| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| These results indicate that the Sp 1 site is essential for a core promoter activity of the human eNOS gene . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Gel shift and antibody supershift analysis using a BAEC nuclear extracts demonstrated four specific , DNA protein complexes binding to the eNOS Sp 1 site , with the slowest migrating form composed of Sp 1 and another nuclear protein . ^^^ These data demonstrate that the Sp 1 site is an important cis element in the core eNOS promoter . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| To elucidate the mechanism by which lyso PC increases the eNOS transcription , we identified Sp 1 sites at 104 to 90 and PEA 3 sites at 40 to 24 as being involved in lyso PC induced promoter activity . ^^^ These results suggest that lyso PC up regulates eNOS transcription by a PP2A dependent increase in Sp 1 binding activity . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Acute shear stress in vitro elicits rapid cytoskeletal remodeling and activates signaling cascades in ECs , with the consequent acute release of nitric oxide and prostacyclin ; activation of transcription factors nuclear factor ( NF ) kappaB , c fos , c jun and SP 1 ; and transcriptional activation of genes , including ICAM 1 , MCP 1 , tissue factor , platelet derived growth factor B ( PDGF B ) , transforming growth factor ( TGF ) beta 1 , cyclooxygenase 2 , and endothelial nitric oxide synthase ( eNOS ) . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Thus , cell specific eNOS expression in airway epithelium is dependent on the interaction of GATA 2 with the core eNOS promoter , and the proximal Sp 1 binding site is also an important positive regulatory element . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Our recent study indicates that lysophosphatidylcholine ( LPC ) enhances Sp 1 binding and Sp 1 dependent endothelial nitric oxide synthase ( eNOS ) promoter activity via the mitogen activated protein kinase / extracellular signal regulated kinase kinase 1 ( MEK 1 ) signaling pathway ( Cieslik , K . , Lee , C . ^^^ AG 490 , a selective inhibitor of Janus kinase 2 ( Jak 2 ) , also reduced the LPC induced Sp 1 binding and eNOS promoter activity to the basal level . ^^^ These results indicate that LPC enhances Sp 1 dependent eNOS promoter activity by a pertussis toxin sensitive , Ras independent novel pathway , PI 3Kgamma / Jak2 / MEK 1 / ERK1 / 2 . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Since hyperglycemia induced mitochondrial superoxide overproduction increases O linked N acetylglucosamine modification and decreases O linked phosphorylation of the transcription factor Sp 1 , the effect of hyperglycemia and the hexosamine pathway on eNOS was evaluated . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Its upregulation is mediated by an increased Sp 1 binding to its cognate site on eNOS promoter / enhancer region via the action of protein phosphatase 2A ( PP2A ) . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Estrogens can upregulate endothelial nitric oxide synthase ( eNOS ) in human endothelial cells by increasing eNOS promoter activity and enhancing the binding activity of the transcription factor Sp 1 . ^^^ In electrophoretic mobility shift assays , nuclear extracts from resveratrol incubated EA . hy 926 cells showed no enhanced binding activity of the eNOS promoter relevant transcription factors Sp 1 , GATA , PEA 3 , YY 1 , or Elf 1 . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| In fact , Sp 1 and MAZ bind to the same cis elements in the promoters of the genes for the receptor for serotonin 1A ( HT1Ar ) , endothelial nitric oxide synthase ( eNOS ) , phenylethanolamine N methyltransferase ( PNMT ) , the receptor for parathyroid hormone ( PTHr ) , MAZ and the major late promoter of adenovirus ( AdMLP ) . ^^^ Both Sp 1 and MAZ inhibited the expression of the gene for MAZ , while expression of the gene for eNOS was enhanced by Sp 1 and repressed by MAZ . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Like TNFalpha , Sp 1 binding to the eNOS promoter was decreased by NO * and a PKA inhibitor , H 89 , and increased by a PKA activator , dibutyryl cAMP ( Bt2cAMP ) . ^^^ Chromatin immunoprecipitation assays demonstrated that Sp 1 and to a lesser extent Sp 3 bound to the GC box regions of eNOS and TNFalpha in intact cells . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| The MEK 1 inhibitor , PD 98059 , also enhanced eNOS expression in native and cultured endothelial cells , an effect which was independent of ERK1 / 2 and associated with an increase in the DNA binding activity of AP 1 and Sp 1 . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| We addressed the functional consequences of methylation on eNOS transcription using transient transfection of in vitro methylated promoter / reporter constructs and found that methylated constructs exhibited a marked decrease in the synergistic action of Sp 1 , Sp 3 , and Ets 1 on eNOS promoter activity . ^^^ Importantly , chromatin immunoprecipitation demonstrated the presence of Sp 1 , Sp 3 , and Ets 1 at the native eNOS promoter in endothelial cells but not in vascular smooth muscle cells . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Cy3G enhanced the binding activity of the transcription factor Sp 1 to the GC box in the proximal eNOS promoter of BAECs , as revealed by chromatin immunoprecipitation assay . ^^^ The present study demonstrated that Cy3G induced eNOS expression and escalated NO production via an Src ERK1 / 2 Sp 1 signaling pathway in vascular endothelial cells . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| The proximal Sp 1 site is critical for basal activity of eNOS promoter . ^^^ The induction of eNOS by inhibition of HDACs in non endothelial cells , however , appeared not mediated by the changes in Sp 1 DNA binding activity . ^^^ We further showed that Sp 1 bound to the endogenous eNOS promoter and associated with HDAC 1 in non endothelial HeLa cells . ^^^ Combined TSA and AzadC treatment increased Sp 1 binding to the endogenous eNOS promoter but decreased the association between HDAC 1 and Sp 1 in HeLa cells . ^^^ Our data suggest that HDAC 1 plays a critical role in eNOS repression , and the proximal Sp 1 site may serve a key target for HDCA 1 mediated eNOS repression in non endothelial cells . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| These findings provide strong evidence that binding of Sp 1 or closely related protein to this site is required for the activation of basal NOS 3 transcription . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| SP 1 binding on NOS 3 promoter is critical for its constitutive expression . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| However , binding of transcription factor Sp 1 ( which is essential for the activity of the human NOS 3 promoter ) was significantly enhanced by estrogens . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Rather , several Sp 1 sites adjoining the putative NF kappaB binding site in the promoter region of NOS 3 gene are required for the induction of NOS 3 gene expression by Tat . . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| METHODS : The NOS 3 expression level and tumor microvessel density ( MVD ) status were determined via immunohistochemistry , using archived tissue specimens from 86 resected gastric cancer cases ; these findings were then correlated with Sp 1 expression and clinicopathological features . ^^^ In primary tumors , NOS 3 expression correlated highly with Sp 1 expression ( P = 0 . 001 ) and MVD status ( P = 0 . 001 ) . ^^^ Patients with strong Sp 1 expression were more likely to have strong NOS 3 expression ( 15 times ) and a high MVD ( 7 times ) than were those with negative Sp 1 expression . ^^^ In univariate survival analyses , strong NOS 3 expression ( P = 0 . 042 ) , strong Sp 1 expression ( P = 0 . 007 ) , and a high MVD ( P = 0 . 036 ) were associated with worse survival . ^^^ However , when patients ' NOS 3 and Sp 1 expression levels , MVD status , disease stage , completeness of resection , Lauren ' s classification , and age were entered in a Cox proportional hazards model , only strong NOS 3 ( P = 0 . 019 ) and Sp 1 expression ( P = 0 . 029 ) and advanced disease stage ( P = 0 . 006 ) were independently prognostic of poor survival . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Several features of the ecNOS are compatible with a constitutively expressed , poorly regulated gene , including absence of a TATA box and numerous SP 1 sites . ^^^ |
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| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
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