| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.92000268 |
| By electrophoretic mobility gel shift assay ( EMSA ) and methylation interference assay , GKLF was found to bind BTE in a manner similar to several other transcription factors known to interact with BTE including Sp 1 and BTEB . 0.92000268^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| In co transfection experiments , GKLF transactivated a reporter gene linked to the GKLF 1 kb 5 ' flanking region , as did Sp 1 , Sp 3 and Cdx 2 . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| The region upstream of the transcription start site is characterized by the presence of a TATA box at bases 32 / 26 , DNA binding elements for transcription factors c Myc , N Myc , Sp 1 and nuclear factor kappaB as well as putative activator protein ( AP ) 1 , cAMP response element binding protein ( CREB ) , hepatocyte nuclear factor ( HNF ) 1 , HNF 3 , TGT 3 , gut enriched Krppel factor ( GKLF ) , thyroid transcription factor ( TTF ) 1 and glucocorticoid receptor element ( GRE ) binding sites . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| The tissue dependent keratin 19 gene transcription is regulated by GKLF / KLF4 and Sp 1 . ^^^ This activity was mapped to a short region containing an overlapping binding site for gut enriched Krppel like factor ( GKLF / KLF4 ) and Sp 1 . ^^^ GKLF has a higher binding affinity and is the predominant binding factor in cells with low Sp 1 protein levels . ^^^ Pancreatic acinar cells normally do not express K 19 , but overexpression of GKLF and Sp 1 in these cells leads to aberrant expression , similar to what is observed in pancreatic cancer . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Sequential deletion and site directed mutation analysis of the CD 1 promoter have identified the sequence between 141 and 66 , a region containing an Sp 1 response element , to be essential for GKLF function . ^^^ By electrophoretic mobility gel shift assay , recombinant GKLF and nuclear extracts from HT 29 cells were found to bind to the Sp 1 motif on the CD 1 promoter . ^^^ The inhibitory effect of GKLF on the CD 1 promoter activity was completely abolished by excessive amount of Sp 1 DNA and GKLF significantly reduced the stimulatory function of Sp 1 suggesting that GKLF and Sp 1 may compete for the same binding site on the CD 1 promoter . ^^^ These results indicate that GKLF is a transcriptional repressor of the CD 1 gene and that the inhibitory effect of GKLF is , in part , mediated by interaction with the Sp 1 binding domain on its promoter . . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| The 1 . 7 and 1 . 2 bp of 5 ' flanking regions isolated from each gene , respectively , contain Sp 1 and gut enriched Kruppel like factor ( GKLF ) binding sites , but otherwise show little identity . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Transfection studies using reporter constructs and chromatin immunoprecipitation assays also demonstrated that GKLF inhibited transactivation of the ODC gene by interfering with the binding of Sp 1 to the ODC promoter . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| In vitro studies demonstrated that mutation of specific cis elements in the SLC19A2 minimal promoter [ Gut enriched Krupple like factor ( GKLF ) , nuclear factor 1 ( NF 1 ) , and stimulating protein 1 ( SP 1 ) ] led to a decrease in activity . ^^^ The interacting factors were determined by oligonucleotide competition and antibody supershift analysis and shown to be GKLF , NF 1 , and SP 1 . ^^^ The results demonstrate the importance of GKLF , NF 1 , and SP 1 in regulating the activity of the SLC19A2 promoter and provide direct in vivo confirmation of promoter activity . . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| An interplay of Sp 1 , GKLF and CREB 2 controls human Pre alpha Inhibitor gene ( ITIH 3 ) transcription . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| This idea is supported by recent studies showing that the p 53 dependent activation of several genes in human and mouse cells requires some additional transcription factors , such as Sp 1 , GKLF , Ets 1 , and IRF 1 . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Binding of GKLF / GKLFa , Sp 1 , and TIEG 1 to the CD11d promoter in vivo is dependent on their zinc finger DNA binding domains . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Kruppel like factor 4 ( KLF 4 ) represses histidine decarboxylase gene expression through an upstream Sp 1 site and downstream gastrin responsive elements . ^^^ We further showed that KLF 4 inhibits HDC promoter activity by competing with Sp 1 at the upstream GC box and also independently by binding the three downstream gastrin responsive elements . ^^^ Taken together , these results indicate that KLF 4 can act to repress HDC gene expression by Sp 1 dependent and independent mechanisms . . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Synergistic activation of the rat laminin gamma 1 chain promoter by the gut enriched Kruppel like factor ( GKLF / KLF4 ) and Sp 1 . ^^^ The activity of GKLF is dependent on a synergism with another Kruppel like factor , Sp 1 . ^^^ The LAMC 1 promoter appears to have multiple GKLF and Sp 1 responsive elements which may account for the synergistic activation . ^^^ We provide evidence that the synergistic action of GKLF and Sp 1 is dependent on the promoter context and the integrity of GKLF activation and DNA binding domain . ^^^ Thus , the Sp 1 GKLF synergistic activation of the LAMC 1 promoter may be one of the avenues for expression of laminin gamma 1 chain when laminin is needed to regulate cell differentiation . . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Moreover , transactivation of KLF 4 by butyrate appears to be mediated through interaction with a Sp 1 binding domain on the promoter and is also likely to involve histone acetylation . . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Activation of the human pregnancy specific glycoprotein PSG 5 promoter by KLF 4 and Sp 1 . ^^^ Furthermore , we used previous data showing the binding of Sp 1 transcription factor to a GT box ( 443 / 437 ) and co transfection assays with KLF 4 and Sp 1 to demonstrate the strong synergic activity of these two factors on the PSG 5 promoter . . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| Sp 1 consensus sequences in the PKG 1 promoter were required for Rho regulation and bound nuclear proteins in a cell density dependent manner , including the Krppel like factor 4 ( KLF 4 ) . ^^^ KLF 4 was identified as a major trans acting factor at two proximal Sp 1 sites ; active RhoA suppressed KLF 4 DNA binding and trans activation potential on the PKG 1 promoter . ^^^ |
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| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43474 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
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