| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| SPA mainly induced IgG PFC and only a few IgA and IgM PFC . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.71324612 |
| To determine whether this complement activation 1 ) was due to an interaction between VH3+ Igs and the Fab binding site of SpA and 2 ) proceeded via the classical complement pathway , we tested a panel of monoclonal IgM proteins for the ability to hind C1q following interaction with SpA . 0.71324612^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Two dimensional polyacrylamide gel electrophoresis analysis of cryoglobulins and identification of an IgM associated peptide . ^^^ N terminal microsequencing ( 12 amino acid residues ) revealed that this IgM associated peptide was an unknown protein . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| In 1993 , we reported the presence of an IgM associated peptide ( M ( r ) 44 kDa ; pI 5 . 45 ) in all immunoglobulin M ( IgM ) fractions purified from plasma / serum by various methods . ^^^ To gain insight into the nature of this peptide , we further analyzed , using modern proteomic tools , the IgM associated peptide isolated from cryoglobulins . ^^^ Theses sequences together with the already known N terminal sequence allowed us to identity the IgM associated peptide as Sp alpha ( O 43866 in TrEMBL database ; CD 5 antigen like ) . ^^^ IgM are associated to Sp alpha ( CD 5 antigen like ) . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| However , in the control group , averaged results from ELISAs that identify SP A and that identify IgM antibodies to SP A or to SP B , C showed significant differences between pretreatment sera and sera obtained at 1 week of age . ^^^ Positive ELISA values were more frequently found in the control group than in the surfactant treated group with regard to SP A , and IgM against SP A and SP B , C in sera from neonates at 1 week of age . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Electrophoresis and immunoblotting analysis demonstrated that the fraction isolated from serum of a patient with PAP or IPF by anti SP A immunoaffinity column chromatography consisted chiefly of human IgG and IgM , and that it also contained SP A . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Here we are able to show that ( 1 ) autoreactivity to SP A , as expressed by IgG and IgM autoantibodies , is present in synovial fluid ( SF ) isolated from patients with rheumatoid arthritis ( RA ) ; ( 2 ) in absorption experiments only a limited degree of cross reactivity between autoantibodies reactive with C1q and SP A is observed ; ( 3 ) there is no cross reactivity between autoantibodies reactive with type 2 collagen ( CII ) and those reactive with SP A or C1q ; ( 4 ) autoantibodies react with polymeric ( dimers and larger ) SP A , but not with monomeric SP A subunits , indicating that a degree of quaternary structure is required for antibody binding . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| The degradative enzyme was purified by anion exchange chromatography and identified by ion trap mass spectroscopy as Pseudomonas elastase , which was shown to directly degrade SP A and SP D . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| METHODS : The concentrations of SP A , SP D , CRP , IgA , IgM , IgG , and rheumatoid factor were measured in the synovial fluid of 7 patients with rheumatoid arthritis and 3 samples of healthy synovial fluid obtained at autopsy . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| If , before the addition of MNL , the antibody treated , infected monolayers were incubated with either IgM rheumatoid factor ( RF ) , staphylococcal protein A ( SPA ) , or anti Fc gamma serum , antibody dependent cell mediated cytolysis ( ADCC ) was markedly depressed . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Furthermore , IgM produced by both sets of clones exhibited a similar frequency of VH 3 heavy chain subgroup expression , as determined by reactivity with staphylococcal protein A ( SpA ) and VH 3 associated CRI expression ( B 6 and / or D 12 ) . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| In contrast , the `` alternative binding site ' ' of Staphylococcal Protein A ( SPA ) represents an unconventional determinant , because ; ( 1 ) SPA is bound by a large proportion of non immune IgM , IgA and IgG F ( ab ' ) 2 , ( 2 ) SPA is bound only to Fab from the VH 3 family , which can be encoded by at least four different germline genes , ( 3 ) SPA binding is independent of VL usage , ( 4 ) by flow cytometry SPA is bound by > 15 % of tonsilar B cells , but not to T cells . ( 5 ) In vitro stimulation with an SPA containing mitogen induces the preferentially production of Ig bearing a VH 3 marker . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Introduction of 5 , 10 and 25 micrograms SpA at the time of the primary antigenic challenge inhibited both the primary IgM and the secondary IgM and IgG responses in a dose dependent manner . ^^^ Administration of 5 and 10 micrograms SpA at the time of the secondary antigenic challenge enabled the host to maintain the otherwise depressed secondary IgM response equivalent to the normal primary response . ^^^ In contrast , 25 micrograms SpA at the time of the secondary antigenic challenge inhibited both the IgM and IgG PFC responses . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| It was also demonstrated that Staphylococcus aureus protein A ( SpA ) , which has recently been shown to bind to the F ( ab ) region of VHIII molecules , could block the interaction of some B 6 CRI positive IgM to the anti CRI . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Supernatants were tested for IgG and IgM RBC AuAbs by use of 125I staphylococcal protein A ( SPA ) . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Using polyclonal chicken antibodies against SPA , we have detected a crossreactive determinant shared by human RFs from different individuals , but not by non RF IgM and IgG . ^^^ Chicken anti SPA was shown to bind to 18 of 19 IgM RFs and 2 of 2 IgG RFs isolated from different individuals . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Staphylococcal protein A ( SPA ) is a bacterial membrane protein which has distinct binding sites for Fc gamma and for the Fab region of some IgM , IgG , IgA , and IgE molecules . ^^^ Binding of 24 isolated human monoclonal IgM proteins to SPA was measured in a solid phase RIA . ^^^ Binding to SPA was seen with 10 of 11 VHIII IgM , but none of the 7 VHI or 6 VHII . ^^^ Similarly , polyclonal IgM fractionated on a SPA Sepharose CL4B column showed nearly complete partition of VHIII molecules into the SPA binding fraction , and VHI and VHII subgroup proteins into the fall through . ^^^ We conclude that SPA binding is a functional marker for VHIII H chains in human IgM molecules . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| A radioimmunoassay using cardiolipin as antigen and labelled SPA , anti human IgG , anti human IgM , anti human IgA as second antibodies in detecting anti cardiolipin antibody with the sera from 308 patients and 70 normal controls . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| In addition , the above sera and some anti toxoplasma seropositive human sera ( with IFAT antibody titers greater than or equal to 1 : 16 ) were adsorbed with staphylococcal protein A ( SpA ) and assayed for changes in IgM and IgG antibody titers so as to evaluate the usefulness of SpA adsorption in detecting anti toxoplasma IgM which shows up early in toxoplasma infections . ^^^ After SpA adsorption , however , the IgM IFAT antibody could be detected in a few specimens as early as 2 days post infection . ^^^ Even after SpA treatment , only about half of the 16 day samples showed IgM antibody titers and the other specimens still remained negative . ^^^ In general , IgM antibody titers increased 2 6 fold after SpA adsorption while IgG antibodies were almost completely removed after the treatment with a residual IgG IFAT titer of less than or equal to 1 : 4 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Staphylococcal protein A ( SPA ) is a bacterial membrane protein that possesses , in addition to its Fc gamma binding activity , a distinct specificity for the Fab region of some IgM , IgA , IgG , and IgE . ^^^ In a previous study of human monoclonal and polyclonal IgM , we demonstrated that binding to SPA was highly restricted to molecules of the VHIII subgroup , and that nearly all VHIII IgM were able to bind SPA . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Specificity of the serologic results of SPA treated infant sera correlated with IgM specific identification of the causative agent . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| The protein A binding site of human IgM was studied by affinity chromatography on SpA Sepharose using fragments derived from a human monoclonal SpA reactive IgM , Iz . ^^^ On the other hand mild reduction of IgM Iz to monomeric subunits results in a dramatic loss of SpA affinity . ^^^ Thus , the principal requirement for SpA reactivity with IgM Iz seems to be related to the presence of Fabmu regions in a polymeric state resembling native IgM . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Staphylococcal protein A ( SPA ) has been used to remove maternal IgG from cord or neonatal sera to make serologic testing for congenital infection more IgM specific . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| The S aureus Cowan strain 1 bound canine IgG , IgM , and IgA , whereas binding was not observed with the SPA negative Wood 46 strain of S aureus . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| A monoclonal IgM , isolated from the serum of a patient with CLL , whose lymphocytes were able to form rosettes with SpA HRBC and to proliferate in vitro after stimulation with Cowan Staph , also showed a marked inhibitory activity on the Cowan Staph induced proliferation or normal non T cells . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Initial studies in young subjects demonstrated that a Staph protein A ( SpA ) driven system could simultaneously assess the proliferative and differentiative capabilities of B cells resulting in IgM production . ^^^ Although B cells from elderly subjects continuously exposed to SpA displayed proliferative responses equal to young subjects , the differentiation of B cells from elderly subjects into IgM ISC was markedly reduced as compared to young subjects . ^^^ Thus , B cells from certain elderly humans have intrinsic impairments of differentiation required for optimal IgM production even though activation and proliferation remain normal in the presence of SpA . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| The immunoglobulin site ( s ) that mediates the alternative mechanism of interaction between immunoglobulins and staphylococcal protein A ( SpA ) was studied by using a monoclonal human IgM . ^^^ Several IgM fragments were tested for their inhibitory effect in a competitive binding assay of 125I IgM to SpA . ^^^ Furthermore , antibodies specific for the VH domain completely inhibited the SpA IgM interaction . ^^^ These results indicate that the alternative SpA binding site of IgM is located in VH regions . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| On the other hand , IgM was not usually observed in NP 1 , but it was usually found in linear pattern on the surface of STr in SpA . ^^^ IgG and IgM in the sera gave no significant difference between SpA and NP 1 . ^^^ The results lead us to the conclusion that humoral immune cytotoxicity by IgM in cooperation with C may be involved in the mechanisms responsible for the damage of Tr in SpA . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Nearly one third of the B cell clones from both sources produced IgM reactive with staphylococcal protein A ( SPA ) . ^^^ All of 22 B cell clones producing IgM reactive with SPA expressed VH 3 genes , while none of the control 15 clones secreting IgM nonreactive with SPA expressed VH 3 . ^^^ The IgM proteins reactive with SPA could be clearly divided into two subjects based on the differential binding avidity to solid phase SPA . ^^^ Both kappa and lambda light chains were used in each subset of SPA reactive IgM . ^^^ The high frequencies of B cells committed to the production of SPA reactive IgM in normal blood and the restricted use of VH 3 heavy chain genes in nearly germline configuration in these cells support the notion that SPA behaves like a superantigen toward human B cells . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Human peripheral blood B cells stimulated with anti CD 3 activated T cells or the Wood 46 strain of Staphylococcus aureus , which lacks SPA , expressed IgM containing all VH families , as detected by reverse transcription PCR . ^^^ In contrast , stimulation with SPA containing S . aureus or SPA Sepharose resulted in biased expression of VH 3 containing IgM . ^^^ Similarly , cord blood B cells that require costimulation for Ig production induced by anti CD 3 activated T cells produced only VH 3 containing IgM on costimulation with SPA containing S . aureus . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| However , the bacterial membrane protein , Staphylococcal protein A ( SpA ) , has sites that interact with the Fab of many IgM , IgA , IgG , and IgE , and in recent reports we have provided evidence of VH restriction in Fab that bind SpA . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Five cold agglutinin fractions contained large amounts of VH 3 encoded IgM ( compared to pooled normal IgM ) by virtue of their binding to modified protein Staph A ( SPA ) , and absorption of three CA fractions with modified SPA specifically removed anti i / I binding specificity entirely . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Binding to SPA in ELISA occurred with 15 of 15 VH 3 IgM , but none of 12 IgM from the VH 1 , VH 4 , VH 5 , or VH 6 families . ^^^ A comparison of the corresponding VH protein sequences , and those of previously studied IgM , identified a probable site for SPA binding that includes VH 3 residues in framework region 3 ( FR 3 ) , and perhaps FR 1 and 3 ' complementary determining region 2 . ^^^ The results thus demonstrate that among human IgM , specificity for SPA is encoded by at least 11 different VH 3 germline genes . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| To assess the participation of IgM and IgG in alloreactivity , sera were treated with dithiothreitol ( DTT ) or Staphylococcus aureus protein A ( SpA ) , respectively . ^^^ Our results show that DTT and SpA treatment are technically simple and reproducible methods of discriminating between IgG and IgM alloantibodies and can be recommended for routine use in clinical analysis of sera from hypersensitized renal patients awaiting kidney graft . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Human IgM that express the variable region genes of the VH 3 family bind staphylococcal protein A ( SPA ) . ^^^ We previously reported that the SPA binding IgM can be divided into two groups based on the differential binding avidities for solid phase SPA . ^^^ The nucleotide sequences of the expressed Ig heavy chain genes were determined on 20 B cell clones that produce SPA binding IgM . ^^^ The germ line VH 3 gene usage in IgM with high avidities for SPA were distinct from the germ line VH 3 genes used in IgM with low avidities for SPA . ^^^ There was no correlation in the usage of D or JH genes or in the usage of light chains in IgM according to the SPA binding avidity . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| The antigen biding activity of IgM NAb increased after isolation of the serum immunoglobulins on a Staphylococcus Protein A ( SPA ) Sepharose column . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| The results confirmed that both molecules are bound by most 5 ( H ) 3 IgM , but pFv is also recognized by 5 ( H ) 3 and 5 ( H ) 6 Ig that do not interact with SpA . ^^^ For both SpA and pFv , binding is less frequent among IgG than IgM . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| High serum immunoglobulin M ( IgM ) and IgG responses to SPA were induced by subcutaneous immunization with SPA bovine serum albumin in Freund adjuvant . ^^^ Serum and salivary IgA responses to SPA in addition to IgM and IgG responses were induced by intranasal immunization with the conjugate and cholera toxin B subunit . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Site directed mutagenesis of a single amino acid residue in CDR 2 converted an IgM rheumatoid factor which did not bind SPA to an SPA binder . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| However , the binding ability to IgA and IgM showed a marked difference between the various SpA derived proteins of different compositions . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Detection of telomerase activity by combination of TRAP method and scintillation proximity assay ( SPA ) . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| To that effect , we used a new combination of TRAP assay and SPA , which provides reproducible data for the quantitation and detection of variations in enzyme activity . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Spa exposure correlated with disease ( P= . 001 ) and IgM seropositivity ( P= . 007 ) . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To evaluate the clinical value of the determination of the complement components C ' 3c and C ' 4 and immunoglobulines IgG , IgA and IgM synovial fluid concentrations with regard to pathophysiology and currently used RA and SpA classification criteria . ^^^ SF IgG , IgA and IgM as well as the calculated SF / S ratios were within the same range in RA and SpA fluids . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Three of the 6 anti dsDNA positive SpA patients had IgM and IgA anti dsDNA antibodies , and 2 had IgM anti dsDNA antibodies alone . ^^^ CONCLUSION : Infliximab treatment may induce ANAs , and especially IgM and IgA anti dsDNA antibodies , in RA and SpA patients . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| On the other hand , when the results obtained by our ELISA system were compared with those of an imported measles virus IgM ELISA kit ( EIAgen ; Adaltis Italia SPa , Bologna , Italy ) , a high level of agreement was shown ( k = 0 . 926 ) . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Because the most recent reports indicated a much greater reactivity of purified SpA with canine immunoglobulins than was suggested by our observations , we quantitatively reevaluated the binding of canine IgG and IgM to Cowan 1 strain SpA under the conditions of use in our laboratory . ^^^ The purified IgG and IgM were assessed for SpA reactivity by affinity chromatography using a SpA agarose column . ^^^ The relative proportions of total chromatographed IgG or IgM in the flow through ( SpA nonbindable ) and eluate ( SpA bindable ) fractions were determined by absorbance at 280 nm . ^^^ The IgG and IgM in each immunoglobulin fraction were also nonspecifically adsorbed to microtitration plates and tested for reactivity with 125I SpA using a solid phase immunoradiometric assay ( IRMA ) . ^^^ Approximately 18 % of the affinity purified canine IgG and 33 % of the affinity purified canine IgM did not bind to the SpA affinity column and were also unreactive with 125I SpA using the IRMA . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| To understand the molecular basis for this activity , we have solved the crystal structure of the complex between domain D of SpA and the Fab fragment of a human IgM antibody to 2 . 7 A resolution . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Microspheres containing , on average , less than one IgG per contact area were held in the optical trap while an SpA coated substrate was scanned beneath them at a distance of approximately 50 nm . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| The presence of Spalpha was ascertained as an IgM associated protein in the serum cryoprecipitate from a patient with HCV infection . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| These data indicate that Spalpha is a circulating protein that may play a role in the homeostasis of IgM antibodies . . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| Comparison of the respective affinities of the two Ig demonstrated that IgM kappa Germ restores its SpA binding capacity , and shows a moderate decrease in its affinity for tubulin . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| METHODS : Levels of HC gp 39 mRNA were detected by reverse transcription polymerase chain reaction in PBMC of 31 patients with RA , 6 with osteoarthritis ( OA ) , 10 with spondylarthropathy ( SpA ) , 5 with systemic lupus erythematosus ( SLE ) , and of 10 healthy controls . ^^^ RESULTS : HC gp 39 mRNA expression in PBMC was increased in RA patients ( the HC gp 39 / tubulin ratio was 0 . 8690 + / 0 . 5240 ) , compared with OA ( P = 0 . 024 ) , SpA ( P = 0 . 049 ) , SLE ( P = 0 . 043 ) and with healthy control subjects ( P = 0 . 033 ) . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| SpA synovitis was characterized by higher vascularity and infiltration with CD163+ macrophages and polymorphonuclear leukocytes ( PMNs ) and by lower values for lining layer hyperplasia , lymphoid aggregates , CD1a+ cells , intracellular citrullinated proteins , and MHC HC gp 39 complexes than RA synovitis . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| In RA , 44 % of samples exhibited positive staining for intracellular citrullinated proteins and 46 % for MHC HC gp 39 peptide complexes , whereas no staining for these markers was observed in SpA samples . ^^^ In contrast , vascularity ( P < 0 . 001 ) and neutrophils were increased in PsA as compared with RA ( P = 0 . 010 ) , whereas staining for intracellular citrullinated proteins and MHC HC gp 39 peptide complexes was exclusively observed in RA ( both P = 0 . 001 ) , indicating that the same discriminating features are found in PsA and other SpA subtypes compared with RA . ^^^ |
|
| Interacting proteins: O43866 and P29965 |
Pubmed |
SVM Score :0.0 |
| PROCEDURE : After optimization of platelet collection and assay conditions , concentrations of PSAIg were measured , using radiolabeled staphylococcal protein A ( SpA ) and polyclonal antibodies against canine IgG ( anti gamma ) and IgM ( anti micro ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.71324612 |
| To determine whether this complement activation 1 ) was due to an interaction between VH3+ Igs and the Fab binding site of SpA and 2 ) proceeded via the classical complement pathway , we tested a panel of monoclonal IgM proteins for the ability to hind C1q following interaction with SpA . 0.71324612^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Detection of telomerase activity by combination of TRAP method and scintillation proximity assay ( SPA ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| To that effect , we used a new combination of TRAP assay and SPA , which provides reproducible data for the quantitation and detection of variations in enzyme activity . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Microspheres containing , on average , less than one IgG per contact area were held in the optical trap while an SpA coated substrate was scanned beneath them at a distance of approximately 50 nm . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| The degradative enzyme was purified by anion exchange chromatography and identified by ion trap mass spectroscopy as Pseudomonas elastase , which was shown to directly degrade SP A and SP D . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| If , before the addition of MNL , the antibody treated , infected monolayers were incubated with either IgM rheumatoid factor ( RF ) , staphylococcal protein A ( SPA ) , or anti Fc gamma serum , antibody dependent cell mediated cytolysis ( ADCC ) was markedly depressed . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Furthermore , IgM produced by both sets of clones exhibited a similar frequency of VH 3 heavy chain subgroup expression , as determined by reactivity with staphylococcal protein A ( SpA ) and VH 3 associated CRI expression ( B 6 and / or D 12 ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| In contrast , the `` alternative binding site ' ' of Staphylococcal Protein A ( SPA ) represents an unconventional determinant , because ; ( 1 ) SPA is bound by a large proportion of non immune IgM , IgA and IgG F ( ab ' ) 2 , ( 2 ) SPA is bound only to Fab from the VH 3 family , which can be encoded by at least four different germline genes , ( 3 ) SPA binding is independent of VL usage , ( 4 ) by flow cytometry SPA is bound by > 15 % of tonsilar B cells , but not to T cells . ( 5 ) In vitro stimulation with an SPA containing mitogen induces the preferentially production of Ig bearing a VH 3 marker . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Introduction of 5 , 10 and 25 micrograms SpA at the time of the primary antigenic challenge inhibited both the primary IgM and the secondary IgM and IgG responses in a dose dependent manner . ^^^ Administration of 5 and 10 micrograms SpA at the time of the secondary antigenic challenge enabled the host to maintain the otherwise depressed secondary IgM response equivalent to the normal primary response . ^^^ In contrast , 25 micrograms SpA at the time of the secondary antigenic challenge inhibited both the IgM and IgG PFC responses . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Staphylococcal protein A ( SPA ) is a bacterial membrane protein that possesses , in addition to its Fc gamma binding activity , a distinct specificity for the Fab region of some IgM , IgA , IgG , and IgE . ^^^ In a previous study of human monoclonal and polyclonal IgM , we demonstrated that binding to SPA was highly restricted to molecules of the VHIII subgroup , and that nearly all VHIII IgM were able to bind SPA . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| However , in the control group , averaged results from ELISAs that identify SP A and that identify IgM antibodies to SP A or to SP B , C showed significant differences between pretreatment sera and sera obtained at 1 week of age . ^^^ Positive ELISA values were more frequently found in the control group than in the surfactant treated group with regard to SP A , and IgM against SP A and SP B , C in sera from neonates at 1 week of age . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| It was also demonstrated that Staphylococcus aureus protein A ( SpA ) , which has recently been shown to bind to the F ( ab ) region of VHIII molecules , could block the interaction of some B 6 CRI positive IgM to the anti CRI . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Supernatants were tested for IgG and IgM RBC AuAbs by use of 125I staphylococcal protein A ( SPA ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Using polyclonal chicken antibodies against SPA , we have detected a crossreactive determinant shared by human RFs from different individuals , but not by non RF IgM and IgG . ^^^ Chicken anti SPA was shown to bind to 18 of 19 IgM RFs and 2 of 2 IgG RFs isolated from different individuals . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| In addition , the above sera and some anti toxoplasma seropositive human sera ( with IFAT antibody titers greater than or equal to 1 : 16 ) were adsorbed with staphylococcal protein A ( SpA ) and assayed for changes in IgM and IgG antibody titers so as to evaluate the usefulness of SpA adsorption in detecting anti toxoplasma IgM which shows up early in toxoplasma infections . ^^^ After SpA adsorption , however , the IgM IFAT antibody could be detected in a few specimens as early as 2 days post infection . ^^^ Even after SpA treatment , only about half of the 16 day samples showed IgM antibody titers and the other specimens still remained negative . ^^^ In general , IgM antibody titers increased 2 6 fold after SpA adsorption while IgG antibodies were almost completely removed after the treatment with a residual IgG IFAT titer of less than or equal to 1 : 4 . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| The immunoglobulin site ( s ) that mediates the alternative mechanism of interaction between immunoglobulins and staphylococcal protein A ( SpA ) was studied by using a monoclonal human IgM . ^^^ Several IgM fragments were tested for their inhibitory effect in a competitive binding assay of 125I IgM to SpA . ^^^ Furthermore , antibodies specific for the VH domain completely inhibited the SpA IgM interaction . ^^^ These results indicate that the alternative SpA binding site of IgM is located in VH regions . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Specificity of the serologic results of SPA treated infant sera correlated with IgM specific identification of the causative agent . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| The protein A binding site of human IgM was studied by affinity chromatography on SpA Sepharose using fragments derived from a human monoclonal SpA reactive IgM , Iz . ^^^ On the other hand mild reduction of IgM Iz to monomeric subunits results in a dramatic loss of SpA affinity . ^^^ Thus , the principal requirement for SpA reactivity with IgM Iz seems to be related to the presence of Fabmu regions in a polymeric state resembling native IgM . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Staphylococcal protein A ( SPA ) is a bacterial membrane protein which has distinct binding sites for Fc gamma and for the Fab region of some IgM , IgG , IgA , and IgE molecules . ^^^ Binding of 24 isolated human monoclonal IgM proteins to SPA was measured in a solid phase RIA . ^^^ Binding to SPA was seen with 10 of 11 VHIII IgM , but none of the 7 VHI or 6 VHII . ^^^ Similarly , polyclonal IgM fractionated on a SPA Sepharose CL4B column showed nearly complete partition of VHIII molecules into the SPA binding fraction , and VHI and VHII subgroup proteins into the fall through . ^^^ We conclude that SPA binding is a functional marker for VHIII H chains in human IgM molecules . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| A radioimmunoassay using cardiolipin as antigen and labelled SPA , anti human IgG , anti human IgM , anti human IgA as second antibodies in detecting anti cardiolipin antibody with the sera from 308 patients and 70 normal controls . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| SPA mainly induced IgG PFC and only a few IgA and IgM PFC . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Initial studies in young subjects demonstrated that a Staph protein A ( SpA ) driven system could simultaneously assess the proliferative and differentiative capabilities of B cells resulting in IgM production . ^^^ Although B cells from elderly subjects continuously exposed to SpA displayed proliferative responses equal to young subjects , the differentiation of B cells from elderly subjects into IgM ISC was markedly reduced as compared to young subjects . ^^^ Thus , B cells from certain elderly humans have intrinsic impairments of differentiation required for optimal IgM production even though activation and proliferation remain normal in the presence of SpA . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| To assess the participation of IgM and IgG in alloreactivity , sera were treated with dithiothreitol ( DTT ) or Staphylococcus aureus protein A ( SpA ) , respectively . ^^^ Our results show that DTT and SpA treatment are technically simple and reproducible methods of discriminating between IgG and IgM alloantibodies and can be recommended for routine use in clinical analysis of sera from hypersensitized renal patients awaiting kidney graft . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Two dimensional polyacrylamide gel electrophoresis analysis of cryoglobulins and identification of an IgM associated peptide . ^^^ N terminal microsequencing ( 12 amino acid residues ) revealed that this IgM associated peptide was an unknown protein . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Nearly one third of the B cell clones from both sources produced IgM reactive with staphylococcal protein A ( SPA ) . ^^^ All of 22 B cell clones producing IgM reactive with SPA expressed VH 3 genes , while none of the control 15 clones secreting IgM nonreactive with SPA expressed VH 3 . ^^^ The IgM proteins reactive with SPA could be clearly divided into two subjects based on the differential binding avidity to solid phase SPA . ^^^ Both kappa and lambda light chains were used in each subset of SPA reactive IgM . ^^^ The high frequencies of B cells committed to the production of SPA reactive IgM in normal blood and the restricted use of VH 3 heavy chain genes in nearly germline configuration in these cells support the notion that SPA behaves like a superantigen toward human B cells . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Staphylococcal protein A ( SPA ) has been used to remove maternal IgG from cord or neonatal sera to make serologic testing for congenital infection more IgM specific . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| The S aureus Cowan strain 1 bound canine IgG , IgM , and IgA , whereas binding was not observed with the SPA negative Wood 46 strain of S aureus . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| A monoclonal IgM , isolated from the serum of a patient with CLL , whose lymphocytes were able to form rosettes with SpA HRBC and to proliferate in vitro after stimulation with Cowan Staph , also showed a marked inhibitory activity on the Cowan Staph induced proliferation or normal non T cells . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| On the other hand , IgM was not usually observed in NP 1 , but it was usually found in linear pattern on the surface of STr in SpA . ^^^ IgG and IgM in the sera gave no significant difference between SpA and NP 1 . ^^^ The results lead us to the conclusion that humoral immune cytotoxicity by IgM in cooperation with C may be involved in the mechanisms responsible for the damage of Tr in SpA . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Binding to SPA in ELISA occurred with 15 of 15 VH 3 IgM , but none of 12 IgM from the VH 1 , VH 4 , VH 5 , or VH 6 families . ^^^ A comparison of the corresponding VH protein sequences , and those of previously studied IgM , identified a probable site for SPA binding that includes VH 3 residues in framework region 3 ( FR 3 ) , and perhaps FR 1 and 3 ' complementary determining region 2 . ^^^ The results thus demonstrate that among human IgM , specificity for SPA is encoded by at least 11 different VH 3 germline genes . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Site directed mutagenesis of a single amino acid residue in CDR 2 converted an IgM rheumatoid factor which did not bind SPA to an SPA binder . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| However , the binding ability to IgA and IgM showed a marked difference between the various SpA derived proteins of different compositions . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Electrophoresis and immunoblotting analysis demonstrated that the fraction isolated from serum of a patient with PAP or IPF by anti SP A immunoaffinity column chromatography consisted chiefly of human IgG and IgM , and that it also contained SP A . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Human IgM that express the variable region genes of the VH 3 family bind staphylococcal protein A ( SPA ) . ^^^ We previously reported that the SPA binding IgM can be divided into two groups based on the differential binding avidities for solid phase SPA . ^^^ The nucleotide sequences of the expressed Ig heavy chain genes were determined on 20 B cell clones that produce SPA binding IgM . ^^^ The germ line VH 3 gene usage in IgM with high avidities for SPA were distinct from the germ line VH 3 genes used in IgM with low avidities for SPA . ^^^ There was no correlation in the usage of D or JH genes or in the usage of light chains in IgM according to the SPA binding avidity . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Human peripheral blood B cells stimulated with anti CD 3 activated T cells or the Wood 46 strain of Staphylococcus aureus , which lacks SPA , expressed IgM containing all VH families , as detected by reverse transcription PCR . ^^^ In contrast , stimulation with SPA containing S . aureus or SPA Sepharose resulted in biased expression of VH 3 containing IgM . ^^^ Similarly , cord blood B cells that require costimulation for Ig production induced by anti CD 3 activated T cells produced only VH 3 containing IgM on costimulation with SPA containing S . aureus . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| However , the bacterial membrane protein , Staphylococcal protein A ( SpA ) , has sites that interact with the Fab of many IgM , IgA , IgG , and IgE , and in recent reports we have provided evidence of VH restriction in Fab that bind SpA . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Five cold agglutinin fractions contained large amounts of VH 3 encoded IgM ( compared to pooled normal IgM ) by virtue of their binding to modified protein Staph A ( SPA ) , and absorption of three CA fractions with modified SPA specifically removed anti i / I binding specificity entirely . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| To understand the molecular basis for this activity , we have solved the crystal structure of the complex between domain D of SpA and the Fab fragment of a human IgM antibody to 2 . 7 A resolution . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Spa exposure correlated with disease ( P= . 001 ) and IgM seropositivity ( P= . 007 ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| The antigen biding activity of IgM NAb increased after isolation of the serum immunoglobulins on a Staphylococcus Protein A ( SPA ) Sepharose column . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| The results confirmed that both molecules are bound by most 5 ( H ) 3 IgM , but pFv is also recognized by 5 ( H ) 3 and 5 ( H ) 6 Ig that do not interact with SpA . ^^^ For both SpA and pFv , binding is less frequent among IgG than IgM . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| High serum immunoglobulin M ( IgM ) and IgG responses to SPA were induced by subcutaneous immunization with SPA bovine serum albumin in Freund adjuvant . ^^^ Serum and salivary IgA responses to SPA in addition to IgM and IgG responses were induced by intranasal immunization with the conjugate and cholera toxin B subunit . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Because the most recent reports indicated a much greater reactivity of purified SpA with canine immunoglobulins than was suggested by our observations , we quantitatively reevaluated the binding of canine IgG and IgM to Cowan 1 strain SpA under the conditions of use in our laboratory . ^^^ The purified IgG and IgM were assessed for SpA reactivity by affinity chromatography using a SpA agarose column . ^^^ The relative proportions of total chromatographed IgG or IgM in the flow through ( SpA nonbindable ) and eluate ( SpA bindable ) fractions were determined by absorbance at 280 nm . ^^^ The IgG and IgM in each immunoglobulin fraction were also nonspecifically adsorbed to microtitration plates and tested for reactivity with 125I SpA using a solid phase immunoradiometric assay ( IRMA ) . ^^^ Approximately 18 % of the affinity purified canine IgG and 33 % of the affinity purified canine IgM did not bind to the SpA affinity column and were also unreactive with 125I SpA using the IRMA . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Here we are able to show that ( 1 ) autoreactivity to SP A , as expressed by IgG and IgM autoantibodies , is present in synovial fluid ( SF ) isolated from patients with rheumatoid arthritis ( RA ) ; ( 2 ) in absorption experiments only a limited degree of cross reactivity between autoantibodies reactive with C1q and SP A is observed ; ( 3 ) there is no cross reactivity between autoantibodies reactive with type 2 collagen ( CII ) and those reactive with SP A or C1q ; ( 4 ) autoantibodies react with polymeric ( dimers and larger ) SP A , but not with monomeric SP A subunits , indicating that a degree of quaternary structure is required for antibody binding . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| PROCEDURE : After optimization of platelet collection and assay conditions , concentrations of PSAIg were measured , using radiolabeled staphylococcal protein A ( SpA ) and polyclonal antibodies against canine IgG ( anti gamma ) and IgM ( anti micro ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| METHODS : The concentrations of SP A , SP D , CRP , IgA , IgM , IgG , and rheumatoid factor were measured in the synovial fluid of 7 patients with rheumatoid arthritis and 3 samples of healthy synovial fluid obtained at autopsy . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To evaluate the clinical value of the determination of the complement components C ' 3c and C ' 4 and immunoglobulines IgG , IgA and IgM synovial fluid concentrations with regard to pathophysiology and currently used RA and SpA classification criteria . ^^^ SF IgG , IgA and IgM as well as the calculated SF / S ratios were within the same range in RA and SpA fluids . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| In 1993 , we reported the presence of an IgM associated peptide ( M ( r ) 44 kDa ; pI 5 . 45 ) in all immunoglobulin M ( IgM ) fractions purified from plasma / serum by various methods . ^^^ To gain insight into the nature of this peptide , we further analyzed , using modern proteomic tools , the IgM associated peptide isolated from cryoglobulins . ^^^ Theses sequences together with the already known N terminal sequence allowed us to identity the IgM associated peptide as Sp alpha ( O 43866 in TrEMBL database ; CD 5 antigen like ) . ^^^ IgM are associated to Sp alpha ( CD 5 antigen like ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Three of the 6 anti dsDNA positive SpA patients had IgM and IgA anti dsDNA antibodies , and 2 had IgM anti dsDNA antibodies alone . ^^^ CONCLUSION : Infliximab treatment may induce ANAs , and especially IgM and IgA anti dsDNA antibodies , in RA and SpA patients . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| On the other hand , when the results obtained by our ELISA system were compared with those of an imported measles virus IgM ELISA kit ( EIAgen ; Adaltis Italia SPa , Bologna , Italy ) , a high level of agreement was shown ( k = 0 . 926 ) . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| The presence of Spalpha was ascertained as an IgM associated protein in the serum cryoprecipitate from a patient with HCV infection . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| These data indicate that Spalpha is a circulating protein that may play a role in the homeostasis of IgM antibodies . . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| Comparison of the respective affinities of the two Ig demonstrated that IgM kappa Germ restores its SpA binding capacity , and shows a moderate decrease in its affinity for tubulin . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| METHODS : Levels of HC gp 39 mRNA were detected by reverse transcription polymerase chain reaction in PBMC of 31 patients with RA , 6 with osteoarthritis ( OA ) , 10 with spondylarthropathy ( SpA ) , 5 with systemic lupus erythematosus ( SLE ) , and of 10 healthy controls . ^^^ RESULTS : HC gp 39 mRNA expression in PBMC was increased in RA patients ( the HC gp 39 / tubulin ratio was 0 . 8690 + / 0 . 5240 ) , compared with OA ( P = 0 . 024 ) , SpA ( P = 0 . 049 ) , SLE ( P = 0 . 043 ) and with healthy control subjects ( P = 0 . 033 ) . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| SpA synovitis was characterized by higher vascularity and infiltration with CD163+ macrophages and polymorphonuclear leukocytes ( PMNs ) and by lower values for lining layer hyperplasia , lymphoid aggregates , CD1a+ cells , intracellular citrullinated proteins , and MHC HC gp 39 complexes than RA synovitis . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| In RA , 44 % of samples exhibited positive staining for intracellular citrullinated proteins and 46 % for MHC HC gp 39 peptide complexes , whereas no staining for these markers was observed in SpA samples . ^^^ In contrast , vascularity ( P < 0 . 001 ) and neutrophils were increased in PsA as compared with RA ( P = 0 . 010 ) , whereas staining for intracellular citrullinated proteins and MHC HC gp 39 peptide complexes was exclusively observed in RA ( both P = 0 . 001 ) , indicating that the same discriminating features are found in PsA and other SpA subtypes compared with RA . ^^^ |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P29965 and O43866 |
Pubmed |
SVM Score :0.0 |
| NA |
|