Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.63078701
Using SPR analysis we also found that native Ro 60 binds to La only in the presence of Ca2+ . 0.63078701^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The La protein , which is also complexed with the mutant 5S rRNA precursors , protects similar sequences from nuclease digestion as does the 60 kDa Ro protein . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The hY RNAs form a macromolecular complex with the 60 kDa Ro protein and may in addition be bound by the La protein . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
These included : Sm , RNP , DNA , RNA , RAP , SS A , and SS B . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
It has been established that there are several antinuclear antibodies that react with nuclear acidic proteins , and these include antibodies to Sm antigen , nuclear ribonucleoprotein , and SS A and SS B antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Characteristics of women participating in the family planning program of the Direccion General de Atencion Medica Materna Infantil y Planificacion Familiar de la SSA ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Three distinct precipitin systems were identified and referred to as precipitins SS A and SS B , and rheumatoid arthritis precipitin . ^^^ These studies show that precipitins SS A and SS B are present in high frequency in sera of patients with SS without associated rheuamtoid arthritis ( RA ) and are absent or present in low frequency in many other connective tissue diseases . ^^^ On the other hand patients with SS who also had clinical features compatible with RA ( SS RA ) did not have precipitins SS A and SS B but had rheumatoid arthritis precipitin ( RAP ) . ^^^ The origin of SS A and SS B antigens was demonstrated by immunofluorescence to be primarily from the nucleus but the origin of RAP in the cell has not been established . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The latter has been reported to be due to the presence of autoantibodies specific to La ( SS B ) and / or Ro ( SS A ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Since 1981 when anti Ro ( SS A ) and / or anti La ( SS B ) antibodies were described to be present in infants with neonatal lupus erythematosus ( NLE ) and their mothers , subsequent studies have demonstrated the almost universal association of NLE with either or both of these autoantibodies . ^^^ To our best knowledge , three cases of NLE were reported to be negative in anti Ro ( SS A ) and anti La ( SS B ) antibodies . ^^^ Both anti Ro ( SS A ) and anti La ( SS B ) antibodies were not detectable in his serum by immunodiffusion method while other such as RNP ( nonspecific , including U 1 , U 2 , U 3 , . . . , U 6 ) , Sm and Scl 70 antibodies were all positive . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Using immunoblotting technique ( IBT ) and saline extracts of rabbit thymus powder as antigen , we detected seven autoantibodies i . e . anti Sm , anti RNP , anti SSA , anti SSB , anti Scl 70 , anti Jo 1 and anti ribosome with only a strip of blot . ^^^ In rheumatic diseases , the antibodies against SSB could be detected more sensitively by IBT than by CIE ( P < 0 . 05 ) , while the antibodies against SSA could be detected more sensitively by CIE than by IBT ( P < 0 . 01 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Patients with antinuclear antibodies have no antibodies against native DNA , but in 5 cases , anti Ro ( SS A ) and anti La ( SS B ) antibodies were observed . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Four infants with neonatal lupus erythematosus who had annular erythema on the trunk and face reacted positively to tests for anti Ro ( SS A ) / La ( SS B ) antibodies . ^^^ Once anti Ro ( SS A ) / La ( SS B ) antibodies had turned negative , these tubuloreticular structures were no longer evident in the vascular endothelial cells in the same area where annular erythema had been present . ^^^ Mothers of these 4 infants reacted positively to anti Ro ( SS A ) / La ( SS B ) antibodies , but had no sicca syndrome . ^^^ These findings suggest that the presence of tubuloreticular structures may be related to anti Ro ( SS A ) / La ( SS B ) antibodies . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
These patients were tested for the antinuclear antibody , the C 3 and C 4 levels , anti ENA [ Ro ( SSA ) , La ( SSB ) , SM , RNP , scl 70 ] , anti single stranded DNA and anti double stranded DNA , lupus anticoagulant , anticardiolipin and antiphosphotidylserine . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Congenital complete AV block in SS A and SS B antibody positive collagenosis of the mother ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
METHODS : Serum from patients with characteristic cutaneous direct IF findings was examined for antinuclear antibodies by indirect IF and for antibodies to ribonucleoprotein , Sm , Ro ( SS A ) , and La ( SS B ) by gel immunodiffusion . ^^^ Ro ( SS A ) antibodies were identified in 23 of 26 patients and Ro ( SS A ) and / or La ( SS B ) in 25 of 26 patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Specific autoantibody identification , where possible , was usually of SS A or SS B although two sera from patients with Felty ' s syndrome had low levels of DNA antibody . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Laboratory investigations revealed : 24 hour urine protein excretion 3 g ; serum creatinine 5 . 6 mg / dl ; creatinine clearance 13 . 5 ml / min ; antinuclear factor strongly positive in a speckled pattern ; antibodies to nRNP positive with a titer of 1 : 20 , 480 ; antibodies to DNA , Sm , SS A , SS B , Scl 70 , centromere and Jo 1 negative ; serum complement normal . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Some of these monoclonal anti Ro ( SS A ) antibodies showed reactivity to DNA ( 2 / 19 ) , cardiolipin ( 9 / 19 ) , Sm / RNP ( 15 / 19 ) by ELISA , and to IgG ( 12 / 19 ) and La ( SS B ) ( 19 / 19 ) by ELISA and Western blotting . ^^^ Inhibition studies revealed that the binding to Ro ( SS A ) of both IgM hybridoma monoclonal and SLE serum polyclonal IgM anti Ro ( SS A ) antibodies was inhibited with Ro ( SS A ) , La ( SS B ) and Sm / RNP but not with IgG , DNA , RNA and BSA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : Sera from 31 patients with PPH and 24 with secondary pulmonary hypertension ( SPH ) were studied for the following autoantibodies : anti centromere ( indirect immunofluorescence of Hep 2 cells ) , anti CENP B by immunoblotting and enzyme immunoassay ( EIA ) using cloned CENP B fusion protein , anti topoisomerase 1 ( Scl 70 ) , anti Ku using immunoblotting of affinity purified antigens , anti cardiolipin using EIA , and anti Ro ( SS A ) , La ( SS B ) , Sm , nRNP , Jo 1 , PM Scl , and Mi 2 by counter current immunoelectrophoresis . ^^^ Antibodies to CENP B were found in one patient each with PPH and SPH , anti topoisomerase 1 in one with SPH , and anti Ro ( SS A ) and La ( SS B ) in one with PPH . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Conventional laboratory techniques , such as counter immunoelectrophoresis , have shown that antibodies to well defined `` extractable nuclear antigens ' ' ( eg , RNP , Sm , SS A , and SS B ) are not present in patients with juvenile chronic arthritis . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
SS patients frequently have a positive antinuclear antibody test due to presence of SS A ( Ro ) and SS B ( La ) autoantibodies . ^^^ Molecular analysis has revealed multiple `` SS A ' ' proteins ( 60 kd , 54 kd , 52 kd ) that react with sera from SS patients , as well as a 48 kd SS B protein . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Autoantibodies to SS A and to SS B were significantly associated with DR 3 ( P less than . 001 ) , HLA DQA 4 , ( P less than . 05 ) , and DQA4 / DQA1 heterozygotes ( P less than . 01 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Longitudinal studies of individual patients showed an association between disease exacerbation and enrichment of anti Ro ( SSA ) , anti La ( SSB ) and anti ssDNA . ^^^ Circulating immune complexes isolated by anti C 3 affinity chromatography were also found to contain anti Ro ( SSA ) , anti La ( SSB ) and anti ss DNA and fixation of complement by complexes could be demonstrated . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Using a 32P pulse chase labeled , adenovirus infected HeLa cellular extract as a source of antigen , anti La ( SS B ) and anti Ro ( SS A ) antibodies can be detected simultaneously using an immunoprecipitation assay . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Ro ( SS A ) and La ( SS B ) ribonucleoprotein complexes : structure , function and antigenicity . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
None had antibodies to Sm , RNP , Ro ( SS A ) , or La ( SS B ) by counterimmunoelectrophoresis . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We report the results of a panel of ANA determinations including ANA , anti dsDNA , Sm , RNP , SSA , SSB , Jo 1 , Scl 70 and PM 1 in 410 patients in a 5 year descriptive study of 410 patients with rheumatic disease symptoms of less than one year ' s duration . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Sjgren ' s syndrome associated with CREST and positive antibodies to Ro ( SSA ) / La ( SSB ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
HIV infection can occasionally result in an SS like disease but without the formation of antibodies to Ro ( SS A ) or La ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro ( SSA ) and anti La ( SSB ) antibodies in lupus erythematosus and Sjgren ' s syndrome . ^^^ Anti Ro ( SSA ) and La ( SSB ) antibody determinations have become important serologic tests in the evaluation of lupus erythematosus and Sjgren ' s syndrome patients . ^^^ This article will briefly review our knowledge of the molecular , immunogenetic and clinical features of the anti Ro ( SSA ) and anti La ( SSB ) antibody responses . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Associations between HLA class 2 antigens and autoantibodies to the ribonucleoproteins Ro ( SSA ) and La ( SSB ) have been reported in these patients . ^^^ This RFLP pair occurs in 76 % of patients with Ro ( SSA ) precipitins , 84 % of anti Ro ( SSA ) positive patients lacking La ( SSB ) precipitins , but only 41 % of the patients lacking both precipitins ( P = 0 . 0004 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Specific amino acid residues in the second hypervariable region of HLA DQA 1 and DQB 1 chain genes promote the Ro ( SS A ) / La ( SS B ) autoantibody responses . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In contrast to previous studies , a lower incidence of SS A or SS B ( 1 3 % ) , ANA ( 41 47 % ) , DNA ( 11 16 % ) , and RF ( 9 12 % ) serum antibodies was detected . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The pattern of CRI expression in the serum of 12 patients with rheumatoid arthritis ( RA ) , eight with systemic lupus erythematosus ( SLE ) and 20 with primary Sjgren ' s syndrome and 34 normal individuals were determined in parallel with the level of IgM RF , IgA RF and autoantibodies to the cellular antigens SS A , SS B , Sm , nRNP and dsDNA and cryoglobulins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To assess further the role of antinuclear antibodies in this process , peripheral blood lymphocytes ( PBMC ) were incubated with FITC conjugated IgG fractions from sera containing anti RNP ( anti RNP IgG ) , Ro ( SS A ) , La ( SS B ) and dsDNA antibodies and control sera for 24 h . ^^^ It was noted that anti RNP IgG entered 46 . 4 + / 7 . 2 % of lymphocytes which was significantly higher than anti Ro ( SS A ) ( 29 . 9 + / 4 . 1 % , P less than 0 . 05 ) , La ( SS B ) ( 22 . 0 + / 7 . 5 % , P less than 0 . 01 ) IgG and control IgG ( 28 . 8 + / 2 . 1 % , P less than 0 . 05 ) and not statistically different from anti dsDNA IgG ( 32 . 6 + / 14 . 3 % ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The protein extract prepared reacted in 2 with reference anti Ro ( SS A ) and anti La ( SS B ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In total 20 sera were tested for the presence of antibodies against nRNP , Sm , Ro ( SS A ) , La ( SS B ) , Scl 70 , centromeric antigens , ribosomal RNP and Jo 1 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
One hundred sixty four sera samples of patients with malignant diseases were analyzed for the presence of autoantibodies to ssDNA , dsDNA , poly ( 1 ) , Poly ( G ) , cardiolipin , histones , RNP , Sm , Ro ( SSA ) , and La ( SSB ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Patients with severe lymphocytic infiltration in minor salivary gland tissue rarely had kidney involvement and often had lymphadenopathy and circulating rheumatoid factor , cryoglobulins , and antibodies to SS A ( Ro ) and SS B ( La ) antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum antibody binding to histones , cardiolipin , ss A , ss B and Sm was found to be significantly higher in each of the MHS groups studied as compared to controls ( P less than 0 . 001 ) , and was also increased in the HIV+ patients vs . the HIV group ( P less than 0 . 05 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Clinical value of an integrated ELISA system for the detection of 6 autoantibodies ( ssDNA , dsDNA , Sm , RNP / Sm , SSA , and SSB ) . ^^^ An ELISA panel assay ( ANA / 6 ) which simultaneously detects IgG autoantibodies against 6 antigens ( ssDNA , dsDNA , Sm , RNP / Sm , SSA , and SSB ) was evaluated with sera from 98 patients with systemic lupus erythematosus ( SLE ) and related conditions , 68 disease controls , who were positive or negative by fluorescent ANA ( FANA ) , and 100 healthy controls . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
For the other types of nuclear fluorescence and for anti Sm , anti Ro ( SS A ) , anti La ( SS B ) , anti Scl 70 and anti Jo 1 , reference reagents are made available by the Arthritis Foundation ( AF ) and the Centers for Disease Control ( CDC ) in the USA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro ( SSA ) / La ( SSB ) antibodies and Sjgren ' s syndrome . ^^^ Antibodies to Ro ( SSA ) and La ( SSB ) cellular ribonucleoprotein complexes are found in the circulation of patients with Sjgren ' s syndrome ( SS ) , mainly in those with the primary form of the syndrome . ^^^ While anti Ro ( SSA ) antibodies are not specific for SS , anti La ( SSB ) antibodies seem to be specific . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The aim of the study was to investigate the prevalence of antinuclear and associated antibodies ( anti dsDNA , anticardiolipin , anti RNP , anti Sm , anti SSA and anti SSB ) and / or combinations thereof in systemic lupus erythematosus ( SLE ) patients with respect to their diagnostic and pathogenetic significance . ^^^ Regarding antibodies against RNP , Sm , SSA and SSB , the prevalence was found to be strongly influenced by the criteria used for patient selection . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
ACA positive sera were examined for antibodies against other nuclear antigens including nRNP , Sm , Scl 70 , SS A and SS B and cytoplasmic antigens by double immunodiffusion methods using rabbit thymus extract etc . as the antigens and by IF method using cryostat sections of rat kidney and stomach . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti nuclear antibodies were found in 93 p . 100 ; antibodies to Ro ( SSA ) and / or La ( SSB ) in 70 p . 100 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Decreased levels of autoantibodies against DNA , histones , cardiolipin , RNP , Sm , Ro ( SS A ) , and La ( SS B ) were detected in the sera of patients with uveitis receiving cyclosporine A or cyclosporine A plus bromocriptine following 3 months of treatment . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
A case control study of congenital heart block : association with maternal antibodies to Ro ( SS A ) and La ( SS B ) . ^^^ The odds of giving birth to a child with CHB were increased by a factor of 22 for women with antibodies to Ro ( SS A ) and / or La ( SS B ) compared with women who did not have these antibodies . ^^^ Thus the association between CHB and both anti Ro ( SS A ) and anti La ( SS B ) antibodies has been demonstrated in an Australian population and indicates that anti La ( SS B ) , as well as anti Ro ( SS A ) antibodies may be a risk factor for the development of CHB . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
After injection of monospecific sera directed against nDNA , Sm , nRNP , Ro ( SSA ) and La ( SSB ) , ANA were found in fetal circulation and trapped in the liver , spleen kidney and skin of fetus . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Isolated cases with IgG anti dsDNA , anti Ro ( SSA ) , and anti Ro ( SSA ) / anti La ( SSB ) were also identified . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The flare of SLE disease was characterized by raised levels of autoantibodies in the sera to dsDNA , histones , cardiolipin , Sm , RNP , SSA ( Ro ) , SSB ( La ) and an emergence of high titers of mouse antibody carrying the 16 / 6 Id . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
However , the coexistence of anti Ro ( SSA ) and anti La ( SSB ) antibodies ( 9 patients ) correlated significantly with HLA B 8 , whereas the haplotype HLA B8DR3 was more common in the anti Ro ( SSA ) positive patients than in the rest although the difference did not reach statistical significance . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Primary Sjgren ' s syndrome ( SS ) is considered a benign autoimmune disease ; it is characterized by lymphoid infiltration of salivary and lacrimal glands , often accompanied by the presence of serum autoantibodies , particularly anti Ro ( SS A ) and anti La ( SS B ) . ^^^ Only 1 of the 14 sera reacted against Ro ( SS A ) , and 1 other reacted against La ( SS B ) . ^^^ These results identify a subset of SS patients characterized by 1 ) the presence of serum antibodies to HIV 1 group specific , but not type specific , proteins , and 2 ) the relative absence of anti Ro ( SS A ) and anti La ( SS B ) autoantibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
No patient had antibodies against native deoxyribonucleic acid , ribonuclear protein , Ro ( SS A ) or La ( SS B ) antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The Ro ( SS A ) and La ( SS B ) antigenic determinants appear to be related to one another because of the frequent coincidence of spontaneous anti Ro and anti La in the same autoimmune sera , and because of a tendency of the Ro and La immunoprecipitin lines in double immunodiffusion analysis to fuse . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In whites , HLA DR 2 was associated with the presence of anti Ro ( SS A ) antibody while HLA DR 3 was associated with the presence of both anti Ro ( SS A ) and anti La ( SS B ) antibody . ^^^ In whites , the frequency of both anti Ro ( SS A ) and La ( SS B ) antibodies increased with increasing age as did that of HLA DR 3 ; HLA DR 2 , however , was more frequent in those with younger age at diagnosis . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In addition to anti La ( SS B ) reactivity , all 34 sera were shown to contain anti Ro ( SS A ) activity by counterimmunoelectrophoresis ( CIEP ) ; however , with three exceptions , the antigenic Ro ( SS A ) polypeptide was not detectable by immunoblotting . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The possibility that iRNP may be related to the small nuclear particles was tested by immunoreactivity with monospecific autoimmune antibodies to six antigenic determinants ( Sm , RNP , PM 1 , SS A ( Ro ) , SS B ( La ) , and Scl 70 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
At least part of the clinical heterogeneity is associated with serological heterogeneity and this is particularly striking among patients with SLE who produce anti Ro ( SSA ) and anti La ( SSB ) . ^^^ Moreover , among the patients with SLE who produce anti Ro ( SSA ) are 2 stable groups , on which produces anti Ro ( SSA ) alone and one which produces both anti Ro ( SSA ) and anti La ( SSB ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Autoantibodies to Ro ( SS A ) , La ( SS B ) , and Sm / nuclear RNP were quantitated by enzyme linked immunosorbent assay in 106 white patients with systemic lupus erythematosus . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Evidence is accumulating that antibodies to SSA ( and perhaps to SSB ) induce antibody dependent cell mediated cytotoxicity ( ADCC ) of basal keratinocytes in all of these lupus syndromes . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Intermediate blocking activity was conferred also by ribonucleic components as RNP , Ro ( SSA ) , and La ( SSB ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
This work is the first study of the ultrastructural muscle pathology in a patient with diagnoses of MCTD and SS based on clinical findings and the presence of antibodies to RNP , SS A and SS B antigens . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The two subtypes classified by Tran et al . , SSA and SSB , were confirmed and the studies on the relative amount of the subtypes revealed that more SSA subtype was expressed compared with SSB subtype during a week after birth , but , thereafter , both subtypes were almost equally expressed throughout the developmental stages tested . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to Ro ( SS A ) and La ( SS B ) were associated with DR 3 ( DRw 17 ) , DQw2 . 3 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The immunogenetic relationship between anti Ro ( SS A ) / La ( SS B ) antibody positive Sjgren ' s / lupus erythematosus overlap syndrome and the neonatal lupus syndrome . ^^^ Patients with Sjogren ' s / lupus erythematosus overlap syndrome , subacute cutaneous lupus erythematosus , and mothers of infants with the neonatal lupus syndrome characteristically have autoantibodies to Ro ( SS A ) , and in many cases , La ( SS B ) antigens . ^^^ The present study was designed to test the hypothesis that anti Ro ( SS A ) / La ( SS B ) positive Sjogren ' s / lupus overlap patients and mothers of infants with neonatal lupus erythematosus syndrome are immunogenetically homogenous and closely related . ^^^ These data demonstrate that anti Ro ( SS A ) / La ( SS B ) positive Sjogren ' s / lupus overlap patients and neonatal lupus syndrome mothers are immunogenetically closely related to each other and appear to be more closely related to both primary Sjogren ' s syndrome and subacute lupus erythematosus , than to classical systemic lupus erythematosus . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Of the 3 groups of sera studied , those containing anti Ro ( SS A ) alone had the highest degree of reactivity with human Ro ( SS A ) after absorption with bovine spleen extract , followed , in descending order , by sera containing anti Ro ( SS A ) and anti La ( SS B ) , and sera containing anti Ro ( SS A ) and anti nuclear RNP . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In an attempt to examine whether there exists a familial tendency in the production of such natural autoantibodies , 134 healthy members of 32 families were examined for antibodies against ss DNA , ds DNA , poly ( 1 ) , poly ( G ) , cardiolipin , histones , Sm , RNP , SS A ( Ro ) and SS B ( La ) , using an enzyme linked immunosorbent assay . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to Extractable Nuclear Antigens ( ENAs ) namely Sm , nRNP , SS A and SS B were studied in 397 patients with various connective tissue diseases ( CTD ) , 146 patients with inflammatory polyarthropathies , 16 cases of systemic vasculitides , and 39 normal subjects using counterimmunoelectrophoresis and double immunodiffusion methods . ^^^ The other associations included correlation of anti nRNP with pulmonary parenchymal lesions , anti SS A with serositis and pulmonary hypertension , and anti SS B with myocarditis and recurrent diarrhoea . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Both Ro ( SSA ) and La ( SSB ) antibody systems were positive in mother but only positive for La ( SSB ) antibody system in this baby . ^^^ We suggest that the Ro ( SSA ) and / or La ( SSB ) antibody systems may play a role in the pathogenesis of neonatal lupus erythematosus . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The neonatal lupus erythematosus syndrome , first described by McCuistion and Schoch in 1954 , is associated with characteristic skin lesions and congenital heart block in the new born , and the presence of Ro ( SSA ) , La ( SSB ) , or RNP antibodies in mothers and infants . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum antinuclear antibodies were present in one patient , but rheumatoid factor , SS A , and SS B antibodies were absent in all . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
No similar association was found for the presence or amount of antibody to Ro ( SS A ) in other first degree relatives or for antibody to La ( SS B ) or nuclear RNP in any relative . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The presence of the precipitating antinuclear antibodies SS A and / or SS B is pathognomonic for Sjgren ' s Syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Using the immunoblotting technique , sera from 433 patients with rheumatic diseases were screened for the presence of antibodies against several nuclear and cytoplasmic antigens , such as RNP , Sm , Ro ( SSA ) , La ( SSB ) , CR 19 ( centromeric antigen ) , Topo 1 ( Scl 70 ) , Jo 1 , histone and 56 kD . ^^^ However , some unexpected associations were found , for example polymyositis and calcinosis with anti Sm antibodies , sicca symptoms with anti centromere antibodies and leucopenia with Ro ( SSA ) and La ( SSB ) . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
For this purpose , we retrospectively analysed the results of tests for FANA ( on Hep 2 cells , positive FANA titers greater than or equal to 1 : 80 ) , anti ENA ( antibodies to Ro ( SSA ) , La ( SSB ) , U 1 nRNP and Sm , by counterimmunoelectrophoresis ) and anti dsDNA ( by enzyme immunoassay ) in the sera of 488 consecutive autoimmune patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Molecular characterization of Ro ( SSA ) and La ( SSB ) proteins . ^^^ Ro ( SSA ) and La ( SSB ) proteins were characterized using human spleen extract as antigen and monospecific anti Ro ( SSA ) and anti La ( SSB ) sera . ^^^ The anti Ro ( SSA ) sera had reacted with a polypeptidic band of 58 kilodaltons ( kDa ) , meanwhile the La ( SSB ) antisera reacted with 2 polypeptidic bands of 42 and 40 kDa . ^^^ The molecular weight of the Ro ( SSA ) and La ( SSB ) proteins obtained by gel filtration chromatography was in agreement with the PAGE / electrotransfer results . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Frequencies of autoantibodies to Sm , nRNP , Ro ( SSA ) and La ( SSB ) were determined by countercurrent immunoelectrophoresis ( CIE ) and / or enzyme linked immunosorbent assays ( ELISA ) in 106 whites and 60 blacks with systemic lupus erythematosus . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro ( SSA ) and anti La ( SSB ) antibodies in lupus erythematosus and in Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Elevated titers of autoantibodies reacting with DNA , poly ( 1 ) , poly ( dT ) , ribonucleoprotein , autoantigens [ Sm , SS A ( Ro ) , and SS B ( La ) ] , and cardiolipin were noted . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
However , in the group of 23 patients recently analyzed , 13 of 22 tested had anti Ro ( SS A ) and / or anti La ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
They commonly demonstrate rheumatoid factor , hypergammaglobulinemia , antinuclear and Ro ( SS A ) La ( SS B ) antibody activity . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Therefore an aggressive approach was taken to prevent CCHB in a fetus with a high risk for the condition as defined by ( 1 ) presence of DR 3 and high titers of antibodies to SSA ( Ro ) and SSB ( La ) in the mother ( 2 ) previous history of CCHB in a sibling . ^^^ The concentration of total maternal IgG and antibodies to SSA ( Ro ) and SSB ( La ) were decreased by greater than 60 % during the course of therapy . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Prevalence of maternal Ro ( SS A ) and La ( SS B ) autoantibodies in relation to congenital heart block . ^^^ All of 35 mothers of babies with congenital heart block , none of five mothers of babies with other types of heart block , 10 of 29 women with connective tissue disease but no babies with heart block , four of 445 normal pregnant women and two of 109 healthy nonpregnant women had either Ro ( SS A ) or La ( SS B ) antibodies . ^^^ The findings indicate that placental transfer of anti Ro ( SS A ) or anti La ( SS B ) is essential for development of congenital complete heart block . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to Ro ( SSA ) and La ( SSB ) antigens in Sjgren ' s disease and syndrome ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Clinical significance of antibodies to Ro ( SSA ) and La ( SSB ) ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
IgG ACA were not associated with other thromboembolic events , thrombocytopenia , serum IgG , or autoantibodies to Ro ( SSA ) , La ( SSB ) , U1RNP , Sm , or double or single stranded DNA . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
All these individuals were completely asymptomatic , and none of them had anti Ro ( SSA ) or anti La ( SSB ) autoantibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to nuclear antigens ( ANA ) , anti Ro ( SSA ) and anti La ( SSB ) as well as rheumatoid factor ( RF ) correlate with definite pSS , while in RA , ANA and anti Ro ( SSA ) are associated with the presence of sSS and anti La ( SSB ) is practically absent . ^^^ In pSS patients , the incidence of anti Ro ( SSA ) and anti La ( SSB ) , as well as the titers of ANA and RF , correlate with the degree of salivary lymphocytic infiltrates in class 1+ to 3+ . ^^^ In pSS patients , anti Ro ( SSA ) and anti La ( SSB ) correlate with earlier disease onset and longer disease duration , recurrent parotid gland enlargement ( RPGE ) , as well as with the presence of extraglandular manifestations ( extr . manif . ) particularly splenomegaly / lymphadenopathy and vasculitis . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro ( SS A ) and anti La ( SS B ) in patients with Sjgren ' s syndrome . ^^^ Clinical , serologic , and genetic findings in Sjgren ' s syndrome patients were correlated with quantitative determinations for antibody against Ro ( SS A ) , La ( SS B ) , and nRNP ( Sm ) using newly developed , sensitive solid phase assays . ^^^ In 86 Sjgren ' s syndrome patient sera , more than 96 % had anti Ro ( SS A ) , and 87 % had anti La ( SS B ) , spanning a 4 . 8 log 10 range of autoantibody concentration , whereas only 95 % of the patients had anti nRNP ( Sm ) . ^^^ Low levels of anti Ro ( SS A ) and anti La ( SS B ) were found in 10 % and 12 . 5 % , respectively , of the 40 normal control sera . ^^^ In Sjgren ' s syndrome patients , the level of anti Ro ( SS A ) correlated strongly with that of anti La ( SS B ) ( r = 0 . 80 ; P less than 0 . 0001 ) but not with the level of anti nRNP ( Sm ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
A sensitive and specific assay to detect Ro ( SS A ) and La ( SS B ) antibodies . ^^^ This paper describes a sensitive and specific immunoblotting procedure to detect Ro ( SS A ) and La ( SS B ) autoantibodies in the serum of patients with lupus erythematosus . ^^^ In order to perform this procedure , we have partially purified the Ro ( SS A ) and La ( SS B ) antigens from human spleen extracts by DEAE cellulose and Sephacryl S 300 chromatography . ^^^ The Ro ( SS A ) and La ( SS B ) antigens were immobilized on nitrocellulose paper after sodium dodecyl sulfate polyacrylamide gel electrophoresis and tested against a panel of sera at different dilutions : normal human sera ( n = 14 ) , Ro ( SS A ) antisera ( n = 6 ) , La ( SS B ) ( n = 7 ) , Ro ( SS A ) / La ( SS B ) ( n = 8 ) , Sm nRNP La ( SS B ) ( n = 2 ) . ^^^ We found that Ro ( SS A ) antisera react with a protein of an approximate Mr of 58K , whereas the La ( SS B ) antisera reacted with two bands of Mr 42 and 40K , respectively . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We studied the distribution in family members of antibodies to ss and dsDNA , and to Ro ( SSA ) , La ( SSB ) , Sm and nRNP . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Despite the evidence for the presence of the Ro ( SSA ) autoantigen both in the skin and heart and the almost universal presence of the Ro ( SSA ) autoantibody in mothers of infants with neonatal lupus , there are emerging data to suggest that La ( SSB ) and , rarely , nRNP antibodies play an important pathologic role in some cases of the neonatal lupus syndrome . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum samples from 64 apparently healthy individuals ( 32 men and 32 women , mean age 81 . 0 years ) were examined for the prevalence of several autoantibodies , including rheumatoid factor ( RF ) , antinuclear antibodies ( ANA ) , antibodies to extracted cellular antigens Ro ( SSA ) , La ( SSB ) , Sm , U1nRNP and Scl 70 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serological methods are described , such as examination of antibodies to nuclear antigens and their subunits SS A and SS B . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to soluble nuclear Scl 70 , Ul RNP and LA ( SSB ) antigens or to the cytoplasmatic Ro ( SSA ) antigen , respectively , were not detected in the patients with CS . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to Ro ( SS A ) and / or La ( SS B ) were present in only sixteen patients , and HLA DR 3 was found in only seventeen patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Finding low levels of anti Ro / SSA and anti La / SSB among normals may indicate that anti Ro / SSA and anti La / SSB occur in disease by enhancement of a preexisting immune response . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Significance and character of SS A ( Ro ) and SS B ( La ) antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The mother had a positive antinuclear antibody test result with antibodies directed against the Ro ( SSA ) and La ( SSB ) antigen system consistent with the diagnosis of Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
These data were correlated with serological markers ( antinuclear antibodies , single and double stranded anti DNA , anti SM , anti nRNP , anti Ro [ SS A ] , anti La [ SS B ] , and biological false positive tests for syphilis and clinical features . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
All three had Ro antibodies ( SS A ) in their sera , as well as La antibody ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The leukocytoclastic vasculopathy is associated with high titers of Ro ( SS A ) and La ( SS B ) autoantibodies ( detected by gel double diffusion techniques ) and general serohyperreactivity . ^^^ In marked contrast , the mononuclear vasculopathy is associated with low titers of Ro ( SS A ) and La ( SS B ) autoantibodies ( detected by enzyme linked immunosorbent assay but not gel double diffusion techniques ) and general serohyporeactivity . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antinuclear antibodies were also more prominent in the patients with airways disease , but antibodies against RNP , SS A , SS B , and double stranded DNA were not present in these patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In the sera of mothers , which have born children with a congenital total av block , antibodies of IgG class would be found , for instance anti Ro ( SS A ) and anti La ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
DR 3 correlated with DRw 52 ( p less than 0 . 0001 ) , anti Ro ( SSA ) ( p less than 0 . 0002 ) and anti La ( SSB ) ( p less than 0 . 02 ) antibodies , and extraglandular manifestations ( p less than 0 . 02 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Twenty two patients with aqueous tear deficiency ( ATD ) were examined for the presence of the following autoantibodies : immunofluorescent antinuclear antibody ( ANA ) and Sjgren ' s syndrome antibodies A and B ( SS A and SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In a study of the occurrence of detectable antibodies to SS A and SS B in 300 randomly selected mother infant pairs , three ( 1 % ) mother infant pairs were positive for precipitating antibodies to SS A . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The transplacental passage of SSA or SSB antibodies , of the IgG class , may mediate or be associated with immune damage to the fetal cardiac conduction system , as reported in our two patients . ^^^ Maternal and / or newborn screening for SSA and SSB antibodies in selected patients permits an early presumptive diagnosis and will assist perinatal planning , particularly for immediate newborn cardiac pacemaker implantation . ^^^ SSA or SSB positive maternal / fetal pairs should be prospectively managed by the obstetrician , neonatologist , and rheumatologist . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to the cellular ribonucleoproteins , Ro ( SSA ) and La ( SSB ) appear to have clinical significance in this condition , since not only do they identify patients at risk of developing Sjgren ' s syndrome but also they may have a pathogenetic role in this disease , at least in some of the systemic manifestations . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to nRNP , Sm , Ro ( SSA ) and La ( SSB ) detected by ELISA : their specificity and inter relations in connective tissue disease sera . ^^^ The profile of autoantibodies to four soluble cellular ribonucleoproteins nRNP , Sm Ro ( SSA ) and La ( SSB ) was determined using ELISA with immuno affinity purified antigens in a connective tissue disease population . ^^^ As previously demonstrated by immunodiffusion strong associations between anti nRNP and anti Sm and between anti Ro ( SSA ) and anti La ( SSB ) were detected by ELISA , while antibodies to nRNP and to Ro ( SSA ) identify distinctive serological groups . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Previously described precipitating antibodies ( anti Ro [ SSA ] and anti La [ SSB ] ) were restricted to a few patients with features of systemic lupus erythematosus ( SLE ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Although primary and secondary SS were most strongly associated with this DS specificity ( MT 2 ) , the anti Ro ( SS A ) and anti La ( SS B ) antibody responses were more closely allied to DR antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
By contrast , sera from FANA+ controls lacked anti Mit and anti SMus antibodies , but did contain antibodies to SSA , SSB , dsDNA , Sm , and RNP in much higher frequency than FACA+ sera . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In this study we used affinity chromatography with antibodies isolated from patients sera to purify two cellular antigens ; SS A ( Ro ) from human spleen and SS B ( La ) from rabbit thymus . ^^^ The protein components were acidic though SS A was resistant , and SS B sensitive to trypsin . ^^^ On polyacrylamide gel electrophoresis SS A migrated as a single 55K polypeptide and SS B as 40 45K polypeptides with a 29K degradation product . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Using CIE four distinct antibody antigen systems were detected : Ro ( SS A ) , La ( SS B ) and two new systems , designated XH and XR , reacting with extracts of human spleen and rabbit thymus , respectively . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
La ( SSB ) and / or Ro ( SSA ) antibody is almost universally present in the sera of the neonatal lupus mothers and their infants . ^^^ Since these antibodies may have a pathogenetic role in NLE , screening of infants with isolated CHB and / or cutaneous lesions suggestive of LE , and their mothers , for the presence of Ro ( SSA ) and La ( SSB ) antibodies is strongly recommended . ^^^ La ( SSB ) and / or Ro ( SSA ) antibody is almost universally present in the sera of the neonatal lupus mothers and their infants . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We measured the prevalence of ANA and antibodies against DNA , RNP , Sm , SSA , and SSB as well as antilymphocyte antibodies ( ALA ) in the serum of 24 patients with childhood onset SLE and 94 of their first degree relatives and compared the prevalence of these same antibodies in 8 patients with adult onset SLE and 33 of their first degree relatives . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to the defined nuclear antigens : deoxyribonucleic acid ( DNA ) , nuclear ribonucleoprotein ( nRNP ) , the Sm antigen , and Sjgren ' s syndrome A and B antigens ( SS A and SS B ) were examined to establish whether their presence was diagnostic of the associated diseases . ^^^ The frequency of antibodies to SS A and SS B did not differ from that in control subjects . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to Ro ( SSA ) and La ( SSB ) antigens were more common in patients with the extraglandular form of the syndrome but did not correlate with diffuse interstitial lung disease . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The clinical significance of autoantibodies to Ro ( SS A ) and La ( SS B ) in Sjgren ' s syndrome was examined in a retrospective evaluation of 75 patients with symptoms of the sicca complex who had either primary Sjgren ' s syndrome or Sjgren ' s syndrome associated with another connective tissue disease . ^^^ The clinical , hematologic , and serologic features associated with autoantibodies to the small molecular weight ribonucleoproteins Ro ( SS A ) and La ( SS B ) were ascertained . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The sicca complex is usually associated with Sjgren ' s syndrome and the presence of autoantibodies to SSA and SSB . ^^^ The clinician should consider more unusual causes of the sicca complex , eg , amyloidosis , particularly if the serologic markers of antibodies to SSA and SSB are absent . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
One hundred and twenty four sera from Greek patients with autoimmune rheumatic diseases ( 29 with systemic lupus erythematosus ( SLE ) , 24 with scleroderma , 11 with primary Sjgren ' s syndrome ( SS ) , and 60 with rheumatoid arthritis ( RA ) were tested for antibodies to nRNP , Sm , Scl 70 , Ro ( SSA ) , and La ( SSB ) cellular antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Strikingly , 12 had antibody to Ro ( SSA ) ( accompanied by anti La ( SSB ) in 8 ) compared to 36 % in the younger group . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Soluble SS A , SS B and RNP antinuclear antigen antibodies in 66 cases of pure or associated forms of Gougerot Sjgren syndrome ] . ^^^ In a prospective study , 66 patients fulfilling the criteria of Sjgren ' s syndrome were evaluated from the standpoint of the presence of soluble SS A , SS B and RNP specific nuclear antigen antibodies . ^^^ Anti SS A antibodies were found in 10 per cent of cases and anti SS B in 21 per cent , without there being any significant difference in prevalence between pure and associated forms of Sjgren ' s syndrome . ^^^ Three patients simultaneously showed anti SS A and anti SS B antibodies , four had only anti SS A and eleven anti SS B . ^^^ In this population of 66 patients with Sjgren ' s syndrome , no clinical manifestation appeared to be correlated with the presence of anti SS A and SS B antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to nuclear ribonucleoprotein ( nRNP ) , Sm , SS A , and SS B , were studied by a quantitative immunodiffusion technique in 48 patients with systemic lupus erythematosus ( SLE ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Of those with PSS and Sjgren ' s syndrome , 53 % had serum antibodies to SS A and / or SS B , while only 1 patient with a normal biopsy had either of these antibodies . ^^^ Anti SS A and anti SS B were not detected in patients with glandular fibrosis alone . ^^^ Antibodies to SS A and SS B are useful serologic markers of the presence of Sjgren ' s syndrome in patients with PSS . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The diagnosis of Sjgren ' s syndrome was established by demonstration of antibodies against SSA and SSB antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Thirty two patients with keratoconjunctivitis sicca ( KCS ) were screened for the presence of antinuclear antibodies , rheumatoid factor , and autoantibodies associated with Sjgren ' s syndrome ( designated SS A and SS B ) . ^^^ We found SS A and / or SS B autoantibodies in ten ( 31 % ) patients . ^^^ The presence of SS A and SS B autoantibodies correlated well with the clinical diagnosis of Sjgren ' s syndrome and seemed to identify the conditions of patients who may have a higher incidence of systemic complications with KCS . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies against soluble nuclear antigens of the SS A or SS B type . ^^^ Thirty five patients with systemic lupus were investigated for serum antibodies specifically directed against soluble proteic nuclear antigens similar to the SS A and SS B antigens . ^^^ Their prevalence in systemic lupus erythematosus was 35 % , anti SS A antibodies being more frequent than anti SS B antibodies ( 27 % and 4 , 5 % respectively ) . ^^^ These precipitating anti SS A or SS B antibodies have no prognostic significance . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Their sera were examined for antibodies to native deoxyribonucleic acid ( nDNA ) , nuclear ribonuclear protein ( nRNP ) , the non nucleic acid nuclear macromolecule Sm , the nuclear and cytoplasmic macromolecules La ( SSB ) and the predominantly cytoplasmic macromolecule Ro ( SSA ) . ^^^ These studies demonstrated that 42 % of the lupus patients possessed nDNA ; 36 % nRNP ; 24 % Ro ( SSA ) and 9 % anti La ( SSB ) antibodies . ^^^ Correlation to the cutaneous lesions among this lupus population possessing various serum antibodies revealed a trend toward photosensitivity and molar dermatitis in the Ro ( SSA ) , La ( SSB ) , Ro / la and the ANA negative , ANA negative / Ro positive groups of lupus patients . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serologic studies revealed elevated levels of serum IgG and IgM , rheumatoid factor , and antinuclear antibody with specificity for SS A and SS B antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Ro ( SSA ) and La ( SSB ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Ro ( SSA ) and La ( SSB ) antibodies in the clinical spectrum of Sjgren ' s syndrome . ^^^ Their status with respect to anti Ro ( SSA ) / La ( SSB ) antibodies was determined independent of clinical evaluation and categorization . ^^^ Overall , 33 ( 44 % ) were found to have antibodies to Ro ( SSA ) ; and 12 ( 16 % ) had antibodies to La ( SSB ) . ^^^ Furthermore , patients with SLE without the sicca complex demonstrated antibodies to Ro ( SSA ) ( 24 % ) and La ( SSB ) ( 5 % ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The infant ' s mother was asymptomatic but possessed anti Ro ( SSA ) and anti La ( SSB ) antibodies . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In order to find potential correlations between HLA class 2 alleles and anti SS A , SS B , Sm and anti snRNP responses among Norwegian patients with systemic lupus erythematosus ( SLE ) , HLA DRB 1 , DRB3 * 0101 , DQA 1 and DQB 1 alleles were determined by DNA typing 50 patients and 108 controls . ^^^ HLA distributions were analysed in the following autoantibody subgroups : anti SS A with SS B , anti SS A without SS B , anti snRNP without Sm , anti SS A without snRNP and anti snRNP without SS A . ^^^ Patients with anti SS A and SS B had significantly increased frequencies of DRB1 * 03 , DRB3 * 0101 , DQA1 * 0501 , DQB1 * 0201 ( in linkage disequilibrium ) versus controls and versus patients without anti SS A and SS B . ^^^ No differences in HLA distribution were found when the group with anti SS A alone was compared to the group with anti SS A and concomitant SS B . ^^^ Comparing the groups with and without anti SS A and SS B , the highest RR were found for the alleles DRB1 * 03 , DRB3 * 0101 , DQB1 * 0501 , DQB1 * 0201 ( in linkage disequilibrium ) with RR : 16 . 8 , 5 . 0 , 19 . 6 , 10 . 3 , respectively , P < 0 . 05 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Enhanced membrane expression of the 52 kDa Ro ( SS A ) and La ( SS B ) antigens by human keratinocytes induced by TNF alpha . ^^^ OBJECTIVE To investigate the membrane expression of the 52 kDa Ro ( SS A ) and La ( SS B ) antigens in human keratinocytes under the influence of an important mediator of inflammation , TNF alpha . ^^^ METHODS Keratinocytes , isolated from human skins obtained at circumcision and identified using monoclonal antibodies , were treated with tumour necrosis factor alpha ( TNF alpha ) and incubated with antibodies to 52 kDa Ro ( SS A ) isolated and purified from patients with systemic lupus erythematosus or Sjgren ' s syndrome , with mouse monoclonal antibody to La ( SS B ) , and ( as controls ) with sera from normal healthy blood donors and a mouse monoclonal antibody to U1RNP 68 kDa . ^^^ Membrane expression of the 52 kDa Ro ( SS A ) and La ( SS B ) antigens was detected using cyto enzyme linked immunosorbent assays ( ELISAs ) , laser scanning microscopy , and indirect immunofluorescence . ^^^ Indirect immunofluorescence with fixed normal human keratinocytes confirmed increased 52 kDa Ro ( SS A ) and La ( SS B ) antigen expression after the incubation with TNF alpha . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Rheumatoid factor was positive in 73 % of patients , antinuclear antibodies in 85 % , anti ( SS A ) in 62 % , and anti ( SS B ) in 46 % . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We therefore searched for hepatitis C virus RNA and antibodies in 48 patients with SS A / SS B autoantibodies . ^^^ METHODS : Patients with both SS A and SS B autoantibodies were included if stored serum samples and medical records were available . ^^^ CONCLUSIONS : We conclude that our patients with Sjgren ' s syndrome , without cryoglobulinemia and with detectable SS A / SS B autoantibodies , do not have clinically apparent liver disease , hepatitis C viremia by polymerase chain reaction , or antibodies to hepatitis C virus by second generation testing using RIBA 2 . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Human autoantibodies directed against the RNA recognition motif of La ( SS B ) bind to a conformational epitope present on the intact La ( SS B ) / Ro ( SS A ) ribonucleoprotein particle . ^^^ Autoantibodies affinity purified from the La RRM fragment to remove other specificities immunoprecipitated newly synthesized native La ( SS B ) / Ro ( SS A ) complexes , providing additional evidence that autoantibodies were recognizing a conformational epitope . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To study the association of maternal antibodies to Ro ( SSA ) and / or La ( SSB ) with isolated complete congenital heart block ( CCHB ) in children according to the child ' s age at detection . ^^^ CCHB detected before the age of 3 mo is highly associated with the presence of anti Ro ( SSA ) / La ( SSB ) in the mothers , while CCHB diagnosed later is generally not . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Since the prevalence of these types of proteins in patients with primary Sjgren ' s syndrome ( SS ) , systemic lupus erythematosus ( SLE ) , rheumatoid arthritis ( RA ) and autoimmune thyroid diseases has not been fully established , the aim of this work was to determine the prevalence of antibodies to immunodeficiency human virus type 1 ( HIV 1 ) proteins in these diseases and their possible relationship with the presence of anti nuclear , anti DNA , anti SSA ( Ro ) and anti SSB ( La ) autoantibodies . ^^^ Antinuclear antibodies , anti DNA , anti SSA ( Ro ) and anti SSB ( La ) were determined by enzyme linked immunosorbent assays . ^^^ These antibodies were not associated with autoantibodies ANA , DNA , SSA ( Ro ) or SSB ( La ) . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Neonatal lupus syndrome ( NLS ) is characterized by the presence of cutaneous lupus , congenital heart block or both associated with maternal autoantibodies anti Ro ( SS A ) and anti La ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Two of the three EIAs that detected immunoglobulin G type autoantibodies to Smith ( Sm ) , ribonucleoprotein ( RNP ) , Sjgren ' s syndrome associated antigens Ro ( SSA ) and La ( SSB ) , were provided by INOVA and Sigma Diagnostics . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
All 250 sera were tested blindly using a panel of 20 autoantigens including SS A , SS B , RNP , cardiolipin ( CL ) , collagen types 1 , 2 and 4 , phosphatidylserine ( PS ) , myeloperoxidase ( MPO ) , sulfatides ( sulf ) , thyroglobulin ( TG ) , gangliosides ( GDIa ; GM 2 ) , proteinase 3 ( PR 3 ) , Jo 1 , Sm , HPRPP ribosomal phosphate , histones ( H2AH2B ) , Scl 70 and glomerular basement membrane ( NC 1 ) . ^^^ There was a statistically significant greater frequency of autoantibodies in women with implants for 15 of the 20 autoantigens ; these were particularly striking for anti H2AH2B , HPRPP , SS A , SS B , Scl 70 , CL , PS , GM 2 , and NC 1 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
A previously affected child , high titers of anti Ro antibodies , the presence of anti Ro ( SS A ) and anti La ( SS B ) , and maternal HLA DR 3 confer high risk . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The prevalence of positive antibodies to nucleus , smooth muscle , and single stranded DNA in the patients ( 26 % , 36 % , and 34 % , respectively ) was higher than that in the control group ( 8 % , 4 % , and 4 % , respectively ) , although neither group was positive for autoantibodies against double stranded DNA , extractable nuclear antigen , SS A , SS B , mitochondria , or rheumatoid factor . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We used saline extracts of human spleen cells and rabbit thymus extract to detect six subtypes of anti ENA antibodies , namely anti SSA ( anti Sjogren ' s syndrome A ) , anti SSB ( anti Sjogren ' s syndrome B ) , anti RNP ( anti Ribonucleoprotein ) , anti Sm ( anti Smith ) , anti Jo 1 ( anti Jo 1 ) and anti Scl 70 ( anti Systemic sclerosis 70 ) antibodies . ^^^ Fifty seven were positive for auto antibodies , of which 26 had anti SSA antibodies , 12 anti SSA+ anti RNP+ anti Sm antibodies , 7 anti RNP+ anti Sm antibodies , 6 anti SSA+ anti SSB antibodies , 5 anti SSA+ anti RNP antibodies while one patient showed only anti RNP antibody . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
SS A and SS B was not detected . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Autoimmunity to La ( SS B ) and Ro ( SS A ) is a paradigm for understanding the normal mechanisms of B cell and T cell tolerance and development of autoimmunity to clinically important sequestered autoantigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Counterimmunoelectrophoresis for antibodies to Sm , RNP , SS A and SS B were negative . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Correlations were made between seroreactivity to B . burgdorferi and standard serological tests of autoimmunity : antibodies to nuclear antigens , dsDNA , cardiolipin , SSA and SSB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Despite clinical features suggesting Sjgren ' s syndrome , antibodies to Ro ( SSA ) were seen in only 2 patients , and antibodies to La ( SSB ) were seen in only 4 patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The test system was evaluated with 158 sera that were positive by immunofluorescence ( IF ) , some of which were immunologically characterized as containing antibodies to Sm , RNP , SS A , SS B , nucleolar , and DNA , and 247 IF negative that were used as a control group . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro ( SSA ) and anti La ( SSB ) antibodies in autoimmune rheumatic diseases . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Partial sequences of both inserts were identical , but differed from DNA encoding the Ro ( SSA ) and La ( SSB ) antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The latter has been reported to be due to the presence of autoantibodies specific to La ( SS B ) and / or Ro ( SS A ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antinuclear antibodies , especially anti Ro ( SSA ) and anti La ( SSB ) were also more common in primary SS ( p < 0 . 05 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Relationship between maternal and neonatal levels of antibodies to 48 kDa SSB ( La ) , 52 kDa SSA ( Ro ) , and 60 kDa SSA ( Ro ) in pregnancies complicated by congenital heart block . ^^^ Since there is suggestive but , to date , indirect evidence that maternal anti SSA ( Ro ) and SSB ( La ) antibodies are pathogenic in congenital heart block ( CHB ) , we explored the hypothesis that binding to fetal tissues would result in selective depletion of autoantibodies from the neonatal circulation . ^^^ Maternal and umbilical cord levels of anti 48 kDa SSB ( La ) , anti 52 kDa SSA ( Ro ) and anti 60 kDa SSA ( Ro ) antibodies were measured by ELISA and immunoprecipitation at parturition in 15 pregnancies complicated by CHB . ^^^ A control group consisted of 13 pregnancies in which the mother was known to have antibodies to either SSA ( Ro ) and / or SSB ( La ) and the children did not have CHB . ^^^ The ratios of maternal to cord serum levels of anti 48 SSB ( La ) , anti 52 SSA ( Ro ) and anti 60 kDa SSA ( Ro ) antibodies ranged from 0 . 71 to 2 . 38 in both affected and unaffected pregnancies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Intra and intermolecular spreading of autoimmunity involving the nuclear self antigens La ( SS B ) and Ro ( SS A ) . ^^^ We have tested the extent of immune self tolerance to the ubiquitously expressed nuclear / cytoplasmic autoantigens La ( SS B ) and Ro ( SS A ) in healthy , nonautoimmune mice . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Ydj1p cooperated with Ssa Hsp 70 proteins in the prevention of protein aggregation , but not with the Ssb Hsp 70 proteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti nuclear antibodies showed the highest reactivity against native DNA ( 70 % of patients ) , which was followed by binding to SS A , eRNP and SS B antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
For clinical purposes the most important antinuclear antibodies are antibodies against double stranded DNA , Sm , RNP , SS A , SS B , centromere proteins and Jo 1 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NLE is a rare condition affecting newborn infants of mothers who have connective tissue disease , with or without autoantibodies to extractable nuclear antigens Ro ( SS A ) , La ( SS B ) or ribonucleoproteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Of particular interest have been antibodies to proteins with nucleic acid binding potential , such as Sm , SS A ( Ro ) , and SS B ( La ) . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To probe the role of cytoplasmic heat shock protein 70 ( Hsp 70 ) chaperones in Trp 3 protein folding , anthranilate synthase and indoleglycerol phosphate synthase activities were measured in ssa and ssb mutants . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
A high level of antinuclear antibodies to SSA and SSB was associated with inflammatory lesions in minor salivary glands biopsy samples consistent with the diagnosis of Sjgren ' s syndrome . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Kits A and 5 were sensitive especially for the detection of SSA ( Ro ) and SSB ( La ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Purified antibodies to Ro ( SS A ) , La ( SS B ) and RNP served as controls . ^^^ Antibodies to Ro ( SS A ) , La ( SS B ) or RNP had no cytotoxic effect . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Endothelial cell derived factors in relation to antibodies to SSA or SSB and to endothelial cells in Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In Saccharomyces cerevisiae Ydj1p , a DnaJ homolog , is localized to the cytosol with the Ssa and Ssb Hsp 70 proteins . ^^^ In this study , the role of Ydj1p in modulating ATP dependent reactions catalyzed by Ssa and Ssb Hsp 70 proteins has been examined using purified components and compared with that of other Hsp 70 homologs BiP and DnaK . ^^^ Therefore the chaperone related activities of Ssa and Ssb Hsp 70 proteins exhibit significant differences in sensitivity to ATP and YDJ1p . ^^^ The specificity of interactions between Ydj1p and the Ssa and Ssb Hsp 70 proteins observed could contribute in determining the functional specificity of these chaperones in the cytosol . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum from the mother and cord blood from the infants revealed Ro ( SSA ) and La ( SSB ) antibodies in all three sera . ^^^ This study indicates that placental transfer of anti Ro ( SSA ) or anti La ( SSB ) alone to the fetus is not sufficient for the expression of congenital complete heart block . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Here we present the nucleotide sequence of the SSA 3 and SSB 2 genes , completing the nucleotide sequence data for the yeast HSP 70 family . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The prevalences of leucopenia and antibodies to extractable nuclear antigens in particular anti Ro ( SSA ) and anti La ( SSB ) , were lower in men . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Using an ELISA with purified ( SSA ) Ro and ( SSB ) La proteins derived from a human cell line as antigens , 34 % of the women were anti SSB positive and 46 % were anti SSA positive at followup . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Detection of autoantibodies to Ro ( SS A ) , La ( SS B ) and U1RNP in different congenital heart rhythm disorders using immunoblot and enzyme immunoassay . ^^^ Sera of fifty three infants with different heart rhythm disorders and eight unaffected children born to SLE mothers were tested for antibodies to Ro ( SS A ) , La ( SS B ) and U1RNP ( including recombinant A , C , 68 kD proteins ) using enzyme immunoassays and immunoblot . 21 sera from affected and 8 sera from healthy children reacted in counter immunoelectrophoresis with Ro ( SS A ) . 8 / 8 infants with third degree atrioventricular ( AV ) block , 4 / 8 with first degree AV block , one with sinusbradyarrhythmia ( SB ) and another with SB / first degree AV block were anti 52 kD Ro ( SS A ) positive compared to 7 / 8 healthy infants . ^^^ It was concluded that the influence of antibody levels to the 52 kD Ro ( SS A ) and La ( SS B ) components and the association of anti 52 kD Ro ( SS A ) , La ( SS B ) and 60 kD Ro ( SS A ) antibodies , as well as the sex , on the severity of congenital AV conduction defects suggests involvement of these factors in the pathogenesis of these disorders . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
It is invariably accompanied by the presence of SS A and SS B autoantibodies in the mother . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibody recognition of the recombinant human nuclear antigens RNP 70 kD , SS A , SS B , Sm B , and Sm D by autoimmune sera . ^^^ Five human nuclear antigens , RNP 70 kD , SS A , SS B , Sm B and Sm D , were produced in E . coli using the expression vector pSEM . cDNAs encoding these antigens were ligated to a truncated lacZ ' gene of the vector and the beta galactosidase fusion proteins were efficiently expressed as intracellular inclusion bodies after isopropyl beta thiogalactopyranoside induction . ^^^ The three fusion proteins , RNP 70 kD , SS B , and Sm B , showed good reactivities in both systems , whereas the other two fusion proteins , SS A and Sm D , showed poor and no reactivity in both systems , respectively . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Both mother and infant were positive for Sjgren syndrome A+B antibodies ( SS A [ Ro ] and SS B [ La ] ) as well as having high titres of antinuclear antibody ( ANA ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In addition , anti Ro ( SSA ) antibodies were associated with anti La ( SSB ) and RF , while anti Sm antibodies were associated with anti nRNP and RF . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Congenital auriculo ventricular block and anti Ro ( SS A ) / anti La ( SS B ) antibody ] . ^^^ The authors discussing the case find the most likely link : an anti RO ( SS A ) and anti LA ( SS B ) immunological block and they suggest that there are minor localised lesions in the nodal tissue which gives rise to benign disturbances of cardiac rhythm and they point out ways of preventing intrauterine auriculo ventricular block . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
No direct correlation between HLA DPB 1 and antibodies against recombinant Ro ( SS A ) / La ( SS B ) proteins in systemic lupus erythematosus . ^^^ We investigated the association of HLA DPB 1 alleles with the occurrence of autoantibodies against Ro ( SS A ) or La ( SS B ) using recombinant 52 kD Ro , 60 kD Ro and La proteins in 177 German patients with systemic lupus erythematosus ( SLE ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The patient had elevated Ro ( SSA ) and La ( SSB ) antibody titers , and studies performed on maternal blood disclosed positive antinuclear antibodies and elevated Ro and La antibody titers . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The patient had an increased erythrocyte sedimentation rate , pancytopenia , and positive tests for antibodies to nuclear antigen , SS A , and SS B . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To discover genetic and immunologic events governing expression of a new SLE associated antibody , the presence of anti Su and its relationship to other SLE related antibodies ( anti Sm , ribonucleoprotein , Ro ( SS A ) , La ( SS B ) ) were determined in MRL and other autoimmune and nonautoimmune mice . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum levels of von Willebrand factor antigen ( as an index of damage to the endothelium ) and tissue antinuclear antibodies and antibodies to SSA ( Ro ) ( which have been associated with vasculitis ) and SSB ( La ) autoantigens were measured in patients with Sjgren ' s syndrome . ^^^ Damage to the endothelium in Sjgren ' s syndrome : lack of correlation with antinuclear antibody titre and presence of antibodies to SSA or SSB autoantigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
RESULTS The patient had xerostomia , an abnormal result of a salivary gland biopsy , SSA and SSB antibodies , and the histocompatibility antigens HLA B 8 and HLA DR 3 that are associated with Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Presence of anti La ( SS B ) is associated with binding to the 13 kD carboxyl terminus of 60 kD Ro ( SS A ) in systemic lupus erythematosus . ^^^ All nine sera with Ro ( SS A ) and La ( SS B ) Ouchterlony immunodiffusion precipitins bound 13 kD ( p = 0 . 01 ) , whereas 10 of 11 sera with both anti Ro ( SS A ) and anti La ( SS B ) as determined by immunosorbent assay bound 13 kD ( p = 0 . 002 ) . ^^^ Inhibition studies demonstrated that antibodies binding the 13 kD fragment bound the 60 kD Ro ( SS A ) protein but did not bind the La ( SS B ) protein . ^^^ Thus , anti La ( SS B ) was found in those sera that bound epitopes within the 13 kD carboxyl terminal of 60 kD Ro ( SS A ) . ^^^ These data suggest a structural basis by which anti Ro ( SS A ) and anti La ( SS B ) are coupled in SLE . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The anti 52 kDa antibodies detected by IB HeLa correlated to those found by IB RBC ( P < 0 . 001 ) and occurred predominantly in primary Sjgren ' s syndrome ( P < 0 . 001 , sensitivity : 71 and 77 % ) as well as in sera with anti Ro ( SSA ) and anti La ( SSB ) antibodies ( P < 0 . 001 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
After preincubation with purified anti PR 3 antibody , purified control antibodies ( SS A , SS B , RNP ) ( IgG and F ( ab ' ) 2 fragments ) or different cytokines ( controls ) , VCAM 1 was detected on the surface of unfixed HEC by cyto ELISA and polymerase chain reaction analysis . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to Ro ( SS A ) and La ( SS B ) are found in about 50 % of patients and are associated with more severe glandular and extraglandular manifestations . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Significantly increased maternal and fetal IgG autoantibody levels to 52 kD Ro ( SS A ) and La ( SS B ) in complete congenital heart block . ^^^ Anti 60 , 52 kD Ro ( SS A ) and La ( SS B ) IgG antibodies coincided in 9 / 16 CHB cases , exclusively ( P < 0 . 05 ) . ^^^ Associated anti 52 kD Ro ( SS A ) and La ( SS B ) IgG antibodies were detected in 14 / 16 CHB and in 3 / 8 control cases ( P < 0 . 05 ) . ^^^ The fetal CHB sera contained significantly higher IgG levels of anti 52 kD Ro ( SS A ) ( P < 0 . 005 ) and La ( S B ) ( P < 0 . 015 ) compared to control samples . ^^^ Sera from mothers with CHB children had significantly higher levels of anti 52 kD Ro ( SS A ) ( P < 0 . 015 ) and La ( SS B ) ( P < 0 . 015 ) IgG antibodies than those of the control group . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to nRNP ( U 1 RNP / snRNP ) ; Sm ; SS A ; SS B ; Scl 70 ; thyroid microsome ; immunoglobulin ( Ig ) G , IgM , and IgA antibodies to cardiolipin ; and antibodies to native and denatured human types 1 and 2 collagen were measured by enzyme linked immunosorbent assay . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Hypergammaglobulinemic purpura in systemic autoimmune rheumatic diseases : predictive value of anti Ro ( SSA ) and anti La ( SSB ) antibodies and treatment with indomethacin and hydroxychloroquine . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Bulk cultured T cells and T cell lines were examined for the proliferative response to recombinant Ro ( SSA ) 52 kDa and SSB proteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Samples with discordant results between IFA and EIA were further analyzed using single antigen EIAs for SSA , SSB , Sm , RNP , Scl 70 , histones , dsDNA , and ssDNA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Systemic lupus erythematosus is characterized by the production of Abs to ribonucleoproteins , including Ro ( or SS A ) and La ( or SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In her second pregnancy plasma hyperviscosity and high antibody reactivities to 52 / 60 kD Ro ( SS A ) and La ( SS B ) represented elevated risk factors for abortion and fetal congenital heart block . ^^^ Interestingly , antibody levels to the anti Ro ( SS A ) / La ( SS B ) complex showed an increase during both pregnancies with a decline after the abortion as well as the partus . ^^^ The combined therapy led to a decrease in both the autoantibody reactivities to 52 kD Ro ( SS A ) and La ( SS B ) and the plasmaviscosity . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
At least four of the six items in the criteria set ( limiting item 6 to the presence of Ro ( SS A ) or La ( SS B ) antibodies ) were present in 79 of 81 patients initially classified as having primary SS ( sensitivity 97 . 5 % ) , but in only seven of 121 non SS controls ( specificity 94 . 2 % ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Subclass distribution of maternal and neonatal anti Ro ( SSA ) and La ( SSB ) antibodies in congenital heart block . ^^^ To compare the subclass distribution of anti 48 kDa La ( SSB ) and anti 52 and 60 kDa Ro ( SSA ) antibodies in the maternal and neonatal circulation , in pregnancies affected and unaffected by the development of congenital heart block ( CHB ) . ^^^ Sera were obtained from 32 mothers ( during 34 pregnancies 23 complicated by CHB and 11 healthy ) demonstrated to have anti Ro ( SSA ) and / or La ( SSB ) . ^^^ All 4 subclasses of anti Ro ( SSA ) and La ( SSB ) antibodies cross the placenta and are detectable in sera obtained from the umbilical cord , IgG 1 and IgG 3 were the major subclasses represented in the 48 kDa La ( SSB ) and 52 kDa Ro ( SSA ) responses . ^^^ All subclasses , including IgG 2 and IgG 4 , were observed in about one third of the anti 52 kDa Ro ( SSA ) and 48 kDa La ( SSB ) responses . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Homology ranging from 65 to 76 % was observed with members of four subfamilies ( SSA , SSB , SSC , and SSD ) of the Saccharomyces cerevisiae HSP 70 gene family . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Furthermore , the method can identify sera likely to contain autoantibodies commonly detected in ANA positive sera ( SS A , SS B , Sm , small nuclear ribonucleoprotein , Scl 70 , and double stranded DNA ) . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The other regions studied contained various proportions of SSA and SSB subtypes . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Sera from anti Ro ( SSA ) positive and negative patients with rheumatoid arthritis ( RA ) were compared with those from patients with primary Sjogren ' s syndrome ( pSS ) and systemic lupus erythematosus ( SLE ) with regard to anti La ( SSB ) antibodies . ^^^ Several assays for anti La ( SSB ) ( RNA precipitation , counterimmunoelectrophoresis , and immunoblotting ) demonstrated the presence of such antibodies in selected anti Ro ( SSA ) positive ( 10 / 19 in RA and 18 / 37 in pSS and SLE ) but not in anti Ro ( SSA ) negative sera . ^^^ These results were further supported by the analysis of a cohort of 70 consecutively selected sera ( displaying monospecific anti Ro ( SSA ) reactivity against calf thymus extract ) from patients with various rheumatic disorders , where human specific anti La ( SSB ) were detected in two out of the five of RA patients studied . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Her mother had no cutaneous or other lesions , but complement examinations revealed the presence of anti Ro ( SSA ) and anti La ( SSB ) antibodies , and absence of anti Sm and anti RNP antibodies . ^^^ Ro ( SSA ) and La ( SSB ) antibodies were found , and direct immunofluorescence testing on healthy skin was positive . ^^^ At age 5 months , follow up immunologic examination of the infant had normal results but the mother still had anti Ro ( SSA ) and anti La ( SSB ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Sera from 23 patients with paraneoplastic disease of the central nervous system ( PNS ) were examined for the presence of anti neuronal ( anti Hu , anti Yo / PCA ) and anti Ri ) and systemic auto antibodies , including antibodies against DNA , centromeres , nRNP , Sm antigen , Scl 70 , Ro ( SS A ) , La ( SS B ) , mitochondria , thyroid antigens , parietal calls , brush border antigen and rheumatoid factor . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Patients with Sjgren ' s syndrome frequently have autoantibodies to La , 60 kDa Ro ( SS A ) protein ( Ro 60 ) , and 52 kDa Ro ( SS A ) protein ( Ro 52 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Immunological tests : serum titers of anti nuclear ( ANA ) , anti DNA , anti ENA ( SS A , SS B , RNP , FR ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
It was found that the P P G M R P P epitope is recognized mainly by anti U1RNP and / or anti Sm positive sera , but also by anti Ro ( SSA ) ( hY1RNA protein complex ) and anti La ( SSB ) ( hY1RNA protein complex ) positive sera , although these sera are negative for anti U1RNP and anti Sm . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To address these issues , we have examined the mouse immune response to La autoantigen ( mLa ) and the homologous human La antigen ( hLa ) , which are components of the La ( SS B ) / Ro ( SS A ) ribonucleoprotein ( RNP ) complex targeted in systemic lupus erythematosus and primary Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
For the detection of autoantibodies we used the Elisa technique using the following purified antigens in an Elisa assay : dsDNA , ssDNA , histone , mitochondria , RNA , RNP , SS A , SS B , Sm , Scl 70 , Anca C , Anca P and the cardiolipin complex . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti La ( SS B ) antibodies were significantly associated with anti 52 kd Ro ( SS A ) in sera from patients with pSS compared with PBC patients ( P < . 025 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Congenital complete heart block is more frequently seen in infants of mothers with systemic lupus erythematosus , both clinically manifested and subclinical systemic lupus erythematosus with positive antibodies ( SS A and SS B antibodies ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The disease is mostly associated with transplacental passage of maternal anti Ro ( SS A ) or anti La ( SS B ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro ( SS A ) and anti La ( SS B ) antibodies were detected in nine ( 60 % ) and seven ( 47 % ) patients , respectively . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We concluded that SSA is safe and excellent approach for mitral valve surgery , especially in patients who require reoperations and in patients with small LA , although we should consider the advantages of SSA versus the risks of post operative arrhythmia and bradycardia . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We found a correlation between the sIL 2 R level and the presence of SS A and SS B antibodies or antinuclear antibody levels , and no correlation with other serological and clinical markers . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Social Support Appraisals ( SS A ) and Social Support Behaviours ( SS B ) Scales . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Both were male with lung and stomach non Hodgkin ' s lymphoma respectively , enlargement of the lacrimal glands , monoclonal gammapathy of the IgM kappa type and , one of them had high titer and of fine speckled pattern positive antinuclear antibodies and anti Ro ( SSA ) and anti La ( SSB ) antibodies in his serum . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to the nuclear antigens SSA and SSB were found in respectively 22 . 2 % and 15 . 6 % of the patients . ^^^ Two out of ten patients with both anti SSA and anti SSB antibodies gave birth to a child with heart block . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro ( SSA ) and anti La ( SSB ) antibodies and complete congenital heart block . ^^^ Anti Ro ( SSA ) and anti La ( SSB ) antibodies are usually detected in sera of mothers of children with prenatally diagnosed CCHB and in cardiac tissues of affected newborns . ^^^ Assays for anti Ro ( SSA ) and anti La ( SSB ) antibodies should be performed on sera of pregnant women with SLE and newborns with CCHB . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Clinical findings used include plasma SC5b 9 level , SSA , SSB , Sm , RNP , ANA antibodies , and SLE complications ( SLE nephritis , pneumonia & encephalopathy ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NLE is a rare condition which affects newborn infants from mothers who may me asymptomatic or have a connective tissue disease , with or without autoantibodies to extractable nuclear antigens Ro ( SS A ) , LA ( SS B ) or ribonucleoproteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Autoantibodies reactive with Ro ( SSA ) and La ( SSB ) and pregnancy . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum samples from 350 patients with connective tissue diseases ( CTD ) , and from 300 normal subjects were assayed for ANA by ELISA and the results were compared with those of FANA and of single antigen ELISAs for RNP , Sm , SS A , SS B , Scl 70 , Jo 1 , dsDNA , and ssDNA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Specimens were tested by three different EIA assays for autoantibodies to SS A , SS B , Scl 70 Sm , RNP , Jo 1 , ENA , Histone , ss DNA and ds DNA and one IF assay for Antinuclear Antibodies ( ANA ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In conclusion , CIE concurs mostly with ELISA for anti RNP , Scl 70 , Sm and SS B antibodies , but with some disagreement for SS A antibodies . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In HCV associated sicca syndrom , antibodies against Ro ( SSA ) and La ( SSB ) are not detected . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The association between this disease , maternal connective tissue disease and maternal antibodies [ anti Ro ( SS A ) resp . anti La ( SS B ) ] is well known . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies ( anti Ro [ SS A ] , anti La [ SS B ] , rheumatoid factor , antinuclear antibody ) were measured . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Finkelstein et al . : `` Anti Ro ( SSA ) and anti La ( SSB ) antibodies and complete congenital heart block ' ' ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Finkelstein et al . : `` Anti Ro ( SSA ) anti La ( SSB ) antibodies and complete congenital heart block ' ' . ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Her serologic tests revealed positive autoantibodies ( ANA , SSA ; SSB & RF ) , and a lower lip biopsy confirmed the diagnosis of Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies to Sm and U1RNP were absent ; anti Ro ( SSA ) and anti La ( SSB ) occurred in 6 % , while anti dsDNA occurred in less than 10 % of the cases . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The HLA DRB1 * 15 * 03 heterozygote genotype was also associated with systemic features such as hematologic manifestations and Raynaud ' s phenomenon ( RP ) and with autoantibody production such as antinuclear , anti Ro ( SSA ) or La ( SSB ) autoantibodies and rheumatoid factor . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We also examined the spectrum of specific entities of antibodies that had previously been reported in association with LN in other series ( anti Smith ( Sm ) , RNP and SS A without anti SS B , anti SS A and SS B , anti RNP and Smith , anti RNP and dsDNA and anti RNP and SS A antibodies ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In contrast to the ssA subunit , the inhibin / activin ssB subunit was not detected in the four largest hierarchical follicles but was expressed in greatest abundance in the pool of F 6 F8 and small yellow follicles . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
She had suffered from keratoconjunctivitis sicca and xerostomia and had been diagnosed as having Sjgren ' s syndrome in February 1994 , based on histological examination of the minor salivary gland and sialography , and positivity for SS A and SS B antibody . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Immunisation of healthy mice with individual protein components of the La / Ro ribonucleoprotein ( RNP ) targeted in systemic lupus erythematosus and primary Sjgren ' s syndrome induced autoantibodies recognising Ro 60 ( SS A ) , Ro 52 ( SS A ) and La ( SS B ) and in some cases the molecular chaperones calreticulin and Grp 78 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Sera with different autoantibody specificities [ 45 anti Sm , 40 anti U1RNP , 40 anti Ro ( SSA ) / La ( SSB ) positive , 21 Antinuclear antibody positive , but negative for antibodies to extractable nuclear antigens ( ANA + / ENA ) and 75 normal human sera , ANA negative ] and 75 sera from patients with rheumatoid arthritis ( RA ) were tested for anti ( PPGMRPP ) 5 ( SOC ) 5 reactivity in order to evaluate the specificity and sensitivity of the method to detect anti Sm antibodies . ^^^ RNA immunoprecipitation assays for the detection of anti Sm and anti U1RNP antibodies and counter immunoelectrophoresis ( CIE ) for the detection of anti Ro ( SSA ) and anti La ( SSB ) antibodies were used as reference techniques . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Systemic lupus erythematosus ( SLE ) is an autoimmune disorder characterized by production of autoantibodies against intracellular antigens including DNA , ribosomal P , Ro ( SS A ) , La ( SS B ) , and the spliceosome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies SS A and SS B are frequently associated with complete heart block without structural heart defects . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The levels of expression of ssA , ssB , and follistatin mRNAs were determined in the pituitary gland on postnatal days ( PND ) 8 , 10 , 12 , 15 , and 21 by semiquantitative reverse transcription polymerase chain reaction . ^^^ All values were compared to those of adult females killed on diestrus . mRNA levels of subunit ssA were significantly ( p < 0 . 05 ) elevated on all postnatal days examined ; ssB mRNA levels were elevated above adult levels only on PND 10 ( p < 0 . 05 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The initiating antigens in SS remain unknown , but immune reactivity against SS A , SS B , fodrin , alpha amylase , and carbonic anhydrase have been demonstrated in patients with established disease . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Of 540 result pairs ( 90 sera tested for 6 ENAs [ Sm / RNP , Sm , SSA , SSB , Scl 70 , Jo 1 ] ) , 509 ( 94 % ) showed concordance . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The cytosolic 70 kDa heat shock proteins ( Hsp70s ) , Ssa and Ssb , of Saccharomyces cerevisiae are functionally distinct . ^^^ In addition , the ATPase activity of Hsp70s , like that of Ssa 1 , depends on the addition of K+ whereas Ssb activity does not . ^^^ Suprisingly , the isolated 44 kDa ATPase domain of Ssb has a Km and Vmax for ATP hydrolysis similar to those of Ssa , rather than those of full length Ssb . ^^^ Analysis of Ssa / Ssb fusion proteins demonstrates that the Ssb peptide binding domain fused to the Ssa ATPase domain generates an ATPase of relatively high activity and low steady state affinity for ATP similar to that of native Ssb . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Detection of anti Ro / SSA and anti La / SSB autoantibody producing cells in salivary glands from patients with Sjgren ' s syndrome . ^^^ OBJECTIVE : To investigate and identify the presence of cells producing anti Ro / SSA and anti La / SSB autoantibodies in salivary glands from patients with Sjgren ' s syndrome ( SS ) . ^^^ These findings indicate that anti Ro / SSA and anti La / SSB autoantibodies are produced and are present at sites of inflammation and indicate their potential involvement in the autoimmune exocrinopathy of this disease . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
INVESTIGATIONS : Biochemical tests showed a serum creatinine level of 6 . 1 mg / dl , a 1 : 5120 antinuclear antibody ( ANA ) titre , and positive values for Ro ( SS A ) and La ( SS B ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
METHODS : Autoantibody detection in 86 asymptomatic women was done blindly on a panel of 15 different antibodies ( dsDNA , ssDNA , histones , SSA / Ro , SSB / La , RNP , cardiolipin , phosphatidylserine , pyruvate dehydrogenase , Scl 70 , NC 1 , silicone , collagen 1 , 2 , and 4 ) . ^^^ Among the symptomatic group , the most common autoantibodies were histone ribosomal phosphate , SSA , SSB , Scl 70 , cardiolipin , phosphatidylserine , GM 2 ganglioside , and NC 1 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Ro ( SS A ) and La ( SS B ) antibodies were detected with similar frequency , and photosensitivity was not related to the presence of Ro antibody . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serological abnormalities ( extractable nuclear antigen [ ENA ] , SS A , SS B ) occurred in 20 % of patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti La and Ro ( SS A ) Ab responses were monitored following immunization of healthy mice with defined immunodominant and subdominant T cell determinants of the La ( SS B ) autoantigen synthesized as either monomeric or multiple antigenic ( MAP ) peptides . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
None of the HCV positive patients had detectable antibodies against SS A , SS B , RNP , Jo 1 , PCNA or Scl 70 , and the frequency of ANA / SMA / AMA was low . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We studied a total of 89 patients with chronic GVHD and 60 controls for AMA reactivity by ELISA and immunoblotting using recombinant PDC E 2 , BCOADC E 2 , and OGDC E 2 , immunoblotting of beef heart mitochondrial proteins , and reactivity to nuclei , smooth muscle ( ASMA ) , ribonucleoprotein JO 1 , extractable nuclear antigen , nuclear proteins SSA / SSB , ribonucleic P proteinase 3 , cardiolipin ( ACA ) , liver kidney microsomal , thyroid microsomal , myeloperoxidase , and the reactivity of rheumatoid factor . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Ssb and the ribosomal protein rpL 5 mRNAs were up regulated upon carbon upshift and down regulated upon amino acid limitation , unlike the mRNA of another cytosolic Hsp 70 , Ssa . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Six of seven had a positive antinuclear antibody ( ANA ) titer ranging from 1 : 40 to greater than 1 : 640 , with two patients having antibodies to Scl 70 and others to Sm , RNP , SS A ( Ro ) , or SS B ( La ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Saccharomyces cerevisiae contains distinct SSA and SSB gene families of cytosolic Hsp70s . ^^^ Elevated levels of SSA 1 but not SSB 1 suppressed the NLS GFP nuclear localization defects of nup 188 Delta cells . ^^^ Studies with Ssa1p / Ssb1p chimeras revealed that the Ssb1p NES is sufficient and necessary to inhibit the function of Ssa or Ssb type Hsp70s in nuclear transport . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Laboratory investigations showed hypergammaglobulinemia and high titers of autoantibodies such as RF , ANA , anti SS A and SS B antibody . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We studied the systemic manifestations of myasthenia gravis and its putative pathogenesis . 644 clinically , pharmacologically and electrophysiologically diagnosed patients with myasthenia gravis ( MG ) were studied . 33 patients had Graves disease , 5 periodic paralysis , 4 Hashimoto disease , and 4 epilepsy , polymyositis , rheumatoid arthritis and Guillain Barre syndrome each . 45 patients had positive anti skeletal muscle antibodies , 9 positive anti thyroid antibody , and 3 positive SSA and SSB . 11 MG patients with positive pyramidal signs ( MG+PS ) had no evidence of multiple sclerosis ( MS ) or other neurologic diseases . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We also found the relationship between HLA DR 52 and the anti antibodies of SSA or SSB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The five criteria measured at interview were : ( 1 ) the reporting of subjective oral symptoms lasting for > 3 months ; ( 2 ) the reporting of subjective ocular symptoms lasting for > 3 months ; ( 3 ) Schirmer 1 test ; ( 4 ) unstimulated salivary flow ; ( 5 ) autoantibodies [ Ro ( SS A ) , La ( SS B ) , rheumatoid factor ( RF ) , antinuclear antibodies ( ANA ) ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
One patient had ribosomal P antibodies , one had ribonuclear protein ( RNP ) antibodies and none had antibodies to Ro ( SSA ) or La ( SSB ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The risk of neonatal lupus ( skin eruptions , atrioventricular block ) is essentially related to the presence of anti Ro ( SSA ) and anti La ( SSB ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The patient had autoantibodies for SSa and SSb specific antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Here we demonstrate that another protein of the Hsp 70 family , Ssb , previously implicated in nascent polypeptide folding and protein turnover , exhibits effects on [ PSI ] which are opposite those of Ssa . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
They were evaluated for the clinical response , Systemic Lupus Activity Measure ( SLAM ) score before and after IVIg , levels of antinuclear antibody ( ANA ) , dsDNA ( double stranded DNA ) , SS A or SS B , ENA ( extractable nuclear antigens ) , C 3 and C 4 levels before and after the treatment , and before and after each treatment course . ^^^ These differences were found statistically significant only with respect to C 4 and SS A or SS B levels before IVIg courses . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Neonatal lupus erythematosus ( NLE ) is an inflammatory syndrome in the fetus or neonate associated with the presence of anti Ro ( SSA ) and anti La ( SSB ) antibodies in the mother . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To define Ro 52kD , Ro 60kD , and La specificities of autoantibodies within ANA negative sera , samples from 64 ANA negative but SSA positive patients undergoing investigation due to suspected CTD were analysed , using recombinant antigens and synthetic peptides by immunoblotting and ELISA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Modulation of the in vivo concentrations of the SSA and SSB proteins by deletion or overexpression affects HSF activity in a manner that is consistent with these findings and suggests the model that the SSA and SSB proteins perform distinct roles in the regulation of HSF activity . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The sera are now available to vendors and clinical laboratories for use in the detection of SS A , SS B , Sm , U 1 RNP , Scl 70 , Jo 1 , double stranded DNA , and centromere antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Annular erythema ( AE ) associated with anti Ro ( SSA ) and / or La ( SSB ) autoantibody in patients with Sjgren syndrome ( SS ) or with SS / systemic lupus erythematosus overlap syndrome ( SS / SLE ) , has recently been described in Orientals , and it may be a counterpart of annular skin lesion of the subacute cutaneous LE seen mostly in Caucasians . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Immune responses to SS A 52 kDa and 60 kDa proteins and to SS B 50 kDa protein in mothers of infants with neonatal lupus erythematosus . ^^^ Mothers who have anti SS A / Ro and anti SS B / La antibodies have a greater risk of bearing infants with neonatal lupus erythematosus ( NLE ) . ^^^ However , most mothers with anti SS A / Ro and anti SS B / La antibodies give birth to infants with no evidence of NLE . ^^^ We studied mothers who had the combination of clinical evidence of connective tissue disorder , positive antinuclear antibody and positive anti SS A / Ro and / or anti SS B / La antibodies . ^^^ We analysed SS A / Ro and SS B / La antibody responses to recombinant 60 , 52 and 50 kDa proteins in eight mothers of NLE infants and 19 mothers whose children did not have NLE . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Evaluation showed the presence of anti Ro ( SS A ) and anti La ( SS B ) autoantibody , with the presence of anti Ro ( SS A ) antibody against the 60 kd , but not the 52 kd , epitope , a pattern frequently seen in both the Asians with AE SS and in white patients with SCLE . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Laboratory data disclosed thrombocytopenia , hypergammaglobulinemia , and high titers of anti nuclear antibody , anti SS A , and anti SS B antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
SSA and SSB were obtained from spleen and nRNP / Sm complex from thymus . ^^^ Of 109 random SLE and Sjogren ' s syndrome sera 49 % and 35 % were positive , respectively , for human and bovine SSA , 26 % and 16 % for SSB . ^^^ The relative clinical sensitivity for all specimens identified as positive by human and / or bovine antigens was significantly higher with human than with bovine SSA , ( 93 % vs 67 % ; P < 0 . 001 , chi 2 ) , SSB ( 93 % vs 50 % ; P < 0 . 001 ) , and for nRNP / Sm ( 96 % vs 75 % ; P < 0 . 01 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Neonatal lupus erythematosus ( NLE ) is a rare disease characterized by the transplacental passage from the mother to the fetus of autoantibodies , in particular anti Ro ( SS A ) , anti La ( SS B ) , or both . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Sjgren ' s syndrome ( SS ) is a chronic autoimmune disorder of the exocrine glands of unknown aetiology , which is typically associated with focal lymphocytic infiltrates of glandular tissues and autoantibody responses against the Ro ( SSA ) and La ( SSB ) ribonucleoproteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
None of the 39 neonates from SLE mothers had neonatal lupus , anti Ro ( SSA ) or anti La ( SSB ) antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
These humoral autoimmune findings provide an unique opportunity to assess the pathogenic role of autoantibodies against the Ro ( SS A ) / La ( SS B ) complex , most notably for congenital heart block . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Endothelial response for the presence of chosen antinuclear antibodies , anti Ro ( SS A ) and anti La ( SS B ) and anti Sm in vasculitis against the background of existing lupus erythematosus ] . ^^^ The goal of the study was the evaluation of the role of the following nuclear antibodies : anti Ro ( SS A ) , anti La ( SS B ) and anti Sm in the endothelium damage process in the SLE patients with symptoms of microcirculation disturbances . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Endometrial expression of inhibin alpha , ssA and ssB subunits was examined by immunohistochemistry and in situ hybridization , across the menstrual cycle and in early pregnancy . ^^^ Following the onset of decidualization , expression of alpha , ssA and ssB subunits was up regulated in decidualized stromal cells . ^^^ A marked down regulation of alpha subunit was detected in glandular epithelium , whilst expression of ssA and ssB subunits was maintained . ^^^ Immunostaining was also observed for both ssA and ssB subunits in subpopulations of endometrial leukocytes , identified to be distinct subsets of macrophages , neutrophils and mast cells . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Finally , all patients were tested for the presence of autoantibodies , including : rheumatoid factor , antinuclear antibodies , antibodies to Ro ( SSA ) , La ( SSB ) nuclear antigens , anticardiolipin antibodies and antineutrophil cytoplasmatic antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We investigated the prevalence of symptoms of mucosal dryness , salivary gland pathology , lacrimal hyposecretion , and autoantibodies ( antinuclear antibody , SSA / SSB ) among patients diagnosed with CFS . ^^^ Antibody to nuclear antigen as well as anti La ( SSA ) and anti Ro ( SSB ) antibody were available for subjects with CFS . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In the typical forms , the biological signs associate a polyclonal hypergammaglobulinemia , sometimes a cryoblogulinemia , rheumatoid factor and anti SSA and anti SSB ANA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The following parameters were considered golden standard for the diagnosis of pSS : focus score > or = 2 foci / mm2 , double positivity for SSA and SSB antibodies , and a sialographic grade > or = 2 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
However , while a direct interaction between Sis 1 and Pab 1 is not detectable , both of these proteins physically interact with the essential Ssa ( and not Ssb ) family of hsp 70 proteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
BACKGROUND : The diagnosis of primary Sjgren ' s syndrome largely depends on pathological findings at lower lip biopsy , or the presence of anti SSA and / or anti SSB antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
No significant differences were found between the patients and the normal controls in the frequency of positive tests for antibodies to dsDNA , Sm , nRNP , SSA , SSB and Scl 70 antigens , or for parietal cell antibodies , anti neutrophil cytoplasmic antibodies ( ANCA ) , anti cardiolipin and anti thyroid antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Patients with positive rheumatoid factor ( RF ) , antinuclear antibodies ( ANA ) , or SSA or SSB antibodies had sialadenitis significantly more often than did patients with negative antibodies . ^^^ A significant association exists between focal sialadenitis and RF , ANA , SSA and SSB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Certain autoimmune disorders , including Sjgren syndrome ( SS ) and systemic lupus erythematosus ( SLE ) , are characterized by autoantibodies against the Ro / SSA and La / SSB cellular antigens . ^^^ Interestingly , the incidence of antibodies to SS 56 was associated with visceral complications in SLE , and roughly half of the 17 SS or SLE patients with no detectable antibodies to SSA and SSB antigens presented measurable antibodies against recombinant SS 56 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Histomorphometric analysis was performed for vessel area ( VA ) , stent strut area ( SSA ) , intimal area ( IA ) , and lumen area ( LA ) . ^^^ RESULTS : Injury scores , VA , SSA , and LA were similar among the control and radiated groups . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Because binding of Ssb to peptide substrates in vitro was undetectable , we assessed the importance of substrate binding using the chimera BAB , in which the peptide binding domain of Ssb is exchanged for the analogous domain of the more `` classical ' ' Hsp 70 , Ssa . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Crust normalized REE patterns indicated that SSA was enriched with Sc , Sm , Eu , Gd , Tb and slightly enriched with La , Ce ; IBA was enriched with Eu , Tb and slightly with La , Y , Ce ; FSA was slightly enriched with Sm , Eu , Tb ; REEs were not found to be elevated in HWA and AWA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Glomerulopathy occurred in 22 cases ( nephrotic syndrome 12 , glomerulonephritis 10 ) and mild renal failure ( RF ) was found in 14 . 69 . 1 % of the patients had hypergamma globulinemia . 64 . 3 % and 44 . 1 % of the patients showed positive anti SS A and anti SS B . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
A significant number of antigens from A , B , C , DR and DQ loci was investigated in 50 patients with primary Sjgren ' s syndrome with positive autoantibodies against dsDNA , ssDNA , Ro ( SS A ) , La ( SS B ) , RNP antigens , as well as with higher titer of anticardiolipin antibodies from IgM and IgG classes . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Intracellular trafficking and surface expression of SS A ( Ro ) , SS B ( La ) , poly ( ADP ribose ) polymerase and alpha fodrin autoantigens during apoptosis in human salivary gland cells induced by tumour necrosis factor alpha . ^^^ Autoantigens alpha fodrin , SS A ( Ro ) , SS B ( La ) , and the enzyme poly ( ADP ribose ) polymerase , were detected by conventional immunofluorescence and confirmed by Western immunoblotting . ^^^ At increasing times after apoptosis , nuclear proteins SS A ( Ro ) and SS B ( La ) , but not poly ( ADP ribose ) polymerase were relocated from the cell nucleus to the cell surface membrane . ^^^ These results show that autoantigens fodrin , SS A ( Ro ) and SS B ( La ) in human salivary gland cells undergo a striking redistribution during apoptosis and relocate to the cell membrane of apoptotic cells . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Snl1p bound specifically to the Ssa and Ssb forms of yeast cytosolic Hsp 70 , as revealed by two hybrid screening and co precipitations from yeast lysate . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Restricted specificity of intermolecular spreading to endogenous La ( SS B ) and 60 kDa Ro ( SS A ) in experimental autoimmunity . ^^^ Intermolecular spreading of humoral autoimmunity to different components of the Ro ( SS A ) and La ( SS B ) ribonucleoprotein ( RNP ) complex has been reported following immunization with a single component of the complex . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
CLINICAL PRESENTATION : Congenital heart block ( CHB ) in the absence of major structural abnormalities is associated with maternal antibodies to Ro ( SS A ) and La ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies binding the Ro ( or SSA ) and La ( or SBB ) proteins are commonly found in a high proportion of sera from patients with systemic lupus erythematosus or Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
RESULTS : Antibodies to dsDNA , the collagen like region of C1q , Sm , SSA , SSB , and chromatin were enriched in glomerular extracts , mainly from patients with proliferative lupus glomerulonephritis . ^^^ CONCLUSION : Autoantibodies with multiple different specificities form the immune deposits in glomeruli of patients with SLE , including antibodies to dsDNA , Sm , SSA , SSB , the collagen like region of C1q , and chromatin . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Laboratory data showed an elevated erythrocyte sedimentation rate , C reactive protein and CH 50 in addition to existing autoantibodies including anti nuclear antibody , anti SS A antibody , and anti SS B antibody . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Analysis of COBAS ANA response to particular antigenic specificities showed that SS B , Scl 70 and U1sn RNP specificities were saturating at high concentrations , whereas Jo 1 , SS A and nuclear and centromeric specificities exhibited lower responses . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
SSA IgG and SSB IgG autoantibodies were both present in one of these patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Regarding the laboratory findings , young women presented more often with anti Ro ( SSA ) and anti La ( SSB ) antibodies , while increased levels of erythrocyte sedimentation rate ( ESR ) were found in old women . ^^^ Using multiple logistic regression analysis , a statistically significant association of malar rash , discoid lesions , serositis , RP , anti Ro ( SSA ) / La ( SSB ) and increased ESR with sex was found independently of age , while only malar rash showed a statistically significant association with age independently of sex . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Furthermore , we also studied the association between xerostomia and xerophthalmia and serum auto antibodies ( anti nuclear , anti Ro ( SS A ) , anti La ( SS B ) ) and anti HCV antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Neonatal lupus erythematosus is an immune mediated disease associated with the transplacental passage of maternal autoantibodies , primarily anti Ro ( SS A ) and anti La ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Sjgren ' s syndrome was diagnosed based on microscopic findings of a labial salivary gland biopsy although anti SS A and anti SS B antibodies were negative . ^^^ The characteristics of our case were enlargement of lacrimal glands , the negativity of anti SS A and SS B antibodies , atypical onset and M proteinemia . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Isolated complete congenital heart block ( CCHB ) in a fetus is usually associated with the presence of autoantibodies to SSA ( Ro ) and SSB ( La ) antigens in the maternal circulation . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
LES , GS and PR can both be associated with anti SS A + / anti SS B antibodies linked to a risk of congenital auriculo ventricular block , which is fortunately low ( less than 5 % of the cases ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The patients ' sera were tested for anti nuclear ( ANA ) , as well as for nine different specific autoantibodies ( anti dsDNA , Sm , RNP , SSA , SSB , Scl 70 , centromere , Jo 1 and PM Scl ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Results of salivary scintigraphy were predicted to 51 % by ANA , SSA , SSB , IgG , IgA , diagnosis , vitality , and role limitations due to emotional problems . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Our study was done to evaluate the FIDIS Connective kit ( Biomedical Diagnostics , Marne la Valle , France ) for simultaneous quantitative determination in the same sample of 9 antinuclear antibody specificities directed against double stranded DNA , SSA , SSB , Sm , Sm / RNP , Scl 70 , Jo 1 , ribosome , and centromere B and to compare it with standardized commercial methods , enzyme immunoassay and immunofluorescence . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
METHODS : We synthesized recombinant centromere protein B , cytokeratin 19 , SSA 52 kDa antigen , SSA 60 kDa antigen , SSB antigen , and Jo 1 antigen and prepared anti nuclear antibody antigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Maternal antibodies to 52 kDa and 60 kDa SSA and 48 kDa SSB were determined by time resolved fluoroimmunoassay ( TR FIA ) and by immunoblotting . ^^^ RESULTS : Sixty of our 67 mothers ( 90 % ) of children with CHB had antibodies to SSA or SSB by the methods initially used in this study . ^^^ Increased risk of having a child with CHB was indicated by maternal primary SS and high levels of anti SSA and anti SSB by all assays , whereas low risk was indicated by maternal SLE or other CTD and undetectable or low levels of the antibodies . ^^^ No single anti SSA or anti SSB test was clearly superior to others , but in general , immunoblots were more specific than TR FIA . ^^^ Anti SSA and anti SSB are marker antibodies for mothers of children with CHB , and an increased risk of having an affected child is indicated by maternal primary SS and high titer antibodies to SSA and SSB . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Detection of anti SS A / Ro and anti SS B / La antibodies of IgA and IgG isotypes in saliva and sera of patients with Sjgren ' s syndrome . ^^^ To assess the frequency and the possibility of local production of autoantibodies against SS A / Ro and SS B / La in patients with primary Sjgren ' s syndrome ( SS ) , serum and saliva samples were obtained from 42 patients with SS , 10 with rheumatoid arthritis without sicca syndrome , and 12 healthy volunteers . ^^^ The frequencies of IgA anti SS A antibody , IgA anti SS B antibody , IgG anti SS A antibody and IgG anti SS B antibody in serum from SS patients were 45 % , 50 % , 43 % and 21 % , respectively . ^^^ The frequencies of IgA anti SS A antibody , IgA anti SS B antibody , IgG anti SS A antibody and IgG anti SS B antibody in saliva from SS patients were 31 % , 33 % , 40 % , and 19 % , respectively . ^^^ We also found secretory IgA anti SS A and anti SS B antibodies accompanying secretory components in saliva and sera in representative SS patients . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In 121 PAOD patients ( 61 + / 12 years ) with a less pronounced atherosclerotic risk profile , or rarefied distal arteries without sclerosis of the media , or elevated erythrocyte sedimentation rate ( ESR ) or C reactive protein ( CRP ) independent from the stage 4 of PAOD , the following autoantibodies were investigated : antinuclear antibodies ( ANA ) , antibodies against extractable nuclear antigens ( ENA ) : SCL 70 , RNP , SS A , SS B , Jo 1 , SM ) , double stranded DNA antibodies ( ds DNA ) , antineutrophil cytoplasmatic antibodies ( c and p ANCA ) , antiphospholipid antibodies [ phosphatidylserine ( APSA ) and beta ( 2 ) glycoprotein ] , smooth ( SMA ) and striated muscle ( StrMA ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti Ro / SSA and anti La / SSB autoantibodies in the tear fluid of patients with Sjgren ' s syndrome . ^^^ PURPOSE : To investigate the presence of anti Ro / SSA and anti La / SSB antibodies in the tear fluid and serum of patients with Sjgren ' s syndrome and to evaluate the association of these autoantibodies with the severity of keratoconjunctivitis sicca . ^^^ Evaluation of tear fluid and sera anti Ro / SSA and anti La / SSB levels was done by using a quantitative enzyme linked immunosorbent assay kit designed for the quantitative measurement of IgG class autoantibodies directed against highly purified SSA and SSB antigens . ^^^ RESULTS : Increased levels of anti Ro / SSA and anti La / SSB antibodies were detected in sera of 57 . 1 % and 50 % of SS patients , respectively . ^^^ A positive correlation was observed between serum and tear fluid titres of anti Ro / SSA ( r = 0 . 43 , p = 0 . 02 ) , but not of anti La / SSB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
A significant association of xerosis with anti SSA + SSB ( p = 0 . 033 ) antibodies was also demonstrated . ^^^ Annular erythema was found more often in primary ( 6 . 45 % ) than in secondary ( 3 . 2 % ) SS and was associated with SSA + SSB antibodies in 75 % of the cases . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
CHB detected in utero is strongly associated with maternal antibodies to SSA ( Ro ) and SSB ( La ) . ^^^ Congenital heart block associated with maternal anti SSA / SSB antibodies : a report of four cases ] . ^^^ Congenital heart block ( CHB ) associated with maternal anti SSA / SSB antibodies : a report of four cases . ^^^ It is very difficult to identify pregnant asymptomatic mothers carrying anti SSA / SSB antibodies . ^^^ We report four cases of infants born to asymptomatic mothers with anti SSA / SSB antibodies , three of them developed isolated congenital cardiac heart block and one with no evidence of CHB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
No reactivity with SSA , SSB , Sm , RNP , Jo 1 , Scl 70 , or ribosomal P antigens could be demonstrated in these eluates . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
He was diagnosed as having SS SAN because Schirmer test , Saxon test and both SS A and SS B antibodies were positive . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The prevalence of ANA , anti dsDNA , anti Sm , SSA , SSB and anti cardiolipin antibodies were studied in addition to complement C 3 and C 4 levels . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti SSA antibodies with concurrent SSB antibodies were associated with group 1 , whereas anti SSA antibodies with concurrent U ( 1 ) RNP antibodies were associated with group 3 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Results of the multiplexed assay were compared to results from established ENA enzyme linked immunosorbent assays ( ELISAs ) , and the agreement , sensitivity , and specificity , respectively , for the five ENA evaluated were as follows : SSA , 99 . 1 , 100 . 0 , and 98 . 8 % ; SSB , 98 . 6 , 88 . 9 , and 99 . 5 % ; Sm , 97 . 6 , 95 . 8 , and 97 . 9 % ; RNP , 97 . 2 , 92 . 7 , and 98 . 8 % ; Scl 70 , 93 . 6 , 50 . 0 , and 99 . 0 % . ^^^ In the 56 confirmed SLE patients , the frequency of significant concentrations of autoantibodies with the multiplexed assay was 21 . 4 % for SSA , 7 . 1 % for SSB , 10 . 7 % for Sm , 32 . 1 % for RNP , and 0 % for Scl 70 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Hsp 70 chaperones as modulators of prion life cycle : novel effects of Ssa and Ssb on the Saccharomyces cerevisiae prion [ PSI+ ] . [ PSI ( + ) ] is a prion isoform of the yeast release factor Sup 35 . ^^^ Experiments with chimeric constructs show that the peptide binding domain is a major determinant of differences in the effects of Ssa and Ssb proteins on [ PSI ( + ) ] . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
PURPOSE : To evaluate the performances and convenience of the Fidis analytical system ( BioMedical Diagnostics , Marnes la Vallee , France ) for the detection of nine autoantibodies associated with connective diseases : SS A , SS B , Sm , RNP , Scl 70 , Jo 1 , CENP B , P ribosomal and double stranded DNA antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Atrioventricular block , especially in the presence of maternal SS A and SS B autoantibodies , can be treated with Dexamethason and partly with Salbutamol , within international study protocols . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
CONCLUSION : Since the presence of autoantibodies against SS A and SS B are reported to accompany congenital heart block , the primary goal of therapy should be preventing this untoward effect of the disease . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Assessment of SS A and SS B in parotid saliva of patients with Sjgren ' s syndrome . ^^^ BACKGROUND : The purpose of this study was to compare the sensitivity of parotid saliva to that of serum in detecting anti SSA / Ro and anti SSB / La autoantibodies in patients with Sjgren ' s syndrome . ^^^ Serum and saliva samples were evaluated using AffiniTech SSA / Ro and SSB / La antibodies kits ( AffiniTech , Ltd . ^^^ The results were also compared with serological status of SS A and SS B as reported by an independent clinical laboratory . ^^^ RESULTS : Serum was significantly more sensitive than saliva in detecting SSA / Ro and SSB / La antibodies ( P = 0 . 001 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies against Sm are prevalent among low producer patients for both cytokines , a genotype not associated with lupus incidence , whereas low IL10 / high TNFalpha patients have the highest frequency of antibodies to SSa and SSb . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Both the infant and his mother were positive for anti Ro ( SS A ) and anti La ( SS B ) . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Among SLE patients , the GSTM 1 null genotype was associated with a lower frequency of hematological disorders ( P = 0 . 012 ) , and a higher SSA ( + ) / SSB ( ) autoantibody profile ( P = 0 . 042 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
These bar coded microparticles were used to tag and track key ANA markers : Jo 1 , Scl 70 , Sm , SmRNP , SSA , SSB , U1RNP , Centromere B , dsDNA , and a blank control microparticle . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Autoantibody frequencies detected by the Mikrogen , Innogenetics , and Imtec line assays were 14 . 0 % , 19 . 3 % , and 15 . 8 % for RNP ; 14 . 0 % , 22 . 8 % , and 14 . 0 % for Sm ; 26 . 3 % , 31 . 6 % , and 40 . 3 % for SSA ; 3 . 5 % , 12 . 3 % , and 14 . 0 % for SSB ; and 3 . 5 % , 14 . 0 % , and 10 . 5 % for histones . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Sera from systemic lupus erythematosus ( SLE ) patients ( n = 123 ) , systemic sclerosis patients ( n = 25 ) , and healthy controls ( n = 40 ) were analyzed for the presence of antibodies to RNP , Sm , SSA , SSB , CENP B , Scl 70 , and Jo 1 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The autoantibodies studied were : fluorescent ANA , anti double stranded DNA ( anti ds DNA ) , IgG and IgM anti cardiolipin ( ACA ) , anti histone H 1 and C ( H 1 , H2A , H2B , H 3 , H 4 ) , anti SSA , SSB , ENA , scleroderma 70 , thyroid peroxidase ( TPO ) and neutrophilic cytoplasmatic ( ANCA ) antibodies . ^^^ Levels of ANCA , anti ENA , SSA , SSB , RNP , scleroderma 70 and thyroid peroxidase ( TPO ) were negative in all patients and remained unchanged during the study . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Rowell ' s syndrome ( RS ) is a rare presentation of lupus erythematosus ( LE ) with erythema multiforme like lesions associated with antinuclear , anti La ( SS B ) / anti Ro ( SS A ) antibodies and rheumatoid factor ( RF ) positivity . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In yeast , these include the Hsp70s SSB and SSA as well as the Hsp 110 like Sse1 / 2p . ^^^ Both SSB and SSA Hsp70s form stable complexes with the Hsp 110 Sse1p . ^^^ Pulse chase analysis indicates that these Hsp70 / Hsp110 teams , SSB / SSE and SSA / SSE , transiently associate with newly synthesized polypeptides with different kinetics . ^^^ Notably , loss of SSB dramatically enhances the cotranslational association of SSA with nascent chains , suggesting SSA can partially fulfill an SSB like function . ^^^ On the other hand , the absence of SSE 1 enhances polypeptide binding to both SSB and SSA and impairs cell growth . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The yeast Hsp 110 Sse1 functionally interacts with the Hsp 70 chaperones Ssa and Ssb . ^^^ Through co immunoprecipitation analysis , we found that Sse 1 forms heterodimeric complexes with the abundant cytosolic Hsp70s Ssa and Ssb in vivo . ^^^ Binding of Ssa or Ssb to Sse 1 was mutually exclusive . ^^^ The ATPase domain of Sse 1 was found to be critical for interaction as inactivating point mutations severely reduced interaction with Ssa and Ssb . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We found 101 / 130 ( 78 % ) cases had at least one positive autoantibody test result [ antinuclear antibodies ( ANA ) , rheumatoid factor ( RF ) , SSA , SSB ] ; 78 / 123 ( 63 % ) had autoantibodies to SSA or SSB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Hybridoma 3G10 prepared by fusing splenocytes immunized with saikosaponin a BSA ( SSa BSA ) conjugate and a hypoxanthine aminopterin thymidine ( HAT ) sensitive mouse myeloma cell line , P 3 X63 Ag 8 U1 , secreted monoclonal antibodies with wide cross reactivity to saikosaponins including saikosaponin b ( 2 ) ( SSb ( 2 ) ) , c ( SSc ) and d ( SSd ) , which are stereo and / or regio isomers of SSa . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Two were positive for the SS A antibody , and the other was positive for the SS B antibody . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We performed neurological examinations and nerve conduction studies ( NCSs ) and measured serum antinuclear antibody ( ANA ) and SS A and SS B antibody levels . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
METHODS : One hundred and eighty five subjects ( female : male = 178 : 7 ) , who had at least one of the three autoantibodies , anti centromere ( ACA ) , anti Ro ( SSA ) , and / or anti La ( SSB ) , and 65 healthy females were enrolled . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
All patients were subjected to titration of the following autoantibodies by means of the immuno fluorimetry method : ANA , anti ENA ( SSA , SSB , SM , SM RNP , SCL 70 , Jo 1 ) , anti nDNA , anti histones . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serologic test results for antinuclear antibodies , including Sjgren antibodies anti Ro ( SS A ) and anti La ( SS B ) , were negative . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
We examined the colony morphology and chlamydospore production of 26 C . dubliniensis isolates and 100 C . albicans isolates on the following 5 proposed media : sesame seed agar ( SSA ) , rapeseed agar , canary grass seed agar , millet seed agar , and linseed agar ( LA ) . ^^^ The best results were obtained with SSA and LA because all 26 C . dubliniensis isolates showed rough colonies with peripheral hyphal fringes and abundant chlamydospores after 24 to 48 h of incubation at 25 degrees C . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
METHODS : Sera that demonstrated reactivity to 6 or more of the 8 antigens in an ALBIA kit ( QuantaPlex 8 : chromatin , Sm , RNP , Scl 70 , ribosomal P protein , SS A / Ro , SS B / La , Jo 1 ) were tested for autoantibodies by indirect immunofluorescence ( IIF ) on HEp 2 cell substrates , for IgG , IgM and IgA rheumatoid factor , chromatin and ribosomal P protein by ELISA and by LINE immunoassay ( LIA ) and immunoblotting ( IB ) . ^^^ Overall agreement of positive or negative tests between the ALBIA and IB , LIA and ELISA was 75 % for chromatin , 50 % for SS A , 27 . 5 % for Sm , 25 % for Rib P , 22 . 5 % for RNP , 20 % for Scl 70 , 15 % for Jo 1 and 7 . 5 % for SS B . 17 / 40 ( 42 . 5 % ) had a positive IgM , IgG or IgA rheumatoid factor , and 12 / 40 ( 30 % ) had all three isotype rheumatoid factors . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The IgG type of SS A / SS B autoantibodies were measured by nuclear immunoblot , developed by us , and the results were compared to other results obtained by anti SS A immunoblot and ELISA . ^^^ Altogether 33 out of 200 sera showed SS A and / or SS B bands in immunoblot , including all seven ANA Profile 3 ( Euroimmune ) positive sera . ^^^ Summing up , our study showed that the prevalence of SS A / SS B antibodies in a general random population might be higher than thought previously being detected in up to 16 . 5 % of persons including a significant number of those with HLA DR 3 or / and DR 11 alleles and with antibodies against enteroviruses . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Anti SS A and Anti SS B antibodies transferred from mothers have inflammatory and arrhythmogenic effects in the fetal conduction system . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The autoantigens SSA and SSB are translocated to the apoptotic blebs and trigger infiltrating B cells to produce autoantibodies against SSA and SSB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The human Y RNAs , small RNAs with an unknown function , are complexed with at least three proteins : the 60 , 000 M ( r ) Ro protein ( Ro 60 ) , the 52 , 000 M ( r ) Ro protein ( Ro 52 ) and the La protein ( La ) . ^^^ By immunodepletion of HeLa S 100 extracts for either Ro 60 , Ro 52 or La , followed by supplementation with recombinant Ro 60 or La , it was demonstrated that both Ro 60 and La bind to hY 1 RNA directly without being influenced by one of the other proteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The relative migration of Ro 52 , the 56K autoantigen and calreticulin increased with reduced acrylamide : bisacrylamide ratios in contrast to that of Ro 60 , La and Jo 1 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The possible pathological role of antibody subsets specific for different regions of 60 KD and 52 KD Ro / SSA proteins ( Ro 60 and Ro 52 ) and La protein is unclear . ^^^ Anti Ro 60 antibodies were more frequent in DR 3 positive patients and in DQ 1 negative patients whereas the presence of Ro 52 and La IgG antibodies was significantly increased in patients with A1 / B8 / DR3 haplotype . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To investigate if MRL / lpr mice produce the anti Ro / SS A and anti La / SS B autoantibodies common to Sjgren ' s syndrome patients , mouse sera were tested in ELISA and Western blot with recombinant Ro 60 , 000 MW , Ro 52 , 000 MW and La antigen . ^^^ Antibodies to Ro 60 , 000 MW and La were found in a low percentage of the older mice but not at all in the younger mice . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Microinjection of the protein components of human Ro RNPs , i . e . , La , Ro 60 , and Ro 52 , in 10 . laevis oocytes showed that all three proteins are able to enter the nucleus , albeit with different efficiencies . ^^^ Immunoprecipitation experiments using monospecific anti La and anti Ro 60 antibodies showed that the 10 . laevis La and Ro 60 homologues were cross reactive with the respective antibodies and that both 10 . laevis proteins were able to interact with human Y 1 RNA . ^^^ Further analyses indicated that : ( a ) association of 10 . laevis La and Ro 60 with Y RNAs most likely takes place in the nucleus ; ( b ) once formed , Ro RNPs are rapidly exported out of the nucleus ; and ( c ) the association with La is lost during or shortly after nuclear export . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In contrast to the Ro 52 autoantigen , Ro 60 and La autoantigens were mainly expressed in the nucleus of transfected cells under similar circumstances , indicating distinct localization of the intracellular pools of these autoantigens . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The interactions with Ro 60 and La differentially affect nuclear export of hY 1 RNA . ^^^ Ro RNPs are evolutionarily conserved ribonucleoprotein particles that consist of a small RNA , known as Y RNA , associated with several proteins , such as La , Ro 60 , and Ro 52 . ^^^ Injection of various hY 1 RNA mutants showed that an intact Ro 60 binding site is a prerequisite for nuclear export , whereas the presence of an intact La binding site resulted in strong nuclear retention of hY 1 RNA . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Patients with Sjgren ' s syndrome associated with nonprecipitating anti La antibodies represent a stable serological and clinical subset in which there appears to be limited diversification of the autoimmune response to the Ro 60 and La proteins of the Ro / La ribonucleoprotein . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Minor differences were detected when the frequencies of the Ro 60 kD , Ro 52 kD and La autoantibody specificities to full length proteins in the pSS and sSS groups were compared . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Using monospecific anti 60 kD Ro ( Ro 60 ) and anti La antibodies to retard RNPs containing these antigens during migration in the gels , the three major complexes were shown to contain Ro 60 ( C ) , La ( B ) , or both proteins ( A ) . ^^^ The specificity of RNA protein interactions in the reassembled complexes was further demonstrated using two 3 ' shortened hY 5 RNA transcripts lacking the La binding site ( hY 5 Alu 1 RNA ) and both the Ro 60 and La binding sites ( hY 5 Hha 1 RNA ) . hY 5 Hha 1 RNA still formed a single , minor complex when incubated with S 100 extract , suggesting interaction with a yet undefined protein . ^^^ Our assay may be used to detect low levels of antibodies to conformational determinants on Ro 60 and La proteins in human sera and antibody preparations . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In human cells , Ro RNPs consist of one of four small RNA molecules , termed hY 1 , hY 3 , hY 4 and hY 5 [ 35 ] , associated with several proteins , Ro 60 , Ro 52 and La [ 8 , 35 , 106 ] and possibly other , yet unidentified polypeptides . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
All sera were examined by Western blot using recombinant antigens , encoding the Ro 60 kD and the La proteins . ^^^ Six patients who reacted to all three antigens ( Ro 52 kD , Ro 60 kD and La ) had secondary Sjgren ' s syndrome . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To examine both possible correlations between anti Ro / SS A and anti La / SS B levels and their correlation with clinical disease activity in patients with Sjgren ' s syndrome ( SS ) or systemic lupus erythematosus ( SLE ) , an ELISA was developed using purified recombinant Ro 60 kDa , Ro 52 kDa and La antigens . ^^^ A temporal correlation of antibody levels to Ro and La antigens was found , and antibodies to different epitopes of the Ro 60 kDa protein showed parallel variation in seven of eight patients tested . ^^^ The results suggest that the serological response to Ro and La antigens , as well as to different epitopes of the Ro 60 kDa protein , is antigen driven and regulated by common mechanisms , and indicate a correlation of Ro and La antibodies with pathogenic events . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Other studies have shown that initiation of immunity to either Ro 52 or 60 kDaRo ( Ro 60 ) can lead to reciprocal spreading of autoimmunity to Ro 60 or Ro 52 , respectively , and induce anti La autoantibodies in some strains of mice . ^^^ To test the hypothesis that CR is physically associated with Ro 52 and / or Ro 60 we examined the sera of Ro 52 , Ro 60 and La immunized mice for intermolecular spreading to CR . ^^^ Immune sera from BALB / c and C3H / HeJ mice immunized with recombinant 6xHis mouse Ro 52 , 6xHis human Ro 60 or 6xHis human La were tested for reactivity by ELISA and immunoblotting with a full length human CR protein expressed as a soluble maltose binding protein fusion protein ( CR MBP ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Involvement of the Xenopus laevis Ro 60 autoantigen in the alternative interaction of La and CNBP proteins with the 5 ' UTR of L 4 ribosomal protein mRNA . ^^^ We also show that the Ro 60 autoantigen is involved in the common factor activity necessary for the binding of La and CNBP proteins to their respective sequences . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The role of polyclonal B cell activation was examined by measuring total gamma globulin as well as IgG reactive with other nuclear antigens including Ro 60 , Ro 52 and La , which although not a major component of the autoantibody responses in these mice , did show small but significant increases following immunization with M . bovis . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Immunizations with these peptides induced autoantibodies to Ro 60 , La , SmD , and 70 kD U 1 RNP without autoantibodies to Ro 52 , SmA , or SmB . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Immune responses to members of the heat shock protein hsp 70 and hsp 90 families were measured by immunoblotting and ELISA in sera from mice immunized and boosted with purified recombinant Ro 52 , Ro 60 and La ( SS B ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The Y RNA degradation products remain bound by the Ro 60 protein and in part also by the La protein , the only two proteins known to be stably associated with intact Ro ribonucleoprotein particles . ^^^ The size of the Y RNA degradation products is consistent with the protection from degradation of the most highly conserved region of the Y RNAs by the bound Ro 60 and La proteins . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the occurrence of IgA autoantibodies to Ro 52 kDa , Ro 60 kDa and La antigen in serum of patients with primary Sjgren ' s syndrome ( pSS ) and systemic lupus erythematosus ( SLE ) . ^^^ METHODS : Recombinant Ro 52 kDa , Ro 60 kDa and La antigens were used to analyse autoantibodies in serum from 25 patients with pSS , 30 patients with SLE and 20 controls using a semiquantitative immunoblotting approach . ^^^ RESULTS : Among the patients with pSS , 21 ( 84 % ) had detectable IgA autoantibodies to Ro 52 kDa , 13 ( 52 % ) to Ro 60 kDa and 20 ( 80 % ) to La antigen . ^^^ A comparison of several clinical features with the titres of IgA antibodies to Ro 52 kDa , Ro 60 kDa and La , revealed a significant relation between IgA anti Ro 52 and IgA anti La to sicca ( p < 0 . 05 ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
To build and try this system that we called RNP interaction trap assay ( RITA ) , we used as a model the autoantigenic Ro RNPs . hY RNAs bear distinct binding sites for Ro 60 and La proteins , and Ro RNPs are thus physiologically tripartite ( Ro60 / hY RNA / La ) . ^^^ Using recombinant La ( rLa ) and Ro 60 ( rRo 60 ) proteins and recombinant hY RNAs ( rhY ) co expressed in yeast , we found that RNPs made of rRo60 / rhY / rLa were readily reassembled . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The hY RNAs are a group of four small cytoplasmic RNAs of unknown function that are stably associated with at least two proteins , Ro 60 and La , to form Ro ribonucleoprotein complexes . ^^^ Furthermore , both anti Ro 60 and anti La antibodies coprecipitated hnRNP 1 , whereas coprecipitation of hnRNP K was not observed . ^^^ Taken together , these data strongly suggest that hnRNP 1 is a stable component of a subpopulation of Ro RNPs , whereas hnRNP K may be transiently bound or interact only with ( rare ) Y RNAs that are devoid of Ro 60 and La . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In this study an ELISA assay was used to quantify the levels of antibodies against the recombinant Ro 52 kD , Ro 60 kD , and La 48 kD proteins in plasma and saliva of 17 Sjgren ' s syndrome patients . ^^^ About one third of the patients had salivary enrichment of IgA and IgM against the Ro 52 kD , Ro 60 kD , and La 48 kD antigens and IgG against La 48 kD , while no enrichment of IgG against Ro 52 kD and Ro 60 kD was found . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Human Ro ribonucleoproteins ( RNPs ) are composed of one of the four small Y RNAs and at least two proteins , Ro 60 and La ; association of additional proteins including the Ro 52 protein and calreticulin has been suggested , but clear cut evidence is still lacking . ^^^ The majority of these complexes contained Ro 60 and La , whereas only a small proportion of Ro 52 appeared to be associated with Ro RNPs . ^^^ Coprecipitation of the 80 , 68 , 65 , and 53 kDa proteins by antibodies to Ro 60 and La was observed , suggesting that at least a proportion of the novel proteins may reside on the same particles as La and / or Ro 60 . ^^^ Interestingly , truncation of the La binding site resulted in decreased binding of the novel proteins ( but not of Ro 60 ) , indicating La to be required for efficient association . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The cellular locations of Ro 60 , Ro 52 , Sm , U 2 B ' ' and La were determined using monoclonal antibodies . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Affinity purified anti La Ab were reactive with Ro 60 , La , SmD , and 70 kDa U1RNP . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Several centers , using different techniques , have investigated the B cell epitopes on the protein components Ro 60 kD , Ro 52kD , and La 48 kD . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Autoantibodies against most relevant muscle specific ( Jo 1 , Mi 2 ) and non specific antigens ( PM Scl , U1RNP , native Ro , Ro 60 , Ro 52 , and La ) were detected with one or more detection techniques : counter immunoelectrophoresis , enzyme linked immunoassay , immunoblot and immunoprecipitation , each using different antigen preparations ( native , recombinant ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In human cells , Ro RNPs consist of one of four small RNA molecules , termed hY RNAs and at least two well characterized proteins , Ro 60 and La . ^^^ In previous Xenopus laevis oocyte microinjection studies , we showed that an intact Ro 60 binding site ( Stem loop 1 ) is a prerequisite for efficient nuclear export of hY 1 RNA , whereas an intact La binding site promotes nuclear retention ( Simons et al . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum samples from all patients were tested in a control laboratory for anti Ro / SSA and anti La / SSB autoantibodies by RNA precipitation assay and autoantibodies to the previously reported B cell linear epitopes of Ro 60 kDa ( p 169 190aa and p 211 232aa ) and La / SSB ( p 147 154aa , p 291 302aa , p 301 318aa , and p 349 364aa ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
In human cells , Ro RNPs consist of one of the four hY RNAs and two core proteins : Ro 60 and La . ^^^ To gain more insight into the composition and function of the Ro RNPs , we have immunopurified these particles from HeLa cell extracts using monoclonal antibodies against Ro 60 and La . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
METHODS : Sequential serum samples collected over 18 44 months from 18 anti Ro / SSA positive patients with systemic lupus erythematosus were analysed by ELISA with recombinant Ro 60 , Ro 52 and La antigens . ^^^ CONCLUSION : The Ro 60 , Ro 52 and La antibody profile is fixed at an early stage of disease and in most patients hardly changes . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Antibodies against recombinant Ro 60 Kd , Ro 52 and La 48 Kd proteins in primary Sjogren ' s Syndrome . ^^^ AIM : To determine the immune reactivity of sera from patients with Sjgren ' s syndrome , against fusion recombinant proteins ( prf ) Ro 60 Kd , Ro 52 Kd and La 48 Kd expressed in E coli and recombinant protein Ro 52 Kd , expressed in baculovirus ( prb ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Serum samples positive for ANAs were analysed by immunodiffusion and line immunoassay with recombinant SSA Ro 52 , natural SSA Ro 60 , and recombinant SSB . ^^^ Triple reactivity towards Ro 52 , Ro 60 , and SSB was primarily linked with SLE ( 55 . 8 % ) followed by pSS ( 20 . 9 % ) . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
METHODS : DNA and nucleosome antibodies were detected by ELISA , antibodies to SmB , U1A RNP , Ro 52 , Ro 60 and La by a new radioligand assay , using de novo synthesized radio labeled antigens . ^^^ RESULTS : Analysis of anti chromatin ( including anti nucleosome , anti dsDNA and anti histone antibodies ) and of anti snRNP antibodies ( including anti U1A RNP , anti SmB , anti Ro 52 , anti Ro 60 , anti La antibodies ) was performed in sequential sera from B / W , MRL+ / + , MRL Yaa and MRL lpr / lpr mice . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Previous studies showed that Ro 60 ( 316 335 ) induced Abs to Ro 60 , La , and snRNP proteins . ^^^ Absorption experiments showed that Abs to La and snRNP proteins were due to the induction of anti Ro 60 Abs cross reactive with these peptides . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Autoantigens were probed by indirect immunofluorescence and Western blot with autoantibodies or monoclonals against : DNA , Ro 60 , La , U 1 RNP , CENP B , DNA Topoisomerase 1 , Jo 1 and NuMA . ^^^ U 1 RNP , Jo 1 , DNA Topoisomerase 1 , CENP B and NuMA exhibited fragmentation and redistribution as a consequence of apoptosis ; in contrast , Ro 60 and La ribonucleoproteins did not show proteolysis . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
RESULTS : Five clusters of antibodies were defined by cluster analysis : 1 antibodies to SmB , SmD , RNP A , RNP C , and RNP 70k ; 2 antibodies to Ro 52 , Ro 60 , and SSB ; 3 , 4 , and 5 antibodies to ribosomal P , histones and dsDNA , respectively . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The serological pattern of autoantibodies to the Ro 52 , Ro 60 , and La 48 autoantigens in primary Sjgren ' s syndrome patients and healthy controls . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
Ro ribonucleoprotein ( RNP ) complexes are composed of one molecule of a small noncoding cytoplasmic RNA , termed Y RNA , and the two proteins Ro 60 and La . ^^^ Taken together , these data suggest that the Ro RNP components La , hnRNP K , and hnRNP 1 possess RNA chaperone activity , while Ro 60 Y RNA complexes might function as transporters , bringing other Y RNA binding proteins to their specific targets . . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
The sensitivity and specificity of testing for autoantibodies to Ro 52 , Ro 60 , and La were calculated for each set of criteria . ^^^ Antibodies to La but not to Ro 52 or Ro 60 were strongly correlated to internal organ ( kidney , lung , liver ) dysfunction in pSS ( OR 6 ; 95 % CI 3 12 ) , p < 0 . 0001 . ^^^
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA
Interacting proteins: P10155 and P05455	Pubmed	SVM Score :0.0
NA