| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| A mutant with a deletion of the central region ( residues 50 76 ) , which is conserved in the family members GADD45beta and GADD45gamma , lacked such activity . ^^^ Consistently , either GADD45beta or GADD45gamma bind to Cdc 2 in vivo . ^^^ However , unlike GADD 45 , neither GADD45beta nor GADD45gamma inhibited the Cdc 2 kinase or induced G ( 2 ) / M arrest . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| The MyD 118 ( Gadd45beta ) protein is a member of a family of structurally related proteins , including Gadd 45 ( Gadd45alpha ) and CR 6 ( Gadd45gamma ) , that have critical roles in regulating growth arrest and apoptosis . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Moreover , Gadd45a can complex with the two other members of the Gadd 45 family of stress induced proteins , human Gadd45b ( MyD 118 ) and Gadd45g ( CR 6 ) . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Importantly , mice with compound deficiency in Gadd45beta and Gadd45gamma spontaneously developed signs of autoimmune lymphoproliferative syndrome and systemic lupus erythematosus . ^^^ Our findings therefore identify the Gadd45beta / Gadd45gamma mediated control of effector autoimmune lymphocytes as an attractive novel target for autoimmune disease therapy . . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Gadd45a ( Gadd 45 ) , Gadd45b ( MyD 118 ) , and Gadd45g ( CR 6 ) constitute a family of evolutionarily conserved , small , acidic , nuclear proteins , which have been implicated in terminal differentiation , growth suppression , and apoptosis . ^^^ GADD45b and GADD45g are cdc2 / cyclinB1 kinase inhibitors with a role in S and G2 / M cell cycle checkpoints induced by genotoxic stress . ^^^ Yet , whether Gadd45b and / or Gadd45g function as inhibitors of cdc2 / cyclinB1 kinase and / or play a role in G2 / M cell cycle arrest has not been fully established . ^^^ In this work , we show that Gadd45b and Gadd45g specifically interact with the Cdk1 / CyclinB1 complex , but not with other Cdk / Cyclin complexes , in vitro and in vivo . ^^^ Data also has been obtained that Gadd45b and Gadd45g , as well as GADD45a , interact with both Cdk 1 and cyclinB 1 , resulting in inhibition of the kinase activity of the Cdk1 / cyclinB1 complex . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| GADD45gamma is induced more strongly ( 7 fold ) than GADD45beta ( 3 fold ) and GADD45alpha ( 2 fold ) . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Gadd45g / CR6 , Gadd45b / MyD118 , and Gadd45a / Gadd45 are members of a gene family that displays distinct patterns of gene expression in response to stimuli that induce differentiation , growth arrest , and / or apoptosis . ^^^ A comparison of the putative transcription factor binding sites found in the sequences of the gene family members suggests , that like Gadd45b , NF kappaB and STATs may be responsible for the differences in regulation of expression observed between Gadd45g and Gadd45a . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| GADD45beta / GADD45gamma and MEKK 4 comprise a genetic pathway mediating STAT 4 independent IFNgamma production in T cells . ^^^ The stress inducible molecules GADD45beta and GADD45gamma have been implicated in regulating IFNgamma production in CD 4 T cells . ^^^ Expression of GADD45beta or GADD45gamma promotes IFNgamma production in MEKK4+ / + T cells , but not in MEKK 4 / cells or in cells treated with a p 38 inhibitor . ^^^ Thus , MEKK 4 mediates the action of GADD45beta and GADD45gamma on p 38 activation and IFNgamma production . ^^^ During Th 1 differentiation , the GADD45beta / GADD45gamma / MEKK4 pathway appears to integrate upstream signals transduced by both T cell receptor and IL12 / STAT4 , leading to augmented IFNgamma production in a process independent of STAT4 . . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Cell cycle and mitotic arrest is in agreement with up regulation of NF 2 , ETS 2 , CLU , GADD45alpha , GADD45beta , and GADD45gamma and down regulation of AURKB , TOP2A , CCNA , CCNB , PRC 1 , BUB 1 , NuSAP , IFI 16 , and BRCA 1 . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Gadd45a , gadd45b and gadd45g ( Gadd45 / MyD118 / CR6 ) are genes that are rapidly induced by genotoxic stress . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Acutely elevating NaCl ( and / or urea ) reduces Gadd45a , but increases Gadd45b and Gadd45g mRNA , depending on the mix of NaCl and urea and the rate of increase of osmolality . ^^^ Nevertheless , p2mIME cells from Gadd45b ( / ) , Gadd45g ( / ) , and Gadd45bg ( / ) mice survive elevation of NaCl ( or urea ) essentially the same as do wild type cells . p 53 ( / ) Cells do not tolerate as high a concentration of NaCl ( or urea ) as p 53 ( + / + ) cells , but urinary concentrating ability of p 53 ( / ) mice is normal , as is the histology of inner medullas from p 53 ( / ) and Gadd45abg ( / ) mice . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Gadd45a , Gadd45b , and Gadd45g ( Gadd45 / MyD118 / CR6 ) are genes that are rapidly induced by genotoxic stress and have been implicated in genotoxic stress induced responses , notably in apoptosis . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Using a yeast two hybrid method , three related proteins , GADD45alpha ( = GADD 45 ) , GADD 45 , ( = MyD 118 ) , and GADD45gamma , were identified that bound to an N terminal domain of MTK 1 . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| CR 6 : A third member in the MyD 118 and Gadd 45 gene family which functions in negative growth control . ^^^ Analysis of CR 6 expression during hematopoietic cell development associated with growth arrest and apoptotic cell death , upon exposure of hematopoietic cells to a variety of growth arrest and apoptotic stimuli , and in a variety of murine tissues , has revealed that CR 6 expression differs significantly from the expression of the related MyD 118 and Gadd 45 genes . ^^^ Nevertheless , CR 6 , like MyD 118 and Gadd 45 , suppressed colony formation of human lung carcinoma H 1299 cells . ^^^ These data suggest that CR 6 plays similar , but not identical , roles to MyD 118 and Gadd 45 in negative control of cell growth . . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning , expression , and mapping of a novel human cDNA , GRP 17 , highly homologous to human gadd 45 and murine MyD 118 . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Cytokine response gene # 6 ( CR 6 ) , cloned from interleukin 2 stimulated T lymphocytes , is homologous to GADD 45 and MyD 118 , genes which promote cell cycle arrest and apoptosis . ^^^ By comparison , when CR 6 , GADD 45 , and MyD 118 genes were expressed ectopically in proliferating pRb+ cells , either G1 / S or G2 / M transition was effectively blocked , so that there was no endoreduplication . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Gadd 45 family genes encode nuclear acidic proteins composed of Gadd 45 , MyD 118 , and CR 6 . ^^^ Sequence analysis showed that Gadd 45 family proteins ( Gadd 45 , MyD 118 , and CR 6 ) contain LXXLL signature motifs considered necessary and sufficient for the binding of several coactivators to nuclear receptors . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| In addition , GADD 45 inhibition of Cdc 2 kinase activity was compared with Myd 118 and CR 6 , two other members of the GADD 45 family . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| GADD 45 , MyD 118 , and CR 6 ( also termed GADD45alpha , beta , and gamma ) comprise a family of genes that encode for related proteins playing important roles in negative growth control , including growth suppression . ^^^ The MyD 118 , CR 6 , and GADD 45 proteins were shown to predominantly localize within the cell nucleus . ^^^ Moreover , it is shown that CR 6 interacts with PCNA via a domain that also mediates interaction of both GADD 45 and MyD 118 with PCNA . ^^^ Importantly , evidence has been obtained that interaction of CR 6 with PCNA impedes the function of this protein in negative growth control , similar to observations reported for MyD 118 and GADD45 . . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| Thus , to further explore the effects of these genes on cell growth and cell viability , either in the presence or absence of extrinsic stress , we have established M 1 myeloblastic leukemia and H 1299 lung carcinoma cell lines , where high level ectopic expression of MyD 118 , Gadd 45 , or CR 6 can be induced by isopropyl beta D thiogalactopyranoside ( IPTG ) . ^^^ By taking advantage of these cell lines , it was observed that in the absence of genotoxic stress , inducible expression of MyD 118 , Gadd 45 and / or CR 6 resulted in retardation of cellular proliferation and accumulation of cells in the G 1 phase of the cell cycle . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| The PCNA gene is induced by p 53 , while PCNA protein interacts with p 53 controlled proteins Gadd 45 , MyD 118 , CR 6 and , most importantly , p 21 , in the process of deciding cell fate . ^^^ |
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| Interacting proteins: O75293 and O95257 |
Pubmed |
SVM Score :0.0 |
| NA |
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