of B cells , co stimulated with IL 2 , IL 10 and soluble CD 40 ligand ( sCD40L ) with different CpG ODNs , had differing effects on the terminal differentiation in vitro of B cells into immunoglobulin secreting cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : Activated platelets participate in inflammatory and microbicidal processes by upregulation of surface selectins , shedding of CD 40 ligand , and release of platelet microbicidal proteins and microparticles . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Remission of proliferative lupus nephritis following B cell depletion therapy is preceded by down regulation of the T cell costimulatory molecule CD 40 ligand : an open label trial . ^^^ As early as 1 month from baseline , the expression of the costimulatory molecule CD 40 ligand on CD4+ T cells was decreased by 4 fold , and it was almost blocked when partial remission was clinically evident . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased plasma soluble CD 40 ligand concentrations in systemic sclerosis and association with pulmonary arterial hypertension and digital ulcers . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Quantitative mRNA analysis revealed that IL 4 transduced DCs stimulated with CD 40 ligand expressed higher levels of IL 12p35 mRNA , but lower levels of mRNA for IL 23p19 and the common subunit p 40 found in both IL 12 and IL 23 , compared with control DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand in acute and chronic heart failure . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this report we show that CD 40 ligand , another member of the TNF superfamily , also triggers the activation of these signalling pathways but , importantly , utilises only the PI 3 kinase cascade for anti apoptotic responses , inasmuch as suppression of PI 3 kinase but not NF kappaB sensitises carcinoma cells to CD40L induced apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A semi quantitative RT PCR method was used to determine intracellular adhesion molecule 1 ( ICAM 1 ) , vascular cell adhesion molecule 1 ( VCAM 1 ) , lymphocyte function associated antigen ( LFA 1 ) , LFA 3 , CD 40 , CD 40 ligand ( CD40L ) and RANTES ( regulated upon activation , normal T cell expressed and secreted ) gene expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVES : We and others have shown that B cell precursor acute lymphoblastic leukemia cells ( ALL ) stimulated with CD 40 ligand become efficient antigen presenting cells ( APC ) capable of expanding autologous , tumor specific T cells from patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Human TSLP promotes CD 40 ligand induced IL 12 production by myeloid dendritic cells but maintains their Th 2 priming potential . ^^^ Here we show that TSLP primes DCs to produce large amounts of IL 12 after CD 40 ligand stimulation , similar to IL 4 priming of DCs . ^^^ In contrast to IL 4 priming , DCs activated with TSLP and CD 40 ligand induce the differentiation of naive CD 4 ( + ) T cells into effectors producing both Th 1 and Th 2 cytokines , a unique profile that is reminiscent of the late phase of allergy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand dependent induction of COX 2 gene expression in endothelial cells by activated platelets : inhibitory effects of atorvastatin . ^^^ CD 40 ligand surface expression of platelets was tested by fluorescence activated cell sorting analyses . ^^^ Co incubation of platelets with atorvastatin was shown to reverse this up regulation via reduction of CD 40 ligand surface expression on platelets . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand : not bad to the bone ( marrow ) , after all . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The induced expression , by anti CD 3 mAb , of CTLA 4 , CD 25 , and CD 40 ligand ( CD40L ) was decreased following incubation of T cells with gingipains which also led to decreased response to anti CD 3 and anti CD 28 mAbs as shown by reduction of interleukin 2 ( IL 2 ) production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thyroid cancers express CD 40 and CD 40 ligand : cancers that express CD 40 ligand may have a greater risk of recurrence in young patients . ^^^ Although the factors that control this are unknown , CD 40 and CD 40 ligand might be important . ^^^ To test this , we stained 36 papillary ( PTC ) and four follicular ( FTC ) thyroid carcinomas for CD 40 ( n = 37 ) and CD 40 ligand ( n = 36 ) and graded staining from absent ( grade 0 ) to intense ( grade 3 ) . ^^^ Follicular cells of the majority of thyroid tumors expressed CD 40 ( 30 / 37 , 81 % ) and CD 40 ligand ( 15 / 24 , 69 % ) . ^^^ Among young patients , CD 40 ligand expression was more intense in multifocal ( 1 . 1 + / 0 . 2 vs . 0 . 45 + / 0 . 2 , p = 0 . 037 ) , aggressive ( 1 . 14 + / 0 . 14 vs . 0 . 65 + / 0 . 2 , p = 0 . 05 ) and recurrent tumors ( 1 . 2 + / 0 . 2 vs . 0 . 65 + / 0 . 2 , p = 0 . 05 ) and associated with reduced disease free survival ( p = 0 . 03 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Previously , we reported that allogeneic skin grafts were rapidly rejected by CD 28 and CD 40 ligand double deficient mice mediated by CD8+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Simultaneous LFA 1 and CD 40 ligand antagonism prevents airway remodeling in orthotopic airway transplantation : implications for the role of respiratory epithelium as a modulator of fibrosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD 154 ) takes part in regulation of the transition to mononuclear cell dominance during peritonitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Such an effect was less remarkable when DC was stimulated by a physiological stimulus , CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the serum level of soluble CD 40 ligand ( sCD40L ) in coronary heart disease and its relationship with serum lipid levels . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : Splenic B cells from BALB / c mice were stimulated with anti CD 40 mAb and IL 4 or with soluble CD 40 ligand in the presence or absence of SP 600125 , an anthrapyrazolone inhibitor of JNK . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DESIGN , SETTING , AND PATIENTS : Measurement of PlGF levels as well as levels of markers of myocardial necrosis ( troponin T [ TnT ] ) , platelet activation ( soluble CD 40 ligand [ sCD40L ] ) , and inflammation ( high sensitivity C reactive protein [ hsCRP ] ) in an inception cohort of 547 patients with angiographically validated ACS participating in the CAPTURE ( c7E3 Fab Anti Platelet Therapy in Unstable Refractory Angina ) trial and in a heterogeneous cohort of 626 patients presenting with acute chest pain to an emergency department in Germany between December 1996 and March 1999 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Nonreplicating recombinant vaccinia virus expressing CD 40 ligand enhances APC capacity to stimulate specific CD4+ and CD8+ T cell responses . ^^^ To further investigate the capacity of these vectors to provide ligands for different costimulatory pathways relevant in the generation of T cell responses , we constructed a recombinant virus ( rVV ) expressing CD 40 ligand or CD 154 ( CD154rVV ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In correspondence , minocycline decreased the expression on T cells of the CD 40 ligand ( CD40L ) , a key molecule regulating the contact mediated interaction of T cells with microglia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this paper , we discuss biomarkers implicated in the inflammatory process leading to atherothrombosis , including CRP , adiponectin , monocyte chemoattractant protein 1 ( MCP 1 ) , CD 40 ligand and lipoprotein associated phospholipase A ( 2 ) ( Lp PLA ( 2 ) ) , and the effect of statins on these markers and their potential relationship to cardiovascular events . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , CDK4R24C transductants showed a marked increase in apoptotic susceptibility to membrane presented CD 40 ligand , being intermediate between normal cells ( nonsusceptible ) and transformed cells ( highly susceptible ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Study of plasma levels of soluble CD 40 ligand in systemic lupus erythematosus patients who have undergone plasmapheresis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immune neutralization of the CD 40 ligand ( CD 154 ) at the time of challenge significantly reduced accumulation of Th 1 effectors and injury . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The effect of prostaglandins E 1 and E 2 on the 1 ng / ml lipopolysaccharide induced expression of intercellular adhesion molecule ( ICAM ) 1 , B7 . 1 , B7 . 2 , CD 40 and CD 40 ligand ( CD40L ) on monocytes was examined . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Conservation of biological properties of the CD 40 ligand , CD 154 in a non mammalian vertebrate . ^^^ Signals delivered by the CD 40 ligand , CD 154 , have crucial roles in immune responses in mammals , being required for development of germinal centres , maturation of T dependent antibody responses , and generation of B cell memory . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Enhanced soluble CD 40 ligand contributes to endothelial cell dysfunction in vitro and monocyte activation in patients with diabetes mellitus : effect of improved metabolic control . ^^^ Soluble CD 40 ligand ( sCD40L ) is enhanced in diabetes ; however , the molecular mechanisms linking sCD40L to accelerated atherosclerosis in diabetes are still unclear . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The hyper IgM syndrome ( HIM ) is a rare primary immunodeficiency disorder caused by defects in the CD 40 ligand ( CD40L ) / CD40 signaling pathway . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interleukin ( IL ) 18 , which is elevated in plasma during the acute rejection after organ transplantation , elicits the expression of intercellular adhesion molecule ( ICAM ) 1 , B7 . 1 , B7 . 2 , CD 40 , and CD 40 ligand ( CD40L ) on monocytes as well as the production of interferon ( IFN ) gamma and IL 12 and proliferation of T cells during the human mixed lymphocyte reaction ( MLR ) in an in vitro model of acute rejection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand increases complement C 3 secretion by proximal tubular epithelial cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Signaling through CD 40 ligand decreases CD 80 expression on murine Langerhans cells and enhances IL 12 p 40 production . ^^^ We previously showed that murine Langerhans cells ( LC ) express CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this review we have analyzed different inflammatory markers currently used , such as C reactive protein ( CRP ) , CD 40 ligand , adhesion molecules and chemokines , and their possible modulation by therapeutic intervention with 3 hydroxy 3 methylglutaryl coenzyme A ( HMG CoA ) reductase inhibitors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The current studies demonstrate that CD 40 and CD 40 ligand ( CD40L ) , two co stimulatory immune molecules , are involved in TD induced selective neuronal death . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
MAIN OUTCOME MEASURES : Fasting blood ( 50 ml ) was obtained , and the following parameters were assayed : high sensitivity CRP , fibrinogen , lipid profile , insulin , whole blood cytokines after stimulation with lipopolysaccharide ( LPS ; 100 ng / ml for 24 h ) , soluble cell adhesion molecules , plasminogen activator inhibitor 1 , CD 40 , CD 40 ligand , leptin , adiponectin , monocyte chemoattractant protein 1 , IL 8 , matrix metalloproteinase 3 ( MMP 3 ) , and MMP 9 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This migration pattern correlated with upregulation of integrin alpha4beta7 and CD 154 ( CD 40 ligand ) on T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
New markers , such as ischemia modified albumin ( IMA ) and CD 40 ligand , and new technologies , such as proteomics , are under investigation to advance our knowledge of heart disease . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Patients with chronic lymphocytic leukemia ( CLL ) treated with adenovirus CD 154 ( Ad CD 154 , CD 40 ligand [ CD40L ] ) gene therapy experienced rapid reductions in leukemia cell counts and lymph node size associated with the induced expression of Fas ( CD 95 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand measurement inaccuracies attributable to specimen type , processing time , and ELISA method . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Vitamin C inhibits platelet expression of CD 40 ligand . ^^^ Upon stimulation with agonists , platelets express CD 40 ligand ( CD40L ) , a transmembrane protein implicated in the initiation and progression of atherosclerotic disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Sustained elevation of serum CD 40 ligand levels one month after coronary angioplasty predicts angiographic restenosis . ^^^ RESULTS : Levels of soluble CD 40 ligand ( sCD40L ) and matrix metalloproteinase 2 showed a rise and fall pattern over six months , with peak levels measured at one month ( P < 0 . 0001 ) , while levels of soluble vascular cell adhesion molecule 1 increased after angioplasty and remained elevated at six months ( P = 0 . 07 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
C reactive protein , CD 40 ligand , P selectin and IL 6 ) has been shown to correlate with increased risk of atherothrombotic events . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Atherogenesis in mice does not require CD 40 ligand from bone marrow derived cells . ^^^ OBJECTIVE : Recent research suggests a central role for CD 40 ligand ( CD40L ) in atherogenesis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Class switching usually requires engagement of CD 40 on B cells by CD 40 ligand ( CD40L ) on antigen activated CD 4 ( + ) T cells and the production of cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When splenic B cells were stimulated with CD 40 ligand ( CD40L ) and IL 4 , terminal differentiation was induced in the B cells from c fos transgenic ( H 2 c fos ) mice but barely in those from control littermates and from c fos deficient mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IgE inhibition was also observed in cells stimulated by IL 4 / CD40 F ( ab ' ) ( 2 ) or IL 4 plus soluble CD 40 ligand , demonstrating that co cross linking of CD 32 and CD 40 was not necessary to induce inhibition . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased CD 40 ligand in patients with acute anterior uveitis . ^^^ We wanted to evaluate whether the T cell activation marker CD 40 ligand is involved in the AU immunopathogenesis . ^^^ METHODS : We evaluated the expression of the CD 40 ligand on CD4+ T cells , CD8+ T cells and CD19+ B cells on peripheral blood mononuclear cells using flow cytometry in six patients with unilateral AU , six patients with monosymptomatic optic neuritis ( ON ) as inflammatory controls , and in six healthy controls . ^^^ The ex vivo induction of the CD 40 ligand on T cells in patients and controls was also studied . ^^^ RESULTS : A significantly higher expression of the CD 40 ligand on both CD4+ ( p < 0 . 05 ) and CD8+ ( p < 0 . 05 ) T cells in patients with AU compared to ON patients and healthy controls was found . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The discovery that platelets express CD 40 and the CD 40 ligand has transformed these cells , once seen as exclusively involved in coagulation and thrombosis , into active players of immunity and inflammatory injury . ^^^ This occurs either through the constitutive expression of CD 40 on the platelet surface or the activation induced expression of the CD 40 ligand , which is membrane bound and released from the surface in a soluble form . ^^^ Therefore , considering platelet CD 40 and CD 40 ligand as novel biological targets is justified and supported by animal studies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The aim of this study was to determine the immunomodulatory effects of IL 12 , IL 18 and CD 154 ( CD 40 ligand , CD40L ) in DNA vaccination against the classical swine fever virus . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Biochemical and clinical markers to assess therapeutic effects included HbA1c , fasting glucose , insulin , adiponectin , lipids , high sensitivity C reactive protein ( hsCRP ) , intracellular adhesion molecule , vascular cell adhesion molecule , vascular endothelial growth factor , fibrinogen , von Willebrand factor , matrix metalloproteinase ( MMP ) 9 , monocyte chemoattractant protein ( MCP ) 1 , soluble CD 40 ligand , and carotid intima media thickness ( IMT ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 and CD 40 ligand increased with ovariectomy and decreased with 17beta estradiol and CEE treatments more than with raloxifene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this chapter , we describe the design and methods for cloning a cDNA gene coding for the mouse CD40L molecule and for construction of the expression vector pcDNA CD40L , as well as the methods for generation of engineered myeloma cells J558 / CD40L expressing CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Our initial finding that CD 40 and CD 40 ligand ( CD40L ) deficient mice displayed prolonged tail bleeding and platelet function analyzer ( PFA 100 ) closure times prompted us to further investigate the role of the CD 40 CD40L dyad in primary hemostasis and platelet function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We further observed that antithrombin limits CD 40 ligand expression in adenosine diphosphate activated platelets and inhibits the shedding of syndecan 4 from activated platelets . ^^^ We suggest that antithrombin might exert beneficial effects in disseminated intravascular coagulation by reducing platelet activation , observed as inhibited CD 40 ligand expression , syndecan 4 shedding , and adenosine diphosphate and adenosine triphosphate release from activated platelets with subsequent inhibition of neutrophil respiratory burst . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
They also play a pivotal role in the process of inflammation , as demonstrated by the finding that CD 40 ligand is shed from activated platelets to directly initiate inflammation of the vessel wall . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The mechanism of both drugs was independent of platelets as indicated by flow cytometric measurement of change of their expression of P selectin , platelet monocyte aggregates , V / Va and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Unstimulated T cells did not express CD 40 ligand ( CD40L ) , whereas activation with IL 2 led to expression of this costimulatory molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand expression correlates with resolution of peritonitis and mononuclear cell recruitment . ^^^ We have previously demonstrated that CD 40 ligation upregulates the secretion of mononuclear chemokines from peritoneal mesothelial cells ( PMC ) , and that blocking the CD 40 ligand ( CD 154 ) reduced the mononuclear infiltrate in a model of peritonitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
De novo mutation causing 10 linked hyper IgM syndrome : a family study in Taiwan . 10 linked hyper IgM syndrome ( XHIM ) is a rare primary immunodeficiency disorder caused by mutations of the gene encoding the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Percutaneous transluminal mitral valvuloplasty reduces circulating soluble CD 40 ligand in rheumatic mitral stenosis . ^^^ BACKGROUND : Recent data suggest that the pathogenesis of vascular inflammation and thrombosis involves CD 40 ligand ( CD40L ) , which is mostly derived from platelets . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , BALB / c bMSCs had an enhancing effect on CD 40 expression and inhibitory effects on CD 40 ligand ( CD40L ) ectopic hyperexpression on B cells from BXSB mice . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interventions included evaluation of intercurrent disease , ankle and arm blood pressures , plasma glucose and lipid levels , plasma homocysteine , cardiolipin antibodies , resistance to activated protein C , plasma endothelin 1 , and the inflammatory mediators tumor necrosis factor alpha , interleukin 6 , neopterin , high sensitivity C reactive protein , CD 40 ligand , and 8 iso prostaglandin F alpha in plasma . ^^^ The plasma levels of interleukin 6 ( P < . 0001 ) , tumor necrosis factor alpha ( P < . 0001 ) , neopterin ( P < . 0001 ) , and high sensitivity C reactive protein ( P = . 002 ) at admission for critical limb ischemia were all significantly lower in the survivors , whereas there was no difference concerning CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transduction of CLL cells by CD 40 ligand enhances an antigen specific immune recognition by autologous T cells . ^^^ A promising approach is provided by the transduction of CLL cells with CD 40 ligand ( CD40L ) by viral vectors to enhance their immunogenicity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
No studies have attempted to correlate platelet activation status , as measured by soluble plasma markers ( soluble CD 40 ligand , soluble P selectin , soluble glycoprotein 5 ) , with the appearance of diseased coronary arteries . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD4+ T cells and platelets express CD 40 ligand ( CD40L ) and are reported to mediate proinflammatory events in renal proximal tubular epithelial cells ( RPTEC ) via interaction with CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In response to CD 40 ligand and tumor necrosis factor alpha , the cells acquire a mature phenotype of dendritic cells that is characterized by up regulation of human leukocyte antigen ( HLA ) ABC , HLA DR , CD 80 , CD 86 , CD 40 , and CD 54 and appearance of CD 83 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recurrent acalculous cholecystitis and sclerosing cholangitis in a patient with 10 linked hyper immunoglobulin M syndrome . 10 linked hyper immunoglobulin M ( IgM ) syndrome ( XHIGM ) is a rare genetic primary immunodeficiency disease caused by mutations of the CD 40 ligand ( CD40L ) gene with normal or elevated levels of IgM and markedly decreased serum IgG , IgA , and IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Serial changes in circulating concentrations of soluble CD 40 ligand and C reactive protein in patients with unstable angina undergoing coronary stenting . ^^^ BACKGROUND : This study tested the hypothesis that serum concentrations of high sensitivity C reactive protein ( hs CRP ) and soluble CD 40 ligand ( sCD40L ) significantly reflect serial changes in patients with unstable angina , and thus the serum concentrations of these inflammatory biomarkers may be good candidates for predicting late restenosis after coronary stenting . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and tissue factor expression was increased in the ischemic coronary artery and reduced after clopidogrel administration . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transfectants expressing CD 40 ligand and hygromycin phosphotransferase were not more immunogenic than cells expressing hygromycin resistance alone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dipyridamole also suppressed platelet soluble CD 40 ligand release . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Plasma matrix metalloproteinase 9 , tissue inhibitor of metalloproteinase 2 , and CD 40 ligand levels in patients with stable coronary artery disease . ^^^ In addition , high levels of the soluble fragment of CD 40 ligand ( sCD40L ) have previously been associated with adverse cardiovascular outcomes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In sharp contrast to LPS stimulated DCs , pollen treated DCs did not produce cytokines [ interleukin ( IL ) 10 , IL 12 , tumor necrosis factor ( TNF ) alpha ] but retained the ability to secrete high levels of these cytokines upon simulation with soluble CD 40 ligand and interferon ( IFN ) gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Signals that induce human PDC to promote a type 1 response ( i . e . , viruses ) and a type 2 response [ i . e . , CD 40 ligand ( CD40L ) ] also induced PDC isolated from tonsils to secrete chemokines preferentially attracting Th 1 cells [ such as interferon gamma ( IFN gamma ) inducible protein ( IP ) 10 / CXC chemokine ligand 10 ( CXCL 10 ) and macrophage inflammatory protein 1beta / CC chemokine ligand 4 ( CCL 4 ) ] or Th 2 cells ( such as thymus and activation regulated chemokine / CCL17 and monocyte derived chemokine / CCL22 ) , respectively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : Skin biopsy specimens derived from pathognomonic lesions ( Gottron ' s papules and Gottron ' s sign ) of eight patients with DM were immunostained with a large panel of monoclonal antibodies to CD 3 , CD 4 , CD 8 , myeloperoxidase ( MPO ) , eosinophil cationic protein , tryptase , CD 40 , CD 40 ligand ( CD40L ) , HLA DR , interleukin ( IL ) 2 , IL 4 , IL 5 , IL 13 , interferon gamma , tumour necrosis factor alpha , receptor 3 for CXC chemokines ( CXCR 3 ) and receptor 3 for CC chemokines , using the alkaline phosphatase antialkaline phosphatase method . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These strategies include immunotherapy to directly enhance effector cell function of phagocytic cells ( e . g . , cytokine immunotherapy ) or skew immune responses toward protective type 1 immunity ( e . g . , CD 40 ligand , CpG dinucleotides , cytokines ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Special attention was paid to high sensitivity C reactive protein ( hs CRP ) and soluble CD 40 ligand ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We found that nafamostat mesilate elicits IL 12 , IL 18 , tumor necrosis factor alpha and interferon gamma production , and the expression of intercellular adhesion molecules 1 , B7 . 1 , B7 . 2 , CD 40 , and CD 40 ligand in human peripheral blood mononuclear cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : Hyper IgM 1 syndrome is a rare genetic primary immunodeficiency disease caused by mutations of the CD 40 ligand gene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This putative immunomodulatory role of basophils is supported further by their ability to express CD 40 ligand , which , together with IL 4 and IL 13 , serve as inductors of B cell proliferation and class switching to IgE and IgG 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Abnormalities in B lymphocyte CD 40 ligand ( CD 154 ) expression have been described for a number of immunologic diseases , including B cell lymphomas . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Skin biopsy showed perivascular eosinophilic infiltration in the dermis and CD 40 ligand ( CD40L ) positive eosinophilic infiltration . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have analyzed EBV infection in nine cases of 10 linked hyper immunoglobulin M ( hyper IgM ) syndrome who , due to a mutation in CD 40 ligand gene , do not have a classical , class switched memory B cell population ( IgD ( ) CD 27 ( + ) ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Associations between soluble CD 40 ligand , atherosclerosis risk factors , and subclinical atherosclerosis : results from the Dallas Heart Study . ^^^ OBJECTIVE : The purpose of this study was to evaluate the associations between plasma levels of soluble CD 40 ligand ( sCD40L ) , atherosclerosis risk factors , and evidence of subclinical atherosclerosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We observed that mast cells , as other cells expressing the CD 40 ligand CD 154 , can trigger IgE synthesis in B cells in the presence of interleukin ( IL ) 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Nevertheless , upon stimulation of lipopolysaccharide ( LPS ) or CD 40 ligand , the expression of CD 40 , CD 80 , CD 83 and CD 86 was upregulated on Ctrl DC but not on Dex DC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Previous studies have shown that iNKT produced IFNgamma and CD 40 ligand contribute to this dendritic cell maturation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These include tests for the presence of acute inflammation and infiltration ( e . g . , high sensitivity C reactive protein , myeloperoxidase ) , plaque instability ( e . g . , pregnancy associated plasma protein A , placental growth factor ) , platelet activation ( e . g . , whole blood choline , platelet density , CD 40 ligand ) , and myocardial ischemia ( e . g . , ischemia modified albumin , free fatty acids , serum choline , and B type natriuretic peptide ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interleukin 6 , soluble CD 40 ligand , and monocyte chemoattractant protein 1 levels decreased significantly ( by 20 to 50 % ) in both treatment groups on days 3 and 28 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Freshly isolated CCR 7 ( + ) early pre B ALL cells migrated to CCL 19 , whereas CCR 7 ( + ) pro B and pre B ALL cells were attracted by CCL 19 only following culture with soluble recombinant CD 40 ligand . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : We measured the concentration in blood of fibrinopeptide A , prothrombin fragment 1+2 , soluble CD 40 ligand , interleukin 1 receptor antagonist , interleukin 6 , and high sensitivity C reactive protein in 63 patients treated with aspirin and clopidogrel and undergoing elective percutaneous coronary intervention , who were randomized to treatment with either bivalirudin ( n=34 ) or unfractionated heparin plus eptifibatide ( n=29 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand ( CD40L ) is expressed by activated T cells and interacts with the CD 40 molecule present on B cells and endothelial cells , promoting immunoglobulin switching in the former and cytokine upregulation in the latter . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 8 T cells of OSA patients expressed a fourfold increase in TNF alpha and CD 40 ligand ( CD40L ) , and exhibited an increased OSA severity dependent cytotoxicity against endothelial cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Plasma TAFI and soluble CD 40 ligand do not predict reperfusion following thrombolysis for acute myocardial infarction . ^^^ Thrombin activatable fibrinolysis inhibitor ( TAFI ) and soluble CD 40 ligand ( sCD40L ) are novel endogenous fibrinolytic and atherothrombotic factors that determine clot stability . ^^^ We investigated whether admission plasma thrombin activatable fibrinolysis inhibitor ( TAFI ) and soluble CD 40 ligand ( sCD40L ) concentrations predicted reperfusion following thrombolytic therapy in patients with acute myocardial infarction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand influences platelet release of reactive oxygen intermediates . ^^^ OBJECTIVE : Soluble CD 40 ligand ( sCD40L ) has been recently implicated in the pathogenesis of atherosclerosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A recombinant human soluble CD 40 ligand had no effect on the proliferation of the resistant subclones . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Analysis of serum soluble CD 40 ligand in patients with influenza virus associated encephalopathy . ^^^ CD 40 ligand ( CD40L ) is mainly expressed on activated platelets and CD4+T cells , and it can be cleaved from the cell surface , releasing a soluble CD40L ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The relationship between soluble CD 40 ligand levels and Framingham coronary heart disease risk score in healthy volunteers . ^^^ Elevated soluble CD 40 ligand ( sCD40L ) levels are associated with an increased risk of cardiovascular events in patients with acute coronary syndromes and in middle aged healthy women . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Incubation with seed or skin extracts led to an immediate attenuation of release of the inflammatory mediator , soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Critical function of the CD 40 pathway in parvovirus B 19 infection revealed by a hypomorphic CD 40 ligand mutation . ^^^ This patient , whose serum IgG concentration was at the low end of the normal range and who mounted IgG antibody responses to T cell dependent antigens , suffered from a nonsense mutation ( R 11 > 10 ) in the CD 40 ligand ( CD40L ) gene . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Coexpression of CD 40 and CD 40 ligand in Epstein Barr virus infected T and NK cells and their role in cell survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To this end , we previously constructed an adenovirus with the fiber knob domain replaced by a fibritin trimerization motif fused to the CD 40 ligand ( Ad5Luc . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Levels of inflammatory markers / mediators such as CD 40 ligand ( CD40L ) and C reactive protein ( CRP ) were also measured in the patient plasmas utilizing ELISA tests . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Transfection of Trypanosoma cruzi with host CD 40 ligand results in improved control of parasite infection . ^^^ We have previously shown that infection by Trypanosoma cruzi , a parasitic protozoan , is reduced by injection of CD 40 ligand ( CD40L ) transfected 3T3 fibroblasts ( D . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We applied novel technologies , such as stimulation of CLL cells either with CD 40 ligand or with a combination of CpG oligodeoxynucleotides and IL 2 , to increase the frequency of metaphase spreads for detailed chromosome analysis in 96 patients with CLL . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Autoantibody to CD 40 ligand in systemic lupus erythematosus : association with thrombocytopenia but not thromboembolism . ^^^ OBJECTIVES : To examine the prevalence , clinical associations and pathogenic roles of autoantibodies to CD 40 ligand ( CD40L ) in patients with systemic lupus erythematosus ( SLE ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These biomarkers ( myeloperoxidase , metalloproteinase 9 , soluble CD 40 ligand , pregnancy associated plasma protein A , choline , ischemia modified albumin , unbound free fatty acids , glycogen phosphorylase isoenzyme BB , and placental growth factor ) have demonstrated promise and need to be more thoroughly evaluated for commercial development for implementation into routine clinical and laboratory practice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The positions of cysteine residues , CD 40 ligand binding amino acid residues , and four cysteine rich domains are well conserved in Japanese flounder CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Responses to human CD 40 ligand / human interleukin 2 autologous cell vaccine in patients with B cell chronic lymphocytic leukemia . ^^^ PURPOSE : Human CD 40 ligand activates the malignant B cell chronic lymphocytic leukemia cells and enhances their capacity to present tumor antigens . ^^^ Human interleukin 2 further potentiates the immunogenicity of human CD 40 ligand in preclinical murine models . ^^^ EXPERIMENTAL DESIGN : We prepared autologous B cell chronic lymphocytic leukemia cells that expressed both human CD 40 ligand ( > 90 % positive ) and human interleukin 2 ( median secretion , 1 , 822 pg / mL / 10 ( 6 ) cells ; range , 174 3 , 604 pg ) . ^^^ CONCLUSIONS : These results suggest that immune responses to B cell chronic lymphocytic leukemia can be obtained with human CD 40 ligand / human interleukin 2 expressing s . c . vaccines but that these responses are transient . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , protein expression of NIK is steadily induced by B cell activating factor or CD 40 ligand , two major physiological inducers of p 100 processing , but not by mitogen phorbol 12 myristate 13 acetate / ionomycin or cytokine tumor necrosis factor alpha , two well known inducers of the canonical NF kappaB signaling . ^^^ Remarkably , both B cell activating factor and CD 40 ligand do not significantly induce expression of NIK at translational or transcriptional level but rather rescue the basally translated NIK protein from undergoing degradation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : In 547 patients with angiographically validated ACS and in a heterogeneous emergency room population of 644 patients with acute chest pain , respectively , PAPP A as well as markers of myocardial necrosis ( troponin T [ TnT ] ) , ischemia ( vascular endothelial growth factor [ VEGF ] ) , inflammation ( high sensitivity C reactive protein [ hsCRP ] ) , anti inflammatory activity ( interleukin [ IL ] 10 ) , and platelet activation ( soluble CD 40 ligand [ sCD40L ] ) were determined . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Alloisotype switching coincided with T cell activation , as evidenced by increased serum levels of soluble CD 40 ligand costimulatory molecules . ^^^ Finally , a case control study showed that reduction of cardiac allograft rejection by mycophenolic acid was accompanied by reduced CD 40 ligand serum levels and the prevention of IgM to IgG anti HLA class 2 antibody switching . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Platelet aggregation , dense granule secretion , alpha granule secretion ( PAI 1 and soluble CD 40 ligand levels ) and procoagulant responses ( annexin 5 and microparticle formation ) were assessed using collagen and thrombin receptor activating peptide ( TRAP ) as agonists . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Compared with placebo , candesartan therapy significantly lowered plasma hsCRP levels relative to baseline measurements from 1 . 10 to 0 . 70 mg / l ( P=0 . 024 ) and soluble CD 40 ligand levels by 30+ / 11 % ( P < 0 . 001 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Proliferation was dependent on cell contact and on major histocompatibility complex ( MHC ) class 2 and CD 40 CD154 ( CD 40 ligand ) signaling . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Plasma , serum , and platelet expression of CD 40 ligand in adults with cardiovascular disease . ^^^ The application of soluble CD 40 ligand ( sCD40L ) as a biomarker has garnered great scientific and clinical interest . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of Src kinases combined with CD 40 ligand blockade prolongs murine cardiac allograft survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OCH induced lower IL 12 production due to ineffective primary IFN gamma and CD 40 ligand expression by NKT cells , and resulted in lower secondary IFN gamma induction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This activation could be blocked completely by antibodies directed against the CD 40 ligand ( CD 154 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , although MoDCs matured with TNF and PGE 2 selectively produced the inhibitory IL 12p40 subunit at steady state , they were able to produce the bioactive IL 12p70 heterodimer after stimulation with CD 40 ligand and / or IFN gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we show that plasmid DNA vaccination with a cassette encoding antigen ( OVA ) and a second cassette encoding full length CD 40 ligand ( CD40L ) , a molecule expressed on activated CD4+ T lymphocytes and critical for T cell helper function , can elicit significant titers of antigen specific immunoglobulins in serum and Tc 1 CD8+ T cell responses in CD 4 deficient mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we report the second case of a female HIGM 1 with the defect of CD 40 ligand ( CD40L ) expression and of soluble serum CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blood was taken prior to PCI for baseline measurement of : ( 1 ) vasoconstrictive factors : endothelin ( ET ) and serotonin ( 5 HT ) ; ( 2 ) thrombotic factors : tissue factor ( TF ) , plasminogen activator inhibitor ( PAI 1 ) , thrombin / antithrombin 3 complex ( TAT ) , and prothrombin fragment F1+2 ( F1+2 ) ; and ( 3 ) inflammatory factors : soluble CD 40 ligand ( sCD40L ) and soluble E selectin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inverse correlation between soluble CD 40 ligand and soluble CD 40 is absent in patients with unstable angina . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This study investigated the expression of CD 40 , CD 40 ligand ( CD40L ) and matrix metalloproteinases ( MMPs ) in dietary induced atherosclerosis in rats . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti inflammatory effects of clopidogrel intake in renal transplant patients : effects on platelet leukocyte interactions , platelet CD 40 ligand expression , and proinflammatory biomarkers . ^^^ Degranulation ( CD62P ) , glycoprotein IIb / IIIa receptor activation ( PAC 1 ) , formation of platelet leukocyte aggregates ( monocyte platelet leukocyte aggregate , CD11b , mean fluorescence intensity ) , and platelet CD 40 ligand ( CD40L ) expression ( percent positive ) were assessed by flow cytometry before therapy ( visit 1 ) and after 4 weeks of clopidogrel ( 75 mg / d ) intake ( visit 2 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Priming target leukemic cells with CD 40 ligand and IL 4 enhanced CTL killing . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Abnormal soluble CD 40 ligand and C reactive protein concentrations in hypertension : relationship to indices of angiogenesis . ^^^ OBJECTIVE : To test the hypothesis that concentrations of high sensitive C reactive protein ( CRP , an index of inflammation ) and soluble CD 40 ligand ( sCD40L , an index of platelet activation ) would be abnormal in hypertension , and in turn , be related to plasma indices of angiogenesis , the angiopoietins 1 and 2 , and vascular endothelial growth factor ( VEGF ) , in addition to the presence or absence of CVD . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have previously reported that exposure to CD 40 ligand and IL 4 could induce LMP 1 in an in vitro EBV infected Hodgkin lymphoma derived cell line , which expressed only EBNA 1 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Flavonoids including luteolin , apigenin , and fisetin are inhibitors of IL 4 synthesis and CD 40 ligand expression by basophils . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The second is a two event model : the first event is the clinical condition of the patient resulting in pulmonary endothelial activation and PMN sequestration , and the second event is the transfusion of a biologic response modifier ( including anti granulocyte antibodies , lipids , and CD 40 ligand ) that activates these adherent PMNs resulting in endothelial damage , capillary leak , and TRALI . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD4+ T lymphocytes expressing CD 40 ligand help the IgM antibody response to soluble pneumococcal polysaccharides via an intermediate cell type . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of atorvastatin on circulating proinflammatory T lymphocyte subsets and soluble CD 40 ligand in patients with stable coronary artery disease a randomized , placebo controlled study . ^^^ METHODS : To investigate the effect of atorvastatin on circulating T lymphocyte subsets producing proinflammatory and anti inflammatory cytokines ( interferon gamma [ IFN gamma ( + ) ] , interleukin 2 [ IL 2 ( + ) ] , IL 4 ( + ) , and IL 10 ( + ) ) and on the T cell activating soluble CD 40 ligand ( sCD40L ) , 30 hypercholesterolemic patients with angiographically documented stable coronary artery disease ( CAD ) were randomized to placebo or atorvastatin ( 20 mg / d ) for 3 months . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Proliferation of Cd 45 / B cells induced by CD 40 ligand in vitro was impaired as a result of abrogation by BCR ligation of the upregulation of prosurvival proteins . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Costimulation blockade of both inducible costimulator and CD 40 ligand induces dominant tolerance to islet allografts and prevents spontaneous autoimmune diabetes in the NOD mouse . ^^^ Using a fully allogeneic mouse model , we tested the effectiveness of the combined blockade of the CD 40 ligand and the inducible costimulator ( ICOS ) on islet allograft survival and in the prevention of autoimmune diabetes in the NOD mouse . ^^^ Recipients treated with blocking monoclonal antibodies ( mAbs ) to ICOS and the CD 40 ligand had significant prolongation of graft survival , with 26 of 28 functioning for > 200 days . ^^^ Combined anti ICOS and anti CD 40 ligand mAb therapy also prevented diabetes in NOD mice , with only 11 % of treated recipients developing diabetes compared with 75 % of controls . ^^^ These data demonstrate that the blockade of CD 40 ligand and ICOS signaling induces islet allograft tolerance involving a dominant mechanism associated with intragraft regulatory cells and prevents autoimmune diabetes in NOD mice . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effects of hyperglycemia and hyperinsulinemia on circulating tissue factor procoagulant activity and platelet CD 40 ligand . ^^^ Combined elevations of plasma insulin and glucose levels for 24 h produced a ninefold increase in tissue factor PCA , which was associated with an increase in monocyte tissue factor protein ( flow cytometry ) and mRNA ( RT PCR ) , increases in plasma thrombin antithrombin complexes , prothrombin fragment 1 . 2 , factor 8 coagulant activity , and platelet CD 40 ligand as well as decreases in factor VIIa , factor 7 coagulant activities , and factor 7 antigen . ^^^ We conclude that hyperinsulinemia and hyperglycemia but particularly the combination of both create a prothrombotic state and in addition may be proinflammatory and proatherogenic because of the proinflammatory actions of CD 40 ligand and tissue factor . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
One of the major defects in HIV appears to be the failure of CD 4 T cells to provide CD 154 ( CD 40 ligand ) mediated help , which is required for APC function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation of B CLL cells through CD 40 ligand ( CD40L ) stimulation decreases expression of the mHAg encoding PANE 1 transcript and reciprocally increases expression of PANE 1 transcripts lacking the mHAg encoding exon . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 154 ( CD 40 ligand ) expression on CD 4 T cells is normally tightly controlled , but abnormal or dysregulated expression of CD 154 has been well documented in autoimmune diseases , such as systemic lupus erythematosus . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand , urinary 11 dehydro thromboxane B 2 , serum thromboxane B 2 , soluble P selectin , and von Willebrand factor were measured as biomarkers of in vivo platelet activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : In 544 patients who were enrolled in the placebo arm of the c7E3 Fab Anti Platelet Therapy in Unstable REfractory angina ( CAPTURE ) trial , PlGF levels were determined as well as markers of myocardial necrosis ( troponin T [ TnT ] ) , general inflammation ( high sensitivity C reactive protein [ hsCRP ] ) , and platelet activation ( soluble CD 40 ligand [ sCD40L ] ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using mouse polyoma virus ( PyV ) as a model of low level persistent virus infection , we asked whether blockade of the CD 40 ligand ( CD40L ) and CD 28 costimulatory pathways impacts the magnitude and function of the PyV specific CD8+ T response , as well as the humoral response and viral control during acute and persistent phases of infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we demonstrate that membrane presented CD 40 ligand ( CD 154 ) ( mCD40L ) , but not soluble agonists , triggers cell death in malignant human urothelial cells via a direct mechanism involving rapid upregulation of TNFR associated factor ( TRAF ) 3 protein , without concomitant upregulation of TRAF 3 mRNA , followed by activation of the c Jun N terminal kinase ( JNK ) / activator protein 1 ( AP 1 ) pathway and induction of the caspase 9 / caspase 3 associated intrinsic apoptotic machinery . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Multimeric soluble CD 40 ligand and GITR ligand as adjuvants for human immunodeficiency virus DNA vaccines . ^^^ CD 40 ligand ( CD40L ) , a member of the tumor necrosis factor ( TNF ) superfamily ( TNFSF ) , is one candidate adjuvant , but it has been difficult to use because it is normally expressed as a trimeric membrane molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Impact of clopidogrel on suppression of circulating levels of soluble CD 40 ligand in patients with unstable angina undergoing coronary stenting . ^^^ This study investigated whether a regimen that comprised a loading dose of 300 mg of clopidogrel followed by 75 mg / day could significantly suppress circulating levels of soluble CD 40 ligand ( sCD40L ) in patients who had unstable angina and underwent coronary stenting . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Usefulness of preprocedural soluble CD 40 ligand for predicting restenosis after percutaneous coronary intervention in patients with stable coronary artery disease . ^^^ We investigated the effect of preprocedural CD 40 ligand ( sCD40L ) on stent restenosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We performed a randomized controlled trial to assess whether exercise training decreases levels of 5 inflammatory markers linked to future cardiovascular risk : white blood cell count , fibrinogen , C reactive protein , soluble intercellular adhesion molecule 1 , and soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thromboxane dependent CD 40 ligand release in type 2 diabetes mellitus . ^^^ OBJECTIVES : The goals of this study were to characterize the platelet contribution to soluble CD 40 ligand ( sCD40L ) , to correlate its formation with the extent of oxidative stress and platelet activation , and to investigate the effects of improved metabolic control and low dose aspirin on these processes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
They also produce interferon gamma and CD 40 ligand to stimulate surrounding cells to produce matrix metalloproteases and cysteine proteases for aortic matrix remodeling . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , ex vivo conditioning of DCs with CD 40 ligand and IL 12 was protective against tumor induced apoptosis . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Taking advantage of the HSVQuik system , we constructed three oncolytic HSV vectors that express mouse IL 4 , CD 40 ligand and 6CK , respectively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased CD 40 ligand expression on platelets is associated with cardiovascular events after total hip arthroplasty . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
PTX 3 production was induced by TLR ligands , CD 40 ligand , and interleukin ( IL ) 1beta and was suppressed by dexamethasone , 1alpha , 25 dihydroxivitamin D 3 , and prostaglandin E 2 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Consistent with an immature status , their lack of proliferation to B cell receptor triggering was reversed with CD 40 ligand , but not B cell activation factor . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The capacity of DC stimulation to prevent apoptosis was assessed by incubating DC with inflammatory cytokines , poly 1 : C , IL 12 or CD 40 ligand ( CD40L ) prior to culture with TDSN . ^^^ Aiming to identify factors that protect blood DC from apoptosis , we compared a range of clinically available maturation stimuli , including inflammatory cytokines ( tumor necrosis factor alpha , IL 1beta , IL 6 and prostaglandin ( PG ) E 2 as a cytokine cocktail ) , synthetic double stranded RNA ( poly 1 : C ) and soluble CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Chronic activation of skin dendritic cells by keratinocyte expression of CD 40 ligand ( CD40L ; CD 154 ) leads to autoimmunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , stimulation of B CLL cells with CD 40 ligand plus TNFalpha increased Cox 2 levels . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effects of clopidogrel on soluble CD 40 ligand and on high sensitivity C reactive protein in patients with stable coronary artery disease . ^^^ Our study was designed to test the effects of clopidogrel on soluble CD 40 ligand ( sCD40l ) and on high sensitivity C reactive protein ( hs CRP ) in patients with stable coronary artery disease ( CAD ) . ^^^ Soluble CD 40 ligand and hs CRP were measured at baseline and at completion of the study . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
C 274 induced macaque CD 20 ( + ) B cells to proliferate more strongly than CD 40 ligand or CpG B ISS ODN . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When Tu 183 cells were exposed to the CD 40 ligand they showed apoptosis , while NHEK cells did not . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interleukin ( IL ) 18 , which is elevated in the plasma during acute rejection after organ transplantation , is known to induce the expression of intercellular adhesion molecule ( ICAM ) 1 , B7 . 1 , B7 . 2 , CD 40 and CD 40 ligand ( CD40L ) on monocytes , the production of interferon ( IFN ) gamma and IL 12 and the proliferation of lymphocytes during the human mixed lymphocyte reaction ( MLR ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunoglobulin class switching from immunoglobulin M ( IgM ) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD 154 ( CD 40 ligand ) and cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Participation of complement activation , and CD 40 , CD 40 ligand interactions have been demonstrated in AT and SLE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : The CD 40 negative oral keratinocyte line OSC 19 , its CD 40 positive transfected derivative ( OSC19T CD 40 ) and null transfectants ( OSC19T control ) , with and without stimulation by soluble protein CD 40 ligand ( sCD40L ) or anti CD 40 antibodies were used . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Platelet count , soluble CD 40 ligand , fibrinogen , antithrombin , D Dimer , prothrombin fragment 1 . 2 ( F1 . 2 ) , and free plasma hemoglobin levels were measured at baseline , at aortic unclamping , and at arrival in the intensive care unit . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Enhancement of binding activity of soluble human CD 40 to CD 40 ligand through incorporation of an isoleucine zipper motif . ^^^ AIM : To investigate the effect of incorporation of an isoleucine zipper ( IZ ) motif into CD 40 on binding activity of CD 40 for the CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inflammatory biomarkers , including high sensitivity C reactive protein ( hs CRP ) , matrix metalloproteinase 9 ( MMP 9 ) , soluble CD 40 ligand ( sCD40L ) , and adiponectin , and lipid profiles were measured at the time of initiation , after rosiglitazone monotherapy and after combination therapy with rosiglitazone and atorvastatin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Long term influence of regular intake of high dose n 3 fatty acids on CD 40 ligand , pregnancy associated plasma protein A and matrix metalloproteinase 9 following acute myocardial infarction . ^^^ The adhesion molecule CD 40 ligand ( CD40L ) , expressed on activated platelets and T lymphocytes , can activate metalloproteinases and thereby promote plaque rupture . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and risk of ischemic stroke or coronary events in patients with chronic coronary heart disease . ^^^ BACKGROUND : CD 40 ligand ( CD40L ) is a trimeric , transmembrane protein of the tumor necrosis factor family and together with its receptor CD 40 is an important contributor to the inflammatory processes that lead to atherosclerosis , plaque destabilization , and thrombosis . ^^^ METHODS : In order to assess the association between serum concentrations of CD 40 ligand ( CD40L ) and risk of future ischemic stroke and coronary events among patients with chronic CHD , we obtained baseline serum samples from patients ( n = 3090 ) with chronic CHD enrolled in a secondary prevention trial . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Fasting blood was obtained for biomarkers of inflammation ( C reactive protein [ CRP ] , plasma soluble cell adhesion molecules [ CAMs ] , monocyte chemoattractant protein 1 , nitrotyrosine , CD 40 ligand [ CD40L ] , and monocyte function ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Therefore , we sought to investigate the degree and character of inflammation in children with heterozygous familial hypercholesterolaemia ( FH ) by measuring levels of neopterin , high sensitivity C reactive protein ( hsCRP ) , and soluble CD 40 ligand ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : 10 linked hyper immunoglobulin M ( IgM ) syndrome ( XHIGM ) is a rare immunodeficiency disease caused by mutations of the CD 40 ligand gene . ^^^ METHODS : Of 37 living family members , mutational analysis of the CD 40 ligand gene was performed in 36 members . ^^^ The 3 patients with XHIGM syndrome and the 5 carriers all had a novel mutation located at Tyr 169 Asn ( T526A ) in exon 5 , the tumor necrosis factor domain of the CD 40 ligand gene . ^^^ In the 3 patients , CD 40 ligand expression in activated CD4+ T cells was below 1 % . ^^^ In the carriers , about half of activated CD4+ cells expressed CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Similar to the situation in normal B cells , in vitro stimulation of MCL cell lines with CD 40 ligand ( CD40L ) and interleukin 4 induced expression of activation induced cytidine deaminase ( AID ) and germline transcription at the immunoglobulin heavy chain gene locus . ^^^ Our data indicate that mantle cell lymphomas have retained the ability to undergo class switch recombination if appropriate stimuli , such as the CD 40 ligand , are provided . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Classification of mutations in the human CD 40 ligand , gp 39 , that are associated with 10 linked hyper IgM syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand in autoimmune diseases and atherosclerosis ] . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The key role of CD 40 ligand in overcoming tumor induced dendritic cell dysfunction . ^^^ CD 40 ligand ( CD40L ) , a key molecule in the interface between T lymphocytes and DCs , seems to be instrumental in achieving that goal . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Influence of sample type and storage conditions on soluble CD 40 ligand assessment . ^^^ BACKGROUND : Several studies have consistently shown that soluble CD 40 ligand ( sCD40L ) concentrations are increased in patients with acute coronary syndromes and can serve as a biomarker for risk stratification . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
An important cognate event in the development of cell mediated immunity is the interaction between CD 40 and CD 40 ligand . ^^^ In addition to CD 40 ligand ( expressed by activated Th cells ) , inflammatory cytokines , bacterial components or Ag Ab immune complexes can induce maturation of DC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Effect of continuous positive airway pressure on soluble CD 40 ligand in patients with obstructive sleep apnea syndrome . ^^^ We measured serum levels of soluble CD 40 ligand ( sCD40L ) , tumor necrosis factor ( TNF ) alpha , and hypersensitive C reactive protein ( hsCRP ) before and after nasal continuous positive airway pressure ( nCPAP ) therapy for 3 months . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand in prediction of acute severe pancreatitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand and anti CD 40 antibodies have been used successfully to induce apoptosis in lymphoma lines both in vitro and in xenograft tumor models . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , diabetes was prevented when DNA vaccination was performed under the cover of CD 40 ligand blockade , known to prevent priming of CTL by mucosal Ag . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Luteolin , a flavonoid , inhibits CD 40 ligand expression by activated human basophils . ^^^ In this study , we investigated whether luteolin can suppress CD 40 ligand expression by basophils . ^^^ METHODS : A human basophilic cell line , KU 812 , was stimulated with A 23187 and phorbol myristate acetate ( PMA ) with or without various concentrations of luteolin or other flavonoids for 12 h , and CD 40 ligand expression was analyzed by FACS . ^^^ The effect of luteolin on CD 40 ligand mRNA expression was studied by semiquantitative reverse transcription PCR analysis . ^^^ In addition , CD 40 ligand expression was also measured in purified basophils that had been stimulated for 12 h with A 23187 plus PMA with or without various concentrations of luteolin . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To evaluate whether the interleukin 6 ( IL 6 ) 174 G / C polymorphism might alter the effects of micronized fenofibrate or simvastatin therapy on inflammatory markers , we measured IL 6 , C reactive protein , CD 40 ligand , adhesion molecules , P selectin and monocyte chemoattractant protein 1 in hypercholesterolemic patients both before and after a 30 day treatment . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this article , we review clinical importance of novel biomarkers referring our previous clinical investigation and other reports , especially troponin T for detection of minor myocardial damage associated with vulnerable plaque with thrombus / embolus , heart type fatty acid binding protein for earlier detection of myocardial damage and it ' s role for the rule out triage , N terminal pro BNP for earlier risk stratification in cardiac emergency , and soluble CD 40 ligand for earlier identification of plaque destabilization with platelet activation in non ST elevation acute coronary syndrome . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Serum soluble CD 40 ligand in normal pregnancy and in preeclampsia . ^^^ OBJECTIVE : Soluble CD 40 ligand is a transmembrane protein shed from activated platelets that is involved in the activation of endothelial cells . ^^^ Findings that estradiol ( E 2 ) has an inhibitory effect on inflammation and platelet function and that serum E 2 levels are low in women with preeclampsia prompted us to investigate the association between soluble CD 40 ligand and serum E 2 levels in normal pregnancy and in preeclampsia . ^^^ Enzyme immunoassay was used to measure soluble CD 40 ligand . ^^^ RESULTS : Significantly higher levels of soluble CD 40 ligand were detected in the nonpregnant women ( 23 , 767 + / 15 , 637 pg / mL ) and in the women with preeclampsia ( 21 , 025 + / 45 , 386 pg / mL ) than in the normotensive pregnant women ( 8 , 292 + / 5 , 926 pg / mL ) ( P = . 026 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated circulating CD 40 ligand in patients with erythema multiforme and Stevens Johnson syndrome / toxic epidermal necrolysis spectrum . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand and platelet derived microparticles significantly increased after both auto and allo SCT . ^^^ The increase of RANTES seems to be related to platelet activation , since RANTES was in the dynamic phase similar to soluble CD 40 ligand and platelet derived microparticles . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Levels of serum sVCAM 1 ( 614 vs 494 ng / mL , P = 0 . 002 ) , oxidized LDL ( 144 vs 97 , P < 0 . 001 ) and CD 40 ligand ( 4385 vs 1373 pg / ml , P = 0 . 001 ) were significantly higher in SLE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Diverse associations of microalbuminuria with C reactive protein , interleukin 18 and soluble CD 40 ligand in male essential hypertensive subjects . ^^^ We investigated whether urinary albumin excretion , expressed as the albumin to creatinine ratio ( ACR ) , is associated with high sensitivity C reactive protein ( hs CRP ) , interleukin ( IL ) 18 , and soluble CD 40 ligand ( sCD40L ) , in hypertensive subjects . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Tumor necrosis factor alpha ( TNFalpha ) , interleukin 6 ( IL 6 ) , monocyte chemoattractant protein 1 ( MCP 1 ) and CD 40 ligand ( CD40L ) were all significantly correlated with the angiographic score ( p < 0 . 05 for all ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These new treatments , which target various steps of the immune response , include : B lymphocyte ( BL ) inhibitors , such as anti CD 20 monoclonal antibodies , BlyS antagonists , and tolerogens that inhibit specific BLs that produce pathogenic antibodies ; inhibitors of costimulation between antigen presenting cells and T lymphocytes ( TL ) , such as monoclonal antibodies against CD 40 ligand and CTLA 4 Ig ( abatecept ) ; TL antagonists , which can inhibit proliferation of autoreactive T cells ; cytokine antagonists ; chemokine and adhesin antagonists , which inhibit trafficking of immunocompetent cells to target organs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Elevated levels of soluble CD 40 ligand ( sCD40L ) in serum of patients with systemic autoimmune diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Serum concentrations of adiponectin , C reactive protein , N terminal pro brain natriuretic peptide ( NT proBNP ) , interleukin ( IL ) 1beta , IL 6 , IL 8 , IL 10 , IL 12 , tumour necrosis factor alpha and CD 40 ligand were determined . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This enhancement did not depend on interferon gamma ( IFN gamma ) , TNF alpha , or CD 40 ligand but did depend on cell to cell contact . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : Activation of nave B lymphocytes by extracellular ligands , e . g . antigen , lipopolysaccharide ( LPS ) and CD 40 ligand , induces a combination of common and ligand specific phenotypic changes through complex signal transduction pathways . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we describe a novel type of organized lymphoid structure in the lung of human idiopathic pulmonary fibrosis , with key features of lymphoid neogenesis , including : 1 ) recently activated CD 40 ligand ( CD40L ) + T cells ; 2 ) variable numbers of activated CD40+ / CD40L+ B cells , sometimes organized in follicles ; 3 ) fully mature dendritic cells ( DC ) expressing CD 40 , CD 83 , CD 86 , and DC lysosome associated membrane protein ; 4 ) the expression of the chemokine CCL 21 ; 5 ) the presence of vessels with characteristics of high endothelial venules ; and 6 ) a dense network of follicular DC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
During cognate interaction with CD 40 ligand ( CD 154 ) expressing T cells , Ag presenting accessory cells are activated for increased cytokine synthetic and costimulatory function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 ligand ( CD40L ) CD 40 dyad can ignite proinflammatory and procoagulatory activities of the vascular endothelium in the pathogenesis and progression of atherosclerosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent studies suggest that soluble CD 40 Ligand ( sCD40L ) may play a pathogenetic role in atherothrombotic complications in cardiovascular disease as well as in inflammation and thrombosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We developed a multiplexed immunoassay to simultaneously assess the plasma concentrations of 8 cytokines ( interferon gamma , interleukin ( IL ) 2 , IL 4 , IL 6 , IL 8 , IL 10 , IL 12 , and tumor necrosis factor alpha ) , IL 2r , and soluble CD 40 ligand in the patient groups . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVES : The aim of this research was to evaluate the effect of genetic polymorphisms C807T and G1648A of platelet glycoprotein Ia ( GPIa ) , on the risk for myocardial infarction ( MI ) and on the release of soluble CD 40 ligand ( sCD40L ) during the acute phase of MI and one year after the event . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : To detect the levels of index related to inflammation such as soluble CD 40 ligand ( sCD40L ) , neutrophil collagenase 8 ( MMP 8 ) , and pregnancy associated plasma protein A ( PAPP A ) , lipid peroxidation and autoimmune indexes such as oxidized low density lipoprotein ( ox LDL ) and its antibody ( ox LDL Ab ) in patients with coronary heart disease , and to investigate its relationship with acute coronary syndrome ( ACS ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , differences between CD 80 and CD 86 costimulation extended to the T cell phenotype , in particular the levels of CD 40 ligand expression . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Increased levels of the CD 40 : CD 40 ligand dyad in the cerebrospinal fluid of rats with vitamin B 12 ( cobalamin ) deficient central neuropathy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : CD 40 of RASFs was ligated with CD 40 ligand ( CD40L ) transfected L cells or activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : Immune responses require costimulatory interactions between molecules on antigen presenting cells and T cells : CD 40 binding to CD 40 ligand and B 7 binding to CD 28 . ^^^ We attempted to modulate Ad A subunit induced Graves ' disease using adenoviruses expressing costimulation `` decoys ' ' : CD 40 IgG Fc ( CD 40 Ig ) to block CD 40 : CD 40 ligand interactions and CTLA 4 Fc ( CTLA 4 Ig ) to prevent B 7 : CD 28 binding . ^^^ CONCLUSIONS : Using appropriate controls and adenovirus ratios , our data suggest the importance of CD 40 : CD 40 ligand interactions for inducing Graves ' hyperthyroidism by Ad A subunit . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Additional activation with CD 40 ligand and interferon ( IFN ) gamma increased viability of DC and promoted definitive maturation as defined by maintenance of a mature phenotype after withdrawal of cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand accumulates in stored blood components , primes neutrophils through CD 40 , and is a potential cofactor in the development of transfusion related acute lung injury . ^^^ Soluble CD 40 ligand ( sCD40L ) is a platelet derived proinflammatory mediator that accumulates during platelet storage . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The generation of thymus independent germinal centers depends on CD 40 but not on CD 154 , the T cell derived CD 40 ligand . ^^^ In this report , we show that the formation of germinal center ( GC ) like structures to thymus independent type 2 antigens in mice depends on intact signals through CD 40 , but does not depend on T cell derived CD 40 ligand ( CD 154 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Overall , responses of neonatal CD 4 ( + ) T cells to PTX were similar to those of adult CD45RA ( + ) CD 4 ( + ) naive T cells except for their blunted CD 40 ligand up regulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interaction between CD 40 on cDCs and CD 40 ligand on pDCs led to IL 12 production by cDCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Serum levels of platelet released CD 40 ligand are increased in early onset occlusive carotid artery disease . ^^^ OBJECTIVE : Soluble CD 40 ligand ( sCD40L ) has been suggested as a key mediator between inflammation and atherosclerosis , and the CD 40 CD40L interaction has a role in atherosclerotic lesion progression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVES : To assess the diagnostic value of a modified serum induced basophil activation test , the CD 63 expression assay , in the diagnosis of ACU by comparing the results of the CD 63 assay with the results of the histamine release ( HR ) test , the ASST and serum levels of soluble CD 40 ligand ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Biologically active CD 40 ligand is elevated in sickle cell anemia : potential role for platelet mediated inflammation . ^^^ OBJECTIVE : After activation , platelets expose CD 40 ligand ( CD40L ) on their surface , then subsequently release the inflammatory mediator as a soluble fragment ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The presence of metabolic syndrome is independently associated with elevated serum CD 40 ligand and disease severity in patients with symptomatic coronary artery disease . ^^^ However , to date , no studies have investigated the effect of metabolic syndrome on circulating soluble CD 40 ligand ( sCD40L ) , monocyte chemoattractant protein 1 , cellular adhesion molecules , and disease severity in patients with symptomatic coronary artery diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Randomized comparison of the effects of ASA plus clopidogrel versus ASA alone on early platelet activation in acute coronary syndromes with elevated high sensitivity C reactive protein and soluble CD 40 ligand levels . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Platelet induced modulation of inflammation seems to involve platelet expression of ligands in the tumor necrosis factor ( TNF ) superfamily such as CD 40 ligand and Fas ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Induction of murine liver damage by overexpression of CD 40 ligand provides an experimental model to study fulminant hepatic failure . ^^^ Previously , we demonstrated that intrahepatic upregulation of the immunoactivating molecules CD 40 and CD 40 ligand ( CD40L ) are early mechanisms for liver cell damage in human and murine fulminant hepatic failure ( FHF ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Soluble CD 40 ligand in systemic lupus erythematosus and antiphospholipid syndrome ] . ^^^ AIM : To investigate a clinical role of soluble ( s ) CD 40 ligand in systemic lupus erythematosus ( SLE ) and antiphospholipid syndrome ( APS ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Lipids , high sensitive CRP , soluble CD 40 ligand ( CD40L ) and von Willebrand factor ( vWF ) activity were assessed on days 1 ( baseline ) , 4 , 7 , 14 , 30 and ( except CD40L ) 90 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IFN gamma production was correlated with CD 40 ligand expression on the tumor and inversely with interleukin 10 ( IL 10 ) production by B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Coronary artery disease progression is associated with C reactive protein and conventional risk factors but not soluble CD 40 ligand . ^^^ Epidemiological studies have documented conventional risk factors ; however , no studies to date have addressed the roles of soluble CD 40 ligand ( sCD40L ) and monocyte chemoattractant protein 1 ( MCP 1 ) , and there have been few reports on other novel risk factors in CAD progression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interleukin ( IL ) 6 , IL 8 , PLT derived growth factor ( PDGF ) AA , soluble CD 40 ligand ( sCD40L ) , RANTES , and transforming growth factor beta production were measured by specific enzyme linked immunosorbent assays . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we show that a subset of B cells in the blood and tonsils of normal donors expressed DC SIGN , and that this increased after stimulation in vitro with interleukin 4 and CD 40 ligand , with enhanced expression of activation and co stimulatory molecules CD 23 , CD 58 , CD 80 , and CD 86 , and CD 22 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand is an important immunoregulatory protein expressed by T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blood dendritic cells generated with Flt 3 ligand and CD 40 ligand prime CD8+ T cells efficiently in cancer patients . ^^^ These immature DCs can be rapidly activated by soluble CD 40 ligand ( CD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
DCs were generated by culturing monocytes with granulocyte macrophage colony stimulating factor ( granulocyte macrophage colony stimulating factor ) and interleukin ( IL 4 ) and activated by additional culture with tumor necrosis factor and CD 40 ligand . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
An association of soluble CD 40 ligand ( CD 154 ) with adverse reactions to platelet transfusions . ^^^ PLTs are the primary source of soluble CD 40 ligand ( sCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The functional role of CD 40 mediated interactions in modifying immune responses to Y 79 was assessed in vitro by using recombinant human CD 40 ligand ( rhCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The authors investigated 232 consecutive patients with CLI by means of electrocardiogram ( ECG ) , and measurements of endothelin ( ET ) 1 , tumor necrosis factor alpha ( TNF ) alpha , interleukin ( IL ) 6 , neopterin , CD 40 ligand , and 8 epi prostaglandin ( PG ) F2alpha in plasma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Alpha linolenic acid rich wheat germ oil decreases oxidative stress and CD 40 ligand in patients with mild hypercholesterolemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligand increases expression of its receptor CD 40 in human coronary artery endothelial cells . ^^^ BACKGROUND : Recently , CD 40 ligand ( CD40L ) and its receptor CD 40 have been implicated in atherosclerosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Serial biomarkers C reactive protein , troponin , creatine kinase MB , soluble CD 40 ligand , interleukin 6 , prothrombin fragment F1 . 2 , and RANTES ( regulated on activation , normal T cell expressed and secreted ) were assessed through 24 hours after PCI . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The human T cell antigen gp 39 , a member of the TNF gene family , is a ligand for the CD 40 receptor : expression of a soluble form of gp 39 with B cell co stimulatory activity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interactions between gp 39 ( CD40L , TRAP , T BAM ) on activated T cells and CD 40 on antigen presenting cells play an important role in regulating antibody production by B cells , cytokine production by monocytes , and other immune responses which require T cell `` help ' ' . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction between gp 39 ( CD40L , TRAP , T BAM ) on activated T cells and mast cells and CD 40 on antigen presenting cells modulates immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We used a murine syngeneic bone marrow transplant ( BMT ) model , in which administration of anti CD 40 antibody early after BMT results in overproduction of interleukin 12 ( IL 12 ) and interferon gamma ( IFN gamma ) , and lethal gut toxicity to examine the protective effect of the spin trap inhibitor , alpha phenyl tert butyl nitrone ( PBN ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rescue from anti IgM induced programmed cell death by the B cell surface proteins CD 20 and CD 40 . ^^^ To explore the effect of physiologic signals on anti IgM induced PCD , antibodies against the CD 20 or CD 40 molecules were added in concert with anti IgM . ^^^ Both CD 20 and CD 40 synergize with anti IgM to augment proliferation but neither molecule activates PKC in Ramos cells . ^^^ Both anti CD 20 and anti CD 40 reduced the number of cells undergoing anti IgM induced PCD . ^^^ Unlike the effect of anti CD 40 , addition of anti CD 20 to anti IgM stimulated cells negated PCD only in a subset of cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IgM and IgG constituted the predominant Ig isotype produced by sIgD+ and sIgD B cells , respectively , in response to IL 10 . sIgD+ B cells could be induced for IgA synthesis upon co culturing with transforming growth factor beta ( TGF beta ) and IL 10 , in the presence of an anti CD 40 monoclonal antibody presented by the CDw 32 L cells . ^^^ In contrast , TGF beta suppressed the IL 10 mediated IgG , IgM , and IgA secretions by sIgD B cells . sIgD+ B cells could not be induced for IgA synthesis by TGF beta and IL 10 after crosslinking of their sIgs , suggesting that ligation of CD 40 was one of the obligatory signals required for commitment of naive B cells to IgA secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD5+ B cells could be stimulated for DNA synthesis by mitogenic concentrations of Staphylococcus aureus , Cowan 1 strain ( SAC ) , insolubilized anti IgM antibodies , immobilized anti CD 40 antibodies and phorbol 12 myristate 13 acetate ( PMA ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The effect of the combination of anti CD 40 and IL 4 on IgE production was very IgE isotype specific as IgG , IgM , and IgA were not increased . ^^^ Furthermore , anti CD 40 with IL 5 or PWM did not co stimulate IgG , IgM , or IgA and in fact strongly inhibited PWM stimulated IgG , IgM and IgA production from blood or tonsil cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Next , we identified three signals , each of which was capable of selectively counteracting the inhibitors of IL 4 promoted CD 23 production : ( a ) the engagement of surface CD 40 antigen with mAb was found to override the influence of all the inhibitors of CD 23 expression ; ( b ) mAb to surface IgM overcame the inhibitory actions of TGF beta and interferons but not that of CD 19 ligation ; ( c ) ligation of surface CD 72 counteracted the inhibition mediated by TGF beta but not that generated by interferons or anti CD 19 antibody . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Triggering of neoplastic B cells via surface IgM and the cell surface antigens CD 20 and CDw 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Regulation of resting and cycling human B lymphocytes via surface IgM and the accessory molecules interleukin 4 , CD 23 and CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Hypercross linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL 4 and anti CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The latter observation suggests that there is a T dependent , CD 40 ligand independent pathway of B cell activation that leads to IgM responses and that a significant component of the IgM in HIMG 1 patients is derived from T dependent responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Additionally , B lymphocytes cultured in the CD 40 system in the presence of human IL 10 , produce IgM , IgG , and IgA , and Ig levels are further increased by SAC . ^^^ Thus , SAC and anti CD 40 mAb stimulate B cells to differentiate into cells secreting IgG and IgM whereas IL 10 plays a central role in inducing B cells from IgA D patients to differentiate into IgA secreting cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , IL 2 significantly augmented the synthesis of IgM , IgA , and IgG , including all IgG subclasses by anti CD 40 activated B cells cultured in the presence of IL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Involvement of LFA 1 / intracellular adhesion molecule 1 dependent cell adhesion in CD 40 mediated inhibition of human B lymphoma cell death induced by surface IgM crosslinking . ^^^ Anti CD 40 Abs induced homotypic adhesion of DND 39 cells and rescued them from anti IgM Ab induced cell death . ^^^ Anti CD 40 Abs inhibited anti IgM Ab induced cell death when added within 3 h after stimulation with anti IgM Ab . ^^^ Treatment with Abs against CD11a , CD 18 , or CD 54 inhibited not only the homotypic adhesion but also the inhibition of anti IgM Ab induced apoptosis by anti CD 40 Ab . ^^^ CD11a antisense decreased the surface CD11a expression , the anti CD 40 Ab induced homotypic adhesion , and the inhibitory effect of anti CD 40 Ab on anti IgM Ab induced apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Signaling through CD 40 rescues IgE but not IgG or IgA secretion in 10 linked immunodeficiency with hyper IgM . ^^^ In every 10 HIM patient tested , however , anti CD 40 plus IL 10 did not induce class switching from IgM to IgG or IgA , even in the presence of Staphylococcus aureus Cowan 1 strain ( SAC ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , IL 6 neutralization markedly inhibited the IL 4 induced IgE secretion ( 57 + / 10 % ) as well as the IgG and IgM production resulting from AgR and CD 40 cotriggering ( 49 + / 16 . 5 and 29 . 5 + / 4 . 5 % , respectively ) . ^^^ Blocking IL 10 inhibited the IgA secretion ( 25 + / 2 . 7 % ) obtained after CD 40 activation and the production of IgG , IgA , and IgM ( 24 . 1 + / 5 . 6 , 25 + / 8 , and 42 + / 6 . 5 % , respectively ) by B lymphocytes undergoing dual ligation of CD 40 Ag and AgR . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cross linking of membrane IgM and membrane IgG , but not of CD 40 , leads to the tyrosine phosphorylation of Cbl . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 13 induced proliferation and IgM , total IgG , IgG 4 , and IgE synthesis when total fetal BM cells or highly purified s mu+ , CD10+ , CD19+ fetal B cells were cultured in the presence of anti CD 40 mAb or activated CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B cell activation by crosslinking of surface IgM or ligation of CD 40 involves alternative signal pathways and results in different B cell phenotypes . ^^^ Taken together these results are interpreted to mean that there is a single population of small resting B cells that can respond to either T independent type 2 ( surface IgM ) or T dependent ( CD 40 ) mediated activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Neither IL 3 nor GM CSF stimulates significant IgM secretion by B cells activated through the CD 40 signaling pathway alone , although the combination of CD 40 and membrane Ig signaling leads to a strong enhancement of the IL 3 + GM CSF mediated IgM synthesis above that obtained with membrane Ig signaling alone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Purified B cells did not express CTLA 4 when mitogenically activated with alpha IgM and CD 40 Ab , but did express the molecule when cultured in the presence of membranes from activated T cells , which suggests that induction of CTLA 4 expression on B cells was dependent on direct cell cell contact of B lymphocytes and activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Production of IgM , IgG and IgA was induced from human blood B lymphocytes by culturing with a CD 40 MoAb and IL 2 for 9 days . ^^^ Anti IgD or anti IgM MoAbs enhanced the proliferation of B cells induced by anti CD 40 antibody , but immunoglobulin production was not enhanced . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Modulation of anti IgM induced B cell apoptosis by Bcl xL and CD 40 in WEHI 231 cells . ^^^ In contrast to that observed with Bcl xL , CD 40 stimulation was efficient in inhibiting both cell cycle arrest and apoptosis after IgM cross linking regardless of the binding avidity of the anti IgM Ab . ^^^ Moreover , activation through IgM receptors but not CD 40 induced up regulation followed by rapid down modulation of Bcl xL . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the absence of other stimuli , anti CD 40 and IL 4 induced the secretion of IgM , IgG , and IgE by purified human peripheral blood B cells . ^^^ In the presence of SA , anti CD 40 and IL 4 induced the secretion of IgM , IgG 1 , IgG 2 , IgG 3 , and IgG 4 in addition to IgE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
While resting CD5+ B cells proliferated and produced IgM molecules in response to a mu Ab , IL 4 and CD 40 mAb as well as to Staphylococcus aureus Cowan strain 1 ( SAC ) and IL 2 , resting CD 5 B cells , which were co purified in the same 60 % Percoll fractions , consistently failed to respond . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
On the other hand , these novel mAb could inhibit the co stimulatory effect of the previously described anti CD 40 mAb S2C6 on anti IgM induced human B cell proliferation . ^^^ Similarly , a fusion protein , consisting of the extracellular portion of CD 40 and human IgM constant domains CH 2 , CH 3 and CH 4 , could completely inhibit EL4B5 induced human B cell proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Addition of soluble CD 40 human IgM fusion protein ( sCD 40 ) to FDC B cell cultures also did not interfere with FDC mediated B cell rescue . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the past year , researchers have identified the genes responsible for 10 linked severe combined immunodeficiency ( encoding a cytokine receptor protein ) , 10 linked agammaglobulinemia ( encoding a cytoplasmic tyrosine kinase ) and 10 linked hyper IgM syndrome ( encoding the ligand for CD 40 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The molecular origin of 10 linked hyper IgM syndrome has recently been identified as a defect in the ligand of CD 40 , gp 39 , a protein expressed on the surface of activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this paper , we report that in response to interleukin 10 ( IL 10 ) , anti CD 40 activated tonsillar surface IgD+ ( sIgD+ ) B cells are induced to secrete large amounts of IgM , IgG 1 , and IgG 3 but neither IgG 2 nor IgG 4 . ^^^ Cord blood purified B cells and lymphocytes from Hyper IgM patients also produced IgG 1 and IgG 3 after culture with anti CD 40 and IL 10 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Phenotypic analysis using monoclonal antibodies to B 220 , IgM , IgD , c kit , IL 7 receptor , MHC class 2 , AA4 . 1 , CD 19 , CD 23 , CD 25 , CD 40 and CD 43 shows that B lymphopoiesis in the bone marrow is dramatically altered and the number of pro / pre B and immature B cells is significantly increased . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We compared antigen pulsed B cells that had been activated through different membrane receptors ( IgM , CD 14 and CD 40 ) for their ability to induce T cell proliferation and to respond to T cell help . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Bcl xL but not Bcl 2 is rapidly induced in peripheral B cells upon surface immunoglobulin M ( IgM ) cross linking , CD 40 signaling , or LPS stimulation . ^^^ Induction of Bcl xL after signaling through surface IgM and CD 40 appears to provide mature B cells with an additional protective mechanism against apoptotic signals associated with antigen induced activation and proliferation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Both ionomycin and anti IgM trigger activation of CPP 32 and cleavage of PARP prior to the onset of apoptosis ; this process is abrogated by treatment with anti CD 40 and is independent of Bcl 2 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Co ligation of surface IgM and CD 40 on naive B lymphocytes generates a blast population with an ambiguous extrafollicular / germinal centre cell phenotype . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation mediated by RP 105 but not CD 40 makes normal B cells susceptible to anti IgM induced apoptosis : a role for Fc receptor coligation . ^^^ In contrast to RP 105 activated B cells , blast cells that had been activated by CD 40 ligation were unaltered by anti IgM . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Addition of S oligo 1 together with IL 4 also decreased the up regulated but not constitutive levels of IL 4R on peripheral blood B cells without affecting the concomitant enhancement of CD 23 , CD 40 , HLA DR and surface IgM expression , indicating that its effect is specific for IL 4R up regulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , in vitro generated DC interact directly with B cells , activated through their CD 40 antigen , leading to enhanced growth , differentiation ( IgM production ) and preferential isotype switch towards IgA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Tumour dormancy and cell signalling 3 : Role of hypercrosslinking of IgM and CD 40 on the induction of cell cycle arrest and apoptosis in B lymphoma cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Additionally , B lymphocytes cultured in the CD 40 system in the presence of human IL 10 , produce high level of IgM , IgG and IgA , which are further increased by addition of SAC . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Splenic B 1 , but not B 2 cells from young ( NZB 10 NZW ) F 1 mice spontaneously produced substantial amounts of IgM including IgM anti DNA antibodies , and the levels increased in case of stimulation with anti CD 40 mAb alone , or to a greater extent with the mAb plus IL 4 and IL 5 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we demonstrate that CD 40 or IgM receptor stimulation of primary B cells results in transactivation of this enhancer . 4 Hydroxy 3 nitrophenylacetyl ( NIP ) BSA induction of a K 46 B cell line expressing a chimeric NIP specific CD 40 single chain receptor results in a ligand receptor dependent response of a 3 ' enhancer ETS / AP 1 minimal promoter construct . ^^^ Gel retardation analysis and genomic footprinting experiments reveal that CD 40 or IgM induction recruits NFAB ( nuclear factors of activated B cells ) to the ETS / AP 1 motif . ^^^ While IgM signalling recruits c Fos , JunB and Elf 1 ( NFAB 1 ) , only JunB and Elf 1 were observed following CD 40 signalling ( NFAB 2 ) . ^^^ We propose a model whereby CD 40 and IgM receptor mediated signalling converge in the process of 3 ' enhancer activation in B lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Role of LFA 1 / ICAM 1 dependent cell adhesion in CD 40 mediated inhibition of anti IgM antibody induced B cell death ] . ^^^ To investigate the role of CD 40 transduced signals in surface IgM ( sIgM ) mediated B cell death , we used a human B cell line ( DND 39 ) . ^^^ Anti CD 40 Abs induced homotypic adhesion of DND 39 cells and rescued them from anti IgM Ab induced apoptosis . ^^^ Treatment with Abs against LFA 1 / ICAM 1 inhibited not only the homotypic adhesion but also the inhibition of anti IgM Ab induced apoptosis by anti CD 40 Ab . ^^^ Otherwise , CD11a antisense decreased the inhibitory effect of anti CD 40 Ab on anti IgM Ab induced apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation of mitogen activated protein kinases via CD 40 is distinct from that stimulated by surface IgM on B cells . ^^^ Analysis of ERK activities in the B cell lymphoma cell line WEHI 231 , which shows an increase in DNA synthesis or arrest of the cell cycle by cross linking of CD 40 or surface IgM ( sIgM ) cross linking , respectively , indicated that one of the ERK isoforms , ERK 2 , was preferentially and rapidly activated after CD 40 cross linking . ^^^ Co stimulation of WEHI 231 cells with anti CD 40 mAb rescues the cells from anti IgM mediated apoptosis , whereas this co stimulation resulted in activation of ERK isoforms comparable to that in sIgM stimulation , without a synergistic effect . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Dual BCR and CD 40 ligation with anti immunoglobulin mAb ( anti kappa + anti lambda light chains ) but not with SA induced a similar reduction in IgM production . ^^^ The combination anti kappa and anti lambda with anti CD 40 showed a long term suppression of IgG and IgM production ( at least 14 days ) , while anti kappa or anti lambda alone , or SA , allowed a moderate recovery of immunoglobulin production by day 14 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Notably , Sia6LacNAc deficient mice display reduced serum IgM levels , impaired B cell proliferation in response to IgM and CD 40 crosslinking , and attenuated antibody production to T independent and T dependent antigens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Assays of in vitro immunoglobulin production by lymphocytes showed that IgM was produced normally and that IgE but not IgG or IgA production was rescued by signaling through CD 40 on B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Neutralization of endogenous IL 10 does not alter growth of CD 40 activated B cells but inhibits their IgG , IgA , and IgM secretion , especially when costimulated with SAC particles . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The contribution of specific B T cell contact mediated events to the diminished cord B cell response in this system , using mAbs to CD 40 , CD 28 , CD 80 , and CD 72 , were investigated , as well as regulation of B cell Ig production by cytokines . alphaCD 72 ligation increased cord B cell activation and IgM production , but did not affect adult B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we further show that in the presence of surrogate activated T cells in the form of CD 40 ligation and IL 2 , IDC enhance the proliferation of naive B cells and induce their differentiation into plasma cells producing IgM . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of surface immunoglobulin M ( IgM ) induced cell death and this was blocked by CD 40 mediated signal in a mouse B cell line , WEHI 231 . ^^^ In order to define which region of CD 40 and how CD 40 mediated signal are involved in the blocking of IgM induced cell death , we generated several human CD 40 transfectants . ^^^ We demonstrated that a 10 amino acid segment in the conserved cytoplasmic region of CD 40 mediated rescue from IgM induced cell death and was involved in the activation of c Jun aminoterminal kinase ( JNK ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In contrast , IgG production induced by CD 40 MAb , IgM MAb , and IL 10 was in the normal range . ^^^ In line with this , CD 70 upregulation on B cells from HIV infected individuals was impaired after stimulation in vitro by activated T cells but not after stimulation with CD 40 MAb and IgM MAb . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , peripheral B cells displayed normal responses to IL 4 , IgM , and CD 40 cross linking . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Modulation of E2F activity via signaling through surface IgM and CD 40 receptors in WEHI 231 B lymphoma cells . ^^^ Stimulation of the phenotypically immature B cell lymphoma WEHI 231 with anti IgM induces G 1 arrest followed by apoptotic cell death , which can be reversed by stimulation via the CD 40 receptor . ^^^ We found that anti IgM treatment induces the appearance of an inhibitory DNA binding complex containing the pRB related pocket protein p 130 together with E2F and a concomitant decrease in `` free ' ' E2F , consisting of E2F1 and its partner DP 1 ; these effects were reversed following stimulation via CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon stimulation , resting B cells from young NZB and NZB / W mice demonstrate increased proliferation , IgM secretion , or enhanced expression of costimulatory molecules in response to a variety of different T cell derived stimuli , including cytokines and signals generated through CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The specificity of these inhibitors is demonstrated by their failure to prevent responses to LPS , PMA , or ligation of CD 40 or IgM . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Triggering of CD 40 led to an augmentation of IL 6 expression and IgM secretion . ^^^ Blocking the binding of IL 6 to its cellular receptor through the use of inhibitory monoclonal antibodies inhibited CD 40 induced IgM secretion , suggesting a possible autocrine role of IL 6 for the differentiation of a CD 40 activated B cell hybridoma . ^^^ Co triggering with CD 40 and B cell receptor or activation through CD 40 and IL 4 led to a synergistic augmentation of IL 6 expression as well as additive IgM secretion ; this was followed by a marked decrease in the expression of B cell surface markers on the cell membrane . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Costimulation of mononuclear cells from most of the patients induced no to low levels of class switching from IgM to IgG and IgA with Staphylococcus aureus Cowan strain ( SAC ) plus IL 2 or anti CD 40 mAb ( anti CD 40 ) plus IL 10 . ^^^ Costimulation with SAC plus IL 2 plus anti CD 40 plus IL 10 yielded secretion of significant levels of IgG in addition to IgM , but not IgA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Costimulation with either IL 4 or anti CD 40 interferes with anti IgM stimulated ERK 2 activation . ^^^ N acetyl L cysteine pretreatment , which blocks CD 40 mediated JNK activation , does not affect the ability of CD 40 to inhibit anti IgM mediated ERK 2 activation and apoptosis . ^^^ Together , these data suggest that JNK activation is not required for CD 40 inhibition of surface IgM induced cell death and that ERK 2 plays an active role in mediating anti IgM induced apoptosis of WEHI 231 B cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , a panel of rat anti mouse B cell mAb , including Ab directed at surface IgM Id , CD 19 , CD 22 , CD 40 , CD 74 , and MHC class 2 , has been investigated in the treatment of two syngeneic mouse B cell lymphomas , BCL 1 and A 31 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Disruption of CD 40 and its ligand , CD 154 leads to immunodeficiency as in the case of hyper IgM syndrome . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IgM levels were significantly reduced by mAb against CD 40 , CD 48 , CD 58 and CD 80 . ^^^ Only mAb against CD 80 and CD 48 significantly reduced IgM production , the reduction of IgM production by anti CD 40 mAb was less than in the presence of T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In vitro , c Rel ( / ) B cells proliferate poorly upon ligation of their surface IgM or CD 40 receptors or when stimulated with either lipopolysaccharide ( LPS ) or T cell help . ^^^ When c Rel ( / ) B cells are co stimulated with either anti IgM and CD 40 or LPS and CD 40 , they are rendered capable of progressing through the cell cycle . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , following CD 40 stimulation alone , IFN gamma was relatively ineffective at stimulating high rate synthesis of any non IgM isotype . ^^^ Dual cross linking via CD 40 and the BCR resulted in decreased synthesis of IgM with a concomitant increase in IgA and similar levels of IgG 2 production to those obtained via the BCR alone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we review the implications of several signals mediated by receptors , such as surface IgM , CD 6 and CD 40 , for the B CLL cell survival , together with data on gene modulation in relation to the apoptosis process in B CLL cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In costimulation with antibodies to IgM ( but not to CD 40 ) , some of these induced the cells to proliferate ( but not to differentiate ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thymic B cells expressed B cell markers such as IgM , Fc gamma receptor , CD 44 , heat stable antigen , LFA 1 and CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BALM 18 may be useful for analyzing both the mechanisms of anti IgM induced apoptosis and signaling during the inhibition of apoptosis by CD 40 or BST cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This observation is consistent with the detection of normal CD 40 induced monocyte activation in patients with CD 40 ligand+ hyper IgM syndrome in whom a defect in CD 40 induced B cell activation has been reported . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Modulation of E2F activity in primary mouse B cells following stimulation via surface IgM and CD 40 receptors . ^^^ Since signals via CD 40 and the B cell receptor are known to synergize to induce B cell activation , we have analyzed the pocket protein / E2F complexes in mouse B lymphocytes following stimulation by anti IgM , anti CD 40 , alone or together . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Triggering of CD 40 led to an augmentation of IgM secretion as well as IL 6 expression . ^^^ Blocking the binding of IL 6 to its cellular receptor through the use of inhibitory antibodies inhibited CD 40 induced IgM secretion , suggesting a possible autocrine role of IL 6 for CD 40 induced differentiation of this B cell hybridoma . ^^^ Addition of IL 4 or Br MRBC augmented IL 6 expression as well as IgM secretion by CD 40 activated MRL159 . 5 cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Both CpG ODN and engagement of CD 40 protects WEHI 231 murine B lymphoma cells from apoptosis induced by antibody to surface IgM , and both agents induce interleukin 6 ( IL 6 ) production by these cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Distinct subcellular localization and substrate specificity of extracellular signal regulated kinase in B cells upon stimulation with IgM and CD 40 . ^^^ We and others previously observed that IgM and CD 40 stimulation in murine B cells resulted in activation of extracellular signal regulated kinase ( ERK ) , a subfamily of mitogen activated protein kinase . ^^^ The present study demonstrated that ERK was rapidly phosphorylated and translocated to the nucleus in murine B cells upon stimulation with CD 40 , whereas it was preferentially localized within the cytosol after stimulation with IgM , suggesting that signaling through CD 40 and IgM differentially regulates ERK subcellular localization . ^^^ Costimulation with CD 40 and IgM ( CD40 / IgM ) resulted in subcellular localization of ERK within the cytosol , supporting the notion that stimulation with IgM delivers the signal responsible for inhibition of ERK nuclear transport . ^^^ Consistent with these observations , IgM and CD40 / IgM stimulation resulted in activation of ribosomal S 6 kinase , which is a cytoplasmic substrate for ERK , whereas CD 40 stimulation had little effect on its activity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Anti CD 40 monoclonal antibody ( MAb ) has been shown earlier to costimulate with IgM or phorbol esters resting B cells to proliferate , differentiate , secrete immunoglobulins , and switch isotype . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cyclin D 2 is the only D type cyclin expressed in mature mouse B lymphocytes , and its expression is associated with retinoblastoma protein ( pRB ) and pRB related protein phosphorylation and induction of E2F activity , as B cells enter the cell cycle following stimulation via surface IgM and / or CD 40 . ^^^ Here we show that B lymphocytes from cyclin D 2 ( / ) mice can proliferate in response to anti IgM and anti CD 40 , but the time taken to enter S phase is longer than for the corresponding cyclin D 2 ( + / + ) cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , B cells stimulated through both the CD 40 receptor and membrane IgM appear to mediate a stronger protective effect on T cells by virtue of their resistance to FasL mediated cytolysis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Despite expressing CD 95 , the CD 40 activated CLL target cells were found to be resistant to apoptosis induced by CH 11 , an IgM CD 95 monoclonal antibody ( mAb ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The RLN of both naive and SIV infected macaques contained high numbers of CD 68 + MHC II+ macrophages and cells expressing the co stimulatory molecules B 7 2 and CD 40 , as well as IgM + MHCII+ and IgM + CD40+ B cells , indicating maintenance of antigen presentation capacity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We measured concentrations of IgD ( and IgE and IgM as controls ) in culture supernatants of peripheral blood mononuclear cells ( PBMC ) from 60 normal donors as well as mononuclear cells from 10 tonsils following culture in the absence or presence of CD 40 mAb and cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To determine whether CD 40 ligation influences the molecular and selective mechanisms that govern the development of the human Ig light chain repertoire , analysis of the Vkappa and Vlambda repertoires of CD19+ B cells obtained from a patient with 10 linked hyper IgM syndrome ( XHIM ) and a nonfunctional CD 154 was carried out . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using cultures of resting primary mouse B cells stimulated in vitro in various combinations with IL 4 , anti mu F ( ab ' ) 2 or anti CD 40 in the absence or presence of lipopolysaccharide , we show that IgM secretion and the expression of Blimp 1 is either not induced or even suppressed by B cell receptors ( BCR ) or CD 40 ligation and by IL 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , the Em 2 ( G 11 ) antigen induced a specific IgM and IgG response in T cell deficient athymic nude , TCRbeta ( / ) , major histocompatibility complex class 2 ( MHCII ) ( / ) ( CD 4 deficient ) , and CD 40 ( / ) mice . ^^^
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Although neither adult CD 27 ( ) nor cord blood B cells , which showed the unmutated Ig 5 region genes , produced IgG , IgM , or IgA in response to conventional stimuli , they produced IgG and IgM but not IgA in the presence of Staphylococcus aureus Cowan strain ( SAC ) + interleukin 2 ( IL 2 ) + IL 10 + anti CD 40 mAb + CD 32 transfectants ( CD40 / CD32T ) . ^^^
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Furthermore , resting B cells also expressed IL 13Ralpha1 , and in the presence of IL 13 produced increased amounts of IgM in response to in vitro CD 40 stimulation . ^^^
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RESULTS : IgM anti CCP autoantibodies were induced in PB B cells from healthy controls and RA patients following coculture with activated T cells or application of the CD 40 activation system , whereas no production could be detected when PB B cells were cultured in the absence of a stimulus . ^^^
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CD 40 expression is induced by the introduction of IgM receptor on the surface of pro B cell line NFS 70 . ^^^
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Similar to IgM production , IgM RF production was shown to be dependent on CD 40 cross linking . ^^^ However , activation of PB B cells in the CD 40 system in the presence of IL 2 , IL 4 , IL 10 , combinations of these cytokines or supernatant of anti CD 3 stimulated T cells failed to induce detectable IgM RF , whereas total IgM production was considerable . ^^^
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But CpG ODN stimulation up regulated the expression of MHC 1 , MHC 2 , CD 40 , ICAM 1 molecules in A 20 cells , enhanced the antigen uptake ability of A 20 cells , and promoted A 20 cell production of IgM and IgG . ^^^
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Role of TNF receptor associated factor 2 in the activation of IgM secretion by CD 40 and CD120b . ^^^ Here we demonstrate that CD 40 induced TNF stimulates IgM production through CD120b and that CD120b signaling is required for optimal CD 40 induced IgM secretion . ^^^ Furthermore , although both CD 40 and CD120b can bind TRAF 2 , TRAF 2 dependent CD 40 signals can not substitute for TRAF 2 dependent CD120b signals in the activation of IgM secretion . ^^^ Our results indicate a potentially important role for CD120b in the activation of IgM secretion and that TRAF 2 is used by CD 40 and CD120b in distinct ways . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Most of the surface membrane antigens ( CD 5 , CD11c , CD 19 , CD 20 , CD 21 , CD 22 , CD 23 , CD 25 , CD 40 , CD 45 , VD79b , CD 80 , CD 95 , CD 122 , CD 124 , CD 126 , CD 130 , CD 154 , IgM , and IgD ) showed a similar expression pattern in both UM CLL and M CLL patients . ^^^
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As such , we examined by flow cytometric analysis tumor cells from a large series of patients with the histopathlogical diagnosis of lymphoplasmacytic lymphoma and the clinicopathological diagnosis of WM for expression of the serotherapy target antigens CD 20 , CD 22 , CD 40 , CD 52 , IgM , MUC 1 core protein , and 1D10 . ^^^ These studies demonstrated antigen expression on > or=50 % of bone marrow tumor cells ( CD 19 ( + ) , kappa / lambda light chain restricted ) , respectively , from patients as follows : CD 20 ( 98 . 3 % ) , CD 22 ( 88 . 3 % ) , CD 40 ( 83 . 3 % ) , CD 52 ( 77 . 4 % ) , IgM ( 83 . 3 % ) , MUC 1 core protein ( 57 . 8 % ) , and 1D10 ( 50 % ) . ^^^
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We show that P . carinii specific IgM is not sufficient to mediate clearance of P . carinii from the lungs since CD 40 deficient mice produced normal levels of specific IgM , but were unable to clear the organisms . ^^^
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We have recently identified 2 patients with a rare autosomal recessive form of hyper IgM disease , known as HIGM 3 , caused by mutations in the CD 40 gene . ^^^ These patients had opportunistic infections observed on 10 linked hyper IgM syndrome ( HIGM ) , suggesting that the CD 40 CD40 ligand interaction is important for promoting T cell mediated immunity . ^^^
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The frequencies of somatic hypermutation in cB 2 and cB 3 were comparable levels studied by VH 5 . cB 2 did not shift to cB 3 in vitro by the stimuli such as via B cell receptor or CD 40 . cB 2 produced large amounts of IgM predominantly and promptly , which is in accordance with the known characteristics of memory B cells . ^^^
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We demonstrate that TRAF 2 participates in synergy between CD 40 and B cell antigen receptor signals , and in CD 40 mediated , TNF dependent IgM production . ^^^
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CD 27 synergizes with CD 40 to induce IgM , IgG , and IgA antibody responses of peripheral blood B cells in the presence of IL 2 and IL 10 . ^^^ CD 40 , a member of the tumor necrosis factor receptor ( TNFR ) family , promotes IgM , IgG , and IgA antibody ( Ab ) synthesis in combination with a variety of cytokines . ^^^ The anti CD 40 mAb substantially enhanced IgM , IgG , and IgA production in combination with IL 2 and IL 10 , whereas anti CD 27 mAb augmented it only marginally , as assessed by enzyme linked immunosorbent assay . ^^^ The addition of anti CD 27 mAb enhanced the anti CD 40 mediated IgM , IgG , and IgA antibody production only when both IL 2 and IL 10 were present in the culture . ^^^
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CD 40 ligation compensated for CD 22 deficiency by restoring lymphoblast development , proliferation , c Myc and CUL 1 expression , and protein ubiquitination / degradation in IgM stimulated B 6 CD22 ( / ) B cell cultures . ^^^
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From the present results it is possible that reduction of N glycosylation of the heavy chain of IgM by SW treatment may reduce anti IgM induced growth inhibition , and reduction in anti IgM induced growth inhibition due to altered N glycosylation may enhance CD 40 CD40L mediated cell survival through TRAF 2 which interacts with both IgM and CD 40 in HBL 2 cells . . ^^^
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IL 5 stimulation of wild type B 1 cells , but not IL 5Ralpha ( / ) B 1 cells , enhances CD 40 expression and augments IgM and IgG production after stimulation with anti CD 40 mAb . ^^^
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A 12 month old female presented at Ege University , Faculty of Medicine , Department of Paediatrics with complaints of fever , diarrhoea , respiratory distress and growth development retardation was diagnosed with CD 40 deficiency ( Hyper IgM Type 3 ) . ^^^
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CD 40 and CD120b mediated IgM secretion were partly TRAF 2 dependent , but only CD 40 required TRAF 2 for c Jun N terminal kinase activation . ^^^
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PATIENTS AND METHODS : We studied the expression of CD40L , CD 40 and made a mutation analysis of the CD40L gene in 3 males of 2 unrelated Chilean families diagnosed as a possible syndrome of hyper IgM and 3 relatives . ^^^
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The in vivo critical role of CD 40 CD154 interaction on B cell differentiation and isotype switching is provided by the discovery that mutations in either CD 40 or CD 154 gene cause the hyper IgM syndrome , termed HIGM 3 or HIGM 1 , respectively , characterized by very low levels of serum IgG , IgA , and IgE , with normal or elevated IgM , associated with a defective germinal center formation . ^^^ In this article , we present a comprehensive overview of the clinical , genetic , and immunological features of patients with hyper IgM syndrome due to CD 40 mutations . . ^^^
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The expression of 23 surface membrane antigens ( CD 5 , CD11c , CD 19 , CD 20 , CD 21 , CD 22 , CD 23 , CD 25 , CD 27 , CD 38 , CD 40 , CD 45 , CD45RA , CD45RO , CD79b , CD 80 , CD 95 , CD 124 , CD 126 , CD 130 , FMC 7 , IgD , and IgM ) was studied by flow cytometry at days 0 and 4 . ^^^
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Mature B 2 cells expressing surface IgM and IgD proliferate upon stimulation by CD 38 , CD 40 or lipopolysaccharide ( LPS ) and differentiate into IgG 1 producing plasma cells in the presence of cytokines . ^^^
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Murine splenocytes were cultured in order to evaluate the expression of CD 40 , CD45RB and CD 69 on B lymphocyte , and IgG and IgM were quantified in the culture supernatant . ^^^
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BANK deficient mice displayed enhanced germinal center formation and IgM production in response to T dependent antigens , whereas this phenotype was blocked in CD 40 BANK double knockout mice . ^^^
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Switching from IgM to IgG and IgA is essential for antiviral immunity and requires engagement of CD 40 on B cells by CD40L on CD 4 ( + ) T cells . ^^^
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Cross linking surface IgM reduced LCK expression whereas cross linking surface CD 40 appeared to have the opposite effect . ^^^
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Studies with human B cells show that the binding of CD 154 ( gp 39 , CD40L ) to CD 40 recruits TNF receptor associated factor 2 ( TRAF 2 ) and TRAF 3 to the receptor complex , induces the downregulation of the nonreceptor associated TRAFs in the cell and induces an increased expression of Fas on the cell surface . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cell help is mainly mediated through ligation of the B cell surface antigen , CD 40 , by its cognate T cell ligand , CD 154 . ^^^
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Antibodies to the ligand for CD 40 ( CD 154 ) have been shown to exert profound effects on the development of cell mediated immune responses in mice . ^^^
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CD 40 and CD 154 in cell mediated immunity . ^^^ CD 40 and CD 154 knockout mice and antibodies to CD 40 and CD 154 have helped to elucidate the role of the CD 40 CD154 system in immune responses . ^^^
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Cells in human atherosclerotic lesions express the immune mediator CD 40 and its ligand CD40L ( also known as CD 154 or gp 39 ) . ^^^
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Stimulation of both bone marrow and synovial RA NLC induced expression of CD 40 and class 2 MHC , but not CD 154 ( CD40L ) or CD 35 . ^^^
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A previous observation that anti CD40L ( CD 154 ) monoclonal antibodies can effectively prevent EAE in this model was confirmed , and extended by demonstrating that CD 40 is expressed by cells of the monocytic lineage infiltrating the spinal cord . ^^^
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We have recently demonstrated that platelets express the ligand of CD 40 ( CD 154 ) within seconds of exposure to agonist , and interact with endothelial cells to participate directly in the induction of an inflammatory response . ^^^ Since platelets are among the first cells involved in haemostasis following tissue injury , our data showing that ligation of CD 40 by CD 154 induces a procoagulant phenotype on vascular endothelial cells suggests that platelets may play an important role in the induction of wound healing . . ^^^
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The CD 40 CD40L ( CD 154 ) interaction plays a pivotal role in the effector mechanisms of allograft rejection . ^^^
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Ligation of CD 40 : CD 154 was partially essential for IL 12 secretion . ^^^
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Major advances have been made in understanding the expression and function of CD 40 and its ligand CD 154 . ^^^
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CD 40 CD 154 interaction in experimental and human disease ( review ) . ^^^ It has clearly emerged that among these signals few cell surface receptor ligand pairs , such as CD 40 and its ligand , CD 154 , are mandatory for the induction of lymphocyte activation . ^^^
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CD 40 expression on graft infiltrates and parenchymal CD 154 ( CD40L ) induction in human chronic renal allograft rejection . ^^^ Given the critical importance of CD 40 CD154 interactions in the development of chronic transplant allograft rejection , the relevance of in situ CD 40 and CD 154 expression was assessed in human chronic renal allograft rejection . ^^^ METHODS : The expression of CD 40 , CD 154 , CD 68 , and T cell receptor ( TCR ) alpha / beta was analyzed by immunohistochemistry . ^^^
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While CD 40 agonism could enhance short term expansion , sustained expansion of CD8+ Tg T cells required bona fide CD 154 expressing CD4+ alloreactive Th cells . ^^^
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While the binding of CD 154 to CD 40 induces the assembly of a CD 40 TRAF receptor complex , IKK is not recruited to this complex . ^^^
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Enhanced expression of CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 and their ligands CD 28 and CD 154 in fulminant hepatic failure . ^^^ To define a possible role for changes in the regulation of antigen presentation in fulminant hepatic failure ( FHF ) , we studied the expression of co stimulatory molecules CD 80 ( B 7 1 ) , CD 86 ( B 7 2 ) , and CD 40 along with their ligands CD 28 and CD 154 . ^^^
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CD 154 is the ligand for the receptor CD 40 . ^^^
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The interaction between B cell molecule CD 40 and T helper surface molecule CD 154 is the key event of B cell ( and other antigen presenting cell ) activation . ^^^
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To determine whether the CP infection and the consequent immune response contribute independently to this morbidity , we infected mice with severe combined immunodeficiency ( SCID ) or with disrupted genes for CD 154 , CD 40 , or interferon gamma ( IFN gamma ) with CP . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In an attempt to understand the mechanisms of cell injury in the inflammatory myopathies , we analyzed the expression of costimulatory molecules , CTLA 4 , CD 28 , CD 86 , CD 40 , and CD 154 as well as HLA class 1 , HLA class 2 , and ICAM 1 in normal muscle and in muscle biopsies from patients with polymyositis ( PM ) or dermatomyositis ( DM ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Binding of CD 40 by CD 154 expressed on activated T cells is a pivotal event in T cell help to B cells , macrophages , and other antigen presenting cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blocking CD 40 CD 154 and CD80 / CD86 CD 28 interactions during primary allogeneic stimulation results in T cell anergy and high IL 10 production . ^^^ According to more recent data , interruption of the CD 40 CD 154 interaction might complement the effect of CTLA 4lg and induce graft acceptance . ^^^ We conclude that the simultaneous blocking of the CD 40 CD 154 and CD80 / CD86 CD 28 interaction during allogeneic T cell activation induces T cell anergy . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
An important co stimulatory pathway for most immune responses is mediated by the interaction of CD 40 on antigen presenting cells with CD 154 ( CD40L ) on host T cells . ^^^
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B cell expression of CD 154 was induced by engaging surface Ig or CD 40 by signals that predominantly involved activation of AP 1 / NF AT and NF kappaB , respectively . ^^^ In addition , mAb to CD 154 inhibited proliferation induced by engaging sIg or CD 40 , indicating the role of up regulation of this molecule in facilitating B cell responsiveness . ^^^
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This survival effect could be reproduced simply by incubating IPP follicular B cells with soluble and cell membrane expressed CD 154 , an observation consistent with the demonstrated presence of CD 40 and CD 154 on porcine IPP follicular B cells and activated T cells , respectively . ^^^
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Membrane bound CD 154 , but not CD 40 specific antibody , mediates NF kappaB independent IL 6 production in B cells . ^^^ Engagement of CD 40 on either a B cell line or normal mouse splenic B cells with a membrane bound form of CD 154 ( also known as CD40L or gp 39 ) induced IL 6 secretion as well as up regulation of IL 6 mRNA , but cross linking CD 40 with agonistic anti CD 40 mAb did not , although these mAb induce many other CD 40 activation events , including the nuclear translocation of the transcription factor NF kappaB . ^^^ Thus , CD 40 mediated IL 6 induction is independent of NF kappaB activation and the binding of TRAF 2 and 3 , but CD 40 must be engaged by trimeric CD 154 on cell membranes to activate production of IL 6 . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Orbital fibroblasts , unlike most fibroblasts , express CD 40 and when that surface receptor is cross linked with CD 154 , its natural ligand , a number of inflammation related genes are activated . ^^^
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The present review focuses on the role of two receptor ligand pairs : CD 28 and cytotoxic T lymphocyte associated antigen 4 , which interact with the B 7 ligands ( CD 80 , CD 86 ) ; and CD 40 , which interacts with the CD40L ( CD 154 ) . ^^^
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Recent studies , for example , indicate that the stealth like phenotype of leukemia cells can be reversed through transfer of genes such as the one encoding CD 154 , the ligand for CD 40 . ^^^
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CD 40 was usually strongly expressed in SC at all KS stages as well as in EC of non involved tissue whereas CD40L ( CD 154 ) was not demonstrable . ^^^
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CD 154 is an activation induced CD4+ T cell surface molecule that interacts with CD 40 on antigen presenting cells ( APC ) and upregulates the key costimulatory molecules , CD 80 and CD 86 . ^^^
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Functional activity of CD 40 antibodies correlates to the position of binding relative to CD 154 . ^^^
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There was no difference between the acquisition of CD 69 and CD 154 by mesenteric lymph node T cells of C . parvum infected animals with intact or disrupted CD 40 CD154 pathways . ^^^
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Using human monocytes and the human monocytic cell line THP 1 , we examined signal transduction events induced by CD 40 engagement with its ligand , CD 154 . ^^^
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Expression of the costimulator molecules , CD 40 and CD 154 , on lymphocytes from neonates and young children . ^^^ Differential expression of the costimulator molecules CD 40 and CD 154 on neonatal lymphocytes may be one explanation for limited T dependent antibody responses in human neonates . ^^^ These results show some differences in expression of CD 40 and CD 154 between neonatal and adult lymphocytes , but do not directly account for the relative deficiencies of humoral immunity in neonates . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We now show that the CD 40 CD40L ( CD 154 ) interaction is involved in the induction of inducible nitric oxide synthase ( iNOS ) expression during adoptive immunotherapy ( ADI ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blockade of CD 40 : CD 154 interactions between APCs and T cells in vivo induces T cell tolerance to host alloantigen and dramatically reduces GVHD . ^^^
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As we have previously shown , CL 01 cells are IgM+ IgD+ and effectively mutate the expressed Ig VHDJH and 5 lambda J lambda genes and switch to IgG , IgA , and IgE upon B cell receptor engagement and contact with CD4+ T cells through CD 40 : CD 154 and CD 80 : CD 28 coengagement . ^^^
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In addition , the anti CD40L ( CD 154 ) monoclonal antibody ( mAb ) , which can interfere with the interaction of CD 154 on T cells and CD 40 on APCs , can induce long term graft survival in the murine model . ^^^
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Analysis of CD 154 and CD 40 expression in native coronary atherosclerosis and transplant associated coronary artery disease . ^^^ To investigate whether CD 154 CD40 interactions might be involved in the pathogenesis of CA or TCAD we performed immunohistochemical studies of CD 154 and CD 40 expression on frozen sections of coronary arteries obtained from cardiac allograft recipients with CA ( n=10 ) or TCAD ( n=9 ) . ^^^
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Otherwise it was indistinguishable from the murine parent antibody in its ability to bind to CD 154 , block CD 154 binding to CD 40 and inhibit T cell dependent B cell differentiation . ^^^
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RESULTS : CD 20 , CD 22 , CD 24 , CD 40 , CD79a , CD79b , FMC 7 , CD11a , CD 18 , CD 44 were positive in the whole B cell population , whereas CD 10 , CD 86 , CD 103 , CD 154 and FasL were almost absent from the B lymphocyte population . 75 % were IgD positive . ^^^
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METHODS : Peripheral blood mononuclear cells ( PBMC ) were obtained from patients with active disease and activated during 18 h with an anti CD 3 monoclonal antibody in the presence or absence of blocking antibodies to CD 154 or CD 40 . ^^^ Blocking experiments with both anti CD 40 and anti CD 154 Mab showed a significant reduction in the proportion of PB B cells expressing CD 23 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It has been shown that engagement of CD 40 on vascular endothelium by CD 154 on platelets and CD 154 bearing cell lines leads to the induction of adhesion molecule expression . ^^^ Having cloned porcine CD 40 , and shown that it is capable of binding human CD 154 , we investigate whether human CD 154 can activate porcine endothelial cells ( EC ) through CD 40 ligation . ^^^ RESULTS : Human and pig EC were shown to express CD 40 by flow cytometry by using soluble human CD 154 ( CD154Ckappa ) . ^^^ CONCLUSIONS : A number of cells can express CD 154 , including T cells , natural killer cells , and platelets , and these could signal graft EC through the CD 40 pathway . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
LCLs strongly expressed CD 40 and CD 70 on their surface , but marginal or no CD 154 , CD 27 , OX 40 ( CD 134 ) and CD 134 ligand . ^^^
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An integral and necessary part of a T cell immune response involves triggering of CD 40 on antigen presenting cells ( APC ) by its ligand , CD 154 , on responding T helper ( Th ) cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Until recently , the expression and primary function of the cell surface receptor CD 40 and its ligand CD 154 were considered restricted to B and T lymphocytes , and their interactions required for the thymus dependent humoral response . ^^^
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The role of CD 40 on DC and CD 154 on T cells has been studied by the co adoptive transfer of antigen pulsed DC and TCR transgenic ( Tg ) T cells in vivo . ^^^
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CD 40 is a member of the tumor necrosis factor ( TNF ) family of receptors whose ligand ( CD 154 ) is mainly expressed on the membrane of activated CD 4 positive lymphocytes . ^^^ Genetically modified L cells that express elevated levels of CD 154 ( CD40L cells ) were used to stimulate CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interaction of the CD40L ( CD 154 ) molecule on activated T cells with its receptor , CD 40 , on macrophages and dendritic cells ( DC ) provides a strong signal for interleukin ( IL ) 12 production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We evaluated whether human platelets could directly activate porcine endothelium ( PEC ) , and if so , whether this was mediated by an interaction between platelet bound CD 154 and PEC CD 40 . ^^^ CONCLUSION : Human CD 154 expressed on activated human platelets or on T cells interacts with CD 40 expressed on PEC leading to PEC activation . ^^^ This interaction can be inhibited by a monoclonal antibody directed against CD 154 , suggesting that an interaction between human CD 154 and PEC CD 40 is at least in part responsible for PEC activation seen in delayed xenograft rejection . ^^^
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In contrast , inhibition of signal 2 by anti CD 40 ligand ( CD 154 ) with pathogenic peptide K 2 for EAU ( experimental autoimmune uveoretinitis ) tolerates T lymphocytes specific for K 2 , resulting in amelioration of EAU . ^^^
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The ability of high frequencies of CD 8 T cells to override help was not due to their ability to signal CD 40 via expression of CD 154 . ^^^
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Binding of CD 154 to its receptor , CD 40 , provides costimulation for mature B cell activation and differentiation in response to Ag receptor signals . ^^^ We examined the effects of CD 40 ligation during B cell ontogeny in transgenic mice constitutively expressing CD 154 on B cells ( kappaEP CD 154 ) . ^^^
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Ligation of CD 95 by CD95L expressed on anergic T cells in the absence of CD 40 CD40L ( CD 154 ) interaction are critical parameters in eliciting apoptosis in both DC and responder T cells . ^^^ In conclusion , these findings indicate that the defective activation of CD 40 on DC by CD95L+ CD 154 defective anergic T cells could be the primary event in determining T cell suppression and support the role of CD 40 signaling in regulating both conditioning and survival of DC . . ^^^
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We explored the role of CD 40 CD40L ( CD 154 ) in the severe malaria elicited by Plasmodium berghei anka infection in mice . ^^^
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This review highlights emerging evidence linking atherosclerosis to the CD 40 CD40 ligand ( CD 154 ) pathway . ^^^ CD 40 and CD 154 are highly expressed in atherosclerotic human plaques . ^^^
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Ligation of B cell CD 40 by CD 154 expressed on activated T cells stimulates B cell proliferation , differentiation , isotype switching , upregulation of surface molecules contributing to antigen presentation , development of the germinal center , and the humoral memory response . ^^^
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Phenotypically , LBC cells are CD5+ , CD8+ , CD16+ , CD24+ , CD25+ , CD 2 / dim , CD 3 / dim , TCRbeta / dim , TCRgammadelta , CD 154 , CD 40 , and CD45R . ^^^
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The interaction between CD 40 on dendritic cells ( DC ) and its ligand CD 154 has been recognized to be an important feature in the maturation of DC . ^^^
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NLCs also express CD 14 , as well as CD11b , CD 33 , CD 40 , CD45RO , CD 68 , CD 80 , CD 86 , HLA DQ , and HLA DR , but not CD1a , CD 2 , CD 3 , CD11c , CD 19 , CD45RA , CD 83 , CD 106 , or CD 154 . ^^^
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The CD 40 Signalosome appears to be initiated through autochthonous production and cognate binding of CD 154 ( CD40L , gp 39 ) to CD 40 by the lymphoma cell . ^^^ Constitutive expression of NF kappaB in NHL B can be downregulated by treatment with antibodies to CD 40 or CD 154 that disrupt Signalosomes , inhibit lymphoma cell growth , and induce cell death . ^^^
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We have previously shown that binding of human CD 154 expressed on the Jurkat D1 . 1 cell line to porcine CD 40 on pig aortic endothelial cells ( PAECs ) can lead to up regulation of vascular cell adhesion molecule ( VCAM ) 1 and MHC class 2 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Latent sensitivity to Fas mediated apoptosis after CD 40 ligation may explain activity of CD 154 gene therapy in chronic lymphocytic leukemia . ^^^
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Induction of OVA specific CTL by cross priming requires help from CD 4 T cells , which use CD 154 to signal CD 40 on the APC . ^^^
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CD 154 is transiently expressed by activated T cells and interacts with CD 40 on B cells , dendritic cells , macrophages , and monocytes . ^^^
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METHODS AND RESULTS : In a coculture model of human endothelial cells and a transfected CD 154 positive cell line , engagement of CD 40 on endothelial cells dramatically increased the synthesis of prostacyclin , prostaglandin E ( 2 ) and thromboxane A ( 2 ) . ^^^ Western blot analysis demonstrated that Cox 2 protein was markedly increased in endothelial cells following CD 40 engagement , an effect that was inhibited by pretreatment of cells with an anti CD 154 antibody . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recruitment of CD 154 ( / ) 8 . 3 CD 8 ( + ) CTL was accelerated by CD 154 ( + ) CD 4 ( + ) T cells , by expression of a B7 . 1 transgene in beta cells or by treatment of the mice with CpG DNA or an agonistic anti CD 40 antibody . ^^^ RAG 2 ( / ) mice lost their diabetogenic potential if they lacked CD 154 , even in the presence of CD 154 ( + ) CD 4 ( + ) T cells , B7 . 1 molecules on beta cells , CpG DNA treatment , or systemic CD 40 ligation . ^^^ These results demonstrate the existence of a novel , CD 154 dependent pathway of CD 4 ( + ) T cell activation that is independent of CD 40 mediated activation of APCs . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IDEC , in collaboration with Eisai , is developing IDEC 131 ( E 6040 ) , a humanized monoclonal antibody ( mAb ) against CD 154 , the ligand for CD 40 also called CD40L or gp 39 , for the potential treatment of several autoimmune diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
F5 , a novel hamster anti rat monoclonal antibody to CD 154 , blocks CD 40 dependent T cell costimulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
One of these interactions involves ligation of B cell CD 40 by T cell expressed CD 154 . ^^^
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CD 154 triggers CD 40 on antigen presenting cells , thus inducing antigen presentation to the immune system and production of IL 12 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thus these observations confirm that ovine CD 40 blockade inhibits co stimulation mediated by CD 40 CD40L ( CD 154 ) interactions as has been reported in murine and human studies . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 activation by CD 154 may trigger diverse cellular responses , ranging from proliferation and differentiation to growth suppression and cell death , in normal and malignant cells . ^^^ We found coexpression of CD 40 and CD 154 in most of the renal carcinoma cell lines . ^^^ CD 154 , but not CD 40 expression , significantly correlated with tumor stage . ^^^ CD 40 engagement by CD 154 did not affect apoptosis or survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
PURPOSE : Triggering of CD 40 on antigen presenting cells via its ligand CD 154 is an important event in the initial phase of an immune response against cancer cells . ^^^
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Consequently , the death and survival signalling arms within germinal centres predominantly reside on the timely and controlled expression of Fas and its ligand ( FasL ) , and CD 40 and CD 154 , respectively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent studies indicate that the stealth like phenotype of leukemia cells can be reversed through transfer of genes encoding recombinant membrane stabilized proteins of the tumor necrosis factor ( TNF ) imily , such as the one encoding CD 154 , the ligand for CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The similar levels of insusceptibility of CD 40 ( / ) and CD 154 ( / ) B 6 mice indicate that CD154 / CD40 molecular interactions are required for MAIDS . ^^^ CD 4 ( + ) T and B cells , respectively , provide the CD 154 and CD 40 expression needed for MAIDS induction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , infection of CD 40 ( positive ) myeloma cells with Ad CD 154 , but not an adenovirus encoding an irrelevant transgene , beta galactosidase ( Ad LacZ ) , induced enhanced expression of immune accessory molecules , such as CD 54 , HLA DR and CD 70 . ^^^ In addition , Ad CD 154 infected myeloma cells could activate bystander CD 40 ( positive ) antigen presenting cells to express immune accessory molecules . ^^^ Finally , co infection of CD 40 ( negative ) myeloma cells with Ad CD 154 and an adenovirus encoding CD 40 ( Ad CD 40 ) induced expression of immune accessory molecules and enhanced the MLR stimulatory capacity of transduced myeloma cells . ^^^ Collectively , these results indicate that infection of myeloma cells with Ad CD 154 or Ad CD154 / Ad CD 40 can induce changes in myeloma cells that enhance their ability to induce cellular immune activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 , CD 154 , Bax and Bcl 2 expression in Sjgren ' s syndrome salivary glands : a putative anti apoptotic role during its effector phases . ^^^ CD 40 and CD 154 expression was also investigated , as CD 40 ligation has been suggested to protect cells from Fas mediated apoptosis . ^^^ CD 40 and CD 154 expression was high among SS IMCs , while CD 40 levels were slightly decreased in SS epithelium compared with controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This in vitro culture system involves CD 40 activation of B lymphocytes by its ligand CD 154 in the presence of cytokines . ^^^
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CD 40 related treatment approaches have been considered for the experimental therapy of human leukemias , lymphomas , and multiple myeloma , based on findings that CD 40 binding by its natural ligand ( CD40L ) , CD 154 , led to growth modulation of malignant B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 86 ( + ) major histocompatibility complex class 2 ( + ) ( MHC 2 ) ( + ) cells and CD 28 ( + ) CD 4 ( + ) T cells were decreased by SST treatment , while CD 80 ( + ) MHC 2 ( + ) cells , CD 40 ( + ) MHC 2 ( + ) cells and CD 154 ( + ) CD 4 ( + ) T cells showed no change . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Importantly , CD 40 expression by osteoblasts was functional , as assessed by ligation of this molecule with recombinant , soluble CD 154 . ^^^ Cocultures of activated CD 4 ( + ) T lymphocytes and osteoblasts could interact via CD 40 and CD 154 , since an antibody against CD 40 could block macrophage inflammatory protein 1alpha secretion . ^^^
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Distinct contributions of different CD 40 TRAF binding sites to CD 154 induced dendritic cell maturation and IL 12 secretion . ^^^
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Engagement of CD 40 by its ligand CD 154 induces IL 6 production by B lymphocytes . ^^^
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The CD 40 molecule binds CD40L ( CD 154 ) and is necessary for T dependent antibody responses . ^^^
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METHODS : Antibodies to CD 4 , CD 8 , CD 34 , CD 40 , CD 54 , CD 80 , CD 86 , CD 154 , and HLA DR were used for immunohistochemical analysis of lacrimal gland biopsy specimens obtained from nine patients with cGVHD and five with Sjgren ' s syndrome ( SS ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upregulation of CD 40 CD40 ligand ( CD 154 ) in patients with acute cerebral ischemia . ^^^ CD 154 and P selectin were analyzed on platelets and CD 40 on monocytes during and 3 months after acute cerebral ischemia by double label flow cytometry . ^^^ RESULTS : Our main findings are as follows : ( 1 ) patients with acute cerebral ischemia showed a significant increase of CD 154 on platelets and CD 40 on monocytes compared with controls ; ( 2 ) plasma levels of soluble CD 154 were significantly higher in these patients ; ( 3 ) these patients had significantly higher numbers of prothrombotic platelet monocyte aggregates ; ( 4 ) the chemoattractant MCP 1 was significantly elevated in cerebral ischemia ; and ( 5 ) at 3 months ' follow up , upregulation of CD 154 still persisted in patients with previous acute cerebral ischemia . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Nevertheless , naive and memory cells revealed a nigh identical capacity for acquiring CD 77 : both appeared equally sensitive in this regard , with high level CD 40 engagement via cell bound CD 154 being required for both subsets to achieve the hallmark centroblast phenotype . ^^^
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Activation of CD 40 by CD 154 induces antigen presenting cells ( APC ) to express immune costimulatory molecules , thereby enhancing their APC activity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In situ expression of CD 40 , CD40L ( CD 154 ) , IL 12 , TNF alpha , IFN gamma and TGF beta 1 in murine lungs during slowly progressive primary tuberculosis . ^^^
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Neither acute rejection nor immunosuppressant drug therapy ( cyclosporine or tacrolimus ) correlates with expression of either CD 40 or CD 154 on peripheral blood cells among human cardiac transplant patients . ^^^ In this study we investigated the expression of CD 40 and CD 154 on peripheral blood cells from HT patients so as to determine possible association with AAR . ^^^ Using two color flow cytometry , we determined the expression of CD 40 and CD 154 in 102 samples of peripheral blood taken from 53 adult HT patients and in 17 samples from healthy adult volunteers . ^^^ The percentages of HT patients ' blood samples showing above normal CD 40 or CD 154 expression did not differ significantly from those of controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A Salmonella typhi OmpC fusion protein expressing the CD 154 Trp140 Ser 149 amino acid strand binds CD 40 and activates a lymphoma B cell line . ^^^ Structural models based on the homology of CD 154 with TNF and lymphotoxin indicate that binding to CD 40 involves three regions surrounding amino acids K 143 , R 203 and Q 220 , and that strands W 140 S149 and S 198 A210 are critical for such interactions . ^^^ Binding to CD 40 was established by three variants of enzyme linked immunosorbent assay , a direct binding assay by coating plates with a recombinant CD 40 Fc protein and through two competition assays between OmpC gp 39 and recombinant CD 154 or soluble CD 40 Fc . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 is a cell surface molecule expressed on various cells that is important for the response to activated T cells through CD 154 . ^^^ Ligation of CD 40 by recombinant human CD 154 ( 0 . 5 2 microg / ml ) resulted in increased production of IL 6 , IL 8 , and monocyte chemoattractant protein 1 in SSc fibroblasts in a dose dependent manner , whereas these phenomena were not shown in normal fibroblasts even with the addition of CD 154 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 154 CD 40 induced reactivation of latent HIV 1 infection . ^^^
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Suppression could not be ascribed to TGFbeta , IFNgamma , TNFalpha , CD 48 , or ICAM 1 , or to interactions between CD 40 and CD 154 , or CD 95 and CD 95 ligand . ^^^
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It could be inferred that a possible mechanism of CpG ODN for normalization of senescence associated dysregulation of the Th1 / Th2 balance involves the upregulated expression of CD 154 and CD 40 molecules on immune competent cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Of particular interest are members of the ever growing family of T cell co stimulatory pathways ; the classic co stimulatory pathways are the CD28 / CTLA4 : B 7 1 / 2 and CD 40 : CD 154 , while the ICOS : ICOSL and PD 1 : PD L1 / PD L 2 pathways are novel . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In mice , the binding of CD 40 by CD 154 clearly enhances the activation and differentiation of memory B lymphocytes . ^^^
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Defective ligation of CD 40 by CD40L ( CD 154 ) was responsible for CD 95 mediated and spontaneous apoptosis of DC as well as for a failure of their maturation process . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of B cell CD 40 by CD 154 , mainly expressed on activated T cells , stimulates B cell proliferation , differentiation , isotype switching , up regulation of surface molecules contributing to antigen presentation , development of the germinal center , and the humoral memory response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The results obtained indicate that the interaction of CD 40 with soluble CD 154 or with CD 154 expressed on the membrane of leukocytes ( CD 154 transfected J 558 cells ) or of activated platelets , stimulated the synthesis of PAF by endothelial cells but not by smooth cells . ^^^ The synthesis of PAF triggered by activated platelets was inhibited by a soluble CD 40 murine Ig fusion protein that prevents the interaction between membrane CD 40 and CD 154 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : CD 40 is a member of the tumor necrosis factor ( TNF ) family of receptors , whose ligand , CD 154 , is expressed by activated mononuclear cells . ^^^
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The CD 40 molecule has an important role in promoting inflammatory responses by macrophages / microglia , since interaction with its cognate ligand , CD 154 , leads to secretion of cytokines and neurotoxins . ^^^
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In the unstimulated condition , CD 40 was associated with Cav 1 , and with activation of the receptor by its cognate ligand CD 154 , CD 40 disassociated from Cav 1 . ^^^
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These results indicated that activation of AMphi through CD 40 might be involved not only in amplification by the interaction with CD 154 but also in the development of ALI by CD 40 itself , and that the functional blockade of CD 40 would yield one of the targets for the treatment of LPS induced ALI and acute respiratory distress syndrome . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blockade of CD 40 CD154 costimulatory pathway in mice and primates with anti CD 154 monoclonal antibodies results in prolonged survival of vascularized organs and islet grafts . ^^^ CD40Ig , a recombinant fusion protein comprised of the extracellular domain of human CD 40 molecule in frame fused with the site mutated human IgG 1 Fc region , abrogated the cognate interaction of CD 40 CD154 pathway by binding the CD 154 molecule . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T B cell interactions were assessed by means of blocking antibodies to CD 154 , CD 40 , CD 69 , and CD 18 ; these interactions were not specially affected by infliximab treatment . ^^^
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RESULTS : ABI 793 binds to human , Cynomolgus and Rhesus monkey CD 154 ; it inhibits dose dependently in vitro CD 154 : CD 40 binding and human mixed lymphocyte reaction . ^^^
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Development of the acquired immune response is dependent on the signaling of CD 40 by its ligand , CD 154 . ^^^
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Endothelial and NK cell priming , with LPL and HS+LPL , significantly induced CD 40 and CD 154 expression , respectively . ^^^ Treating endothelial cells simultaneously with indomethacin , CD 154 fusion protein , and Wortmanin prevented the CD 40 effect increasing xenograft rejection . ^^^ LPL and HS+LPL protect bovine endothelial cells from NK cytotoxicity by inducing CD 40 , CD 154 expression , and secretion of soluble factors . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
APC activation is fundamentally associated with binding of CD 40 to its ligand CD 154 . ^^^
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In vitro characterization demonstrated that tgD and tgD CD 154 both bind to cultured bovine B cells , whereas only tgD CD 154 induces interleukin 4 dependent proliferation , suggesting that tgD CD 154 specifically binds the CD 40 receptor and induces receptor signalling . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cognate recognition between the CD 40 receptor and its ligand , CD 154 , is thought to play a central role in the initiation and propagation of immune responses . ^^^
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CD 40 , a member of the nerve growth factor / tumor necrosis factor receptor superfamily , and its ligand , CD 154 , play essential roles in cell immune responses . ^^^ Ligation of CD 40 on cancer cells was also found to produce a direct growth inhibitory effect through cell cycle blockage and / or apoptosis with no overt side effects on normal cells and treatment with CD 154 can heighten tumor rejection immune response as well . ^^^ This review focuses on the latest discovered functions of CD 40 CD154 interaction in cell immune responses and on the new findings of CD 154 based human cancer therapy . . ^^^
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This study provides evidence that CD 40 transduced signals initiate T ( R ) cell increase in vivo and that CD 154 transduced signals sensitize autoaggressive CD 8 ( + ) T cells to suppression . . ^^^
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Additional changes in the activation state of PBMCs , upregulation of the costimulatory molecules CD 40 and CD 80 , of the accessory molecule CD 154 , and of interferon gamma expression were identified only in AA patients where the disease was advancing , i . e . these changes were independent of the extent of hair loss and were not seen in patients with spontaneous or DPCP treatment induced , regressing AA . ^^^
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RNAi mediated silencing of CD 40 prevents leukocyte adhesion on CD 154 activated endothelial cells . ^^^
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Ligation of CD 40 by CD 4 T cells through CD 154 is key both to germinal centre induction and follicular T dependent Ig class switching , but its requirement for aspects of T cell priming and extrafollicular antibody responses is less clear . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 on Ramos B cells by recombinant CD 154 caused TRAF 2 and TRAF 3 to dissociate , whereas overexpression of TRAF 3 in Ramos B cells inhibited CD 154 induced TRAF 2 mediated activation of NF kappaB . ^^^
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Our hypothesis was that blocking the interaction between CD 154 ( CD40L ) on Th cells and CD 40 on antigen presenting cells should inhibit T cell activation , and thus the development of EAG . ^^^
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Cd 40 but not CD 154 knockout mice have reduced inflammatory response in polymicrobial sepsis : a potential role for Escherichia coli heat shock protein 70 in CD 40 mediated inflammation in vivo . ^^^ However , recent data suggests that CD 40 maybe activated by CD 154 or directly via bacterial heat shock protein ( HSP ) 70 . ^^^ Wild type ( WT ) , CD 40 , and CD 154 underwent cecal ligation and puncture ( CLP ) . ^^^ The differential response of CD 40 and CD 154 mice to CLP was not due to a general attenuated response to inflammatory stimuli , as all three strains had similar survival after LPS administration , and CD 40 macrophages had normal production of IL 12 in response to lipopolysaccharide . ^^^ This data demonstrates CD 154 independent CD 40 activation in polymicrobial sepsis and suggests that bacterial HSP 70 is capable of stimulating CD 40 in vitro and in vivo . . ^^^
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The list of such targets includes the ligand pairs CD 28 : B 7 , CD 154 : CD 40 , LFA 1 : ICAM 1 , ICOS : B7RP 1 , and VLA 4 : VCAM 1 . ^^^
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The stimulation of CD 40 positive cervical carcinoma cell lines with soluble CD40L ( CD 154 ) resulted in activation of the NF kappaB and MAPK signaling pathways and up regulation of cell surface markers and intracellular molecules associated with Ag processing and presentation . ^^^ Concomitantly , the CD 154 induced activation of CD 40 in carcinoma cells was found to directly influence susceptibility to CTL mediated killing . ^^^
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To circumvent this potential complication , we developed and evaluated a chimeric Ab targeting CD 40 ( Chi 220 , BMS 224819 ) as an alternative to CD 154 . ^^^
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The expression of CD 154 is very tightly regulated by the immune system through a number of non redundant overlapping mechanisms that ensure its limited initial induction , along with its temporal maintenance and rapid elimination from the cell surface , and its functional neutralization by the release of soluble CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 and CD 154 belong to the tumor necrosis factor ( TNF ) receptor superfamily and the TNF superfamily , respectively . ^^^
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We demonstrate that IL 7 mediated the up regulation of CD 25 , CD 95 and human leucocyte antigen DR , while it did not alter the expression of CD45RO , CD 69 , CD 40 , or CD 154 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The aim of the present study was to evaluate the role of recipient CD 40 and CD 154 in the rejection process of concordant and discordant islet xenotransplantation ( Tx ) . ^^^ METHODS : Diabetic C57BL / 6 mice , CD 40 or CD 154 knockout ( KO ) mice were transplanted with either concordant rat or discordant human islets . ^^^ Experimental design : group 1 , control ( ie , C57BL / 6 mice received islet Tx without therapy ) ; group 2 , C57BL / 6 mice received islet Tx with anti CD 154 monoclonal Ab ( mAb ) therapy ; group 3 , CD 40 KO mice ; and group 4 , CD 154 KO mice were used as recipients without therapy . ^^^ In CD 40 KO and CD 154 KO recipients , survival of concordant or discordant islets was not prolonged significantly compared to control groups . ^^^ Rejection of xenografts and strong MLR responses against donor cells in vitro in CD 40 or CD 154 KO animals is possible through efficient activation of alternate pathways of costimulation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The aim of the study was to evaluate the role of recipient CD 40 and CD 154 in the rejection process of concordant and discordant islets xenotransplantation ( Tx ) . ^^^ Diabetic C57BL / 6 mice , CD 40 or CD 154 knockout ( KO ) and complement C 3 deficient ( C 3 / ) mice were transplanted with either rat or human islets . ^^^ Group 1 , C57BL / 6 Tx without therapy ; Group 2 , C57BL / 6 Tx with anti CD 154 monoclonal antibody ( mAb ) ( MR 1 ) therapy ; Group 3 , CD 40 KO ; and Group 4 , CD 154 KO Tx without therapy ; Group 5 , C 3 / Tx without therapy and Group 6 , C 3 / Tx with MR 1 therapy . ^^^ In Groups 3 , 4 and 6 , MLR responses were not modified by the absence of CD 40 or CD 154 molecules , or MR 1 and were similar to Group 1 . ^^^
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We therefore conclude that CLL cell cytoprotection in the presence of CD 154 requires combined signalling by both CD 40 and VEGFR . ^^^ In conclusion , our findings identify autocrine VEGF as an important mediator of the antiapoptotic effect of CD 40 ligation , and thus provide new insights into CLL cell rescue by CD 154 in lymphoreticular tissues . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The aim of the present study was to investigate on cultured human mesangial cells ( HMC ) whether oxLDL induces apoptosis by a mechanism dependent on the inhibition of Akt survival pathway , and whether the engagement of mesangial CD 40 by its ligand CD 154 inhibits the apoptotic effect of oxLDL . ^^^ Indeed , CD 154 induced a rapid enhancement in Akt enzymatic activity , that was temporarily correlated with the association of CD 40 with TRAF 3 , TRAF 6 , c Cbl and the p 85 subunit of PI3K . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Their involvement in the pathogenesis of chronic inflammatory bowel disease ( IBD ) has been revealed by increased expression of CD 40 and CD 154 in the inflamed mucosa of patients and the therapeutic effects of anti CD 154 antibodies in experimental colitis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : The ligation of CD 40 by CD 154 is a critical step in the interaction between APC and T cells . ^^^
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We analyzed 72 formalin fixed and paraffin embedded sequential heart allograft biopsies from 22 patients by quantitative real time reverse transcriptase polymerase chain reaction for the mRNA expression of the costimulatory molecules CD 28 , CD 80 , CD 86 , CD 40 , and CD 154 . mRNA expression levels were correlated to histological grade and time point of rejection . mRNA expression of costimulatory molecules predominantly expressed by antigen presenting cells ( CD 80 , CD 86 , CD 40 ) , correlated with histological grade of cell mediated acute rejection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When expressed at the platelet surface and exposed to CD 40 expressing vascular cells , the platelet associated CD 154 triggers a variety of pro inflammatory and pro coagulant responses including induction of adhesion receptors , release of cytokines and chemokines , induction of tissue factor and of metalloproteinases . ^^^ Recent data show that CD 40 bearing cells involved in fibrosis such as hepatic stellate cells and glomerular mesangial cells also respond to platelet associated CD 154 , thus suggesting a new mechanism by which platelets may be instrumental in the inflammatory diseases of the liver or the kidney . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Activation of mesangial cells by platelets in systemic lupus erythematosus via a CD 154 dependent induction of CD 40 . ^^^
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Nave and memory B lymphocyte populations are activated by CD 154 interaction through cell surface CD 40 . ^^^ Our results show that the level of CD 154 binding to CD 40 on B lymphocytes can directly influence the evolution of CD 19 ( + ) CD 27 ( ) and CD 19 ( + ) CD 27 ( + ) cell populations . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 154 CD 40 interactions drive hepatocyte apoptosis in murine fulminant hepatitis . ^^^ Moreover , fulminant hepatitis of various etiologies is characterized by a hepatic influx of CD 154 expressing T cells and an upregulation of CD 40 expression on Kupffer cells and hepatocytes , implicating this pathway in the pathogenesis of fulminant hepatitis . ^^^ In this study , we used a murine model of fulminant hepatitis induced by concanavalin A ( con A ) and documented a significant influx of CD 154 expressing T cells into the livers of mice treated with con A , in association with markedly increased expression of CD 40 restricted mainly to hepatocytes in damaged areas of the liver . ^^^ In conclusion , CD 154 stimulation of CD 40 plays a central role in hepatocyte death in fulminant hepatitis through direct and indirect pathways that may have direct therapeutic implications in humans . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 , a member of the TNF receptor superfamily , mediates intercellular communication between fibroblasts and cells that express CD 154 ( CD40L ) , including T lymphocytes and platelets . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Engagement of CD 40 on murine B cells by its ligand CD 154 induces the binding of TNFR associated factors ( TRAFs ) 1 , 2 , 3 , and 6 , followed by the rapid degradation of TRAFs 2 and 3 . ^^^
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FIV infected CD8+ T cells showed markedly increased CD 154 expression ( p < 0 . 05 ) , whereas neurons were the predominant cells expressing CD 40 in DRGs . ^^^
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Our laboratory has shown that the interaction of B and CD 4 T cells that is central to MAIDS pathogenesis requires ligation of CD 154 on CD 4 T cells with CD 40 on B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This defect is probably due to impaired CD 40 dependent IL 12 production in vivo , because administration of anti CD 40 Ab , or rIL 12 , restored IFN gamma production by sdLN cells from CD 154 / mice . ^^^ CD 154 ligation of CD 40 was not required for the migration of skin derived APCs , but did have a limited role in their maturation ( increased MHC 2 and CD 86 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functional interactions between B and T lymphocytes are known to depend on the expression of co stimulatory molecules B7 . 1 / CD80 , B7 . 2 / CD86 and their counter receptors CD 28 and CTLA 4 , as well as CD 40 and its ligand CD40L . ^^^
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The finding that Src expression is inducible and correlates with stimulation by CD 154 and the proliferation of the B cells suggests that Src may play a specific role in normal and transformed B cell activation / proliferation pathways mediated primarily through CD 40 stimulation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The signaling of CD 40 by its ligand , CD 154 , between T and B cells is required for humoral immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The aim of the study was estimation of a concentration soluble form of CD 40 , CD40L ( CD 154 ) in the bacterial and viral inflammation of the central nervous system in two compartments blood circulation and intrathecally , before and after the treatment . ^^^
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While blockade of endogenous CD 154 resulted in a remarkable increase in parasite load in IFN gamma / mice infected with Toxoplasma gondii , in vivo administration of a stimulatory anti CD 40 monoclonal antibody markedly reduced parasite load . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 154 is a cell surface molecule expressed on activated T cells that binds to CD 40 , an activating molecule on APCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction between B cell CD 40 and its ligand ( CD40L ) on activated Th cells provides a critical signal necessary for T cell dependent isotype switching . ^^^ To assess the effects of the CD40L CD 40 interaction on germ line Ig transcript expression in murine B cells , a membrane bound form of mouse CD40L was expressed in the baculovirus system . ^^^ These results demonstrate that the CD40L CD 40 interaction and IL 4 activate germ line Ig gene transcription by distinct but synergistic mechanisms and suggest that multiple signals may be required to induce sufficient germ line transcription and / or germ line transcript levels necessary to target switch recombination . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we describe the results of experiments which demonstrate that the B cells from these animals respond abnormally to ligation of CD 40 , a B cell surface molecule now known to play a key role during T cell B cell interactions , via its interaction with the counterligand ( CD40L ) expressed by activated T cells . ^^^ Hence , xid B cells fail to proliferate when cultured with preactivated T helper type 2 ( Th 2 ) T cells ( known to express CD40L ) , with a soluble CD40L CD 8 fusion protein , or in response to monoclonal antibodies to CD 40 , even in the presence of IL 4 and / or anti Ig reagents . ^^^
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Engagement of CD 40 by its soluble ligand ( CD40L ) enhanced both clonogenic capacity and colony cell survival of HD cell lines . ^^^
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Because the ligand of CD 40 ( CD40L ) is expressed on activated T helper cells , B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens , which are normally able to activate T helper cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L is the predominant protein induced on activated T cells which constitutes contact dependent help , and lack of CD40L or blocking CD 40 CD40L interactions leads to severely impaired antibody production . ^^^ In addition to CD 40 CD40L , B and T cells express costimulatory , accessory molecules which amplify T and B cell function and allow for reciprocal dialogues during antigen presentation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
B lymphocyte antigen CD 40 and its ligand , CD40L ] . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To address elements that might uniquely characterize CD 40 mediated signaling , the nuclear expression of three transcription factors was evaluated following B cell stimulation by CD40L and by anti Ig antibody . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
LX , and freshly isolated resting splenic B cells were cultured with either soluble or membrane bound forms of the T cell ligand for CD 40 ( CD40L ) , in the presence or absence of additional signals provided by Ag or anti IgM , interleukin 4 , and class 2 specific monoclonal antibody ( mAb ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L , a membrane protein of activated T cells , interacts with the B cell receptor CD 40 . ^^^ In this report , we have demonstrated that the signal mediated by CD40L acts upon bcl 10 , a bcl 2 homologue . bcl 10 expression is strongly enhanced by CD 40 receptor engagement , while there is little or no induction by sIg cross linking . ^^^ This result suggests that the up regulation of bcl 10 by CD40L plays an important role in CD 40 mediated apoptotic rescue in murine B cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we report that the signal for abrogating sIg mediated apoptosis is generated by association of the CD40L molecule on T cells with the CD 40 molecule on WEHI 231 cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The human cell lines HMC 1 ( mast ) and KU 812 ( basophilic ) both express the ligand for CD 40 ( CD40L ) which is shown to be responsible for the IgE production . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L on activated T cells from HIGM 1 patients fails to bind B cell CD 40 molecules , and subsequent analysis of CD40L transcripts by reverse transcription PCR demonstrated coding region mutations in these patients . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expression of the ligand for CD 40 ( CD40L ) is critical for induction of T cell dependent Ab responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Diagnostic significance of CD 40 , CD40L , and CD 26 expression in Hodgkin ' s disease and other lymphomas . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the present experiments , IL 12 is shown to be produced by murine spleen antigen presenting cells ( APC ) by interaction with T helper 1 ( Th 1 ) clones through CD 40 CD40 ligand ( CD40L ) interaction , but not with Th 2 clones . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate the role of these receptor structures in the pulmonary environment , we evaluated the expression of some members of the TNF receptor ( CD 27 , CD 30 , CD 40 , CD95 / Fas , CD120a , and CD120b ) and TNF ligand ( CD40L , CD70 / CD27L , CD30L , and mTNFalpha ) superfamilies by bronchoalveolar lavage ( BAL ) T cells recovered from healthy subjects and patients with interstitial lung disease ( ILD ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Concentrations of CD40L that saturated 40 to 100 % of CD 40 induced initial homotypic aggregation followed by Fas ( CD 95 ) independent apoptosis , with resultant decreases in growth and Ig secretion . ^^^ Concentrations of CD40L that saturated 15 to 25 % of CD 40 also stimulated aggregation of all hybridomas . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Normal B lymphocyte antibody production requires T cell costimulatory signals provided in part by T cell surface expression of gp39 / CD40ligand ( CD40L ) that engages the B cell receptor CD 40 and induces B cell differentiation and immunoglobulin class switching . ^^^ We assessed the effect of disrupting the CD40L CD 40 costimulatory pathway , using a CD 40 Ig fusion protein , on the development of membranous glomerulonephritis ( MGN ) in the mouse . ^^^ These observations provide evidence that disruption of the CD 40 CD40L costimulatory pathway can prevent the development of MGN by suppressing T cell dependent antibody production . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Suppression of the response was observed with administration of neutralizing Abs specific for either CD 40 or CD40L , or with the soluble fusion protein human CD 40 Fc . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40 ligand ( CD40L ) interaction is required for the generation of antibody responses to T dependent antigens as well as for the development of germinal centers and memory B cells . ^^^ The role of the CD 40 CD40L interaction in the induction of antigen specific . ^^^ Using CD 40 and CD40L deficient mice together with lymphocytic choriomeningitis virus and vesicular stomatitis virus as viral model antigens , this study corroborates earlier findings that no lg isotype switching of virus specific antibodies was measurable upon infection of CD 40 or CD40L deficient mice . ^^^ In contrast , in vivo induction of virus specific CD4+ T cells measured by proliferation and cytokine secretion of primed virus specific Th cells in vitro was not crucially dependent on the CD 40 CD40L interaction . ^^^ Th mediated effector functions distinct from and in addition to T B collaboration were analyzed in CD 40 and CD40L deficient and normal mice : ( a ) local inflammatory reactions upon LCMV infection mediated by LCMV specific Th cells were not dependent on a functional CD 40 CD40L interaction , ( b ) cytokine mediated protection by CD4+ T cells primed by vesicular stomatitis virus against a challenge infection with recombinant vaccinia virus expressing the glycoprotein of vesicular stomatitis virus was found to be equivalent in CD40L deficient and normal mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interaction between the CD 40 molecule expressed by BRG M cells and its specific ligand ( CD40L ) prevented p 45 induced cell apoptosis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Over the past three years , CD 40 and its ligand ( gp 39 , CD40L , TBAM ) have been shown to be essential for humoral immune responses to thymus dependent antigens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We found that ligation of CD 40 by CD40L triggers the production of extremely high levels of bioactive IL 12 . ^^^ These findings reveal a new role for CD 40 CD40L interaction in regulating DC function and are relevant to design therapeutic strategies using cultured DCs . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L was normally expressed by activated T cells , but CD 40 mediated signal transduction was defective in B cells , preventing heavy chain switching ( CD40L+ type of the HIM syndrome ) . ^^^ Accordingly , the blockade of CD40L CD 40 interactions , which has been proposed as a treatment of RA , might not be adapted to all patients . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The ligand for CD 40 ( CD40L ) is expressed on the surface of activated CD4+ T cells and its role in T B cell collaborations and thymus dependent humoral immunity is well established . ^^^ Together , the results show that CD 40 CD40L interactions are required for strong antiviral humoral immune responses , and reveal a novel role for CD40L in the establishment and / or maintenance of CD8+ CTL memory . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40L interactions between resting B cells and activated T cells are essential for germinal center formation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The natural ligand of CD 40 ( CD40L , gp 39 , or T BAM , for T B cell activating molecule ) was recently identified as an inducible molecule expressed transitionally on activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent data have shown that CD 40 antigen and its ligand ( CD40L ) , and the CD 26 molecule are differentially expressed among Hodgkin ' s disease ( HD ) ( CD40+ , CD 26 , CD40L ) , peripheral T cell ( PTCL ) ( CD40L+ / , CD 40 , CD 26 ) and CD 30 positive anaplastic large cell ( ALC ) ( CD26+ , CD40L ) lymphomas . ^^^ Finally , the combined application of anti CD 40 and anti CD40L MoAbs provides additional significant features in differentiating HD from PTCL due to the constant expression of CD40+ / CD40L phenotype in HD and its absence in PTCL , which in turn express CD40 / CD40L+ / phenotype . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD 40 CD40L interactions in Th 1 T cell responses . ^^^ On the other hand , the administration of anti gp 39 antibody did not influence Th 2 T cells responses , suggesting that CD 40 CD40L interactions are more crucial for Th 1 than Th 2 T cell development . ^^^ In addition , stimulation of CD 40 on human monocytes results in IL 12 production , suggesting that activated T cells expressing CD40L may directly induce the production of IL 12 by antigen presenting cells , thus allowing for the generation of a Th 1 T cell response in the absence of intracellular pathogens . ^^^ We investigated whether the CD 40 CD40L interaction was important in the production of IL 12 by DCs in an in vitro system that allowed precise control of cytokine concentrations . ^^^ Initially we showed that FACS purified mouse spleen DCs produce high amounts of IL 12 p 40 in response to CD 40 crosslinking by CD40L expressing fibroblasts . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The results define a 95 bp CD 30 responsive element ( CD30RE ) located 5 ' of the previously defined CD 40 responsive element ( CD40RE ) that resides within the same 95 bp fragment as the IL 4RE and ablates CD40L induced 1 epsilon promoter activity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Functions of CD 40 and its ligand , gp 39 ( CD40L ) . ^^^ Initially , a role for the interaction between CD 40 , expressed on B cells , and gp 39 ( CD40L ) , expressed on activated T cells , has been defined in humoral immunity . ^^^ CD 40 CD40L interaction is an essential signal for B cell proliferation , expression of activation markers , immunoglobulin production , and isotype switching . ^^^ CD 40 CD40L interaction is also required for formation of B memory cells and germinal centers , and signaling through CD 40 prevents apoptosis of germinal center B cells . ^^^ More recent evidence indicates an expansion of the role of the CD 40 CD40L axis in cellular interactions beyond antibody formation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This B cell fate decision is determined by the concerted action of two surface proteins on activated T cells , CD 40 and Fas ligands ( CD40L and FasL ) , whose effects are switched by signals from the B cell antigen receptor ( BCR ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IL 12 production was dependent on the presence of CD4+ , but not of CD8+ , T cells , and inhibited by addition of anti CD 40 ligand ( CD40L ) mAb . ^^^ However , the B cell depleted fraction from CD 40 / mice failed to produce IL 12 when stimulated with anti CD 3 or Con A or when cocultured with CD40L expressing Chinese hamster ovary cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate the potential role of the CD40 / CD40 ligand ( CD40L ) pathway in this process , we first determined the effects of blocking anti CD 40 or anti CD40L monoclonal antibodies ( mAb ) on the development of monocyte PCA during mixed lymphocyte reaction ( MLR ) between allogeneic peripheral blood mononuclear cells ( PBMC ) . ^^^ The strong inhibitory effect exerted by both mAb ( mean percentages of inhibition : 88 and 91 % for anti CD 40 and anti CD40L mAb , respectively ) indicates that CD40 / CD40L interactions are required for the induction of PCA in MLR . ^^^ These data led us to measure monocyte PCA after incubation of PBMC or purified monocytes with a stimulating anti CD 40 mAb ( BL C 4 ) or with 3T6 fibroblasts transfected with the gene encoding CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
There are two pathways of IL 12 induction in phagocytic cells : a T cell independent one , induced primarily by bacteria , bacterial products , or intracellular parasites and important in the early inflammatory response of innate resistance ; and a T cell dependent one , induced by the interaction of CD40L on activated T cells with CD 40 receptor on IL 12 producing cells ( phagocytic cells and antigen presenting cells ) and important in the regulation of adaptive immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
HIGM 1 results from a partial failure of the interaction between T helper cells and B cells owing to mutation of the ligand ( CD40L ) expressed on T cells which normally interacts with B cell receptor CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunohistologic analysis of renal CD 40 and CD40L expression in lupus nephritis and other glomerulonephritides . ^^^ METHODS : Renal in situ CD40L and CD 40 expression was examined in patient biopsy specimens . ^^^ Immunohistochemical studies were performed on frozen sections utilizing anti CD40L monoclonal antibody ( MAb ) , anti CD 40 MAb , or control MAb . ^^^ However , these findings were not unique to lupus GN : up regulation of CD 40 and CD40L expression was similarly observed in other inflammatory renal diseases . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40L interactions provide `` third party ' ' costimulation for T cell response against B 7 1 transfected human breast tumor cells . ^^^ In this study we provide evidence that a human breast carcinoma cell line , MDA MB 231 ( MDA ) , can be made immunogenic following B 7 transfection and that full T cell activation is obtained through cooperation of T B lymphocytes via CD 40 CD40L interactions . ^^^ These data suggest that the engagement of CD40L on T cells with CD 40 on B cells provides a costimulatory signal which , in synergism with TCR dependent MDAB 7 T cell recognition ( signal 1 ) and B7 / CD28 interactions ( signal 2 ) , leads to full T cell activation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The induction of IL 4 synthesis by Ag specific B cells was significantly reduced by blocking CD 40 CD40 ligand ( CD40L ) interactions or by the addition of small quantities of rIL 12 . ^^^ These results suggest that B cells activated by their cognate Ag preferentially induce IL 4 synthesis as a result of the interaction of CD40L on T cells with CD 40 , whereas SAC preferentially induce IFN gamma synthesis by T cells as a result of their greater production of IL 12 and their limited capacity to trigger CD40L on T cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Significant decreases were observed after VIT for proliferative response to venom and venom + IL 4 , IL 4 secretion , Fc epsilon RII , CD 40 , and CD40L expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 is a 48 kDa glycoprotein predominantly expressed on B cells in both mouse and man , which interacts with a counterligand ( CD40L ) , expressed on activated CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
RESULTS : By immunostaining and mRNA analysis , we were able to show that eosinophils from normal donors and patients with HD , HES , and HE express a number of receptors and ligands of the TNF superfamily , including CD 40 , CD40L , CD30L , CD95 / Fas , CD95 / FasL and 4 1BB . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Our data indicate that CD 40 ligation does not directly lead to GC formation but it plays an indirect role related to maturation of helper T cells ; signalling is bidirectional , to B cells , via CD 40 , upregulating cytokine receptor expression and to T cells , via CD40L , causing secretion of cytokines necessary for GC initiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS AND RESULTS : Stimulation of human monocytes / macrophages through CD 40 by either membranes from activated T cells or recombinant CD40L ( rCD40L ) induced expression of interstitial collagenase , stromelysin , and TF protein and activity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expression of HLA DR , CD 40 , CD40L , CD 80 , and CD 28 was investigated on thyroid samples from 30 patients ( Graves ' disease , n = 16 ; benign toxic adenoma , n = 8 ; multinodular goiter , n = 6 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The authors studied CD 40 ligant ( CD40L ) expression and interleukin 10 ( IL 10 ) production in 16 patients with common variable immunodeficiency ( CVI ) . ^^^
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Although it is well established that CD 40 and its ligand ( CD40L ) play pivotal roles in the development of humoral immunity , their roles in cell mediated immunity and cell mediated autoimmune diseases are not well defined . ^^^ We report here that CD 40 : CD40L interaction is crucial for the development of experimental autoimmune encephalomyelitis ( EAE ) , a prototype TH 1 cell mediated autoimmune disease . ^^^ These results suggest that CD 40 : CD40L interaction plays important roles in the differentiation of autoreactive TH 1 versus TH 2 cells in vivo , and that CD40L blockade is effective in preventing autoimmune encephalomyelitis . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
With the use of L cells stably transfected with both CD 32 and CD40L , anti Ig beta activation of B cells was combined with CD 40 triggering , an important component of the T cell dependent B cell activation . ^^^
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Surprisingly , not only the TNFR 1 , but also the TNFR 2 , was able to mediate the antiviral effects of TNF alpha in vivo , whilst the antiviral activity observed following CD 40 CD40L interaction is a newly defined function which may involve apoptosis of infected cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 on antigen presenting cells by its cognate ligand CD40L is thought to induce expression of CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) . ^^^ CONCLUSIONS : The CD 40 CD40L pathway appears important in regulation of allogeneic DC T cell functional interaction in vivo ; its blockade increases markedly the potential of costimulatory molecule deficient DCs of donor origin to induce long lasting allograft survival . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 and its ligand ( gp 39 , CD40L , TBAM ) is central to the control of thymus dependent humoral immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Special attention was directed to the roles of Fas ( CD 95 ) Fas ligand ( FasL ) interactions and CD 40 CD40 ligand ( CD40L ) interactions . ^^^ These results indicate that signals achieved by direct interactions through CD 40 CD40L exert bidirectional effects on the outcome of humoral immune responses depending on the state of activation of B cells and on the extent of CD 40 ligation . ^^^ Moreover , the data suggest that CD 40 CD40L interactions rather than Fas FasL interactions may play more critical roles in direct cellular collaboration between B cells and anti CD 3 stimulated CD4+ T cells to prevent the extension of responses of inappropriately activated B cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interleukin 12 is produced in response to infection with bacteria or parasites or to bacterial constituents such as LPS in monocytes / macrophages and dendritic cells , and also generated by the interaction between activated T cells and antigen presenting cells via CD 40 CD40 ligand ( CD40L ) . ^^^ These cells , stimulated by an agonistic monoclonal antibody against CD 40 or by transfection with a CD40L expression vector , secreted p 40 and showed enhanced p 40 mRNA expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This signal is delivered by lymphocytes T , but these cells can be replaced by Epstein Barr virus infection , protein gp 39 ( CD40L ) , monoclonal antibodies to CD 40 and CD 58 , membrane TNF alpha , as well as corticosteroids . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In particular , CD 40 was commonly observed on all B cells ( both tumor and normal ) , whereas its ligand ( CD40L ) , which is usually undetectable on resting normal B lymphocytes , was expressed in CLL and HCL but not in other chronic lymphoproliferative disorders . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expansion or elimination of a B cell clone is determined through a complex interaction of the tumour necrosis factor receptor / nerve growth factor receptor family members CD 40 and Fas , which are expressed on the B cell surface , with their respective physiological ligands ( CD40L and FasL ) expressed on the surface of CD4+ T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 28 independent GVHD was significantly improved by treatment with anti CD 40 ligand ( CD40L ) mAb . ^^^ These data suggested that the CD40L CD 40 pathway significantly contributed to the CD 28 independent pathogenesis of acute GVHD , whereas the CTLA 4 B7 pathway acted protectively in the development of GVHD . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The specificity of this finding was established by inhibition experiments using monoclonal antibodies ( mAbs ) blocking CD 40 or CD40L . ^^^ To investigate the role of CD40 / CD40L interactions in the induction of endothelial cell PCA by lymphocytes , interferon ( IFN ) gamma stimulated EC were incubated with T cells in the absence or presence of anti CD 40 or anti CD40L mAb . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The co localization of CD4+ T cells and macrophages in the lesion , the abundant expression of HLA Class 2 molecules and the co stimulatory molecule CD 40 and its ligand ( CD40L ) indicate a contribution of cell mediated immunity to atherogenesis . ^^^
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In the present study , we report that such release can be achieved purely with soluble reagents whereby co operative epitopes on CD 40 are targeted by the combined use of mAb and soluble recombinant CD40L . ^^^
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The expression of CD 40 and CD40L in the CNS was increased during clinical attacks . ^^^
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Since the function and the fate of the immune system cells are governed principally by the biochemical events that follow ligation of specialized cell surface receptors , we will review in this article recent developments in our understanding of abnormalities in the biochemistry of signals generated either by the antigen receptor complex or by systems of costimulatory cell surface molecules , like the CD28 / CTLA4 : CD80 / CD86 and the CD 40 : CD40L pairs found on the surface membrane of lupus immune cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L CD 40 interactions regulate endothelial cell surface tissue factor and thrombomodulin expression . ^^^ CD40L is an activation induced CD4+ T cell surface molecule that functionally interacts with CD 40 expressed on endothelial cells . ^^^ In this study we ask if CD40L CD 40 interactions modulate endothelial cell surface tissue factor or thrombomodulin expression in vitro . ^^^ Together these data suggest that CD40L CD 40 interactions may directly regulate endothelial cell procoagulant activity during inflammatory responses . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This was not related to binding of CTLA4Ig or CD40L fusion proteins , implying similar levels of expression of their ligands , CD 80 and CD 86 or CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : CTLA 4 / CD28 B 7 and CD 40 CD40L interactions constitute two key costimulatory pathways in lymphocyte signalling during experimental allograft rejection . ^^^ Expression of B 7 1 and B 7 2 and their counter receptor CTLA 4 , and of CD 40 and its counter receptor CD40L was examined . ^^^ In contrast , CD 40 and CD40L were rarely expressed . ^^^ In patients with cyclosporin nephrotoxicity cells positive for B 7 1 , B 7 2 , CTLA 4 , CD 40 , or CD40L were absent . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have recently found that Abeta induces expression of adhesion molecules , and that cytokine production and interaction of CD 40 CD40 ligand ( CD40L ) further increase the Abeta induced expression of adhesion molecules in these same cells . ^^^ These results suggest that Abeta can function as an inflammatory stimulator to activate vascular cells and induces an auto amplified inflammatory molecular cascade , through interactions among adhesion molecules , CD 40 CD40L and cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When T cells were activated , both T and B cells from the transgenic and control mice expressed an equivalent amount of CD40L and CD 40 , which are , respectively , the receptor and counter receptor for the interaction between T and B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The interactions of CD 40 and CD40L have been known for some time to critically regulate B cell responses with respect to proliferation , isotype switching , antibody production , and memory formation . ^^^ Here , currently available data are reviewed demonstrating that CD 40 CD40L interactions are operational in two chronic inflammatory clinical conditions , namely , multiple sclerosis and atherosclerosis . ^^^ The functional correlates of these interactions are discussed in the light of recent other findings , shedding light on the multiple effects of CD 40 CD40L interactions . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we show that anti CD 40 ligand ( CD40L ) mAb does not prevent the expression of B 7 2 on allogeneic rB cells in vivo but did prolong donor specific cardiac allograft survival . ^^^ Moreover , pretreatment with professional APCs combined with anti CD40L mAb induced hyporesponsiveness to alloantigens in vivo . rB cells from CD 40 knockout mice were unable to induce unresponsiveness , while graft prolongation was achieved in CD40L knockout recipients pretreated with wild type rB cells . ^^^ These data suggest that CD 40 CD40L interactions in the recipient play a critical role in the induction of hyporesponsiveness to alloantigens in vivo and that the effect of the CD 40 pathway may be independent of its effect on the B 7 costimulatory pathway . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Rather , the stimulatory T APC weakness was due to their inability , because they are CD 40 , to provide the appropriate co stimuli to responder T cells both indirectly via IL 12 , and partially via direct CD40L triggering on T cells . ^^^
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The provision of excess costimulation can overcome some of the defects in T cell activation by weak agonists , but is insufficient to induce a sufficient level of CD40L expression needed for engagement of CD 40 on APC with subsequent IL 12 production and induction of IL 12Rbeta2 chain expression . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The multimeric CD 40 bound with high affinity to stably transfected mouse fibroblasts expressing CD40L . ^^^
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BCL 6 ( ) / syn 1 ( + ) tumor cells of CHD are mostly found surrounded by T cells expressing CD40L , consistent with the observation that CD 40 signaling downregulates BCL 6 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent progress in the understanding of immune function indicates that the interaction of CD40L with its receptor , CD 40 , plays a pivotal role in both humoral immunity and cell mediated defense against pathogens . ^^^ Several reports have shown that soluble forms of CD40L , like CD 40 antibodies , are biologically active . ^^^
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These results demonstrated that activation of ECs by interaction with T cells induced synthesis and release of MMPs and promoted an angiogenic function of ECs via CD40L CD 40 signaling . ^^^
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The interaction of CD 40 on antigen presenting cells ( APC ) with CD40L on mouse thyroglobulin ( MTg ) specific T cells may deliver an essential signal for the development of CD 4 ( + ) experimental autoimmune thyroiditis ( EAT ) effector cells and anti MTg producing B cells . ^^^ To determine the requirement for CD 40 CD40L interactions in G EAT , donor mice were injected with an anti CD40L monoclonal antibody ( mAb ) on days 1 , 0 , and +1 relative to immunization with MTg and adjuvant . ^^^ Addition of anti CD40L during in vitro activation of MTg primed spleen cells or treatment of recipients with anti CD40L had no effect on EAT severity , indicating that CD 40 CD40L interactions are not required after EAT effector cells are primed to MTg . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The stimulation of B CLL cells via CD 40 by culture on CD40L expressing feeder cells induced a strong upregulation of costimulatory and adhesion molecules and turned the B CLL cells into efficient antigen presenting cells ( APCs ) . ^^^ In marked contrast , autologous CD 40 CLL cells did not induce a relevant CTL response , but rather stimulated a CD 4 ( + ) , Th 1 like T cell population that expressed high levels of CD40L and released interferon gamma in response to stimulation by CD 40 CLL cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 upregulation in TCR alpha / beta+ CD68+ cells and parenchymal CD40L induction and associated with NF kappa B activation in chronic rejecting human renal allografts . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Because of the critical role of the CD 40 CD40 ligand ( CD40L ) pathway in the induction and effector phases of immune responses , we investigated the effects of CD 40 ligation on the control of Trypanosoma cruzi infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ligation on MM by CD40L transfected murine L cells or by a soluble CD40L fusion protein up regulated their expression of intercellular adhesion molecule 1 and MHC class 1 and class 2 molecules and their secretion of IL 6 , IL 8 , tumor necrosis factor a , and granulocyte macrophage colony stimulating factor and also induced a rapid activation of the transcription factor nuclear factor kappaB . ^^^ These results indicate that CD 40 CD40L interactions may play an important role in augmenting antitumor immunity and inducing apoptosis in some CD 40 positive immunogenic human MMs . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 and its ligand CD40L are key players in T cell B cell interaction and T cell antigen presenting cell ( APC ) interaction . ^^^ Inhibition of CD 40 CD40L interaction leads to severe humoral and cellular immunodeficiency . ^^^ The highly elevated levels of sCD 40 may point to the involvement of CD 40 and its ligand CD40L in the clinical manifestation of uraemic immunodeficiency . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , it is unclear whether CD 40 CD40L stimulation is able to regulate the production of mycobacterial induced IFN gamma through an IL 12 dependent pathway . ^^^ Together , these studies are the first to demonstrate that BCG induced IFN gamma production by human cells appears to be mediated by IL 12 in a CD 40 dependent manner and suggest that CD 40 CD40L activation may be a critical mediator in regulating the immune response to stimulation with BCG . . ^^^
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We also find that ligation of microglial CD 40 by CD40L triggers a significant production of TNF alpha . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40 ligand ( CD40L ) interaction was originally defined as important molecules for the development of humoral immunity . ^^^ Here we investigated the antitumor activity of murine alveolar macrophages through CD 40 CD40L interaction . ^^^ Stimulation of CD 40 molecules on the surface of alveolar macrophages with 3LLSA CD40L cells induced the production of nitric oxide , tumor necrosis factor alpha , and interleukin 12 and the tumoricidal activity of alveolar macrophages in the presence of interferon gamma , which increased the surface expression of CD 40 molecules on alveolar macrophages . ^^^ On the other hand , interleukin 6 production by alveolar macrophages did not depend on CD 40 CD40L interaction . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In order to understand the mechanism by which ligand : CD 40 interactions augment DC function , we assessed the role of T cell stimulatory cytokines IL 12 and IL 15 in the function of DC stimulated with soluble trimeric CD40L , a recombinant fusion protein incorporating three covalently linked extracellular CD40L domains ( huCD40LT ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here , we have investigated whether protection of NOD mice from IDDM was associated with changes on costimulatory pathways of T and B cells , namely CD28 / CTLA 4 B 7 and CD 40 CD40 ligand ( CD40L ) and we also further characterized protective T helper ( Th ) cells with regards to the expression of the differentiation markers CD45RB and CD 38 . ^^^ The protective effect of Mycobacterium avium infection is also associated with increased CD40L and CTLA 4 expressing Th cells and with the generation of a CD 40 IgG+ B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The ligand for CD 40 ( CD40L ) , an important immunoregulatory molecule , is an example of the successful application of mutants , monoclonal antibodies , and molecular methods to cloning and biological characterization of new molecules . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Engagement of CD 40 and CD40L is also essential for IgE synthesis and secretion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In functional assays , Fas : COMP was at least 20 fold more active than Fas : Fc at inhibiting the action of sFasL , and CD 40 : COMP could block CD40L mediated proliferation of B cells , whereas CD 40 : Fc could not . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Association of TTRAP with CD 40 increases profoundly in response to treatment of cells with CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To investigate the roles of the co stimulatory molecules CD40 / CD40L , CD30 / CD30L , CD27 / CD27L , and OX40 / OX40L , which belong to the tumour necrosis factor ( TNF ) receptor / ligand superfamily , in the development of chronic ongoing myocarditis , the expression of CD 40 , CD30L , CD27L , and OX40L was analysed in the hearts of A / J mice with myocarditis induced by Coxsackie virus B 3 ( CVB 3 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 antigen is a costimulatory molecule highly expressed on dendritic cells ( DC ) and activated B cells , which induces T cell proliferation through the binding with CD40L receptor . ^^^ CD 40 antigen is a costimulatory molecule highly expressed on dendritic cells ( DC ) and activated B cells , which induces T cell proliferation through the binding with CD40L receptor . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we show that high threshold occupancy of CD 40 by its cognate ligand ( CD40L ) promotes rapid induction of CD 77 expression in non GC ( CD 38 ( lo ) ) B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 40 and its ligand , CD40L , in intestinal lesions of Crohn ' s disease . ^^^ Recently , overexpression of the ligand for CD 40 ( CD40L ) , a lymphocyte costimulatory molecule , was shown to induce severe inflammatory bowel disease in transgenic mice . ^^^ In the present study , we examined the expression of CD 40 and CD40L on surgical specimens of ileum from 12 patients with Crohn ' s disease and 10 patients with diverticulitis . ^^^ In addition , several infiltrating macrophages were found to express B 7 1 and B 7 2 molecules , the ligands of CD 28 and CTLA 4 , which cooperate with the CD 40 CD40L pathway in lymphocyte activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 can participate in inflammatory processes after binding its cognate ligand ( CD40L ) . ^^^ These observations suggest a role for CD 40 in central nervous system ( CNS ) inflammation and that CD40 / CD40L autocrine or paracrine pathways may mediate this role . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Furthermore , the addition of triazolopyrimidine to monocytes cultured with IL 4 and GM CSF prevented their differentiation into fully competent dendritic cells ( DC ) as DC differentiated in the presence of triazolopyrimidine expressed less CD 40 at their surface and were profoundly deficient in the production of IL 12 upon exposure to CD40L transfectants . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
For successful active specific immunization against human cancers the understanding of the immunoevasive maneuvers of the tumor cell ( through FasL > Fas ; TRAIL ; CD40L > CD 40 ; TGFbeta etc . systems ) is essential . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Since costimulation is critical for T cell activation , we investigated the role of the CD 28 B7 and CD 40 CD40 ligand ( CD40L ) interactions in this infection induced immunopathology . ^^^ In vivo blockade of the CD 28 B7 or CD 40 CD40L interactions following infection of IL 10KO mice with T . gondii did not affect serum levels of IFN gamma or IL 12 , nor did it prevent death in these mice . ^^^ Analysis of parasite specific recall responses from infected IL 10KO mice revealed that blockade of the CD 40 CD40L interaction had minimal effects on cytokine production , whereas blockade of the CD 28 B7 interaction resulted in decreased production of IFN gamma but not IL 12 . ^^^ Together , these results demonstrate that the CD 28 B7 and CD 40 CD40L interactions are involved in the development of infection induced immunopathology in the absence of IL 10 . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Collectively , these results suggest that the interaction between CD 40 on synovial fibroblasts and CD40L expressed on activated T lymphocytes may be directly involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It is important that CD 40 and its ligand , CD40L , have been implicated in the provision of this help and , in particular , the generation of long lasting CTL memory . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To characterize the involvement of costimulatory pathways in the pathogenesis of myasthenia gravis ( MG ) , a multiparameter flow cytometry assay was adopted to enumerate blood mononuclear cells ( MNC ) expressing CD 28 , CD 80 , CD 86 , CD 40 , and CD40L molecules in patients with MG and healthy subjects . ^^^ Patients with MG had lower percentages of CD 8 ( + ) CD 28 ( + ) cells , augmented percentages of CD 4 ( + ) CD 80 ( + ) , CD 4 ( + ) CD 86 ( + ) , CD 8 ( + ) CD 80 ( + ) , CD 8 ( + ) CD 86 ( + ) , CD 14 ( + ) CD 80 ( + ) , and CD 14 ( + ) CD 86 ( + ) cells , and similar levels of cells expressing CD 40 and CD40L and of B cells expressing CD 80 and CD 86 compared to the controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The enhanced agonistic activity could be attributed to a dose dependent increase in CD40L binding to CD 40 in the presence of SGN 14 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Our data show that both CD 3 ( + ) and CD 3 GLs are preferentially equipped with CD 30 , CD 40 , CD40L , CD 70 , and CD 95 antigens ; this pattern is usually associated with the lack of CD 27 and CD30L antigens expression . ^^^ On functional grounds , a stimulatory activity on rIL 2 mediated redirected cytotoxicity against Fcgamma+ P 815 targets was demonstrated with anti CD 30 , CD 40 , CD40L , CD 70 , CD 95 , and CD95L mAbs , although resting cells were unable to exhibit significant redirected cell lysis . ^^^ The addition of anti CD 30 , CD30L , CD 40 , CD40L , CD 95 , and CD95L mAbs did not show any significant effect on cell proliferation at resting conditions or after rIL 2 stimulation , whereas anti CD 70 mAb mediated cell proliferation in 6 of 10 cases tested . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : CD 40 CD40 ligand ( CD40L ) interaction is essential for the T lymphocyte dependent immune response . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 triggering was performed using soluble CD40L or cocultures with CD40L transfected fibroblasts . ^^^ If the exact molecular mechanisms are unclear , it is likely that CD 40 CD40L interaction could play a role in this process . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Oral administration of the more native fragment , Trx Halpha 1 210 , at the acute phase of disease led to exacerbation of EAMG , accompanied by an elevation of AChR specific humoral and cellular reactivity , increased levels of Th 1 type cytokines ( IL 2 , IL 12 ) , decreased levels of Th 2 ( IL 10 ) or Th 3 ( TGF beta ) type cytokines , and higher expression of costimulatory factors ( CD 28 , CTLA 4 , B 7 1 , B 7 2 , CD40L , and CD 40 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Under selected circumstances , such as in CD 40 KO mice , both CD 4 ( + ) and CD 8 ( + ) T cells mediate hepatocyte rejection in the absence of CD40 / CD40L costimulation and without a significant contribution from CD28 / B7 costimulation signals . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L , but not CD 40 , is required for allergen induced bronchial hyperresponsiveness in mice . ^^^ Interaction of CD 40 on B cells and antigen presenting cells , with its ligand ( CD40L ) expressed transiently on activated T cells , is known to augment both T cell driven inflammation and humoral immune responses , especially IgE production . ^^^ Considering both the prominent role of inflammation in asthma and the association of the disease with IgE , we hypothesized that CD 40 CD40L interactions would be important in pathogenesis . ^^^ To test this hypothesis , we subjected wild type ( WT ) mice and animals lacking either CD 40 or CD40L to repeated inhalation of Aspergillus fumigatus ( Af ) antigen . ^^^ IgE production was markedly suppressed in both the CD 40 / and CD40L / strains . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Macrophages , smooth muscle cells , endothelial cells , and T cells express CD 40 and CD40L in fatty streaks and more advanced human atherosclerotic lesions . ^^^ CD 40 CD40L receptor ligand interaction plays a central role in antigen presentation , immunological reactions , and in T cell and macrophage activation . ^^^ Since all these mechanisms are important for the pathogenesis of atherosclerosis , we studied the expression profile of CD 40 CD40L in different types of human atherosclerotic lesions using double immunostaining techniques with cell type specific antibodies . ^^^ Normal human intima did not contain CD 40 or CD40L immunoreactivity . ^^^ From type 2 lesions ( fatty streaks ) to advanced type 6 lesions ( complicated plaques ) , colocalization of CD 40 and CD40L was observed in T cells ( CD3+ cells ) , macrophages ( CD68+ cells ) , and smooth muscle cells ( HHF35+ cells ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Unexpectedly , C57BL / 6 mice but not CD 40 ligand deficient ( CD40L ( / ) ) mice adoptively transferred with AAV lacZ infected immature DCs developed a beta galactosidase specific cytotoxic T lymphocyte ( CTL ) response that markedly diminished AAV lacZ transduced gene expression in muscle fibers . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Upon activation , T cells express CD40L , a member of the TNF cytokine superfamily , which serves as a ligand for CD 40 on antigen presenting cells , including dendritic cells , B cells , and macrophages . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the current study we examined the role of CD 40 mediated interactions in immune responses to bladder , pancreatic and breast carcinomas as well as melanoma cell lines using soluble human CD40L ( rhCD40L ) or anti CD 40 mAb in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Thus , this M cell associated phenotype might result from B T cell interactions in the course of antigen presentation by memory B cells , with subsequent CD 40 engagement by CD40L expressing cognate memory T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Morphological features and mechanisms of cytokine production resemble those observed in microglia T cell co cultures since CTLA 4 and CD 40 CD40L blockades reduce TNF alpha and IL 10 levels , while anti CD 23 inhibits IL 10 only in U 937 T cell interactions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Memory B cell development is impaired by in vivo blockade of the CD 40 CD40 ligand ( CD40L ) interaction using human Fc immunoglobulin G 1 ( IgG 1 ) mouse CD 40 fusion protein ( CD 40 Ig ) ; however , germinal centre ( GC ) formation is not . ^^^ This increase in apoptosis is not caused by a blockade of the CD40L mediated rescue signal but is the result of an intrinsic defect of GC B cells in CD 40 Ig treated mice to receive rescue signals via CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Extended studies demonstrated that : 1 ) ASM cells express mRNAs and proteins for the cell adhesion molecules ( CAMs ) / costimulatory molecules , CD 40 , CD40L , CD 80 , CD 86 , ICAM 1 ( CD 54 ) , and LFA 1 ( CD11a / CD18 ) ; 2 ) apart from LFA 1 , ASM cell surface expression of the latter molecules is up regulated in the presence of activated T cells ; and 3 ) pretreatment of ASM cells and tissues with mAbs directed either against CD11a or the combination of CD 40 and CD 86 completely abrogated both the activated T cell induced changes in expression of the above CAMs / costimulatory molecules in ASM cells and altered ASM tissue responsiveness . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The gene and protein expression of essential haematopoietic growth factors ( IL 2 , IL 4 , IL 7 , IL 10 , IL 15 , IFN gamma , G CSFR ) , their corresponding receptors as well as the expression pattern of adhesion molecules ( ICAM 1 , CD 58 ) and costimulatory proteins ( CD 40 , CD40L , B7 . 1 , B7 . 2 , CD 28 , MHC 1 and 2 ) was analyzed by RT PCR and flow cytometry . ^^^ Furthermore , high CD 40 , and , surprisingly , CD40L expression could be found . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Isolated peripheral blood lymphocytes were stained for the lymphocyte subset markers CD 4 , CD 8 , CD 19 , their respective activation markers CD 25 , HLA DR , CD 38 , and the costimulatory molecules CD40L , CD 28 , CD 40 , CD 80 , and CD 86 . ^^^ No changes in CD 40 or CD40L expression were found in the patients with SLE . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
MATERIALS AND METHODS : CD40L expression was induced in a CD 40 ( + ) ( RPMI 8226 ) and a CD 40 ( ) ( U266B1 ) human myeloma cell line ( HMCL ) by adenoviral vector gene transfer . ^^^ RESULTS : CD40L expression significantly inhibited the growth of the CD 40 ( + ) HMCL and induced apoptosis . ^^^ Where the tumor cells are CD 40 ( + ) , the engagement of CD 40 antigen by CD40L on tumor cells induces their apoptosis , allowing uptake of tumor associated antigen by professional APC . ^^^ Independently of tumor cell expression of CD 40 , transgenic expression of CD40L on tumor cells allows them to stimulate CD 40 ( + ) APC , to increase their maturation and their capacity to stimulate cytotoxic T lymphocytes ( CTL ) that recognize the tumor derived antigens the APC may have engulfed . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
After human monocytes were cultured for 24 hours in serum free medium and granulocyte macrophage colony stimulating factor to produce immature DCs , additional overnight culture with either calcium ionophore ( CI ) or interferon gamma ( IFN gamma ) , tumor necrosis factor alpha ( TNF alpha ) , and soluble CD40L resulted in phenotypically mature DCs that produced interleukin 8 ( IL 8 ) and displayed marked expression of CD 80 , CD 86 , CD 40 , CD 54 , CD 83 , DC LAMP , and RelB . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expressions of CD 54 , CD 58 , CD11a / CD18 , CD 40 , CD40L , CD 56 , B 7 2 ( CD 86 ) , HLA 1 and HLA 2 molecules were not affected by the transfection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Primary cultures of human BEC also expressed Fas , FasL , and CD 40 but not CD40L protein or mRNA . ^^^ Activation of CD 40 on BEC using recombinant CD40L increased transcriptional expression of FasL and induced apoptosis , which was inhibited by neutralizing antibodies to either Fas or FasL . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OBJECTIVE : To investigate the protective effects of blocking CD40 / CD40L interactions with human CD 40 Ig fusion protein in a murine graft versus host disease model . ^^^ Purified CD 40 Ig could bind to CD40L . ^^^ CONCLUSIONS : These results demonstrate that CD40 / CD40L interactions play an important role in the pathogenesis of graft versus host disease and suggest clinical potential for CD 40 Ig in the prevention and treatment of human graft versus host disease . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The purpose of the present study was to investigate the presence and distribution of CD 23 , CD 21 , CD 40 and CD40L in the conjunctiva from patients with active VKC . ^^^ We used immunohistochemical techniques and a panel of monoclonal antibodies ( mAbs ) directed against CD 23 , CD 21 , CD 40 and CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 ' s association with CD40L activates signaling . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Our data suggest that CD 40 expression in carcinoma cells might convert a CD40L dependent immunological defense signal into a tumor promoting signal . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40L interactions induce chemokine expression by human microglia : implications for human immunodeficiency virus encephalitis and multiple sclerosis . ^^^ CD 40 is a protein on microglia that is up regulated with interferon ( IFN ) gamma and is engaged by CD40L , found on CD4+ T cells , B cells , and monocytes . ^^^ We examined the expression of CD 40 on microglia in human immunodeficiency virus ( HIV ) encephalitic brain , and the effects of CD 40 CD40L interactions on the expression of chemokines by cultured microglia . ^^^ Our data suggest a mechanism whereby CD40L+ cells can induce microglia to secrete chemokines , amplifying inflammatory processes seen in HIV encephalitis and multiple sclerosis , and implicate CD 40 CD40L interactions as a target for interventional strategies . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L , a member of the tumor necrosis factor family of ligands , plays a major role in immune responses via its receptor , CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : The interaction between CD 40 on antigen presenting cells and CD40L on T cells is critical in allograft rejection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
IDCs from monkeys with AIDS were refractory to stimulation with CD40L , demonstrating a functional consequence of decreased CD 40 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interactions of T cell surface receptors CD 28 and CD40L with their ligands B 7 and CD 40 , respectively , on APCs are critical for antigen specific T cell activation under physiological and pathological conditions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we show that CD40L ( ligand for CD 40 ) failed to induce the production of tumour necrosis factor alpha ( TNF alpha ) , interleukin ( IL ) 1 beta , IL 10 and IL 12 in macrophages matured in vitro in the absence of growth factors or in the presence of macrophage colony stimulating factor ( M CSF ) . ^^^ The priming effect of CD40L on TNF alpha and IL 1 beta production was related to induction of CD 40 expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40L interactions in atherosclerosis . ^^^ Increasing evidence supports a central role for CD 40 CD40L interactions in the pathogenesis of atherosclerosis . ^^^ CD 40 and CD40L protein are present in almost all cell types in human atherosclerotic lesions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
PURPOSE : To determine whether CD 40 CD40 ligand ( CD40L ) interaction plays a role in corneal inflammatory responses , the expression of CD 40 and CD40L on normal human cornea was investigated . ^^^ METHODS : Frozen optimal cutting temperature ( OCT ) compound embedded sections of corneal tissues obtained from 18 normal human corneas were examined by an immunoperoxidase staining technique with anti CD 40 and anti CD40L monoclonal antibodies ( mAbs ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Up regulated surface expression of CD 40 did not correlate with IL 12p70 production , and appreciable CD40L induced IL 12p40 secretion was observed in uninfected as well as infected DCs , regardless of species . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40L interaction in Alzheimer ' s disease . ^^^ Increasing evidence supports a role of the CD 40 receptor CD 40 ligand ( CD 40 CD40L ) interaction in the pathogenesis of Alzheimer ' s disease ( AD ) . ^^^ This suggests that the CD 40 CD40L system is a critical enhancer of microglial activation in an AD transgenic mouse model and that such activation is associated with an increase in a key indicator of neuronal stress . ^^^ Conversely , the finding that reduced CD 40 CD40L interaction is associated with reduced chronic microgliosis and Tau hyperphosphorylation supports the view that , in general , mechanisms that reduce microgliosis will be beneficial in AD . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In order to determine their biological potential , these antibody fragments have been analysed for their ability to promote B cell activation , rescue from apoptosis and to block the CD 40 CD40 ligand ( CD40L ) interaction . ^^^ Thus scFv were generated that induced B cell proliferation , rescued B cells from apoptosis and blocked the CD 40 CD40L interaction to different extents . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Tolerance induction of alloreactive T cells via ex vivo blockade of the CD 40 : CD40L costimulatory pathway results in the generation of a potent immune regulatory cell . ^^^ We previously reported that ex vivo blockade of the CD 40 : CD40L costimulatory pathway in primary mixed lymphocyte reaction cultures resulted in profound in vitro secondary hyporesponsiveness and 30 fold or greater protection from graft versus host disease ( GVHD ) lethality . ^^^ These data , the first to demonstrate that tolerance induction via CD 40 : CD40L costimulatory blockade results in potent regulatory function , are relevant to bone marrow and solid organ transplantation . ^^^ The generation of potent immunoregulatory capacity during tolerization via CD 40 : CD40L blockade provides a fail safe mechanism to control alloreactive T cells that may have escaped tolerization . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The importance of cell surface molecules was examined by application of a panel of blocking antibodies ( CD 2 , CD 18 , CD 40 , CD40L , CD 54 , CD 58 ) in addition to use of TCR negative T cell lines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We confirmed that a CD 8 ( + ) effector cell was required to eliminate MUC 1 expressing Panc 02 tumors , and demonstrated that T cells expressing TCR alpha / beta and co stimulation through CD 28 and CD 40 : CD40L interactions played critical roles during the initiation of the anti Panc02 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40 ligand ( CD40L ) interaction is an important costimulatory signal in the interaction between T cells and antigen presenting cells ( APC ) . ^^^ We showed that bone marrow derived DC pulsed with Id CD40L upregulated the expression of CD 40 , CD 80 , CD 86 , and major histocompatibility complex ( MHC ) class 2 molecules with the increased production of interleukin 12 ( IL 12 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD40L induces proliferation , self renewal , rescue from apoptosis , and production of cytokines by CD 40 expressing AML blasts . ^^^ In the present study , we analyzed the capability of CD40L / CD154 to modulate several functional aspects of CD 40 expressing AML blasts . ^^^ METHODS : After defining the constitutive expression levels of CD 40 in a wide panel ( n = 67 ) of AMLs and evaluating the capability of cytokines to modulate its expression , we investigated the effects of CD 40 engagement by soluble ( s ) CD40L on proliferation , self renewal capacity , apoptosis , homotypic adhesion , and cytokine production of leukemia cells . ^^^ CONCLUSIONS : This study indicates a possible involvement of CD 40 in the interactions of AML blasts with other growth sustaining microenvironmental accessory cells and immune effectors , in turn expressing CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that cross linking CD 40 , using either soluble CD40L ( sCD40L ) or anti CD 40 monoclonal antibody ( mAb ) , induces phosphatidylinositol 3 kinase ( PI3K ) activity and activates its downstream effector AKT in MM . 1S cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Th cells to a large extent operate through up regulation of CD40L , which then interacts with CD 40 on DC to cause DC maturation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interestingly , 4 1BB mediated stimulation of immune responses in CD40L ( / ) mice is effective ( although at a reduced level ) , and may suggest the existence of an alternative ligand for CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Finally , systemic administration of FLT3L and / or CD40L reversed CD 40 and IL 12 ( p 40 ) deficiency of DCs in tumor bearing mice in vivo . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : CD40L CD 40 interactions induce inflammatory signals in cells of the vascular wall . ^^^ On endothelial cells , induction of proteolytic activity by activated platelets was mimicked by CD 40 engagement using soluble CD40L but not affected by antibody clustering of alpha ( 5 ) beta 3 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Involvement of CD 40 CD40L signaling in postischemic lung injury . ^^^ Immunoneutralization of either CD 40 or CD40L , cell surface proteins important in lymphocyte endothelial cell proinflammatory events , results in significantly lower postischemic K ( f , c ) values . ^^^ Antagonism of CD 40 CD40L signaling also results in attenuation of I / R elicited macrophage inflammatory protein 2 production . ^^^ In addition to lymphocyte involvement via CD 40 CD40L interactions , our studies also show that I / R injury is potentiated by antagonism of IL 10 produced locally within the postischemic lung , whereas exogenous , rat recombinant IL 10 provided protection against I / R induced microvascular damage . ^^^ Thus acute lymphocyte involvement in lung I / R injury involves CD 40 CD40L signaling mechanisms , and these events may be influenced by local IL 10 generation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 antigen and its ligand ( CD40L ) have been shown to play a major role in regulating both humoral and cellular immune responses . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We investigated in hepatoma cell lines the regulation of CD 40 by pro inflammatory cytokines and the effects of its ligation with soluble CD40L on the expression of co stimulatory and pro apoptotic cell surface molecules and survival . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Immunohistochemistry was used to examine expression of BCL 2 , BAX , P 53 , CD 40 and CD40L in archival tissues of patients included in various treatment trials for transitional cell carcinoma ( TCC ) of the bladder . ^^^ Median survivals for the various markers were as follows : BAX positive patients 110 m vs BAX negative patients 18 m ( P=0 . 0002 ) ; CD40L positive patients 95 m vs CD40L negative patients 45 m ( P=0 . 04 ) ; BCL 2 positive patients 44 m and BCL 2 negative patients 74 m , ( P=0 . 64 ) ; CD 40 positive patients 110 m and CD 40 negative patients 45 m ( P=0 . 12 ) ; and P 53 positive patients 80 m and P 53 negative patients 45 m ( P=0 . 58 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Using both acute blockade with systemic administration of antibodies and mice genetically deficient in the costimulatory molecules CD 28 , CD 40 , and CD40L , we have delineated the requirements of costimulation required to reject this virally induced endothelial tumor . ^^^ Mice genetically deficient in CD 28 , CD 40 , and CD40L were able to reject SVR tumors , but depletion of these mice of CD 8 , but not CD 4 cells led to rapid tumor growth . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Oxidized low density lipoprotein augments and 3 hydroxy 3 methylglutaryl coenzyme A reductase inhibitors limit CD 40 and CD40L expression in human vascular cells . ^^^ METHODS AND RESULTS : Treatment of human vascular endothelial and smooth muscle cells and mononuclear phagocytes with oxLDL augmented the basal expression of CD 40 and CD40L mRNA and protein . ^^^ CONCLUSIONS : OxLDL may promote expression of CD 40 and CD40L in human atheroma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent evidence suggests that interactions between CD 40 on antigen presenting cells ( APC ) and CD40L on T cells generate signals that result in the activation of APC . ^^^ The expression of CD 40 by cerebral endothelium , and endothelial cell activation following binding of CD 40 to its ligand , CD40L , suggest a potential mechanism by which activated CD40L expressing T cells could enhance adhesion and migration of inflammatory cells across the blood brain barrier ( BBB ) to sites of inflammation in the human central nervous system ( CNS ) . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CONCLUSIONS : The results demonstrate that human CD 34 ( + ) blood progenitors stimulate T cell responses potently and can induce T cell unresponsiveness only when both B 7 : CD 28 and CD 40 : CD40L pathways are blocked , with an increase of interleukin 10 producing cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Production of MIP 1alpha by synovial fibroblasts is significantly increased by engagement of CD 40 with CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Frozen sections of kidney tissue from 10 patients with lupus nephritis and two non SLE patients ( with minimal change disease ) were stained with monoclonal antibodies for interleukin ( IL ) 4 , IL 10 , IL 12 , IFN gamma , CD 4 , CD 8 , CD 40 , CD 68 and CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Although previous studies demonstrated a requirement for CD 40 CD40 ligand ( CD40L ) interaction in the development of resistance to Leishmania infection , we recently showed that mice lacking the gene for CD40L ( CD40L ( / ) mice ) can control Leishmania major infection when they are infected with reduced numbers of parasites . ^^^ In this study , we examine the cytokine pattern in healing versus nonhealing CD40L ( / ) mice and investigated whether CD 40 activation is required for resistance to reinfection . ^^^ Together , these results show that CD 40 CD40L interaction , although important for IL 12 production in high dose infections , is not required for either the development or maintenance of resistance in mice infected with reduced numbers of parasites . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS AND RESULTS : We observed that ox LDL increased the expression of CD 40 and CD40L in a concentration ( 10 to 80 microg / mL ) and time ( 1 to 24 hours ) dependent manner . ^^^ These effects of ox LDL were mediated by activation of LOX 1 , because pretreatment of HCAECs with a blocking antibody to LOX 1 ( JTX 92 ) prevented the expression of CD 40 and CD40L in response to ox LDL ( P < 0 . 01 ) . ^^^ The PKC inhibitor also blocked the effects of ox LDL on the expression of CD 40 and CD40L ( P < 0 . 01 ) . ^^^ In additional experiments , we found that it is the PKCalpha , but not PKCbeta and PKCgamma , isoform that mediated ox LDL induced CD 40 and CD40L upregulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To prevent this response , we used an anti CD40L monoclonal antibody , MR 1 , which blocks the costimulatory interaction between CD 40 and CD40L . ^^^ These findings suggested that gene therapies for recessive diseases may provoke an immune response against the transgene product , and that the CD 40 CD40L interaction might be a reasonable target for effective prevention of such undesirable immune responses , leading , in turn , to a successful gene therapy . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We hypothesized that in relapsing remitting MS ( RRMS ) ( with or without of IFNbeta treatment ) and in secondary progressive patients ( SPMS ) IL 12 and costimulatory molecules ( CD 80 [ B 7 1 ] , CD 86 [ B 7 2 ] , CD 28 , CD 40 , CD40L ) would be differentially produced or expressed by MO or T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It is induced upon engagement of CD 40 on B lymphocytes by CD40L expressed by activated CD4+ T cells and exposure of B cells to T cell secreted cytokines including interleukin 4 and transforming growth factor beta . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Expression of CD 36 , CD40L , and CD 40 was increased in lungs of beta 3 / LDLR / mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 40 CD40L interaction plays a critical role in both humoral and cellular immune responses and interfering antibodies have been suggested as an effective approach for the treatment of lymphomas and autoimmune diseases . ^^^ In this study we have profiled a panel of mouse antihuman CD 40 monoclonal antibodies ( MoAbs ) , regarding their CD 40 binding affinity and epitope specificity relative to the CD40L binding in relation to their cellular activating potential . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The altered responsiveness of activated memory B cells to CD40L and BAFF correlated with changes in surface phenotype such that expression of CD 40 and BAFF R were reduced on ISCs while BCMA was induced . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Understanding the role of the CD 40 CD40L interaction in resistance to parasitic infections . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The results showed that CD 22 , CD 20 , CD62L , CD 40 , CD 24 , CD79b , CD79a , and FMC 7 were almost positive in the circulating B cell population , whereas CD11a , CD 80 , CD 103 , CD 10 , CD40L , CD 54 , CD95L , CD 86 , and CD 95 were almost negative in the peripheral blood B lymphocytes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CONCLUSION : The CD 40 CD40L pathway augments mucosal inflammatory responses via mucosal PCLF . ^^^ CD 40 CD40L mediated T cell / PCLF interactions could play an important role during intestinal mucosal inflammation . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To prevent the alloimmune response , we used blockade of CD 28 : B 7 and CD40L : CD 40 costimulatory signaling pathways to determine the effect of hyperglycemia alone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We measured CTL responses induced in vivo with antigenic peptides P 198 and P1A loaded onto bone marrow derived DCs that had matured as a consequence of CD 40 CD40L interactions . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of platelet CD 40 with recombinant soluble CD40L trimer ( sCD40LT ) caused increased platelet CD62P expression , alpha granule and dense granule release , and the classical morphological changes associated with platelet activation . ^^^ These actions were abrogated by the CD40L blocking antibody TRAP 1 and the CD 40 blocking antibodies M 2 and M 3 , showing that activation was mediated by CD40L binding to platelet CD 40 . beta 3 integrin blockade with eptifibatide had no effect , indicating that outside in signaling via alphaIIbbeta 3 was not contributing to these CD 40 mediated effects . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
To improve the efficacy of tumor cell based and dendritic cell ( DC ) based cancer vaccines , this study explored the potential of a new cancer vaccine strategy , that is , the use of CD 40 ligand transfected tumor ( CD40L tumor ) cells to simultaneously deliver both tumor derived antigens ( Ag ) and maturation stimuli to DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 , but not CD40L , is required for the optimal priming of T cells and control of aerosol M . tuberculosis infection . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Previous studies have demonstrated that the antiplatelet agent trapidil has multiple actions , including suppression of MMP expression through the inhibition of the CD 40 CD40 ligand ( CD 40 CD40L ) pathway in cultured cells . ^^^ CONCLUSIONS : Trapidil , a CD 40 CD40L pathway inhibitor , suppressed mRNA expression and protein production of MMP 2 in AAA tissues , suggesting a potential therapeutic approach for the prevention or treatment of AAA . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Cross talk between CD 40 and CD40L : lessons from primary immune deficiencies . ^^^ Furthermore , the importance of CD40 / CD40L interaction for the induction of adaptive immunity will be outlined in the context of primary immunodeficiencies due to defects of the genes involved in the CD 40 signalling pathway , which are characterized by an immunological phenotype of hyper IgM syndrome . ^^^ SUMMARY : The critical role of CD40 / CD40L interactions in the development of various disease states has been fully appreciated , and further understanding of the molecular events involved in CD 40 signalling may allow the identifications of candidate genes for other hyper IgM syndromes . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 and CD40L expression in the chicken embryo aorta : possible role in the endothelial mesenchymal transdifferentiation process . ^^^ Given the important role played by the CD 40 CD40 ligand ( CD40L ) system during the initiation and progress of atherosclerosis , we investigated whether both CD 40 and CD40L were present in the aortic wall during EMT and the advanced stages of chicken embryo development . ^^^ CD 40 CD40L expression was found on endothelial cells ( ECs ) , mesenchymal cells , and smooth muscle cells ( SMCs ) at all stages examined , and appeared to be distributed across the aortic wall . ^^^ CD 40 had a more restricted distribution compared to CD40L , and did not stain every cell type of the aortic wall . ^^^ Specifically , CD40L was associated to the surface of cells that were detaching and migrating from the monolayer of ECs , whereas for CD 40 a very diffuse subcellular localization was seen at the monolayer and the detaching and migrating cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
As the CD 40 CD40L signaling pathway is implicated in the modulation of inflammatory responses between vascular cells , involving adhesion molecules , pro inflammatory cytokines , chemokines , we sought to investigate the potential role of statins in regulating the expression of CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These responses were also augmented by coexpression of T cell costimulatory molecules ( RANKL and CD40L ) or DC costimulatory molecules ( RANK and CD 40 ) in the E 7 expressing DC immunogens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These data implicate the CD 40 : CD40L interaction as a key checkpoint in the development of T cell immunity rather than tolerance . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS AND RESULTS : C reactive protein ( CRP ) , soluble ( s ) CD40L , and surface expression of CD40L on platelets and T cells and of CD 40 on monocytes and platelet monocyte aggregates were compared in 25 cigarette smokers and 25 age and gender matched nonsmokers . ^^^ Smokers also had increased surface expression of CD 40 on monocytes ( 45 . 9+ / 7 . 7 % versus 39 . 9+ / 6 . 5 % , P=0 . 006 ) , of CD40L on platelets ( 2 . 9+ / 1 . 0 % versus 2 . 3+ / 0 . 6 % , P=0 . 03 ) , and of platelet monocyte aggregates ( 26 . 6+ / 10 . 9 % versus 19 . 7+ / 8 . 6 % , P=0 . 02 ) . ^^^ Plasma cotinine concentrations correlated with monocyte CD 40 expression , platelet CD40L expression , and platelet monocyte aggregates . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
It has been proposed that long term maintenance of CTL memory and control of lymphocytic choriomeningitis virus ( LCMV ) is dependent upon the presence of T helper cells and interaction of antigen presenting cells and CTL via CD 40 and its ligand CD40L . ^^^ However , we demonstrate here that CD 40 CD40L interaction maintains CTL memory by induction of virus specific antibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Importantly , they become fully competent antigen presenting cells ( APC ) after a short maturation induced by IL 3 + CD40L or virus , exhibiting a characteristic APC phenotype ( high expression of CD 83 and of the costimulatory molecules CD 40 , CD 80 , CD 86 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The expression of CD 40 and CD40L on platelet was analyzed by flow cytometry . ^^^ RESULTS : Patients with ACS showed a significant increase of CD 40 ( 75 + / 12 MIF ) and CD40L ( 13 + / 4 MIF ) coexpression on platelets compared with control and SA group ( P < 0 . 01 ) . sCD40L also showed higher level in patients with ACS ( 10 . 2 + / 3 . 5 microg / L ) than in control ( 3 . 1 + / 1 . 4 microg / L , P < 0 . 01 ) and SA group ( 3 . 3 + / 1 . 6 microg / L , P < 0 . 01 ) . ^^^ A positive correlation was found between MMP 9 , MMP 3 , and CD40L expression on platelets as well as sCD40L levels , but not for CD 40 expression on platelets . ^^^ CONCLUSION : Patients with ACS show increased coexpression of CD 40 system , especially expression of CD40L , which may create a proinflammatory and prothrombotic milieu for aggravating the development of atherosclerosis and instability of atherosclerotic plaques , and may be a valuable marker for predicting the severity of ACS . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The importance of CD 40 CD40L interactions during CD 4 ( + ) T cell activation has been extensively investigated over the years ; however , it still remains questionable whether the interaction is a prerequisite for dendritic cell ( DC ) mediated antigen specific priming in vivo . ^^^ It is well known that the expression of CD80 / CD86 is upregulated after interaction between CD 40 on APCs and CD40L expressed by at least partly activated T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 28 : B 7 families of molecules and the CD 40 : CD40L pair of molecules are considered as critical costimulatory elements . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The B cells in the lesions become activated through the interaction between CD40L and CD 40 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Costimulation through the CD 40 CD40 ligand ( CD40L ) pathway is critical to allograft rejection , in that anti CD40L mAb therapy prolongs allograft survival . ^^^ However , the majority of studies exploring CD 40 CD40L interactions have targeted CD40L . ^^^ Further , as CD 40 is not required for rejection in BALB / c recipients , but anti CD40L mAb prolongs graft survival in these mice , these results suggest that anti CD40L therapy functions at a level beyond disruption of CD 40 CD40L interactions . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
HLA DR , ICAM 1 , CD 40 , CD40L , and CD 86 are incorporated to a similar degree into clinical human immunodeficiency virus type 1 variants expanded in natural reservoirs such as peripheral blood mononuclear cells and human lymphoid tissue cultured ex vivo . ^^^ The insertion of host derived HLA DR , intercellular adhesion molecule 1 ( ICAM 1 ) , CD 40 , CD40L , and CD 86 within HIV 1 particles was evaluated by using specific antibodies linked to a solid matrix to capture ultrafiltrated viral progeny . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Surprisingly , DCs from CD 40 ( / ) and CD40L ( / ) mice did not elicit CD 4 ( + ) and CD 8 ( + ) T cell immunity , even though the DCs exhibited presented ovalbumin on major histocompatibility complex class 1 and 2 products and expressed high levels of CD80 / 86 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The gammadelta T cell effect upon the LNC response to MBP did not involve a change in expression of the costimulatory molecules CD 28 , CD40L , and CTLA 4 on TCRalphabeta ( + ) cells , and CD 40 , CD 80 , and CD 86 on CD 19 ( + ) and CD11b ( + ) cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Surprisingly , generation of proliferation competent memory CTLs was completely independent of CD 40 or CD40L , molecules commonly assumed to be central for mediating the beneficial effects of Th cells on CTL development . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These results indicate that PS liposomes encapsulating antigens could enhance antigen dependent IL 12 production through the interaction between CD 40 on macrophage / dendritic cells and CD40L on 42 6A cells . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In this study , we examined the contribution of CD 40 CD40 ligand ( CD40L ) interaction in the development of intestinal muscle hypercontractility , in monocyte chemoattractant protein 1 ( MCP 1 ) production , and in the Th 2 response in CD 40 ligand deficient ( CD40L / ) mice infected with T . spiralis . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The IHS markers demonstrated positive staining with Bax p 53 , CD 40 and CD40L in AR treated cells and negative staining with Bcl 2 . 5 FU treated cells demonstrated a profile similar to AR but with less intensity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40L interactions are critical for conditioning dendritic cells ( DC ) and priming T and B cell immunity . ^^^ We observed a high level of CD40L protein on purified viral particles and demonstrated that induction of IL 12 by nonreplicating rV CD40L was blocked by anti CD 40 antibodies suggesting that functional CD40L on viral particles contributed to alterations in IL 12 synthesis . ^^^ Infected cells stimulated IL 12 secretion by DC and proliferation of B cells and DX 5 ( + ) ( NK / NKT ) cells through direct CD 40 CD40L interaction . ^^^ A subpopulation of NKT cells expressing CD 40 ( NK1 . 1 ( + ) , CD 3 ( lo ) ) appeared to be a major effector population responding to MC38 / rV CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Coexpression of CD 40 and CD40L on B lymphoma and carcinoma cells : an autocrine anti apoptotic role . ^^^ CD 40 was expressed on T47D and BT 20 breast carcinoma cell lines while CD40L was detectable on T47D ( 93 % , MFI 137 ) only . ^^^ Both BT 20 and T47D had detectable CD 40 mRNA , while CD40L mRNA was detectable only in the T47D cell line . ^^^ CD 40 , but not CD40L , was detectable on 6 renal , 1 prostatic and 1 colon carcinoma cell lines . ^^^ CD40L expressed on tumor cells was functional , as shown by its capacity to decrease drug induced apoptosis on CD 40 expressing NHL and breast carcinoma cell lines , while irradiated CD40L negative cell line ( BT 20 ) had no effect . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
C reactive protein induced expression of CD 40 CD40L and the effect of lovastatin and fenofibrate on it in human vascular endothelial cells . ^^^ CD 40 CD40L system is proven to be an important mediator of several auto immune and chronic inflammation diseases . ^^^ Interruption of CD 40 CD40L signaling pathway not only reduces the initiation and progression of atherosclerotic lesions , but also modulates plaque architecture . ^^^ By using a flow cytometry and western blotting , we found that incubation of human umbilical vein endothelial cells ( HUVECs ) with CRP resulted in a time and dose dependent increase in the cell surface expression of CD 40 and CD40L . ^^^ Furthermore , lovastatin ( 10 ( 7 ) , 10 ( 6 ) , 10 ( 5 ) mol / l ) and fenofibrate ( 5 10 10 ( 5 ) , 10 ( 4 ) , 2 10 10 ( 4 ) mol / l ) significantly diminished the expression of CD 40 , CD40L and gelatinase activities ( MMP 2 , MMP 9 ) induced by CRP in HUVECs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
MSC were negative for MHC 2 , CD 40 , CD40L , CD 80 ( B 7 1 ) and CD 86 ( B 7 2 ) , positive for MHC 1 , and kept this expression pattern during osteogenic differentiation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The authors examined the effect of the quinazoline based alpha 1 adrenergic receptor antagonists bunazosin , doxazosin , prazosin , and terazosin on the expression of adhesion molecules such as ICAM 1 , B7 . 1 , B7 . 2 , CD 40 , and CD40L on monocytes isolated from human peripheral blood mononuclear cells . ^^^ Doxazosin , prazosin , and terazosin induced the expression of ICAM 1 and CD 40 but had no effect on the expression of B7 . 1 , B7 . 2 , and CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that ligation of CD 40 by CD40L modulates Abeta induced innate immune responses in microglia , including decreased microglia phagocytosis of exogenous Abeta ( 1 42 ) and increased production of pro inflammatory cytokines . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Differential downstream effects of CD 40 ligation mediated by membrane or soluble CD40L and agonistic Ab : a study on purified human B cells . ^^^ With the addition of various cytokines , the CD 40 CD40 ligand ( CD40L ) system can act as a T helper cell surrogate to permit B lymphocytes to produce large amounts of polyclonal Ig . ^^^ In the present study , we tested six CD 40 CD40L stimulation models : ( 1 , 2 ) soluble agonistic 89 and G28 . 5 mAbs ; ( 3 , 4 ) 89 and G28 . 5 bound via their Fc fragments on CDw 32 transfected mouse fibroblasts ; ( 5 ) purified , soluble , trimeric human CD40L molecules ( sCD40L ) ; and ( 6 ) human CD40L expressed by a CD40L transfected mouse fibroblastic cell line ( LCD40L ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , ligation of CD 40 on T cells with recombinant CD40L elicited NF kappaB activation , suggesting that the CD 40 pathway is intact and may participate in a positive regulatory loop in T cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
A comparable and significant enhancement of gene transfer to monocyte derived DCs ( MDDCs ) was accomplished by means of an Ad vector harboring the Fc binding domain of Staphylococcus aureus protein A in combination with antibodies to DC SIGN or to CD 40 or with fused complexes of human Ig Fc with their natural ligands , i . e . , ICAM 3 or CD40L , respectively . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We examined whether antibodies ( Abs ) against intercellular adhesion molecule ( ICAM ) 1 , B 7 , CD 40 , and CD 40 , ligand ( CD40L ) affect the cytokine production and T cell proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Haplotype structure of TNFRSF 5 TNFSF5 ( CD 40 CD40L ) and association analysis in systemic lupus erythematosus . ^^^ Seven single nucleotide polymorphisms ( SNPs ) in CD 40 and eight SNPs in CD40L were looked at for linkage disequilibrium ( LD ) and haplotype analysis in European Caucasians . ^^^ Limited haplotype diversity was observed across CD 40 and CD40L , and > 97 % of the diversity was captured . ^^^ We also examined the association of SNPs and haplotypes in CD 40 and CD40L with SLE in European Caucasians . ^^^ There was no evidence of association for CD 40 or CD40L in 408 European Caucasian families with SLE from UK . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 engagement with CD40L significantly blunted the ability of PPARgamma agonists to induce apoptosis of B lymphocytes and prevented the inhibition of NF kappaB mobilization by 15 deoxy Delta ( 12 , 14 ) PGJ ( 2 ) and ciglitazone . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS AND RESULTS : Intravital fluorescence microscopy was used to visualize the cerebral microcirculation of wild type ( WT ) , CD 40 deficient , and CD40L deficient mice subjected to 1 hour MCAO and 4 hour reperfusion . ^^^ Blood cell recruitment and increased permeability were blunted in both CD 40 deficient and CD40L deficient mice . ^^^ Infarct volume was also reduced in CD 40 and CD40L deficient mice compared with WT mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
There is evidence that have shown that CD 40 CD40L interaction plays a pathogenic role in inflammatory disorders . ^^^ The expression of CD 40 and CD40L on platelet was analyzed by indirect immunofluorescence flow cytometry and soluble CD40L level was determined by a commercially available ELISA . ^^^ RESULTS : All patients with hypertension showed a significant increase of CD 40 ( 67 . 1+ / 9 . 6 Mean Fluorescence Intensity , MFI ) and CD40L ( 15 . 3+ / 5 . 0 MFI ) coexpression on platelets as well as sCD40L levels ( 12 . 8+ / 3 . 9 ng / ml ) compared with controls ( p < 0 . 0001 ) . ^^^ We found that CRP levels related to CD 40 CD40L system . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Glioma cell lines expressing low levels of CD 40 at the cell surface resist cytotoxic effects of CD40L . ^^^ CD 40 gene transfer sensitizes glioma cells to CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40 ligand ( CD40L ) signaling stimulates DC mediated IL 12 production / cytotoxicity . ^^^ Strategies to bypass / augment CD 40 CD40L signaling may improve current NB immunotherapies . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
These results demonstrate cross talk between CD 40 and BCR , such that the requirements for BCR signaling are altered by prior B cell exposure to CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The CD 28 : B 7 and the CD 40 : CD40L families of molecules are considered to be critical costimulatory molecules and have been studied extensively . ^^^ In this paper , we review the role of costimulatory molecules in autoimmune diseases , two of the most well studied costimulatory pathways ( CD28 / CTLA 4 : B 7 and CD 40 : CD40L ) , and the advantages of peptidomimetic approaches . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The correct interaction of a costimulatory molecule such as CD40L with its contrareceptor CD 40 expressed on the membrane of professional APCs , provides transmembrane signaling that leads to APC activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Previously , we have reported that an Ad 5 vector targeted to CD 40 via genetic capsid incorporation of CD40L achieves selective transduction of DC in vitro . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Importantly , CHIR 12 . 12 abrogates CD40L induced growth and survival of CD 40 expressing patient multiple myeloma cells in the presence or absence of bone marrow stromal cells ( BMSC ) , without altering constitutive multiple myeloma cell proliferation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Inhibition of CD 40 CD40L interactions results in a reduction of innate regulatory T cells ( Tregs ) in CD 40 ( / ) mice and induces a stable plaque phenotype in atherosclerosis prone mouse strains . ^^^ As in CD 40 ( / ) mice , the CD45RB ( high ) : CD45RB ( low ) CD 4 T cell ratio significantly increased and the CD 25 ( + ) CD 4 ( + ) subpopulation significantly decreased in LDLR ( / ) mice receiving CD40L ( / ) BM compared to LDLR ( / ) mice receiving wild type BM . ^^^ In conclusion , the present study shows that CD 40 CD40L interactions on leukocytes are essential for the size of the CD45RB ( low ) CD 25 ( + ) CD 4 Treg subpopulation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
However , the 3T3 CD40L cell line , regularly used for engagement of CD 40 on the B cell surface , is a potential source of xenoantigens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We show that the ligand for CD 40 ( CD40L / CD154 ) is expressed in CD 40 positive human breast tumour biopsies , suggesting that the constitutive activation of the CD 40 receptor in vivo may contribute to the oncogenic process . ^^^ Coexpression of CD 40 and CD40L confers oncogenic effects on immortalized human epithelial cells in vitro , increasing their proliferation , motility and invasion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Persistence of high CD 40 and CD40L expression after restorative proctocolectomy for ulcerative colitis . ^^^ AIM : To focus on the role of CD 40 and CD40L in their pathogenesis . ^^^ METHODS : We analyzed by immunohistochemistry the CD 40 and CD40L expression in the pouch mucosa of 28 patients who had undergone RPC for UC , in the terminal ileum of 6 patients with UC and 11 healthy subjects . ^^^ RESULTS : Ileal pouch mucosa leukocytes presented a significantly higher expression of CD 40 and CD40L as compared to controls . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The aims of this study were to 1 ) quantify the constitutive and induced expression of CD 40 in different regional vascular beds of the mouse and 2 ) assess the role of CD40L as a modulator of vascular endothelial CD 40 expression . ^^^ Constitutive expression of CD 40 was profoundly reduced in unstimulated CD40L / mice , but the LPS induced CD 40 upregulation did not differ between CD40L / and WT mice . ^^^ Depletion of platelets or T lymphocytes , the major CD40L expressing cells in blood , also resulted in a profound reduction in basal CD 40 expression . ^^^ Platelet and T lymphocyte associated CD40L appears to play a major role in regulating the density of CD 40 expression on vascular endothelial cells in vivo . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : Cells in human atherosclerotic lesions express the immune mediator CD 40 and its ligand , CD40L , but the mechanisms and the mediators by which CD40L contributes to atherosclerosis are poorly defined . ^^^ Furthermore , inhibition of CD 40 expression by small interfering RNA blocked the effects of CD40L on O2 * , NO bioactivity , and PGIS nitration , which indicates a specific effect of CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Here we examine the effect of SLAM / SLAM interactions on CD40L induced CD 40 signaling pathways in human DCs . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Peripheral blood mononuclear cells were examined by FACS analysis for potential changes in specific cell populations ( T cells , B cells , monocytes ) , and for the expression of activation markers ( CD 25 , HLA DR ) , costimulatory molecules ( CD 40 , CD40L ) , TNF receptors , chemokine receptors , and adhesion molecules ( L selectin ( L sel ) ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Interaction between CD 40 , a member of the tumor necrosis factor receptor ( TNFR ) superfamily , and its ligand CD40L , a 39 kDa glycoprotein , is essential for the development of humoral and cellular immune responses . ^^^ These molecules interact with CD 40 , compete with the binding of CD40L to CD 40 , and reproduce , to a certain extent , the functional properties of the much larger homotrimeric soluble CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
There is a growing body of evidence that CD 40 CD40 ligand ( CD40L ) interaction also plays an important role in atherogenesis . ^^^ However , the effects of AGEs on CD 40 CD40L signaling in endothelial cells ( ECs ) remain to be elucidated . ^^^ In this study , we investigated ( 1 ) whether injection of AGE proteins to normal rats stimulates CD40L expression on circulating platelets and ( 2 ) whether AGEs up regulate CD 40 mRNA levels in cultured ECs . ^^^ These results suggest that AGEs could enhance CD 40 CD40L interaction , thereby promoting atherosclerosis in diabetes . ^^^ Blockade of CD 40 CD40L signaling in ECs may be a molecular target for the vasculoprotective property of nifedipine . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The monoclonal antibodies used included those to CD1a , CD 4 , CD 8 , CD 40 , CD40L , CD 68 , Fas , Fas ligand ( FasL ) and myeloperoxidase . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Constitutive expression of functional CD 40 on mouse renal cancer cells : induction of Fas and Fas mediated killing by CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
METHODS : In 38 patients with AMI , we investigated the effects of a thrombolytic regimen with half reteplase ( r PA ) dose plus abciximab vs . full dose r PA on membrane bound adhesion molecules ( CD 41 , CD42b , CD 40 , CD40L ) expressed on platelets , neutrophils and monocytes as well as on soluble platelet selectin as interaction and activation markers of these cells . ^^^ After 3 h , the percentage of CD 41 and CD42b positive monocytes and granulocytes as well as the percentage of CD 40 positive granulocytes and the percentage of CD40L positive monocytes markedly ( p < 0 . 01 , p < 0 . 05 ) decreased in the combination group vs . data at admission compared with the r PA group indicating less leukocyte patelet adhesion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In addition , DC overexpressing CD40L secreted considerably more IL 12 and expressed higher levels of the co stimulatory molecules , CD 80 , CD 86 and CD 40 , than did DC overexpressing LacZ , 4 1BBL or RANKL . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
RESULTS : OxLDL increased the expression of CD 40 and CD40L in a dose and time dependent manner . ^^^ Also , pretreatment of HUVECs with pioglitazone ( 1 and 10 micromol / l ) for 60 min also significantly decreased CD 40 and CD40L expression on HUVECs induced by oxLDL in a concentration dependent manner . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In the patients ' circulation , soluble CD40L ( sCD40L ) levels are elevated and surface CD40L is increased in platelets and T cells , whereas in the intestine CD 40 is overexpressed in the microvasculature and CD40L in platelets and T cells . ^^^ The therapeutic effects of infliximab in CD are attributed to its systemic anti TNF alpha action , but because TNF alpha modulates both CD 40 and CD40L , we investigated whether infliximab affects the CD40 / CD40L pathway in the intestine . ^^^ In vitro infliximab prevented TNF alpha induced CD 40 and VCAM 1 expression by HIMEC , and reduced PBT , but not platelet , surface CD40L expression and sCD40L release . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Recent studies suggest that CD 40 CD40 ligand ( CD40L ) system is proven to be an important mediator of several auto immune and chronic inflammation diseases . ^^^ In conclusion , our data provide evidence to support a direct pro inflammatory effect of CD 40 CD40L signaling pathway in CVECs , and lovastatin possesses an anti inflammatory effect independent of its lipid lowering action involved in the cerebrovascular diseases . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Significant inhibition of TNF alpha production was demonstrated when DC from peripheral blood mononuclear cells or cells eluted from intestinal mucosa of Crohn ' s disease were treated with either the HSP 70 inhibitory peptide ( aa 457 496 ) or peptides derived from CD 40 and CD40L . ^^^ These inhibitory peptides target the CD 40 CD40L and the emerging CD 40 HSP70 co stimulatory pathway . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Although these data suggest a potential role for CD40L in Alzheimer ' s disease pathology in transgenic mice they do not cast light on whether this effect is due to inhibition of signaling via CD 40 or whether it is due to the mitigation of some other unknown role of CD40L . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Variants of the CD 40 gene but not of the CD40L gene are associated with coronary artery calcification in the Diabetes Heart Study ( DHS ) . ^^^ METHODS : We investigated the role of genetic variation in CD 40 and CD40L genes in subclinical atherosclerosis assessed by coronary artery calcification ( CAC ) and carotid intima media thickness in 620 individuals from 230 families in the DHS . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
When stimulated with IL 3 and CD40L , pDCs of multiple sclerosis patients showed inefficient maturation as demonstrated by significantly lower or delayed upregulation of CD 86 , 4 1BBL , CD 40 and CD 83 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
FDCs express CD 40 , suggesting that T cell CD40L and lymphokines may be involved in regulating FDC CD 23 . ^^^ To test this , highly enriched FDCs were incubated with CD40L trimer or anti CD 40 to mimic T cell signaling in the presence of IFN gamma or IL 4 . ^^^ When FDCs were incubated with either CD40L trimer or agonistic anti CD 40 Ab , the expression of FDC CD 23 was increased both at the mRNA and protein levels . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Ligation of CD 40 by CD40L on T cells is critical for the induction of these immune responses in vivo . ^^^ We explored the role of CD40 / CD40L interactions in vaccine immunity to Blastomyces dermatitidis by immunizing CD 40 ( / ) and CD40L ( / ) mice and analyzing their resistance to reinfection in a murine pulmonary model . ^^^ In the absence of CD 40 or CD40L , CD 4 ( + ) cells failed to get primed or produce type 1 cytokine and impaired the generation of CD 8 ( + ) T 1 cells . ^^^ If CD 4 ( + ) cells were first eliminated , vaccination of CD 40 ( / ) and CD40L ( / ) mice restored priming of CD 8 ( + ) cells , type 1 cytokine production , and resistance . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Estradiol blocks the induction of CD 40 and CD40L expression on endothelial cells and prevents neutrophil adhesion : an ERalpha mediated pathway . ^^^ OBJECTIVE : Interferon gamma ( IFN gamma ) was shown to induce CD 40 and CD40L expression on endothelial cells ( ECs ) and consequently to promote neutrophil adhesion . ^^^ The pro and anti inflammatory effects of estrogens are well recognized but their role on the regulation of CD 40 and CD40L expression on ECs remains undefined . ^^^ METHODS AND RESULTS : Treatment of porcine aortic endothelial cells ( PAEC ) with IFN gamma for 24 h enhanced CD 40 and CD40L expression by 97 % and 78 % , respectively . ^^^ Treatment of PAEC with antisense oligomers targeting ERalpha mRNA attenuated the ability of 17betaE to inhibit the IFN gamma induced CD 40 and CD40L protein expression . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Mixed bone marrow chimera experiments reveal that , under homeostatic conditions , the CD40L CD 40 pathway can augment autoreactive B cell survival in a non cell autonomous manner . ^^^ Naive CD 4 T cells are shown to constitutively express CD40L mRNA and protein , although cell surface CD40L abundance is low because of engagement with CD 40 on other cells . ^^^ These observations indicate that the CD40L CD 40 pathway can augment the survival of autoantigen engaged B cells in the absence of T cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The increase in costimulation due to prolactin mediated up regulation of both CD 40 on B cells and CD40L on T cells would appear to play a central role in lupus induction in this model . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
BACKGROUND : High level production of heterodimeric p 70 interleukin ( IL ) 12 by myeloid derived dendritic cells ( DCs ) requires 2 signals : interferon gamma ( IFN gamma ) and a maturation signal provided by CD 40 ligation ( CD40L ) or lipopolysaccharide ( LPS ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 CD40L interaction activates extracellular signal regulated kinase 1 / 2 and nuclear factor kappaB pathways in insulinoma NIT 1 cells , and inhibitors of either pathway suppress cytokine / chemokine production in islets . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
OVA specific serum IgE was measured by ELISA , recruitment of inflammatory cells in BAL fluids and lung tissues by Diff Quik and H & E staining , respectively , the expressions of CD 40 , CD40L and VCAM 1 by immunohistochemistry , goblet cell hyperplasia by PAS staining , activities of MMPs by gelatin zymography , expressions of mRNA and proteins of cytokines by RT PCR and ELISA , activities of MAP kinases by western blot , and activity of NF KappaB by EMSA . ^^^ DA 9601 reduced IgE level , recruitment of inflammatory cells into the BAL fluid and lung tissues , expressions of CD 40 , CD40L and VCAM 1 molecules , goblet cell hyperplasia , MMPs activity , expressions of mRNA and productions of various cytokines , activities of MAP kinases and NK KappaB increased from OVA challenged mice . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The discovery of CD 40 and its ligand , gp 39 , have created new perspectives on the activation of resting B cells by TH cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 interactions are essential for germinal center formation and the development of B cell memory . gp 39 , the ligand for CD 40 expressed on activated CD4+ T helper cells , is required for the generation of antibody responses to T dependent ( TD ) antigens . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antibody to the ligand of CD 40 , gp 39 , blocks the occurrence of the acute and chronic forms of graft vs host disease . ^^^ In vivo administration of antibody to the ligand for CD 40 , gp 39 , blocked cGVHD induced serum anti DNA autoantibodies , IgE production , spontaneous immunoglobulin production in vitro , and associated splenomegaly . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The identification of the ligand for CD 40 , gp 39 , which is expressed on the membrane of activated CD4+ T helper cells , has sparked intense investigation into the roles of this molecule in physiological B cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The role of CD 40 and its ligand ( gp 39 ) in peripheral and central tolerance and its contribution to autoimmune disease . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Medium conditioned by human pancreatic carcinoma cells inhibited iMo DC proliferation , expression of costimulatory molecules ( CD 80 and CD 40 ) and of HLA DR , and functional activity as assessed by MLR and IL 12p70 production . iMo DC generated from pancreatic carcinoma patients in advanced stages of the disease similarly showed decreased levels of HLA DR expression and reduced ability to stimulate MLR in response to CD40L and IFN gamma . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The binding of a 39 kDa protein ( gp 39 ) , selectively expressed on activated Th cells , to its receptor CD 40 , on B cells , results in the initial B cell activation . ^^^
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Neither B 7 1 nor B 7 2 specific blocking mAb or soluble CTLA 4 , CD 40 or gp 39 restored the inhibition . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Stimulation of CD 40 with purified soluble gp 39 induces proinflammatory responses in human monocytes . ^^^ Engagement of CD 40 with anti CD 40 mAbs , gp 39 expressed on the cell surface or soluble forms of gp 39 , primes B cells to efficiently respond to subsequent stimulatory signals leading to B cell proliferation , differentiation , and isotype switching . ^^^ Using a soluble murine CD8 / human gp 39 fusion protein ( sgp 39 ) we have found that CD 40 plays a crucial role in the regulation of monocyte function . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The molecular basis for T cell help in humoral immunity : CD 40 and its ligand , gp 39 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Prevention of collagen induced arthritis with an antibody to gp 39 , the ligand for CD 40 . ^^^ The ligand for the CD 40 antigen is a 39 kilodalton protein , gp 39 , expressed on the surface of activated CD4+ T cells and is essential for thymus dependent humoral immunity . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Prolonged suppression of the humoral immune response by an antibody to the ligand for CD 40 , gp 39 . ^^^ The ligand for CD 40 has been recently identified as a 39 kd protein , gp 39 , expressed on the surface of activated CD4+ T helper cells ( Th ) . ^^^ In vitro , soluble CD 40 and anti gp 39 have been shown to block the ability of Th to activate B cells , suggesting that gp 39 CD40 interactions are important to T cell dependent B cell activation . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Blocking the interaction of T cell gp 39 , with its receptor / ligand on the surface of B cells ( CD 40 ) , completely blocked induction of CIA in mice . ^^^ A concomitant reduction in the level of anti CII IgG produced in anti gp 39 treated animals was observed , demonstrating the crucial importance of T cell : B cell interactions via gp 39 : CD 40 binding to the primary immune response to CII in vivo and therefore to the induction of CIA . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Following cleavage , there was no detectable binding of the monomeric form of CD 40 to a soluble form of gp 39 , the ligand of CD 40 , while the dimeric form was able to bind . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 gp 39 interactions play a critical role during allograft rejection . ^^^ In this report , we demonstrate that allograft rejection is also associated with strong induction of CD 40 and gp 39 transcripts . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
CD 40 , gp 39 , CD 70 and HSA . ^^^ The results suggest that potent co stimulatory pathways can be induced by cellular interactions between a T lymphoma , RMA S and T cells , not involving gp 39 , CD 40 , CD 70 , HSA , B 7 1 ( CD 80 ) or B 7 2 ( CD 86 ) . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Antibody to the ligand for CD 40 ( gp 39 ) inhibits murine AIDS associated splenomegaly , hypergammaglobulinemia , and immunodeficiency in disease susceptible C57BL / 6 mice . ^^^ These results support the possibility that molecular interactions between CD 40 and gp 39 are critical to the development of MAIDS . . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
T cell dependent activation of resting B cells involves the interaction of gp 39 on T cells with its receptor , CD 40 , on B cells . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
This interaction is a prerequisite , donating the two signals that are essential for IgE production : CD 40 ligation with its ligand gp 39 and exposure to interleukin ( IL ) 4 . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
We have used this method to generate functional sFv to human CD 2 , CD 3 , CD 4 , CD 8 , CD 28 , CD 40 , CD 45 and to murine CD 3 and gp 39 , from hybridomas producing murine , rat , or hamster antibodies . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
The function of gp 39 and its receptor CD 40 in thymus remains uncertain . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
There was a role for gp 39 : CD 40 co stimulation during antigen presentation to the T cells we studied , but the expression of CD 40 on T . cruzi infected macrophages was not decreased . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
In correlation with their selective effects on humoral and cellular immune responses , these chemokines also differentially attract CD 4 ( + ) versus CD 8 ( + ) T cells and modulate CD 40 , CD 80 , and CD 86 expressed by B 220 ( + ) cells as well as CD 28 , 4 1BB , and gp 39 expression by CD 4 ( + ) and CD 8 ( + ) T cells in a dose dependent fashion . ^^^
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
Taken together , these data demonstrate that T BAM on T cells and CD 40 on B cells are involved in contact dependent T B help interactions that occur in lymphoid follicles . . ^^^
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Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NA
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NA
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NA
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NA
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NA
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NA
Interacting proteins: P29965 and P25942 Pubmed SVM Score :0.0
NA