Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.64956871
In comparison with HDAC 1 and HDAC 2 , HDAC 3 remains relatively uncharacterized , and very few proteins have been shown to interact with HDAC 3 . 0.64956871^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The use of high dose cytosine arabinoside ( HDAC 3 gm / m2 ) appears rational based on cytosine pharmacology . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
This suppression can be rescued by ectopic expression of HDAC 3 at early stage . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
HDAC 3 and 4 both demonstrated protein protein interactions with sNAC 1 and lNAC 1 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
MAb HU / Ch2 7 ( IgG ) reacted strongly with HD 3 and IV3NeuGc alpha nLc4Cer ( HD 5 ) and weakly with VI3NeuGc alpha nLc6Cer ( HD 7 ) and 4 O acetyl HD 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
This monoclonal antibody reacted strongly with N glycolylneuraminyl alpha 2 3 lactosylceramide ( HD 3 ) and slightly with N glycolylneuraminyl alpha 2 3 lactoneotetraosylceramide ( HD 5 ) , but did not react with N glycolylneuraminyl alpha 2 3 lactoneohexaosylceramide ( HD 7 ) , N acetylneuraminyl alpha 2 3 lactosylceramide ( GM 3 ) and other derivatives of HD 3 prepared by chemical modification of the sialic acid residue of HD 3 , which indicates that the monoclonal antibody is directed precisely toward the terminal sialic acid and whole structure of HD3 . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Differential display cloning of a novel human histone deacetylase ( HDAC 3 ) cDNA from PHA activated immune cells . ^^^ Using mRNA differential display and 5 ' RACE we isolated human HDAC 3 , a novel gene that is upregulated in PHA activated T cell clones . ^^^ HDAC 3 is homologous to other human HDACs and yeast RPD 3 . ^^^ In contrast , GMCSF downregulated PBMC levels of HDAC 3 mRNA . ^^^ In human myeloid leukemia THP 1 cells , HDAC 3 transfection resulted in increased size , aberrant nuclear morphology and cell cycle G2 / M cell accumulation . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Characterization of a human RPD 3 ortholog , HDAC 3 . ^^^ We have screened the expressed sequence tag database ( National Center for Biotechnology Information ) with the yeast RPD 3 sequence and identified a human ortholog of RPD 3 , HDAC 3 . ^^^ Immunoprecipitation experiments with either an antiserum against HDAC3p or an anti FLAG antiserum and a flagged HDAC 3 cDNA showed that HDAc3p exhibits deacetylase activity both on free histones and on purified nucleosomes . ^^^ Cloning of the cDNA for a human histone deacetylase ( HDAC 1 ) has shown that it represents a human ortholog of the yeast transcriptional regulator RPD 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Herein we report that CBHA and SAHA inhibit histone deacetylase 1 ( HDAC 1 ) and histone deacetylase 3 ( HDAC 3 ) activity in vitro . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Antibodies directed against endogenous HDAC 1 , HDAC 2 , or HDAC 3 immunoprecipitate histone deacetylase activity that is inhibited in vitro by the small molecule trapoxin ( TPX ) , and all three HDACs are retained by a TPX affinity matrix . ^^^ HDAC 1 and HDAC 2 are associated in HeLa cells in a complex that is predominantly separate from an HDAC 3 immune complex . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Here we describe the cloning , sequencing and genetic mapping of two histone deacetylase genes in Drosophila melanogaster : dHDAC 1 is essentially identical to the previously cloned D . melanogaster d Rpd 3 gene and dHDAC 3 , a novel gene , is orthologous to the human and the chicken ( Gallus gallus ) HDAC 3 genes . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
SpHDAC 1 , a cDNA homolog of the yeast Rpd 3 and higher eukaryotic histone deacetylases ( HDAC ) , was cloned from the sea urchin Strongylocentrotus purpuratus . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The histone deacetylase domain of almost all members of higher eukaryotic histone deacetylases already identified ( HDAC family ) is highly homologous to that of yeast RPD 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Human HDAC 1 , HDAC 2 , and HDAC 3 proteins are members of the first class , whereas no class 2 human HDAC proteins had been identified . ^^^ However , HDAC 4 and HDAC 5 associate with HDAC 3 in vivo . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
HDRP does not possess intrinsic HDAC activity but forms complexes with both HDAC 1 and HDAC 3 . ^^^ To date , six HDACs have been identified in mammalian cells : the yeast RPD 3 homologs HDAC 1 , 2 , and 3 and the yeast HDA 1 homologs HDAC 4 , 5 , and 6 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Loss of this interaction allows HDAC 4 and HDAC 5 to translocate to the nucleus , interact with HDAC 3 , and repress gene expression . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
We find that both 5 ErbA and TR can recruit the corepressor N CoR , but , in contrast to existing models , show a concomitant enrichment for HDAC 3 that occurs without an association with Sin 3 , HDAC1 / RPD3 , Mi 2 or HDAC 5 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Here we present the complete nucleotide sequence of a cDNA clone , termed HDAC 8 , that encodes a protein product with similarity to the RPD 3 class ( 1 ) of HDACs . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Direct interaction of RIP 140 with HDAC 1 and HDAC 3 occurs in vitro and in vivo as demonstrated in co immunoprecipitation and glutathione S transferase pull down experiments . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Based on Western blotting using antibodies against known subunits , the only HDAC found in the N CoR 1 complex was HDAC 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Two genes appear to participate in feedback loops that modulate HDAC activity : ZRT 1 encodes a zinc transporter and is repressed by RPD 3 ( Rpd3p is zinc dependent ) ; BNA 1 encodes a nicotinamide adenine dinucleotide ( NAD ) biosynthesis enzyme and is repressed by SIR 2 ( Sir2p is NAD dependent ) . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
In vitro , N CoR can interact with the Sin 3 corepressor , which in turn binds to the histone deacetylase Rpd 3 ( HDAC 1 ) , predicting the existence of a corepressor complex containing N CoR , Sin 3 , and histone deacetylase . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Furthermore , the same HDAC 1 domain is also necessary for in vitro binding of HDAC 2 and HDAC 3 , association with RbAp 48 and for catalytic activity of the enzyme . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The Rpd 3 histone deacetylase ( HDAC ) functions in a large complex containing many proteins including Sin 3 and Sap 30 . ^^^ Based on these observations , we explored whether Pho 23 is a component of the Rpd 3 HDAC complex . ^^^ Furthermore , similar levels of HDAC activity were detected in immunoprecipitates of HA Pho 23 , HA Rpd 3 , or HA Sap 30 . ^^^ In contrast , HDAC activity was not detected in immunoprecipitates of HA Pho 23 or HA Sap 30 from strains lacking Rpd 3 , suggesting that Rpd 3 is the HDAC associated with these proteins . ^^^ However , HDAC activity was detected in immunoprecipitates of HA Sap 30 or HA Rpd 3 from cells lacking Pho 23 , although levels were significantly lower than those detected in wild type cells , indicating that Rpd 3 activity is compromised in the absence of Pho 23 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Human HDAC 7 histone deacetylase activity is associated with HDAC 3 in vivo . ^^^ The HDAC activity of HDAC 7 maps to a carboxyl terminal domain and is dependent on the interaction with the class 1 HDAC , HDAC 3 , in the cell nucleus . ^^^ Cytoplasmic HDAC 7 that is not bound to HDAC 3 is enzymatically inactive . ^^^ We provide evidence that the transcriptional corepressors SMRT and N CoR could serve as critical mediators of HDAC 7 activity by binding class 2 HDACs and HDAC 3 by two distinct repressor domains . ^^^ Different class 2 HDACs reside in the cell nucleus in stable and autonomous complexes with enzymatic activity , but the enzymatic activities associated with HDAC 7 and HDAC 4 rely on shared cofactors , including HDAC 3 and SMRT / N CoR . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Previous studies have established that YY 1 interacts with histone acetyltransferases p 300 and CREB binding protein ( CBP ) and histone deacetylase 1 ( HDAC 1 ) , HDAC 2 , and HDAC 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
In vivo , HDAC 3 forms a stable complex with the SMRT corepressor . ^^^ The SMRT HDAC 3 complex exhibits histone deacetylase activity , whereas recombinant HDAC 3 is an inactive enzyme . ^^^ Here we report that SMRT functions as an activating cofactor of HDAC 3 . ^^^ Activation of HDAC 3 is mediated by a deacetylase activating domain ( DAD ) that includes one of two SANT motifs present in SMRT . ^^^ A cognate DAD is present in the related corepressor N CoR , which can also activate HDAC 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
In addition , of eight histone deacetylases ( HDACs ) tested , only the class 1 HDACs HDAC 1 , HDAC 2 , and HDAC 3 bind ETO . ^^^ Furthermore , ETO 2 binds HDAC 1 , HDAC 2 , and HDAC 3 but also interacts with HDAC 6 and HDAC 8 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
GATA 2 directly associates with HDAC 3 but not with HDAC 1 . ^^^ Consistent with this , HDAC 3 suppressed the transcriptional potential of GATA 2 , whereas HDAC 1 did not affect GATA 2 dependent transcription . ^^^ Results further demonstrated that GATA 2 and HDAC 3 colocalized in the nucleus . ^^^ These results identify GATA 2 as a nuclear target for HDAC 3 mediated repression . ^^^ This is the first demonstration that a tissue specific transcription factor directly and selectively interacts with HDAC 3 and HDAC 5 among HDAC family members . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Complexes shown to contain HDAC 1 , HDAC 3 , HDAC 6 , and HDAC1+2 as their catalytic subunits have been used in an antibody based assay that detects deacetylation of whole histones at defined lysines . ^^^ In comparison to HDAC 1 , HDAC 3 preferentially deacetylated lysines 5 and 12 of H 4 and lysine 5 of H2A . ^^^ H 4 tails in purified mononucleosomes were refractory to deacetylation by both HDAC 1 and HDAC 3 , unless ATP was added to the reaction mix . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
In humans , four highly homologous class 1 HDAC enzymes ( HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 8 ) have been identified to date . ^^^ Although HDAC 3 shares some structural and functional similarities with other class 1 HDACs , it exists in multisubunit complexes separate and different from other known HDAC complexes , implying that individual HDACs might function in a distinct manner . ^^^ In this current study , to understand further the cellular function of HDAC 3 and to uncover possible unique roles this protein may have in gene regulation , we performed a detailed analysis of HDAC 3 using deletion mutations . ^^^ Surprisingly , we found that the non conserved C terminal region of HDAC 3 is required for both deacetylase and transcriptional repression activity . ^^^ In addition , we discovered that the central portion of the HDAC 3 protein possesses a nuclear export signal , whereas the C terminal part of HDAC 3 contributes to the protein ' s localization in the nucleus . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Enzymatic activity associated with class 2 HDACs is dependent on a multiprotein complex containing HDAC 3 and SMRT / N CoR . ^^^ Here , we show that the catalytic domain of HDAC 4 interacts with HDAC 3 via the transcriptional corepressor N CoR / SMRT . ^^^ All experimental conditions leading to the suppression of HDAC 4 binding to SMRT / N CoR and to HDAC 3 result in the loss of enzymatic activity associated with HDAC 4 . ^^^ In vitro reconstitution experiments indicate that HDAC 4 and other class 2 HDACs are inactive in the context of the SMRT / N CoR HDAC 3 complex and do not contribute to its enzymatic activity . ^^^ These observations indicate that class 2 HDACs regulate transcription by bridging the enzymatically active SMRT / N CoR HDAC 3 complex and select transcription factors independently of any intrinsic HDAC activity . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Non permissive cells contain the class 1 HDAC , HDAC 3 ; super expression of HDAC 3 in normally permissive cells reduces infection and MIEP activity . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Similar mechanisms appear to be operative in yeast , in which epistasis experiments have established that the mSin 3 and HDAC orthologs ( SIN 3 and RPD 3 ) , along with a novel protein , SDS 3 , function in the same repressor pathway . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
A particular pair of HAT ( Esa 1 ) and HDAC ( Rpd 3 ) is proposed to modify the same lysine residue in vitro and in vivo . ^^^ Here we show that HAT ( Esa 1 family ) and HDAC ( Rpd 3 family ) have similar amino acid stretches in the primary structures through evolution . ^^^ We did alanine scanning mutagenesis and found that the ER motif regions of Esa 1 or Rpd 3 are required for HAT activity of Esa 1 or HDAC activity of Rpd 3 , respectively . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Here we report on the cloning of the first P . polycephalum HDAC ( PpHDAC 1 ) related to the S . cerevisiae Rpd 3 protein . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
PPARgamma and RB were shown to coimmunoprecipitate , and this PPARgamma RB complex also contains the histone deacetylase HDAC 3 , thereby attenuating PPARgamma ' s capacity to drive gene expression and adipocyte differentiation . ^^^ Dissociation of the PPARgamma RB HDAC 3 complex by RB phosphorylation or by inhibition of HDAC activity stimulates adipocyte differentiation . ^^^ These observations underscore an important function of both RB and HDAC 3 in fine tuning PPARgamma activity and adipocyte differentiation . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Histone deacetylase 3 ( HDAC 3 ) requires the nuclear receptor corepressor SMRT for HDAC enzyme activity . ^^^ Histone deacetylase 3 ( HDAC 3 ) requires the nuclear receptor corepressor SMRT for HDAC enzyme activity . ^^^ Here we report that HDAC 3 interacts with SMRT only after priming by cellular chaperones including the TCP 1 ring complex ( TRiC ) , which is required for proper folding of HDAC 3 in an ATP dependent process . ^^^ SMRT displaces TRiC from HDAC 3 , yielding an active HDAC enzyme . ^^^ The SMRT HDAC 3 repression complex thus joins the VHL elongin BC tumor suppression complex and the cyclin E Cdk 2 cell cycle regulation complex as critical cellular machines requiring TRiC for proper assembly and function . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
By means of quantitative reverse transcription polymerase chain reaction using SYBR Green to detect double stranded DNA , mRNA expression profiles for histone deacetylases ( HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 7 ) , histone acetyltransferases ( GCN 5 and HAT 1 ) , and histone H2A were established . ^^^ The HDAC 3 ( class 1 HDAC ) tends to have an expression profile similar to those of HDAC 1 , HDAC 2 , and HAT 1 , whereas the HDAC 7 ( class 2 HDAC ) and GCN 5 ( type A HAT ) profiles were different from those three . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The OsHDAC 1 gene encoded a protein of approximately 57 kDa that shared 73 . 5 , 72 . 7 , 79 . 9 , and 57 . 1 % amino acid sequence identity with the OsHDAC 2 , OsHDAC 3 , maize RPD 3 , and human HDAC 1 proteins , respectively . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Western blot analysis of these complexes suggests that hCtBP 1 associates with class 1 HDACs , HDAC 1 , HDAC 2 and HDAC 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The results show that an inhibitor of HDACs , trichostatin A , enhances IFN gamma induced MHC 2 expression , while HDAC1 / HDAC2 inhibits IFN gamma and CIITA induced MHC 2 gene expression . mSin3A , a corepressor of HDAC1 / HDAC2 , is important for this inhibition , while NcoR , a corepressor of HDAC 3 , is not . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Through the use of HDAC inhibitors and coimmunoprecipitations , we furthermore demonstrate that the effects of RB on PPARgamma mediated control of the cell cycle and apoptosis depend on the recruitment of histone deacetylase 3 ( HDAC 3 ) to PPARgamma . ^^^ In combination , these data hence demonstrate that the effects of PPARgamma on cell proliferation and apoptosis are dependent on the presence of an RB HDAC 3 complex . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The N terminal SANT is a critical component of a deacetylase activation domain ( DAD ) that binds and activates HDAC 3 . ^^^ The HID enhances repression by increasing the affinity of the DAD HDAC 3 enzyme for histone substrate . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Cytoplasmic IkappaBalpha increases NF kappaB independent transcription through binding to histone deacetylase ( HDAC ) 1 and HDAC 3 . ^^^ We show here that IkappaBalpha enhances the transactivation potential of several homeodomain containing proteins such as HOXB 7 and Pit 1 through a NF kappaB independent association with histone deacetylase ( HDAC ) 1 and HDAC 3 but not with HDAC 2 , 4 , 5 , and 6 . ^^^ Immunofluorescence experiments demonstrated further that IkappaBalpha acts by partially redirecting HDAC 3 to the cytoplasm . ^^^ Our results support the hypothesis that the NF kappaB inhibitor IkappaBalpha regulates the transcriptional activity of homeodomain containing proteins positively through cytoplasmic sequestration of HDAC 1 and HDAC 3 , a mechanism that would assign a new and unexpected role to IkappaBalpha . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
To further explore the catalytic activity of HDACs , we expressed two additional human class 1 HDACs , HDAC 1 and HDAC 3 , in baculovirus . ^^^ Recombinant HDAC 1 and HDAC 3 fusion proteins remained soluble and catalytically active and were purified to near homogeneity . ^^^ Interestingly , trichostatin ( TSA ) was found to be a potent inhibitor for all three HDACs ( IC 50 value of approximately 0 . 1 0 . 3 microM ) , whereas another HDAC inhibitor MS 27 275 ( N ( 2 aminophenyl ) 4 [ N ( pyridin 3 methyloxycarbonyl ) aminomethyl ] benzamide ) preferentially inhibited HDAC 1 ( IC 50 value of approximately 0 . 3 microM ) versus HDAC 3 ( IC 50 value of approximately 8 microM ) and had no inhibitory activity toward HDAC 8 ( IC 50 value > 100 microM ) . ^^^ HDAC 1 protein was more abundantly expressed in SW 620 cells compared with that of HDAC 3 and HDAC 8 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The siRNA for HDAC 3 and HDAC 1 demonstrated significant morphological changes in HeLa S 3 consistent with those observed with HDAC inhibitors . ^^^ HDAC 3 siRNA caused histone hyperacetylation and increased the percent of apoptotic cells . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
To test the hypothesis that the delay reflected a dysfunction of ICP 0 in altering the structure of host protein viral DNA complexes , we examined the state of histone deacetylases ( HDACs ) ( HDAC 1 , HDAC 2 , and HDAC 3 ) . ^^^ We report the following . ( 1 ) HDAC 1 and HDAC 2 , but not HDAC 3 , were modified in infected cells . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
In the present study , we investigated whether class 1 and class 2 HDACs interact with GATA 1 to regulate its function and indeed , GATA 1 is directly associated with HDAC 3 , HDAC 4 and HDAC 5 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Promoter bound OHT complexed ERa coordinately recruited the components of a multiprotein complex containing the corepressor NCoR , histone deacetylase 3 ( HDAC 3 ) , and a WD 40 repeat protein TBL 1 . ^^^ Kinetic studies revealed that following OHT addition the recruitment of these HDAC complexes to pS 2 or the c myc promoter occurs in a sequential manner ; the NCoR HDAC 3 complex is recruited earlier than the NuRD complex . ^^^ Serial ChIP experiments indicated that the ER NCoR HDAC 3 and ER NuRD complexes are distinct , and they do not occupy the target gene promoter simultaneously . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Expression and functional characterization of recombinant human HDAC 1 and HDAC 3 . ^^^ Here we present the stable expression of human recombinant His tagged HDAC 1 and HDAC 3 in mammalian cells . ^^^ Full length human genes for HDAC 1 and HDAC 3 were cloned into the pcDNA 3 . 1 vector containing a N terminal His tag with an enterokinase cleavage site . ^^^ Western blots demonstrated the nickel affinity purified rhHDAC 1 preparation also contained endogenous HDAC 2 and HDAC 3 ; likewise , rhHDAC 3 preparation contained endogenous HDAC 1 and HDAC 2 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Consistent with prior studies , we find that RPD 3 , which encodes a histone deacetylase ( HDAC ) , is required for repression upon rapamycin treatment . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Activation of the growth differentiation factor 11 gene by the histone deacetylase ( HDAC ) inhibitor trichostatin A and repression by HDAC 3 . ^^^ A comprehensive survey of human HDACs revealed that HDAC 3 is necessary and sufficient for the repression of gdf 11 promoter activity . ^^^ Chromatin immunoprecipitation assays showed that treatment of cells with TSA or silencing of HDAC 3 expression by small interfering RNA causes the hyperacetylation of Lys 9 in histone H 3 on the gdf 11 promoter . ^^^ Together , our results provide a new model in which HDAC inhibitors reverse abnormal cell growth by inactivation of HDAC 3 , which in turn leads to the derepression of gdf 11 expression . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
In transient transfection assays , HDAC 3 repressed Runx 2 mediated activation of the osteocalcin promoter . ^^^ HDAC inhibitors and HDAC 3 specific short hairpin RNAs reversed this repression . ^^^ In vivo , Runx 2 and HDAC 3 associated with the osteocalcin promoter . ^^^ These data indicate that HDAC 3 regulates Runx 2 mediated transcription of osteoblast genes . ^^^ Suppression of HDAC 3 in MC3T3 preosteoblasts by RNA interference accelerated the expression of Runx 2 target genes , osteocalcin , osteopontin , and bone sialoprotein but did not significantly alter Runx 2 levels . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Results further indicated that HDAC 3 decreases the MAPK 11 phosphorylation state and inhibits the activity of the MAPK 11 dependent transcription factor , activating transcription factor 2 ( ATF 2 ) . ^^^ LPS mediated activation of ATF 2 was inhibited by HDAC 3 in a time and dose dependent manner . ^^^ Inhibition of HDAC 3 expression by RNA interference resulted in increased ATF 2 activation in response to LPS stimulation . ^^^ In agreement with decreased ATF 2 transcriptional activity by HDAC 3 , HDAC 3 repressed TNF gene expression , and TNF protein production observed in response to LPS stimulation . ^^^ Therefore , our results indicate that HDAC 3 interacts directly and selectively with MAPK 11 , represses ATF 2 transcriptional activity , and acts as a regulator of TNF gene expression in LPS stimulated cells , especially in mononuclear phagocytes . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Consistent with these findings , IE 1 interacts specifically with HDAC 3 within infected cells . ^^^ We also demonstrate an interaction between IE 2 and HDAC 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The histone deacetylase inhibitor suberoylanilide hydroxamic acid down regulates expression levels of Bcr abl , c Myc and HDAC 3 in chronic myeloid leukemia cell lines . ^^^ Even though earlier reports on SAHA considerably focused on its inhibitory effect on HDAC enzymatic activity , we report herein a significant down regulation of HDAC 3 protein expression levels following treatment with SAHA in BV 173 cells , but not in K 562 cells . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The N terminal region directs hr to a speckled nuclear pattern that co localizes with the histone deacetylase 3 ( HDAC ) , but not with HDAC 1 or HDAC 7 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The yeast histone acetyltransferase ( HAT ) gene gcn 5 and histone deacetylase ( HDAC ) gene rpd 3 were cloned from yeast genomic DNA by PCR amplification . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The interaction between PPARgamma and RB decreases the transcriptional activity of PPARgamma through recruitment of the histone deacetylase HDAC 3 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Cyclin D 1 bound HDAC in vivo and preferentially physically associated with HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 5 . ^^^ Chromatin immunoprecipitation assay demonstrated that cyclin D 1 enhanced recruitment of HDAC 1 and HDAC 3 and histone methyltransferase SUV39H1 to the PPAR response element of the lipoprotein lipase promoter and decreased acetylation of total histone H 3 and histone H 3 lysine 9 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
HDAC 1 und HDAC 3 protein expression was analyzed immunohistochemically on a tissue microarray ( TMA ) containing 600 core biopsies from 200 patients . ^^^ Moderate or strong nuclear immunoreactivity for HDAC 1 was observed in 39 . 8 % and for HDAC 3 in 43 . 9 % of breast carcinomas . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Cell fractionation assays performed with primary human smooth muscle cells ( HSMCs ) showed that HDAC 8 , in contrast to HDAC 1 and HDAC 3 , was enriched in cytoskeleton bound protein fractions and insoluble cell pellets , suggesting an association of HDAC 8 with the cystoskeleton . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Histone deacetylase 3 ( HDAC 3 ) activity is regulated by interaction with protein serine / threonine phosphatase 4 . ^^^ Histone deacetylase 3 ( HDAC 3 ) is one of four members of the human class 1 HDACs that regulates gene expression by deacetylation of histones and nonhistone proteins . ^^^ Histone deacetylase 3 ( HDAC 3 ) activity is regulated by interaction with protein serine / threonine phosphatase 4 . ^^^ Histone deacetylase 3 ( HDAC 3 ) is one of four members of the human class 1 HDACs that regulates gene expression by deacetylation of histones and nonhistone proteins . ^^^ Early studies have suggested that HDAC 3 activity is regulated by association with the corepressors N CoR and SMRT . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
CBZ inhibited HDAC 3 and HDAC 7 , which are representatives of HDAC class 1 and 2 respectively . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Using immunoscreening of tumor derived cDNA expression libraries ( SEREX ) , we identified human histone deacetylase 3 ( HDAC 3 ) as serologically defined antigen in colon cancer . ^^^ We show that the C terminal region of HDAC 3 protein lacking the homology to other Class 1 HDAC contains at least 3 distinct B cell epitopes that are recognized by the serum antibodies . ^^^ HDAC 3 in combination with other SEREX antigens may become a useful molecular biomarker with diagnostic or prognostic value for a subset of colon cancer patients . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
HDAC 3 but not HDAC 1 or HDAC 2 was required for AP 1 mediated stimulation of c jun expression . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Comparison with gene microarray data revealed that 31 % of the differentially regulated proteins correlate with altered mRNA expression in squamous cell lung carcinomas , including PEX 1 , MKK 7 and HDAC 3 for up regulated proteins . ^^^ The histone deacetylase ( HDAC ) 3 was investigated in detail by immunoblot analysis showing that HDAC 3 is indeed elevated in 92 % of tumours ( n=22 / 24 ; P < 0 . 001 ) . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
We found that histone deacetylase 3 ( HDAC 3 ) was specifically induced after hyperthermia at 44 degrees C for 30 min at pH 6 . 7 . ^^^ Although the cytotoxicity of heating at 44 degrees C for 30 min was enhanced by decreasing the pH from 7 . 4 to 6 . 7 , it was enhanced even more by antisense RNA oligonucleotides for HDAC 3 . ^^^ The inhibition of HDAC 3 by the antisense RNA oligonucleotides suppressed partially the induction of G2 / M arrest , resulting in an enhancement of the apoptosis caused by the heating under acidic conditions . ^^^ Antisense RNA oligonucleotides for HDAC 3 enhanced apoptosis 48 h after hyperthermia at 43 degrees C for 30 min in vivo . ^^^ HDAC 3 may be a novel target enhancing hyperthermia and combined treatment with hyperthermia and HDAC inhibitors is a possible modality for cancer therapy . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
The nuclear envelope protein and transcriptional repressor LAP2beta interacts with HDAC 3 at the nuclear periphery , and induces histone H 4 deacetylation . ^^^ We further show that LAP2beta interacts at the nuclear envelope with HDAC 3 , a class 1 histone deacetylase , and that TSA ( an HDAC inhibitor ) abrogates LAP2beta ' s repressive activity . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
To understand the physiological functions of HDACs , we characterized six different Drosophila HDACs , including Rpd 3 , HDAC 3 , HDAC 4 , HDAC 6 S , HDAC 6 L , and Sir 2 , by developmental expression pattern , transcriptional profiles of target genes , and sensitivity to HDAC inhibitors . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Association of HDAC 3 and HDAC 1 with the relB VDR binding site was observed , but only HDAC 3 was reciprocally modulated by D ( 3 ) analog and LPS . ^^^ Overexpression of HDAC 3 caused relB promoter suppression , increased sensitivity to D ( 3 ) analog , and resistance to LPS . ^^^ Depletion of HDAC 3 attenuated relB suppression by D ( 3 ) analog . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
To understand the difference between class 1 HDAC isoforms that could be exploited for the design of isoform specific HDAC inhibitors , we have built three dimensional models of four class 1 histone deacetylases , HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 8 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
PML RARalpha recruits MBD 1 to its target promoter through an HDAC 3 mediated mechanism . ^^^ Binding of HDAC 3 and MBD 1 is not confined to the promoter region but instead is spread over the locus . ^^^ Knock down of HDAC 3 expression by RNA interference in acute promyelocytic leukemia cells alleviates PML RAR induced promoter silencing . ^^^ Our results demonstrate that PML RARalpha functions by recruiting an HDAC 3 MBD1 complex that contributes to the establishment and maintenance of the silenced chromatin state . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
CSE also reduced histone deacetylase ( HDAC ) activity and HDAC 1 , HDAC 2 , and HDAC 3 protein levels . ^^^ This was associated with posttranslational modification of HDAC 1 , HDAC 2 , and HDAC 3 protein by nitrotyrosine and aldehyde adduct formation . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
We also demonstrate that this marker of functional HDAC inhibition occurs almost immediately ( 15 min ) after exposure of F 9 cells to VPA , whereas no influence on the HDAC protein levels ( HDAC 2 and HDAC 3 ) could be detected even after 24 h of treatment . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Neuronal expression of hda 3 restored Htn Q 150 toxicity and suggested that C . elegans HDAC 3 ( HDA 3 ) acts within neurons to promote degeneration in response to Htn Q 150 . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Glutathione S transferase ( GST ) pull down assays further verified direct interaction between GCMa and HDAC 3 or CBP and HDAC 3 . ^^^ HDAC 3 counteracts the transcriptional coactivator activity of CBP and the enhancement effect of CBP on GCMa mediated transcriptional activation . ^^^ Correlatively , we found in placental cells that HDAC 3 associates with the proximal GCMa binding site ( pGBS ) in the syncytin promoter and dissociates from pGBS in the presence of forskolin , which stimulates the association of CBP and GCMa with pGBS . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Histone deacetylase 3 ( HDAC 3 ) and other class 1 HDACs regulate colon cell maturation and p 21 expression and are deregulated in human colon cancer . ^^^ Histone deacetylase 3 ( HDAC 3 ) and other class 1 HDACs regulate colon cell maturation and p 21 expression and are deregulated in human colon cancer . ^^^ Whereas a role for HDAC 1 and 2 in mediating components of the HDAC inhibitor response has been reported , the role of HDAC 3 is unknown . ^^^ Here we demonstrate increased protein expression of HDAC 3 in human colon tumors and in duodenal adenomas from Apc 1638 ( N / + ) mice . ^^^ HDAC 3 was also maximally expressed in proliferating crypt cells in normal intestine . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Involvement of the SMRT / NCoR HDAC 3 complex in transcriptional repression by the CNOT 2 subunit of the human Ccr 4 Not complex . ^^^ We found that coexpression of SMRT ( silencing mediator for retinoic acid receptor and thyroid hormone receptor ) or NCoR ( nuclear hormone receptor co repressor ) in combination with HDAC 3 ( or HDAC 5 and HDAC 6 ) augmented the repression by CNOT 2 . ^^^ We observed physical interactions of CNOT 2 with several subunits of the SMRT / NCoR HDAC 3 complex . ^^^ Our results show that the SMRT / NCoR HDAC 3 complex is a cofactor of CNOT 2 mediated repression and suggest that transcriptional regulation by the Ccr 4 Not complex involves regulation of chromatin modification . . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Significantly , E1A antisense was shown to co eliminate E1A 12 as well as HDAC 1 and HDAC 8 , but not HDAC 3 , from the enhancer repression complex . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Using different HDACi together with small interfering RNA for HDAC 1 , HDAC 2 , HDAC 3 , and HDAC 6 , we report that inhibition of HDAC 1 and HDAC 2 but not HDAC 3 , HDAC 6 , and HDAC 8 are primarily responsible for sensitization to TRAIL induced apoptosis . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
In agreement , HDAC 3 transfection led to reductions in both Tax expression and histone acetylation . ^^^ HDAC 3 mutations and deletions spanning the catalytic site had variable ability to repress Tax , but HDAC activity was not essential for repression . ^^^ Immunoprecipitation studies revealed that Tax co exists in a complex containing both histone deacetylase 1 ( HDAC 1 ) and 3 ( HDAC 3 ) . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Unliganded thyroid hormone receptor ( TR ) actively represses transcription via the nuclear receptor corepressor ( N CoR ) / histone deacetylase 3 ( HDAC 3 ) complex . ^^^ N CoR and HDAC 3 are both required for recruitment of SNF2H to the repressed gene . ^^^ Indeed , HDAC 3 as well as SNF2H are required for nucleosomal organization on the TR target gene . ^^^
Interacting proteins: Q9UKV0 and O15379	Pubmed	SVM Score :0.0
Here we report that HDAC 3 is a critical , transcription independent regulator of mitosis . ^^^ HDAC 3 forms a complex with A Kinase Anchoring Proteins AKAP 95 and HA 95 , which are targeted to mitotic chromosomes . ^^^ Deacetylation of H 3 in mitosis requires AKAP95 / HA95 and HDAC 3 and provides a hypoacetylated H 3 tail that is the preferred substrate for Aurora B kinase . ^^^