| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.62931825 |
| Because activity of endothelial NOS ( eNOS ) is thought to be regulated by its interaction with the caveolar protein caveolin 1 , structural relationships between eNOS , caveolin 1 , and the VEGF receptor FIk 1 / KDR were analyzed with double label immunofluorescence and cell fractionation procedures . 0.62931825^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.58945218 |
| Increased interaction of eNOS with caveolin 1 may deactivate the enzyme subsequent to its activation by Ca2+ / calmodulin . . 0.58945218^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.80326661 |
| To test this idea , we examined the interactions of eNOS with caveolin 1 in vitro and in vivo . 0.80326661^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.99131321 |
| In endothelial cells , eNOS interacts with caveolin 1 , which represses eNOS enzyme activity . 0.99131321^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.71905615 |
| Because eNOS activity is regulated by its interaction with the caveolar structural protein caveolin 1 , we analyzed VEGF effects on structural interactions between eNOS , caveolin 1 and the VEGF receptor Flk 1 / KDR . 0.71905615^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.75041376 |
| These immunolabeling patterns suggest functional interactions between eNOS and CAV 1 throughout the endothelium , regional differences in the modulation of nNOS by caveolin isoforms in vascular smooth muscle , and modulation of nNOS by CAV 3 in skeletal muscle . . 0.75041376^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.80567076 |
| Purified eNOS associates with GST Cav specifically through the caveolin scaffolding domain ( residues 82 101 ) ; however , the addition of CaM slightly , but nonstatistically , reduces eNOS binding to GST Cav . 0.80567076^^^ The association of eNOS with caveolin 1 ( Cav ) is believed to maintain eNOS in an inactive state ; however , increased association of eNOS to heat shock protein 90 ( hsp 90 ) is observed following activation . 0.61850744^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.84721534 |
| However , eNOS was tightly coupled with caveolin 1 , and was dissociated from heat shock protein 90 or calmodulin in the hypoxic pulmonary artery in either the presence or absence of carbachol . 0.84721534^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :1.6499747 |
| The astroglial NOS 3 like protein is apparently inactive , as reported for phosphorylated human NOS 3 associated with caveolin 1 . . 1.6499747^^^ Moreover , the astroglial NOS 3 like protein is constitutively tyrosine phosphorylated and associated with caveolin 1 . 1.2771035^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Immunogold labeling on samples of isolated perfused rat hearts embedded by an innovative low temperature LR White procedure provided detailed insight into the interaction of caveolin 1 and endothelial NOS in myocardial capillary endothelium at the subcellular level . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Recently , the scaffolding domain of caveolin 1 ( CAV ) has been implicated as a negative regulator of endothelial NOS ( eNOS ) . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Immunoblot and immunohistochemistry results indicate the following : 1 ) LAD endothelial NOS protein content was similar among groups ; 2 ) HF decreased LAD superoxide dismutase ( SOD ) but increased caveolin 1 content ; and 3 ) Ex increased SOD content of HF LADs . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Cardiac and aortic expression of endothelial NOS and caveolin 1 were measured by immunoblotting . ^^^ Aortic caveolin 1 protein , an inhibitor of endothelial NOS , was also increased in these mice by 2 . 0 fold and correlated positively with systolic BPV in the very low frequency band . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| To explore the participation of nitric oxide ( NO ) and caveolin 1 in this protective effect , we evaluated proteinuria , creatinine clearance , renal structural lesions , nitrites and nitrates urinary excretion ( UNO ( 2 ) ( ) / NO ( 3 ) 5 ) , and mRNA and protein levels of neuronal NO synthase ( nNOS ) , endothelial NOS ( eNOS ) , and caveolin 1 in lean and fatty Zucker rats fed with 20 % casein or soy protein diet . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Tissue levels of connexins 40 , 43 ( major components of gap junction ) , inducible NOS ( iNOS ) , endothelial NOS ( eNOS ) and eNOS regulator proteins such as calmodulin , heat shock protein 90 ( hsp 90 ) and caveolin 1 were also examined using Western blot . 2 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| LPS inhibits endothelin 1 induced endothelial NOS activation in hepatic sinusoidal cells through a negative feedback involving caveolin 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| N ( G ) nitro l arginine methyl ester ( L NAME , a non selective NOS inhibitor ) , N ( 6 ) ( 1 iminoethyl ) lysine ( NIL , an iNOS inhibitor ) , and 7 nitroindazole ( 7 NI , a nNOS inhibitor ) prevented the loss of caveolin 1 in the core and penumbra of the ischemic brain , whereas l N ( 5 ) ( 1 iminoethyl ) ornithine ( L NIO , an endothelial NOS inhibitor ) showed less effect than the other NOS inhibitors . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In endothelial cells , we found that eNOS is quantitatively immunoprecipitated by antibodies to caveolin 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Because eNOS is localized in plasmalemma caveolae , we examined if tyrosine phosphorylated eNOS interacts with caveolin 1 , the coat protein of caveolae . ^^^ Immunoprecipitation of eNOS from bovine lung microvascular endothelial cells resulted in the co precipitation of caveolin 1 . ^^^ Conversely , immunoprecipitation of caveolin 1 resulted in the co precipitation of tyrosine phosphorylated eNOS . ^^^ Thus , tyrosine phosphorylation is a novel regulatory mechanism for eNOS and caveolin 1 is the first eNOS associated protein . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Endothelial nitric oxide synthase ( eNOS ) and caveolin 1 are associated within endothelial plasmalemmal caveolae . ^^^ It is not known , however , whether eNOS and caveolin 1 interact directly or indirectly or whether the interaction affects eNOS activity . ^^^ To answer these questions , we have cloned the bovine caveolin 1 cDNA and have investigated the eNOS caveolin 1 interaction in an in vitro binding assay system using glutathione S transferase ( GST ) caveolin 1 fusion proteins and baculovirus expressed bovine eNOS . ^^^ Results obtained using both in vitro and in vivo protein interaction assays show that both N and C terminal cytosolic domains of caveolin 1 interact directly with the eNOS oxygenase domain . ^^^ Interaction of eNOS with GST caveolin 1 fusion proteins significantly inhibits enzyme catalytic activity . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Endothelial nitric oxide synthase ( eNOS ) is targeted to caveoli through interaction with caveolin 1 ( cav 1 ) . cav 1 binding to a consensus site in the eNOS oxygenase domain is proposed to antagonize calmodulin ( CaM ) binding and thereby inhibit eNOS nitric oxide ( NO ) synthesis . ^^^ To study the mechanism , we examined how cav 1 scaffolding domain peptide ( amino acids 82 101 ; cav 1P ) would affect NO synthesis , NADPH oxidation , cytochrome c reduction , and ferricyanide reduction by full length eNOS or its isolated oxygenase and reductase domains . ^^^ Immunoblotting showed that full length eNOS , eNOS oxygenase , and eNOS reductase all bound to an immobilized glutathione S transferase cav 1 fusion protein . ^^^ Thus , cav 1 interacts independently with both oxygenase and reductase domains of eNOS . ^^^ The reductase interaction occurs independent of a cav 1 binding motif , is CaM reversible , and is of sufficient affinity to match cav 1P inhibition of NO synthesis by full length eNOS . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| It has been shown previously that the endothelial nitric oxide synthase ( eNOS ) interacts reversibly with the plasmalemmal caveolae structural protein , caveolin 1 . ^^^ The eNOS caveolin 1 interaction inhibits eNOS catalytic activity . ^^^ Reversible and inhibitory membrane docking interactions of eNOS , therefore , are not restricted to those with caveolin 1 but also occur with the bradykinin B 2 receptor . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Consistent with a pivotal role of Na ( + ) / Ca ( 2+ ) exchange in Ca ( 2+ ) dependent activation of eNOS , an NCX protein was detected in caveolin rich membrane fractions containing both eNOS and caveolin 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The isolation of coronary microvessels from the left ventricle was associated with an enrichment of endothelial cell markers such as eNOS , von Willebrand factor , and caveolin 1 , an observation supported by the detection of up to 15 fold higher levels of eNOS mRNA in coronary microvessels relative to the larger arteries . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Additional post translational mechanisms regulate the activity of eNOS , including the interaction of eNOS with caveolin 1 , heat shock protein 90 ( Hsp 90 ) , or membrane phospholipids , as well as enzyme translocation and phosphorylation . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In separate rats from the same groups , we compared eNOS and caveolin 1 ( CAV 1 ) protein abundance in pial arterioles ( via immunofluorescence analyses ) . ^^^ The estrogen related changes in eNOS dependent vasodilating function appear to be related , in part , to a capacity for E ( 2 ) to increase eNOS protein expression and , in part , to an E ( 2 ) associated diminution in endothelial CAV 1 expression . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Agonist modulated targeting of the EDG 1 receptor to plasmalemmal caveolae . eNOS activation by sphingosine 1 phosphate and the role of caveolin 1 in sphingolipid signal transduction . ^^^ Overexpression of transfected caveolin 1 cDNA together with EDG 1 and eNOS markedly diminished S1P mediated eNOS activation ; caveolin overexpression also attenuated agonist induced phosphorylation of EDG 1 receptor by > 90 % . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| These data imply that the caveolin 1 scaffolding domain can selectively regulate signal transduction to eNOS in endothelial cells and that small molecule mimicry of this domain may provide a new therapeutic approach . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Increased uptake of cholesterol by endothelial cells ( ECs ) upregulates the abundance of the structural protein caveolin 1 and impairs NO release through the stabilization of the inhibitory heterocomplex between caveolin 1 and endothelial NO synthase ( eNOS ) . ^^^ This was paralleled by a decreased inhibitory interaction between caveolin 1 and eNOS and a restoration and / or potentiation of the basal ( +45 % ) and agonist stimulated ( +107 % ) eNOS activity . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation studies demonstrated the specific interaction of eNOS and NOSIP in vitro and in vivo , and complex formation was inhibited by a synthetic peptide of the caveolin 1 scaffolding domain . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Here , we created a caveolin 1 null ( CAV 1 / ) mouse model , using standard homologous recombination techniques , to assess the role of caveolin 1 in caveolae biogenesis , endocytosis , cell proliferation , and endothelial nitric oxide synthase ( eNOS ) signaling . ^^^ Our results indicate that eNOS activity is up regulated in Cav 1 null animals , and this activity can be blunted by using a specific NOS inhibitor , nitro l arginine methyl ester . ^^^ These findings are in accordance with previous in vitro studies showing that caveolin 1 is an endogenous inhibitor of eNOS . ^^^ Thus , caveolin 1 expression is required to stabilize the caveolin 2 protein product , to mediate the caveolar endocytosis of specific ligands , to negatively regulate the proliferation of certain cell types , and to provide tonic inhibition of eNOS activity in endothelial cells . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Some consideration is given to recent findings that suggest that estrogen can stimulate eNOS activity by decreasing the expression of the eNOS inhibitor caveolin 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The marked impairment in cerebrovascular endothelial nitric oxide synthase ( eNOS ) function that develops after ovariectomy may relate to the observation that the abundance of cerebral vascular eNOS and its endogenous inhibitor , caveolin 1 , vary in opposite directions with chronic changes in estrogen status . ^^^ The authors endeavored , therefore , to establish a link between these correlative findings by independently manipulating , in ovariectomized female rats , eNOS and caveolin 1 expression , while monitoring agonist ( acetylcholine ) stimulated eNOS functional activity . ^^^ In the current study , the authors showed that individually neither the up regulation of eNOS ( through simvastatin treatment ) , nor the down regulation of caveolin 1 ( through antisense oligonucleotide administration ) is capable of restoring eNOS function in pial arterioles in vivo in these estrogen depleted rats . ^^^ Only when eNOS up regulation and caveolin 1 down regulation are combined is activity normalized . ^^^ These results establish a mechanistic link between the estrogen associated divergent changes in the abundance of caveolin 1 and eNOS protein and eNOS functional activity in cerebral arterioles . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Endothelial nitric oxide synthase ( eNOS ) is regulated both by caveolin 1 and 17beta estradiol ( E ( 2 ) ) . ^^^ Both mRNA and protein for caveolin 1 were increased significantly only after 48 h treatment with E ( 2 ) , but eNOS protein and NO ( 10 ) production were decreased compared with cells treated for 24 h . ^^^ The results further suggest that estrogen may indirectly regulate NO ( 10 ) through caveolin 1 expression , which inhibits eNOS catalytic activity . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| TCV 116 stimulates eNOS and caveolin 1 expression and improves coronary microvascular remodeling in normotensive and angiotensin 2 induced hypertensive rats . ^^^ This study investigated the effects of an Ang 2 type 1 receptor antagonist , TCV 116 , on endothelial nitric oxide synthase ( eNOS ) mRNA and protein expression , and NOS activity and eNOS regulatory protein caveolin 1 protein expression in the left ventricle of Wistar Kyoto rats treated for 2 weeks with Ang 2 ( 200 ng / kg / min ) and evaluated these relations to myocardial remodeling . ^^^ The eNOS mRNA and protein levels , and NOS activity and caveolin 1 protein expression in the left ventricle were significantly decreased in ANGII compared with control rats ( CON ) , and were significantly increased in ANGII TCV compared with ANGII . ^^^ Moreover , compared with CON , the eNOS and caveolin 1 expression was significantly greater in CON treated with TCV 116 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In these cells , expression of caveolin 1 ( cav 1 ) stimulates caveolae biogenesis , promotes the interaction of cav 1 with eNOS , and the inhibition of NO release from cells . ^^^ Interestingly , in cells where cav 1 does not drive caveolae assembly , despite equal levels of cav 1 and eNOS and localization of both proteins to raft domains of the plasmalemma , the physical interaction of eNOS with cav 1 is dramatically less resulting in less inhibition of NO release . ^^^ Thus , cav 1 concentrated in caveolae , not in rafts , is in closer proximity to eNOS and is necessary for negative regulation of eNOS function , thereby providing the first clear example of spatial regulation of signaling in this organelle that is distinct from raft domains . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 , 90 kDa heat shock protein , ERK 1 / 2 , and Akt , all known and proposed regulators of eNOS activity , were detected throughout the ovine adrenal cortex . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Estrogen associated repression of inflammation may be due to upregulation of the endothelial isoform of nitric oxide synthase ( eNOS ) and concomitant downregulation of the endogenous inhibitor of eNOS , caveolin 1 ( CAV 1 ) . ^^^ In this study we examined the effects of estrogen independent eNOS upregulation ( via simvastatin ) and / or CAV 1 downregulation ( antisense ) on pial venular leukocyte adhesion in ovariectomized ( OVX ) rats . ^^^ These data also suggest that simvastatin induced upregulation of eNOS expression in OVX rats will not restore eNOS function , as measured by decreased leukocyte adhesion , unless CAV 1 levels are reduced as well . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 is a member of a subset of intracellular proteins that regulate endothelial nitric oxide synthase ( eNOS ) activity . ^^^ In caveolae , caveolin 1 inhibits eNOS activity via a direct interaction with the enzyme . ^^^ Previous work has indicated that both eNOS and caveolin 1 are also localized at the perinuclear Golgi complex . ^^^ Whether caveolin 1 is involved in eNOS regulation in this cell compartment is unknown . ^^^ Here we studied the localization of eNOS and caveolin 1 in the perinuclear region of primary bovine aortic endothelial cells . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The aim of the present study was to examine the localizations of eNOS and caveolin 1 at protein level in normal human liver tissue , and how the expressions are altered in cirrhotic liver . ^^^ RESULTS : Immunohistochemistry revealed that both eNOS and caveolin 1 were sparsely expressed on hepatic sinusoidal lining in normal liver specimens , and these findings were confirmed by Western blot . ^^^ Both immunohistochemistry and Western blotting demonstrated over expression of eNOS and caveolin 1 in cirrhotic liver specimens . ^^^ Morphometric analysis of immunogold particle labeling for eNOS and caveolin 1 was performed on immunoelectron micrographs . ^^^ In normal liver tissue , hepatic stellate cells and sinusoidal endothelial cells ( SEC ) expressed low levels of caveolin 1 , and SEC expressed a very low level of eNOS . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 coimmunoprecipitates with eNOS ; interaction with eNOS occurs via the caveolin 1 scaffolding domain and appears to result in the inhibition of NOS activity . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| One hypothesis is n LDL increases caveolin 1 ( Cav 1 ) , which decreases nitric oxide ( * NO ) production by binding endothelial nitric oxide synthase ( eNOS ) in an inactive state . ^^^ To test these hypotheses , EC were incubated with n LDL and then analyzed for * NO , O ( 2 ) ( * ) , phospho eNOS ( S 1179 ) , eNOS , Cav 1 , calmodulin ( CaM ) , and heat shock protein 90 ( hsp 90 ) . n LDL increased NOx by more than 4 fold while having little effect on A 23187 stimulated nitrite production . ^^^ In contrast , n LDL decreased cGMP under basal and A 23187 stimulated conditions and increased O ( 2 ) ( * ) by a mechanism that could be inhibited by L nitroargininemethylester ( L NAME ) and BAPTA / AM . n LDL increased phospho eNOS by 149 % , eNOS by approximately 34 % , and Cav 1 by 28 % , and decreased the association of hsp 90 with eNOS by 49 % . n LDL did not appear to alter eNOS distribution between membrane fractions ( approximately 85 % ) and cytosol ( approximately 15 % ) . ^^^ Only 3 6 % of eNOS in membrane fractions was associated with Cav 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Endothelial nitric oxide ( NO ) synthase ( eNOS ) is controlled by Ca ( 2+ ) / calmodulin and caveolin 1 in caveolae . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Because loss of caveolin 1 expression results in constitutive activation of eNOS activity , we also examined whether these increases in microvascular permeability are NO dependent . ^^^ Thus , caveolin 1 plays a dual regulatory role in controlling microvascular permeability : ( 1 ) as a structural protein that is required for caveolae formation and caveolar transcytosis and ( 2 ) as a tonic inhibitor of eNOS activity to negatively regulate the paracellular pathway . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Studies in cultured cells show that activation of endothelial nitric oxide ( NO ) synthase ( eNOS ) requires the dissociation of this enzyme from its inhibitory association with caveolin 1 ( Cav 1 ) , and perhaps its translocation from plasma membrane caveolae to other cellular compartments . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Translocation of the eNOS from the plasma membrane , where it is bound to caveolin 1 , to the cytosol is the crucial step in the synthesis of NO . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The recent colocalization of the cationic amino acid transporter CAT 1 ( system y ( + ) ) , nitric oxide synthase ( eNOS ) , and caveolin 1 in endothelial plasmalemmal caveolae provides a novel mechanism for the regulation of NO production by L arginine delivery and circulating hormones such insulin and 17beta estradiol . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The translocation of the enzyme from the plasma membrane , where it is located in caveolae bound to caveolin 1 , to the cytosol is the crucial step for the synthesis of NO through the eNOS isoform . ^^^ We demonstrate that bFGF activates a sphingomyelinase to synthesize ceramide , which , in turn , allows the dissociation of eNOS from caveolin 1 and its translocation to the cytosol in the active form , where it catalyzes the synthesis of NO . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In LV tissue of SHR at 18 weeks , caveolin 1 and 3 were similarly decreased , but Hsp 90 upregulated , together with a downregulation of eNOS . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| We found that the protein contents of eNOS , actin , and caveolin 1 were significantly higher in the caveolar fraction of plasma membranes than in the noncaveolar fraction of plasma membranes in PAEC . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Estrogen receptors ( ERalpha and ERbeta ) , heat shock proteins ( hsp 70 and hsp 90 ) , endothelial nitric oxide synthases ( eNOS ) , caveolin 1 , 2 and 3 and calmodulin were analyzed in total platelet lysate by immunoblotting . ^^^ Expression of ERalpha and ERbeta , hsp 70 , hsp 90 , eNOS , calmodulin , and caveolin 1 were observed in both sexes . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Laminar shear stress applied to static endothelial cultures ( flow step of 5 dyn / cm2 ) , enhanced the tyrosine phosphorylation of luminal surface proteins by 1 . 7 fold , including caveolin 1 by 1 . 3 fold , increased Ser 1179 phosphorylation of endothelial nitric oxide synthase ( eNOS ) by 2 . 6 fold , and induced a 1 . 4 fold activation of mitogen activated protein kinases ( ERK1 / 2 ) over no flow controls . ^^^ The same shear step applied to endothelial cells preconditioned under 10 dyn / cm2 of laminar shear stress for 6 h and induced a sevenfold increase of total phosphotyrosine signal at the luminal endothelial cell surface enhanced caveolin 1 tyrosine phosphorylation 5 . 8 fold and eNOS phosphorylation by 3 . 3 fold over static control values . ^^^ In addition , phosphorylated caveolin 1 and eNOS proteins were preferentially localized to caveolar microdomains . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| A major structural component is the membrane protein caveolin 1 which associates with numerous signalling molecules , including endothelial nitric oxide ( eNOS ) . ^^^ Caveolin 1 , which co immunoprecipitates with eNOS in preparations from endothelial cells , regulates eNOS activity , holding it inactive . ^^^ Controversy now exists regarding the presence of caveolae and caveolin 1 in trophoblasts , hence this study was carried out to examine whether the high levels of eNOS expressed in human syncytiotrophoblast are associated with caveolin 1 , and to find out if caveolae are present in villous cytotrophoblasts and syncytiotrophoblast . ^^^ The time course of expression of caveolin 1 and eNOS during differentiation of villous cytotrophoblast into syncytiotrophoblast in culture was studied . ^^^ Western analysis showed that caveolin 1 expression evident in day 1 whole cell lysates decreased at day 3 when the cells had syncytialized and declined further by day 6 , while the levels of actin ( control ) remained high . eNOS expression in the same samples followed a different pattern , with the low levels in day 1 cells increasing substantially by 3 days in culture , subsiding again by day 6 . eNOS association with caveolin 1 in day 1 and day 3 trophoblast cultures was evidenced by the demonstration that eNOS co immunoprecipitates with caveolin 1 and vice versa . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| This effect involves the transcriptional activation of the gene encoding caveolin 1 , a structural protein of caveolae that acts as a negative allosteric regulator of eNOS . ^^^ Treatment of endothelial cells with statins ( inhibitors of cholesterol synthesis ) abrogates caveolin 1 upregulation and restores eNOS activity in vitro and in vivo in genetically apoE deficient , hypercholesterolemic mice . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The decrease in activity occurred within 1 hour of drug treatment and was not accompanied by a change in intracellular levels of either eNOS or its inhibitor caveolin 1 . ^^^ Taken together , these data may indicate that eNOS is regulated by an interacting protein , different from caveolin 1 , that inhibits its activity and is rapidly degraded by the proteasome in the presence of eNOS agonists . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| METHODS : Anti eNOS , anti caveolin 1 , and anti calmodulin antibodies were used for Western blotting . ^^^ For in situ hybridization ( ISH ) , human eNOS and caveolin 1 peptide nucleic acid probes were used with a catalyzed signal amplification system . ^^^ By ISH , eNOS mRNA was localized on portal vein and hepatic lining cells , and caveolin 1 mRNA was almost undetectable in normal liver tissue . ^^^ In cirrhotic liver tissue , caveolin 1 mRNA was overexpressed on hepatic sinusoidal lining cells , while eNOS mRNA expression was similar to that in normal liver . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In the endothelium , caveolin 1 regulates nitric oxide signaling by binding to and inhibiting endothelial nitric oxide synthase ( eNOS ) . ^^^ Increased cytosolic Ca2+ or activation of the kinase Akt leads to eNOS activation and its dissociation from caveolin 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Human OECs , but not EPCs , expressed key regulatory proteins endothelial nitric oxide synthase ( eNOS ) and caveolin 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Our recent immunoelectron microscopic and Western blot studies have revealed that caveolin 1 , i . e . the principal structural protein of caveolae , and endothelial nitric oxide synthase ( eNOS ) co exist in the plasma membrane of the SEF , implying that the SEF may correspond to a permanent ( stationary ) type of fused and interconnected caveolae , thus contributing to the local control of hepatic sinusoidal blood flow by the regulation of NO synthesis . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In cultured ECs , a high level of LDL increased the abundance of eNOS and caveolin 1 ( Cav 1 ) in the membrane caveolae and the association of eNOS with Cav 1 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Because of the facilitatory effects of statins on endothelial function and the adverse effects of rapamycin ( RAPA ) on plasma lipids , we compared the effects of simvastatin ( SMV ) and RAPA on endothelial NO synthase ( eNOS ) and Cav 1 protein expression and phosphorylation in the aortas of apolipoprotein E ( Apo E ) knockout ( / ) mice . ^^^ The increase in eNOS induced by RAPA and the inverse relationship between p eNOS and Cav 1 protein expression observed with SMV treatment suggest different mechanisms for the regulation of aortic eNOS expression in Apo E mice by these 2 agents . . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Like primary HUVEC cells , HUVEC CS express many of the key proteins necessary for vasodilator production , including epithelial nitric oxide synthase ( eNOS ) , HSP 90 , cav 1 and 2 , cPLA 2 , and COX 1 and 2 . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| However , CsA treatment decreased cholesterol content in caveolae and displaced eNOS from caveolae , which may be caused by CsA disrupting the association of caveolin 1 and cyclophilin A . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Basal eNOS activity is also regulated by interaction with caveolin 1 , the major coat protein of caveolae . ^^^ In the present study we have examined in rat aorta endothelium the subcellular steady state distribution of eNOS , the catalytic subunit of PKA ( PKA c ) , and caveolin 1 . ^^^ PKA c colocalized with eNOS in the lamellipodia , whereas caveolin 1 was absent from these membrane domains . ^^^ |
|
| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In endothelia , NO is synthesized by endothelial NO synthase ( eNOS ) , which is negatively regulated by caveolin 1 ( Cav 1 ) , the primary coat protein of caveolae . ^^^ To characterize the molecular mechanism by which the AP Cav peptide and Cav 1 mediate eNOS inhibition , we subdivided the Cav portion of AP Cav into three domains ( Cav A , B , and C ) , synthesized five overlapping peptides ( AP Cav A , AB , B , BC , and C ) , and tested their effects on eNOS dependent activities . ^^^ Peptides containing the Cav B domain ( amino acids 89 95 ) induced time and dose dependent inhibition of eNOS dependent NO release in cultured endothelial cells , NO dependent inflammation in the ear , and hydraulic conductivity in isolated venules . ^^^ Alanine scanning of AP Cav B revealed that Thr 90 and 91 ( T 90 , 91 ) and Phe 92 ( F 92 ) are crucial for AP Cav B and AP Cav mediated inhibition of eNOS . ^^^ Mutation of F 92 to A 92 in the Cav 1 cDNA caused the loss of eNOS inhibitory activity compared with wild type Cav 1 . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Endothelial nitric oxide synthase ( eNOS ) , the major nitric oxide ( NO ) generating enzyme of the vasculature , is regulated through multiple interactions with proteins , including caveolin 1 , Hsp 90 , Ca2+ calmodulin , and the recently discovered eNOS interacting protein , NOSIP . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Furthermore , eNOS enzymic activity is increased in lysates of CAT 1 overexpressing cells accompanied by increased phosphorylation of eNOS at Ser 1179 and Ser 635 , and decreased association of eNOS with caveolin 1 . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Expression of NADPH oxidase , Akt , pAkt , Bcl 2 , Bax , IkappaB , caveolin 1 and eNOS was evaluated by Western blot analysis . 2 ET 1 significantly enhanced ROS generation and cell proliferation following 24 h incubation , both of which were prevented by BQ 788 or apocynin , consistent with the ability of ET 1 to directly upregulate NADPH oxidase . ^^^ Furthermore , ET 1 downregulated expression of caveolin 1 and eNOS , which was attenuated by BQ 788 or apocynin . 3 In summary , our results suggest that ET 1 affects oxidative stress , proliferation and apoptosis possibly through ET ( B ) , NADPH oxidase , Akt , Bax and caveolin 1 mediated mechanisms . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The activity of endothelial nitric oxide synthase ( eNOS ) , and the expression levels of caveolin 1 and intercellular adhesion molecule 1 ( ICAM 1 ) were determined by Western blotting . ^^^ Functional analysis demonstrated that the YCPRYVRRKLENELLVL peptide shared by two clones inhibited the expression of ICAM 1 , increased nitric oxide concentration in the culture media , and upregulated the expression of caveolin 1 and eNOS . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| We measured the levels of eNOS by ELISA and its binding proteins including heat shock protein 90 ( Hsp 90 ) and caveolin 1 by Western blotting . ^^^ Neither Hsp 90 nor caveolin 1 important eNOS regulators appears to mediate the genotypesmoking effects on eNOS expression although HUVECs did produce more Hsp 90 when exposed to CSE . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation demonstrated an association between TbetaRI and TbetaRII , as well as an association of the TbetaRs receptors with Cav 1 and eNOS ( endothelial nitric oxide synthase ) , suggesting a mutual co localization to caveolae ; after treatment of HUVEC with 5 ng / ml TGF beta 1 for 15 min , however , co precipitation of eNOS with TbetaRI , TbetaRII and Cav 1 was diminished . ^^^ The loss of immunoprecipitable eNOS from Cav 1 enriched fractions was accompanied by a decrease both in phosphorylation of eNOS and in enzymatic activity ( conversion of arginine into citrulline ) . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Effect of chronic ethanol administration on hepatic eNOS activity and its association with caveolin 1 and calmodulin in female rats . ^^^ We then tested the hypothesis that an imbalance between the binding of eNOS with inhibitory and stimulatory proteins may underlie the reduced activity of eNOS because eNOS catalytic activity is regulated partly through dynamic interactions with the inhibitory protein caveolin 1 and the stimulatory protein calmodulin . ^^^ The binding of caveolin 1 and calmodulin with eNOS was increased and decreased , respectively , in alcohol treated rats . ^^^ Our results suggest that chronic alcohol intake attenuates hepatic eNOS activity by increasing the expression of the inhibitory protein caveolin 1 and enhancing its binding with eNOS . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The increased expression and phosphorylation of eNOS with RPM appears to be regulated by mechanisms other than Akt or Caveolin 1 . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| However , in the last five years it is clear that eNOS activity and NO release can be regulated by post translational control mechanisms ( fatty acid modification and phosphorylation ) and protein protein interactions ( with caveolin 1 and heat shock protein 90 ) that direct impinge upon the duration and magnitude of NO release . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Phytoestrogen genistein supplementation increases eNOS and decreases caveolin 1 expression in ovariectomized rat hearts . ^^^ Protein level of eNOS , caveolin 1 and calmodulin was determined by Western blot . ^^^ Administration of genistein also increased eNOS protein expression in OVX rats myocardium with a concomitant decrease in the expression of caveolin 1 , an endogenous eNOS inhibitory protein . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| In this study , caveolin 1 ( cav 1 ) , an inhibitor of endothelial nitric oxide synthase ( eNOS ) , was semi quantified in diseased human and rabbit blood vessels . ^^^ Excess segments of human internal mammary arteries ( IMA ) and radial arteries ( RA ) were obtained from patients undergoing coronary artery bypass surgery . eNOS and cav 1 were localized throughout both human and rabbit vessels . ^^^ Interestingly , the endothelial cav 1 : eNOS ratio increased 5 fold only in endothelium overlying plaques but decreased in endothelium overlying vessels with neo intimal thickening . ^^^ In human tissue , there was no difference between RA and IMA eNOS immunoreactivity in endothelium , intima , or media ; however , RA endothelial , intimal , and medial cav 1 immunoreactivity increased 4 fold ( p < 0 . 02 ) , 8 fold ( p < 0 . 001 ) , and 4 fold ( p < 0 . 004 ) , respectively , compared with IMA . ^^^ Furthermore , the cav 1 : eNOS immunostaining ratio in the media correlated with intimal thickening ( r 2 = 0 . 5 ) . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| METHODS : Changes in caveolin 1 , calmodulin , and eNOS expression were determined by western blot and densitometric analysis . ^^^ Endothelin receptor expression and localization and the intracellular localization of eNOS and caveolin 1 were assessed by confocal microscopy . ^^^ RESULTS : Sinusoidal endothelial cells expressed caveolin 1 and calmodulin , and expression was altered in cultured and passaged cells . eNOS expression decreased significantly in 24 h cultured cells , with expression dropping below the level of detection in passaged cells . ^^^ In 24 h cultured cells , caveolin 1 was localized in the perinuclear region and cell membrane , while eNOS was predominantly localized in the perinuclear region , where it co localized with caveolin 1 . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Glimepiride activates eNOS with a mechanism Akt but not caveolin 1 dependent . ^^^ Insulin stimulates caveolin 1 and eNOS phosphorylation . ^^^ We found that glimepiride induces caveolin 1 and eNOS phosphorylation . ^^^ Caveolin 1 silencing did not change eNOS and Akt phosphorylation induced by glimepiride . ^^^ These findings suggest that glimepiride induces eNOS phosphorylation in endothelial cells through an Akt dependent mechanism , not regulated by caveolin 1 . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| We revealed that this beneficial effect was not dependent on the increase in eNOS mRNA or protein expression , but was dependent on the inhibition of the eNOS tight coupling with caveolin 1 , the eNOS dissociation from heat shock protein 90 , and the decrease in eNOS Ser 1177 phosphorylation induced by hypoxia . ^^^ Furthermore , in a whole mount immunostaining the hypoxia induced eNOS protein condensation with caveolin 1 of pulmonary endothelial cells was restored by the fluvastatin treatment . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Endothelial NO synthase ( eNOS ) activity is determined by heat shock protein 90 ( HSP 90 ) , caveolin 1 , and the cofactor tetrahydrobiopterin ( BH 4 ) . ^^^ Expression of eNOS , HSP 90 , caveolin 1 , Akt , phosphorylated eNOS ( eNOS Ser 1177 ) , and GTPCH 1 were determined by Western blot analysis . ^^^ Expression of eNOS , caveolin 1 , phosphorylated Akt , and eNOS Ser 1177 was enhanced in aortas exposed to increased blood flow . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Insulin and IGF 1 phosphorylate eNOS in HUVECs by a caveolin 1 dependent mechanism . ^^^ Also eNOS is targeted to caveolae and caveolin 1 , the major caveolar protein , acts as a regulator of eNOS activity . ^^^ Since Insulin and IGF 1 phosphorylate and activate eNOS , we investigated the role of caveolin 1 in Insulin and IGF 1 stimulated eNOS activity . ^^^ Here we show that : ( 1 ) in human endothelial cells , Insulin and IGF 1 stimulate eNOS phosphorylation in a different manner both qualitatively and quantitatively ; ( 2 ) caveolin 1 down regulation abolishes Insulin and IGF 1 stimulated eNOS phosphorylation . ^^^ These results suggest that caveolae could represent an intracellular site that contributes to differentiate IR and IGF IR activity , and demonstrate the role of caveolin 1 in the eNOS activation by Insulin and IGF I . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 , an eNOS inhibitory protein , was upregulated after LPS and chronic alcohol consumption . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Acute OS increased the monomeric form of the inhibitory protein caveolin 1 ( 1 . 2 + / 0 . 05 vs 0 . 9 + / 0 . 02 , p < 0 . 01 ) and increased the eNOS caveolin association as determined by coimmunoprecipitation ( 1 . 24 + / 0 . 04 vs 0 . 97 + / 0 . 04 , p < 0 . 05 ) . ^^^ Our findings indicate that acute and subacute oxidative stress result in the inhibition of induced nitric oxide synthase activity through distinct mechanisms dependent on caveolin 1 inhibition , ET ( B ) dissociation , and eNOS phosphorylation . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| As expected there was only a weak colocalization between eNOS phosphorylated at Ser 1177 and caveolin 1 . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| This was accompanied by significant glomerulosclerosis , tubulointerstitial damage , renal immune cell infiltration , marked down regulations of renal tissue eNOS and nNOS , mild reduction of caveolin 1 , and unchanged calmodulin , phospho AKt , and sGC . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Multiple signaling inputs , including calcium , caveolin 1 , phosphorylation by several kinases , and binding to the 90 kDa heat shock protein ( Hsp 90 ) , regulate eNOS activity . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| It is widely believed that at the cell surface eNOS is localized in caveolae , where caveolin 1 negatively regulates its activity , however , there are still uncertainties on its intracellular distribution . ^^^ In confluent and non confluent HUVEC and HeLa cells , we failed to detect substantial colocalization between eNOS and caveolin 1 at the cell surface . ^^^ Instead , in non confluent cells , eNOS was concentrated in ruffles and at the leading edge of migrating cells , colocalizing with actin filaments and with the raft marker ganglioside G ( M 1 ) , and well segregated from caveolin 1 , which was restricted to the posterior region of the cells . ^^^ Our results provide a morphological correlate for the role of eNOS in cell migration and raise questions on the site of interaction between eNOS and caveolin 1 . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The samples were used for protein expression profiling by the Ciphergen Proteinchip system and immunoblotting analysis of endothelial nitric oxide synthase ( NOS 3 , also termed eNOS ) and caveolin 1 . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Together with previous experiments examining the interaction between CSD and endothelial nitric oxide synthase ( eNOS ) , the results suggest a general oligomerization dependent enhancement of binding between signal transducing enzymes and caveolin 1 . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Concomitantly , caveolin 1 , an established down regulator of eNOS , was up regulated . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Changes in vascular expression of eNOS , caveolin 1 and p 47 were analysed by Western blot , eNOS activity by conversion of [ H ] arginine to L [ H ] citrulline , and NADPH oxidase activity by NADPH enhanced chemoluminescence of lucigenin . ^^^ Compared to WKY , SHR showed upregulated eNOS and p 47 protein expression , downregulated caveolin 1 expression , increased NADPH induced superoxide production but , paradoxically , eNOS activity was reduced . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| This suggests that other genes such as CAV 1 , whose product ( CAV 1 ) regulates eNOS activity , could also be related to this phenotype . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| We tested in vivo the hypotheses that : ( a ) endothelial nitric oxide synthase ( eNOS ) is not essential for regulation of baseline permeability ; ( b ) eNOS is essential for hyperpermeability responses in inflammation ; and ( c ) molecular inhibition of eNOS with caveolin 1 scaffolding domain ( AP Cav ) reduces eNOS regulated hyperpermeability . ^^^ We conclude that : ( 1 ) baseline permeability does not depend on eNOS ; ( 2 ) eNOS and NO are integral elements of the signalling pathway for the hyperpermeability response to PAF ; ( 3 ) iNOS does not affect either baseline permeability or hyperpermeability responses to PAF ; and ( 4 ) caveolin 1 inhibits eNOS regulation of microvascular permeability in vivo . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of eNOS and expression of calmodulin increased , but Hsp 90 decreased with all treatments and only raloxifene treatment increased caveolin 1 compared with OVX . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| The expression of caveolin 1 and the related molecule endothelial nitric oxide synthase ( eNOS ) was analyzed in the sciatic nerves of Lewis rats with experimental autoimmune neuritis ( EAN ) . ^^^ The pattern of eNOS expression over the course of EAN largely matched that of caveolin 1 . ^^^ Consequently , we postulated that caveolin 1 expression increased in the sciatic nerves with EAN ; this possibly mediated either molecular trafficking or nitric oxide generation partly through the activation of eNOS in vascular endothelial cells , as well as in inflammatory macrophages in EAN and / or cellular apoptosis of inflammatory cells . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Here , we show that NOSTRIN directly binds to caveolin 1 , a well established inhibitor of eNOS . ^^^ Consistently , we were able to demonstrate the existence of a ternary complex of NOSTRIN , eNOS , and caveolin 1 in Chinese hamster ovary ( CHO ) eNOS cells . ^^^ We propose that a ternary complex between NOSTRIN , caveolin 1 , and eNOS mediates translocation of eNOS , with important implications for the activity and availability of eNOS in the cell . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Protein expression of endothelial nitric oxide synthase ( eNOS ) , inducible NOS ( iNOS ) , caveolin 1 and PKC were determined . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Since one of the most characterized enzymes regulated by CAV 1 is eNOS , we decided to include both genes in this study . ^^^ Two of these SNPs were located within eNOS and three within the CAV 1 gene . ^^^ If both CG ( eNOS ) and TiA ( CAV 1 ) haplotypes were taken together , this association increased in significance . ^^^ Thus , we propose that CAV 1 , either alone or together with eNOS alleles , might modify CRC heritability . . ^^^ Genetic analysis of caveolin 1 and eNOS genes in colorectal cancer . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Cav 1 ( / ) mouse lungs demonstrated a significant increase in endothelial NO synthase ( eNOS ) derived NO production relative to WT , which is consistent with the role of caveolin 1 as a negative regulator of eNOS activity . ^^^ Cav 1 ( / ) mouse lungs demonstrated a significant increase in endothelial NO synthase ( eNOS ) derived NO production relative to WT , which is consistent with the role of caveolin 1 as a negative regulator of eNOS activity . ^^^ Thus , caveolin 1 , through its ability to regulate eNOS derived NO production , is a crucial determinant of NF kappaB activation and the lung inflammatory response to LPS . . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| However , MPs from T cells may induce endothelial dysfunction , altering gene expression of eNOS and caveolin 1 . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 , the caveolae scaffolding protein , binds to and negatively regulates eNOS activity . ^^^ As caveolin 1 also regulates caveolae mediated endocytosis after activation of the 60 kDa albumin binding glycoprotein gp 60 in endothelial cells , we addressed the possibility that endothelial NO synthase ( eNOS ) dependent NO production was functionally coupled to caveolae internalization . ^^^ Src activation induced the phosphorylation of caveolin 1 , Akt and eNOS , and promoted dissociation of eNOS from caveolin 1 . ^^^ In isolated perfused mouse lungs , gp 60 activation induced NO dependent vasodilation , whereas the response was attenuated in eNOS ( / ) or caveolin 1 ( / ) lungs . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| Caveolin 1 labeling was strongest in capillaries and did not coincide completely with ecNOS labeling in endocardial and venous endothelium . ^^^ |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P29474 and Q03135 |
Pubmed |
SVM Score :0.0 |
| NA |
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