Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: Q8IVI9 and P29474 Pubmed SVM Score :0.77618622
Here , we show that NOSTRIN directly binds to caveolin 1 , a well established inhibitor of eNOS . 0.77618622^^^
Interacting proteins: Q8IVI9 and P29474 Pubmed SVM Score :0.0
In a search by the yeast two hybrid system using the eNOS oxygenase domain as bait , we have identified a previously uncharacterized eNOS interacting protein , dubbed NOSTRIN ( for eNOS traffic inducer ) . ^^^ Coimmunoprecipitation experiments demonstrated the eNOS NOSTRIN interaction in vitro and in vivo , and NOSTRIN ' s SH 3 domain was essential and sufficient for eNOS binding . ^^^ NOSTRIN colocalized extensively with eNOS at the plasma membrane of confluent human umbilical venous endothelial cells and in punctate cytosolic structures of CHO eNOS cells . ^^^ NOSTRIN overexpression induced a profound redistribution of eNOS from the plasma membrane to vesicle like structures matching the NOSTRIN pattern and at the same time led to a significant inhibition of NO release . ^^^ We conclude that NOSTRIN contributes to the intricate protein network controlling activity , trafficking , and targeting of eNOS . . ^^^
Interacting proteins: Q8IVI9 and P29474 Pubmed SVM Score :0.0
Homology search of all NCBI sequences indicated that the amino acid sequence of mDaIP 2 shares 82 % identity with human NOSTRIN which controls activity , trafficking , and targeting of nitric oxide synthase ( eNos ) . ^^^
Interacting proteins: Q8IVI9 and P29474 Pubmed SVM Score :0.0
METHODS : Western blot analysis was used to detect the expression of NOSTRIN and endothelial nitric oxide synthase ( eNOS ) . ^^^ A significant negative correlation existed between the expression of NOSTRIN protein and the activity of eNOS in the placental tissues of women with PE ( r= 0 . 57 , P < 0 . 01 ) . ^^^ CONCLUSION : The level of NOSTRIN expression in the placental tissues of women with PE is increased and is negatively correlated with the activity of eNOS which may play an important role in the pathogenesis of PE . . ^^^
Interacting proteins: Q8IVI9 and P29474 Pubmed SVM Score :0.0
The mDaIP 2 protein is a mouse orthologue of human Nostrin ( a regulator of eNos ) . ^^^ The absence of eNos activity in RA treated F 9 cell implies that the protein plays somehow different role from Nostrin . ^^^
Interacting proteins: Q8IVI9 and P29474 Pubmed SVM Score :0.0
NOSTRIN functions as a homotrimeric adaptor protein facilitating internalization of eNOS . ^^^ Recently , we described a novel eNOS interacting protein , NOSTRIN , which upon overexpression drives eNOS away from the plasma membrane towards intracellular compartments . ^^^ Accordingly , we show here that NOSTRIN interacts with the large GTPase dynamin and the actin nucleation promoting factor N WASP by means of its SH 3 domain , which also represents the docking site for eNOS . ^^^ Consistent with this notion , expression of dynamin 2 GFP in CHO cells stably expressing eNOS ( CHO eNOS ) results in recruitment of eNOS to dynamin positive structures , only when NOSTRIN is present as well . ^^^ Similarly , when N WASP GFP and NOSTRIN are co expressed in CHO eNOS cells , both proteins strongly co localize with eNOS and are recruited to structures running along actin filaments . ^^^